ORF
Human Ortholog
Description Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.95 0.95 0.94 0.84 – 0.67 0.66 0.61 0.55 0.56 1.0 0.99 0.99 0.96 0.96 0.94 0.9 0.92 0.88 0.91 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.2 0.07 0.28 – 0.42 0.51 0.55 0.59 0.6 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 – 0.07 0.06 0.06 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 3 1 0 1 3
Bud 0 0 7 1 1 0 – 5 6 10 11 10 0 1 0 0 1 0 4 3 2 1 5 11
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 3
Bud Site 0 0 0 0 0 0 – 0 3 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 1 1 2 8 – 4 1 1 3 5 0 0 1 5 1 2 0 0 1 0 0 0
Cytoplasm 310 376 271 227 253 335 – 377 313 345 209 298 289 372 322 166 175 146 286 361 257 137 197 240
Endoplasmic Reticulum 2 0 1 0 0 1 – 1 1 2 2 6 1 1 1 4 5 5 0 0 1 0 2 2
Endosome 0 2 2 1 1 1 – 11 1 1 1 0 1 1 0 1 2 2 4 3 3 3 1 2
Golgi 0 2 0 0 0 0 – 0 0 0 0 0 0 1 0 0 2 1 1 3 0 1 5 2
Mitochondria 4 3 24 47 19 112 – 236 238 309 225 319 0 0 0 3 2 0 3 4 8 2 2 2
Nucleus 0 0 0 0 0 0 – 8 3 10 3 13 0 0 1 1 2 7 1 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 – 5 4 4 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 0 0 – 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 1 0 – 1 0 1 1 0 0 0 0 1 0 0 5 2 3 1 1 7
Vac/Vac Membrane 2 2 2 1 7 19 – 42 30 35 17 24 0 0 1 6 2 10 2 4 2 1 1 2
Unique Cell Count 317 384 286 238 268 398 562 471 562 379 529 290 375 324 173 183 156 318 391 293 152 226 283
Labelled Cell Count 318 387 309 279 284 476 690 601 719 472 680 291 376 326 187 192 173 318 391 293 152 226 283


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.8 4.3 4.6 5.3 4.7 – 4.3 3.9 4.0 3.9 3.8 5.8 5.8 5.7 8.1 7.2 7.3 6.0 6.5 6.3
Std Deviation (1e-4) 0.7 0.9 1.6 1.5 1.9 3.4 – 1.2 1.4 0.9 1.3 1.4 1.0 1.0 1.3 2.3 1.7 1.5 1.3 1.9 1.5
Intensity Change (Log2) – – – 0.12 0.31 0.15 – 0.01 -0.12 -0.08 -0.14 -0.15 0.45 0.45 0.43 0.93 0.76 0.77 0.5 0.61 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.2 -4.3 – -9.0 -9.0 -10.3 -11.3 -11.4 3.6 3.5 3.5 0.6 0.4 -0.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -0.6 0 – 0 0 0 0 0 -5.0 -5.7 -5.3 -2.9 -3.4 -3.7
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.0 – 4.2 3.8 3.7 2.9 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6916 0.34 0.1861 -0.2396 0.1546 0.1449 0.5764 0.4575 0.3362 0.1648 0.0868 0.3824 2.2278 1.9238 1.8573 2.1101 1.7597 1.5971
Actin 0.0228 0.001 0.0072 0.0093 0.0072 0.0133 0.0056 0.0024 0.0101 0.0318 0.0022 0.0107 0.0002 0.0003 0.0005 0.0177 0.0004 0.0006
Bud 0.0005 0.0001 0.0002 0.0003 0.001 0 0.0004 0.001 0.0012 0.0006 0.0001 0.0001 0 0.0003 0.0003 0.0008 0.0008 0.0001
Bud Neck 0.0029 0.0001 0.0004 0.0002 0.0091 0.0003 0.0085 0.0002 0.0005 0.0012 0.0002 0.0006 0 0.0002 0.0006 0.0127 0.0002 0.0095
Bud Periphery 0.0008 0.0001 0.0005 0.0006 0.0012 0 0.0006 0.0009 0.0014 0.0009 0.0001 0.0001 0 0.0003 0.0003 0.0012 0.0006 0.0001
Bud Site 0.0045 0.001 0.0022 0.0007 0.025 0 0.0122 0.0138 0.0045 0.001 0.0002 0.0002 0.0001 0.0041 0.0033 0.0182 0.0001 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0008 0.0001 0.0002 0.0001 0 0 0.0001 0.0001 0.0001 0.0005 0 0
Cytoplasm 0.5157 0.4859 0.5685 0.4506 0.3332 0.6477 0.5352 0.53 0.538 0.4513 0.5475 0.5878 0.5805 0.4748 0.5661 0.4911 0.4451 0.6939
Cytoplasmic Foci 0.0407 0.0099 0.0165 0.0102 0.0602 0.0071 0.0211 0.0075 0.0386 0.0223 0.018 0.0196 0.0061 0.004 0.0062 0.0082 0.0058 0.0062
Eisosomes 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0005 0.0001 0
Endoplasmic Reticulum 0.0032 0.0032 0.0056 0.0039 0.0032 0.0023 0.0042 0.0015 0.0035 0.0017 0.0019 0.0012 0.0061 0.001 0.002 0.002 0.0018 0.0022
Endosome 0.0101 0.0036 0.0067 0.0129 0.0346 0.0021 0.023 0.0042 0.007 0.0026 0.011 0.0032 0.0013 0.0007 0.0012 0.0033 0.0011 0.0015
Golgi 0.004 0.0003 0.0008 0.0066 0.0044 0.001 0.0077 0.0003 0.003 0.0027 0.0082 0.0006 0.0001 0.0001 0.0001 0.0007 0.0001 0.0001
Lipid Particles 0.0106 0.0004 0.0009 0.0287 0.0192 0.0022 0.0111 0.0005 0.0032 0.0028 0.0046 0.0004 0.0001 0.0001 0.0003 0.0004 0.0001 0.0001
Mitochondria 0.0025 0.0001 0.0015 0.0355 0.0118 0.0002 0.0034 0.0058 0.0048 0.0013 0.0019 0.0001 0.0001 0.0001 0.001 0.0007 0.0002 0.0002
None 0.3349 0.4894 0.3753 0.4213 0.4182 0.3182 0.3336 0.4229 0.3635 0.4591 0.3896 0.3685 0.4017 0.5108 0.4094 0.418 0.5377 0.2799
Nuclear Periphery 0.0076 0.0008 0.005 0.0022 0.0071 0.0005 0.0032 0.0007 0.0021 0.0012 0.0007 0.0006 0.0009 0.0004 0.0007 0.0021 0.0009 0.0007
Nucleolus 0.0007 0.0001 0.0002 0.0004 0.0013 0 0.0016 0.0004 0.0004 0.0002 0 0 0 0.0001 0.0017 0.0006 0.0001 0
Nucleus 0.0049 0.0014 0.0033 0.0017 0.004 0.0013 0.0048 0.0028 0.0034 0.0024 0.0016 0.0025 0.0013 0.0014 0.004 0.0177 0.0022 0.0032
Peroxisomes 0.0227 0.0001 0.0002 0.01 0.0317 0.0026 0.0137 0.0006 0.0101 0.0136 0.0102 0.0009 0.0001 0.0001 0.0005 0.0006 0.0001 0.0002
Punctate Nuclear 0.0078 0.0013 0.003 0.0031 0.0223 0.0006 0.0029 0.0012 0.0028 0.0024 0.0006 0.0023 0.0006 0.0007 0.0012 0.0018 0.0022 0.001
Vacuole 0.0015 0.0009 0.0012 0.0011 0.0035 0.0004 0.0051 0.0029 0.0012 0.0005 0.0008 0.0005 0.0005 0.0004 0.0005 0.0011 0.0005 0.0005
Vacuole Periphery 0.001 0.0001 0.0005 0.0006 0.0016 0.0001 0.0008 0.0002 0.0003 0.0002 0.0003 0.0001 0 0 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.9094 5.5727 6.2028 6.7208 18.1857 12.4609 12.6778 7.6059 3.9702 10.4399
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
166 140 947 1380 1065 1211 612 1516 1231 1351 1559 2896

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 652.37 798.72 866.46 834.58 695.93 772.06 939.37 845.43 690.06 774.82 895.08 840.26
Standard Deviation 105.78 109.66 114.44 120.44 85.48 102.50 122.47 112.64 89.73 103.58 122.93 116.55
Intensity Change Log 2 — 0.291999 0.409443 0.355360 — 0.149771 0.432751 0.280743 — 0.220340 0.421521 0.317328

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000129 0.000697 0.000393 0.000361 0.000111 0.000421 0.000193 0.000831 0.000113 0.000450 0.000314 0.000607
Bud Neck 0.023359 0.029552 0.002914 0.006449 0.010841 0.027467 0.005427 0.007913 0.012529 0.027683 0.003901 0.007216
Bud Site 0.002986 0.017265 0.006325 0.015949 0.005538 0.015698 0.005661 0.024605 0.005193 0.015860 0.006064 0.020480
Cell Periphery 0.000155 0.000190 0.000132 0.000252 0.000127 0.000135 0.000144 0.000110 0.000131 0.000141 0.000137 0.000178
Cytoplasm 0.431585* 0.394193* 0.419121* 0.526848* 0.512037* 0.465880* 0.475951* 0.528265* 0.501188* 0.458452* 0.441430* 0.527590*
Cytoplasmic Foci 0.260929* 0.219039* 0.010605 0.018713 0.217053* 0.227654* 0.021070 0.024669 0.222970* 0.226761* 0.014713 0.021831
Eisosomes 0.000185 0.000155 0.000035 0.000043 0.000139 0.000118 0.000055 0.000041 0.000145 0.000122 0.000043 0.000042
Endoplasmic Reticulum 0.000765 0.001852 0.003796 0.002456 0.000691 0.000567 0.002260 0.001393 0.000701 0.000700 0.003193 0.001900
Endosome 0.016239 0.023473 0.002091 0.005600 0.011539 0.023003 0.003354 0.009859 0.012173 0.023052 0.002587 0.007829
Golgi 0.000870 0.001867 0.000094 0.002382 0.001171 0.003447 0.000121 0.003877 0.001130 0.003283 0.000105 0.003165
Lipid Particles 0.006428 0.003562 0.001068 0.001566 0.006872 0.004239 0.001931 0.000989 0.006812 0.004168 0.001407 0.001264
Mitochondria 0.001519 0.008112 0.000417 0.006731 0.004344 0.006947 0.000922 0.005264 0.003963 0.007068 0.000616 0.005963
Mitotic Spindle 0.000100 0.005243 0.002878 0.026469 0.000772 0.006248 0.007880 0.044979 0.000681 0.006144 0.004841 0.036159
None 0.002117 0.001215 0.004870 0.008817 0.001812 0.002502 0.003856 0.005982 0.001853 0.002369 0.004472 0.007333
Nuclear Periphery 0.000437 0.000470 0.001101 0.001736 0.000380 0.000320 0.001402 0.001194 0.000388 0.000336 0.001219 0.001452
Nuclear Periphery Foci 0.000479 0.001120 0.001839 0.002052 0.000507 0.000409 0.001456 0.001150 0.000503 0.000483 0.001689 0.001579
Nucleolus 0.000730 0.001176 0.000343 0.000728 0.000710 0.001170 0.000348 0.000699 0.000713 0.001170 0.000345 0.000713
Nucleus 0.175043* 0.126451 0.488775* 0.228534* 0.148302* 0.114133 0.364590* 0.204890* 0.151908* 0.115409 0.440025* 0.216157*
Peroxisomes 0.004884 0.019870 0.000661 0.001206 0.003535 0.010542 0.001093 0.005821 0.003717 0.011508 0.000831 0.003622
Vacuole 0.070429 0.140796 0.052120 0.141385 0.072850 0.087411 0.102131 0.123566 0.072524 0.092943 0.071753 0.132057
Vacuole Periphery 0.000631 0.003702 0.000421 0.001723 0.000671 0.001691 0.000153 0.003903 0.000666 0.001899 0.000315 0.002864

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.01 -4.57 -4.32 2.62 1.64 -1.99 -6.55 -4.56 -0.20 -2.21 -2.42 -6.53 -6.41 0.38 -0.15
Bud Neck -0.48 3.17 2.87 3.07 -1.93 -6.54 4.50 4.23 8.72 -1.34 -5.91 6.76 5.47 9.51 -2.55
Bud Site -4.31 -1.98 -5.99 1.67 -3.07 -4.71 0.77 -3.80 0.97 -4.94 -5.53 0.05 -4.57 1.84 -5.43
Cell Periphery -0.89 0.74 0.42 1.18 -0.11 -0.47 -0.24 5.55 4.62 6.11 -0.64 -0.70 1.44 1.78 2.02
Cytoplasm 1.50 2.82 1.16 -0.86 -3.09 3.91 11.34 9.15 4.85 -4.31 3.93 13.45 10.05 5.17 -5.23
Cytoplasmic Foci 2.28 16.32 16.01 13.08 -4.24 -1.23 33.26 33.34 34.27 -1.41 -0.38 37.72 37.06 36.82 -4.07
Eisosomes 1.25 7.94 7.36 4.16 -3.73 1.36 4.82 5.98 13.16 4.14 1.74 6.98 7.07 13.28 0.17
Endoplasmic Reticulum -2.97 -12.70 -9.04 -2.01 6.44 1.05 -11.62 -9.25 -14.01 7.69 -0.26 -17.77 -13.44 -15.50 10.24
Endosome -1.15 4.38 4.04 4.19 -2.20 -6.05 7.78 5.84 11.19 -1.61 -6.01 9.35 7.72 12.74 -2.68
Golgi -1.66 2.65 0.03 1.16 -1.21 -4.13 4.09 -0.24 3.52 -3.11 -4.33 4.48 0.13 3.88 -2.73
Lipid Particles 2.89 6.56 6.26 3.88 -1.56 3.15 6.17 7.28 10.76 4.72 3.58 7.58 7.94 10.66 2.27
Mitochondria -1.19 2.41 -1.18 0.95 -2.58 -1.29 2.37 1.27 3.29 -2.32 -1.64 2.56 1.06 3.34 -3.54
Mitotic Spindle -1.85 -3.25 -7.77 -4.27 -6.05 -3.52 -2.04 -8.24 -5.67 -6.00 -3.83 -3.16 -11.07 -6.96 -8.70
None 1.14 -2.24 -2.52 -4.49 -1.02 -1.10 -4.36 -6.23 -3.55 -2.84 -0.90 -5.56 -6.00 -3.92 -2.18
Nuclear Periphery -1.06 -12.44 -16.97 -15.49 -8.98 1.77 -19.74 -19.08 -20.02 -0.08 1.44 -24.34 -26.25 -27.35 -7.32
Nuclear Periphery Foci -2.51 -7.58 -6.38 -1.46 1.38 1.39 -5.20 -3.59 -5.19 3.29 0.21 -9.14 -7.09 -7.62 3.61
Nucleolus -1.70 3.97 1.76 2.51 -1.83 -3.07 4.03 1.78 4.65 -2.21 -3.38 4.72 2.19 5.31 -2.84
Nucleus 2.38 -18.93 -5.05 -8.50 22.77 5.07 -22.57 -16.03 -21.17 11.60 5.69 -37.67 -17.90 -25.01 24.81
Peroxisomes -2.55 4.59 4.63 3.42 -0.20 -6.53 6.63 2.02 7.11 -2.01 -6.90 7.82 4.70 9.15 -1.89
Vacuole -5.19 -1.06 -17.75 -5.87 -21.21 -2.62 -10.15 -21.07 -19.08 -4.80 -4.37 -7.71 -29.50 -24.63 -18.82
Vacuole Periphery -1.26 1.72 -0.23 1.23 -1.45 -3.63 5.77 -1.48 0.70 -2.76 -3.56 3.44 -1.38 2.05 -2.94
ORF
Human Ortholog
Description Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
Localization
Cell Percentages cytoplasm (24%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YJR146W

YJR146W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjr146w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available