Standard name
Human Ortholog
Description Protein of unknown function may be involved in glutathione metabolism; function suggested by computational analysis of large-scale protein-protein interaction data; N- and C-terminal fusion proteins localize to the cell periphery
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.12 0.11 0.12 0.14 0.17 0.13 0.2 0.11 0.18 0.12 0.11 0.32 0.28 0.13 0.12 0.08 0 0 0 0.06 0 0
Endoplasmic Reticulum 0.15 0.13 0.06 0 0 0 0 0 0 0 0 0 0.47 0.19 0.18 0 0 0 0.19 0.18 0.28 0.29 0.39 0.39
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Mitochondria 0.2 0 0.06 0.07 0.07 0.21 0.26 0.2 0.34 0.24 0.28 0.21 0 0 0 0 0.1 0 0 0 0.06 0.07 0.13 0.09
Nucleus 0.06 0.06 0.06 0.19 0.2 0.18 0.17 0.21 0.25 0.28 0.3 0.38 0 0.08 0.08 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.44 0.49 0.12 0.39 0.24 0.15 0.24 0.18 0.17 0.25 0.22 0.17 0.3 0.05 0.14 0.22 0.29 0.35 0.61 0.66 0.41 0.35 0.24 0.18
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.51 0.45 0.62 0.37 0.51 0.41 0.36 0.43 0.25 0.28 0.21 0.3 0.29 0.42 0.4 0.6 0.52 0.57 0.09 0.08 0.18 0.11 0.13 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 1 0 0 0 1 0 2 1 1 0 2 0 1 1 2 2
Bud 0 0 0 0 1 0 1 0 0 0 1 1 0 0 0 0 1 0 5 0 0 3 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 2 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 6 3 1 0 1 0 0 0 0 0 0
Cytoplasm 8 6 2 6 16 16 29 14 26 16 25 17 8 42 36 8 6 3 1 1 0 6 6 2
Endoplasmic Reticulum 29 9 1 1 1 2 4 2 2 5 3 0 34 25 23 0 2 0 47 19 25 34 84 90
Endosome 0 1 0 0 1 0 0 0 0 0 2 0 1 6 4 1 1 1 3 0 1 2 4 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 0 0 0 0 1 2
Mitochondria 39 1 1 4 10 25 43 22 45 36 39 29 1 2 1 0 5 0 8 1 5 8 29 21
Nucleus 12 4 1 10 28 21 29 23 33 42 43 54 2 11 10 1 2 1 1 0 0 1 0 2
Nuclear Periphery 86 33 2 21 34 18 41 19 23 37 31 24 22 7 18 14 15 13 148 72 37 42 52 42
Nucleolus 0 1 0 0 0 2 3 0 0 1 1 4 0 0 0 1 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 1
Vac/Vac Membrane 100 30 10 20 71 48 60 46 33 42 30 43 21 55 51 38 27 21 22 8 17 13 27 47
Unique Cell Count 195 67 16 54 139 118 168 108 133 148 141 141 73 132 128 63 52 37 245 109 93 120 218 231
Labelled Cell Count 274 85 17 62 163 132 211 126 163 181 178 174 91 154 148 68 60 42 245 109 93 120 218 231


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 8.6 5.7 6.1 6.9 6.0 6.3 6.7 6.6 6.1 6.9 6.7 6.3 7.5 7.5 12.0 11.5 12.1 6.3 7.0 7.6
Std Deviation (1e-4) 0.8 1.4 0.8 1.3 1.6 1.3 1.6 1.6 1.9 1.5 2.5 1.8 1.0 1.6 1.5 2.5 2.4 2.4 1.2 1.5 1.6
Intensity Change (Log2) – – – 0.11 0.27 0.09 0.15 0.24 0.21 0.1 0.29 0.23 0.15 0.4 0.4 1.08 1.01 1.09 0.14 0.3 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 0 0 – 0 – 0 3.0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Nuclear Periphery 2.0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 – 2.5 0 0 0 0 0 0
Vacuole -1.8 -0.9 -1.7 -2.1 -1.5 – -2.8 – -2.6 -2.6 -1.6 -1.7 -0.2 -0.7 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.2759 5.9019 5.2843 5.2008 5.3012 5.8216 5.8214 5.165 4.9147 4.6488 5.6787 5.207 6.8262 5.7271 5.5919 5.2792 6.7488 5.7615
Actin 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0027 0.0001 0.0026 0.0001 0 0.0001 0.0094 0.0008 0.0009 0.0327 0.0001 0.0001
Bud 0.0001 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0.0019 0.0001 0.0001 0 0
Bud Neck 0 0.0001 0 0.0004 0.0001 0.0002 0.0002 0.0002 0.0001 0 0 0.0001 0.0005 0.0001 0.0001 0.0003 0 0.0001
Bud Periphery 0 0 0 0.0002 0 0 0 0 0 0 0 0 0.0001 0.0008 0.0001 0.0001 0 0
Bud Site 0.0001 0 0.0001 0.0002 0 0 0.0001 0 0.0001 0 0 0 0.0001 0.001 0.0004 0.0003 0 0
Cell Periphery 0 0 0 0.0002 0 0 0 0.0001 0 0 0 0 0 0.0002 0.0001 0.0001 0 0
Cytoplasm 0.0021 0.0027 0.0008 0.0005 0.0013 0.0021 0.0006 0.0113 0.0045 0.0002 0.0001 0.0001 0.0013 0.005 0.0031 0.0019 0.0002 0.0001
Cytoplasmic Foci 0.001 0.0003 0.0001 0.0002 0.0001 0.0026 0.0017 0.0002 0.0003 0 0 0 0.0009 0.0004 0.001 0.0068 0 0
Eisosomes 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0123 0.0519 0.0369 0.0621 0.0369 0.0134 0.0178 0.0843 0.0466 0.0924 0.0142 0.0163 0.0097 0.0674 0.0446 0.1205 0.0405 0.0088
Endosome 0.0008 0.0011 0.0004 0.0011 0.0007 0.0004 0.0089 0.002 0.0025 0.0007 0.0003 0.0003 0.0046 0.0006 0.0021 0.0301 0.0009 0.0003
Golgi 0 0 0 0.0002 0 0 0.0015 0 0.0005 0 0 0 0.0006 0 0.0003 0.0073 0.0001 0
Lipid Particles 0.0003 0.0002 0 0.0023 0 0.0007 0.0016 0.0002 0.0006 0 0 0 0.0012 0.0002 0.0009 0.0219 0.0002 0.0001
Mitochondria 0.0004 0.0002 0.0004 0.0004 0.0001 0.0002 0.0006 0.0002 0.0008 0.0003 0.0001 0.0002 0.0005 0.0011 0.0031 0.0033 0.0004 0.0001
None 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0003 0.0002 0.0011 0 0 0 0.0008 0.0004 0.0006 0.0023 0 0
Nuclear Periphery 0.8971 0.7889 0.86 0.8141 0.8547 0.9143 0.9086 0.8258 0.8646 0.8582 0.961 0.9455 0.9167 0.8305 0.8748 0.7243 0.9086 0.9563
Nucleolus 0.0019 0.0021 0.0016 0.0259 0.0002 0.0009 0.0013 0.0002 0.0003 0 0 0.0001 0.0003 0.0013 0.0004 0.0002 0 0.0006
Nucleus 0.0553 0.0994 0.0634 0.0668 0.0639 0.0412 0.026 0.0378 0.0395 0.0196 0.0114 0.0225 0.0248 0.0523 0.0183 0.0114 0.0207 0.017
Peroxisomes 0 0 0 0.0001 0 0.0001 0.0004 0 0.0001 0 0 0 0.0004 0 0.0034 0.002 0 0
Punctate Nuclear 0.0064 0.0272 0.0045 0.0005 0.0294 0.0068 0.0033 0.0004 0.001 0 0.0001 0.0001 0.0019 0.0047 0.0041 0.0029 0.0002 0.0003
Vacuole 0.001 0.0031 0.0012 0.0037 0.0008 0.0016 0.0011 0.0111 0.0025 0.0012 0.0005 0.0007 0.001 0.0034 0.0017 0.0042 0.0023 0.0008
Vacuole Periphery 0.0206 0.0227 0.0301 0.0208 0.0116 0.0149 0.0233 0.0258 0.0322 0.0273 0.0122 0.014 0.0252 0.0278 0.0399 0.0272 0.0258 0.0156

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.4688 40.8558 27.4295 23.9029 33.1552 41.3276 42.7846 35.5151 35.5178 31.009
Translational Efficiency 1.2665 1.3027 1.0411 1.2864 1.2256 0.9136 1.0423 1.392 1.0892 1.2104

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1811 1019 1496 143 2397 1862 374 508 4208 2881 1870 651

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 877.72 852.59 1062.49 973.85 812.16 913.16 1122.01 1017.43 840.38 891.74 1074.39 1007.86
Standard Deviation 103.83 130.61 132.92 169.69 101.31 112.43 133.02 139.79 107.42 122.65 135.05 147.98
Intensity Change Log 2 — -0.041909 0.275617 0.149939 — 0.169104 0.466250 0.325094 — 0.063360 0.370382 0.236775

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000038 0.000914 0.000199 0.001604 0.000043 0.000106 0.000119 0.000130 0.000041 0.000392 0.000183 0.000454
Bud Neck 0.000141 0.000993 0.000848 0.005427 0.000308 0.000265 0.000889 0.001167 0.000236 0.000523 0.000856 0.002102
Bud Site 0.000222 0.001518 0.000727 0.007431 0.000153 0.000366 0.000144 0.000322 0.000183 0.000773 0.000611 0.001883
Cell Periphery 0.000081 0.000249 0.000106 0.000274 0.000096 0.000101 0.000027 0.000060 0.000089 0.000154 0.000090 0.000107
Cytoplasm 0.028628 0.042050 0.019299 0.058108 0.034653 0.021190 0.005308 0.017950 0.032060 0.028568 0.016500 0.026771
Cytoplasmic Foci 0.003917 0.015544 0.000872 0.009477 0.004099 0.005307 0.000297 0.001667 0.004021 0.008928 0.000757 0.003383
Eisosomes 0.000009 0.000025 0.000007 0.000033 0.000007 0.000011 0.000003 0.000010 0.000008 0.000016 0.000007 0.000015
Endoplasmic Reticulum 0.295233* 0.228516* 0.169631* 0.173226* 0.253979* 0.249390* 0.107752* 0.130835* 0.271733* 0.242007* 0.157255* 0.140147*
Endosome 0.003069 0.014760 0.004903 0.022426 0.003869 0.005644 0.002017 0.006040 0.003525 0.008868 0.004326 0.009639
Golgi 0.001608 0.004173 0.000067 0.001427 0.000439 0.002036 0.000031 0.000464 0.000942 0.002792 0.000060 0.000676
Lipid Particles 0.021846 0.020994 0.001895 0.007600 0.014465 0.013622 0.001101 0.003475 0.017642 0.016229 0.001736 0.004381
Mitochondria 0.002877 0.008563 0.000969 0.002194 0.003837 0.005714 0.000730 0.000766 0.003424 0.006722 0.000922 0.001079
Mitotic Spindle 0.000942 0.003947 0.001025 0.009685 0.000600 0.001484 0.000410 0.007631 0.000747 0.002355 0.000902 0.008082
None 0.002425 0.006773 0.000936 0.001772 0.002974 0.001719 0.000097 0.000501 0.002738 0.003507 0.000768 0.000780
Nuclear Periphery 0.495110* 0.309563* 0.598359* 0.270387* 0.525413* 0.491742* 0.635613* 0.543340* 0.512372* 0.427306* 0.605810* 0.483382*
Nuclear Periphery Foci 0.119702 0.271421* 0.154845 0.299043* 0.125438 0.163859 0.205833* 0.222445* 0.122969 0.201903* 0.165043 0.239270*
Nucleolus 0.001227 0.006143 0.001180 0.004926 0.001143 0.001248 0.001891 0.002490 0.001179 0.002980 0.001322 0.003025
Nucleus 0.016253 0.036879 0.032644 0.052563 0.020032 0.021945 0.028178 0.034221 0.018405 0.027227 0.031751 0.038250
Peroxisomes 0.000340 0.001508 0.000111 0.002113 0.000283 0.000407 0.000119 0.000150 0.000307 0.000796 0.000112 0.000581
Vacuole 0.004854 0.016703 0.010671 0.063482 0.006357 0.009595 0.008815 0.023298 0.005711 0.012110 0.010300 0.032125
Vacuole Periphery 0.001479 0.008764 0.000706 0.006803 0.001811 0.004247 0.000625 0.003038 0.001668 0.005845 0.000690 0.003865

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.05 -5.92 -3.83 -1.48 -3.47 -4.09 -3.18 -3.86 -1.73 -0.35 -2.31 -6.40 -4.45 -0.74 -2.97
Bud Neck -5.46 -6.36 -4.58 -4.00 -4.07 0.55 -1.71 -3.10 -3.22 -0.51 -3.75 -4.55 -5.33 -4.78 -3.70
Bud Site -4.82 -2.05 -3.78 -3.21 -3.52 -2.14 -0.93 -3.03 -0.96 -2.05 -5.04 -2.36 -4.06 -2.93 -2.97
Cell Periphery -8.01 -2.17 -4.33 -1.23 -3.71 -0.53 6.94 2.50 2.88 -3.53 -6.11 -0.56 -2.15 1.84 -1.51
Cytoplasm -3.42 3.20 -2.85 -1.76 -3.53 5.10 10.53 3.71 0.08 -4.04 1.47 7.18 0.26 -0.52 -3.39
Cytoplasmic Foci -6.38 4.48 -2.51 1.80 -3.79 -1.21 6.57 3.32 3.92 -4.21 -5.43 7.16 0.42 5.17 -4.52
Eisosomes -5.70 0.39 -3.99 -1.70 -4.06 -2.50 2.94 -2.07 -0.43 -3.20 -5.60 0.33 -3.92 -0.69 -3.89
Endoplasmic Reticulum 6.42 14.47 7.09 3.37 0.32 0.55 14.61 12.89 11.64 -1.86 4.47 18.02 15.96 11.81 2.40
Endosome -8.19 -4.23 -4.94 -1.88 -4.44 -3.35 4.80 -3.04 -0.89 -5.18 -8.73 -2.43 -5.81 -0.93 -4.88
Golgi -2.32 2.78 0.24 2.32 -1.85 -3.24 4.16 -0.09 2.75 -1.44 -3.53 3.58 0.74 3.92 -2.15
Lipid Particles 0.14 12.02 5.98 5.34 -3.88 0.47 12.90 9.38 7.86 -4.57 0.84 16.98 12.17 10.24 -4.98
Mitochondria -6.23 3.68 0.90 6.92 -2.38 -1.91 4.84 4.94 6.23 0.19 -4.73 6.00 5.33 9.53 -1.20
Mitotic Spindle -3.05 -0.25 -1.83 -1.27 -1.81 -2.05 -0.18 -2.64 -2.34 -2.62 -3.57 -0.63 -3.17 -2.53 -3.05
None -3.39 2.80 0.90 3.73 -1.27 2.11 7.27 5.83 2.49 -1.99 -1.36 5.25 5.61 4.91 -0.08
Nuclear Periphery 16.00* -9.01 10.19 2.33 14.25 3.37 -6.16 -0.29 -2.44 4.74 10.93 -10.18 3.19 -2.99 9.06
Nuclear Periphery Foci -16.94* -5.79 -7.83 -0.96 -6.18 -6.56 -6.72 -9.24 -5.38 -1.26 -15.80 -8.09 -12.12 -3.58 -7.20
Nucleolus -5.57 0.09 -3.18 0.97 -3.27 -0.38 -1.15 -1.81 -1.73 -0.36 -4.88 -0.60 -2.79 -0.23 -2.38
Nucleus -5.59 -5.51 -3.66 -1.86 -2.15 -0.85 -2.35 -3.49 -2.99 -0.41 -4.36 -5.63 -5.09 -3.12 -1.69
Peroxisomes -3.24 2.98 -1.42 -0.27 -1.61 -1.72 2.75 1.89 3.82 -0.91 -3.58 4.09 -1.00 0.97 -1.70
Vacuole -7.23 -5.80 -6.47 -5.31 -5.78 -2.85 -1.80 -5.29 -4.46 -4.10 -6.93 -5.43 -8.03 -6.38 -6.48
Vacuole Periphery -6.99 2.60 -2.99 1.24 -3.51 -4.21 6.03 -1.12 1.03 -2.25 -7.91 5.56 -2.29 2.02 -3.40
Standard name
Human Ortholog
Description Protein of unknown function may be involved in glutathione metabolism; function suggested by computational analysis of large-scale protein-protein interaction data; N- and C-terminal fusion proteins localize to the cell periphery
Localization
Cell Percentages nuclear periphery (22%), ER (22%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gtt3

Gtt3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gtt3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available