Standard name
Human Ortholog
Description Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.97 0.99 0.97 0.99 0.9 0.89 – 0.78 0.75 0.72 0.68 0.98 0.99 0.98 0.98 1.0 0.98 0.82 0.9 0.85 0.89 0.88 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.06 0 0.12 0.06 – 0.11 0.2 0.36 0.3 0 0 0 0 0 0 0.07 0 0.05 0 0 0.05
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.1 – 0.15 0.1 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 0 0 1 0 0 1 0 0 0 2 0 2 2 4 4
Bud 1 0 1 2 0 2 1 – 3 2 3 8 0 1 1 0 0 0 3 0 0 0 5 8
Bud Neck 0 0 2 1 1 0 1 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 1 1 6 5 2 7 – 9 12 7 15 1 1 2 6 3 3 0 0 1 1 2 1
Cytoplasm 231 83 172 231 291 246 359 – 155 228 219 250 319 366 389 163 136 118 205 87 154 147 318 251
Endoplasmic Reticulum 2 1 1 0 0 1 0 – 1 3 2 4 1 0 0 6 11 8 2 0 0 2 0 3
Endosome 1 0 0 0 1 0 3 – 1 0 0 0 0 0 1 1 0 0 5 3 1 0 0 1
Golgi 0 0 1 0 0 0 0 – 0 1 0 0 0 0 0 0 0 1 0 0 1 0 1 0
Mitochondria 10 4 0 14 1 32 26 – 22 60 110 108 3 0 1 6 3 1 17 1 9 4 15 13
Nucleus 0 0 0 1 0 4 2 – 4 6 5 4 1 1 2 2 0 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 1 0 – 1 1 0 8 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 6 – 4 0 1 0 1 0 0 0 0 0 3 0 2 0 4 7
Vac/Vac Membrane 4 0 0 4 2 4 39 – 29 31 27 40 1 2 2 4 1 0 2 0 0 0 2 1
Unique Cell Count 242 86 174 237 295 273 405 200 302 303 366 326 369 397 167 136 120 250 97 182 165 362 305
Labelled Cell Count 255 90 178 259 304 292 444 230 344 374 439 327 371 399 188 154 133 250 97 182 165 362 305


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.9 5.6 5.3 6.4 4.4 6.0 – 7.3 6.5 6.2 6.7 5.3 6.4 7.6 6.5 6.6 7.1 4.4 4.4 4.7
Std Deviation (1e-4) 1.6 0.8 2.0 2.0 2.7 1.6 2.5 – 5.3 3.0 2.6 5.3 1.7 3.8 4.8 1.7 2.1 2.2 1.7 1.5 1.9
Intensity Change (Log2) – – – -0.08 0.2 -0.35 0.1 – 0.38 0.22 0.15 0.26 -0.07 0.2 0.46 0.21 0.24 0.34 -0.33 -0.33 -0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4022 0.5058 0.0492 -0.3903 0.2714 0.4818 0.994 2.7003 2.0873 1.7121 1.0679 2.3632 2.7082 4.3718 3.5732 3.6378 4.3208 3.7093
Actin 0.0534 0.0004 0.0171 0.0018 0.0631 0.0047 0.0194 0.0008 0.0029 0.0049 0.0005 0.0025 0.023 0.0018 0.0041 0.0006 0.0293 0.0008
Bud 0.0007 0.0066 0.0023 0.0002 0.0017 0.0033 0.0011 0.0013 0.0028 0.0019 0.001 0.0013 0.0014 0.0045 0.0026 0.0002 0.0009 0.0003
Bud Neck 0.0019 0.0001 0.0021 0.0001 0.0006 0.0019 0.0021 0.0002 0.0003 0.0087 0.0318 0.004 0.0023 0.0004 0.0002 0.0003 0.0008 0.0005
Bud Periphery 0.0013 0.0037 0.0038 0.0002 0.0021 0.0076 0.0016 0.0011 0.0016 0.0018 0.0003 0.0012 0.0015 0.0035 0.0037 0.0002 0.0006 0.0003
Bud Site 0.0026 0.0007 0.013 0.0002 0.0004 0.0004 0.003 0.003 0.0014 0.0025 0.0009 0.0027 0.002 0.0059 0.001 0.0001 0.0013 0.0004
Cell Periphery 0.0003 0.0002 0.0004 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0005 0.0005 0.0002 0 0.0001 0
Cytoplasm 0.3278 0.3979 0.3902 0.2774 0.4578 0.4315 0.5897 0.7571 0.6967 0.7551 0.7413 0.8009 0.431 0.5979 0.5431 0.5507 0.5781 0.5588
Cytoplasmic Foci 0.0385 0.0053 0.021 0.0267 0.0082 0.0111 0.0246 0.0149 0.0127 0.0266 0.0109 0.0125 0.0149 0.004 0.0161 0.0117 0.0116 0.005
Eisosomes 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0001 0 0.0001 0.0008 0.0001 0.0001 0 0.0007 0
Endoplasmic Reticulum 0.0076 0.0011 0.0019 0.0017 0.0015 0.0013 0.0066 0.0025 0.0029 0.0033 0.0037 0.0036 0.0028 0.0014 0.0023 0.0028 0.0023 0.001
Endosome 0.0332 0.0011 0.0072 0.0187 0.0042 0.0055 0.0192 0.0039 0.0058 0.0192 0.0057 0.0094 0.0036 0.0017 0.0025 0.0108 0.0081 0.0018
Golgi 0.0101 0.0001 0.0034 0.0009 0.0068 0.0044 0.0033 0.0003 0.0007 0.0033 0.0002 0.001 0.0012 0.0004 0.0007 0.0002 0.0017 0.0003
Lipid Particles 0.0176 0.0002 0.0042 0.0007 0.0002 0.0019 0.0057 0.0001 0.0007 0.0017 0.0001 0.0022 0.0008 0.0002 0.0002 0.0001 0.0029 0.0006
Mitochondria 0.0054 0.0001 0.0015 0.0014 0.0034 0.0074 0.0027 0.0003 0.0008 0.0022 0.0004 0.0016 0.0022 0.0039 0.0004 0.0003 0.0021 0.0004
None 0.4432 0.5798 0.504 0.6581 0.4435 0.5117 0.2889 0.2086 0.2528 0.1359 0.177 0.1339 0.5041 0.3654 0.4161 0.4159 0.3266 0.4249
Nuclear Periphery 0.0133 0.0004 0.0013 0.0009 0.0007 0.001 0.0078 0.0009 0.0032 0.0021 0.0015 0.0068 0.0011 0.0007 0.0007 0.0013 0.0222 0.0009
Nucleolus 0.0007 0.0001 0.0037 0.0002 0.0001 0.0001 0.0008 0.0001 0.0002 0.0005 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0004 0.0004
Nucleus 0.0065 0.0009 0.0026 0.0015 0.0023 0.0017 0.0063 0.0025 0.0062 0.0076 0.0193 0.0063 0.0023 0.0039 0.0013 0.0025 0.003 0.0014
Peroxisomes 0.0219 0.0001 0.0122 0.0047 0.0003 0.0022 0.0063 0.0004 0.0007 0.0069 0.0001 0.0039 0.0011 0.0004 0.003 0.0001 0.0016 0.0003
Punctate Nuclear 0.0103 0.0004 0.0044 0.0014 0.0006 0.0005 0.0044 0.0008 0.0057 0.0089 0.0024 0.0034 0.0013 0.0004 0.0006 0.0008 0.0034 0.0013
Vacuole 0.0022 0.0007 0.0033 0.0024 0.0019 0.0011 0.0047 0.001 0.0014 0.0059 0.0023 0.0016 0.0017 0.0026 0.0009 0.0011 0.0011 0.0005
Vacuole Periphery 0.0011 0.0001 0.0003 0.0007 0.0005 0.0005 0.0013 0.0001 0.0003 0.0008 0.0003 0.0007 0.0002 0.0003 0.0001 0.0003 0.0012 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.3377 23.3659 12.3847 19.2056 14.524 20.5258 24.469 22.2098 26.1593 19.7527
Translational Efficiency 1.167 1.0711 1.965 1.6526 1.8337 1.8705 1.3466 1.2054 1.5806 1.7132

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
242 746 1712 1845 1568 1249 1787 1404 1810 1995 3499 3249

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 624.23 727.41 1137.07 832.93 650.55 757.97 968.19 950.27 647.03 746.54 1050.82 883.64
Standard Deviation 149.49 108.27 343.45 129.62 78.92 88.33 256.98 310.59 92.00 97.40 313.96 233.68
Intensity Change Log 2 — 0.220691 0.865171 0.416118 — 0.220481 0.573630 0.546678 — 0.220584 0.723750 0.484220

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000175 0.000492 0.000215 0.001683 0.000253 0.000166 0.000165 0.001118 0.000243 0.000288 0.000190 0.001439
Bud Neck 0.036553 0.028365 0.003139 0.005325 0.011838 0.026246 0.002199 0.003888 0.015143 0.027038 0.002659 0.004704
Bud Site 0.007635 0.015003 0.003666 0.023948 0.003288 0.011723 0.005893 0.021155 0.003869 0.012950 0.004804 0.022741
Cell Periphery 0.000648 0.000167 0.000119 0.000143 0.000131 0.000210 0.000099 0.000344 0.000200 0.000194 0.000109 0.000230
Cytoplasm 0.319684* 0.399593* 0.582351* 0.600142* 0.509699* 0.504928* 0.567444* 0.672675* 0.484293* 0.465540* 0.574738* 0.631486*
Cytoplasmic Foci 0.305881* 0.239526* 0.005392 0.022502 0.153832 0.123754 0.009365 0.023849 0.174161 0.167045 0.007421 0.023084
Eisosomes 0.000469 0.000130 0.000007 0.000035 0.000090 0.000072 0.000017 0.000035 0.000141 0.000094 0.000012 0.000035
Endoplasmic Reticulum 0.000867 0.001668 0.000987 0.001275 0.000631 0.000706 0.001911 0.001117 0.000662 0.001066 0.001459 0.001207
Endosome 0.013879 0.026075 0.000460 0.006629 0.005441 0.007660 0.001121 0.003727 0.006569 0.014546 0.000798 0.005375
Golgi 0.005285 0.003735 0.000157 0.004592 0.000870 0.001461 0.000075 0.002268 0.001461 0.002312 0.000115 0.003588
Lipid Particles 0.021069 0.008655 0.000371 0.000919 0.004092 0.003993 0.000599 0.000939 0.006362 0.005736 0.000487 0.000928
Mitochondria 0.038535 0.008091 0.000589 0.001370 0.002742 0.008081 0.000383 0.002898 0.007527 0.008085 0.000483 0.002030
Mitotic Spindle 0.000136 0.004582 0.008489 0.021346 0.000769 0.005411 0.012047 0.021969 0.000685 0.005101 0.010306 0.021615
None 0.008365 0.004189 0.010943 0.006354 0.004187 0.003536 0.008009 0.006909 0.004746 0.003780 0.009445 0.006594
Nuclear Periphery 0.000403 0.000660 0.000690 0.001022 0.000426 0.000658 0.001248 0.000727 0.000423 0.000659 0.000975 0.000894
Nuclear Periphery Foci 0.000376 0.000715 0.001037 0.000780 0.000322 0.000155 0.001427 0.001521 0.000329 0.000365 0.001236 0.001100
Nucleolus 0.001295 0.002153 0.000216 0.000309 0.000864 0.001926 0.000188 0.000409 0.000922 0.002011 0.000202 0.000352
Nucleus 0.188001* 0.154662* 0.362618* 0.198261* 0.268211* 0.247278* 0.344589* 0.128611 0.257487* 0.212645* 0.353411* 0.168163*
Peroxisomes 0.027207 0.015456 0.000057 0.001865 0.002874 0.004425 0.000085 0.002025 0.006127 0.008550 0.000071 0.001934
Vacuole 0.021468 0.083229 0.018377 0.099123 0.028771 0.045661 0.043060 0.100007 0.027794 0.059709 0.030983 0.099505
Vacuole Periphery 0.002069 0.002853 0.000119 0.002377 0.000670 0.001951 0.000075 0.003809 0.000857 0.002288 0.000097 0.002996

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.65 -2.95 -2.64 -1.31 -2.13 0.70 0.12 -1.81 -2.86 -2.45 -0.43 -0.33 -2.66 -2.72 -2.93
Bud Neck 1.17 4.99 4.89 9.05 -1.19 -6.79 8.95 8.51 11.70 -0.60 -6.13 9.44 8.96 14.63 -1.57
Bud Site -3.06 1.51 -2.80 0.27 -5.86 -5.22 -1.86 -6.40 -0.63 -4.60 -7.40 -1.65 -8.41 -0.53 -7.30
Cell Periphery 2.19 2.24 2.48 4.89 4.32 -2.91 2.01 -0.89 0.24 -1.18 0.05 2.20 1.25 1.43 -0.36
Cytoplasm -4.00 -12.59 -11.34 -10.06 2.31 0.47 2.39 -6.18 -6.21 -8.70 2.14 -3.65 -7.10 -9.65 -4.33
Cytoplasmic Foci 3.90 19.48 18.73 29.38 -10.70 4.69 31.47 28.71 22.40 -8.79 1.18 35.93 33.35 35.05 -13.50
Eisosomes 3.33 4.54 4.27 12.65 -19.07 3.16 13.70 10.94 8.41 -5.40 3.17 8.57 7.23 14.87 -15.51
Endoplasmic Reticulum -2.42 -2.26 -2.34 1.05 -0.01 -0.39 -12.35 -4.49 -3.05 7.69 -2.78 -12.44 -7.13 -1.74 6.41
Endosome -4.06 5.84 4.37 10.97 -5.46 -2.80 7.47 5.26 8.81 -3.00 -7.82 9.81 5.39 12.80 -6.03
Golgi 1.43 5.55 2.90 1.81 -3.02 -1.50 5.37 0.05 1.25 -2.34 -2.21 7.37 -0.20 1.49 -3.69
Lipid Particles 3.93 6.63 6.53 9.80 -5.03 0.29 10.97 10.44 8.42 -1.26 1.08 11.20 10.78 12.82 -4.13
Mitochondria 3.44 4.30 4.26 5.78 -1.71 -4.06 4.61 2.24 5.25 -1.55 -0.32 5.44 4.95 7.61 -2.25
Mitotic Spindle -3.06 -7.62 -8.37 -5.01 -2.39 -3.69 -7.15 -7.54 -4.78 -1.11 -4.56 -9.98 -10.97 -6.91 -2.40
None 1.02 -0.36 0.65 -1.37 5.27 0.76 -4.97 -1.89 -3.00 3.53 1.07 -5.03 -1.05 -2.92 5.96
Nuclear Periphery -3.62 -9.94 -16.25 -11.76 -9.45 -5.37 -23.64 -13.24 -7.23 9.58 -6.44 -25.38 -21.85 -14.37 -0.61
Nuclear Periphery Foci -1.85 -6.04 -2.58 -0.64 5.54 1.64 -10.02 -7.68 -12.37 2.73 -0.30 -11.16 -6.86 -10.01 6.23
Nucleolus -2.89 4.59 4.21 8.62 -2.99 -3.34 6.74 4.75 5.07 -1.53 -4.91 7.88 6.47 7.97 -2.85
Nucleus 2.08 -11.84 -2.79 -8.78 17.65 2.26 -12.13 14.61 11.49 28.88 5.76 -16.10 8.57 2.32 31.14
Peroxisomes 2.43 6.07 5.86 8.20 -2.56 -2.09 5.48 3.38 5.56 -2.31 -2.31 7.90 6.53 9.78 -3.42
Vacuole -10.94 -4.06 -24.63 -12.47 -24.03 -5.60 -14.39 -26.07 -22.61 -15.51 -11.56 -13.11 -36.80 -26.59 -26.59
Vacuole Periphery -1.38 4.68 1.48 2.84 -1.97 -3.66 4.67 -1.02 0.71 -1.87 -5.26 6.13 -0.87 2.16 -2.72
Standard name
Human Ortholog
Description Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (48%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tsa2

Tsa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tsa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available