Standard name
Description Lysine-specific metalloprotease of the pitrilysin family; metalloprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0.05 0.06 0 0 0 0 0 0 0.05 0.05 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.11 0.06 0.08 0.05 0.05 0.05 0.05 0 0
Golgi 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.13 0.27 0.24 0.05 0.05 0 0.09 0.15 0.1
Mitochondria 0.74 0.88 0.95 0.93 0.9 0.94 0.9 0.94 0.92 0.93 0.95 0.96 0.85 0.87 0.91 0.05 0.12 0.09 0.82 0.74 0.83 0.7 0.6 0.63
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0.05 0 0 0.06 0.1 0.12 0.1 0.11 0.09 0.12 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.05 0.07 0.09 0.07 0.07 0.07 0.07 0.07 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Peroxisomes 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0.28 0.25 0.22 0.05 0 0.06 0.06 0.09 0.12
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.14 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.15 0.07 0 0 0 0 0 0.05 0 0.05 0 0.11 0.08 0.1 0.23 0.18 0.13 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 2 3 4 0 1 1 0 0 0 0 0 3 2 0 2 0 3 4 1 5 10 13
Bud 0 4 17 18 15 14 15 14 9 13 16 27 3 4 8 10 0 5 0 1 0 1 2 1
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0
Bud Site 0 1 4 1 1 3 4 2 5 5 5 4 0 0 1 3 1 0
Cell Periphery 5 10 3 4 5 1 1 9 5 7 3 10 7 13 7 10 8 8 0 0 0 0 0 0
Cytoplasm 1 8 2 17 7 9 15 10 12 7 6 4 7 9 9 6 5 16 0 2 0 0 0 0
Endoplasmic Reticulum 0 0 1 2 2 2 4 5 4 0 7 10 1 1 0 4 5 4 0 0 0 0 1 2
Endosome 21 7 3 1 9 3 6 3 0 0 0 2 14 10 7 19 9 9 13 17 17 11 8 5
Golgi 30 1 0 0 2 1 1 2 0 0 0 0 4 23 4 22 42 28 14 17 7 19 36 25
Mitochondria 192 269 325 297 334 385 474 527 384 383 320 499 202 264 292 8 19 11 242 233 292 152 143 164
Nucleus 1 1 1 0 2 2 5 3 5 4 1 2 1 1 0 3 3 0 0 0 0 1 0 0
Nuclear Periphery 16 15 14 11 21 39 61 59 47 39 40 63 4 1 7 7 2 0 2 2 2 1 2 1
Nucleolus 19 7 8 6 19 28 49 42 29 28 25 34 3 5 3 9 7 4 1 0 0 1 1 4
Peroxisomes 19 11 19 6 15 6 5 6 0 6 5 2 13 24 8 48 38 26 14 11 19 12 20 31
SpindlePole 5 1 5 1 1 6 6 2 1 2 3 6 5 2 2 28 22 12 1 1 2 0 0 0
Vac/Vac Membrane 42 46 25 9 13 10 19 24 20 13 18 15 26 25 31 39 28 15 1 15 2 6 5 3
Unique Cell Count 260 307 342 320 370 410 525 563 416 412 337 519 238 304 322 173 155 117 297 315 350 217 238 261
Labelled Cell Count 354 381 430 376 450 509 666 709 521 507 449 678 290 385 381 216 192 138 297 315 350 217 238 261


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 7.7 5.7 6.1 7.2 7.2 7.0 7.0 6.5 6.3 6.1 5.9 8.6 7.9 6.8 9.2 10.5 10.5 9.1 9.5 10.1
Std Deviation (1e-4) 1.9 1.7 1.0 1.4 1.4 1.7 1.8 1.6 1.9 1.9 1.6 1.6 6.5 1.4 1.4 1.9 2.6 2.7 2.0 2.3 2.2
Intensity Change (Log2) 0.11 0.35 0.35 0.3 0.29 0.2 0.16 0.1 0.05 0.6 0.47 0.27 0.69 0.88 0.89 0.67 0.75 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.4 -0.6 -1.1 -1.6 -2.0 -2.1 -1.3 -0.1 0.2 -2.4 -2.6 -1.7 0.4 -2.8 -0.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.2 0 2.8 0 0 0 0
Cytoplasm 3.6 0 1.8 2.4 0 2.3 0 0 0 0 2.3 2.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.6 0 0 0 0 0 0 0 3.5 2.2 0 5.4 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 5.2 0 6.7 10.1 9.3
Mitochondria -1.2 -2.4 -0.7 -2.5 -0.9 -1.5 -1.2 0 0.8 -4.2 -3.7 -2.2 -20.3 -18.5 -18.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.4 1.0 2.9 3.9 3.4 3.6 2.9 3.7 4.0 -1.6 -3.2 -1.4 0 0 0
Nucleolus -0.4 2.0 2.9 4.1 3.3 2.9 2.9 3.1 2.8 0 -0.6 -1.4 1.7 0 0
Peroxisomes -2.5 -0.9 -3.1 -4.0 -4.0 -4.9 -3.1 -2.9 -4.8 0 1.2 -2.0 7.1 6.1 5.2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 6.4 5.8 0
Vacuole -2.6 -2.3 -3.2 -2.4 -2.0 -1.5 -2.6 -1.1 -3.0 1.5 0.4 1.1 4.9 3.6 1.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cym1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available