ORF
Description Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.64 0.66 0.7 0.48 0.62 0.43 0.44 0.44 0.41 0.46 0.25 0.44 0.88 0.94 0.96 0.84 0.89 0.22 0.33 0.22 0.19 0.24 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0.05 0.06 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.47 0.41 0.32 0.58 0.41 0.67 0.66 0.62 0.71 0.73 0.86 0.74 0.07 0.05 0 0.07 0 0.56 0.38 0.52 0.54 0.49 0.49
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0.06 0
Vac/Vac Membrane 0 0.07 0.07 0 0.05 0.06 0 0 0 0 0 0 0.09 0.05 0 0.09 0.06 0.05 0.12 0.05 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 2 4 4 8 25
Bud 0 0 7 7 4 8 8 16 10 21 19 23 0 2 0 1 3 1 2 0 1 3 8
Bud Neck 0 0 1 1 0 1 0 7 0 3 2 1 0 0 0 3 0 1 0 0 0 1 14
Bud Site 0 0 0 1 0 4 3 4 8 8 1 7 0 0 0 0 0
Cell Periphery 1 0 0 2 1 1 2 1 2 0 1 2 0 0 0 2 4 1 0 2 0 3 4
Cytoplasm 195 50 125 78 152 119 224 241 191 238 111 268 177 434 427 218 154 80 36 47 25 75 63
Endoplasmic Reticulum 8 1 0 1 1 1 0 0 1 0 3 0 1 1 2 26 8 8 0 4 0 3 5
Endosome 0 1 3 1 8 1 0 2 0 0 0 0 3 4 2 7 2 17 6 11 6 18 26
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 2 0 1 1 1 0 1 1
Mitochondria 145 31 58 94 100 186 337 346 331 376 379 457 14 25 18 17 7 208 42 110 71 153 192
Nucleus 0 0 1 0 1 3 4 8 2 3 2 7 0 0 1 2 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 1 0 1 1 0 2 1 1 0 0 0 1 0 0 0 1 1 0 0 2
Nucleolus 0 0 1 0 1 0 0 3 1 1 0 2 0 0 2 0 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 1 1 1 1 1 1 1 0 0 0 0 0 3 0 2 0 0 3
SpindlePole 2 0 1 5 7 14 31 26 17 23 10 28 1 0 1 5 4 8 2 8 7 17 13
Vac/Vac Membrane 9 5 12 6 13 17 11 8 6 10 7 7 19 21 5 24 10 19 13 10 5 14 23
Unique Cell Count 306 76 179 162 244 277 511 554 463 516 442 616 202 462 443 258 174 370 111 213 133 312 397
Labelled Cell Count 361 88 209 197 289 357 622 663 572 685 537 803 215 487 460 307 193 370 111 213 133 312 397


Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.7 3.7 3.6 3.7 4.0 2.6 3.0 2.9 2.6 2.6 2.6 4.5 4.2 4.1 4.4 5.0 5.3 4.2 4.3 4.8
Std Deviation (1e-4) 0.4 1.1 1.2 1.0 1.3 6.4 0.9 1.1 1.1 0.8 0.5 1.2 1.6 1.1 0.9 1.4 1.8 1.7 1.7 1.9 1.8
Intensity Change (Log2) -0.06 -0.04 0.1 -0.52 -0.33 -0.39 -0.54 -0.51 -0.54 0.27 0.16 0.12 0.23 0.43 0.51 0.16 0.19 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 1.0 -0.4