Standard name | |
---|---|
Human Ortholog | |
Description | Predicted cytoskeleton protein involved in intracellular signaling; based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 5 | 8 | 12 | 4 | 4 | 1 | 2 | 2 | – | 1 | 1 | 14 | 24 | 11 | 55 | 37 | 53 | 9 | 6 | 26 | 13 | 13 | 43 |
Bud | 2 | 0 | 15 | 11 | 16 | 19 | 23 | 23 | 28 | – | 28 | 14 | 1 | 6 | 18 | 7 | 2 | 10 | 0 | 0 | 1 | 2 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 7 | 6 | 6 | 7 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 3 | 11 | 17 | 31 | 20 | 22 | 14 | 10 | – | 16 | 22 | 30 | 55 | 44 | 21 | 33 | 29 | 2 | 2 | 9 | 2 | 4 | 16 |
Cytoplasm | 24 | 57 | 190 | 175 | 301 | 316 | 416 | 460 | 352 | – | 265 | 263 | 112 | 209 | 231 | 227 | 153 | 160 | 3 | 23 | 27 | 23 | 39 | 32 |
Endoplasmic Reticulum | 59 | 42 | 22 | 21 | 17 | 30 | 18 | 10 | 11 | – | 2 | 38 | 68 | 37 | 26 | 16 | 11 | 8 | 82 | 65 | 142 | 116 | 107 | 150 |
Endosome | 2 | 0 | 0 | 1 | 10 | 4 | 4 | 9 | 2 | – | 0 | 4 | 1 | 2 | 0 | 7 | 6 | 4 | 1 | 1 | 5 | 3 | 5 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 3 | 1 | 2 | 2 |
Mitochondria | 1 | 10 | 19 | 40 | 28 | 86 | 143 | 176 | 229 | – | 200 | 300 | 1 | 2 | 15 | 12 | 9 | 8 | 6 | 10 | 45 | 22 | 37 | 37 |
Nucleus | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | – | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 4 | 3 | – | 1 | 1 | 0 | 0 | 0 | 3 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 1 | 0 | 6 | 0 | 17 | 3 | 17 | 13 | 2 | – | 5 | 10 | 0 | 0 | 2 | 5 | 6 | 8 | 2 | 5 | 10 | 9 | 20 | 12 |
Unique Cell Count | 90 | 105 | 240 | 238 | 378 | 414 | 550 | 610 | 522 | 416 | 513 | 195 | 277 | 290 | 318 | 233 | 250 | 116 | 123 | 285 | 206 | 246 | 319 | |
Labelled Cell Count | 104 | 117 | 272 | 279 | 432 | 487 | 649 | 715 | 640 | 519 | 655 | 227 | 336 | 348 | 359 | 266 | 283 | 116 | 123 | 285 | 206 | 246 | 319 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 5.7 | 4.8 | 4.8 | 4.9 | 4.5 | 4.3 | 4.2 | 3.5 | – | 3.7 | 3.6 | 6.4 | 6.2 | 5.4 | 7.2 | 7.9 | 8.0 | 6.3 | 5.6 | 6.3 |
Std Deviation (1e-4) | 0.9 | 0.9 | 0.8 | 1.1 | 1.3 | 1.1 | 1.2 | 0.9 | 0.8 | – | 0.9 | 1.0 | 1.2 | 1.0 | 0.9 | 1.0 | 1.2 | 1.2 | 1.0 | 1.1 | 1.2 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.03 | -0.1 | -0.17 | -0.19 | -0.45 | – | -0.39 | -0.43 | 0.41 | 0.39 | 0.18 | 0.6 | 0.73 | 0.74 | 0.39 | 0.24 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.8 | 2.5 | 0.3 | 5.2 | 4.6 | 6.0 |
Bud | 0 | -1.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | -3.2 | -2.3 | 0 | -2.4 | -3.1 | -1.1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.8 | 5.2 | 4.0 | 1.0 | 3.6 | 2.8 |
Cytoplasm | -1.5 | 0.1 | -0.8 | -1.1 | -1.2 | -3.3 | – | -4.1 | -7.3 | -4.9 | -1.0 | 0.1 | -2.1 | -3.3 | -3.7 |
Endoplasmic Reticulum | 0 | -2.3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 6.6 | 1.5 | -0.1 | -1.9 | -1.9 | -2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -0.2 | 0 | 0 | 0 | 0 | – | 0 | 0 | -3.7 | -4.1 | -1.3 | -2.1 | -1.9 | -2.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.3 | 0 | 0.5 | -0.3 | 0 | – | 0 | -0.5 | 0 | 0 | 0 | 0 | 0.1 | 0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6825 | 2.8354 | 2.5108 | 2.6144 | 2.3991 | 2.9515 | 3.2594 | 3.5417 | 3.165 | 2.8452 | 2.8256 | 3.209 | 3.4865 | 3.3717 | 3.412 | 3.2405 | 3.4906 | 3.3857 |
Actin | 0.0133 | 0.0098 | 0.0246 | 0.0081 | 0.0044 | 0.0058 | 0.022 | 0.0036 | 0.0148 | 0.0183 | 0.0071 | 0.0038 | 0.015 | 0.0038 | 0.0184 | 0.0353 | 0.0159 | 0.0099 |
Bud | 0.0001 | 0.0003 | 0.0007 | 0.0002 | 0.0004 | 0.0002 | 0.0007 | 0.0004 | 0.0013 | 0.0015 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0003 | 0.0002 |
Bud Neck | 0.0027 | 0.0016 | 0.0033 | 0.0031 | 0.0075 | 0.0291 | 0.0027 | 0.0011 | 0.0058 | 0.0125 | 0.0108 | 0.012 | 0.0019 | 0.0009 | 0.0028 | 0.0093 | 0.004 | 0.0151 |
Bud Periphery | 0.0005 | 0.0005 | 0.0027 | 0.0005 | 0.0063 | 0.0008 | 0.0016 | 0.0008 | 0.0033 | 0.0059 | 0.0025 | 0.0006 | 0.0006 | 0.0004 | 0.0024 | 0.0019 | 0.001 | 0.0005 |
Bud Site | 0.0022 | 0.0254 | 0.0057 | 0.0026 | 0.001 | 0.0005 | 0.0042 | 0.0182 | 0.0094 | 0.0067 | 0.0019 | 0.0003 | 0.0022 | 0.0279 | 0.0077 | 0.0027 | 0.0015 | 0.0003 |
Cell Periphery | 0.0183 | 0.0117 | 0.0138 | 0.0096 | 0.0205 | 0.0078 | 0.0096 | 0.0118 | 0.0121 | 0.0069 | 0.0075 | 0.0026 | 0.0152 | 0.0079 | 0.0151 | 0.0115 | 0.005 | 0.0054 |
Cytoplasm | 0.347 | 0.3714 | 0.4176 | 0.4145 | 0.4504 | 0.4874 | 0.3341 | 0.4445 | 0.3976 | 0.3263 | 0.3856 | 0.4378 | 0.3844 | 0.3955 | 0.4303 | 0.3924 | 0.4118 | 0.5489 |
Cytoplasmic Foci | 0.0251 | 0.027 | 0.028 | 0.0409 | 0.0363 | 0.0194 | 0.0385 | 0.029 | 0.0337 | 0.0916 | 0.0318 | 0.0152 | 0.017 | 0.0262 | 0.0276 | 0.0187 | 0.0324 | 0.0145 |
Eisosomes | 0.0015 | 0.0004 | 0.0006 | 0.0004 | 0.0004 | 0.0004 | 0.0008 | 0.0004 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0017 | 0.0006 | 0.0012 | 0.0017 | 0.0011 | 0.0007 |
Endoplasmic Reticulum | 0.3952 | 0.408 | 0.3528 | 0.2974 | 0.3709 | 0.3254 | 0.296 | 0.2973 | 0.302 | 0.2457 | 0.3269 | 0.3876 | 0.3829 | 0.2997 | 0.2831 | 0.3087 | 0.2214 | 0.2655 |
Endosome | 0.0281 | 0.0309 | 0.0312 | 0.0397 | 0.026 | 0.0239 | 0.0553 | 0.0289 | 0.0437 | 0.049 | 0.0511 | 0.0198 | 0.0153 | 0.0539 | 0.0264 | 0.0232 | 0.0246 | 0.0087 |
Golgi | 0.0092 | 0.0134 | 0.0098 | 0.0081 | 0.0037 | 0.0057 | 0.0153 | 0.0063 | 0.0144 | 0.0201 | 0.0137 | 0.0046 | 0.0061 | 0.0122 | 0.0099 | 0.0148 | 0.0121 | 0.0022 |
Lipid Particles | 0.0597 | 0.0435 | 0.044 | 0.0733 | 0.0345 | 0.0204 | 0.0659 | 0.0381 | 0.0361 | 0.0534 | 0.0385 | 0.0185 | 0.0492 | 0.0461 | 0.0436 | 0.043 | 0.0291 | 0.0136 |
Mitochondria | 0.0024 | 0.0012 | 0.0012 | 0.0007 | 0.0009 | 0.0014 | 0.0108 | 0.0023 | 0.0035 | 0.0115 | 0.0272 | 0.0018 | 0.0009 | 0.0022 | 0.0019 | 0.0083 | 0.002 | 0.0004 |
None | 0.0675 | 0.0296 | 0.0435 | 0.0861 | 0.0205 | 0.0585 | 0.0813 | 0.0918 | 0.091 | 0.0847 | 0.0321 | 0.0828 | 0.085 | 0.1063 | 0.1115 | 0.1101 | 0.2202 | 0.1064 |
Nuclear Periphery | 0.0112 | 0.0087 | 0.0049 | 0.0039 | 0.0056 | 0.0041 | 0.0154 | 0.0047 | 0.0075 | 0.0084 | 0.0115 | 0.0049 | 0.008 | 0.0062 | 0.0044 | 0.004 | 0.0051 | 0.0024 |
Nucleolus | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0014 | 0.0004 | 0.0006 | 0.0012 | 0.0043 | 0.0001 | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.001 | 0.0001 |
Nucleus | 0.003 | 0.0024 | 0.0019 | 0.0013 | 0.0021 | 0.0019 | 0.0066 | 0.0034 | 0.003 | 0.0049 | 0.011 | 0.0015 | 0.004 | 0.0013 | 0.0015 | 0.0013 | 0.0031 | 0.001 |
Peroxisomes | 0.0036 | 0.0007 | 0.0023 | 0.0008 | 0.0005 | 0.0004 | 0.0168 | 0.0045 | 0.0048 | 0.0278 | 0.009 | 0.0003 | 0.0008 | 0.0007 | 0.0023 | 0.0008 | 0.001 | 0.0003 |
Punctate Nuclear | 0.0019 | 0.0017 | 0.0015 | 0.0015 | 0.0012 | 0.0015 | 0.0093 | 0.0033 | 0.0051 | 0.0073 | 0.0127 | 0.0008 | 0.0014 | 0.0017 | 0.002 | 0.0016 | 0.0026 | 0.0007 |
Vacuole | 0.0064 | 0.0109 | 0.0092 | 0.0069 | 0.0062 | 0.0049 | 0.0097 | 0.0087 | 0.0092 | 0.014 | 0.0101 | 0.0043 | 0.0072 | 0.0055 | 0.0065 | 0.0093 | 0.0046 | 0.0029 |
Vacuole Periphery | 0.0007 | 0.0006 | 0.0005 | 0.0004 | 0.0004 | 0.0005 | 0.0022 | 0.0006 | 0.0008 | 0.0017 | 0.0037 | 0.0005 | 0.0004 | 0.0006 | 0.0005 | 0.0006 | 0.0005 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.6361 | 19.9268 | 26.0039 | 28.0198 | 19.4336 | 26.5039 | 31.3509 | 32.3551 | 26.3739 | 34.6131 |
Translational Efficiency | 0.4684 | 0.5904 | 0.4468 | 0.3166 | 0.4281 | 0.4759 | 0.3881 | 0.3962 | 0.4917 | 0.3803 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
624 | 672 | 181 | 1050 | 1398 | 1956 | 1336 | 145 | 2022 | 2628 | 1517 | 1195 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 694.74 | 784.43 | 1044.98 | 903.14 | 692.53 | 811.85 | 964.37 | 976.16 | 693.21 | 804.84 | 973.99 | 912.00 |
Standard Deviation | 108.06 | 104.66 | 115.34 | 107.79 | 92.14 | 99.80 | 105.65 | 121.17 | 97.34 | 101.77 | 110.00 | 112.07 |
Intensity Change Log 2 | — | 0.175172 | 0.588930 | 0.378476 | — | 0.229337 | 0.477710 | 0.495241 | — | 0.202465 | 0.534480 | 0.437947 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001152 | 0.005822 | 0.003652 | 0.014993 | 0.001977 | 0.002389 | 0.004944 | 0.055867 | 0.001722 | 0.003267 | 0.004790 | 0.019952 |
Bud Neck | 0.015416 | 0.022387 | 0.004665 | 0.022900 | 0.009417 | 0.013904 | 0.013858 | 0.023604 | 0.011268 | 0.016073 | 0.012762 | 0.022986 |
Bud Site | 0.006551 | 0.021781 | 0.013799 | 0.053611 | 0.006613 | 0.014586 | 0.024527 | 0.081735 | 0.006594 | 0.016426 | 0.023247 | 0.057023 |
Cell Periphery | 0.004542 | 0.003370 | 0.002068 | 0.002484 | 0.003176 | 0.002985 | 0.003196 | 0.012634 | 0.003597 | 0.003084 | 0.003062 | 0.003716 |
Cytoplasm | 0.262938* | 0.234326* | 0.168392* | 0.308788* | 0.289420* | 0.338810* | 0.327319* | 0.250911* | 0.281247* | 0.312092* | 0.308357* | 0.301765* |
Cytoplasmic Foci | 0.108416 | 0.194439* | 0.118200 | 0.139458 | 0.149506 | 0.127871 | 0.089007 | 0.092600 | 0.136825 | 0.144893 | 0.092491 | 0.133772 |
Eisosomes | 0.005939 | 0.005231 | 0.000679 | 0.004726 | 0.005584 | 0.005251 | 0.002299 | 0.011852 | 0.005693 | 0.005246 | 0.002106 | 0.005590 |
Endoplasmic Reticulum | 0.240502* | 0.174529* | 0.199831* | 0.049183 | 0.197337* | 0.191157* | 0.227140* | 0.132523* | 0.210658* | 0.186905* | 0.223881* | 0.059295 |
Endosome | 0.026337 | 0.044813 | 0.051194 | 0.024658 | 0.018817 | 0.024698 | 0.016580 | 0.012979 | 0.021138 | 0.029841 | 0.020710 | 0.023240 |
Golgi | 0.061093 | 0.077380 | 0.004372 | 0.013250 | 0.083525 | 0.065533 | 0.008598 | 0.010724 | 0.076603 | 0.068562 | 0.008094 | 0.012943 |
Lipid Particles | 0.162996* | 0.094958 | 0.081716 | 0.056438 | 0.123449* | 0.104327* | 0.069757 | 0.053042 | 0.135653* | 0.101931* | 0.071184 | 0.056026 |
Mitochondria | 0.025229 | 0.024845 | 0.000146 | 0.002838 | 0.036274 | 0.022955 | 0.000929 | 0.000301 | 0.032865 | 0.023438 | 0.000836 | 0.002530 |
Mitotic Spindle | 0.000103 | 0.001583 | 0.000554 | 0.007276 | 0.000566 | 0.001942 | 0.000673 | 0.000762 | 0.000423 | 0.001850 | 0.000659 | 0.006486 |
None | 0.004131 | 0.003289 | 0.000113 | 0.000623 | 0.006631 | 0.007429 | 0.000696 | 0.000211 | 0.005860 | 0.006370 | 0.000626 | 0.000573 |
Nuclear Periphery | 0.002212 | 0.001186 | 0.004752 | 0.000867 | 0.001162 | 0.000532 | 0.002895 | 0.000366 | 0.001486 | 0.000699 | 0.003117 | 0.000807 |
Nuclear Periphery Foci | 0.002902 | 0.002719 | 0.092756 | 0.009442 | 0.001831 | 0.001906 | 0.031810 | 0.016472 | 0.002162 | 0.002114 | 0.039082 | 0.010295 |
Nucleolus | 0.000553 | 0.000746 | 0.000262 | 0.000513 | 0.000875 | 0.000641 | 0.000249 | 0.000138 | 0.000775 | 0.000668 | 0.000251 | 0.000467 |
Nucleus | 0.016380 | 0.018095 | 0.029480 | 0.054482 | 0.021290 | 0.026497 | 0.085380 | 0.042541 | 0.019775 | 0.024348 | 0.078710 | 0.053033 |
Peroxisomes | 0.020430 | 0.019078 | 0.002203 | 0.011707 | 0.021254 | 0.016499 | 0.004335 | 0.007704 | 0.021000 | 0.017158 | 0.004081 | 0.011222 |
Vacuole | 0.025097 | 0.040900 | 0.220529* | 0.219909* | 0.016107 | 0.025520 | 0.085347 | 0.192545 | 0.018881 | 0.029453 | 0.101477 | 0.216589* |
Vacuole Periphery | 0.007080 | 0.008524 | 0.000636 | 0.001854 | 0.005190 | 0.004571 | 0.000457 | 0.000491 | 0.005773 | 0.005582 | 0.000479 | 0.001689 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.63 | -2.45 | -7.53 | -4.16 | -5.22 | -1.04 | -4.83 | -4.81 | -4.77 | -4.52 | -3.97 | -5.67 | -8.49 | -7.62 | -6.75 |
Bud Neck | -1.93 | 4.36 | -2.02 | 0.31 | -8.33 | -3.19 | -2.34 | -3.06 | -1.98 | -1.96 | -3.38 | -0.82 | -5.21 | -2.78 | -4.17 |
Bud Site | -5.60 | -2.09 | -10.43 | -6.24 | -6.96 | -5.99 | -7.29 | -6.32 | -5.58 | -4.66 | -8.06 | -7.55 | -12.28 | -9.51 | -7.17 |
Cell Periphery | 1.95 | 4.54 | 4.35 | 2.44 | -0.69 | 0.51 | 0.07 | -3.26 | -3.35 | -3.28 | 1.63 | 1.65 | 0.43 | -0.78 | -0.88 |
Cytoplasm | 2.58 | 5.93 | -3.46 | -6.34 | -8.60 | -6.68 | -4.18 | 2.44 | 5.25 | 4.37 | -4.89 | -3.32 | -1.67 | 2.04 | 1.26 |
Cytoplasmic Foci | -10.29 | -1.02 | -4.10 | 7.16 | -1.94 | 4.25 | 11.91 | 6.04 | 3.89 | -0.24 | -1.78 | 10.19 | 1.08 | 2.63 | -7.53 |
Eisosomes | 0.71 | 6.25 | 1.60 | 1.09 | -6.03 | 0.56 | 6.49 | -2.20 | -2.37 | -3.69 | 0.87 | 8.08 | 0.85 | 0.25 | -4.90 |
Endoplasmic Reticulum | 4.39 | 2.25 | 16.13 | 12.14 | 8.38 | 0.71 | -2.49 | 4.31 | 4.06 | 5.63 | 3.15 | -0.94 | 22.19 | 21.33 | 20.43 |
Endosome | -5.63 | -5.69 | 1.22 | 6.90 | 6.58 | -4.19 | 1.59 | 3.02 | 5.82 | 1.98 | -6.36 | 0.32 | -0.53 | 4.46 | -0.76 |
Golgi | -3.19 | 16.74 | 13.69 | 16.20 | -4.88 | 5.40 | 27.43 | 19.70 | 16.95 | -0.45 | 2.93 | 31.38 | 26.29 | 26.16 | -2.74 |
Lipid Particles | 7.95 | 8.43 | 14.06 | 7.15 | 3.60 | 3.90 | 10.94 | 8.71 | 6.77 | 2.45 | 7.82 | 14.54 | 18.06 | 12.63 | 4.45 |
Mitochondria | 0.12 | 9.88 | 8.58 | 8.81 | -3.32 | 5.48 | 16.26 | 16.56 | 20.85 | 5.37 | 4.77 | 18.85 | 16.85 | 17.59 | -2.27 |
Mitotic Spindle | -2.00 | -2.11 | -4.36 | -2.93 | -3.87 | -1.86 | -0.36 | -0.77 | 1.20 | -0.57 | -2.53 | -1.06 | -4.17 | -2.96 | -3.98 |
None | 0.37 | 3.44 | 3.01 | 2.29 | -2.72 | -0.53 | 6.12 | 6.64 | 7.21 | 3.25 | -0.47 | 6.84 | 6.89 | 7.14 | 0.46 |
Nuclear Periphery | 1.31 | -2.04 | 1.91 | 0.73 | 3.91 | 3.04 | -5.49 | 3.67 | 2.02 | 10.07 | 2.86 | -4.58 | 2.44 | -0.96 | 8.82 |
Nuclear Periphery Foci | 0.24 | -11.00 | -7.52 | -8.28 | 10.13 | -0.33 | -17.26 | -6.51 | -6.49 | 5.67 | 0.16 | -19.64 | -10.93 | -11.21 | 14.27 |
Nucleolus | -1.56 | 3.83 | 0.48 | 1.61 | -1.49 | 1.29 | 2.97 | 3.44 | 7.92 | 2.89 | -0.85 | 3.50 | 1.91 | 1.95 | -1.52 |
Nucleus | -1.10 | -3.40 | -13.72 | -10.40 | -5.56 | -3.49 | -19.35 | -4.71 | -3.62 | 7.62 | -3.82 | -19.97 | -13.70 | -11.47 | 7.16 |
Peroxisomes | 0.60 | 12.18 | 5.09 | 4.64 | -7.94 | 3.68 | 16.10 | 9.57 | 6.90 | -2.67 | 3.51 | 19.38 | 8.12 | 5.46 | -6.62 |
Vacuole | -4.22 | -14.51 | -28.05 | -25.18 | -1.07 | -6.08 | -21.29 | -11.11 | -10.61 | -7.07 | -6.92 | -23.83 | -31.67 | -30.02 | -17.21 |
Vacuole Periphery | -1.26 | 8.43 | 5.62 | 6.74 | -1.78 | 1.02 | 10.14 | 9.22 | 9.08 | 0.02 | 0.37 | 13.20 | 6.53 | 6.42 | -2.03 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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