Standard name
Human Ortholog
Description Non-catalytic subunit of the NatC N-terminal acetyltransferase; required for replication of dsRNA virus; expression is glucose-repressible; human NatC ortholog, Naa35, requires co-expression of the human catalytic subunit, Naa30, to functionally complement the null allele
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.09 0.06 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 1.0 0.99 0.96 0.96 0.96 0.85 – 0.73 0.82 0.98 0.98 0.99 0.97 0.96 0.9 0.95 0.96 0.91 0.79 0.83 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.07 0 0.36 – 0.49 0.3 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0.07 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 0 – 0 0 0 0 0 1 1 0 0 0 0 0 1 3
Bud 0 1 3 2 2 2 3 2 6 – 7 4 7 4 3 1 0 0 1 0 0 0 8 4
Bud Neck 0 0 3 0 2 2 10 9 1 – 2 1 0 1 0 0 0 0 0 0 2 1 3 2
Bud Site 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 17 2 5 7 4 5 7 – 3 1 3 7 3 29 22 11 0 0 0 0 0 2
Cytoplasm 176 83 324 284 380 428 588 598 347 – 212 65 441 530 644 359 239 179 171 100 305 126 385 475
Endoplasmic Reticulum 1 1 0 0 0 0 3 0 0 – 1 0 2 3 0 17 13 4 0 0 1 5 10 10
Endosome 0 0 0 0 0 0 0 1 0 – 1 0 1 0 0 1 0 1 1 0 1 1 2 3
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 3 0 1 0 0 0 1 1 2
Mitochondria 4 2 0 3 5 39 41 28 146 – 142 24 3 10 8 0 2 4 1 0 7 8 23 20
Nucleus 0 0 4 3 2 0 4 4 2 – 1 0 0 1 0 0 4 2 0 0 2 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 4 0 – 0 1 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 0 0 0 0 2 1 2 5 3 – 5 1 0 2 1 7 2 13 1 1 7 10 17 15
Unique Cell Count 179 85 326 285 385 444 612 626 407 292 79 448 539 649 370 249 198 181 105 336 160 465 549
Labelled Cell Count 183 87 351 294 399 480 655 657 513 374 97 458 559 661 418 283 215 181 105 336 160 465 549


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.1 5.7 5.2 5.8 4.6 4.6 4.9 3.9 – 3.7 3.7 5.8 5.3 5.6 9.1 9.0 8.8 5.0 5.0 5.6
Std Deviation (1e-4) 0.7 0.7 0.8 1.2 1.2 1.0 1.0 1.1 0.8 – 0.9 0.5 1.0 1.1 1.0 1.6 1.2 1.2 1.2 0.9 1.1
Intensity Change (Log2) – – – -0.13 0.01 -0.33 -0.31 -0.23 -0.57 – -0.62 -0.62 0.02 -0.11 -0.03 0.67 0.66 0.62 -0.19 -0.2 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -3.2 -3.0 0 0 -4.3 0 – 0 0 -3.9 -3.4 -4.9 1.4 1.7 0.2
Cytoplasm 0.5 -0.9 -2.7 -2.9 -3.2 -6.8 – -9.8 -7.0 -1.2 -1.3 -0.3 -2.3 -2.8 -5.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.9 4.2 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.9 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5066 1.0492 0.7172 0.7634 0.3504 0.8489 -0.2767 0.4869 0.5508 0.3493 -0.193 0.294 0.9196 1.6255 1.2765 1.6424 1.1288 1.1714
Actin 0.0177 0.0063 0.0101 0.0044 0.0692 0.0034 0.0425 0.0001 0.0229 0.0035 0.0111 0.0013 0.0266 0.0021 0.024 0.0041 0.0328 0.0001
Bud 0.0008 0.0007 0.0009 0.0069 0.001 0.0004 0.0015 0.0001 0.0011 0.0002 0.0088 0.0002 0.0006 0.0009 0.0008 0.0001 0.0013 0
Bud Neck 0.0057 0.0011 0.0006 0.0009 0.0007 0.0012 0.0122 0.0001 0.0011 0.0004 0.0004 0.0005 0.0051 0.0002 0.0004 0.0003 0.0005 0.0006
Bud Periphery 0.0014 0.0008 0.0007 0.0114 0.0009 0.0005 0.002 0 0.0013 0.0003 0.0252 0.0002 0.0007 0.0006 0.0007 0.0001 0.0016 0
Bud Site 0.0022 0.0086 0.0172 0.0011 0.0058 0.0007 0.018 0.0007 0.0043 0.0003 0.0076 0.0002 0.013 0.0044 0.0015 0.0003 0.0015 0
Cell Periphery 0.0006 0.0013 0.0006 0.0004 0.0002 0.0002 0.0007 0.0001 0.0003 0.0002 0.0005 0 0.0005 0.0001 0.0002 0.0001 0.0001 0
Cytoplasm 0.4955 0.6324 0.6279 0.7237 0.4432 0.6418 0.5172 0.8378 0.7346 0.8167 0.7087 0.8563 0.5923 0.799 0.854 0.802 0.7428 0.8814
Cytoplasmic Foci 0.0235 0.0349 0.0186 0.014 0.0149 0.0168 0.0502 0.0073 0.0394 0.0244 0.0167 0.0207 0.0398 0.0115 0.0096 0.0229 0.0283 0.0102
Eisosomes 0.0004 0.0003 0.0003 0.0001 0.0003 0.0001 0.0004 0 0.0002 0.0001 0.0001 0 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0097 0.0033 0.006 0.0128 0.0046 0.0037 0.0077 0.0014 0.0039 0.0037 0.0061 0.0019 0.0077 0.0021 0.0037 0.0032 0.0013 0.0022
Endosome 0.0219 0.0115 0.0135 0.0195 0.0168 0.0108 0.0585 0.0072 0.0278 0.0345 0.0516 0.0175 0.0357 0.0045 0.0071 0.0259 0.0287 0.009
Golgi 0.0035 0.0017 0.0017 0.0016 0.0064 0.0038 0.0255 0.0003 0.0113 0.0116 0.004 0.0086 0.0151 0.0008 0.0015 0.0104 0.034 0.0003
Lipid Particles 0.0059 0.0014 0.0028 0.0007 0.0023 0.0043 0.0227 0.0001 0.0093 0.0027 0.0023 0.001 0.0219 0.0002 0.001 0.0066 0.027 0
Mitochondria 0.0022 0.0005 0.0009 0.0012 0.0022 0.0015 0.0118 0.0001 0.0105 0.0206 0.0084 0.0061 0.006 0.0002 0.0013 0.0007 0.0272 0.0001
None 0.3703 0.2834 0.2832 0.1846 0.4192 0.3047 0.1818 0.1406 0.1018 0.0706 0.1222 0.0777 0.1872 0.1702 0.0853 0.1073 0.0126 0.094
Nuclear Periphery 0.0127 0.0005 0.0024 0.0024 0.0033 0.0006 0.0088 0.0004 0.0053 0.0009 0.0067 0.0006 0.0053 0.0004 0.0007 0.0009 0.0003 0.0003
Nucleolus 0.0007 0.0004 0.0004 0.0002 0.0002 0.0001 0.0015 0 0.0006 0.0001 0.0006 0 0.0055 0 0.0001 0 0.0001 0
Nucleus 0.0103 0.0019 0.0017 0.0018 0.0016 0.0008 0.0033 0.0006 0.0022 0.0008 0.0023 0.0006 0.0044 0.0005 0.0009 0.0009 0.0004 0.0004
Peroxisomes 0.0033 0.0007 0.0006 0.0002 0.0014 0.0017 0.016 0 0.0117 0.0005 0.0007 0.0043 0.0212 0.0003 0.0021 0.0035 0.0559 0
Punctate Nuclear 0.0043 0.0009 0.0035 0.0007 0.0015 0.001 0.0082 0.0002 0.0019 0.0004 0.001 0.0002 0.0058 0.0004 0.0003 0.0004 0.0003 0.0001
Vacuole 0.0056 0.0071 0.0058 0.0098 0.0035 0.0015 0.0069 0.0026 0.0074 0.0061 0.0109 0.0014 0.0041 0.0013 0.0044 0.0093 0.0022 0.001
Vacuole Periphery 0.0019 0.0004 0.0006 0.0016 0.0008 0.0003 0.0028 0.0003 0.0012 0.0014 0.004 0.0005 0.0011 0.0001 0.0004 0.0011 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.0437 19.2617 16.9521 20.7761 12.0192 16.0261 20.4945 23.6909 16.9939 24.0831
Translational Efficiency 0.7845 0.6918 0.7102 0.4577 0.828 0.7593 0.6718 0.3978 0.524 0.4399

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1365 1267 1673 1305 1735 1046 1606 2222 3100 2313 3279 3527

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 655.75 730.38 831.12 804.24 667.20 763.27 781.08 965.55 662.16 745.25 806.61 905.86
Standard Deviation 80.70 104.67 107.67 121.31 81.44 100.59 105.35 139.93 81.31 104.14 109.44 154.42
Intensity Change Log 2 — 0.155501 0.341911 0.294480 — 0.194074 0.227351 0.533232 — 0.175084 0.285272 0.419820

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000075 0.000169 0.000208 0.000483 0.000150 0.000613 0.000097 0.000070 0.000117 0.000370 0.000153 0.000222
Bud Neck 0.005128 0.006938 0.002252 0.006398 0.011212 0.023214 0.004412 0.007194 0.008533 0.014299 0.003310 0.006900
Bud Site 0.002719 0.013432 0.008642 0.028703 0.009835 0.031948 0.006644 0.015104 0.006702 0.021805 0.007663 0.020135
Cell Periphery 0.000164 0.000090 0.000072 0.000161 0.000117 0.000111 0.000669 0.000047 0.000138 0.000100 0.000364 0.000089
Cytoplasm 0.641848* 0.593631* 0.707856* 0.556628* 0.533530* 0.467282* 0.613772* 0.816657* 0.581225* 0.536493* 0.661775* 0.720445*
Cytoplasmic Foci 0.170457 0.195717* 0.017733 0.035022 0.251889* 0.276861* 0.042401 0.005626 0.216033* 0.232412* 0.029815 0.016503
Eisosomes 0.000126 0.000051 0.000020 0.000075 0.000117 0.000115 0.000738 0.000006 0.000121 0.000080 0.000372 0.000032
Endoplasmic Reticulum 0.000517 0.000875 0.001202 0.000815 0.001343 0.000425 0.000402 0.000065 0.000979 0.000672 0.000810 0.000343
Endosome 0.007269 0.029359 0.002147 0.008792 0.024121 0.054017 0.002823 0.001181 0.016701 0.040510 0.002478 0.003997
Golgi 0.001255 0.003710 0.000149 0.002309 0.005980 0.011528 0.000234 0.000697 0.003900 0.007245 0.000191 0.001293
Lipid Particles 0.003642 0.004187 0.000664 0.001804 0.006940 0.003194 0.001407 0.000144 0.005488 0.003738 0.001028 0.000758
Mitochondria 0.001901 0.001907 0.000363 0.004005 0.004856 0.008811 0.000199 0.001025 0.003555 0.005029 0.000283 0.002128
Mitotic Spindle 0.001108 0.002026 0.005966 0.037795 0.001013 0.002105 0.001926 0.013122 0.001055 0.002061 0.003987 0.022251
None 0.004966 0.003594 0.004397 0.003146 0.003032 0.000545 0.009794 0.006800 0.003884 0.002215 0.007040 0.005448
Nuclear Periphery 0.000244 0.000286 0.000635 0.000536 0.000222 0.000111 0.000325 0.000201 0.000232 0.000207 0.000483 0.000325
Nuclear Periphery Foci 0.000374 0.001564 0.001665 0.001025 0.000464 0.000308 0.000702 0.000056 0.000424 0.000996 0.001194 0.000414
Nucleolus 0.000772 0.001478 0.000190 0.000471 0.000717 0.000674 0.000859 0.000345 0.000741 0.001115 0.000518 0.000392
Nucleus 0.116663 0.040903 0.182746* 0.127239 0.042032 0.012350 0.203591* 0.091406 0.074894 0.027991 0.192956* 0.104664
Peroxisomes 0.001266 0.002913 0.000294 0.000893 0.002739 0.009357 0.000339 0.000284 0.002091 0.005827 0.000316 0.000509
Vacuole 0.038250 0.094208 0.062618 0.180225 0.096550 0.092626 0.108528 0.039690 0.070879 0.093493 0.085104 0.091689
Vacuole Periphery 0.001256 0.002963 0.000181 0.003474 0.003140 0.003804 0.000137 0.000281 0.002311 0.003343 0.000159 0.001462

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.13 -4.51 -4.24 -2.85 -1.04 -1.73 1.14 4.71 2.07 2.32 -2.08 -3.03 -1.91 1.62 0.86
Bud Neck -1.79 4.13 0.88 2.54 -2.93 -6.18 7.49 6.41 9.99 -1.74 -5.39 8.86 5.64 9.56 -3.54
Bud Site -9.09 -3.76 -7.28 -1.90 -4.52 -9.08 2.57 1.70 10.26 -1.12 -11.36 0.06 -4.20 6.91 -3.85
Cell Periphery 5.95 7.04 4.58 -1.41 -3.02 0.63 -3.24 13.90 9.08 3.97 5.19 -2.51 11.76 7.62 3.91
Cytoplasm 5.07 1.32 13.49 7.76 11.78 6.09 -8.24 -26.41 -27.61 -16.32 6.05 -5.92 -11.14 -15.51 -5.08
Cytoplasmic Foci -3.65 36.00 33.00 32.11 -5.84 -3.07 41.92 51.14 41.71 19.96 -2.93 54.30 59.98 52.44 13.17
Eisosomes 11.04 15.14 10.00 -1.05 -8.01 0.30 -1.55 20.80 15.44 1.94 7.79 -1.19 22.54 14.77 1.89
Endoplasmic Reticulum -2.52 -8.43 -2.61 0.17 7.40 5.52 5.81 8.45 3.77 10.37 2.73 -1.71 6.01 4.17 11.70
Endosome -11.54 7.88 3.07 12.95 -3.49 -9.79 20.05 21.85 18.64 7.78 -13.34 21.01 20.23 23.04 0.82
Golgi -4.57 2.87 0.53 5.58 -2.62 -5.19 16.39 15.12 11.02 -0.64 -5.85 14.12 11.01 12.72 -2.48
Lipid Particles -0.95 10.98 5.19 4.70 -1.06 6.44 14.00 16.54 6.92 11.00 4.36 17.37 16.78 8.74 2.64
Mitochondria 0.02 2.05 0.13 0.14 -2.03 -2.25 5.83 5.20 5.25 -6.21 -1.58 5.87 4.02 5.09 -3.06
Mitotic Spindle -0.70 -3.19 -6.87 -6.40 -5.24 -1.61 -2.19 -6.66 -5.39 -4.42 -1.36 -3.96 -9.40 -8.18 -6.56
None 1.53 1.84 3.26 0.80 2.77 4.28 -3.47 -4.31 -16.32 1.51 2.90 -2.48 -1.74 -5.32 1.57
Nuclear Periphery -0.66 -12.74 -8.73 -8.95 2.85 2.65 -7.15 -9.98 -9.88 -5.68 0.69 -15.27 -12.59 -12.48 0.65
Nuclear Periphery Foci -3.07 -10.61 -6.49 1.29 5.99 2.22 -4.10 7.00 4.48 10.27 -2.64 -11.17 -0.35 2.58 11.45
Nucleolus -2.24 5.01 1.73 3.18 -1.38 0.18 0.08 4.48 1.55 0.80 -1.95 1.65 3.73 3.78 0.15
Nucleus 16.99 -15.28 -0.06 -14.78 14.09 16.10 -25.52 -25.73 -39.08 9.31 18.88 -29.33 -15.35 -32.94 17.04
Peroxisomes -4.15 4.56 3.28 7.01 -2.32 -5.92 9.96 10.45 8.42 2.71 -6.72 10.72 10.54 10.40 -0.24
Vacuole -15.06 -16.52 -31.27 -22.11 -19.93 0.80 -8.46 0.04 -0.74 8.35 -7.80 -15.35 -22.56 -15.73 -8.04
Vacuole Periphery -3.19 2.42 -0.35 3.20 -3.46 -1.64 13.17 12.96 11.12 -0.68 -3.21 9.10 6.07 10.80 -3.47
Standard name
Human Ortholog
Description Non-catalytic subunit of the NatC N-terminal acetyltransferase; required for replication of dsRNA virus; expression is glucose-repressible; human NatC ortholog, Naa35, requires co-expression of the human catalytic subunit, Naa30, to functionally complement the null allele
Localization
Cell Percentages cytoplasm (77%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mak10

Mak10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mak10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available