Standard name
Description Ferulic acid decarboxylase; involved in the decarboxylation of aromatic carboxylic acids such as phenylacrylic (cinnamic) acid, ferulic acid and coumaric acid and formation of the corresponding vinyl derivatives; overexpression of both Pad1p and Fdc1p increases decarboxylase activity due to the Pad1p-catalyzed formation of a diffusible cofactor required for Fdc1p activity; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm in an HTP study

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.98 0.96 0.88 0.84 0.76 0.72 0.67 0.67 0.62 0.98 0.99 0.98 0.92 0.87 0.87 0.9 0.9 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0.17 0.21 0.31 0.39 0.35 0.46 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0.06 0.08 0.06 0.06 0.08 0.08 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 1 1 1 0 1 1
Bud 0 4 0 0 0 0 3 4 3 5 3 3 1 1 0 3 16 3 0 3 15
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Cell Periphery 0 2 2 0 1 3 4 6 7 5 4 10 1 2 4 0 0 0 0 0 0
Cytoplasm 192 392 219 140 237 323 387 355 291 319 206 197 187 393 471 170 352 206 88 213 229
Endoplasmic Reticulum 0 0 2 0 1 1 1 7 12 14 7 0 0 1 2 0 1 4 2 2 4
Endosome 0 0 0 0 0 0 2 2 0 0 0 0 0 2 1 0 4 2 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Mitochondria 8 6 2 4 7 55 79 97 124 187 109 147 1 1 1 2 4 4 0 2 3
Nucleus 0 2 4 1 0 0 1 9 10 6 4 7 3 1 4 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 1 1 0 5 3 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 0 1 1 0 0 0 0 1 8 0 0 0 1
Vac/Vac Membrane 0 2 0 4 9 24 30 36 23 29 24 25 0 3 3 0 6 7 3 6 9
Unique Cell Count 194 400 226 143 247 367 462 469 404 476 308 318 190 398 480 186 405 238 98 237 277
Labelled Cell Count 200 408 230 149 255 406 511 521 471 573 362 391 193 405 486 186 405 238 98 237 277


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.3 7.1 7.5 7.8 6.9 6.4 6.5 6.1 5.7 5.9 5.5 8.8 9.0 8.8 7.5 8.3 9.0
Std Deviation (1e-4) 2.0 2.5 2.1 2.1 2.5 2.2 2.4 2.1 2.0 2.0 2.0 2.0 2.6 2.6 2.7 2.5 2.6 2.7
Intensity Change (Log2) 0.08 0.13 -0.05 -0.15 -0.13 -0.21 -0.31 -0.27 -0.36 0.31 0.35 0.3 0.08 0.23 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 -0.6 -3.8 -5.0 -6.9 -7.6 -8.7 -8.5 -9.5 1.0 1.6 1.0
Endoplasmic Reticulum 0 0 0 0 0 1.7 1.7 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.7 6.2 7.0 9.0 10.7 9.7 11.7 0 0 0
Nucleus 0 0 0 0 0 0.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.9 3.9 4.3 3.7 3.8 4.3 4.3 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fdc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available