Standard name | |
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Description | bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 |
Bud | – | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 12 | 2 | 12 | 17 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 4 |
Bud Neck | – | 0 | 0 | 0 | 1 | 1 | 3 | 6 | 3 | 5 | 5 | 7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | – | 1 | 0 | 1 | 1 | 1 | 2 | 2 | 8 | 2 | 3 | 3 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 5 | 26 | 31 | 32 | 48 | 69 | 108 | 86 | 106 | 107 | 119 | – | 5 | 13 | 15 | 13 | 19 | 2 | 2 | 5 | 3 | 1 | 3 |
Endoplasmic Reticulum | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 7 | 3 | 6 | 1 | 0 | 1 | 0 | 1 | 1 |
Endosome | – | 0 | 2 | 4 | 7 | 16 | 16 | 13 | 3 | 7 | 4 | 3 | – | 0 | 7 | 4 | 4 | 1 | 0 | 3 | 1 | 0 | 3 | 3 |
Golgi | – | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 |
Mitochondria | – | 50 | 3 | 17 | 8 | 55 | 69 | 109 | 237 | 207 | 234 | 225 | – | 0 | 4 | 15 | 4 | 2 | 1 | 0 | 8 | 4 | 19 | 15 |
Nucleus | – | 143 | 326 | 193 | 235 | 193 | 192 | 186 | 105 | 120 | 85 | 82 | – | 56 | 83 | 120 | 103 | 99 | 24 | 145 | 303 | 36 | 62 | 35 |
Nuclear Periphery | – | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | – | 0 | 1 | 6 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 1 |
Nucleolus | – | 0 | 0 | 8 | 8 | 10 | 4 | 2 | 1 | 2 | 4 | 6 | – | 0 | 3 | 2 | 3 | 1 | 1 | 1 | 10 | 2 | 12 | 11 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 |
SpindlePole | – | 0 | 1 | 4 | 8 | 10 | 17 | 26 | 11 | 22 | 19 | 9 | – | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 5 | 0 | 3 | 5 |
Vac/Vac Membrane | – | 5 | 4 | 8 | 13 | 36 | 17 | 48 | 13 | 19 | 15 | 21 | – | 3 | 8 | 12 | 7 | 10 | 3 | 8 | 20 | 7 | 25 | 21 |
Unique Cell Count | 163 | 359 | 241 | 296 | 319 | 337 | 431 | 356 | 392 | 371 | 390 | 64 | 116 | 152 | 126 | 127 | 35 | 167 | 371 | 62 | 146 | 123 | ||
Labelled Cell Count | 206 | 366 | 267 | 315 | 374 | 393 | 506 | 480 | 494 | 488 | 493 | 65 | 122 | 182 | 139 | 140 | 35 | 167 | 371 | 62 | 146 | 123 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 4.4 | 6.8 | 6.4 | 6.4 | 5.1 | 4.7 | 4.9 | 3.6 | 4.0 | 3.7 | 3.7 | – | 6.4 | 6.6 | 5.5 | 7.3 | 8.1 | 5.4 | 7.0 | 6.8 |
Std Deviation (1e-4) | – | 0.7 | 1.6 | 1.9 | 2.0 | 1.8 | 1.5 | 1.5 | 1.3 | 1.4 | 1.2 | 0.8 | – | 0.9 | 6.6 | 1.2 | 1.5 | 1.8 | 0.5 | 1.9 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.08 | -0.42 | -0.52 | -0.46 | -0.89 | -0.74 | -0.86 | -0.87 | – | -0.08 | -0.04 | -0.31 | 0.11 | 0.25 | -0.32 | 0.05 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |