ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 7 | 5 | 9 | 18 | 11 | 9 | 9 | 14 | 14 | 14 | 26 | 8 | 26 | 39 | 5 | 3 | 3 | 4 | 8 | 1 | 9 | 24 | 23 |
Bud | 0 | 0 | 1 | 1 | 1 | 6 | 4 | 15 | 7 | 4 | 7 | 7 | 1 | 2 | 3 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 9 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 6 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 6 | 5 | 8 | 25 | 8 | 25 | 9 | 15 | 18 | 13 | 23 | 24 | 74 | 126 | 20 | 11 | 14 | 0 | 0 | 3 | 11 | 16 | 17 |
Cytoplasm | 28 | 42 | 22 | 44 | 79 | 52 | 69 | 102 | 66 | 56 | 63 | 60 | 39 | 53 | 60 | 56 | 65 | 35 | 4 | 11 | 7 | 21 | 24 | 27 |
Endoplasmic Reticulum | 121 | 148 | 60 | 120 | 181 | 80 | 158 | 61 | 108 | 113 | 74 | 144 | 255 | 446 | 448 | 9 | 7 | 2 | 136 | 167 | 99 | 346 | 469 | 439 |
Endosome | 1 | 0 | 1 | 2 | 3 | 1 | 3 | 7 | 2 | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 1 | 0 | 2 | 2 | 9 |
Golgi | 0 | 0 | 0 | 2 | 9 | 0 | 3 | 0 | 1 | 2 | 0 | 2 | 7 | 6 | 12 | 16 | 11 | 14 | 1 | 1 | 0 | 3 | 1 | 4 |
Mitochondria | 3 | 4 | 0 | 4 | 6 | 50 | 17 | 28 | 23 | 10 | 61 | 57 | 2 | 9 | 1 | 13 | 10 | 8 | 8 | 4 | 9 | 29 | 33 | 29 |
Nucleus | 0 | 3 | 2 | 0 | 2 | 3 | 5 | 11 | 5 | 2 | 5 | 6 | 1 | 1 | 2 | 4 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 3 | 2 | 0 | 0 | 1 | 2 | 3 | 0 | 4 | 2 | 3 | 11 | 9 | 5 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 12 | 11 | 4 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 17 | 7 | 26 | 44 | 127 | 69 | 216 | 285 | 87 | 118 | 91 | 147 | 35 | 44 | 49 | 45 | 46 | 34 | 1 | 4 | 4 | 20 | 31 | 36 |
Unique Cell Count | 156 | 191 | 100 | 194 | 364 | 230 | 420 | 469 | 274 | 280 | 274 | 400 | 326 | 548 | 593 | 128 | 129 | 81 | 163 | 209 | 130 | 470 | 641 | 613 |
Labelled Cell Count | 179 | 219 | 123 | 234 | 452 | 283 | 512 | 528 | 332 | 340 | 335 | 486 | 381 | 667 | 743 | 169 | 160 | 112 | 163 | 209 | 130 | 470 | 641 | 613 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 9.0 | 7.9 | 8.6 | 9.9 | 7.3 | 9.6 | 10.9 | 10.2 | 9.6 | 11.1 | 9.5 | 12.7 | 15.9 | 17.8 | 11.7 | 10.9 | 12.9 | 7.8 | 8.0 | 8.4 |
Std Deviation (1e-4) | 1.4 | 1.8 | 1.8 | 1.6 | 1.9 | 2.8 | 1.8 | 13.3 | 11.8 | 1.8 | 14.0 | 1.9 | 3.1 | 3.9 | 4.1 | 3.1 | 2.7 | 3.3 | 1.9 | 2.3 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.33 | -0.1 | 0.29 | 0.47 | 0.38 | 0.28 | 0.49 | 0.28 | 0.7 | 1.01 | 1.18 | 0.58 | 0.48 | 0.72 | -0.01 | 0.02 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0 | 0 | 0.6 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.7 | 0 | 0.4 | 0 | 0.2 | 0.5 | 0 | 0.3 | 0.8 | 2.4 | 3.8 | 2.5 | 1.0 | 2.7 |
Cytoplasm | 0.1 | -0.1 | 0.1 | -1.3 | -0.1 | 0.4 | -0.4 | 0.2 | -1.7 | -2.5 | -3.5 | -3.4 | 3.4 | 4.4 | 3.1 |
Endoplasmic Reticulum | 0.3 | -1.8 | -4.3 | -4.1 | -10.4 | -3.5 | -3.4 | -5.9 | -4.4 | 3.6 | 4.8 | 3.3 | -8.6 | -9.0 | -8.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 4.3 |
Mitochondria | 0 | 0 | 5.1 | 0 | 0 | 3.0 | 0 | 5.2 | 4.0 | 0 | 0 | 0 | 3.3 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.6 | 1.7 | 0.7 | 4.6 | 6.3 | 1.1 | 2.9 | 1.3 | 2.0 | -3.8 | -5.3 | -5.3 | 1.5 | 1.6 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.1187 | 6.9065 | 5.6286 | 5.7621 | 5.4991 | 5.9945 | 5.453 | 6.903 | 6.125 | 5.7358 | 5.1012 | 6.1772 | 8.3035 | 9.9845 | 8.6589 | 9.0243 | 8.0338 | 8.678 |
Actin | 0.0139 | 0.0004 | 0.0243 | 0.0029 | 0.0009 | 0.0007 | 0.0161 | 0.0002 | 0.0127 | 0.0128 | 0.0231 | 0.0007 | 0.0086 | 0.0008 | 0.0015 | 0.0002 | 0.0003 | 0.001 |
Bud | 0.0001 | 0 | 0.0007 | 0.0002 | 0 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0007 | 0.0001 | 0.0025 | 0.0106 | 0.0001 | 0.0006 | 0.0019 | 0.0001 | 0.0004 | 0.0003 | 0.002 | 0.0004 | 0.002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0007 |
Bud Periphery | 0.0001 | 0 | 0.0008 | 0.0002 | 0.0001 | 0.0006 | 0.0005 | 0 | 0.0002 | 0.0004 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 |
Bud Site | 0.0004 | 0.0002 | 0.0058 | 0.0032 | 0.0001 | 0.0004 | 0.0016 | 0.0001 | 0.001 | 0.0003 | 0.0035 | 0 | 0.0005 | 0.0018 | 0.0003 | 0 | 0 | 0 |
Cell Periphery | 0.0003 | 0.0027 | 0.002 | 0.0006 | 0.0001 | 0.0005 | 0.0005 | 0.0011 | 0.0021 | 0.0013 | 0.0004 | 0.0002 | 0.0006 | 0.0027 | 0.0016 | 0.0004 | 0.0004 | 0.0005 |
Cytoplasm | 0.0351 | 0.0405 | 0.0562 | 0.0907 | 0.0148 | 0.0314 | 0.0291 | 0.04 | 0.0388 | 0.0208 | 0.0543 | 0.0425 | 0.0292 | 0.1075 | 0.0537 | 0.0496 | 0.034 | 0.0372 |
Cytoplasmic Foci | 0.0052 | 0.0016 | 0.0038 | 0.0045 | 0.0011 | 0.0026 | 0.0161 | 0.0014 | 0.012 | 0.0031 | 0.019 | 0.0014 | 0.0011 | 0.0014 | 0.0014 | 0.0024 | 0.0008 | 0.0007 |
Eisosomes | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.8477 | 0.9026 | 0.8545 | 0.818 | 0.8984 | 0.9306 | 0.7413 | 0.9255 | 0.8759 | 0.9083 | 0.6867 | 0.907 | 0.9203 | 0.8605 | 0.9042 | 0.931 | 0.9431 | 0.9445 |
Endosome | 0.0193 | 0.0033 | 0.007 | 0.0137 | 0.0253 | 0.0029 | 0.0479 | 0.0055 | 0.0141 | 0.006 | 0.0585 | 0.0081 | 0.0052 | 0.0038 | 0.0049 | 0.0073 | 0.004 | 0.0023 |
Golgi | 0.0048 | 0.0004 | 0.0021 | 0.0052 | 0.0016 | 0.0002 | 0.0177 | 0.0005 | 0.0055 | 0.0011 | 0.0342 | 0.0014 | 0.001 | 0.0008 | 0.0014 | 0.0004 | 0.0004 | 0.0003 |
Lipid Particles | 0.0033 | 0.0139 | 0.0023 | 0.0291 | 0.0028 | 0.0015 | 0.0208 | 0.013 | 0.0099 | 0.0036 | 0.0341 | 0.008 | 0.0026 | 0.0044 | 0.0033 | 0.0022 | 0.0016 | 0.0016 |
Mitochondria | 0.0013 | 0.0001 | 0.001 | 0.0003 | 0.0018 | 0.0001 | 0.0064 | 0.0001 | 0.0039 | 0.0011 | 0.0343 | 0.0042 | 0.0004 | 0.0003 | 0.0011 | 0.0001 | 0.0001 | 0.0001 |
None | 0.0011 | 0.0012 | 0.0055 | 0.0003 | 0.0013 | 0.0028 | 0.0132 | 0.0004 | 0.0008 | 0.0158 | 0.0022 | 0.0004 | 0.0019 | 0.0017 | 0.0069 | 0.0008 | 0.0031 | 0.0007 |
Nuclear Periphery | 0.0502 | 0.0218 | 0.0223 | 0.0123 | 0.0398 | 0.0188 | 0.0464 | 0.0078 | 0.0083 | 0.0101 | 0.0137 | 0.0165 | 0.0216 | 0.0065 | 0.0144 | 0.0034 | 0.009 | 0.0071 |
Nucleolus | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0012 | 0 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0068 | 0.0017 | 0.0027 | 0.0029 | 0.0045 | 0.0026 | 0.0108 | 0.0005 | 0.0009 | 0.0019 | 0.0052 | 0.0018 | 0.0014 | 0.0011 | 0.0008 | 0.0003 | 0.0013 | 0.001 |
Peroxisomes | 0.0011 | 0 | 0.0003 | 0.0005 | 0.0002 | 0 | 0.01 | 0 | 0.0081 | 0.0006 | 0.0108 | 0.0007 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0026 | 0.0005 | 0.0018 | 0.0012 | 0.0013 | 0.001 | 0.0077 | 0.0001 | 0.0011 | 0.0058 | 0.0016 | 0.002 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
Vacuole | 0.0039 | 0.0084 | 0.003 | 0.0027 | 0.0033 | 0.0018 | 0.0074 | 0.0035 | 0.0032 | 0.0046 | 0.0109 | 0.0031 | 0.0021 | 0.0058 | 0.0027 | 0.0013 | 0.0014 | 0.0015 |
Vacuole Periphery | 0.0016 | 0.0007 | 0.0006 | 0.0005 | 0.0019 | 0.0004 | 0.0029 | 0.0002 | 0.0007 | 0.0015 | 0.0026 | 0.0012 | 0.0005 | 0.0003 | 0.0011 | 0.0001 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 73.5603 | 61.3745 | 94.3381 | 70.787 | 43.4482 | 102.9274 | 98.9936 | 103.7113 | 104.3689 | 75.3487 |
Translational Efficiency | 1.1342 | 1.2686 | 1.0862 | 1.6391 | 1.3285 | 1.0739 | 1.1933 | 1.7684 | 1.5629 | 1.34 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1624 | 385 | 207 | 2211 | 629 | 2166 | 2474 | 1238 | 2253 | 2551 | 2681 | 3449 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1021.94 | 999.02 | 1319.87 | 978.89 | 966.22 | 939.54 | 1166.24 | 1124.40 | 1006.38 | 948.52 | 1178.10 | 1031.12 |
Standard Deviation | 251.15 | 179.42 | 186.95 | 167.06 | 276.10 | 137.55 | 189.41 | 191.67 | 259.57 | 146.21 | 193.61 | 189.61 |
Intensity Change Log 2 | — | -0.032725 | 0.369085 | -0.062092 | — | -0.040397 | 0.271441 | 0.218732 | — | -0.036448 | 0.322456 | 0.081214 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001079 | 0.001429 | 0.005222 | 0.004824 | 0.000941 | 0.000677 | 0.001913 | 0.004188 | 0.001040 | 0.000790 | 0.002168 | 0.004595 |
Bud Neck | 0.001049 | 0.002802 | 0.003888 | 0.012212 | 0.001503 | 0.002955 | 0.002156 | 0.007036 | 0.001176 | 0.002932 | 0.002289 | 0.010354 |
Bud Site | 0.010721 | 0.011632 | 0.010126 | 0.038026 | 0.021067 | 0.004886 | 0.008889 | 0.036351 | 0.013609 | 0.005905 | 0.008985 | 0.037425 |
Cell Periphery | 0.005803 | 0.002502 | 0.003583 | 0.005074 | 0.006332 | 0.001784 | 0.003351 | 0.003937 | 0.005950 | 0.001893 | 0.003369 | 0.004666 |
Cytoplasm | 0.016965 | 0.081608 | 0.049323 | 0.222319* | 0.029357 | 0.136768* | 0.061809 | 0.129140* | 0.020425 | 0.128443* | 0.060845 | 0.188873* |
Cytoplasmic Foci | 0.011773 | 0.033657 | 0.007693 | 0.017611 | 0.033586 | 0.017921 | 0.001978 | 0.022323 | 0.017863 | 0.020296 | 0.002419 | 0.019303 |
Eisosomes | 0.000108 | 0.000296 | 0.000241 | 0.000941 | 0.000101 | 0.000672 | 0.000171 | 0.000473 | 0.000106 | 0.000615 | 0.000176 | 0.000773 |
Endoplasmic Reticulum | 0.915291* | 0.771182* | 0.723284* | 0.480298* | 0.777307* | 0.744911* | 0.875072* | 0.626269* | 0.876768* | 0.748876* | 0.863352* | 0.532694* |
Endosome | 0.006306 | 0.011064 | 0.016593 | 0.008129 | 0.021751 | 0.007071 | 0.003920 | 0.014119 | 0.010618 | 0.007674 | 0.004898 | 0.010279 |
Golgi | 0.005393 | 0.008808 | 0.001111 | 0.003265 | 0.013131 | 0.009042 | 0.000839 | 0.008488 | 0.007553 | 0.009007 | 0.000860 | 0.005139 |
Lipid Particles | 0.004903 | 0.018439 | 0.011914 | 0.018084 | 0.012636 | 0.019774 | 0.003463 | 0.016743 | 0.007062 | 0.019573 | 0.004116 | 0.017603 |
Mitochondria | 0.004150 | 0.003885 | 0.000295 | 0.003753 | 0.003563 | 0.006496 | 0.001028 | 0.002815 | 0.003986 | 0.006102 | 0.000972 | 0.003417 |
Mitotic Spindle | 0.000262 | 0.002912 | 0.001274 | 0.002322 | 0.000972 | 0.000574 | 0.000252 | 0.007250 | 0.000460 | 0.000927 | 0.000331 | 0.004091 |
None | 0.003170 | 0.004243 | 0.000104 | 0.003129 | 0.013479 | 0.007462 | 0.003072 | 0.001025 | 0.006048 | 0.006976 | 0.002843 | 0.002374 |
Nuclear Periphery | 0.000418 | 0.002542 | 0.031430 | 0.001836 | 0.007765 | 0.003468 | 0.000899 | 0.001291 | 0.002469 | 0.003329 | 0.003257 | 0.001640 |
Nuclear Periphery Foci | 0.004085 | 0.009750 | 0.087747 | 0.012814 | 0.027059 | 0.004738 | 0.006673 | 0.015090 | 0.010498 | 0.005495 | 0.012933 | 0.013631 |
Nucleolus | 0.000219 | 0.000128 | 0.000037 | 0.000270 | 0.001423 | 0.000185 | 0.000042 | 0.000230 | 0.000555 | 0.000176 | 0.000042 | 0.000256 |
Nucleus | 0.002056 | 0.008026 | 0.007538 | 0.081283 | 0.007461 | 0.017723 | 0.011935 | 0.023299 | 0.003565 | 0.016260 | 0.011596 | 0.060470 |
Peroxisomes | 0.001766 | 0.001726 | 0.000206 | 0.001268 | 0.001322 | 0.002550 | 0.000216 | 0.000983 | 0.001642 | 0.002425 | 0.000216 | 0.001166 |
Vacuole | 0.003051 | 0.020404 | 0.037861 | 0.081437 | 0.011196 | 0.008426 | 0.012114 | 0.071853 | 0.005325 | 0.010234 | 0.014102 | 0.077997 |
Vacuole Periphery | 0.001433 | 0.002965 | 0.000529 | 0.001105 | 0.008048 | 0.001917 | 0.000207 | 0.007098 | 0.003280 | 0.002075 | 0.000232 | 0.003256 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.80 | -1.68 | -7.96 | -5.66 | 0.19 | 1.22 | -2.19 | -6.23 | -6.89 | -3.67 | 1.39 | -2.55 | -10.00 | -10.90 | -4.42 |
Bud Neck | -1.98 | -2.64 | -12.12 | -8.90 | -5.42 | -2.22 | -1.49 | -8.88 | -5.61 | -7.34 | -2.78 | -2.33 | -14.29 | -10.38 | -12.36 |
Bud Site | -0.36 | -0.19 | -9.68 | -7.60 | -6.57 | 4.80 | 3.38 | -3.87 | -10.48 | -8.77 | 4.81 | 2.32 | -10.37 | -16.00 | -13.18 |
Cell Periphery | 2.52 | 0.86 | 0.95 | -2.50 | -0.42 | 3.21 | 2.06 | 1.63 | -4.13 | -0.99 | 4.86 | 2.93 | 1.70 | -7.52 | -2.64 |
Cytoplasm | -8.71 | -3.62 | -41.90 | -16.63 | -15.50 | -20.48 | -8.27 | -16.42 | -0.35 | -12.10 | -27.92 | -15.60 | -43.45 | -12.87 | -29.86 |
Cytoplasmic Foci | -4.80 | 0.93 | -4.26 | 3.29 | -3.59 | 3.99 | 8.42 | 2.34 | -2.67 | -11.61 | -1.32 | 10.55 | -1.64 | -0.22 | -18.11 |
Eisosomes | -3.01 | -1.35 | -10.70 | -6.74 | -5.47 | -3.96 | -1.67 | -5.74 | 1.34 | -5.11 | -4.22 | -2.37 | -12.10 | -1.06 | -10.52 |
Endoplasmic Reticulum | 8.41 | 8.89 | 47.44* | 17.38 | 10.52 | 2.07 | -6.33 | 10.04 | 11.26 | 22.27 | 15.31 | 2.60 | 42.50 | 26.66 | 42.88* |
Endosome | -3.58 | -4.86 | -2.45 | 2.34 | 4.09 | 6.73 | 8.44 | 3.30 | -5.84 | -9.57 | 3.14 | 7.08 | 0.47 | -3.27 | -8.71 |
Golgi | -2.19 | 5.80 | 2.58 | 3.85 | -3.08 | 1.82 | 5.76 | 1.98 | 0.62 | -4.87 | -1.40 | 8.40 | 2.69 | 4.52 | -5.99 |
Lipid Particles | -4.74 | -2.58 | -10.36 | 0.15 | -1.80 | -2.90 | 4.04 | -1.68 | 1.60 | -9.54 | -9.74 | 3.15 | -9.21 | 1.45 | -14.07 |
Mitochondria | 0.19 | 5.03 | 0.69 | 0.26 | -8.46 | -3.07 | 3.19 | 0.84 | 4.77 | -2.99 | -2.80 | 5.16 | 1.07 | 4.89 | -7.35 |
Mitotic Spindle | -1.05 | -2.50 | -3.48 | 0.22 | -1.06 | 1.03 | 2.12 | -3.90 | -4.15 | -4.36 | -1.03 | 0.50 | -5.23 | -3.97 | -5.34 |
None | -0.52 | 4.04 | 0.21 | 0.64 | -6.43 | 1.53 | 2.72 | 3.27 | 5.87 | 3.17 | -0.60 | 2.48 | 3.06 | 4.75 | 0.90 |
Nuclear Periphery | -2.87 | -5.41 | -6.45 | 0.86 | 5.15 | 2.26 | 3.74 | 3.52 | 3.81 | -2.70 | -1.21 | -1.09 | 1.47 | 3.28 | 3.17 |
Nuclear Periphery Foci | -3.65 | -8.64 | -8.49 | -1.99 | 7.69 | 5.59 | 5.02 | 2.73 | -8.11 | -6.20 | 3.93 | -1.66 | -2.47 | -10.48 | -0.72 |
Nucleolus | 1.07 | 2.08 | -0.78 | -4.50 | -9.36 | 2.78 | 3.10 | 2.60 | -0.70 | -2.44 | 2.72 | 3.71 | 1.99 | -2.50 | -6.84 |
Nucleus | -3.42 | -3.51 | -28.24 | -23.57 | -21.85 | -4.37 | -2.17 | -7.13 | -4.29 | -6.81 | -10.16 | -6.92 | -28.40 | -21.59 | -23.24 |
Peroxisomes | 0.07 | 5.23 | 1.67 | 1.35 | -7.72 | -2.37 | 2.90 | 0.81 | 4.07 | -5.13 | -2.07 | 6.14 | 1.94 | 4.00 | -10.04 |
Vacuole | -5.07 | -5.74 | -27.10 | -16.16 | -7.08 | 1.65 | -1.22 | -16.15 | -17.62 | -16.22 | -4.90 | -8.30 | -31.78 | -29.72 | -26.86 |
Vacuole Periphery | -1.88 | 3.59 | 1.53 | 2.36 | -1.93 | 4.52 | 5.98 | 0.69 | -3.27 | -4.56 | 2.38 | 7.77 | 0.31 | -1.62 | -5.38 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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