Standard name
Human Ortholog
Description Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; glycogen accumulation defect of the null mutant is functionally complemented by human GBE1, which is associated with glycogen storage disease
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0.06 0.09 0.14 0.1 – 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.05 0.08 – 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.97 0.95 1.0 1.0 0.95 0.77 0.73 0.71 0.46 – 0.43 0.43 0.97 0.84 0.75 0.97 0.94 0.97 0.85 0.88 0.79 0.88 0.77 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.14 0.12 0.52 – 0.47 0.42 0 0 0 0 0 0 0.07 0 0.1 0.08 0.11 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0.12 0.12 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.09 0.06 0.05 0.1 – 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 9 14 24 15 – 15 23 0 3 4 0 0 0 0 1 0 0 3 1
Bud 0 0 0 0 1 5 7 9 13 – 16 22 2 3 7 0 0 0 0 1 0 0 2 3
Bud Neck 0 0 2 0 4 2 2 4 2 – 1 6 0 0 2 3 1 0 0 1 0 0 2 1
Bud Site 0 0 0 0 0 1 3 2 3 – 0 0 0 2 2 0 0 0 – – – – – –
Cell Periphery 0 0 6 2 7 0 2 4 5 – 6 6 0 2 3 11 8 13 0 0 1 0 3 1
Cytoplasm 210 275 93 96 135 108 113 118 71 – 78 91 268 258 287 524 299 187 198 251 80 61 202 198
Endoplasmic Reticulum 0 2 0 1 0 2 3 1 2 – 5 3 2 0 2 30 20 16 1 0 0 0 2 4
Endosome 1 4 0 0 0 0 1 2 0 – 1 1 3 9 32 5 6 0 3 3 0 0 2 1
Golgi 0 0 0 0 0 0 0 3 1 – 0 2 0 0 3 4 2 1 2 3 0 0 0 1
Mitochondria 3 5 0 0 0 18 21 20 80 – 85 88 4 6 15 5 4 0 15 7 10 5 29 9
Nucleus 1 1 1 1 0 1 2 3 2 – 1 6 0 0 1 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 1 0 0 0 – 1 0 0 1 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 2 0 0 0 1 0 1 0 – 2 3 0 1 2 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 3 2 1 2 – 2 0 1 36 47 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 5 13 9 9 16 – 5 10 1 9 22 0 1 0 1 3 1 0 2 1
Vac/Vac Membrane 2 3 0 0 0 0 0 3 0 – 12 13 1 2 4 9 10 5 1 3 1 1 5 8
Unique Cell Count 216 288 93 96 142 140 154 167 155 180 212 277 306 381 540 318 192 233 285 102 70 262 238
Labelled Cell Count 217 294 102 100 153 164 179 204 212 230 274 282 332 433 592 351 222 233 285 102 70 262 238


Cytoplasm, Nucleus, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.3 4.4 3.8 4.3 3.5 4.0 4.7 3.9 – 4.5 5.4 3.9 4.1 4.6 6.3 6.6 6.5 3.8 3.8 4.1
Std Deviation (1e-4) 0.6 1.0 0.9 0.7 0.7 1.0 0.9 1.2 1.1 – 1.5 1.7 1.1 1.0 1.8 1.3 1.9 1.7 1.5 1.3 1.3
Intensity Change (Log2) – – – -0.2 -0.01 -0.3 -0.12 0.11 -0.16 – 0.06 0.3 -0.17 -0.1 0.08 0.53 0.59 0.59 -0.21 -0.21 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0375 0.5538 -0.2187 0.0217 0.4086 0.7245 0.0008 0.7832 0.6958 0.7437 0.3632 0.4905 0.2062 1.0725 0.46 1.9658 0.0358 0.6021
Actin 0.0615 0.0058 0.0172 0.0024 0.0094 0.0023 0.0473 0.0057 0.0277 0.009 0.0051 0.0083 0.0444 0.0017 0.0056 0.0204 0.0206 0.0319
Bud 0.0013 0.0077 0.009 0.0008 0.0057 0.0068 0.0013 0.0061 0.0028 0.0006 0.0008 0.0018 0.008 0.0071 0.0029 0.0064 0.0011 0.0011
Bud Neck 0.001 0.0004 0.0006 0.0006 0.0015 0.0042 0.0051 0.0024 0.0029 0.0026 0.0011 0.0013 0.0033 0.0004 0.0012 0.0013 0.0057 0.0012
Bud Periphery 0.0011 0.0027 0.0046 0.0012 0.0075 0.0039 0.0013 0.0101 0.0041 0.0007 0.0011 0.003 0.0195 0.013 0.0034 0.0096 0.0011 0.0012
Bud Site 0.0023 0.0106 0.0038 0.0021 0.0285 0.0011 0.0047 0.0427 0.0289 0.0043 0.0045 0.0004 0.0134 0.025 0.0072 0.0107 0.0161 0.0044
Cell Periphery 0.0003 0.0003 0.0005 0.0003 0.0005 0.0007 0.0002 0.0008 0.0014 0.0006 0.0002 0.0001 0.0007 0.0004 0.0003 0.0013 0.0004 0.0002
Cytoplasm 0.3356 0.5582 0.479 0.5614 0.4869 0.5086 0.3438 0.4036 0.384 0.401 0.4687 0.3772 0.275 0.4339 0.4015 0.3623 0.4307 0.4387
Cytoplasmic Foci 0.0323 0.0488 0.0247 0.0072 0.0148 0.0342 0.0215 0.029 0.0332 0.0447 0.0369 0.0567 0.0166 0.0126 0.0539 0.0317 0.015 0.0429
Eisosomes 0.0016 0.0001 0.0002 0.0001 0.0002 0 0.0003 0.0002 0.0003 0.0005 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0066 0.0016 0.0062 0.0061 0.005 0.0045 0.0036 0.0023 0.0037 0.0075 0.0022 0.001 0.0142 0.0013 0.0016 0.0039 0.0029 0.0014
Endosome 0.0318 0.005 0.0049 0.042 0.0258 0.0166 0.0265 0.0031 0.0223 0.0403 0.0197 0.0064 0.0159 0.0032 0.0085 0.0176 0.0143 0.0129
Golgi 0.0074 0.0009 0.0026 0.0092 0.0111 0.0084 0.0124 0.0013 0.0166 0.0133 0.0092 0.0046 0.0037 0.0002 0.0051 0.0107 0.005 0.0021
Lipid Particles 0.0085 0.0023 0.0009 0.0037 0.0095 0.0208 0.009 0.0009 0.0148 0.0367 0.0078 0.0044 0.0093 0.0011 0.0051 0.0073 0.0036 0.0131
Mitochondria 0.0043 0.001 0.0012 0.017 0.1084 0.0036 0.0024 0.002 0.0165 0.038 0.0015 0.0107 0.0035 0.0009 0.0057 0.0286 0.0025 0.001
None 0.4441 0.3421 0.4307 0.3234 0.2549 0.334 0.4997 0.4763 0.3998 0.3382 0.4264 0.4933 0.5159 0.4683 0.4822 0.4326 0.4618 0.433
Nuclear Periphery 0.0121 0.0007 0.0016 0.0026 0.004 0.0015 0.0041 0.0006 0.0022 0.0021 0.0016 0.0004 0.0091 0.0008 0.0024 0.0021 0.0038 0.0008
Nucleolus 0.0054 0.0005 0.0005 0.0005 0.0013 0.0051 0.0004 0.0011 0.0005 0.0062 0.0003 0.0108 0.0027 0.0116 0.0008 0.002 0.0012 0.0008
Nucleus 0.0085 0.0026 0.0029 0.0028 0.0056 0.006 0.0023 0.002 0.0018 0.0072 0.0018 0.0043 0.0139 0.003 0.0025 0.0029 0.0037 0.0031
Peroxisomes 0.0148 0.0062 0.0014 0.0007 0.0073 0.0012 0.0073 0.0069 0.0121 0.0149 0.0048 0.0097 0.012 0.0006 0.0035 0.0334 0.0023 0.0068
Punctate Nuclear 0.0156 0.0013 0.0015 0.0011 0.0037 0.0043 0.0042 0.0014 0.0032 0.0239 0.0046 0.005 0.0101 0.0019 0.0032 0.0012 0.0057 0.0013
Vacuole 0.0024 0.0012 0.0046 0.0112 0.0071 0.0231 0.0017 0.0014 0.013 0.0055 0.0012 0.0006 0.0069 0.0103 0.0014 0.0097 0.0017 0.0015
Vacuole Periphery 0.0014 0.0001 0.0011 0.0033 0.0016 0.009 0.0007 0.0002 0.0084 0.0021 0.0003 0.0001 0.0017 0.0027 0.0019 0.0041 0.0005 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.912 7.4619 8.3056 19.7091 9.1316 23.4637 12.6139 21.9602 30.9834 14.312
Translational Efficiency 0.7213 0.5647 0.8593 0.939 1.0388 0.7998 0.7543 0.5777 0.7987 0.8328

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1830 1192 345 1228 1688 1579 373 65 3518 2771 718 1293

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 616.07 734.56 1570.92 834.02 665.47 734.05 945.91 877.44 639.77 734.27 1246.23 836.20
Standard Deviation 65.75 93.32 369.40 143.22 80.36 299.36 125.45 169.05 77.17 234.12 413.83 144.94
Intensity Change Log 2 — 0.253786 1.350444 0.436988 — 0.141505 0.507329 0.398927 — 0.196573 0.973729 0.417349

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000480 0.000335 0.265381* 0.017118 0.000475 0.000481 0.047658 0.003782 0.000477 0.000418 0.152274* 0.016447
Bud Neck 0.021432 0.031207 0.039978 0.015719 0.009713 0.039192 0.006641 0.004906 0.015809 0.035757 0.022659 0.015175
Bud Site 0.003792 0.019735 0.306343* 0.085674 0.002763 0.017891 0.038999 0.034987 0.003298 0.018685 0.167458* 0.083126
Cell Periphery 0.000474 0.000221 0.005913 0.000418 0.000118 0.000187 0.001142 0.000506 0.000303 0.000202 0.003434 0.000423
Cytoplasm 0.488411* 0.410776* 0.042634 0.379682* 0.502134* 0.370660* 0.222329* 0.350411* 0.494995* 0.387917* 0.135986* 0.378210*
Cytoplasmic Foci 0.213278* 0.271749* 0.083255 0.031856 0.210758* 0.317359* 0.104921 0.068391 0.212069* 0.297739* 0.094510 0.033693
Eisosomes 0.000565 0.000154 0.000986 0.000161 0.000122 0.000221 0.000784 0.000281 0.000353 0.000193 0.000881 0.000167
Endoplasmic Reticulum 0.000643 0.000593 0.015300 0.002980 0.000535 0.000517 0.007976 0.002679 0.000591 0.000550 0.011495 0.002965
Endosome 0.010007 0.019849 0.027622 0.013366 0.008232 0.030463 0.010565 0.008268 0.009155 0.025897 0.018761 0.013109
Golgi 0.001805 0.004474 0.026490 0.004302 0.001070 0.007945 0.001784 0.008470 0.001452 0.006452 0.013655 0.004512
Lipid Particles 0.011129 0.006807 0.008884 0.002550 0.006972 0.009388 0.012519 0.004252 0.009134 0.008278 0.010772 0.002636
Mitochondria 0.004074 0.008886 0.003995 0.010671 0.002820 0.016371 0.000405 0.003753 0.003472 0.013151 0.002130 0.010323
Mitotic Spindle 0.000780 0.006990 0.061793 0.065054 0.000362 0.010236 0.019071 0.007005 0.000579 0.008840 0.039599 0.062136
None 0.006623 0.002689 0.000197 0.003896 0.004759 0.001818 0.002339 0.002729 0.005728 0.002193 0.001310 0.003838
Nuclear Periphery 0.000431 0.000398 0.000447 0.001430 0.000530 0.000309 0.001576 0.002217 0.000478 0.000347 0.001034 0.001470
Nuclear Periphery Foci 0.000303 0.000374 0.002354 0.001206 0.000333 0.000246 0.005613 0.002327 0.000318 0.000301 0.004047 0.001262
Nucleolus 0.001912 0.002055 0.000358 0.000497 0.001018 0.001412 0.000930 0.002623 0.001483 0.001689 0.000655 0.000604
Nucleus 0.187601* 0.120820 0.018680 0.223214* 0.207101* 0.083559 0.304332* 0.301832* 0.196957* 0.099588 0.167076* 0.227166*
Peroxisomes 0.004414 0.020535 0.032709 0.013778 0.004008 0.023949 0.067069 0.003235 0.004219 0.022480 0.050559 0.013248
Vacuole 0.041034 0.069372 0.053253 0.120946 0.035495 0.065198 0.143138 0.178664 0.038376 0.066994 0.099948 0.123848
Vacuole Periphery 0.000812 0.001981 0.003429 0.005481 0.000684 0.002593 0.000211 0.008683 0.000751 0.002330 0.001757 0.005642

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.47 -12.47 -4.50 -4.69 11.99 -0.02 -4.71 -1.46 -1.50 4.19 0.24 -12.40 -4.60 -4.70 11.51
Bud Neck -3.41 -5.57 3.67 6.54 7.61 -12.50 2.02 -0.22 6.19 -0.89 -10.83 -4.07 1.20 11.21 4.71
Bud Site -7.48 -19.23 -11.51 -6.70 15.43* -8.41 -5.12 -1.89 -0.85 -0.25 -11.23 -16.68 -11.82 -7.38 10.44
Cell Periphery 1.08 -4.04 0.84 -1.40 4.31 -3.33 -4.07 -1.78 -1.32 2.06 0.85 -4.59 0.17 -3.12 4.75
Cytoplasm 8.27 44.77 20.83 10.19 -21.60 13.88 23.27 6.80 2.59 -2.69 16.03 42.43 23.95 10.18 -15.46
Cytoplasmic Foci -7.46 15.13 39.76 35.94 6.77 -13.51 15.11 12.36 20.27 1.46 -15.37 21.46 49.17 56.58 9.36
Eisosomes 1.83 -0.75 1.92 1.83 6.17 -8.01 -3.33 -1.28 -0.18 1.95 1.37 -2.02 1.82 4.57 6.17
Endoplasmic Reticulum 0.36 -5.53 -10.46 -10.54 3.93 0.22 -10.67 -5.68 -5.64 6.17 0.50 -9.08 -11.18 -11.17 5.86
Endosome -6.92 -5.22 3.55 8.89 6.70 -12.52 -1.12 1.44 10.87 2.04 -14.13 -4.90 3.23 14.07 6.38
Golgi -4.03 -4.93 0.84 4.05 5.01 -10.12 -0.13 -0.77 1.53 -0.76 -10.36 -4.81 -0.15 7.79 4.65
Lipid Particles 4.81 -0.35 10.99 7.97 6.90 -2.59 -3.34 3.91 5.76 5.76 1.28 -2.32 12.89 10.99 9.99
Mitochondria -3.91 1.01 0.17 3.19 -0.43 -8.00 3.58 0.94 7.45 -0.84 -8.86 3.41 -0.28 6.54 -1.87
Mitotic Spindle -3.53 -7.55 -10.31 -8.54 2.26 -5.72 -3.23 -1.94 1.28 1.74 -6.64 -8.02 -10.41 -8.33 -0.57
None 4.24 7.02 2.63 -2.89 -9.43 3.00 2.44 1.29 -1.00 -0.59 5.28 6.47 2.27 -4.38 -6.24
Nuclear Periphery 0.36 -4.81 -17.13 -18.03 -10.09 3.04 -13.24 -4.88 -5.50 0.07 2.56 -12.80 -17.94 -19.00 -6.73
Nuclear Periphery Foci -1.20 -8.12 -7.75 -7.61 2.97 1.87 -4.83 -4.71 -4.92 3.20 0.37 -6.60 -8.29 -8.43 4.13
Nucleolus -0.31 3.40 -0.66 -0.51 -2.48 -1.29 -0.67 -1.39 -1.26 -1.21 -0.89 0.64 -1.60 -1.19 -1.74
Nucleus 11.45 14.07 -10.87 -18.23 -19.67 21.72 -7.81 -2.07 -5.97 1.70 24.35 -0.41 -10.31 -23.20 -6.05
Peroxisomes -9.51 -5.26 -2.03 6.92 4.47 -12.46 -5.67 -0.18 8.25 5.44 -15.55 -7.48 -2.35 10.03 6.59
Vacuole -9.43 -6.48 -25.97 -20.83 -12.02 -10.94 -14.76 -7.67 -6.73 -1.90 -14.26 -15.23 -27.52 -23.01 -7.43
Vacuole Periphery -3.00 -1.87 -1.66 0.23 0.80 -5.51 5.23 -0.83 -0.20 -0.99 -6.13 -1.34 -1.93 0.73 -0.58
Standard name
Human Ortholog
Description Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; glycogen accumulation defect of the null mutant is functionally complemented by human GBE1, which is associated with glycogen storage disease
Localization
Cell Percentages cytoplasm (18%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Glc3

Glc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available