Standard name
Human Ortholog
Description Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.93 0.96 0.99 0.97 0.93 0.87 0.76 0.67 0.69 0.65 0.61 0.6 1.0 0.99 0.99 0.98 0.95 0.92 0.88 0.88 0.87 0.93 0.92 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.06 0.11 0.24 0.31 0.29 0.36 0.4 0.5 0.5 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0.14 0 0.08 0 0.06 0 0 0 0 0.05 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 1 0 1 0 0 0 0 0 1 2 4 3 0 1 2
Bud 0 4 4 0 0 3 4 12 5 7 2 6 0 1 0 0 0 0 1 5 1 0 1 4
Bud Neck 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 1 2
Bud Site 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 4 6 6 6 10 8 21 12 10 24 0 5 4 2 9 4 0 1 0 0 0 0
Cytoplasm 280 365 310 259 271 356 305 392 312 248 208 250 129 322 288 202 173 149 272 344 264 101 182 198
Endoplasmic Reticulum 2 6 0 0 1 1 4 9 10 6 7 1 0 0 2 9 10 4 0 0 3 0 1 2
Endosome 0 1 0 1 1 0 0 1 0 2 1 0 0 0 0 2 1 2 3 5 2 0 0 0
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 4 0 0 2 0
Mitochondria 26 6 9 17 32 100 122 172 164 152 169 206 0 0 0 2 3 4 9 8 15 1 2 3
Nucleus 0 0 0 0 1 2 2 6 8 2 1 2 0 0 1 1 0 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 0 4 4 3 5 4 4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2
SpindlePole 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 2 3 1 0 1 0
Vac/Vac Membrane 3 2 0 1 10 15 25 81 15 29 14 24 0 3 1 4 10 10 5 2 2 1 0 6
Unique Cell Count 301 382 314 266 291 410 399 589 451 379 341 414 129 325 291 207 183 162 309 389 303 109 198 230
Labelled Cell Count 312 390 327 285 323 484 477 686 541 464 416 518 129 331 296 225 206 176 309 389 303 109 198 230


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.9 4.2 4.3 5.3 5.4 5.9 5.7 6.3 6.1 6.1 6.3 6.3 8.2 8.7 7.1 7.9 7.6 4.2 4.6 5.2
Std Deviation (1e-4) 0.7 1.3 1.1 1.5 1.9 2.0 2.6 2.0 2.4 2.5 2.2 2.6 2.6 3.1 3.2 2.8 2.7 2.6 1.3 1.5 1.4
Intensity Change (Log2) – – – 0.03 0.32 0.35 0.48 0.43 0.58 0.52 0.53 0.56 0.56 0.95 1.04 0.74 0.9 0.83 -0.01 0.12 0.3

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.8 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.2 0 2.6 1.7 1.5 3.1 0 0 0 0 0 0
Cytoplasm -1.2 -3.5 -5.8 -8.6 -11.1 -10.3 -11.0 -11.9 -12.2 1.3 0.4 0.3 -1.0 -2.7 -3.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.0 8.0 9.5 9.4 10.9 11.6 13.4 13.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.4 4.5 6.9 3.3 5.0 3.6 4.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2922 4.2406 3.7741 2.9508 2.8944 3.9037 5.6449 5.2361 6.1079 5.9671 6.7342 5.4954 6.2988 6.8667 6.2256 6.1958 6.5673 7.3556
Actin 0.0366 0.0008 0.0033 0.0103 0.0028 0.0065 0.0508 0.0031 0.0213 0.0126 0.0325 0.0189 0.0132 0.0034 0.0362 0.0042 0.0372 0.0106
Bud 0.0037 0.0064 0.0054 0.0004 0.0042 0.0004 0.0017 0.0019 0.0034 0.0007 0.0018 0.005 0.0012 0.0019 0.0059 0.0025 0.0119 0.0031
Bud Neck 0.0124 0.0002 0.0003 0.0007 0.0007 0.001 0.0019 0.0004 0.0008 0.0015 0.0024 0.0012 0.0033 0.0003 0.0055 0.0004 0.0014 0.0018
Bud Periphery 0.0086 0.0045 0.005 0.0006 0.0067 0.0007 0.0027 0.0034 0.0055 0.0008 0.0033 0.0025 0.0014 0.0012 0.0069 0.0041 0.0179 0.0064
Bud Site 0.0046 0.0044 0.0065 0.0016 0.0005 0.0006 0.0047 0.0056 0.022 0.0021 0.0025 0.0006 0.0023 0.0082 0.0208 0.0015 0.0025 0.0007
Cell Periphery 0.0009 0.0006 0.0004 0.0003 0.0001 0.0001 0.0004 0.0005 0.0007 0.0003 0.0002 0.0002 0.0002 0.0003 0.0004 0.0003 0.0003 0.0001
Cytoplasm 0.6023 0.7484 0.6265 0.6534 0.6091 0.7462 0.6244 0.7032 0.7117 0.5928 0.6631 0.7485 0.6785 0.8104 0.6508 0.609 0.6075 0.763
Cytoplasmic Foci 0.0235 0.0076 0.0125 0.0484 0.0189 0.0138 0.039 0.0065 0.0114 0.0306 0.0493 0.019 0.026 0.0128 0.0178 0.0105 0.0236 0.0118
Eisosomes 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0003 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0 0.0003 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0062 0.0025 0.0031 0.0034 0.003 0.0024 0.013 0.0019 0.0042 0.0042 0.0049 0.0032 0.008 0.0022 0.0057 0.0028 0.0071 0.0049
Endosome 0.0141 0.0029 0.0032 0.0131 0.0056 0.0073 0.0263 0.0021 0.0187 0.0312 0.0112 0.0131 0.0186 0.0069 0.0081 0.017 0.0107 0.0119
Golgi 0.0061 0.0003 0.0008 0.0026 0.0005 0.0017 0.0158 0.0005 0.0043 0.0259 0.004 0.0066 0.0046 0.0047 0.0049 0.0033 0.0037 0.0035
Lipid Particles 0.0063 0.0001 0.0012 0.0028 0.0003 0.0041 0.0115 0.0002 0.003 0.0131 0.0243 0.0023 0.0096 0.0009 0.0033 0.0055 0.0041 0.0007
Mitochondria 0.002 0.0005 0.0058 0.0014 0.0014 0.0029 0.0063 0.0005 0.012 0.0131 0.0016 0.0035 0.002 0.0005 0.0095 0.0014 0.0084 0.0021
None 0.2469 0.2134 0.3165 0.2437 0.3365 0.2011 0.1557 0.2621 0.1655 0.2094 0.1683 0.1626 0.1878 0.1409 0.2054 0.316 0.2268 0.166
Nuclear Periphery 0.0049 0.0009 0.0012 0.0007 0.0013 0.0025 0.0097 0.0007 0.0026 0.0012 0.001 0.0012 0.0125 0.0006 0.0024 0.0036 0.0066 0.0026
Nucleolus 0.0009 0.0002 0.0003 0.0001 0.0001 0.0007 0.0009 0.0002 0.0002 0.0003 0.0003 0.0002 0.0007 0.0001 0.0002 0.0004 0.0006 0.0007
Nucleus 0.004 0.0022 0.0019 0.0013 0.002 0.0018 0.0093 0.0016 0.0018 0.0013 0.0013 0.0014 0.0036 0.0017 0.0022 0.0026 0.0106 0.003
Peroxisomes 0.0074 0.0002 0.0011 0.0121 0.0014 0.003 0.0107 0.002 0.0059 0.0538 0.0134 0.0051 0.0127 0.0006 0.0091 0.0033 0.0121 0.0018
Punctate Nuclear 0.0037 0.0007 0.0025 0.0007 0.0015 0.0019 0.0096 0.0015 0.0014 0.0013 0.0114 0.0032 0.0104 0.0007 0.002 0.0087 0.0037 0.0034
Vacuole 0.0038 0.0029 0.0019 0.002 0.0027 0.0011 0.0037 0.0017 0.0025 0.0029 0.0026 0.0012 0.0023 0.0014 0.0022 0.0022 0.0024 0.0014
Vacuole Periphery 0.0007 0.0003 0.0003 0.0004 0.0008 0.0003 0.0016 0.0001 0.0008 0.0009 0.0005 0.0003 0.0009 0.0001 0.0005 0.0006 0.0007 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.175 21.0007 32.1408 65.197 48.5437 60.8994 37.1591 60.4605 103.0956 64.1658
Translational Efficiency 1.7321 1.3143 1.3152 1.7284 1.508 1.8653 1.6803 1.4387 1.5448 1.5594

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
551 1093 122 1904 258 1592 1038 475 809 2685 1160 2379

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 668.16 688.69 1023.99 1035.52 900.99 698.38 1264.33 969.80 742.41 694.44 1239.05 1022.40
Standard Deviation 90.02 98.81 208.02 261.19 266.72 103.85 370.81 279.93 199.95 101.94 364.72 266.34
Intensity Change Log 2 — 0.043661 0.615936 0.632090 — -0.367499 0.488790 0.106176 — -0.177943 0.544306 0.353849

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000053 0.000434 0.000375 0.002834 0.000227 0.000556 0.000198 0.000541 0.000108 0.000507 0.000217 0.002376
Bud Neck 0.008688 0.035731 0.001612 0.010940 0.000940 0.048488 0.001648 0.004833 0.006217 0.043295 0.001644 0.009721
Bud Site 0.003815 0.023150 0.005423 0.045020 0.004329 0.030516 0.007280 0.031580 0.003979 0.027518 0.007084 0.042337
Cell Periphery 0.000145 0.000148 0.000280 0.000191 0.000171 0.000146 0.000114 0.000266 0.000153 0.000147 0.000131 0.000206
Cytoplasm 0.590827 0.396379 0.450541 0.654680 0.785799 0.385428 0.779828 0.584152 0.653006 0.389886 0.745196 0.640598
Cytoplasmic Foci 0.176248 0.263423 0.069867 0.016540 0.076723 0.241820 0.011263 0.038353 0.144508 0.250614 0.017426 0.020895
Eisosomes 0.000129 0.000157 0.000107 0.000020 0.000035 0.000130 0.000011 0.000030 0.000099 0.000141 0.000021 0.000022
Endoplasmic Reticulum 0.000469 0.000759 0.002600 0.000885 0.000585 0.000714 0.000604 0.002370 0.000506 0.000733 0.000814 0.001182
Endosome 0.007606 0.035286 0.014729 0.004943 0.003719 0.039772 0.001910 0.010597 0.006366 0.037946 0.003258 0.006072
Golgi 0.000747 0.006202 0.000280 0.006548 0.000440 0.008708 0.000430 0.009734 0.000649 0.007688 0.000414 0.007184
Lipid Particles 0.004694 0.006559 0.004612 0.001341 0.001465 0.005641 0.000762 0.001668 0.003664 0.006015 0.001166 0.001406
Mitochondria 0.002536 0.013537 0.000194 0.003630 0.000220 0.018958 0.000183 0.007539 0.001798 0.016751 0.000184 0.004411
Mitotic Spindle 0.000516 0.004679 0.001305 0.085018 0.000498 0.019785 0.015685 0.046757 0.000511 0.013636 0.014173 0.077379
None 0.003936 0.004080 0.002396 0.006706 0.008349 0.003243 0.006771 0.006815 0.005343 0.003584 0.006311 0.006728
Nuclear Periphery 0.000369 0.000359 0.000994 0.000710 0.000158 0.000377 0.000473 0.000899 0.000302 0.000370 0.000528 0.000748
Nuclear Periphery Foci 0.000367 0.000550 0.006670 0.001354 0.003159 0.000391 0.003483 0.002690 0.001258 0.000456 0.003818 0.001621
Nucleolus 0.000813 0.002109 0.001031 0.000514 0.000311 0.002162 0.000152 0.001871 0.000653 0.002141 0.000244 0.000785
Nucleus 0.152244 0.073656 0.182607 0.083407 0.078867 0.058201 0.098105 0.076113 0.128843 0.064492 0.106993 0.081951
Peroxisomes 0.002275 0.017840 0.000670 0.002540 0.000319 0.023188 0.000090 0.006423 0.001651 0.021011 0.000151 0.003315
Vacuole 0.043059 0.111366 0.253067 0.069715 0.033321 0.108089 0.070588 0.159086 0.039953 0.109423 0.089780 0.087559
Vacuole Periphery 0.000463 0.003595 0.000638 0.002463 0.000366 0.003686 0.000423 0.007682 0.000432 0.003649 0.000445 0.003505

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -11.03 -6.57 -3.72 -2.21 -2.31 -3.16 -0.09 -1.83 1.60 -2.78 -8.53 -5.11 -3.75 -1.83 -3.00
Bud Neck -10.13 6.33 1.59 11.58 -7.78 -19.19 -5.54 -6.48 17.73 -3.04 -19.20 5.08 0.08 20.12 -7.16
Bud Site -9.08 -2.10 -9.89 0.31 -8.75 -12.47 -2.50 -5.90 2.28 -4.89 -15.23 -3.02 -11.50 2.49 -8.95
Cell Periphery -0.04 -3.14 1.61 1.94 4.07 1.59 2.04 -1.00 -1.76 -1.95 0.85 0.61 0.96 0.36 0.49
Cytoplasm 14.61 6.39 -0.25 -18.50 -6.84 25.11 6.52 12.84 -8.92 9.41 25.05 0.12 6.67 -23.63 7.22
Cytoplasmic Foci -8.98 13.05 23.15 36.03 5.88 -17.88 8.74 5.94 32.94 -5.28 -15.06 22.91 22.67 52.12 -1.11
Eisosomes -2.33 2.30 12.34 17.63 7.88 -12.36 4.39 1.84 18.07 -6.82 -5.18 11.66 12.17 26.11 0.87
Endoplasmic Reticulum -2.68 -6.61 -2.61 0.31 5.89 -0.49 -0.26 -1.64 -1.30 -1.58 -2.05 -3.98 -2.98 -0.68 0.68
Endosome -11.19 -2.24 3.96 16.26 6.94 -19.21 4.23 -1.22 16.88 -3.57 -18.93 3.78 3.74 25.46 0.06
Golgi -9.01 3.75 -1.67 5.21 -2.55 -9.96 1.21 -1.27 2.70 -1.33 -12.67 3.08 -2.16 6.82 -2.72
Lipid Particles -2.49 0.80 5.30 9.44 4.69 -7.85 1.48 -1.11 5.59 -2.01 -4.46 5.51 4.71 11.05 -0.81
Mitochondria -4.49 1.87 1.00 5.94 -5.57 -11.12 0.85 -1.63 7.75 -1.66 -9.47 1.89 0.36 11.39 -4.32
Mitotic Spindle -3.95 -1.48 -12.48 -10.81 -12.15 -7.59 -7.57 -5.47 -1.60 -2.29 -8.27 -7.54 -13.63 -7.79 -7.12
None -0.14 1.40 -1.53 -2.17 -8.03 2.58 1.57 1.92 -1.36 1.08 1.45 0.29 -0.35 -3.59 -1.62
Nuclear Periphery 0.04 -6.64 -7.58 -9.58 0.97 -7.04 -15.55 -8.34 -5.48 -1.75 -1.56 -8.56 -10.43 -11.28 -3.99
Nuclear Periphery Foci -2.01 -7.29 -8.35 -6.66 5.63 5.37 -0.97 1.42 -6.77 3.32 4.35 -7.94 -2.52 -9.78 6.78
Nucleolus -3.30 0.17 1.48 5.73 1.96 -5.28 2.71 -1.23 3.00 -1.58 -5.03 2.22 0.88 6.61 -2.77
Nucleus 10.05 -1.22 4.22 -9.59 2.96 2.40 -3.67 0.29 -2.96 5.47 10.82 1.29 2.83 -13.22 1.80
Peroxisomes -10.18 2.15 1.52 12.80 -1.41 -13.74 5.44 -1.40 9.75 -1.58 -15.45 2.78 0.67 16.83 -3.04
Vacuole -13.20 -10.50 -22.93 -9.44 4.90 -11.21 -13.63 -17.24 -12.62 -8.67 -18.30 -18.15 -28.21 -15.35 -3.53
Vacuole Periphery -7.52 -0.25 -1.56 6.36 -1.23 -10.82 1.14 -2.07 1.32 -2.19 -12.93 1.73 -2.64 7.56 -3.15
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Emi2

Emi2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Emi2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available