Standard name
Description bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0.07 0.13 0.11 0.15 0.2 0.25 0.24 0.27 0.29 0.31 – 0.08 0.11 0.1 0.1 0.15 0.06 0 0 0.05 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome – 0 0 0 0 0.05 0.05 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.31 0 0.07 0 0.17 0.2 0.25 0.67 0.53 0.63 0.58 – 0 0 0.1 0 0 0 0 0 0.07 0.14 0.12
Nucleus – 0.88 0.91 0.8 0.79 0.61 0.57 0.43 0.29 0.31 0.23 0.21 – 0.88 0.72 0.79 0.82 0.78 0.69 0.87 0.82 0.58 0.43 0.29
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0.1
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0.05 0.06 0 0.06 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane – 0 0 0 0 0.11 0.05 0.11 0 0.05 0 0.05 – 0.05 0.07 0.08 0.06 0.08 0.1 0.05 0.06 0.12 0.17 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 2 0 1
Bud – 0 0 0 1 2 4 4 12 2 12 17 – 0 0 0 0 0 0 0 2 1 1 4
Bud Neck – 0 0 0 1 1 3 6 3 5 5 7 – 0 0 0 0 0 0 0 0 0 0 2
Bud Site – 1 0 1 1 1 2 2 8 2 3 3 – 0 0 0 0 0 – – – – – –
Cell Periphery – 0 1 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm – 5 26 31 32 48 69 108 86 106 107 119 – 5 13 15 13 19 2 2 5 3 1 3
Endoplasmic Reticulum – 0 1 0 0 0 0 0 0 0 0 0 – 0 0 7 3 6 1 0 1 0 1 1
Endosome – 0 2 4 7 16 16 13 3 7 4 3 – 0 7 4 4 1 0 3 1 0 3 3
Golgi – 0 0 1 0 0 0 2 0 0 0 0 – 0 0 1 0 0 0 0 1 0 2 4
Mitochondria – 50 3 17 8 55 69 109 237 207 234 225 – 0 4 15 4 2 1 0 8 4 19 15
Nucleus – 143 326 193 235 193 192 186 105 120 85 82 – 56 83 120 103 99 24 145 303 36 62 35
Nuclear Periphery – 2 2 0 1 0 0 0 1 2 0 1 – 0 1 6 1 0 0 1 0 1 3 1
Nucleolus – 0 0 8 8 10 4 2 1 2 4 6 – 0 3 2 3 1 1 1 10 2 12 11
Peroxisomes – 0 0 0 0 2 0 0 0 0 0 0 – 0 1 0 0 0 0 0 1 0 3 0
SpindlePole – 0 1 4 8 10 17 26 11 22 19 9 – 1 2 0 1 1 0 0 5 0 3 5
Vac/Vac Membrane – 5 4 8 13 36 17 48 13 19 15 21 – 3 8 12 7 10 3 8 20 7 25 21
Unique Cell Count 163 359 241 296 319 337 431 356 392 371 390 64 116 152 126 127 35 167 371 62 146 123
Labelled Cell Count 206 366 267 315 374 393 506 480 494 488 493 65 122 182 139 140 35 167 371 62 146 123


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.4 6.8 6.4 6.4 5.1 4.7 4.9 3.6 4.0 3.7 3.7 – 6.4 6.6 5.5 7.3 8.1 5.4 7.0 6.8
Std Deviation (1e-4) – 0.7 1.6 1.9 2.0 1.8 1.5 1.5 1.3 1.4 1.2 0.8 – 0.9 6.6 1.2 1.5 1.8 0.5 1.9 2.3
Intensity Change (Log2) – – – -0.09 -0.08 -0.42 -0.52 -0.46 -0.89 -0.74 -0.86 -0.87 – -0.08 -0.04 -0.31 0.11 0.25 -0.32 0.05 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –