Standard name
Human Ortholog
Description Htz1p-binding component of the SWR1 complex; exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; may function as a lock that prevents removal of H2AZ from nucleosomes; required for vacuolar protein sorting
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0 – 0.05 0.09 0 0.13 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.12 0.1 0.22 – 0.07 0.12 0.1 0.1 0.05 0.09 0.14 0.07 0.12 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.24 0.07 0.24 0.09 0.39 0.41 – 0.62 0.65 0.73 0.64 0.18 0.06 0.05 0 0 0.07 0 0 0 0 0 0
Nucleus 0.88 0.84 0.93 0.88 0.81 0.65 0.59 – 0.7 0.63 0.6 0.59 0.87 0.85 0.8 0.78 0.78 0.8 0.69 0.73 0.68 0.63 0.52 0.39
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.13 0.05 0 0 0 0 0 – 0.05 0 0 0 0.07 0.06 0.07 0 0.08 0.1 0.18 0.1 0.14 0.15 0.23 0.2
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0 0.05 0.07 0.06 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.06 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.07 0.05 0.07 0.06 0.1 0.09 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 3
Bud 0 0 0 0 0 1 5 – 2 6 3 1 0 0 0 1 1 0 0 1 0 1 2 8
Bud Neck 0 0 1 0 1 0 3 – 1 4 0 1 0 0 0 0 0 0 0 0 0 4 3 6
Bud Site 1 0 1 0 1 6 7 – 8 18 4 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 6 4 2 15 13 40 – 11 23 14 7 13 23 38 7 6 3 0 1 0 0 1 10
Endoplasmic Reticulum 1 2 0 0 0 0 0 – 0 0 0 0 0 1 0 4 0 4 0 0 0 0 0 0
Endosome 0 1 1 0 0 2 0 – 0 0 0 0 0 1 2 5 1 0 0 6 4 0 4 7
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 1 1 0 1 0 0 1 0 1 0 0
Mitochondria 16 54 8 8 12 49 76 – 104 128 102 45 43 14 15 4 2 3 0 5 2 0 4 6
Nucleus 102 187 115 30 104 82 108 – 117 125 83 41 213 215 221 76 40 33 84 185 100 107 108 95
Nuclear Periphery 0 1 0 0 0 0 0 – 0 0 1 0 1 0 1 1 0 1 0 0 0 0 0 1
Nucleolus 15 11 1 0 0 3 4 – 8 5 5 2 18 16 19 3 4 4 22 24 21 25 46 47
Peroxisomes 0 0 0 0 0 1 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 2 12 0 6 9 11 – 4 8 6 2 6 6 4 3 0 3 4 5 5 7 13 20
Vac/Vac Membrane 3 4 0 0 3 1 1 – 3 2 1 1 0 3 3 7 1 3 5 18 9 17 18 28
Unique Cell Count 116 223 123 34 128 127 184 167 197 139 70 244 252 275 98 51 41 122 254 148 170 208 245
Labelled Cell Count 142 270 143 40 142 167 255 258 319 219 109 294 281 304 111 56 54 122 254 148 170 208 245


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.7 5.0 4.3 5.4 4.3 4.2 – 4.0 4.0 3.6 3.7 4.7 5.0 4.8 7.0 6.9 6.7 4.6 4.7 5.0
Std Deviation (1e-4) 0.9 0.9 0.6 0.6 1.6 1.5 1.6 – 1.7 1.9 1.4 0.9 1.0 1.3 1.2 1.6 1.6 1.6 1.3 1.6 2.5
Intensity Change (Log2) – – – -0.22 0.12 -0.2 -0.24 – -0.32 -0.32 -0.45 -0.43 -0.06 0.01 -0.05 0.5 0.48 0.44 -0.11 -0.08 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9853 3.0909 2.917 2.1672 2.8962 2.7584 0.8031 0.8304 0.8018 0.2743 0.248 0.4707 0.9428 0.793 0.5053 0.7272 0.4187 0.4841
Actin 0.0145 0 0.0038 0.0007 0.0059 0.0088 0.0171 0 0.0107 0.0005 0.0007 0.0012 0.0305 0 0.007 0.0003 0.0046 0.0052
Bud 0.0002 0 0.0004 0.0001 0.0009 0.0001 0.0006 0 0.0005 0.0003 0.0006 0.0001 0.0005 0.0001 0.0002 0 0.0003 0
Bud Neck 0.0018 0.0002 0.0008 0.0001 0.0078 0.0023 0.0012 0.0001 0.001 0.0005 0.0006 0.0012 0.0038 0.0002 0.0002 0.0002 0.0008 0.0013
Bud Periphery 0.0004 0 0.0006 0.0005 0.0021 0.0002 0.0012 0 0.0014 0.0015 0.0035 0.0002 0.0013 0.0001 0.0005 0.0001 0.0005 0.0001
Bud Site 0.0009 0.0001 0.0031 0.0003 0.0044 0.0002 0.002 0.0001 0.0032 0.0003 0.0004 0.0002 0.0024 0.0002 0.0006 0.0001 0.0007 0.0001
Cell Periphery 0.0002 0 0.0001 0.0001 0.0004 0 0.0002 0 0.0003 0.0002 0.0001 0.0001 0.0003 0 0 0 0.0001 0
Cytoplasm 0.0052 0.0006 0.0018 0 0.0062 0.0007 0.0033 0.0015 0.0019 0.0002 0.0005 0.0003 0.0037 0.0004 0.0007 0.0001 0.0004 0.0045
Cytoplasmic Foci 0.003 0.0001 0.0016 0.0021 0.0064 0.0014 0.0123 0 0.0049 0.0001 0.002 0.0014 0.0042 0 0.002 0.0017 0.0023 0.0016
Eisosomes 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0002 0 0.0001 0 0 0 0.0006 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0039 0.0001 0.0012 0 0.0023 0.0004 0.0043 0.0001 0.0019 0.0004 0.0008 0.0002 0.0025 0.0001 0.0002 0 0.0004 0.0006
Endosome 0.0143 0.0003 0.0075 0.0006 0.0202 0.0041 0.0296 0.0001 0.0174 0.0017 0.0096 0.0031 0.0168 0.0003 0.0021 0.0015 0.0297 0.0013
Golgi 0.002 0 0.0029 0.0007 0.0102 0.0017 0.0098 0 0.0101 0.0003 0.0013 0.0022 0.0046 0 0.0015 0.0012 0.0103 0.001
Lipid Particles 0.0075 0.0002 0.0051 0.0034 0.0198 0.0064 0.01 0 0.0114 0.0006 0.0048 0.0014 0.0066 0 0.0033 0.0158 0.0055 0.0063
Mitochondria 0.0122 0.0002 0.0057 0.0355 0.0423 0.0036 0.0125 0.0002 0.014 0.0045 0.0127 0.0036 0.0034 0.0003 0.0064 0.0006 0.034 0.0029
None 0.0054 0.0022 0.005 0 0.0027 0.0012 0.001 0.0014 0.0004 0.0002 0.0004 0.0001 0.0061 0.0001 0.0018 0.0001 0.0001 0.0001
Nuclear Periphery 0.0242 0.0039 0.0054 0.0003 0.0371 0.0027 0.0152 0.0021 0.0052 0.0138 0.0233 0.0029 0.0112 0.0036 0.0012 0.0029 0.0017 0.0019
Nucleolus 0.0339 0.0319 0.035 0.0137 0.0503 0.0664 0.0217 0.025 0.0181 0.0194 0.0662 0.0492 0.035 0.0211 0.0287 0.0176 0.0343 0.0528
Nucleus 0.8246 0.9536 0.9038 0.9054 0.7327 0.8776 0.8398 0.9664 0.8855 0.9466 0.8528 0.9268 0.8455 0.9695 0.9342 0.9438 0.8668 0.9136
Peroxisomes 0.0068 0 0.0012 0.0346 0.0134 0.0027 0.0047 0 0.0033 0.0001 0.0004 0.0004 0.0055 0 0.004 0.0026 0.0017 0.0012
Punctate Nuclear 0.0318 0.0061 0.0129 0.0007 0.0275 0.0187 0.0056 0.0027 0.0031 0.0021 0.0121 0.0045 0.0107 0.0035 0.0047 0.011 0.002 0.0047
Vacuole 0.0044 0.0004 0.0013 0.0005 0.004 0.0005 0.005 0.0001 0.0035 0.0032 0.0034 0.0006 0.0035 0.0002 0.0003 0.0001 0.0023 0.0002
Vacuole Periphery 0.0024 0.0001 0.0008 0.0004 0.0033 0.0003 0.0028 0.0001 0.002 0.0037 0.0037 0.0003 0.0012 0.0003 0.0003 0.0001 0.0013 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.5516 7.8481 7.0496 11.4574 10.7835 9.2361 9.0837 12.2617 10.8882 11.1528
Translational Efficiency 0.8579 1.2661 1.5861 0.9957 0.9849 1.2999 1.2208 0.9654 1.0966 1.2119

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
368 1753 980 1348 1501 1784 1958 117 1869 3537 2938 1465

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 671.26 743.71 791.39 872.51 723.88 757.89 853.53 923.01 713.52 750.86 832.80 876.54
Standard Deviation 69.89 102.05 103.18 110.47 85.19 108.55 99.04 96.99 85.02 105.62 104.62 110.30
Intensity Change Log 2 — 0.147868 0.237517 0.378300 — 0.066238 0.237691 0.350596 — 0.106091 0.237608 0.363992

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000159 0.000591 0.000606 0.001538 0.000179 0.000513 0.000530 0.002913 0.000175 0.000551 0.000555 0.001648
Bud Neck 0.000996 0.008710 0.008013 0.032174 0.002295 0.008477 0.017096 0.014933 0.002039 0.008592 0.014066 0.030797
Bud Site 0.000286 0.001590 0.002365 0.014160 0.000320 0.003019 0.001708 0.014080 0.000313 0.002311 0.001927 0.014153
Cell Periphery 0.000098 0.000089 0.000189 0.000155 0.000076 0.000146 0.000070 0.000152 0.000080 0.000118 0.000110 0.000155
Cytoplasm 0.026379 0.019413 0.016094 0.014100 0.017314 0.018302 0.004631 0.031482 0.019099 0.018852 0.008454 0.015488
Cytoplasmic Foci 0.008880 0.002770 0.002975 0.000597 0.004626 0.006874 0.000261 0.001520 0.005463 0.004840 0.001167 0.000670
Eisosomes 0.000038 0.000030 0.000042 0.000035 0.000031 0.000042 0.000028 0.000081 0.000032 0.000036 0.000032 0.000039
Endoplasmic Reticulum 0.000856 0.002509 0.002410 0.008225 0.001353 0.002897 0.002889 0.013430 0.001255 0.002704 0.002730 0.008641
Endosome 0.000876 0.000775 0.000776 0.002843 0.000492 0.001570 0.000311 0.001888 0.000568 0.001176 0.000466 0.002766
Golgi 0.000529 0.000528 0.000225 0.001805 0.000501 0.001131 0.000115 0.000238 0.000507 0.000832 0.000151 0.001680
Lipid Particles 0.004798 0.001496 0.001372 0.000387 0.001780 0.002363 0.000161 0.001895 0.002375 0.001933 0.000565 0.000508
Mitochondria 0.010394 0.004087 0.000992 0.004135 0.002455 0.010642 0.001018 0.003209 0.004018 0.007393 0.001009 0.004061
Mitotic Spindle 0.007737 0.003784 0.002183 0.032995 0.001079 0.010316 0.002212 0.028545 0.002390 0.007079 0.002202 0.032639
None 0.018362 0.012390 0.017173 0.006196 0.013504 0.013080 0.005507 0.012605 0.014460 0.012738 0.009399 0.006708
Nuclear Periphery 0.000566 0.001355 0.000334 0.002459 0.001303 0.001603 0.000527 0.004179 0.001158 0.001480 0.000462 0.002596
Nuclear Periphery Foci 0.000406 0.001720 0.000425 0.000872 0.001033 0.001476 0.000237 0.001822 0.000910 0.001597 0.000300 0.000948
Nucleolus 0.036646 0.053497 0.050415 0.013989 0.052375 0.063165 0.040567 0.015656 0.049278 0.058374 0.043852 0.014122
Nucleus 0.877345* 0.881782* 0.891042* 0.848843* 0.897575* 0.847917* 0.920934* 0.814443* 0.893592* 0.864701* 0.910964* 0.846096*
Peroxisomes 0.003823 0.001697 0.001255 0.001011 0.000955 0.003958 0.000249 0.000782 0.001520 0.002837 0.000585 0.000993
Vacuole 0.000641 0.000800 0.000977 0.011309 0.000559 0.001709 0.000747 0.035547 0.000575 0.001259 0.000824 0.013244
Vacuole Periphery 0.000183 0.000388 0.000138 0.002171 0.000195 0.000801 0.000200 0.000601 0.000193 0.000597 0.000180 0.002046

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.58 -11.21 -10.20 -6.46 -6.54 -5.62 -13.30 -5.69 -5.15 -4.95 -6.75 -17.21 -11.46 -8.51 -8.15
Bud Neck -12.67 -10.89 -19.80 -14.83 -13.70 -7.48 -15.93 -8.12 -5.79 -1.71 -12.67 -18.37 -20.04 -15.81 -10.96
Bud Site -4.87 -2.93 -7.93 -6.66 -5.29 -3.60 -6.80 -5.05 -4.11 -4.35 -5.09 -6.47 -8.74 -6.77 -6.74
Cell Periphery 0.43 -1.82 -2.36 -2.50 0.88 -2.40 0.74 -3.59 -1.15 -4.24 -1.91 -1.11 -3.97 -1.30 -2.27
Cytoplasm 1.42 2.68 2.92 2.73 0.17 -0.44 7.29 -1.01 -0.79 -4.29 0.07 6.69 2.58 2.93 -5.07
Cytoplasmic Foci 2.84 3.01 4.03 5.72 4.51 -1.81 5.76 4.46 6.63 -1.77 0.86 6.06 6.85 9.48 3.07
Eisosomes 2.19 -1.40 -2.87 -7.43 -1.94 -3.67 0.87 -4.58 -4.04 -4.69 -1.39 -0.69 -7.36 -6.74 -7.69
Endoplasmic Reticulum -8.52 -8.93 -21.90 -18.50 -16.96 -6.33 -12.27 -7.97 -7.11 -6.83 -9.54 -14.55 -23.14 -19.44 -18.36
Endosome 0.11 0.13 -1.68 -2.41 -2.06 -3.78 2.87 -4.71 -0.69 -5.82 -3.18 1.00 -3.11 -1.54 -3.50
Golgi -0.12 1.44 -0.58 -0.54 -1.48 -2.45 2.07 -0.01 3.03 -5.26 -1.87 2.28 -0.67 0.36 -1.76
Lipid Particles 2.43 2.53 3.27 3.30 1.17 -1.79 8.27 -0.23 0.40 -2.31 1.35 5.32 5.78 6.64 -0.51
Mitochondria 1.42 2.17 1.49 0.39 -10.19 -5.67 2.77 -1.46 4.13 -3.22 -2.90 3.17 0.11 4.48 -11.39
Mitotic Spindle 0.66 1.35 -4.97 -7.42 -8.27 -5.19 -2.83 -4.65 -4.16 -4.52 -4.11 -0.95 -9.43 -7.86 -9.27
None 1.85 0.92 4.11 6.84 4.40 0.48 6.26 0.99 0.73 -2.72 1.41 4.46 6.66 8.35 2.27
Nuclear Periphery -2.78 1.25 -11.19 -6.78 -12.54 -0.99 2.43 -3.19 -2.88 -4.05 -1.31 2.73 -8.49 -7.95 -12.57
Nuclear Periphery Foci -3.70 -1.00 -3.89 1.71 -3.12 -1.10 3.55 -1.27 -0.95 -2.07 -2.46 3.17 -1.18 1.41 -4.79
Nucleolus -4.22 -3.85 5.41 14.14 10.70 -3.20 3.51 4.96 6.81 3.15 -3.58 1.26 13.04 19.09 12.98
Nucleus -0.11 -0.83 3.05 5.25 5.96 7.13 -3.86 6.62 4.73 7.45 5.48 -2.97 8.56 4.67 11.80
Peroxisomes 1.28 1.62 1.96 2.71 1.02 -4.49 5.32 -0.15 3.66 -1.81 -2.73 2.86 2.16 5.03 -0.40
Vacuole -0.96 -2.87 -9.65 -9.56 -9.16 -3.08 -2.59 -4.47 -4.39 -4.44 -3.12 -3.75 -10.43 -10.06 -10.14
Vacuole Periphery -3.43 0.78 -2.61 -1.91 -2.71 -5.00 0.23 -3.85 2.81 -3.77 -5.93 0.61 -2.63 -1.19 -2.69
Standard name
Human Ortholog
Description Htz1p-binding component of the SWR1 complex; exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; may function as a lock that prevents removal of H2AZ from nucleosomes; required for vacuolar protein sorting
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Vps72

Vps72


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps72-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available