Standard name
Human Ortholog
Description Subunit of the heterohexameric cochaperone prefoldin complex; complex binds specifically to cytosolic chaperonin and transfers target proteins to it
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.2 – 0.21 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.97 0.96 0.87 – 0.73 0.69 0.67 0.66 0.63 0.98 0.99 0.99 0.83 – 0.91 0.97 0.97 0.92 0.94 0.91 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.17 0.08 0.32 – 0.48 0.59 0.53 0.61 0.55 0 0 0 0 – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0 0.22 – 0.19 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 1 – 0 0 0 2 1 1 1 1 0 – 0 2 0 2 0 0 1
Bud 3 1 12 2 2 4 – 13 4 7 12 7 2 2 1 0 – 0 2 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 13 8 4 6 7 5 – 9 10 7 15 19 34 21 48 29 – 22 0 0 0 0 0 0
Cytoplasm 810 198 310 232 365 377 – 335 325 318 255 291 795 574 917 123 – 95 808 197 293 488 459 489
Endoplasmic Reticulum 3 2 4 1 4 1 – 1 2 10 4 24 12 3 2 4 – 3 0 0 5 10 12 8
Endosome 0 0 0 0 0 0 – 2 1 0 1 0 0 0 2 2 – 0 3 2 0 2 3 3
Golgi 1 1 0 0 0 0 – 0 0 0 0 0 1 0 0 0 – 1 3 1 1 3 5 3
Mitochondria 6 1 8 40 30 138 – 220 279 252 234 252 2 0 2 0 – 0 2 0 7 2 3 2
Nucleus 4 1 0 0 0 1 – 1 0 5 0 1 1 0 6 2 – 0 0 0 0 0 1 0
Nuclear Periphery 0 1 0 0 0 0 – 2 4 2 1 0 1 0 0 0 – 0 1 0 1 0 1 0
Nucleolus 1 0 0 0 0 1 – 3 0 0 2 1 0 0 0 0 – 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 1 1
Vac/Vac Membrane 2 0 0 0 2 1 – 23 25 9 4 4 5 1 2 32 – 20 4 0 2 5 6 2
Unique Cell Count 821 203 314 240 379 433 462 469 472 386 462 815 580 926 148 104 834 204 321 517 503 521
Labelled Cell Count 843 213 338 281 412 529 609 650 610 530 600 854 603 981 192 141 834 204 321 517 503 521


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 8.7 5.2 6.2 6.2 5.2 – 5.4 4.6 5.0 4.7 4.7 8.3 8.4 8.3 14.7 – 13.6 8.7 8.9 8.8
Std Deviation (1e-4) 1.0 1.1 0.9 1.4 1.1 1.0 – 1.4 1.0 0.9 0.9 0.8 1.1 1.2 1.1 10.5 – 2.7 1.3 1.4 1.4
Intensity Change (Log2) – – – 0.26 0.26 0.01 – 0.05 -0.17 -0.06 -0.13 -0.13 0.68 0.7 0.68 1.5 – 1.39 0.74 0.78 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud -2.2 -3.1 -2.7 – -0.8 0 -2.1 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 – 0.7 0 0 0 0 2.4 2.0 3.0 7.1 – 7.3
Cytoplasm -1.7 -2.0 -5.8 – -9.6 -10.3 -10.7 -10.9 -11.7 -1.2 0.3 0.5 -6.5 – -3.8
Endoplasmic Reticulum 0 0 0 – 0 0 0.9 0 0 0 0 0 0 – 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Mitochondria 5.9 3.1 10.0 – 13.5 0 14.8 0 0 0 0 0 0 – 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 – 4.0 4.2 0 0 0 0 0 0 8.5 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1529 6.5855 6.1828 6.0758 5.3744 6.3162 4.2105 5.4328 4.6243 4.5317 4.5029 4.8847 6.1518 7.4428 6.7669 6.6511 6.6944 6.834
Actin 0.0053 0.0001 0.0112 0.0204 0.0094 0.002 0.0095 0.0006 0.0096 0.0327 0.0113 0.0017 0.0004 0.0002 0.0002 0.0002 0.0002 0.0001
Bud 0.0002 0.0001 0.0035 0.0015 0.001 0.0004 0.0018 0.0021 0.0012 0.0027 0.0004 0.0003 0.0001 0.0001 0.0003 0.0003 0.0001 0.0001
Bud Neck 0.0003 0.0001 0.0005 0.0009 0.0018 0.0013 0.0038 0.0002 0.001 0.0021 0.0016 0.0021 0.0001 0.0001 0.0003 0.0003 0.0002 0.0004
Bud Periphery 0.0003 0 0.0015 0.0014 0.001 0.0006 0.0019 0.0012 0.0006 0.0039 0.0007 0.0004 0 0 0.0001 0.0001 0 0.0001
Bud Site 0.0003 0.0004 0.0022 0.0019 0.0016 0.0011 0.0049 0.0043 0.0101 0.0017 0.0011 0.0003 0.0001 0.0012 0.0004 0.0002 0.0001 0
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.9031 0.9599 0.9057 0.8314 0.7738 0.948 0.8462 0.9419 0.8881 0.8222 0.8338 0.9393 0.9235 0.9756 0.9589 0.9222 0.9067 0.961
Cytoplasmic Foci 0.0231 0.0061 0.012 0.0289 0.0276 0.0111 0.0224 0.013 0.0212 0.0238 0.021 0.0133 0.0052 0.0047 0.0067 0.0129 0.0272 0.0086
Eisosomes 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0059 0.0046 0.0047 0.0038 0.007 0.006 0.0118 0.0019 0.0084 0.0034 0.0054 0.0042 0.0187 0.0029 0.0048 0.0094 0.0026 0.0051
Endosome 0.0224 0.0079 0.03 0.0452 0.0733 0.0148 0.0436 0.0103 0.0212 0.0438 0.0632 0.0173 0.0064 0.0037 0.0135 0.0381 0.0339 0.0121
Golgi 0.0028 0.0005 0.0037 0.0048 0.0131 0.0007 0.0076 0.0024 0.0021 0.0076 0.0054 0.0033 0.0005 0.0005 0.0006 0.0007 0.0009 0.0006
Lipid Particles 0.0021 0 0.0009 0.0031 0.0078 0.0001 0.0017 0.0001 0.0031 0.0159 0.0054 0.0009 0.0001 0.0001 0 0.0001 0.0002 0
Mitochondria 0.0032 0.0002 0.0045 0.0045 0.0108 0.0017 0.0061 0.0009 0.0007 0.0065 0.0226 0.0024 0.0002 0.0002 0.0004 0.0002 0.0003 0.0013
None 0.018 0.0159 0.0065 0.0127 0.0056 0.0043 0.021 0.0161 0.0214 0.0087 0.0048 0.0092 0.0397 0.0082 0.0091 0.0044 0.0196 0.008
Nuclear Periphery 0.0033 0.0005 0.0024 0.0056 0.0302 0.0027 0.0049 0.0004 0.001 0.0016 0.0041 0.0006 0.001 0.0003 0.0006 0.0014 0.0007 0.0005
Nucleolus 0.0001 0 0.0001 0.0004 0.0014 0.0003 0.0005 0 0.0001 0.0001 0.0007 0 0 0 0 0 0 0
Nucleus 0.0011 0.0009 0.0018 0.0033 0.0143 0.0018 0.0033 0.0009 0.0014 0.0016 0.002 0.0012 0.0008 0.0005 0.0009 0.0013 0.0009 0.0007
Peroxisomes 0.0038 0 0.0011 0.0164 0.0041 0.0006 0.0016 0.0003 0.0035 0.0084 0.0031 0.0005 0 0 0 0 0.0001 0
Punctate Nuclear 0.0015 0.0001 0.0012 0.0031 0.005 0.0004 0.0018 0.0005 0.001 0.0033 0.0051 0.0004 0.0001 0.0001 0.0001 0.0002 0.0004 0.0001
Vacuole 0.0025 0.002 0.0053 0.0082 0.0072 0.0016 0.0041 0.0023 0.0034 0.0076 0.0061 0.0018 0.0026 0.0012 0.0026 0.0066 0.0052 0.0011
Vacuole Periphery 0.0007 0.0003 0.0009 0.0022 0.0037 0.0005 0.0013 0.0003 0.0006 0.0022 0.002 0.0005 0.0003 0.0002 0.0005 0.0012 0.0007 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.3332 64.5842 52.281 59.3017 48.9644 85.3352 54.1701 64.1073 50.7181 64.424
Translational Efficiency 3.594 2.1967 2.1583 2.0609 3.0079 1.592 1.8767 1.7752 2.0033 2.1711

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1864 1353 352 1650 1396 2040 1446 1194 3260 3393 1798 2844

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 778.30 893.25 1018.61 1070.27 822.04 923.90 958.74 1034.70 797.03 911.68 970.46 1055.34
Standard Deviation 106.44 129.67 152.99 152.49 112.03 117.01 138.80 147.49 111.00 123.13 143.67 151.43
Intensity Change Log 2 — 0.198738 0.388204 0.459577 — 0.168528 0.221931 0.331932 — 0.183299 0.305190 0.395421

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000269 0.000307 0.000464 0.001003 0.000261 0.000152 0.000465 0.000678 0.000265 0.000214 0.000465 0.000866
Bud Neck 0.008742 0.004086 0.001463 0.002002 0.004778 0.004340 0.001509 0.002691 0.007045 0.004239 0.001500 0.002291
Bud Site 0.021423 0.026893 0.008358 0.034100 0.014806 0.017456 0.019861 0.034881 0.018589 0.021219 0.017609 0.034428
Cell Periphery 0.000225 0.000108 0.000129 0.000089 0.000126 0.000084 0.000081 0.000068 0.000182 0.000094 0.000091 0.000080
Cytoplasm 0.508026* 0.625158* 0.654543* 0.738317* 0.587346* 0.672338* 0.825611* 0.714664* 0.541992* 0.653525* 0.792120* 0.728387*
Cytoplasmic Foci 0.309052* 0.211932* 0.063054 0.049302 0.236950* 0.192439* 0.055661 0.057915 0.278177* 0.200212* 0.057108 0.052918
Eisosomes 0.000158 0.000037 0.000036 0.000015 0.000068 0.000035 0.000008 0.000013 0.000120 0.000036 0.000014 0.000014
Endoplasmic Reticulum 0.003788 0.001777 0.001560 0.000763 0.003220 0.001148 0.000382 0.000232 0.003545 0.001399 0.000613 0.000540
Endosome 0.028074 0.026347 0.010753 0.005730 0.023221 0.016582 0.004266 0.006908 0.025996 0.020476 0.005536 0.006224
Golgi 0.022012 0.009977 0.000337 0.003401 0.010940 0.009071 0.002533 0.003029 0.017271 0.009432 0.002103 0.003245
Lipid Particles 0.004955 0.001731 0.002088 0.000978 0.003300 0.001383 0.000683 0.000661 0.004246 0.001521 0.000958 0.000845
Mitochondria 0.006798 0.001438 0.000078 0.003860 0.003130 0.001449 0.001298 0.001774 0.005227 0.001445 0.001059 0.002984
Mitotic Spindle 0.001190 0.003106 0.003562 0.023137 0.001127 0.002614 0.004758 0.022313 0.001163 0.002810 0.004524 0.022791
None 0.001020 0.000938 0.001457 0.000590 0.001099 0.000953 0.001181 0.000674 0.001054 0.000947 0.001235 0.000625
Nuclear Periphery 0.000150 0.000056 0.000434 0.000122 0.000144 0.000028 0.000054 0.000132 0.000148 0.000039 0.000128 0.000126
Nuclear Periphery Foci 0.000335 0.000883 0.009514 0.002157 0.000404 0.000293 0.001747 0.001530 0.000365 0.000529 0.003268 0.001894
Nucleolus 0.000235 0.000233 0.000232 0.000249 0.000266 0.000222 0.000173 0.000241 0.000248 0.000226 0.000184 0.000246
Nucleus 0.010949 0.006436 0.047301 0.013680 0.012015 0.009403 0.014947 0.012081 0.011405 0.008220 0.021281 0.013009
Peroxisomes 0.002555 0.002956 0.000370 0.001819 0.001617 0.001750 0.000559 0.002176 0.002153 0.002231 0.000522 0.001969
Vacuole 0.062475 0.072166 0.193696 0.117422 0.090401 0.065368 0.063617 0.136072 0.074433 0.068079 0.089083 0.125252
Vacuole Periphery 0.007569 0.003436 0.000573 0.001264 0.004780 0.002892 0.000607 0.001268 0.006375 0.003109 0.000600 0.001266

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.14 -1.61 -3.24 -2.89 -0.53 2.04 -1.17 -1.34 -2.51 0.18 1.83 -1.53 -3.31 -4.20 -0.34
Bud Neck 7.81 14.81 14.73 5.95 -0.61 1.07 8.53 6.39 6.30 -1.85 7.64 16.34 15.79 9.54 -1.60
Bud Site -3.17 6.22 0.26 2.63 -4.79 -1.79 -1.32 -3.12 -2.15 -1.74 -2.32 1.57 -1.93 -0.35 -2.76
Cell Periphery 7.86 6.19 11.10 4.96 6.17 3.44 2.63 5.86 6.09 1.55 9.14 6.48 12.44 7.09 2.05
Cytoplasm -12.51 -7.32 -21.06 -6.48 -4.27 -9.73 -23.71 -8.94 -0.86 12.21 -17.82 -31.01 -21.49 -4.77 10.51
Cytoplasmic Foci 14.31 43.49 54.65 30.92 3.54 6.86 32.47 32.63 29.61 0.95 16.73 56.09 61.77 43.63 3.40
Eisosomes 12.61 13.77 17.23 4.85 8.93 6.66 12.91 12.17 11.56 -3.99 14.75 19.86 20.31 10.09 1.98
Endoplasmic Reticulum 2.76 3.69 5.79 2.78 6.51 2.76 4.96 5.35 1.95 1.20 4.03 7.06 7.82 2.99 2.06
Endosome 0.98 13.64 22.02 14.45 6.64 4.75 16.05 14.79 12.03 -1.22 5.06 24.53 26.36 19.22 1.96
Golgi 9.38 28.33 22.99 7.40 -2.60 2.02 8.96 9.30 8.39 0.36 10.25 20.23 23.36 11.80 0.09
Lipid Particles 8.53 7.21 11.25 4.72 4.59 7.69 10.12 10.99 6.06 1.76 11.49 13.53 14.97 7.10 2.51
Mitochondria 5.42 7.19 4.33 -0.28 -2.14 2.14 2.32 2.50 0.86 0.05 5.74 5.67 4.97 0.17 -0.82
Mitotic Spindle -1.82 -1.03 -6.22 -5.08 -3.69 -1.99 -2.97 -5.42 -4.68 -3.77 -2.62 -3.07 -8.24 -7.00 -5.59
None 0.27 -0.75 1.50 1.23 2.95 0.31 0.13 1.01 2.38 0.99 0.42 -0.03 1.68 2.60 1.34
Nuclear Periphery 1.60 -5.42 -0.98 -9.31 7.91 2.25 1.12 -0.37 -5.95 -3.32 2.71 -0.59 -1.02 -11.66 -1.17
Nuclear Periphery Foci -2.26 -11.31 -5.23 -2.36 9.04 2.33 -9.92 -8.43 -9.52 2.09 -1.66 -14.20 -7.63 -5.77 6.42
Nucleolus -0.07 0.20 0.60 0.74 0.58 0.72 1.31 0.85 0.42 -0.55 0.44 1.28 1.00 0.85 -0.38
Nucleus 5.38 -14.20 -9.00 -17.02 10.75 3.42 -6.67 -4.77 -8.16 1.96 5.31 -13.35 -10.18 -17.11 5.78
Peroxisomes -0.87 7.97 4.98 4.73 -4.06 -0.49 6.49 0.98 1.33 -2.43 -0.26 9.43 4.46 4.79 -3.85
Vacuole -3.09 -13.13 -23.37 -20.36 2.38 6.88 0.09 -15.19 -20.05 -14.80 2.45 -9.77 -27.41 -29.26 -13.90
Vacuole Periphery 8.07 15.71 15.18 9.73 -0.73 3.89 10.06 8.94 6.86 -0.95 9.08 18.33 17.48 11.47 -1.12
Standard name
Human Ortholog
Description Subunit of the heterohexameric cochaperone prefoldin complex; complex binds specifically to cytosolic chaperonin and transfers target proteins to it
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Gim4

Gim4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gim4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available