Standard name
Human Ortholog
Description Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0 0.06 0.06 0.05 0.05 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0 0.08 0.09 0.09 0.09 0.05 0.06 0.07 0.08 0.09 0.19 0.17 0.08 0.07 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.3 0.41 0.5 0.34 0.66 0.59 0.69 0.83 0.75 0.82 0.85 0.1 0 0.23 0.06 0 0.09 0 0 0 0 0 0
Nucleus 0.9 0.84 0.82 0.8 0.73 0.65 0.61 0.62 0.64 0.6 0.53 0.53 0.82 0.76 0.68 0.85 0.86 0.83 0.78 0.81 0.71 0.74 0.64 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0 0 0 0.09 0 0.07 0.07 0.1 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.11 0.07 0.13 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0
Bud 0 0 1 2 5 5 9 12 7 10 3 9 0 0 1 0 0 1 1 2 0 0 1 0
Bud Neck 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Bud Site 0 1 4 7 7 13 10 22 19 19 10 29 0 0 1 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0
Cytoplasm 9 16 9 8 24 21 30 37 14 22 16 25 7 21 22 52 33 52 1 4 1 0 0 1
Endoplasmic Reticulum 0 2 3 2 0 1 0 0 1 0 1 1 0 1 0 14 16 19 0 0 1 0 0 0
Endosome 3 0 2 1 6 0 1 0 0 1 0 0 0 1 0 14 13 9 2 7 2 1 4 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 1 1 0 0 0 0
Mitochondria 7 128 82 98 106 156 189 272 248 270 181 264 8 4 30 35 17 53 1 12 5 0 4 2
Nucleus 151 355 163 158 227 152 196 248 191 215 118 164 66 86 89 524 403 476 136 360 152 37 111 71
Nuclear Periphery 2 0 0 2 2 1 7 5 4 3 2 4 0 0 0 2 1 0 0 1 0 0 0 0
Nucleolus 8 5 4 5 8 6 5 12 5 19 4 6 2 5 8 3 2 5 15 13 15 3 16 14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0
SpindlePole 0 6 4 8 8 3 11 8 2 6 5 7 0 1 1 12 15 14 1 4 3 0 5 7
Vac/Vac Membrane 5 11 1 2 5 4 7 3 3 1 2 3 3 3 5 18 19 16 8 23 23 3 23 17
Unique Cell Count 168 422 199 197 312 235 320 397 298 361 221 311 80 113 131 619 469 572 175 444 214 51 174 133
Labelled Cell Count 185 524 273 293 398 363 465 621 494 566 342 512 86 122 157 679 522 649 175 444 214 51 174 133


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.3 4.1 3.9 4.4 3.5 3.8 3.5 3.0 3.2 3.1 2.9 4.8 5.2 4.3 6.5 6.9 7.1 5.2 5.4 6.2
Std Deviation (1e-4) 1.1 1.1 1.9 1.8 2.5 1.5 2.3 1.6 1.1 1.7 1.6 1.7 0.9 1.4 1.0 1.3 1.5 1.8 1.3 1.4 1.9
Intensity Change (Log2) – – – -0.09 0.09 -0.22 -0.12 -0.24 -0.46 -0.36 -0.39 -0.52 0.23 0.33 0.06 0.67 0.75 0.78 0.33 0.41 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.5 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6509 0.9441 0.6784 0.3284 1.2774 0.5997 1.4774 2.1256 2.0181 1.7773 1.9339 1.7854 3.461 3.2451 3.0296 2.6944 2.4378 2.9256
Actin 0.0153 0.0001 0.0206 0.0266 0 0.0013 0.0012 0 0.0028 0.017 0.005 0.0036 0.0283 0.0002 0.0043 0.0375 0.0001 0
Bud 0.0005 0.0001 0.0006 0.0003 0 0 0.0004 0 0.0006 0.0002 0.0002 0.0002 0.0002 0 0.0003 0.0013 0 0
Bud Neck 0.0098 0.0007 0.0009 0.0021 0.0003 0.0021 0.0036 0.0003 0.0007 0.0006 0.0007 0.0061 0.001 0.0002 0.0005 0.0031 0.0007 0.0028
Bud Periphery 0.0014 0.0002 0.0013 0.0003 0 0.0001 0.0018 0 0.0014 0.0003 0.0002 0.0002 0.0004 0.0001 0.0008 0.0045 0 0.0001
Bud Site 0.0046 0.0011 0.0078 0.0035 0 0.0001 0.0015 0.0001 0.0025 0.001 0.0004 0.0001 0.0014 0.0002 0.0003 0.0021 0.0001 0.0001
Cell Periphery 0.0006 0.0001 0.0008 0.0001 0 0 0.0002 0 0.0004 0.0001 0 0 0.0001 0 0.0001 0.0006 0 0
Cytoplasm 0.0063 0.006 0.0026 0.0089 0.0003 0.0008 0.0037 0.0008 0.0042 0.0007 0.0004 0.0051 0.0011 0.0009 0.0021 0.0007 0.0016 0.0007
Cytoplasmic Foci 0.0044 0.0004 0.0012 0.0336 0 0.0007 0.0022 0 0.0077 0.0026 0.0251 0.0008 0.0131 0 0.0025 0.0088 0.0003 0.0001
Eisosomes 0.0003 0 0.0003 0.0002 0 0.0001 0 0 0.0001 0.0002 0 0 0.0002 0 0 0.0004 0 0
Endoplasmic Reticulum 0.003 0.0005 0.0005 0.0007 0 0.0002 0.001 0 0.0011 0.0004 0.0002 0.0005 0.0009 0 0.0008 0.0011 0.0001 0
Endosome 0.0102 0.0009 0.004 0.0142 0 0.0018 0.0092 0 0.0165 0.0071 0.0219 0.0014 0.0153 0.0001 0.0032 0.0055 0.0005 0.0001
Golgi 0.0089 0 0.0023 0.009 0 0.0004 0.0008 0 0.0037 0.0038 0.0227 0.0007 0.0073 0 0.0014 0.0069 0 0
Lipid Particles 0.0057 0.0002 0.0023 0.022 0 0.0045 0.0018 0 0.0032 0.0092 0.033 0.0002 0.0122 0 0.0015 0.0157 0.0001 0
Mitochondria 0.0046 0.0006 0.0159 0.0014 0.0001 0.0012 0.006 0.0001 0.0188 0.0045 0.0067 0.003 0.0049 0.0003 0.0026 0.0376 0.0002 0.0002
None 0.0133 0.0246 0.0047 0.0297 0.0001 0.014 0.0132 0.0004 0.0066 0.0055 0.0002 0.0038 0.0013 0.0092 0.0154 0.0005 0.0017 0.0019
Nuclear Periphery 0.0128 0.008 0.0054 0.0058 0.0012 0.004 0.012 0.0023 0.0036 0.0029 0.0066 0.0022 0.004 0.0039 0.01 0.0039 0.0039 0.0009
Nucleolus 0.0273 0.0219 0.0241 0.0221 0.0183 0.039 0.0265 0.0157 0.0224 0.0109 0.0329 0.0352 0.0116 0.0238 0.0191 0.0176 0.0391 0.0361
Nucleus 0.8458 0.9074 0.8773 0.7332 0.9781 0.9134 0.8888 0.9682 0.8691 0.8961 0.7742 0.9153 0.8741 0.9576 0.9013 0.8349 0.9242 0.9459
Peroxisomes 0.0017 0 0.0075 0.0276 0 0.0012 0.0007 0 0.0068 0.0035 0.0639 0.0001 0.0127 0 0.0117 0.0124 0 0
Punctate Nuclear 0.0198 0.0264 0.014 0.0582 0.0013 0.0146 0.0205 0.0119 0.0196 0.0318 0.005 0.0209 0.0083 0.0032 0.0151 0.0022 0.027 0.0108
Vacuole 0.0026 0.0006 0.0044 0.0004 0.0001 0.0003 0.0034 0.0001 0.0063 0.001 0.0004 0.0004 0.0011 0.0001 0.0042 0.0015 0.0002 0.0002
Vacuole Periphery 0.0009 0.0001 0.0012 0.0002 0 0.0002 0.0018 0 0.0019 0.0005 0.0004 0.0002 0.0005 0.0001 0.0028 0.0013 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.3938 10.5676 12.4282 13.9469 14.604 14.9358 17.3882 18.2331 14.3473 18.9731
Translational Efficiency 0.4989 0.6117 0.4468 0.3439 0.3326 0.4201 0.3849 0.2787 0.295 0.2985

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
338 1297 1838 1323 1456 1813 745 222 1794 3110 2583 1545

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 649.59 773.98 1032.97 936.09 678.53 792.10 898.14 848.92 673.08 784.54 994.08 923.56
Standard Deviation 108.54 114.49 133.28 121.88 104.96 104.94 120.25 126.42 106.24 109.39 143.32 126.30
Intensity Change Log 2 — 0.252767 0.669197 0.527118 — 0.223270 0.404528 0.323216 — 0.237772 0.540045 0.426547

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000384 0.000628 0.000774 0.001570 0.000174 0.000366 0.000900 0.000809 0.000213 0.000476 0.000811 0.001461
Bud Neck 0.011876 0.006659 0.022139 0.038439 0.003629 0.004688 0.010097 0.005683 0.005183 0.005510 0.018666 0.033732
Bud Site 0.006083 0.001567 0.002562 0.014354 0.000929 0.001884 0.001935 0.002186 0.001900 0.001752 0.002381 0.012606
Cell Periphery 0.000178 0.000167 0.000062 0.000271 0.000101 0.000066 0.000130 0.000221 0.000116 0.000108 0.000082 0.000264
Cytoplasm 0.030251 0.017093 0.003729 0.011109 0.023415 0.013509 0.007910 0.051607 0.024703 0.015004 0.004935 0.016929
Cytoplasmic Foci 0.008544 0.003222 0.000049 0.006978 0.002496 0.003049 0.000446 0.003511 0.003635 0.003121 0.000164 0.006480
Eisosomes 0.000040 0.000032 0.000027 0.000040 0.000016 0.000025 0.000038 0.000060 0.000021 0.000028 0.000030 0.000042
Endoplasmic Reticulum 0.004912 0.003990 0.004735 0.008708 0.001321 0.002851 0.005482 0.004826 0.001998 0.003326 0.004950 0.008150
Endosome 0.004046 0.001052 0.000250 0.009474 0.000257 0.001951 0.000656 0.000968 0.000971 0.001576 0.000367 0.008252
Golgi 0.001438 0.000577 0.000093 0.005685 0.000207 0.000554 0.000157 0.001403 0.000439 0.000563 0.000112 0.005070
Lipid Particles 0.003376 0.001184 0.000142 0.000606 0.000775 0.002501 0.000370 0.001908 0.001265 0.001952 0.000208 0.000793
Mitochondria 0.003274 0.001964 0.001311 0.006938 0.001309 0.006024 0.002674 0.004272 0.001679 0.004331 0.001704 0.006555
Mitotic Spindle 0.000694 0.002631 0.001000 0.070150 0.001102 0.008804 0.002192 0.014738 0.001025 0.006230 0.001344 0.062188
None 0.016957 0.010749 0.002103 0.006188 0.018583 0.006014 0.011987 0.027983 0.018277 0.007989 0.004954 0.009319
Nuclear Periphery 0.002080 0.001419 0.000653 0.004369 0.001057 0.001593 0.000668 0.001051 0.001250 0.001520 0.000657 0.003892
Nuclear Periphery Foci 0.001066 0.000958 0.000160 0.002210 0.000306 0.001078 0.000661 0.003338 0.000449 0.001028 0.000304 0.002372
Nucleolus 0.034924 0.022627 0.012749 0.014429 0.018329 0.022307 0.015427 0.012384 0.021456 0.022440 0.013521 0.014135
Nucleus 0.860930* 0.919741* 0.944740* 0.783721* 0.925156* 0.918001* 0.934607* 0.855573* 0.913055* 0.918727* 0.941818* 0.794046*
Peroxisomes 0.001194 0.001426 0.000193 0.001510 0.000262 0.001418 0.000441 0.001765 0.000438 0.001421 0.000265 0.001546
Vacuole 0.006645 0.001784 0.002369 0.010909 0.000482 0.002543 0.002457 0.005331 0.001643 0.002226 0.002394 0.010108
Vacuole Periphery 0.001106 0.000530 0.000159 0.002341 0.000095 0.000777 0.000766 0.000385 0.000285 0.000674 0.000334 0.002060

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.46 -9.19 -13.27 -10.11 -8.24 -1.80 -11.21 -5.39 -4.76 -2.54 -3.41 -15.22 -16.02 -12.66 -8.23
Bud Neck 1.53 -4.68 -9.27 -17.79 -8.15 -1.76 -7.95 -7.39 -6.70 0.08 -0.47 -16.55 -18.82 -19.40 -9.09
Bud Site 1.77 1.17 -1.74 -7.50 -6.12 -1.34 -2.37 -4.91 -3.96 -3.89 0.08 -1.48 -6.90 -7.74 -6.79
Cell Periphery 0.25 2.76 0.17 -0.10 -3.83 1.81 -0.83 -2.85 -4.08 -2.43 0.31 2.03 -2.14 -2.53 -3.92
Cytoplasm 2.31 5.34 3.53 2.90 -7.70 3.93 6.72 -1.96 -3.23 -4.14 4.27 10.65 3.87 -0.07 -7.56
Cytoplasmic Foci 2.15 4.21 3.17 2.06 -3.37 -1.13 4.40 -0.36 0.35 -2.68 0.16 6.38 2.18 2.29 -3.90
Eisosomes 1.86 2.10 -3.80 -6.81 -9.18 -4.97 -10.64 -7.80 -6.53 -3.88 -4.37 -9.95 -16.43 -12.03 -9.15
Endoplasmic Reticulum 0.71 -0.76 -6.22 -13.69 -11.74 -5.75 -14.95 -7.68 -6.26 -2.93 -5.37 -15.56 -19.90 -16.96 -11.58
Endosome 1.70 2.22 0.27 -3.47 -5.02 -4.59 -2.68 -4.55 1.35 -2.51 -1.79 1.65 -3.60 -2.60 -5.10
Golgi 0.76 1.21 -0.34 -1.98 -2.92 -1.91 0.23 -1.18 -0.59 -1.25 -0.59 1.26 -2.30 -2.16 -3.08
Lipid Particles 2.20 3.60 3.14 3.47 -5.91 -3.34 1.67 -1.89 1.36 -2.99 -2.11 4.07 1.96 4.24 -5.83
Mitochondria 1.40 1.93 -2.56 -6.69 -8.78 -4.22 -1.21 -2.05 1.36 -1.14 -3.62 -0.43 -5.96 -1.33 -7.45
Mitotic Spindle -2.71 -3.33 -12.26 -11.72 -11.53 -4.25 -1.76 -3.91 -2.83 -3.69 -4.70 -3.21 -12.72 -11.40 -12.11
None 1.67 3.64 2.67 3.50 -7.27 5.73 3.64 -0.27 -3.18 -2.41 5.37 7.43 5.02 -0.38 -4.48
Nuclear Periphery 1.16 2.11 -5.36 -10.35 -12.68 -2.91 0.50 -2.92 -1.69 -3.11 -1.58 2.08 -11.22 -10.65 -12.98
Nuclear Periphery Foci -0.14 2.45 -2.51 -2.55 -4.34 -3.49 -2.71 -2.14 -1.64 -1.81 -3.65 0.06 -4.49 -3.10 -4.51
Nucleolus 3.18 4.64 3.33 0.29 -2.21 -1.86 0.20 -1.16 -0.44 -1.21 -0.48 2.23 -0.43 -0.04 -2.52
Nucleus -4.39 -5.58 5.38 15.30 18.40 1.31 -0.47 6.20 5.70 6.30 -0.77 -3.36 15.50 16.97 19.38
Peroxisomes -0.88 5.07 2.33 1.99 -3.29 -4.30 -2.04 -1.98 -0.02 -1.73 -4.24 2.87 -2.32 2.60 -3.46
Vacuole 2.63 1.57 -3.53 -9.21 -7.71 -4.97 -4.27 -4.72 -3.71 -2.73 -1.77 -4.37 -10.55 -9.84 -8.06
Vacuole Periphery 1.69 3.37 0.67 -1.36 -4.11 -4.46 -1.52 -2.68 2.11 0.57 -3.11 -0.09 -3.36 -0.65 -2.96
Standard name
Human Ortholog
Description Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Hir3

Hir3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hir3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available