Standard name | |
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Human Ortholog | |
Description | High-affinity glutamine permease; also transports Leu, Ser, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids; GNP1 has a paralog, AGP1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 12 | 37 | 9 | 41 | 108 | 175 | 146 | 88 | 94 | 56 | 82 | 12 | 24 | 9 | 4 | 7 | 0 | 1 | 6 | 4 | 1 | 4 | 4 |
Bud | 2 | 5 | 2 | 4 | 5 | 11 | 29 | 51 | 41 | 44 | 52 | 44 | 4 | 3 | 3 | 7 | 2 | 2 | 0 | 1 | 1 | 1 | 4 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 177 | 197 | 248 | 176 | 261 | 279 | 336 | 264 | 164 | 137 | 125 | 152 | 169 | 261 | 237 | 148 | 113 | 89 | 151 | 174 | 267 | 184 | 375 | 400 |
Cytoplasm | 17 | 61 | 15 | 12 | 32 | 38 | 81 | 124 | 128 | 161 | 169 | 235 | 19 | 35 | 28 | 31 | 67 | 25 | 5 | 58 | 1 | 23 | 15 | 5 |
Endoplasmic Reticulum | 25 | 25 | 38 | 55 | 36 | 13 | 39 | 127 | 84 | 96 | 91 | 67 | 41 | 69 | 35 | 5 | 5 | 7 | 58 | 69 | 83 | 80 | 77 | 26 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 6 | 3 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 0 | 3 | 0 | 0 | 1 | 2 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 13 | 6 | 13 | 18 | 14 | 0 | 0 | 0 | 10 | 14 | 15 | 2 | 2 | 15 | 20 | 39 | 26 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 12 | 1 | 8 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 5 | 3 | 3 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 6 | 36 | 53 | 85 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 2 | 4 | 2 |
Unique Cell Count | 200 | 240 | 287 | 229 | 314 | 319 | 456 | 525 | 381 | 449 | 430 | 528 | 198 | 306 | 268 | 177 | 182 | 121 | 223 | 317 | 380 | 319 | 528 | 483 |
Labelled Cell Count | 229 | 300 | 340 | 256 | 377 | 450 | 668 | 750 | 536 | 608 | 581 | 707 | 245 | 393 | 313 | 207 | 211 | 144 | 223 | 317 | 380 | 319 | 528 | 483 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 67.8 | 56.7 | 63.7 | 65.2 | 48.9 | 34.3 | 27.0 | 21.8 | 19.0 | 16.4 | 14.5 | 14.0 | 61.2 | 62.8 | 66.9 | 37.3 | 45.7 | 55.0 | 67.5 | 69.5 | 66.3 |
Std Deviation (1e-4) | 15.1 | 12.9 | 14.7 | 12.6 | 11.9 | 9.3 | 8.6 | 7.5 | 7.8 | 7.2 | 6.6 | 6.2 | 13.4 | 16.5 | 14.0 | 10.0 | 14.3 | 19.0 | 13.4 | 16.5 | 16.7 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.38 | -0.89 | -1.24 | -1.54 | -1.74 | -1.96 | -2.14 | -2.18 | -0.06 | -0.02 | 0.07 | -0.77 | -0.48 | -0.21 | 0.08 | 0.12 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.5 | 0.1 | 6.0 | 7.5 | 4.9 | 3.3 | 2.8 | 0.1 | 1.0 | -2.5 | -2.0 | -4.1 | -3.9 | -3.3 | -4.1 |
Bud | 0 | 0 | 2.3 | 3.8 | 5.0 | 5.2 | 5.0 | 5.7 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.8 | -1.1 | 0.4 | -4.1 | -10.2 | -11.4 | -14.8 | -15.1 | -15.7 | -0.3 | -0.4 | 0.7 | -0.8 | -6.1 | -3.1 |
Cytoplasm | 0 | 2.3 | 2.9 | 5.0 | 6.7 | 8.8 | 9.5 | 10.2 | 11.6 | 1.9 | 2.7 | 2.3 | 4.3 | 8.8 | 4.8 |
Endoplasmic Reticulum | 3.2 | -0.7 | -4.1 | -2.0 | 3.7 | 2.9 | 2.8 | 2.7 | -0.2 | 2.2 | 2.9 | -0.1 | -3.8 | -3.8 | -2.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 3.5 | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 | 6.2 | 7.2 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 118.6286 | 138.79 | 129.6599 | 131.9131 | 74.5854 | 134.0688 | 115.6522 | 139.9555 | 136.6626 | 127.8223 | 101.9009 | 122.2285 | 131.6169 | 152.578 | 145.7363 | 140.4688 | 140.0306 | 146.7665 |
Actin | 0.0028 | 0 | 0 | 0.0003 | 0.0205 | 0.0001 | 0.0051 | 0.0002 | 0.0001 | 0.0002 | 0.015 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0012 | 0.0002 | 0.0004 | 0 | 0.0005 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0006 | 0.0003 | 0.0006 | 0.0033 | 0.007 | 0.001 | 0.0009 | 0.0005 | 0.0014 | 0.0009 | 0.0029 | 0.0029 | 0.0002 | 0.0003 | 0.0005 | 0.0008 | 0.0009 | 0.0008 |
Bud Periphery | 0.0019 | 0.0005 | 0.0003 | 0.0008 | 0.0041 | 0.0008 | 0.0034 | 0.0002 | 0.002 | 0.001 | 0.0039 | 0.0036 | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0.0007 | 0.0007 |
Bud Site | 0.001 | 0.0054 | 0.0002 | 0.0003 | 0.0033 | 0.0002 | 0.0018 | 0.004 | 0.0004 | 0.0005 | 0.0075 | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0 | 0.0001 | 0 |
Cell Periphery | 0.5372 | 0.6472 | 0.5883 | 0.6081 | 0.3678 | 0.5506 | 0.5184 | 0.6376 | 0.6097 | 0.5092 | 0.5339 | 0.5889 | 0.5121 | 0.6039 | 0.5615 | 0.5074 | 0.5187 | 0.5277 |
Cytoplasm | 0.0038 | 0.0079 | 0.01 | 0.0014 | 0.0197 | 0.0044 | 0.0045 | 0.0064 | 0.0014 | 0.0006 | 0.0048 | 0.0025 | 0.0016 | 0.0016 | 0.001 | 0.0005 | 0.001 | 0.0007 |
Cytoplasmic Foci | 0.0029 | 0 | 0.0039 | 0 | 0.0225 | 0.0008 | 0.0092 | 0.0001 | 0.0001 | 0.0002 | 0.0214 | 0.0028 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.0092 | 0.0055 | 0.0031 | 0.0034 | 0.0118 | 0.0126 | 0.0192 | 0.0076 | 0.006 | 0.0094 | 0.0038 | 0.0102 | 0.0101 | 0.0199 | 0.0084 | 0.0054 | 0.032 | 0.0064 |
Endoplasmic Reticulum | 0.146 | 0.0987 | 0.1347 | 0.1158 | 0.1341 | 0.2136 | 0.1535 | 0.1106 | 0.1098 | 0.2061 | 0.1492 | 0.175 | 0.2129 | 0.1025 | 0.1524 | 0.2254 | 0.2436 | 0.2718 |
Endosome | 0.0006 | 0 | 0 | 0 | 0.0232 | 0.0001 | 0.0019 | 0 | 0 | 0.0001 | 0.0054 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.0008 | 0 | 0 | 0.0001 | 0.0079 | 0.0001 | 0.001 | 0.0003 | 0 | 0.0001 | 0.0042 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0034 | 0.001 | 0.0018 | 0.0014 | 0.0608 | 0.0025 | 0.0077 | 0.0035 | 0.0006 | 0.0019 | 0.0235 | 0.0053 | 0.0023 | 0.0005 | 0.0006 | 0.0005 | 0.0006 | 0.0011 |
Mitochondria | 0.0026 | 0 | 0.0002 | 0.0001 | 0.0307 | 0.0053 | 0.0059 | 0 | 0.0012 | 0.0227 | 0.017 | 0.0103 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
None | 0.0061 | 0 | 0.0009 | 0 | 0.0142 | 0.0026 | 0.0072 | 0 | 0.0029 | 0.001 | 0.0047 | 0.0005 | 0.0009 | 0 | 0 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.2189 | 0.1988 | 0.2129 | 0.2165 | 0.1181 | 0.1574 | 0.2059 | 0.2062 | 0.2325 | 0.2108 | 0.1211 | 0.1512 | 0.2285 | 0.2477 | 0.2476 | 0.2293 | 0.1835 | 0.1629 |
Nucleolus | 0.0098 | 0.0001 | 0.0015 | 0.0003 | 0.0543 | 0.0045 | 0.0063 | 0.0001 | 0.0004 | 0.0044 | 0.0165 | 0.0087 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0024 | 0.0005 | 0.0012 | 0.001 | 0.0096 | 0.001 | 0.0021 | 0.0006 | 0.0008 | 0.001 | 0.0023 | 0.0015 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0004 |
Peroxisomes | 0.0008 | 0 | 0.0001 | 0 | 0.0255 | 0.0003 | 0.0059 | 0 | 0.0001 | 0.0004 | 0.0196 | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0009 | 0 | 0.0006 | 0 | 0.0184 | 0 | 0.0019 | 0 | 0 | 0 | 0.0023 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0457 | 0.0337 | 0.039 | 0.0464 | 0.0407 | 0.0407 | 0.0352 | 0.0219 | 0.0293 | 0.0274 | 0.0385 | 0.0334 | 0.0289 | 0.0223 | 0.0265 | 0.0291 | 0.0181 | 0.0267 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.0004 | 0.0007 | 0.0047 | 0.0012 | 0.0023 | 0.0002 | 0.0006 | 0.0019 | 0.0018 | 0.0012 | 0.0006 | 0.0001 | 0.0004 | 0.0006 | 0.0003 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 137.3716 | 107.9468 | 123.0913 | 158.0525 | 140.0449 | 139.51 | 159.712 | 154.0892 | 178.1527 | 172.6892 |
Translational Efficiency | 1.5522 | 1.5467 | 1.4055 | 1.2564 | 1.2085 | 1.6184 | 1.1951 | 1.0613 | 1.0952 | 1.1179 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
140 | 756 | 92 | 1062 | 1943 | 152 | 1528 | 1302 | 2083 | 908 | 1620 | 2364 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2525.68 | 4587.16 | 5785.90 | 4265.60 | 3923.28 | 4961.13 | 5395.67 | 4662.57 | 3829.35 | 4649.76 | 5417.83 | 4484.24 |
Standard Deviation | 827.47 | 1130.67 | 688.43 | 980.09 | 824.27 | 1084.03 | 826.23 | 1040.18 | 895.68 | 1131.64 | 823.99 | 1032.68 |
Intensity Change Log 2 | — | 0.860929 | 1.195870 | 0.756077 | — | 0.338608 | 0.459742 | 0.249065 | — | 0.566176 | 0.793984 | 0.469298 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003259 | 0.000996 | 0.000253 | 0.001264 | 0.000133 | 0.001413 | 0.000798 | 0.002988 | 0.000343 | 0.001065 | 0.000767 | 0.002213 |
Bud Neck | 0.008557 | 0.002273 | 0.002295 | 0.000795 | 0.000469 | 0.003421 | 0.000696 | 0.000630 | 0.001012 | 0.002465 | 0.000787 | 0.000704 |
Bud Site | 0.017954 | 0.020260 | 0.002001 | 0.004321 | 0.002056 | 0.015110 | 0.003437 | 0.008602 | 0.003124 | 0.019398 | 0.003356 | 0.006679 |
Cell Periphery | 0.443126* | 0.682285* | 0.666439* | 0.725992* | 0.708496* | 0.734336* | 0.595780* | 0.859464* | 0.690661* | 0.690998* | 0.599793* | 0.799503* |
Cytoplasm | 0.011001 | 0.050172 | 0.000655 | 0.007350 | 0.017814 | 0.001501 | 0.023119 | 0.002360 | 0.017356 | 0.042025 | 0.021844 | 0.004601 |
Cytoplasmic Foci | 0.013439 | 0.000561 | 0.000094 | 0.000217 | 0.000511 | 0.000434 | 0.000392 | 0.000555 | 0.001380 | 0.000539 | 0.000376 | 0.000403 |
Eisosomes | 0.000859 | 0.002335 | 0.004980 | 0.002464 | 0.000539 | 0.005840 | 0.001252 | 0.000978 | 0.000561 | 0.002921 | 0.001464 | 0.001646 |
Endoplasmic Reticulum | 0.127950* | 0.011834 | 0.016788 | 0.015977 | 0.025640 | 0.026543 | 0.043810 | 0.006538 | 0.032517 | 0.014296 | 0.042276 | 0.010778 |
Endosome | 0.014553 | 0.000846 | 0.000713 | 0.000587 | 0.000838 | 0.001186 | 0.000739 | 0.000549 | 0.001760 | 0.000903 | 0.000738 | 0.000566 |
Golgi | 0.014906 | 0.005844 | 0.010414 | 0.003555 | 0.003379 | 0.010239 | 0.005361 | 0.004742 | 0.004154 | 0.006579 | 0.005648 | 0.004209 |
Lipid Particles | 0.097294 | 0.026033 | 0.021836 | 0.004996 | 0.004718 | 0.012195 | 0.029465 | 0.004101 | 0.010940 | 0.023716 | 0.029032 | 0.004503 |
Mitochondria | 0.000940 | 0.000767 | 0.000103 | 0.001088 | 0.000428 | 0.000764 | 0.000464 | 0.000475 | 0.000462 | 0.000767 | 0.000444 | 0.000750 |
Mitotic Spindle | 0.007677 | 0.001440 | 0.001941 | 0.001528 | 0.001702 | 0.002341 | 0.002060 | 0.002005 | 0.002103 | 0.001591 | 0.002053 | 0.001791 |
None | 0.000041 | 0.000332 | 0.000045 | 0.000173 | 0.000081 | 0.000117 | 0.000171 | 0.000048 | 0.000078 | 0.000296 | 0.000164 | 0.000104 |
Nuclear Periphery | 0.014207 | 0.011963 | 0.019022 | 0.024542 | 0.034985 | 0.025175 | 0.063565 | 0.018373 | 0.033589 | 0.014175 | 0.061035 | 0.021144 |
Nuclear Periphery Foci | 0.008763 | 0.004574 | 0.001678 | 0.001521 | 0.002383 | 0.001128 | 0.001780 | 0.000603 | 0.002811 | 0.003997 | 0.001774 | 0.001016 |
Nucleolus | 0.000128 | 0.000239 | 0.000092 | 0.000212 | 0.000288 | 0.000159 | 0.000156 | 0.000142 | 0.000277 | 0.000226 | 0.000152 | 0.000173 |
Nucleus | 0.000968 | 0.001210 | 0.000474 | 0.000696 | 0.000602 | 0.000397 | 0.000799 | 0.000282 | 0.000627 | 0.001074 | 0.000781 | 0.000468 |
Peroxisomes | 0.000801 | 0.000698 | 0.000016 | 0.000161 | 0.000866 | 0.000082 | 0.001435 | 0.000277 | 0.000861 | 0.000595 | 0.001354 | 0.000225 |
Vacuole | 0.207269* | 0.159202 | 0.235032* | 0.188686 | 0.178622 | 0.140000 | 0.212601* | 0.075297 | 0.180547 | 0.155987 | 0.213875* | 0.126236 |
Vacuole Periphery | 0.006306 | 0.016138 | 0.015130 | 0.013874 | 0.015451 | 0.017620 | 0.012119 | 0.010992 | 0.014836 | 0.016386 | 0.012290 | 0.012287 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.35 | 3.04 | 1.95 | -0.38 | -1.66 | -2.17 | -2.17 | -2.83 | -1.33 | -2.03 | -3.20 | -1.37 | -2.94 | -1.76 | -2.03 |
Bud Neck | 2.01 | 2.00 | 2.49 | 4.28 | 2.66 | -1.45 | -1.90 | -1.26 | 1.31 | -0.14 | -2.96 | 0.91 | 1.00 | 3.77 | 0.19 |
Bud Site | 0.48 | 3.66 | 3.26 | 5.06 | -1.26 | -1.89 | -1.78 | -3.72 | 0.89 | -2.66 | -5.86 | -0.11 | -2.64 | 4.34 | -2.38 |
Cell Periphery | -9.00 | -7.51 | -10.92 | -3.95 | -3.16 | -1.44 | 15.71 | -22.40 | -7.33 | -36.73* | 0.01 | 12.44 | -16.81 | -11.75 | -30.06* |
Cytoplasm | -5.44 | 3.66 | 1.84 | 7.92 | -4.22 | 8.38 | -1.68 | 7.95 | -1.01 | 7.21 | -5.24 | -1.43 | 6.96 | 8.40 | 6.30 |
Cytoplasmic Foci | 3.11 | 3.22 | 3.19 | 4.99 | -2.68 | 0.34 | 3.00 | -0.33 | -0.48 | -0.79 | 2.83 | 3.44 | 3.00 | 0.84 | -0.33 |
Eisosomes | -2.32 | -2.77 | -2.49 | -0.14 | 1.56 | -3.61 | -4.58 | -4.47 | 3.31 | 1.79 | -4.05 | -5.31 | -3.68 | 1.98 | -0.55 |
Endoplasmic Reticulum | 6.16 | 5.57 | 5.92 | -2.17 | 0.16 | -0.15 | -6.01 | 11.62 | 3.33 | 13.87 | 7.46 | -3.18 | 10.30 | 2.17 | 12.09 |
Endosome | 2.93 | 2.96 | 2.98 | 4.51 | 1.33 | -1.50 | 2.30 | 3.44 | 2.54 | 2.32 | 2.59 | 3.13 | 3.62 | 4.66 | 3.37 |
Golgi | 2.24 | 0.97 | 2.86 | 4.89 | 4.26 | -4.58 | -6.15 | -2.25 | 3.45 | 0.96 | -4.74 | -3.72 | -0.19 | 4.41 | 3.31 |
Lipid Particles | 4.26 | 4.22 | 5.58 | 6.71 | 2.34 | -1.45 | -8.74 | 0.34 | 1.50 | 8.52 | -4.28 | -6.05 | 4.30 | 6.96 | 8.91 |
Mitochondria | 0.26 | 1.40 | -0.21 | -0.62 | -1.99 | -1.09 | -0.88 | -0.78 | 0.83 | -0.20 | -2.57 | 0.04 | -1.31 | 0.03 | -1.32 |
Mitotic Spindle | 1.81 | 1.66 | 1.78 | -0.87 | 1.72 | -3.35 | -4.77 | -1.71 | 0.78 | -0.21 | 2.07 | 0.18 | 0.92 | -1.70 | 1.42 |
None | -5.80 | -0.11 | -1.40 | 1.52 | -1.38 | -0.89 | -1.97 | 2.09 | 1.64 | 2.57 | -5.11 | -1.98 | -0.64 | 3.27 | 1.16 |
Nuclear Periphery | 0.80 | -1.76 | -4.76 | -8.50 | -2.09 | 2.66 | -12.24 | 10.63 | 1.86 | 20.49 | 12.97 | -12.37 | 8.96 | -5.76 | 19.58 |
Nuclear Periphery Foci | 2.48 | 4.18 | 4.30 | 5.46 | 0.51 | 6.20 | 3.75 | 12.62 | 3.21 | 10.91 | -2.46 | 5.44 | 9.47 | 6.50 | 6.27 |
Nucleolus | -1.30 | 0.37 | -0.98 | 1.21 | -4.96 | 5.51 | 14.23 | 12.14 | 0.61 | 0.98 | 3.78 | 11.77 | 7.51 | 3.56 | -1.98 |
Nucleus | 0.44 | 2.01 | 1.39 | 1.78 | -0.77 | 1.83 | -2.71 | 4.93 | 0.92 | 6.13 | -3.29 | -1.81 | 1.30 | 3.55 | 2.36 |
Peroxisomes | 0.27 | 3.01 | 2.44 | 2.36 | -6.69 | 10.90 | -3.11 | 5.87 | -2.71 | 6.31 | 1.35 | -2.85 | 8.04 | 1.91 | 6.86 |
Vacuole | 3.04 | -0.82 | 1.48 | -3.91 | 2.97 | 3.66 | -6.54 | 23.25 | 6.24 | 28.88 | 4.14 | -6.42 | 12.05 | 5.27 | 18.36 |
Vacuole Periphery | -6.54 | -2.36 | -5.89 | 1.94 | 0.33 | -0.97 | 5.49 | 7.81 | 2.91 | 1.88 | -1.58 | 4.14 | 4.80 | 4.25 | -0.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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