Standard name
Human Ortholog
Description Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.08 0.07 0.08
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.12 0 0.14 0.08 0.09 0.06 0.1 0.05 0 0.05 0.05 0.2 0.24 0.33 0.2 0.2 0.19 0 0 0 0 0.05 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Endosome 0.06 0 0.09 0 0.05 0 0 0 0 0 0 0 0 0.11 0.07 0.08 0.11 0.12 0 0 0.06 0 0 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.15 0 0.27 0.09 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.58 0.41 0.43 0.81 0.59 0.66 0.64 0.62 0.59 0.64 0.44 0.54 0.46 0.33 0.31 0.31 0.25 0.21 0.44 0.46 0.43 0.51 0.46 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.28 0.13 0.13 0 0.06 0 0.11 0.15 0.21 0.19 0.35 0.29 0 0 0 0 0 0 0.34 0.26 0.18 0.2 0.16 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0.07 0.09 0.1
Vac/Vac Membrane 0.14 0.4 0.4 0.05 0.25 0.19 0.19 0.13 0.1 0.17 0.08 0.14 0.36 0.42 0.41 0.45 0.49 0.54 0 0.07 0.1 0.05 0.08 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 1 0 0 3 0 1 1 1 0 0 0 0 0 0 10 12 9 10 13 21
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2 3
Bud Site 0 0 0 0 0 0 0 1 2 0 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 7 20 2 3 14 12 12 28 8 7 8 11 25 51 70 29 32 29 4 8 1 4 9 6
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 1 0 0 0 0 1 1 11 6 7 0 0 2 0 1 1
Endosome 15 5 5 0 9 5 7 5 2 2 0 3 4 23 15 12 18 19 17 9 6 2 8 21
Golgi 0 2 0 0 3 0 1 1 1 1 0 2 1 1 1 4 7 4 0 3 0 1 0 1
Mitochondria 2 2 0 0 0 3 1 6 23 6 39 19 1 1 0 7 1 3 0 2 0 0 0 0
Nucleus 152 69 23 17 102 89 118 182 92 102 64 116 57 70 65 45 40 33 185 118 45 67 92 109
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 2 1 0 0 2 1
Nucleolus 73 22 7 0 10 6 21 44 32 31 51 62 1 3 2 0 6 3 144 66 18 25 33 52
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 1 1 2 0 0 0 0 1 1 0 1 0 0 36 11 4 9 18 25
Vac/Vac Membrane 37 67 21 1 43 26 36 38 16 27 12 30 44 91 86 65 80 83 14 18 10 6 15 16
Unique Cell Count 260 168 53 21 173 134 185 293 156 160 146 213 123 215 212 145 162 155 422 254 106 132 203 266
Labelled Cell Count 287 188 59 21 182 142 198 311 178 177 176 246 134 242 240 174 191 182 422 254 106 132 203 266


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 13.2 10.4 11.0 11.3 9.8 10.5 10.7 10.0 11.1 9.1 10.0 11.5 11.3 11.6 11.6 12.0 11.5 10.5 11.0 11.6
Std Deviation (1e-4) 1.6 2.6 1.8 1.7 1.9 1.9 2.2 2.3 2.4 2.6 2.5 2.9 2.1 2.0 2.2 2.4 2.2 2.4 1.8 2.0 1.9
Intensity Change (Log2) – – – 0.08 0.12 -0.08 0.01 0.04 -0.06 0.1 -0.19 -0.05 0.14 0.12 0.15 0.16 0.21 0.15 0.02 0.08 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 2.8 3.3 4.3 2.8 2.8 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0.3 0 0 0.3 0.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.0 0 4.2 0 0 0 0 0 0 0
Nucleus 2.9 2.0 2.9 2.7 2.6 2.0 2.6 0.1 1.4 0.4 -1.5 -1.8 -1.6 -2.6 -3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 -0.4 0.3 1.2 1.0 3.0 2.4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.0 -2.1 -2.9 -3.0 -4.7 -4.8 -3.4 -5.3 -4.2 -0.5 0.4 0.1 0.7 1.2 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.5859 6.657 6.0418 5.9098 5.3855 6.1884 6.0028 6.7238 5.753 4.9746 4.7373 5.8405 9.3185 8.3215 7.9904 7.8842 7.8147 7.8891
Actin 0.0091 0.0001 0.0038 0 0.0145 0.0077 0.0009 0.0002 0.0003 0.0016 0.0061 0.0002 0.0002 0 0.0001 0.0004 0.0004 0
Bud 0.001 0.0001 0.0003 0.0001 0.0025 0.0002 0.0002 0.0005 0.0008 0.0002 0.0028 0.0002 0 0.0001 0 0.0006 0.0001 0.0001
Bud Neck 0.0021 0.0003 0.0009 0.0014 0.0377 0.0035 0.0006 0.0011 0.0005 0.0002 0.004 0.0037 0.0001 0.0004 0.0001 0.0009 0.0022 0.0016
Bud Periphery 0.0023 0 0.0005 0.0001 0.0038 0.0003 0.0005 0.0004 0.0004 0.0004 0.0081 0.0003 0 0 0 0.0004 0.0001 0.0001
Bud Site 0.0021 0.0004 0.0041 0.0001 0.0123 0.0003 0.0004 0.0013 0.0034 0.0001 0.0069 0.0004 0.0001 0.0005 0.0002 0.001 0.0004 0.0001
Cell Periphery 0.0007 0 0.0005 0 0.0006 0 0.0001 0 0 0 0.002 0.0001 0 0 0 0 0 0
Cytoplasm 0.0061 0.0022 0.0021 0.0003 0.0095 0.0018 0.0028 0.0035 0.0025 0.0002 0.0097 0.0031 0.0002 0.0044 0.0004 0.0002 0.0014 0.0003
Cytoplasmic Foci 0.0233 0.001 0.01 0 0.0341 0.0016 0.0061 0.0003 0.003 0.0003 0.0233 0.0046 0.0012 0.0001 0 0.0001 0.0028 0
Eisosomes 0.0001 0 0.0002 0 0.0002 0 0 0 0 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0022 0 0.0007 0 0.0057 0.0005 0.0006 0.0002 0.0003 0.0002 0.0039 0.0002 0 0.0001 0 0 0.0019 0
Endosome 0.0324 0.0001 0.0084 0 0.0686 0.0063 0.0047 0.0002 0.0019 0.0033 0.0215 0.0022 0.0011 0.0003 0.0001 0 0.0403 0.0001
Golgi 0.0109 0.0002 0.0029 0 0.0303 0.004 0.0004 0 0.0003 0.0036 0.0033 0.0013 0.0004 0 0 0 0.0034 0
Lipid Particles 0.0125 0.0033 0.0112 0 0.0312 0.0075 0.0013 0 0.0017 0.0025 0.0086 0.0083 0.0015 0 0 0 0.0043 0
Mitochondria 0.0067 0.0003 0.0032 0.0001 0.0227 0.0015 0.0083 0.0002 0.0003 0.0686 0.0167 0.001 0.0002 0.0001 0.0002 0.0002 0.0026 0.0001
None 0.0114 0 0.0005 0 0.0028 0.0002 0.002 0.0081 0.0326 0.0001 0.0451 0.0065 0.0001 0 0.0003 0.0001 0.0006 0
Nuclear Periphery 0.0072 0.0034 0.0043 0.002 0.0396 0.0054 0.0097 0.0047 0.0052 0.0021 0.0363 0.0028 0.0014 0.0056 0.0032 0.0017 0.0048 0.0022
Nucleolus 0.0211 0.0211 0.0213 0.0159 0.0627 0.0466 0.016 0.0146 0.0146 0.0055 0.0273 0.029 0.0104 0.0155 0.0157 0.015 0.044 0.028
Nucleus 0.8249 0.9582 0.9107 0.9786 0.5206 0.9058 0.9159 0.9518 0.9242 0.9082 0.7103 0.926 0.9802 0.9557 0.9727 0.9514 0.8658 0.954
Peroxisomes 0.0058 0.0052 0.008 0 0.0054 0.0019 0.0003 0 0.0002 0.0005 0.0027 0.0022 0.0003 0 0 0 0.0004 0
Punctate Nuclear 0.0106 0.0038 0.0044 0.0011 0.0857 0.0037 0.0269 0.0123 0.0075 0.0007 0.038 0.0068 0.0025 0.0163 0.0066 0.0279 0.0222 0.0132
Vacuole 0.0059 0.0001 0.0016 0.0001 0.0065 0.0008 0.0017 0.0003 0.0002 0.0004 0.0161 0.0009 0.0001 0.0005 0.0002 0 0.0014 0.0001
Vacuole Periphery 0.0015 0.0001 0.0004 0.0001 0.003 0.0003 0.0007 0.0001 0.0001 0.0011 0.007 0.0002 0 0.0003 0.0001 0 0.0009 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.8799 28.0977 24.5075 38.7657 23.9508 20.3915 36.7579 35.6579 33.6265 47.6101
Translational Efficiency 0.7752 0.9441 0.806 0.6051 1.231 1.4766 0.755 0.7432 0.9197 0.7541

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1970 895 82 675 1885 1878 106 2358 3855 2773 188 3033

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 807.09 1051.73 1997.78 1171.03 1046.61 1044.11 1510.21 1321.29 924.21 1046.57 1722.87 1287.85
Standard Deviation 104.02 339.53 267.02 203.95 158.37 169.33 249.54 194.74 179.24 237.99 353.09 206.51
Intensity Change Log 2 — 0.381963 1.307596 0.536977 — -0.003450 0.529025 0.336223 — 0.177121 0.920237 0.427082

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000692 0.002967 0.004140 0.001971 0.000817 0.000805 0.003413 0.000888 0.000753 0.001502 0.003730 0.001129
Bud Neck 0.011538 0.019747 0.033160 0.023262 0.024966 0.028394 0.008897 0.053038 0.018104 0.025603 0.019480 0.046411
Bud Site 0.002156 0.008070 0.009199 0.016292 0.001952 0.003521 0.004901 0.012789 0.002056 0.004989 0.006776 0.013569
Cell Periphery 0.000110 0.000324 0.000442 0.000342 0.000080 0.000090 0.000359 0.000194 0.000095 0.000166 0.000395 0.000227
Cytoplasm 0.057117 0.043555 0.003189 0.123471* 0.009712 0.014366 0.067240 0.066224 0.033937 0.023787 0.039303 0.078964
Cytoplasmic Foci 0.001677 0.001951 0.001621 0.002750 0.001164 0.000906 0.006185 0.000694 0.001426 0.001243 0.004194 0.001151
Eisosomes 0.000022 0.000042 0.000124 0.000026 0.000028 0.000030 0.000104 0.000020 0.000025 0.000034 0.000113 0.000022
Endoplasmic Reticulum 0.002366 0.006090 0.004251 0.003825 0.003625 0.002982 0.020633 0.002496 0.002982 0.003985 0.013488 0.002792
Endosome 0.000758 0.001128 0.000398 0.008469 0.000857 0.001738 0.003106 0.002262 0.000806 0.001541 0.001925 0.003643
Golgi 0.000554 0.001355 0.000576 0.008140 0.000563 0.000517 0.000409 0.004066 0.000558 0.000787 0.000482 0.004973
Lipid Particles 0.000313 0.000725 0.000670 0.000749 0.000273 0.000327 0.002301 0.000266 0.000293 0.000455 0.001589 0.000373
Mitochondria 0.001339 0.002462 0.005973 0.009209 0.001526 0.004943 0.001906 0.005528 0.001431 0.004142 0.003680 0.006347
Mitotic Spindle 0.003143 0.020444 0.007573 0.109377* 0.004613 0.019060 0.005556 0.220112* 0.003862 0.019507 0.006436 0.195468*
None 0.027783 0.017233 0.001872 0.020161 0.005099 0.006975 0.002538 0.013245 0.016691 0.010286 0.002247 0.014784
Nuclear Periphery 0.000751 0.000869 0.001609 0.002442 0.000872 0.001166 0.002129 0.009382 0.000810 0.001070 0.001902 0.007838
Nuclear Periphery Foci 0.004355 0.011518 0.000321 0.002551 0.002779 0.001883 0.006610 0.003144 0.003584 0.004993 0.003867 0.003012
Nucleolus 0.096699 0.121241 0.013526 0.003424 0.118248 0.131754 0.018084 0.015342 0.107236 0.128360 0.016096 0.012689
Nucleus 0.786824* 0.734491* 0.895529* 0.638447* 0.820223* 0.776418* 0.747038* 0.575423* 0.803155* 0.762886* 0.811806* 0.589449*
Peroxisomes 0.000483 0.002082 0.001888 0.001045 0.000530 0.000697 0.001464 0.000401 0.000506 0.001144 0.001649 0.000545
Vacuole 0.000959 0.002855 0.013562 0.021164 0.001565 0.002506 0.096424 0.009905 0.001256 0.002618 0.060282 0.012411
Vacuole Periphery 0.000360 0.000853 0.000375 0.002884 0.000509 0.000923 0.000702 0.004583 0.000433 0.000901 0.000559 0.004205

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -11.76 -9.71 -11.65 1.76 4.02 -1.11 -9.67 -7.06 -4.04 8.81 -10.00 -13.18 -13.82 0.63 10.56
Bud Neck -7.35 -6.92 -12.51 -7.46 2.27 -3.39 -0.21 -20.55 -17.47 -10.16 -9.29 -5.91 -27.25 -19.54 -4.52
Bud Site -5.12 -5.89 -10.43 -5.01 0.44 -4.75 -5.96 -12.53 -7.75 2.37 -6.92 -8.34 -16.23 -8.01 2.93
Cell Periphery -5.73 -6.53 -13.46 -2.73 2.64 -0.71 -9.45 -11.73 -10.03 7.51 -4.95 -10.65 -16.05 -4.68 7.70
Cytoplasm 3.58 16.56 -0.88 -3.94 -13.67 -1.32 -2.73 -11.96 -9.83 -0.05 5.51 2.80 -2.30 -8.29 -3.92
Cytoplasmic Foci 0.86 -0.77 1.89 0.99 1.74 0.58 -2.07 2.03 2.22 3.21 1.67 -1.86 3.93 2.93 3.92
Eisosomes -8.69 -8.31 -11.08 -3.11 6.23 -1.60 -10.14 -2.24 -0.47 9.84 -6.91 -12.93 -9.07 -1.19 11.64
Endoplasmic Reticulum -11.72 -4.76 -12.30 -1.63 -4.49 2.28 -7.01 -0.28 -2.85 7.00 -6.44 -7.27 -9.62 -1.61 5.97
Endosome -1.71 1.77 -2.77 -2.30 -3.17 -2.34 -4.93 -1.38 1.80 4.62 -2.76 -3.89 -3.16 -0.05 1.08
Golgi -4.25 -2.43 -0.92 -0.14 -0.62 -0.07 -2.08 -0.77 -0.82 0.50 -2.01 -2.98 -1.14 -0.47 -0.04
Lipid Particles -3.83 -3.42 -8.11 -5.36 -3.74 -0.38 -6.79 -2.58 -2.25 6.34 -2.32 -7.36 -7.72 -4.59 4.87
Mitochondria -8.74 -13.29 -6.67 -4.68 1.90 -3.49 -6.32 -22.96 -2.34 -10.55 -4.25 -12.40 -22.33 -4.02 -4.07
Mitotic Spindle -6.14 -1.60 -13.16 -10.01 -6.91 -5.45 -2.82 -24.61* -21.06 -12.74 -8.46 -3.06 -28.30 -23.17 -14.15
None 6.11 17.63 7.59 0.82 -11.39 -2.39 1.52 -17.97 -10.99 -10.62 7.43 14.74 2.85 -6.66 -17.05
Nuclear Periphery -1.46 -4.96 -14.95 -14.61 -7.91 -2.09 -6.23 -16.66 -15.64 -11.05 -2.61 -7.87 -19.95 -18.97 -12.87
Nuclear Periphery Foci -3.94 6.79 1.27 4.70 -4.32 2.08 -1.38 4.01 2.37 3.04 -1.01 1.22 5.22 6.00 1.74
Nucleolus -4.32 6.97 24.63* 22.79* 2.81 -4.12 9.11 20.96 23.44* 0.14 -6.65 11.30 28.09 30.27* 0.97
Nucleus 6.06 -1.27 10.74 5.51 6.69 7.18 2.98 19.96 12.07 3.43 8.18 1.48 21.48 12.81 6.67
Peroxisomes -5.37 -3.86 -0.25 5.18 3.80 -1.93 -4.57 2.69 4.67 5.03 -5.26 -5.95 2.67 7.25 6.40
Vacuole -4.89 -4.03 -11.03 -10.50 -4.25 -2.53 -5.23 -10.98 -8.58 4.23 -4.18 -6.01 -15.54 -13.41 3.32
Vacuole Periphery -2.29 -2.74 -1.02 0.28 -0.38 -1.64 -1.72 -0.83 0.23 -0.05 -2.70 -2.89 -1.27 0.30 -0.30
Standard name
Human Ortholog
Description Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mit1

Mit1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mit1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available