TheCellVision

ANP1

Standard name	ANP1 ORF YEL036C Aliases GEM3, MNN8
Human Ortholog
Description	Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol

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Scale images

ANP1

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0.05	0.1	0	0	0	0	0	0	–	0	0	0	0.19	0.06	0	0.17	0.12	0	0	0	0	0	0	0
Bud	0	0.05	0.06	0.07	0.06	0.06	0	0	–	0.07	0.09	0.09	0	0	0.07	0.06	0.06	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	0	0.12	0	0	0.09	0	0	0	–	0	0	0	0.13	0.11	0.32	0.21	0.22	0.12	0	0	0	0	0	0
Endoplasmic Reticulum	0	0	0	0	0	0	0	0	–	0	0	0	0.13	0	0	0.06	0	0	0	0	0	0	0	0
Endosome	0.29	0	0.45	0.16	0.28	0	0	0	–	0	0	0	0.29	0.32	0.34	0.15	0.33	0.35	0.35	0.3	0.36	0.28	0.27	0.28
Golgi	0.17	0	0	0	0	0	0	0	–	0	0	0	0.13	0.11	0.06	0.28	0.16	0.35	0.45	0.28	0.4	0.41	0.44	0.38
Mitochondria	0.57	0.74	0.54	0.75	0.64	0.84	0.84	0.84	–	0.91	0.91	0.87	0.13	0.28	0.08	0.08	0.12	0.12	0.08	0.23	0.08	0.12	0.14	0.14
Nucleus	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	0.1	–	0.05	0	0	0	0	0	0	0	0.06	0	0	0	0	0	0
Peroxisomes	0	0	0.15	0.08	0.1	0.09	0.13	0.1	–	0.07	0.07	0.09	0	0.07	0.08	0	0	0.12	0.05	0	0.1	0.1	0.05	0.05
SpindlePole	0.07	0	0.14	0.08	0.07	0.07	0.09	0.07	–	0.06	0.06	0.07	0.1	0.1	0.09	0.11	0.14	0.18	0	0	0	0	0	0
Vac/Vac Membrane	0.11	0.13	0	0	0	0	0	0	–	0	0	0	0.16	0.1	0.08	0.15	0.1	0.24	0	0.08	0	0	0.05	0.06

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	10	20	4	0	0	1	0	2	–	1	3	3	12	6	3	9	10	0	3	4	4	5	1	5
Bud	7	11	20	12	13	23	18	11	–	30	35	34	0	0	8	3	5	0	0	0	0	1	0	1
Bud Neck	0	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	1	1	2	10	4	2	–	5	6	5	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	4	0	0	2	0	3	0	–	0	3	2	1	1	0	1	1	0	0	0	0	0	0	0
Cytoplasm	4	24	15	7	20	15	11	11	–	6	2	6	8	12	35	11	18	2	1	4	0	0	1	1
Endoplasmic Reticulum	5	4	2	2	0	1	1	1	–	0	2	1	8	2	3	3	2	0	0	1	1	1	1	3
Endosome	62	9	161	28	65	17	11	9	–	9	7	9	18	34	37	8	27	6	92	75	148	44	56	63
Golgi	37	8	10	2	4	0	0	0	–	0	2	0	8	12	7	15	13	6	118	71	166	66	92	85
Mitochondria	124	153	192	131	147	328	337	297	–	367	352	326	8	29	9	4	10	2	20	57	33	19	28	31
Nucleus	2	1	1	0	1	1	1	3	–	2	0	2	1	0	0	0	1	0	0	1	0	0	0	0
Nuclear Periphery	0	0	0	0	0	2	2	1	–	6	1	2	0	1	0	0	3	0	0	1	0	0	0	0
Nucleolus	5	0	3	4	2	14	14	35	–	21	14	13	0	2	0	0	1	1	0	0	0	0	1	0
Peroxisomes	3	3	53	14	24	34	51	34	–	28	26	35	0	7	9	0	0	2	12	5	40	16	10	11
SpindlePole	16	2	50	14	17	26	35	23	–	24	25	25	6	10	10	6	11	3	2	2	4	0	1	0
Vac/Vac Membrane	24	27	14	1	5	6	1	2	–	1	2	3	10	11	9	8	8	4	9	20	6	3	10	13
Unique Cell Count	217	208	355	174	229	389	400	353		404	386	376	63	105	109	53	81	17	264	251	411	160	208	225
Labelled Cell Count	299	266	526	216	302	478	489	431		500	480	466	80	127	130	68	110	26	264	251	411	160	208	225

Yeast GFP Assignment

Golgi

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	6.1	5.9	5.4	5.1	5.7	4.9	4.9	5.1	–	4.9	5.3	5.2	7.1	6.5	6.7	7.8	7.6	9.8	7.2	7.3	7.9
Std Deviation (1e^-4)	0.8	1.2	1.1	1.0	1.8	1.1	1.3	1.0	–	1.1	1.0	1.1	1.1	1.1	1.4	1.7	1.6	2.6	1.6	1.6	2.3
Intensity Change (Log₂)	–	–	–	-0.07	0.08	-0.15	-0.13	-0.09	–	-0.13	-0.04	-0.05	0.39	0.27	0.31	0.52	0.5	0.86	0.41	0.43	0.55

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0
Bud	0.6	0	0	-0.7	-1.6	–	1.0	1.8	1.8	0	0	0.7	0	0	0
Bud Neck	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0
Cell Periphery	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0
Cytoplasm	-0.1	2.2	-0.3	-1.1	-0.8	–	-2.3	-3.4	-2.1	0	2.8	8.2	0	5.5	0
Endoplasmic Reticulum	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0
Endosome	-6.6	-4.1	0	-13.9	-13.3	–	-14.2	-14.1	-13.7	-2.5	-2.4	-2.1	-4.2	-2.0	-0.8
Golgi	0	-0.8	0	0	0	–	0	-2.5	0	0	3.6	0	0	0	0
Mitochondria	4.7	2.4	0	9.0	8.6	–	11.5	11.4	9.7	-6.1	-4.8	-8.4	-6.3	-6.8	-3.4
Nucleus	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	2.5	5.4	–	3.4	2.5	2.4	0	0	0	0	0	0
Peroxisomes	-2.2	-1.6	0	-0.9	-2.1	–	-3.6	-3.6	-2.3	-3.3	-2.2	-1.8	-3.0	-3.7	0
SpindlePole	-2.0	-2.5	0	-2.3	-3.3	–	-3.8	-3.4	-3.3	-1.0	-1.2	-1.3	-0.5	-0.1	0
Vacuole	0	-1.2	-2.0	-3.6	-3.0	–	-3.7	-3.2	-2.8	0	2.6	1.8	0	0	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	2.9718	4.4192	3.8104	3.8957	3.1394	4.1839	5.8215	7.339	7.023	5.4735	7.2754	7.1199	5.8773	7.3315	6.9827	7.1434	6.8568	7.1242
Actin	0.0062	0.0048	0.0092	0.0014	0.0251	0.0059	0.0173	0.0024	0.0222	0.0021	0.0024	0.012	0.0209	0.0047	0.0203	0.001	0.0004	0.0106
Bud	0.0003	0.0002	0.0002	0.0001	0.0024	0.0001	0.0002	0.0001	0.0002	0	0	0.0001	0.0001	0.0001	0.0001	0	0	0
Bud Neck	0.0019	0.0003	0.0002	0.0002	0.0021	0.0002	0.0011	0.0002	0.0011	0.0013	0.0001	0.0002	0.0002	0.0002	0.0002	0.0001	0.0001	0.0002
Bud Periphery	0.0005	0.0001	0.0002	0.0001	0.0035	0.0001	0.0002	0.0001	0.0002	0	0	0.0001	0.0001	0.0001	0.0001	0	0	0
Bud Site	0.0083	0.0005	0.0005	0.0002	0.0082	0.0001	0.004	0.0041	0.0047	0.0001	0.0001	0.0003	0.0002	0.0002	0.0002	0	0	0.0001
Cell Periphery	0.0004	0	0	0	0.0005	0	0.0001	0	0.0001	0	0	0	0	0	0	0	0	0
Cytoplasm	0.0016	0.0023	0.0034	0.001	0.0027	0.0009	0.0021	0.0013	0.0015	0.0005	0.0006	0.0007	0.0012	0.0006	0.0006	0.0005	0.0003	0.0005
Cytoplasmic Foci	0.1141	0.1463	0.0974	0.1035	0.0999	0.0885	0.1365	0.1494	0.1315	0.1353	0.1211	0.106	0.1155	0.1232	0.1072	0.1026	0.0894	0.0859
Eisosomes	0.0002	0	0.0001	0	0.0002	0	0.0001	0	0.0002	0	0	0	0.0001	0	0	0	0	0
Endoplasmic Reticulum	0.0006	0.0004	0.0012	0.0002	0.0012	0.0002	0.0008	0.0002	0.0009	0.0001	0.0002	0.0002	0.0008	0.0001	0.0001	0.0001	0.0001	0.0002
Endosome	0.2505	0.2721	0.2765	0.2648	0.2574	0.155	0.1874	0.1805	0.1611	0.1237	0.1752	0.1247	0.1804	0.157	0.1386	0.2057	0.1137	0.112
Golgi	0.4445	0.5147	0.5391	0.5729	0.4055	0.657	0.5547	0.6225	0.5863	0.6569	0.6271	0.6567	0.5985	0.6739	0.6668	0.6694	0.6818	0.7141
Lipid Particles	0.0507	0.0124	0.0106	0.0207	0.0342	0.0269	0.0157	0.0031	0.012	0.0036	0.0241	0.0202	0.0176	0.0083	0.0059	0.0031	0.0725	0.0285
Mitochondria	0.0513	0.0206	0.0256	0.0061	0.0455	0.0269	0.016	0.009	0.0146	0.0046	0.0199	0.0198	0.019	0.0072	0.0214	0.0112	0.0029	0.014
None	0.0005	0.0001	0.0007	0	0.0023	0	0.0015	0.0001	0.002	0	0	0.0001	0.0003	0	0	0	0	0
Nuclear Periphery	0.0003	0.0001	0.0021	0.0001	0.0044	0	0.0005	0	0.0007	0	0.0001	0.0001	0.0004	0	0	0	0	0
Nucleolus	0.0005	0	0.0001	0	0.0198	0	0.0001	0	0.0001	0	0	0.0008	0	0	0	0	0	0
Nucleus	0.0003	0	0.001	0	0.0481	0	0.0002	0	0.0002	0	0	0.0006	0.0001	0	0	0	0	0
Peroxisomes	0.0622	0.0227	0.0275	0.0264	0.0189	0.0366	0.0589	0.0261	0.0579	0.0711	0.0271	0.0554	0.0426	0.0212	0.0376	0.0053	0.0377	0.0325
Punctate Nuclear	0.0006	0.0002	0.001	0.0001	0.004	0.0001	0.0008	0.0001	0.0013	0.0001	0.0001	0.0005	0.0007	0.0001	0.0001	0	0.0001	0.0001
Vacuole	0.0022	0.001	0.0014	0.0009	0.0089	0.0004	0.001	0.0003	0.0006	0.0001	0.0006	0.0006	0.0005	0.0016	0.0002	0.0002	0.0003	0.0004
Vacuole Periphery	0.0022	0.0013	0.0021	0.0013	0.0053	0.0009	0.0009	0.0005	0.0008	0.0003	0.0011	0.0009	0.0009	0.0016	0.0005	0.0007	0.0007	0.0008

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	49.187	63.6583	39.4455	37.034	36.1698	53.5622	44.9559	41.4266	44.5345	42.6601
Translational Efficiency	0.7551	0.6807	0.7658	0.804	0.9286	0.5579	0.6831	0.7384	0.5729	0.5713

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✘
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
120	794	1471	817	1748	1039	2002	1426	1868	1833	3473	2243

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	833.55	760.16	998.69	926.23	757.00	823.39	1015.40	869.05	761.92	796.00	1008.32	889.88
Standard Deviation	112.23	113.97	132.69	169.84	100.99	102.64	127.70	247.29	103.47	112.16	130.10	223.92
Intensity Change Log ₂	—	-0.132966	0.260768	0.152102	—	0.121283	0.423683	0.199146	—	-0.006363	0.340602	0.174683

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.001371	0.002180	0.003124	0.011852	0.003899	0.001170	0.005958	0.002836	0.003736	0.001607	0.004758	0.006120
Bud Neck	0.003727	0.000646	0.000235	0.002212	0.001170	0.000581	0.000124	0.001861	0.001334	0.000609	0.000171	0.001989
Bud Site	0.018442	0.001070	0.002000	0.025070	0.001774	0.002120	0.001842	0.010649	0.002844	0.001665	0.001909	0.015902
Cell Periphery	0.000424	0.000040	0.000032	0.000093	0.000180	0.000063	0.000026	0.000115	0.000195	0.000053	0.000028	0.000107
Cytoplasm	0.034625	0.001718	0.001164	0.024410	0.001691	0.004341	0.001050	0.009648	0.003807	0.003205	0.001098	0.015025
Cytoplasmic Foci	0.275067*	0.275189*	0.329836*	0.259150*	0.181341*	0.220058*	0.308503*	0.228177*	0.187362*	0.243939*	0.317538*	0.239459*
Eisosomes	0.000059	0.000048	0.000028	0.000051	0.000094	0.000072	0.000034	0.000036	0.000092	0.000061	0.000031	0.000041
Endoplasmic Reticulum	0.000908	0.000356	0.000729	0.000466	0.000112	0.000152	0.000528	0.000519	0.000163	0.000240	0.000613	0.000500
Endosome	0.266231*	0.215250*	0.167543	0.278188*	0.187677	0.177696	0.177181	0.394946*	0.192723	0.193964	0.173099	0.352417*
Golgi	0.177946*	0.437460*	0.451093*	0.254607*	0.548365*	0.524899*	0.470888*	0.244888*	0.524569*	0.487023*	0.462504*	0.248428*
Lipid Particles	0.005761	0.018732	0.007766	0.005106	0.017714	0.007831	0.003061	0.015862	0.016946	0.012553	0.005054	0.011944
Mitochondria	0.008953	0.012182	0.001804	0.017004	0.031672	0.029757	0.003838	0.009464	0.030213	0.022144	0.002977	0.012211
Mitotic Spindle	0.018924	0.005580	0.015711	0.045083	0.001404	0.005835	0.011779	0.023003	0.002530	0.005724	0.013445	0.031046
None	0.000034	0.000266	0.000009	0.000680	0.000048	0.000288	0.000007	0.001057	0.000047	0.000279	0.000008	0.000920
Nuclear Periphery	0.001112	0.000027	0.000118	0.000484	0.000015	0.000036	0.000046	0.000173	0.000085	0.000032	0.000077	0.000286
Nuclear Periphery Foci	0.001913	0.000380	0.001281	0.001403	0.000092	0.000098	0.000424	0.001520	0.000209	0.000220	0.000787	0.001477
Nucleolus	0.000355	0.000849	0.000006	0.000148	0.000057	0.000051	0.000004	0.000417	0.000076	0.000396	0.000005	0.000319
Nucleus	0.001930	0.000284	0.000022	0.000434	0.000104	0.000167	0.000020	0.000863	0.000221	0.000218	0.000021	0.000707
Peroxisomes	0.054243	0.022744	0.002852	0.021823	0.018598	0.014966	0.004119	0.012369	0.020888	0.018335	0.003582	0.015813
Vacuole	0.122440	0.003256	0.010394	0.045224	0.001734	0.005080	0.007970	0.034060	0.009489	0.004290	0.008997	0.038126
Vacuole Periphery	0.005536	0.001744	0.004253	0.006513	0.002260	0.004737	0.002599	0.007538	0.002470	0.003441	0.003299	0.007165

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-0.99	-2.14	-4.59	-4.20	-3.79	3.39	-1.85	1.08	-2.49	2.94	2.72	-1.20	-2.03	-4.73	-1.12
Bud Neck	4.03	4.79	1.68	-4.83	-7.60	1.55	2.92	-1.60	-3.85	-5.48	2.01	3.42	-1.91	-5.68	-8.24
Bud Site	2.80	2.64	-1.18	-8.97	-8.59	-0.42	-0.30	-6.75	-6.05	-6.92	1.67	1.44	-9.54	-10.79	-10.94
Cell Periphery	1.09	1.12	0.93	-4.28	-5.41	1.72	2.32	0.85	-1.47	-2.71	2.14	2.53	1.25	-2.37	-3.69
Cytoplasm	4.06	4.12	0.82	-7.90	-8.05	-2.38	1.47	-6.14	-3.57	-6.72	0.84	3.99	-7.91	-8.48	-10.36
Cytoplasmic Foci	-0.07	-3.11	1.09	2.47	9.58	-6.93	-24.04	-8.28	-1.24	13.40	-11.38	-28.34	-9.95	1.29	16.75
Eisosomes	0.66	2.04	0.47	-0.48	-4.46	2.03	7.03	6.58	4.40	-0.60	3.34	7.53	6.10	3.80	-3.54
Endoplasmic Reticulum	1.17	0.39	0.96	-0.64	2.11	-1.09	-3.38	-2.09	-1.87	0.05	-0.92	-5.29	-2.65	-1.77	0.69
Endosome	2.29	4.77	-0.40	-5.53	-12.07	1.49	2.05	-25.39	-24.71	-28.41*	-0.26	4.12	-23.06	-22.84	-29.86*
Golgi	-11.77	-12.71	-3.13	16.66	19.89*	2.44	10.12	36.16*	28.70	28.67*	4.54	8.69	36.19	32.43	34.72*
Lipid Particles	-4.25	-0.96	0.03	5.04	1.50	4.73	8.55	0.53	-3.88	-7.17	2.12	7.18	2.25	0.10	-5.61
Mitochondria	-0.67	1.45	-1.38	-1.23	-5.01	0.47	10.56	7.89	6.05	-4.34	2.57	11.08	6.62	4.31	-6.73
Mitotic Spindle	1.43	0.30	-2.93	-7.98	-5.93	-3.55	-6.24	-8.39	-6.25	-3.87	-2.69	-7.61	-11.64	-10.03	-6.82
None	-1.29	1.98	-1.22	-0.73	-1.27	-1.12	2.90	-1.59	-1.14	-1.65	-1.60	2.95	-1.94	-1.35	-2.03
Nuclear Periphery	1.01	0.93	0.54	-1.60	-1.30	-2.16	-4.42	-4.83	-4.09	-3.85	0.76	0.10	-1.66	-2.41	-2.00
Nuclear Periphery Foci	0.92	0.36	0.20	-2.44	-0.52	-0.22	-3.00	-5.20	-5.18	-3.80	-0.13	-3.87	-5.15	-5.35	-2.97
Nucleolus	-0.85	1.40	0.76	1.22	-2.29	0.16	1.54	-2.78	-2.89	-3.22	-1.29	1.98	-2.77	0.22	-3.68
Nucleus	2.52	3.01	2.08	-1.38	-3.95	-0.66	1.39	-1.66	-1.54	-1.83	0.05	2.91	-1.80	-1.80	-2.46
Peroxisomes	2.65	4.34	2.71	0.19	-8.02	1.56	9.06	3.09	1.13	-6.65	1.17	10.37	2.42	1.25	-10.45
Vacuole	5.57	5.25	3.09	-11.20	-9.78	-4.60	-9.26	-13.32	-11.64	-10.58	3.23	0.43	-11.88	-16.13	-14.28
Vacuole Periphery	2.11	0.66	-0.49	-5.83	-2.48	-3.68	-1.02	-6.53	-2.84	-6.32	-2.11	-2.43	-7.46	-5.50	-6.46