Standard name | |
---|---|
Human Ortholog | |
Description | Dual endo- and exopolyphosphatase with a role in phosphate metabolism; acts as both an endopolyphosphatase cleaving long chains of polyphosphate distributively to generate shorter polymer chains and as an exopolyphosphatase catalyzing the hydrolysis of terminal phosphate from polyphosphate; localizes to the vacuole, nucleus and cytosol; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 33 | 94 | 82 | 108 | 98 | 111 | 167 | – | 152 | 196 | 208 | 185 | 117 | 202 | 248 | 49 | 59 | 59 | 2 | 7 | 10 | 4 | 25 | 26 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 0 | 9 | 2 | 1 | 1 | 0 | 3 | 10 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 6 | 1 | 3 | 9 | 1 | 3 | – | 2 | 1 | 0 | 0 | 0 | 2 | 2 | 12 | 3 | 11 | 3 | 10 | 3 | 0 | 4 | 12 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 32 | 5 | 1 | 1 | 3 | 14 | 12 | – | 11 | 16 | 31 | 14 | 0 | 0 | 2 | 7 | 4 | 0 | 1 | 2 | 3 | 2 | 7 | 7 |
Nucleus | 20 | 32 | 18 | 5 | 40 | 64 | 103 | – | 49 | 44 | 20 | 20 | 8 | 1 | 1 | 51 | 46 | 49 | 1 | 3 | 2 | 1 | 3 | 4 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 3 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 68 | 164 | 115 | 92 | 199 | 205 | 242 | – | 189 | 196 | 129 | 134 | 24 | 32 | 13 | 131 | 143 | 148 | 134 | 228 | 177 | 69 | 181 | 182 |
Unique Cell Count | 123 | 235 | 183 | 167 | 280 | 291 | 404 | 310 | 334 | 322 | 279 | 135 | 222 | 261 | 199 | 218 | 218 | 149 | 257 | 207 | 84 | 231 | 255 | |
Labelled Cell Count | 153 | 302 | 218 | 210 | 351 | 402 | 530 | 407 | 456 | 401 | 358 | 150 | 239 | 266 | 262 | 267 | 276 | 149 | 257 | 207 | 84 | 231 | 255 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.2 | 6.1 | 6.3 | 7.6 | 6.5 | 6.6 | – | 6.0 | 6.1 | 5.2 | 5.3 | 5.5 | 5.5 | 5.2 | 8.0 | 8.3 | 7.6 | 5.4 | 5.4 | 6.0 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.4 | 1.7 | 2.1 | 1.6 | 1.7 | – | 1.5 | 2.2 | 1.2 | 1.0 | 1.0 | 1.5 | 1.5 | 1.7 | 1.9 | 1.5 | 0.8 | 1.2 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.32 | 0.1 | 0.12 | – | -0.03 | 0.01 | -0.24 | -0.2 | -0.15 | -0.14 | -0.24 | 0.39 | 0.45 | 0.33 | -0.16 | -0.17 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.7 | -2.1 | -1.4 | -0.8 | – | 0.9 | 3.0 | 4.3 | 4.6 | 7.6 | 10.1 | 11.9 | -4.2 | -3.7 | -3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 2.6 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.6 | 0 | – | 0 | 2.6 | 4.0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -2.6 | 1.4 | 3.4 | 4.3 | – | 1.9 | 1.1 | -1.5 | -1.0 | -1.3 | -4.4 | -4.8 | 4.0 | 3.1 | 3.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.5 | 1.9 | 1.7 | -0.7 | – | -0.4 | -0.9 | -4.9 | -3.1 | -8.0 | -10.1 | -13.2 | 0.6 | 0.6 | 1.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3645 | 0.8425 | 0.684 | 0.4677 | 0.0502 | 0.7717 | -0.3332 | 1.2714 | 0.7674 | 0.5094 | 0.6093 | 0.8907 | -0.2521 | 1.0852 | 0.7957 | 0.8719 | 0.3929 | 0.5861 |
Actin | 0.0117 | 0.0001 | 0.004 | 0.0044 | 0.0011 | 0 | 0.0004 | 0.0013 | 0.0015 | 0.0002 | 0.0046 | 0.0015 | 0.0037 | 0 | 0 | 0.0098 | 0 | 0.0009 |
Bud | 0.0006 | 0.0004 | 0.0007 | 0.0007 | 0.0028 | 0.0004 | 0.0012 | 0.0203 | 0.0012 | 0.0114 | 0.0171 | 0.0054 | 0.0007 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0003 |
Bud Neck | 0.0046 | 0.001 | 0.0022 | 0.0006 | 0.0079 | 0.0018 | 0.0014 | 0.0011 | 0.0023 | 0.0031 | 0.0026 | 0.0031 | 0.0017 | 0.0011 | 0.0016 | 0.0032 | 0.0011 | 0.0019 |
Bud Periphery | 0.001 | 0.0007 | 0.0013 | 0.0022 | 0.003 | 0.0012 | 0.0021 | 0.0085 | 0.0017 | 0.0095 | 0.0025 | 0.0067 | 0.0014 | 0.0008 | 0.0008 | 0.0006 | 0.0007 | 0.0011 |
Bud Site | 0.0094 | 0.0014 | 0.0089 | 0.001 | 0.0827 | 0.0008 | 0.0019 | 0.0131 | 0.0016 | 0.02 | 0.0071 | 0.0023 | 0.0017 | 0.0035 | 0.0018 | 0.0027 | 0.0005 | 0.0008 |
Cell Periphery | 0.0018 | 0.0018 | 0.0029 | 0.001 | 0.0025 | 0.0025 | 0.0032 | 0.0021 | 0.0028 | 0.0031 | 0.0016 | 0.0038 | 0.001 | 0.0015 | 0.0018 | 0.0009 | 0.0027 | 0.0018 |
Cytoplasm | 0.0132 | 0.0273 | 0.0142 | 0.0019 | 0.0034 | 0.0174 | 0.0273 | 0.0358 | 0.0213 | 0.0052 | 0.0169 | 0.0278 | 0.021 | 0.0325 | 0.0244 | 0.0077 | 0.0026 | 0.0134 |
Cytoplasmic Foci | 0.0312 | 0.016 | 0.0219 | 0.0229 | 0.0225 | 0.0106 | 0.0432 | 0.0341 | 0.034 | 0.0501 | 0.0481 | 0.0318 | 0.0207 | 0.0119 | 0.0092 | 0.0101 | 0.0021 | 0.0088 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0045 | 0.0032 | 0.0048 | 0.0011 | 0.0008 | 0.0041 | 0.0081 | 0.0098 | 0.0032 | 0.001 | 0.0044 | 0.0028 | 0.0056 | 0.0092 | 0.0046 | 0.0032 | 0.0012 | 0.0027 |
Endosome | 0.2735 | 0.1732 | 0.2555 | 0.1884 | 0.1251 | 0.2094 | 0.2698 | 0.1698 | 0.213 | 0.2857 | 0.1822 | 0.1552 | 0.2896 | 0.1434 | 0.2284 | 0.2004 | 0.1777 | 0.1847 |
Golgi | 0.018 | 0.0033 | 0.011 | 0.0273 | 0.0336 | 0.0052 | 0.0105 | 0.0076 | 0.0067 | 0.0055 | 0.0137 | 0.0052 | 0.0066 | 0.0019 | 0.005 | 0.0077 | 0.0015 | 0.0028 |
Lipid Particles | 0.0385 | 0.0151 | 0.0228 | 0.0498 | 0.0619 | 0.031 | 0.0624 | 0.0142 | 0.0174 | 0.0445 | 0.0525 | 0.0251 | 0.0152 | 0.0112 | 0.0113 | 0.0164 | 0.0029 | 0.0061 |
Mitochondria | 0.0302 | 0.0174 | 0.0243 | 0.0759 | 0.0183 | 0.0036 | 0.0108 | 0.0255 | 0.0147 | 0.0092 | 0.0063 | 0.0288 | 0.0114 | 0.0027 | 0.0138 | 0.0073 | 0.0006 | 0.003 |
None | 0.0158 | 0.0217 | 0.0369 | 0.0003 | 0.0003 | 0.0075 | 0.0921 | 0.041 | 0.0623 | 0.0435 | 0.0082 | 0.0195 | 0.0173 | 0.0275 | 0.0111 | 0.0006 | 0.0002 | 0.0016 |
Nuclear Periphery | 0.0064 | 0.0043 | 0.0069 | 0.0017 | 0.0021 | 0.003 | 0.0056 | 0.0129 | 0.0056 | 0.0013 | 0.0056 | 0.0097 | 0.0121 | 0.0123 | 0.0121 | 0.0063 | 0.0036 | 0.0126 |
Nucleolus | 0.0112 | 0.0021 | 0.006 | 0.0012 | 0.0059 | 0.0046 | 0.0044 | 0.0061 | 0.0066 | 0.0097 | 0.0039 | 0.0098 | 0.0025 | 0.0042 | 0.0061 | 0.0026 | 0.0033 | 0.0033 |
Nucleus | 0.0096 | 0.0039 | 0.0058 | 0.0013 | 0.0037 | 0.011 | 0.0069 | 0.0208 | 0.0253 | 0.0036 | 0.0057 | 0.0221 | 0.0083 | 0.0141 | 0.0113 | 0.006 | 0.0256 | 0.0117 |
Peroxisomes | 0.0131 | 0.0022 | 0.0026 | 0.0227 | 0.0099 | 0.0009 | 0.0147 | 0.0032 | 0.0048 | 0.0051 | 0.0422 | 0.0094 | 0.004 | 0.0007 | 0.0015 | 0.0029 | 0.0003 | 0.0022 |
Punctate Nuclear | 0.0009 | 0.0004 | 0.0028 | 0.0005 | 0.0007 | 0.0004 | 0.0047 | 0.0056 | 0.0007 | 0.0013 | 0.0017 | 0.0026 | 0.0082 | 0.001 | 0.0007 | 0.0048 | 0.0001 | 0.0023 |
Vacuole | 0.404 | 0.5919 | 0.4673 | 0.4883 | 0.5529 | 0.5664 | 0.3712 | 0.4893 | 0.4809 | 0.4282 | 0.5154 | 0.5317 | 0.4528 | 0.6303 | 0.5412 | 0.5855 | 0.6697 | 0.6286 |
Vacuole Periphery | 0.1002 | 0.1126 | 0.0971 | 0.1069 | 0.059 | 0.1181 | 0.0579 | 0.0778 | 0.0924 | 0.0588 | 0.0575 | 0.0959 | 0.1143 | 0.0897 | 0.113 | 0.1206 | 0.1033 | 0.1093 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 59.3931 | 56.0164 | 49.157 | 58.4262 | 50.8662 | 58.2711 | 73.8758 | 82.7696 | 78.3417 | 67.4112 |
Translational Efficiency | 0.677 | 0.6243 | 0.7572 | 0.63 | 0.6131 | 0.6942 | 0.6118 | 0.4709 | 0.5146 | 0.5818 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1736 | 1492 | 1972 | 1228 | 1234 | 87 | 1647 | 88 | 2970 | 1579 | 3619 | 1316 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 643.26 | 668.22 | 798.10 | 817.74 | 724.23 | 801.65 | 845.92 | 884.10 | 676.90 | 675.57 | 819.86 | 822.18 |
Standard Deviation | 80.86 | 119.16 | 93.33 | 121.36 | 90.06 | 94.95 | 100.56 | 125.05 | 93.72 | 121.82 | 99.57 | 122.73 |
Intensity Change Log 2 | — | 0.054921 | 0.311168 | 0.346240 | — | 0.146525 | 0.224073 | 0.287762 | — | 0.104158 | 0.265698 | 0.315565 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000066 | 0.001725 | 0.000639 | 0.009986 | 0.000113 | 0.000554 | 0.000134 | 0.013926 | 0.000086 | 0.001660 | 0.000409 | 0.010249 |
Bud Neck | 0.037431 | 0.048326 | 0.013369 | 0.031004 | 0.015480 | 0.044928 | 0.014522 | 0.028967 | 0.028311 | 0.048139 | 0.013894 | 0.030868 |
Bud Site | 0.011996 | 0.101861* | 0.024743 | 0.154987* | 0.005473 | 0.027773 | 0.014005 | 0.210175* | 0.009286 | 0.097779 | 0.019856 | 0.158677* |
Cell Periphery | 0.000175 | 0.024040 | 0.000183 | 0.001290 | 0.000178 | 0.014626 | 0.000150 | 0.003921 | 0.000176 | 0.023522 | 0.000168 | 0.001466 |
Cytoplasm | 0.323342* | 0.356846* | 0.391609* | 0.354866* | 0.076696 | 0.064734 | 0.456592* | 0.317536* | 0.220864* | 0.340751* | 0.421183* | 0.352369* |
Cytoplasmic Foci | 0.114838 | 0.070379 | 0.022215 | 0.046648 | 0.024923 | 0.067641 | 0.028959 | 0.073492 | 0.077479 | 0.070228 | 0.025284 | 0.048443 |
Eisosomes | 0.000128 | 0.000142 | 0.000048 | 0.000192 | 0.000025 | 0.000115 | 0.000041 | 0.000393 | 0.000085 | 0.000141 | 0.000045 | 0.000205 |
Endoplasmic Reticulum | 0.001054 | 0.004466 | 0.003059 | 0.001676 | 0.001603 | 0.001827 | 0.001585 | 0.002636 | 0.001282 | 0.004321 | 0.002388 | 0.001740 |
Endosome | 0.070131 | 0.047164 | 0.008944 | 0.034749 | 0.047501 | 0.135613 | 0.021572 | 0.040634 | 0.060729 | 0.052037 | 0.014691 | 0.035142 |
Golgi | 0.001658 | 0.002658 | 0.000301 | 0.004987 | 0.000480 | 0.007675 | 0.000825 | 0.001226 | 0.001168 | 0.002934 | 0.000539 | 0.004736 |
Lipid Particles | 0.006181 | 0.002605 | 0.001459 | 0.002371 | 0.001457 | 0.003952 | 0.001578 | 0.004354 | 0.004218 | 0.002679 | 0.001513 | 0.002504 |
Mitochondria | 0.002512 | 0.003319 | 0.000830 | 0.001262 | 0.000907 | 0.006343 | 0.000279 | 0.000193 | 0.001845 | 0.003485 | 0.000579 | 0.001190 |
Mitotic Spindle | 0.001581 | 0.001322 | 0.005896 | 0.021164 | 0.000221 | 0.000736 | 0.002504 | 0.015279 | 0.001016 | 0.001290 | 0.004353 | 0.020770 |
None | 0.003031 | 0.003814 | 0.002862 | 0.003732 | 0.000649 | 0.000036 | 0.003248 | 0.000507 | 0.002042 | 0.003606 | 0.003038 | 0.003517 |
Nuclear Periphery | 0.000357 | 0.000219 | 0.003395 | 0.001076 | 0.000303 | 0.000399 | 0.001734 | 0.000383 | 0.000335 | 0.000229 | 0.002639 | 0.001029 |
Nuclear Periphery Foci | 0.000468 | 0.000292 | 0.001512 | 0.002023 | 0.000327 | 0.000285 | 0.001887 | 0.006136 | 0.000410 | 0.000291 | 0.001683 | 0.002298 |
Nucleolus | 0.001986 | 0.001378 | 0.001511 | 0.001379 | 0.000813 | 0.000690 | 0.002230 | 0.000411 | 0.001499 | 0.001340 | 0.001838 | 0.001314 |
Nucleus | 0.057884 | 0.027580 | 0.391403* | 0.053773 | 0.024888 | 0.022115 | 0.215930* | 0.027522 | 0.044175 | 0.027279 | 0.311546* | 0.052018 |
Peroxisomes | 0.003722 | 0.004129 | 0.001135 | 0.007706 | 0.001032 | 0.005350 | 0.000892 | 0.005153 | 0.002604 | 0.004197 | 0.001024 | 0.007536 |
Vacuole | 0.358350* | 0.289433* | 0.124330 | 0.261374* | 0.786800* | 0.586936* | 0.230687* | 0.246521* | 0.536366* | 0.305825* | 0.172733 | 0.260381* |
Vacuole Periphery | 0.003109 | 0.008303 | 0.000557 | 0.003756 | 0.010129 | 0.007673 | 0.000645 | 0.000633 | 0.006025 | 0.008268 | 0.000597 | 0.003547 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.69 | -2.48 | -4.30 | -2.65 | -3.82 | -1.65 | -0.14 | -1.75 | -1.62 | -1.75 | -5.66 | -2.39 | -4.57 | -2.93 | -4.30 |
Bud Neck | -2.87 | 9.56 | 5.51 | 7.50 | -3.45 | -2.50 | 1.21 | -0.36 | 2.25 | -0.90 | -6.24 | 8.86 | 2.84 | 7.85 | -4.22 |
Bud Site | -18.41 | -2.77 | -12.02 | 3.26 | -10.47 | -2.43 | -2.91 | -5.04 | -3.53 | -4.82 | -19.32 | -3.59 | -13.49 | 2.54 | -12.31 |
Cell Periphery | -9.44 | 1.29 | -4.60 | 9.26 | -4.83 | -1.70 | 2.38 | -1.62 | 1.52 | -1.70 | -9.59 | 2.62 | -4.68 | 9.37 | -5.10 |
Cytoplasm | -3.63 | -2.60 | 0.58 | 3.85 | 2.91 | 0.98 | -41.82 | -6.24 | -6.19 | 6.29 | -14.06 | -24.27 | -10.47 | 2.87 | 7.60 |
Cytoplasmic Foci | 10.05 | 27.18 | 20.28 | 9.23 | -8.82 | -3.32 | -1.31 | -3.73 | 0.46 | -3.46 | 2.06 | 22.57 | 13.18 | 8.93 | -8.21 |
Eisosomes | -0.69 | 8.92 | 1.09 | 1.46 | -4.70 | -4.57 | -5.86 | -1.60 | -0.74 | -1.45 | -3.96 | 7.56 | -2.17 | 1.06 | -5.11 |
Endoplasmic Reticulum | -4.18 | -17.03 | -6.83 | 2.91 | 10.98 | -0.50 | -2.39 | -1.88 | -1.13 | -1.25 | -3.93 | -15.45 | -6.14 | 2.85 | 7.21 |
Endosome | 6.51 | 21.31 | 13.54 | 7.54 | -6.71 | -4.25 | 10.14 | 1.71 | 4.60 | -0.88 | 2.94 | 22.78 | 13.12 | 8.60 | -4.89 |
Golgi | -1.58 | 3.55 | -0.68 | 0.41 | -2.66 | -1.24 | -0.06 | -0.79 | 1.21 | -0.35 | -2.96 | 2.81 | -1.30 | 0.85 | -2.46 |
Lipid Particles | 7.78 | 11.75 | 8.75 | 0.08 | -4.48 | -2.96 | -0.45 | -3.69 | 0.31 | -3.74 | 4.48 | 10.53 | 5.50 | 0.09 | -5.46 |
Mitochondria | -0.99 | 3.02 | 2.93 | 4.00 | 0.06 | -0.98 | 2.21 | 2.61 | 1.10 | 2.20 | -2.29 | 3.66 | 2.75 | 4.05 | -1.52 |
Mitotic Spindle | 0.26 | -5.04 | -6.28 | -6.41 | -3.16 | -0.72 | -3.80 | -1.74 | -1.70 | -1.40 | -0.38 | -6.10 | -6.83 | -6.63 | -4.38 |
None | -0.58 | 0.11 | 0.06 | 0.60 | -0.02 | 1.76 | -4.96 | -0.74 | -3.31 | 4.69 | -1.45 | -2.12 | -0.99 | 0.58 | 0.18 |
Nuclear Periphery | 1.40 | -20.69 | -13.22 | -14.37 | 9.92 | -0.82 | -15.50 | -4.96 | -3.77 | 7.12 | 1.34 | -25.19 | -14.43 | -14.59 | 8.01 |
Nuclear Periphery Foci | 1.89 | -5.48 | -8.63 | -10.21 | -0.56 | 0.31 | -7.01 | -4.78 | -4.91 | -2.83 | 1.34 | -8.46 | -9.79 | -11.18 | -1.52 |
Nucleolus | 2.29 | -1.85 | 0.28 | -1.87 | 2.04 | 0.57 | -4.43 | 0.23 | -0.19 | 3.93 | 0.99 | -3.72 | -1.12 | -1.84 | 2.07 |
Nucleus | 9.81 | -51.10 | -8.23 | -15.06 | 41.44 | 0.45 | -34.09 | -3.03 | -2.99 | 11.47 | 6.46 | -57.72 | -11.83 | -15.30 | 39.17 |
Peroxisomes | -0.38 | 5.94 | -0.87 | -0.70 | -4.13 | -2.50 | 1.08 | -2.31 | -0.08 | -2.44 | -3.56 | 6.05 | -2.41 | -0.72 | -4.63 |
Vacuole | 6.55 | 26.62 | -1.98 | -7.57 | -23.77 | 5.48 | 52.51* | 9.08 | 2.95 | -4.25 | 23.41 | 45.01* | 14.29 | -6.53 | -19.16 |
Vacuole Periphery | -8.15 | 14.51 | 2.09 | 7.57 | -2.21 | 1.62 | 13.46 | 13.51 | 5.50 | 0.92 | -3.17 | 17.13 | 6.81 | 8.12 | -2.21 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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