Standard name | |
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Human Ortholog | |
Description | bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 |
Bud | – | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 12 | 2 | 12 | 17 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 4 |
Bud Neck | – | 0 | 0 | 0 | 1 | 1 | 3 | 6 | 3 | 5 | 5 | 7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | – | 1 | 0 | 1 | 1 | 1 | 2 | 2 | 8 | 2 | 3 | 3 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 5 | 26 | 31 | 32 | 48 | 69 | 108 | 86 | 106 | 107 | 119 | – | 5 | 13 | 15 | 13 | 19 | 2 | 2 | 5 | 3 | 1 | 3 |
Endoplasmic Reticulum | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 7 | 3 | 6 | 1 | 0 | 1 | 0 | 1 | 1 |
Endosome | – | 0 | 2 | 4 | 7 | 16 | 16 | 13 | 3 | 7 | 4 | 3 | – | 0 | 7 | 4 | 4 | 1 | 0 | 3 | 1 | 0 | 3 | 3 |
Golgi | – | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 4 |
Mitochondria | – | 50 | 3 | 17 | 8 | 55 | 69 | 109 | 237 | 207 | 234 | 225 | – | 0 | 4 | 15 | 4 | 2 | 1 | 0 | 8 | 4 | 19 | 15 |
Nucleus | – | 143 | 326 | 193 | 235 | 193 | 192 | 186 | 105 | 120 | 85 | 82 | – | 56 | 83 | 120 | 103 | 99 | 24 | 145 | 303 | 36 | 62 | 35 |
Nuclear Periphery | – | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | – | 0 | 1 | 6 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 1 |
Nucleolus | – | 0 | 0 | 8 | 8 | 10 | 4 | 2 | 1 | 2 | 4 | 6 | – | 0 | 3 | 2 | 3 | 1 | 1 | 1 | 10 | 2 | 12 | 11 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 |
SpindlePole | – | 0 | 1 | 4 | 8 | 10 | 17 | 26 | 11 | 22 | 19 | 9 | – | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 5 | 0 | 3 | 5 |
Vac/Vac Membrane | – | 5 | 4 | 8 | 13 | 36 | 17 | 48 | 13 | 19 | 15 | 21 | – | 3 | 8 | 12 | 7 | 10 | 3 | 8 | 20 | 7 | 25 | 21 |
Unique Cell Count | 163 | 359 | 241 | 296 | 319 | 337 | 431 | 356 | 392 | 371 | 390 | 64 | 116 | 152 | 126 | 127 | 35 | 167 | 371 | 62 | 146 | 123 | ||
Labelled Cell Count | 206 | 366 | 267 | 315 | 374 | 393 | 506 | 480 | 494 | 488 | 493 | 65 | 122 | 182 | 139 | 140 | 35 | 167 | 371 | 62 | 146 | 123 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 4.4 | 6.8 | 6.4 | 6.4 | 5.1 | 4.7 | 4.9 | 3.6 | 4.0 | 3.7 | 3.7 | – | 6.4 | 6.6 | 5.5 | 7.3 | 8.1 | 5.4 | 7.0 | 6.8 |
Std Deviation (1e-4) | – | 0.7 | 1.6 | 1.9 | 2.0 | 1.8 | 1.5 | 1.5 | 1.3 | 1.4 | 1.2 | 0.8 | – | 0.9 | 6.6 | 1.2 | 1.5 | 1.8 | 0.5 | 1.9 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.08 | -0.42 | -0.52 | -0.46 | -0.89 | -0.74 | -0.86 | -0.87 | – | -0.08 | -0.04 | -0.31 | 0.11 | 0.25 | -0.32 | 0.05 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.2705 | 7.373 | 7.4796 | 7.0507 | 7.6303 | 7.6193 | 8.164 | 10.1881 | 9.3733 | 7.2805 | 8.6779 | 8.796 | 5.2558 | 6.9195 | 6.3027 | 6.1836 | 6.1556 | 5.6639 |
Actin | 0.0136 | 0 | 0.0044 | 0 | 0 | 0.0167 | 0.085 | 0.0909 | 0.0023 | 0.0571 | 0.0007 | 0.0006 | 0.008 | 0 | 0.0145 | 0 | 0 | 0.0125 |
Bud | 0.0004 | 0.0007 | 0.0012 | 0.0001 | 0 | 0.0003 | 0.0013 | 0.0008 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.0009 | 0.0002 | 0.0006 | 0.0005 | 0.0005 | 0.0049 | 0.0027 | 0.0003 | 0.002 | 0.0003 | 0.0005 | 0.0012 | 0.0005 | 0.0003 | 0.0014 | 0.0004 | 0.0005 | 0.0014 |
Bud Periphery | 0.0008 | 0.0006 | 0.0031 | 0.0001 | 0.0001 | 0.0006 | 0.0025 | 0.0007 | 0.0001 | 0.0008 | 0.0001 | 0.0008 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0006 |
Bud Site | 0.0024 | 0.0014 | 0.0027 | 0.0001 | 0.0001 | 0.0014 | 0.0066 | 0.0043 | 0.0003 | 0.0025 | 0.0002 | 0.0005 | 0.0012 | 0.0005 | 0.0048 | 0.0002 | 0.0002 | 0.0008 |
Cell Periphery | 0.0005 | 0.0002 | 0.0004 | 0 | 0 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0004 |
Cytoplasm | 0.0019 | 0.0002 | 0.0005 | 0.0478 | 0.0001 | 0.002 | 0.0218 | 0.0026 | 0.004 | 0.005 | 0.041 | 0.0003 | 0.0112 | 0.0001 | 0.0211 | 0.0001 | 0 | 0.0023 |
Cytoplasmic Foci | 0.0128 | 0 | 0.0047 | 0.0002 | 0 | 0.0032 | 0.0075 | 0.0079 | 0.0104 | 0.0348 | 0.0033 | 0.0003 | 0.0078 | 0 | 0.017 | 0 | 0 | 0.0069 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0004 |
Endoplasmic Reticulum | 0.0016 | 0 | 0.0002 | 0.0003 | 0 | 0.0022 | 0.0039 | 0.0066 | 0.0018 | 0.0016 | 0.0052 | 0.0006 | 0.0037 | 0 | 0.0046 | 0 | 0 | 0.0011 |
Endosome | 0.012 | 0 | 0.0107 | 0.0008 | 0 | 0.0057 | 0.0131 | 0.0166 | 0.0201 | 0.0346 | 0.0391 | 0.0032 | 0.0166 | 0 | 0.0046 | 0.0001 | 0 | 0.0093 |
Golgi | 0.0043 | 0 | 0.0012 | 0 | 0 | 0.0012 | 0.0066 | 0.0194 | 0.0087 | 0.0366 | 0.0006 | 0.0005 | 0.0035 | 0 | 0.002 | 0 | 0 | 0.0088 |
Lipid Particles | 0.0118 | 0 | 0.0029 | 0 | 0 | 0.0027 | 0.003 | 0.0043 | 0.001 | 0.0035 | 0.0003 | 0.0004 | 0.0125 | 0 | 0.0043 | 0.0001 | 0 | 0.003 |
Mitochondria | 0.0048 | 0.0003 | 0.0021 | 0.0001 | 0.0002 | 0.0088 | 0.005 | 0.0051 | 0.0008 | 0.006 | 0.0018 | 0.007 | 0.0084 | 0.0003 | 0.0011 | 0.0006 | 0.0005 | 0.0043 |
None | 0.0038 | 0 | 0.0011 | 0.0003 | 0 | 0.0008 | 0.0049 | 0.0007 | 0.0001 | 0.0011 | 0.0039 | 0.0001 | 0.0106 | 0 | 0.0131 | 0 | 0 | 0.008 |
Nuclear Periphery | 0.0055 | 0.0024 | 0.0005 | 0.0005 | 0.0019 | 0.0025 | 0.0192 | 0.0033 | 0.0013 | 0.0344 | 0.0297 | 0.002 | 0.0169 | 0.0006 | 0.0099 | 0.0042 | 0.0005 | 0.0026 |
Nucleolus | 0.2299 | 0.1418 | 0.3088 | 0.3082 | 0.195 | 0.2256 | 0.0928 | 0.0101 | 0.3012 | 0.0116 | 0.1135 | 0.2421 | 0.1917 | 0.2173 | 0.2521 | 0.1578 | 0.2127 | 0.2418 |
Nucleus | 0.6672 | 0.8498 | 0.6482 | 0.6375 | 0.8013 | 0.7106 | 0.6755 | 0.8222 | 0.6437 | 0.7497 | 0.7488 | 0.7164 | 0.6748 | 0.7794 | 0.6335 | 0.8348 | 0.7846 | 0.6784 |
Peroxisomes | 0.0049 | 0 | 0.0029 | 0 | 0 | 0.0045 | 0.0029 | 0.0021 | 0.0005 | 0.0147 | 0.0002 | 0.0001 | 0.0014 | 0 | 0.0088 | 0 | 0 | 0.002 |
Punctate Nuclear | 0.0102 | 0.0006 | 0.0011 | 0.0025 | 0.0006 | 0.004 | 0.0353 | 0.0008 | 0.0006 | 0.0019 | 0.0067 | 0.0191 | 0.021 | 0.0004 | 0.0036 | 0.0003 | 0.0002 | 0.0017 |
Vacuole | 0.0085 | 0.0011 | 0.0023 | 0.0007 | 0.0001 | 0.0014 | 0.0061 | 0.0004 | 0.0005 | 0.0012 | 0.0028 | 0.0029 | 0.0053 | 0.0004 | 0.0014 | 0.0006 | 0.0003 | 0.009 |
Vacuole Periphery | 0.002 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0005 | 0.0031 | 0.0004 | 0.0003 | 0.0013 | 0.0016 | 0.0014 | 0.0028 | 0.0002 | 0.0014 | 0.0005 | 0.0001 | 0.0045 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 599.9599 | 664.5797 | 650.6077 | 569.8869 | 519.992 | 684.3577 | 719.9948 | 594.3563 | 621.1213 | 591.2013 |
Translational Efficiency | 0.2173 | 0.2367 | 0.1869 | 0.2134 | 0.2198 | 0.3864 | 0.2566 | 0.3193 | 0.2594 | 0.2068 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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