Standard name
Human Ortholog
Description Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.98 0.97 0.95 0.86 0.85 0.85 0.87 0.84 0.83 1.0 0.99 0.98 0.98 0.98 0.99 0.98 0.93 0.93 0.96 0.93 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.07 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0 0.05 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 2 7 3 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 2 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 1 0 4 2 3 8 3 1 2 0 0 0 2 3 0 0 0 0 0 0 0
Cytoplasm 137 148 133 92 111 200 180 229 160 152 108 151 167 209 234 224 185 118 131 137 123 145 109 117
Endoplasmic Reticulum 0 1 1 0 1 6 15 19 8 9 6 8 0 2 1 9 6 4 0 0 0 0 1 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 1
Mitochondria 0 1 1 1 0 2 3 10 12 5 7 18 0 1 0 1 0 0 0 1 0 0 0 0
Nucleus 0 0 0 0 0 0 1 2 0 0 1 0 0 0 0 0 1 0 0 3 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 2 2 1 11 10 3 8 7 5 0 4 3 5 3 2 0 0 1 0 0 1
Unique Cell Count 138 150 134 94 114 211 209 271 188 175 128 182 167 211 238 228 188 119 135 148 133 151 118 135
Labelled Cell Count 139 151 136 97 114 214 213 276 199 180 131 187 167 216 238 242 199 124 135 148 133 151 118 135


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.7 24.7 20.6 19.1 21.0 24.1 21.7 21.5 21.2 20.0 20.0 20.0 31.7 33.3 34.3 40.1 43.6 42.6 19.9 21.1 24.3
Std Deviation (1e-4) 5.6 6.2 5.0 5.5 5.7 5.9 6.4 5.7 6.0 5.4 6.6 5.6 10.1 13.0 13.7 10.7 12.7 12.5 4.9 6.9 8.5
Intensity Change (Log2) – – – -0.11 0.03 0.23 0.07 0.06 0.04 -0.04 -0.04 -0.04 0.62 0.69 0.73 0.96 1.08 1.05 -0.05 0.04 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -1.2 -2.2 -4.2 -4.5 -4.4 -4.0 -4.4 -4.7 1.1 -0.2 -0.8 -0.8 -0.7 -0.1
Endoplasmic Reticulum 0 0 0 2.8 2.7 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.5 0 0 3.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.2998 24.7307 22.5194 20.1611 15.8711 22.3151 15.6426 20.3944 19.1692 18.5995 13.1302 18.2966 17.0779 23.9413 22.1467 17.8106 19.1523 21.4859
Actin 0.0431 0.0002 0.0142 0.0174 0.0194 0.0281 0.0353 0.0003 0.0158 0.0001 0.0256 0.0003 0.0581 0.0003 0.0115 0.0315 0.0001 0.0006
Bud 0.0007 0.0002 0.0007 0.0101 0.002 0.0003 0.0004 0.0001 0.0002 0 0.0012 0.0001 0.0008 0.0002 0.0015 0.0003 0 0.0068
Bud Neck 0.0008 0.0001 0.0065 0.0009 0.0012 0.0114 0.0012 0.0001 0.0009 0.0003 0.0009 0.0008 0.0072 0.0001 0.0007 0.0006 0.0002 0.0007
Bud Periphery 0.0013 0 0.0007 0.015 0.0026 0.0003 0.0003 0 0.0002 0 0.001 0 0.0007 0 0.0007 0.0002 0 0.0023
Bud Site 0.0025 0.0002 0.0033 0.0251 0.0042 0.0006 0.0033 0 0.001 0 0.0019 0 0.0049 0.0001 0.004 0.001 0 0.0004
Cell Periphery 0.0016 0 0.0002 0.0005 0.0002 0.0001 0.0003 0 0 0 0.0002 0 0.0002 0 0.0001 0.0001 0 0
Cytoplasm 0.8043 0.9923 0.8951 0.8284 0.7473 0.8979 0.8706 0.9909 0.9398 0.983 0.7467 0.9723 0.7417 0.9911 0.9436 0.8513 0.9825 0.9553
Cytoplasmic Foci 0.0105 0.0005 0.0068 0.0104 0.0264 0.0051 0.0111 0.0005 0.0106 0.0002 0.0283 0.0016 0.0297 0.0008 0.0091 0.0096 0.0008 0.001
Eisosomes 0.0002 0 0.0004 0.0003 0.0001 0.0001 0.0002 0 0.0001 0 0.0003 0 0.0003 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.009 0.0003 0.0059 0.0024 0.0052 0.0026 0.0097 0.0003 0.0019 0.0003 0.0153 0.0012 0.0031 0.0002 0.0016 0.0033 0.002 0.0011
Endosome 0.026 0.0001 0.0186 0.0135 0.0268 0.0037 0.0228 0.0002 0.0039 0.0001 0.0451 0.0008 0.0143 0.0001 0.0029 0.0162 0.0006 0.0004
Golgi 0.002 0 0.004 0.0016 0.021 0.0049 0.0025 0 0.0032 0 0.0084 0 0.0054 0 0.0009 0.0192 0 0
Lipid Particles 0.0022 0 0.0008 0.0075 0.0172 0.0022 0.0022 0 0.0035 0 0.023 0 0.0118 0 0.0007 0.0091 0 0
Mitochondria 0.0218 0.0001 0.0072 0.0091 0.0509 0.001 0.0008 0.0001 0.0003 0.0001 0.0265 0.0001 0.0021 0.0001 0.0021 0.0075 0 0.0016
None 0.029 0.0005 0.0008 0.0113 0.0008 0.0003 0.0038 0.0004 0.002 0.0003 0.0034 0.0006 0.0069 0.0006 0.002 0.0016 0.0004 0.0006
Nuclear Periphery 0.0058 0.0006 0.0059 0.01 0.0197 0.007 0.0085 0.0007 0.0015 0.0014 0.0182 0.0018 0.0095 0.0007 0.003 0.0206 0.0038 0.0028
Nucleolus 0.0008 0 0.0008 0.0015 0.0064 0.0001 0.0003 0 0.0001 0 0.0005 0 0.0008 0 0.0003 0.0002 0 0.0001
Nucleus 0.0188 0.0046 0.0183 0.0262 0.0164 0.0277 0.0161 0.0062 0.0084 0.0139 0.0097 0.0189 0.0789 0.0053 0.0106 0.0106 0.0088 0.0249
Peroxisomes 0.0008 0 0.0007 0.0019 0.0179 0.0044 0.0012 0 0.0036 0 0.0269 0 0.0136 0 0.0019 0.0084 0 0.0001
Punctate Nuclear 0.0031 0.0001 0.0018 0.0027 0.0019 0.0007 0.0045 0.0001 0.0028 0.0001 0.0063 0.0006 0.0078 0.0002 0.0016 0.0077 0.0002 0.0005
Vacuole 0.0122 0.0001 0.0051 0.0025 0.0074 0.0012 0.0042 0.0001 0.0002 0.0001 0.0047 0.0006 0.0017 0.0001 0.0006 0.0004 0.0004 0.0005
Vacuole Periphery 0.0035 0 0.0021 0.0019 0.0048 0.0005 0.0006 0 0.0001 0 0.0058 0.0001 0.0005 0 0.0003 0.0006 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 249.1055 188.4795 87.542 84.293 132.1266 220.0904 172.2201 123.1445 127.8149 144.4351
Translational Efficiency 1.2587 1.4018 1.4086 1.7757 1.4915 1.5311 1.5547 1.642 1.4649 1.5079

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
79 1306 1588 1617 1792 1317 254 111 1871 2623 1842 1728

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1934.26 1910.81 2018.43 2101.04 2172.33 1757.91 2218.05 2172.21 2162.28 1834.04 2045.96 2105.61
Standard Deviation 365.73 446.13 437.72 470.20 502.91 377.66 380.31 451.88 500.18 420.19 435.73 469.37
Intensity Change Log 2 — -0.017597 0.061452 0.119322 — -0.305382 0.030049 -0.000080 — -0.162664 0.044925 0.057393

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000780 0.000289 0.000184 0.000951 0.000211 0.000284 0.000647 0.000222 0.000235 0.000286 0.000248 0.000904
Bud Neck 0.001263 0.000521 0.000969 0.002328 0.000521 0.000560 0.004625 0.003179 0.000552 0.000540 0.001473 0.002383
Bud Site 0.006727 0.003190 0.001380 0.009026 0.001298 0.006344 0.005364 0.005799 0.001527 0.004773 0.001929 0.008819
Cell Periphery 0.000358 0.000104 0.000134 0.000126 0.000131 0.000111 0.000376 0.000616 0.000140 0.000108 0.000168 0.000158
Cytoplasm 0.759647* 0.799973* 0.613466* 0.735656* 0.721067* 0.829689* 0.513275* 0.735632* 0.722696* 0.814893* 0.599650* 0.735655*
Cytoplasmic Foci 0.022270 0.006630 0.003875 0.007751 0.003003 0.013085 0.010182 0.024105 0.003816 0.009871 0.004744 0.008801
Eisosomes 0.000004 0.000002 0.000001 0.000004 0.000001 0.000002 0.000012 0.000004 0.000001 0.000002 0.000003 0.000004
Endoplasmic Reticulum 0.000694 0.000311 0.000173 0.000245 0.000101 0.001387 0.001118 0.000075 0.000126 0.000851 0.000303 0.000234
Endosome 0.000500 0.000499 0.000252 0.003833 0.000076 0.001830 0.002543 0.000307 0.000094 0.001167 0.000568 0.003606
Golgi 0.002229 0.001645 0.000239 0.005795 0.000469 0.003261 0.000797 0.000200 0.000543 0.002456 0.000316 0.005436
Lipid Particles 0.000642 0.000321 0.000355 0.000836 0.000093 0.000526 0.002484 0.001282 0.000117 0.000424 0.000648 0.000864
Mitochondria 0.000252 0.000305 0.000286 0.000925 0.000249 0.001020 0.000264 0.000083 0.000249 0.000664 0.000283 0.000871
Mitotic Spindle 0.004037 0.005630 0.007372 0.034365 0.001135 0.006940 0.003921 0.003334 0.001257 0.006288 0.006896 0.032372
None 0.003733 0.012887 0.012124 0.009061 0.013594 0.011864 0.005598 0.015467 0.013177 0.012373 0.011224 0.009473
Nuclear Periphery 0.001853 0.000088 0.000394 0.000622 0.000073 0.000076 0.001036 0.000222 0.000148 0.000082 0.000482 0.000596
Nuclear Periphery Foci 0.016036 0.002262 0.001088 0.002643 0.000498 0.003324 0.006768 0.002546 0.001154 0.002795 0.001871 0.002637
Nucleolus 0.000692 0.000657 0.000124 0.000152 0.000083 0.000127 0.000260 0.000154 0.000109 0.000391 0.000142 0.000152
Nucleus 0.162946* 0.158835* 0.349536* 0.161255* 0.255135* 0.111702 0.273455* 0.161165* 0.251243* 0.135170* 0.339045* 0.161250*
Peroxisomes 0.000047 0.000082 0.000018 0.000365 0.000077 0.000167 0.000107 0.000067 0.000075 0.000125 0.000030 0.000346
Vacuole 0.011993 0.004833 0.007947 0.017550 0.001729 0.006447 0.166870 0.045467 0.002163 0.005643 0.029861 0.019343
Vacuole Periphery 0.003297 0.000938 0.000083 0.006511 0.000458 0.001254 0.000300 0.000073 0.000578 0.001097 0.000113 0.006098

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.24 2.11 1.89 -0.44 -0.17 -1.01 -5.89 -0.86 0.04 5.53 -0.81 -1.99 -1.04 -0.59 0.35
Bud Neck 1.94 -2.28 -1.08 -6.99 2.28 -1.15 -9.52 -6.66 -6.43 4.06 -0.97 -13.82 -8.72 -8.46 4.51
Bud Site 1.18 1.35 0.47 -2.33 -4.26 -4.25 -8.38 -8.17 -3.49 -2.62 -4.08 -4.69 -6.42 -1.53 -3.96
Cell Periphery 3.94 2.17 3.33 -2.76 4.08 1.49 -8.20 -4.66 -5.09 1.91 2.19 -6.97 -2.02 -4.73 5.56
Cytoplasm -1.53 8.89 1.91 11.02 -21.01 -14.73 20.47 7.53 11.42 -3.83 -14.48 25.80 2.72 16.94 -22.43
Cytoplasmic Foci 2.89 2.95 2.58 -2.29 -2.94 -9.59 -8.92 -7.59 -4.58 -4.51 -8.91 -6.60 -9.13 -0.55 -3.59
Eisosomes 2.83 0.22 0.42 -3.90 0.38 -2.27 -12.94 -7.35 -6.67 3.85 -1.60 -7.16 -4.59 -4.74 3.13
Endoplasmic Reticulum 1.51 1.86 2.54 1.97 4.79 -1.70 -7.78 0.89 1.73 7.95 -1.89 -6.49 1.46 2.01 7.09
Endosome 0.39 0.96 -0.99 -1.17 -1.41 -3.75 -8.75 -4.01 2.20 4.36 -4.37 -9.48 -2.43 0.33 -0.95
Golgi 0.52 1.54 0.71 0.32 -2.23 -2.75 -2.36 3.45 3.04 6.38 -3.19 2.30 -1.61 1.45 -2.09
Lipid Particles 1.88 -1.17 -2.75 -5.95 -2.33 -3.52 -10.11 -4.81 -4.13 -0.09 -4.85 -13.32 -9.41 -6.30 -1.07
Mitochondria -0.27 -1.31 -0.31 0.01 1.27 -0.84 -2.80 7.94 1.10 9.07 -0.85 -5.52 -0.02 0.82 1.45
Mitotic Spindle -0.53 -1.51 -2.13 -2.69 -1.25 -3.39 -4.75 -2.71 -1.92 -1.80 -4.44 -6.80 -5.82 -2.92 -1.68
None -9.32 -11.16 -6.36 5.68 7.29 3.55 14.64 5.39 3.25 -1.51 2.57 5.86 9.92 7.09 5.84
Nuclear Periphery 1.22 0.69 1.00 -5.22 5.33 -1.17 -17.01 -7.14 -6.98 7.17 0.83 -10.56 -3.61 -6.23 7.26
Nuclear Periphery Foci 3.66 3.91 3.90 2.34 -0.19 -5.84 -8.53 -5.26 0.15 2.47 -5.26 -4.01 -2.66 3.47 1.44
Nucleolus 0.05 0.85 0.96 1.13 5.23 -1.81 -10.73 -2.15 0.59 8.00 -1.25 -4.46 -1.13 1.13 7.23
Nucleus -0.56 -12.03 -0.74 -0.54 30.88 23.75 -0.47 15.46 3.80 12.03 20.64 -14.61 15.33 -4.75 31.50
Peroxisomes -0.71 0.49 -1.58 -0.66 -1.84 -0.96 -0.72 -0.54 0.80 0.47 -0.58 0.32 -0.49 0.12 -1.64
Vacuole 2.33 -5.11 -12.03 -20.31 -8.65 -8.03 -16.82 -12.15 -11.75 -1.57 -9.33 -18.79 -23.49 -21.12 -3.55
Vacuole Periphery 1.04 1.40 0.77 -0.95 -2.56 -1.25 1.68 2.97 2.18 8.13 -1.19 2.88 -1.47 -0.65 -2.52
Standard name
Human Ortholog
Description Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Frd1

Frd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Frd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available