Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 5 | 6 | 13 | 13 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 1 | 5 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 5 | 2 | 6 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 3 | 1 | 0 | 6 | 11 | 15 | 9 | 20 | 8 | 11 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 202 | 82 | 243 | 200 | 257 | 201 | 223 | 243 | 207 | 249 | 125 | 188 | 143 | 172 | 172 | 135 | 108 | 90 | 189 | 74 | 215 | 122 | 147 | 196 |
Endoplasmic Reticulum | 4 | 3 | 6 | 2 | 8 | 7 | 18 | 45 | 40 | 51 | 27 | 30 | 4 | 2 | 1 | 4 | 5 | 1 | 1 | 2 | 9 | 1 | 2 | 3 |
Endosome | 0 | 4 | 5 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 8 | 4 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 1 |
Golgi | 1 | 0 | 3 | 0 | 1 | 0 | 4 | 5 | 13 | 22 | 8 | 10 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 0 | 2 | 1 | 3 | 2 | 17 | 15 | 11 | 10 | 0 | 2 | 0 | 4 | 1 | 0 | 0 | 3 | 0 | 2 | 0 | 1 |
Nucleus | 0 | 7 | 5 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 2 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 13 | 3 | 11 | 2 | 4 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 0 | 3 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 6 | 2 | 4 | 1 |
Vac/Vac Membrane | 0 | 20 | 11 | 10 | 5 | 3 | 3 | 10 | 7 | 19 | 7 | 6 | 12 | 12 | 2 | 4 | 1 | 2 | 3 | 5 | 6 | 4 | 3 | 3 |
Unique Cell Count | 207 | 115 | 269 | 214 | 266 | 214 | 255 | 307 | 278 | 361 | 183 | 259 | 168 | 187 | 176 | 138 | 110 | 92 | 199 | 118 | 260 | 155 | 177 | 221 |
Labelled Cell Count | 210 | 123 | 277 | 217 | 275 | 221 | 267 | 329 | 306 | 396 | 209 | 278 | 176 | 198 | 180 | 147 | 115 | 94 | 199 | 118 | 260 | 155 | 177 | 221 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 101.4 | 47.2 | 81.4 | 75.6 | 61.0 | 45.4 | 38.1 | 33.9 | 29.2 | 30.1 | 25.6 | 26.9 | 71.5 | 71.0 | 65.0 | 81.1 | 105.5 | 114.8 | 71.8 | 75.5 | 72.0 |
Std Deviation (1e-4) | 20.6 | 33.5 | 32.4 | 30.3 | 23.3 | 17.1 | 14.1 | 10.6 | 11.5 | 10.9 | 12.3 | 9.9 | 37.8 | 37.7 | 34.6 | 25.6 | 31.5 | 43.7 | 41.8 | 48.3 | 39.7 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.42 | -0.84 | -1.1 | -1.26 | -1.48 | -1.44 | -1.67 | -1.6 | -0.19 | -0.2 | -0.32 | -0.01 | 0.37 | 0.5 | -0.18 | -0.11 | -0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 4.4 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 2.3 | 2.6 | 1.7 | 2.9 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.2 | 2.9 | 1.4 | -1.1 | -3.7 | -4.9 | -6.4 | -5.9 | -5.3 | -1.7 | 0.6 | 3.0 | 2.8 | 2.7 | 2.3 |
Endoplasmic Reticulum | 0 | 0.6 | 0.7 | 2.6 | 5.2 | 5.1 | 5.1 | 5.0 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 2.5 | 3.2 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.1 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.3 | -1.5 | -1.7 | -2.1 | -0.5 | -1.0 | 0.7 | -0.1 | -1.2 | 1.4 | 1.1 | -1.8 | -0.6 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 99.7089 | 121.0629 | 106.0613 | 106.5576 | 102.6441 | 105.9818 | 116.9865 | 138.1296 | 127.8264 | 133.985 | 100.0976 | 115.952 | 102.5116 | 129.1866 | 119.2302 | 107.6 | 104.895 | 115.4989 |
Actin | 0.0057 | 0.0024 | 0.0053 | 0.0005 | 0.0014 | 0.0046 | 0.0019 | 0.0003 | 0.0012 | 0.0002 | 0.0075 | 0.0019 | 0.0006 | 0.0004 | 0.0041 | 0.0224 | 0.0061 | 0.0013 |
Bud | 0.0013 | 0.0006 | 0.0014 | 0.0017 | 0.0006 | 0.0023 | 0.001 | 0.0007 | 0.0009 | 0.0007 | 0.0013 | 0.0015 | 0.0011 | 0.0002 | 0.0009 | 0.0019 | 0.0003 | 0.0004 |
Bud Neck | 0.0007 | 0.0006 | 0.001 | 0.0007 | 0.0005 | 0.0018 | 0.0005 | 0.0004 | 0.0005 | 0.0008 | 0.0012 | 0.0018 | 0.0004 | 0.0003 | 0.0007 | 0.0009 | 0.0006 | 0.0014 |
Bud Periphery | 0.0023 | 0.0005 | 0.0011 | 0.0008 | 0.0003 | 0.0017 | 0.0019 | 0.0006 | 0.0004 | 0.0003 | 0.0008 | 0.0005 | 0.0014 | 0.0001 | 0.0007 | 0.0014 | 0.0001 | 0.0005 |
Bud Site | 0.0009 | 0.0005 | 0.0014 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0.0004 | 0.0002 | 0.0009 | 0.0013 | 0.0004 | 0 |
Cell Periphery | 0.0225 | 0.0147 | 0.0117 | 0.0067 | 0.0026 | 0.0068 | 0.0122 | 0.0045 | 0.0037 | 0.0021 | 0.0023 | 0.0007 | 0.0041 | 0.001 | 0.0033 | 0.004 | 0.0005 | 0.0006 |
Cytoplasm | 0.6184 | 0.9012 | 0.7416 | 0.6524 | 0.6417 | 0.7151 | 0.81 | 0.9602 | 0.8877 | 0.8134 | 0.6997 | 0.9038 | 0.7689 | 0.9626 | 0.8297 | 0.6945 | 0.7064 | 0.9056 |
Cytoplasmic Foci | 0.018 | 0.0011 | 0.0171 | 0.0038 | 0.014 | 0.0041 | 0.006 | 0.0007 | 0.0067 | 0.0019 | 0.0544 | 0.0042 | 0.0087 | 0.0008 | 0.0101 | 0.0031 | 0.0123 | 0.0033 |
Eisosomes | 0.0005 | 0.0004 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0534 | 0.0122 | 0.0419 | 0.0038 | 0.0519 | 0.0498 | 0.0444 | 0.0057 | 0.0101 | 0.008 | 0.0048 | 0.0132 | 0.0576 | 0.012 | 0.0426 | 0.0048 | 0.0083 | 0.0347 |
Endosome | 0.0104 | 0.0032 | 0.0113 | 0.0066 | 0.0236 | 0.0306 | 0.0062 | 0.0013 | 0.0079 | 0.0088 | 0.0222 | 0.0208 | 0.012 | 0.0015 | 0.0127 | 0.0092 | 0.0553 | 0.012 |
Golgi | 0.0025 | 0.0017 | 0.0055 | 0.0004 | 0.0015 | 0.007 | 0.0015 | 0.0002 | 0.0019 | 0.0004 | 0.0045 | 0.0029 | 0.0012 | 0.0002 | 0.0044 | 0.0033 | 0.0051 | 0.0048 |
Lipid Particles | 0.0087 | 0.0007 | 0.0062 | 0.0014 | 0.0037 | 0.0021 | 0.0024 | 0.0003 | 0.002 | 0.0002 | 0.0108 | 0.0002 | 0.0022 | 0.0001 | 0.0017 | 0.001 | 0.0043 | 0 |
Mitochondria | 0.0164 | 0.0014 | 0.0099 | 0.0006 | 0.0097 | 0.0049 | 0.003 | 0.0003 | 0.0029 | 0.0005 | 0.0426 | 0.001 | 0.0098 | 0.0002 | 0.0076 | 0.0101 | 0.0024 | 0.0211 |
None | 0.0082 | 0.0003 | 0.0053 | 0.0011 | 0.0051 | 0.0008 | 0.003 | 0.0001 | 0.0085 | 0.0002 | 0.0386 | 0.0004 | 0.0027 | 0.0001 | 0.0013 | 0.0008 | 0.0061 | 0.0006 |
Nuclear Periphery | 0.0426 | 0.0064 | 0.0112 | 0.0111 | 0.0231 | 0.0349 | 0.0235 | 0.0026 | 0.0062 | 0.0117 | 0.0063 | 0.0047 | 0.0321 | 0.0039 | 0.0078 | 0.0178 | 0.0223 | 0.0041 |
Nucleolus | 0.0086 | 0.0002 | 0.0063 | 0.0013 | 0.031 | 0.0023 | 0.0006 | 0.0001 | 0.0012 | 0.0001 | 0.0077 | 0 | 0.002 | 0 | 0.0009 | 0.0005 | 0.0003 | 0 |
Nucleus | 0.0114 | 0.0022 | 0.006 | 0.0061 | 0.0076 | 0.0107 | 0.0063 | 0.0023 | 0.0027 | 0.0049 | 0.0053 | 0.0021 | 0.0084 | 0.0016 | 0.0033 | 0.0064 | 0.0063 | 0.0022 |
Peroxisomes | 0.0027 | 0 | 0.0018 | 0.0001 | 0.001 | 0.0002 | 0.0019 | 0 | 0.0031 | 0 | 0.0034 | 0.0001 | 0.0019 | 0 | 0.0009 | 0.0036 | 0.0014 | 0 |
Punctate Nuclear | 0.0049 | 0 | 0.0011 | 0.0001 | 0.0041 | 0.0009 | 0.0013 | 0 | 0.0003 | 0 | 0.0039 | 0.0001 | 0.001 | 0 | 0.0002 | 0.0001 | 0.0125 | 0 |
Vacuole | 0.1223 | 0.0447 | 0.0837 | 0.2475 | 0.122 | 0.0894 | 0.0593 | 0.0167 | 0.0443 | 0.1133 | 0.0491 | 0.031 | 0.0615 | 0.0131 | 0.0489 | 0.1549 | 0.1013 | 0.0049 |
Vacuole Periphery | 0.0375 | 0.005 | 0.0288 | 0.0529 | 0.0538 | 0.0297 | 0.0124 | 0.0025 | 0.0073 | 0.0325 | 0.0324 | 0.009 | 0.0219 | 0.0015 | 0.0171 | 0.0579 | 0.0476 | 0.0023 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 875.2064 | 1055.9745 | 1200.3 | 969.001 | 1129.5222 | 799.5924 | 818.9704 | 888.4941 | 910.0478 | 874.0853 |
Translational Efficiency | 1.8274 | 1.6683 | 1.5871 | 1.4676 | 1.6635 | 2.0 | 1.9395 | 1.7622 | 1.6704 | 1.9482 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
29 | 791 | 2916 | 2098 | 1805 | 1897 | 2063 | 2036 | 1834 | 2688 | 4979 | 4134 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3653.94 | 4623.21 | 10503.13 | 6589.87 | 4654.52 | 5872.21 | 8149.76 | 9115.55 | 4638.70 | 5504.67 | 9528.03 | 7833.77 |
Standard Deviation | 2045.69 | 1305.62 | 1831.46 | 2717.48 | 1176.73 | 1344.06 | 2227.69 | 2824.70 | 1201.90 | 1449.31 | 2316.17 | 3044.96 |
Intensity Change Log 2 | — | 0.339442 | 1.523294 | 0.850797 | — | 0.335271 | 0.808125 | 0.969697 | — | 0.337107 | 1.166746 | 0.918610 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000759 | 0.000101 | 0.000056 | 0.000164 | 0.000038 | 0.000088 | 0.000088 | 0.000106 | 0.000049 | 0.000092 | 0.000070 | 0.000135 |
Bud Neck | 0.006084 | 0.000763 | 0.000415 | 0.002980 | 0.000192 | 0.000197 | 0.001796 | 0.001346 | 0.000285 | 0.000364 | 0.000988 | 0.002176 |
Bud Site | 0.009125 | 0.004966 | 0.002375 | 0.009894 | 0.003103 | 0.002257 | 0.002895 | 0.005047 | 0.003198 | 0.003054 | 0.002590 | 0.007507 |
Cell Periphery | 0.036581 | 0.019300 | 0.018780 | 0.007772 | 0.008372 | 0.015744 | 0.008985 | 0.013062 | 0.008818 | 0.016791 | 0.014721 | 0.010377 |
Cytoplasm | 0.069148 | 0.574446* | 0.646940* | 0.673078* | 0.703066* | 0.681683* | 0.712953* | 0.678621* | 0.693042* | 0.650126* | 0.674292* | 0.675808* |
Cytoplasmic Foci | 0.026587 | 0.012008 | 0.007365 | 0.010829 | 0.010008 | 0.011185 | 0.004687 | 0.005936 | 0.010271 | 0.011427 | 0.006255 | 0.008419 |
Eisosomes | 0.000028 | 0.000002 | 0.000002 | 0.000002 | 0.000001 | 0.000001 | 0.000001 | 0.000002 | 0.000001 | 0.000002 | 0.000001 | 0.000002 |
Endoplasmic Reticulum | 0.007116 | 0.001240 | 0.002949 | 0.000387 | 0.001636 | 0.002167 | 0.000370 | 0.000563 | 0.001723 | 0.001894 | 0.001880 | 0.000474 |
Endosome | 0.012858 | 0.001276 | 0.000688 | 0.001199 | 0.000690 | 0.000852 | 0.000566 | 0.000750 | 0.000882 | 0.000977 | 0.000637 | 0.000978 |
Golgi | 0.112167* | 0.074097 | 0.032661 | 0.007047 | 0.052185 | 0.073922 | 0.015914 | 0.016525 | 0.053134 | 0.073973 | 0.025722 | 0.011715 |
Lipid Particles | 0.121095* | 0.022368 | 0.013447 | 0.009842 | 0.006775 | 0.014440 | 0.007801 | 0.011782 | 0.008583 | 0.016773 | 0.011108 | 0.010798 |
Mitochondria | 0.009202 | 0.000220 | 0.000379 | 0.000540 | 0.000327 | 0.000476 | 0.000286 | 0.000370 | 0.000467 | 0.000400 | 0.000341 | 0.000456 |
Mitotic Spindle | 0.016372 | 0.001207 | 0.000661 | 0.057810 | 0.000612 | 0.000746 | 0.001368 | 0.007379 | 0.000862 | 0.000882 | 0.000954 | 0.032972 |
None | 0.007727 | 0.005915 | 0.000953 | 0.005766 | 0.002092 | 0.003154 | 0.003238 | 0.002464 | 0.002181 | 0.003966 | 0.001900 | 0.004139 |
Nuclear Periphery | 0.003060 | 0.000574 | 0.000753 | 0.001170 | 0.000412 | 0.000486 | 0.001038 | 0.000689 | 0.000454 | 0.000512 | 0.000871 | 0.000933 |
Nuclear Periphery Foci | 0.052524 | 0.018409 | 0.009208 | 0.014134 | 0.010136 | 0.012002 | 0.010966 | 0.012346 | 0.010807 | 0.013887 | 0.009936 | 0.013253 |
Nucleolus | 0.009783 | 0.000174 | 0.000077 | 0.000611 | 0.000085 | 0.000094 | 0.000185 | 0.000207 | 0.000238 | 0.000118 | 0.000122 | 0.000412 |
Nucleus | 0.117386 | 0.009276 | 0.003744 | 0.045235 | 0.004695 | 0.003051 | 0.027659 | 0.018024 | 0.006477 | 0.004883 | 0.013653 | 0.031834 |
Peroxisomes | 0.001248 | 0.000040 | 0.000066 | 0.000087 | 0.000034 | 0.000036 | 0.000018 | 0.000039 | 0.000053 | 0.000037 | 0.000046 | 0.000063 |
Vacuole | 0.205486* | 0.157815 | 0.154557 | 0.134006 | 0.071754 | 0.078794 | 0.164598 | 0.187037 | 0.073869 | 0.102047 | 0.158718 | 0.160124 |
Vacuole Periphery | 0.175667* | 0.095803 | 0.103924* | 0.017448 | 0.123787* | 0.098627 | 0.034587 | 0.037706 | 0.124607* | 0.097796 | 0.075195 | 0.027425 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.54 | 2.72 | 2.14 | -4.99 | -8.31 | -1.99 | -6.71 | -5.53 | -1.83 | -0.80 | -2.44 | -5.03 | -9.41 | -3.75 | -6.11 |
Bud Neck | 1.89 | 1.99 | -1.23 | -15.43 | -16.07 | -0.29 | -10.75 | -10.49 | -10.47 | 0.61 | -1.56 | -10.26 | -19.04 | -18.86 | -13.36 |
Bud Site | 1.12 | 1.94 | -1.61 | -7.98 | -11.42 | 1.71 | -1.34 | -5.43 | -7.29 | -5.05 | 0.15 | 0.33 | -10.24 | -12.04 | -12.34 |
Cell Periphery | 2.00 | 2.07 | 3.72 | 8.32 | 14.77 | -7.33 | 0.27 | -3.43 | 3.65 | -3.40 | -8.44 | -7.30 | 0.79 | 9.48 | 8.67 |
Cytoplasm | -18.95 | -23.50 | -20.05 | 0.65 | 8.00 | 2.12 | 2.89 | 5.53 | 3.32 | 2.83 | 4.42 | 4.38 | 9.71 | 5.36 | 7.24 |
Cytoplasmic Foci | 1.86 | 2.48 | 0.24 | -5.94 | -9.17 | -1.32 | 4.10 | -1.18 | -0.23 | -4.19 | -1.39 | 4.32 | -6.20 | -5.57 | -9.93 |
Eisosomes | 2.44 | 2.50 | 2.32 | -4.01 | -7.50 | -2.77 | -7.33 | -6.73 | -2.41 | -0.38 | -1.76 | -2.63 | -6.54 | -5.30 | -6.15 |
Endoplasmic Reticulum | 2.90 | 2.05 | 3.39 | 4.41 | 9.97 | -1.70 | 6.23 | 5.36 | 6.67 | -2.83 | -0.66 | -0.54 | 6.43 | 8.06 | 9.26 |
Endosome | 2.79 | 2.93 | 2.69 | -2.36 | -6.53 | -2.81 | -0.85 | -3.80 | -1.40 | -3.16 | -0.97 | 1.99 | -3.84 | -3.86 | -7.29 |
Golgi | 1.62 | 3.38 | 4.54 | 18.51 | 26.93* | -6.57 | 16.86 | 16.52 | 22.12 | -0.59 | -6.92 | 12.51 | 19.73 | 29.66 | 20.45* |
Lipid Particles | 2.62 | 2.85 | 2.90 | 5.02 | 1.96 | -6.57 | -1.91 | -6.01 | 1.33 | -4.38 | -6.30 | -2.82 | -3.30 | 4.27 | -0.95 |
Mitochondria | 1.94 | 1.90 | 1.83 | -8.01 | -6.08 | -1.40 | -0.38 | -2.11 | -0.26 | -1.85 | 0.50 | 1.09 | -1.74 | -2.52 | -4.90 |
Mitotic Spindle | 2.12 | 2.19 | -9.31 | -19.15 | -19.27 | -2.03 | -4.51 | -7.50 | -7.44 | -6.66 | -0.12 | -2.09 | -20.02 | -20.03 | -19.88 |
None | 0.36 | 1.27 | 0.47 | 0.36 | -7.43 | -2.08 | -1.74 | -1.36 | 0.79 | 0.60 | -3.13 | 1.16 | -4.62 | -0.06 | -5.10 |
Nuclear Periphery | 3.40 | 3.09 | 1.16 | -10.31 | -9.13 | -2.15 | -8.67 | -7.46 | -6.63 | 1.50 | -1.58 | -9.89 | -13.07 | -12.65 | -6.73 |
Nuclear Periphery Foci | 2.10 | 2.59 | 2.05 | -0.43 | -6.74 | -2.38 | -1.88 | -4.87 | -3.23 | -3.11 | -3.51 | 0.47 | -6.25 | -3.48 | -7.44 |
Nucleolus | 2.16 | 2.19 | 1.66 | -5.73 | -5.99 | -0.63 | -8.74 | -6.78 | -6.08 | -2.51 | 1.55 | 1.12 | -5.50 | -6.45 | -6.29 |
Nucleus | 2.12 | 2.23 | 0.83 | -20.50 | -23.69 | 2.73 | -17.86 | -14.60 | -15.78 | 4.86 | 1.32 | -10.03 | -23.16 | -26.47 | -16.77 |
Peroxisomes | 1.88 | 1.84 | 1.63 | -6.20 | -5.11 | -0.23 | 2.47 | -1.89 | -1.73 | -2.80 | 1.36 | 0.51 | -4.23 | -6.25 | -5.53 |
Vacuole | 1.39 | 1.44 | 2.09 | 2.48 | 3.53 | -1.66 | -18.62 | -19.25 | -18.14 | -0.37 | -6.54 | -21.98 | -19.37 | -13.17 | 1.42 |
Vacuole Periphery | 2.03 | 1.80 | 4.25 | 16.66 | 35.27* | 4.68 | 20.84 | 20.42 | 18.33 | -0.95 | 5.42 | 11.40 | 24.05 | 26.38 | 27.71* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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