Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0 0 0 0 0.08 0.1 0.11 0.06 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.11 0 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0 0.16 0.1 0.13 0.13 0.13 0.08 0 0.12 0.09 0.14 0 0 0 0.08 0.05 0.05
Nucleus 0.37 0.53 0.41 0 0.12 0.14 0.08 0.14 0.15 0.18 0.17 0 0.06 0.05 0.17 0.17 0.1 0.13 0.32 0.1 0.05 0 0.05
Nuclear Periphery 0.08 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0.09 0 0.05 0.05
Nucleolus 0.51 0.44 0.55 0.96 0.82 0.81 0.8 0.71 0.75 0.69 0.7 0.89 0.82 0.81 0.69 0.78 0.76 0.66 0.6 0.71 0.62 0.53 0.51
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.21 0.17 0.19 0.28 0.16 0.18 0.14 0.15 0 0.07 0.09 0 0 0 0 0 0 0.1 0.16 0.11
Vac/Vac Membrane 0.1 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0 0 0.07 0 0 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 3 1 0 0 0 1 0 0 0 3 0 1 2 5 6
Bud Neck 0 0 0 3 0 1 1 0 2 0 1 0 8 5 0 0 0 0 0 0 0 2 3
Bud Site 1 0 0 0 0 5 1 10 10 6 10 0 0 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2 0 2 3 7 15 6 5 8 3 24 44 58 7 3 5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Endosome 1 0 0 2 6 0 32 3 15 10 21 3 12 12 1 1 0 4 0 0 6 26 37
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3
Mitochondria 0 2 1 2 2 19 10 33 27 31 37 42 36 21 14 8 10 2 0 2 19 25 24
Nucleus 72 61 44 2 16 22 25 28 39 41 49 12 29 25 19 15 7 30 42 14 12 20 23
Nuclear Periphery 16 2 6 0 3 2 4 6 3 6 7 0 0 0 0 1 0 28 1 12 7 26 22
Nucleolus 100 51 59 108 113 128 235 146 196 160 200 279 376 424 79 69 53 156 80 97 148 270 227
Peroxisomes 1 0 0 1 1 0 10 0 5 1 2 7 9 19 1 0 0 0 0 0 2 5 5
SpindlePole 1 0 0 23 23 30 82 33 48 32 44 13 31 47 0 1 0 3 0 1 23 82 48
Vac/Vac Membrane 19 7 6 0 5 0 4 2 4 3 6 1 4 4 13 6 3 7 8 6 10 28 31
Unique Cell Count 196 116 107 112 138 158 295 205 262 232 286 313 461 526 114 88 70 237 133 138 241 507 448
Labelled Cell Count 211 125 116 143 172 214 419 267 357 299 380 381 550 617 135 104 78 237 133 138 241 507 448


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.2 19.0 17.8 15.8 15.1 8.3 11.3 8.4 9.0 8.3 8.6 21.1 20.0 18.6 26.1 29.4 29.1 18.9 18.8 18.9
Std Deviation (1e-4) 5.7 5.0 3.9 5.1 4.2 2.8 3.3 2.3 2.8 2.6 2.5 7.9 9.2 8.9 13.4 11.7 13.0 4.4 6.3 6.2
Intensity Change (Log2) -0.17 -0.23 -1.11 -0.65 -1.08 -0.98 -1.09 -1.06 0.24 0.17 0.07 0.56 0.73 0.71 0.08 0.08 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 2.9 3.3 3.6 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 3.6 0 0 0 2.9 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 0 4.1 3.1 3.6 3.6 3.7 2.6 0 3.4 0 0
Nucleus -7.1 -5.3 -5.0 -7.7 -5.5 -5.5 -4.6 -5.0 -9.8 -9.7 -11.0 -4.0 -3.6 -4.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 7.2 4.5 4.5 4.9 2.8 3.7 2.5 2.8 7.7 5.8 5.6 2.2 3.4 2.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 5.0 4.4 4.8 6.1 4.4 4.7 4.0 4.3 0 2.8 3.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 1.5 0.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.0427 14.9742 13.1755 9.8826 11.0285 13.2945 16.36 18.614 17.1871 15.6336 14.9452 16.9393 17.4431 20.8607 17.8169 16.5256 16.8125 18.6942
Actin 0.0149 0.0013 0.0005 0.0058 0.0184 0.0015 0.0017 0.0002 0.0171 0.0003 0.0411 0.0001 0.0106 0 0.0054 0 0.0211 0.0002
Bud 0.0003 0 0.0001 0.001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0005 0 0.0002 0 0.0002 0 0.0009 0
Bud Neck 0.0002 0.0002 0.0002 0.0004 0.0012 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Periphery 0.0006 0.0002 0.0003 0.0012 0.0008 0.0004 0.0002 0.0001 0.0005 0.0001 0.0007 0.0002 0.0004 0 0.0002 0.0001 0.0024 0.0002
Bud Site 0.0007 0.0002 0.0013 0.0024 0.0018 0.0001 0.0007 0.0001 0.0012 0.0002 0.0011 0.0001 0.0066 0.0001 0.0008 0.0001 0.0006 0.0001
Cell Periphery 0.0005 0.0007 0.0006 0.001 0.0013 0.0015 0.0005 0.0002 0.0002 0.0002 0.0003 0.0005 0.0006 0.0002 0.0002 0.0002 0.0006 0.0004
Cytoplasm 0.0009 0 0.0001 0.0003 0.0003 0 0.0079 0.0001 0.0007 0.0002 0.0014 0.0001 0.0015 0 0.0003 0 0.0004 0
Cytoplasmic Foci 0.006 0.0025 0.0041 0.0052 0.0062 0.0041 0.0058 0.0019 0.001 0.0049 0.0098 0.0033 0.0072 0.0005 0.0034 0.0012 0.0049 0.0014
Eisosomes 0.0002 0 0.0001 0.0002 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.0016 0 0.0001 0.0007 0.0006 0.0002 0.0006 0 0.0011 0.0001 0.0019 0.0001 0.0008 0 0.0001 0 0.0018 0.0001
Endosome 0.0197 0.0005 0.0035 0.0251 0.012 0.002 0.0081 0.0032 0.0084 0.0214 0.0288 0.0005 0.0073 0.0001 0.0002 0 0.0035 0.0001
Golgi 0.0076 0.0014 0.003 0.022 0.0136 0.0019 0.0008 0.0021 0.0025 0.0042 0.0065 0.0007 0.0025 0.0001 0.0003 0 0.0028 0.0004
Lipid Particles 0.0281 0.0055 0.0305 0.0491 0.0669 0.0632 0.0121 0.0015 0.0034 0.0064 0.012 0.0506 0.0163 0.0034 0.0069 0.0026 0.0128 0.0284
Mitochondria 0.0306 0.0027 0.0095 0.066 0.0169 0.0799 0.0136 0.0012 0.0066 0.0355 0.0133 0.0064 0.0119 0.0002 0.0075 0.0012 0.0143 0.021
None 0.001 0 0.0001 0.0006 0.0007 0.0002 0.0113 0.0001 0.0003 0.0003 0.0004 0.0002 0.0011 0 0.0006 0 0.0006 0.0001
Nuclear Periphery 0.0434 0.0177 0.0203 0.0041 0.1037 0.0499 0.0177 0.0165 0.0104 0.0557 0.038 0.0851 0.0079 0.0201 0.0131 0.0392 0.0315 0.042
Nucleolus 0.7626 0.8437 0.8454 0.719 0.5717 0.6579 0.7979 0.8512 0.8684 0.8081 0.6869 0.7358 0.8259 0.8474 0.8677 0.8397 0.7209 0.7961
Nucleus 0.0554 0.1013 0.0664 0.0536 0.1511 0.0723 0.0851 0.1171 0.0686 0.051 0.0972 0.0749 0.0784 0.1235 0.0822 0.1112 0.1423 0.0891
Peroxisomes 0.0028 0.0042 0.0018 0.0023 0.0077 0.0039 0.0009 0.0002 0.0002 0.0009 0.0022 0.0008 0.0026 0 0.0004 0.0001 0.0082 0.0003
Punctate Nuclear 0.0037 0.0014 0.0028 0.0138 0.0077 0.0056 0.02 0.0017 0.0008 0.0032 0.0522 0.0079 0.0118 0.0016 0.005 0.0009 0.0208 0.0066
Vacuole 0.004 0.0024 0.0023 0.0038 0.0063 0.0047 0.0021 0.0004 0.0022 0.0013 0.0015 0.0016 0.0017 0.0004 0.001 0.0006 0.0014 0.0009
Vacuole Periphery 0.0153 0.014 0.0069 0.0225 0.0107 0.0501 0.0125 0.002 0.0058 0.0056 0.0036 0.0308 0.0043 0.0023 0.0043 0.0028 0.0079 0.0123

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.3656 26.9269 33.6521 38.0288 58.5811 39.6118 18.6282 17.892 29.533 30.2584
Translational Efficiency 10.2875 9.3326 5.6821 6.7838 4.1685 4.0215 7.389 6.4137 4.3844 5.5952

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1555 1111 149 1555 1635 1870 2433 2073 3190 2981 2582 3628

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1453.90 1454.39 1774.26 2065.62 1383.11 1611.32 1907.02 2103.65 1417.62 1552.83 1899.36 2087.35
Standard Deviation 311.45 407.66 322.06 649.72 276.44 338.38 403.27 530.20 296.15 373.53 400.24 584.73
Intensity Change Log 2 0.000486 0.287289 0.506647 0.220327 0.463404 0.604979 0.111850 0.375836 0.555424

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000799 0.000816 0.000574 0.001461 0.000552 0.000655 0.000909 0.000928 0.000672 0.000715 0.000890 0.001156
Bud Neck 0.000828 0.002054 0.001871 0.007852 0.000958 0.001494 0.001830 0.008380 0.000895 0.001702 0.001832 0.008154
Bud Site 0.000136 0.000648 0.000239 0.001513 0.000210 0.000216 0.000152 0.002082 0.000174 0.000377 0.000157 0.001838
Cell Periphery 0.000015 0.000103 0.000012 0.000322 0.000021 0.000028 0.000025 0.000117 0.000018 0.000056 0.000025 0.000205
Cytoplasm 0.000533 0.001629 0.000013 0.000778 0.000306 0.000054 0.000042 0.001037 0.000416 0.000641 0.000040 0.000926
Cytoplasmic Foci 0.000963 0.004814 0.001058 0.009432 0.002050 0.001157 0.000427 0.004045 0.001521 0.002520 0.000463 0.006354
Eisosomes 0.000119 0.000181 0.000079 0.000285 0.000182 0.000204 0.000172 0.000175 0.000151 0.000196 0.000167 0.000222
Endoplasmic Reticulum 0.000381 0.000677 0.000233 0.000560 0.000420 0.000301 0.000200 0.000248 0.000401 0.000441 0.000202 0.000382
Endosome 0.000029 0.000130 0.000027 0.000447 0.000125 0.000135 0.000019 0.000315 0.000079 0.000133 0.000019 0.000372
Golgi 0.000076 0.000463 0.000210 0.000839 0.000168 0.000108 0.000076 0.000339 0.000123 0.000240 0.000084 0.000554
Lipid Particles 0.000030 0.000180 0.000043 0.000978 0.000317 0.000134 0.000014 0.000146 0.000177 0.000151 0.000016 0.000502
Mitochondria 0.001476 0.003228 0.002023 0.010675 0.002354 0.001943 0.002368 0.005674 0.001926 0.002422 0.002348 0.007818
Mitotic Spindle 0.000916 0.001562 0.005455 0.003426 0.001778 0.001178 0.000470 0.006850 0.001358 0.001321 0.000757 0.005383
None 0.000372 0.002374 0.000318 0.002118 0.000589 0.000568 0.000415 0.000876 0.000483 0.001241 0.000409 0.001408
Nuclear Periphery 0.013561 0.003887 0.005724 0.018178 0.006714 0.006182 0.008413 0.013495 0.010051 0.005326 0.008258 0.015502
Nuclear Periphery Foci 0.004785 0.003988 0.001792 0.010811 0.007266 0.003735 0.002199 0.004892 0.006056 0.003829 0.002176 0.007429
Nucleolus 0.720999* 0.682239* 0.764968* 0.669515* 0.766188* 0.701082* 0.671741* 0.659547* 0.744160* 0.694059* 0.677121* 0.663819*
Nucleus 0.234418* 0.246754* 0.176293* 0.185248* 0.171139* 0.254214* 0.286764* 0.248812* 0.201985* 0.251434* 0.280389* 0.221568*
Peroxisomes 0.012169 0.035770 0.037079 0.027179 0.035111 0.021959 0.013194 0.020079 0.023927 0.027106 0.014573 0.023122
Vacuole 0.000978 0.002191 0.000576 0.012478 0.001071 0.001053 0.001079 0.003493 0.001026 0.001477 0.001050 0.007344
Vacuole Periphery 0.006417 0.006314 0.001413 0.035903 0.002479 0.003598 0.009491 0.018470 0.004399 0.004611 0.009025 0.025942

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.40 3.66 -6.17 -5.49 -7.56 -1.33 -4.99 -5.29 -6.06 -0.97 -0.92 -4.89 -8.12 -7.95 -5.09
Bud Neck -4.11 -4.13 -15.20 -11.09 -11.92 -3.81 -6.15 -14.41 -13.36 -12.58 -5.62 -7.01 -20.16 -17.48 -16.98
Bud Site -2.78 -1.38 -9.28 -3.50 -8.08 -0.13 0.88 -4.30 -4.31 -4.44 -2.59 0.62 -6.13 -5.33 -6.25
Cell Periphery -2.37 1.77 -3.24 -2.17 -3.28 -1.28 -0.93 -6.19 -5.81 -6.07 -2.67 -2.06 -4.62 -3.55 -4.48
Cytoplasm -1.19 1.02 -0.33 1.17 -3.70 1.06 1.11 -1.70 -2.64 -2.67 -0.58 1.34 -1.32 -0.67 -3.83
Cytoplasmic Foci -2.91 0.31 -7.85 -3.45 -9.48 1.78 4.25 -4.25 -6.19 -8.47 -1.65 3.31 -8.69 -6.64 -13.54
Eisosomes -4.32 4.16 -7.14 -4.06 -8.92 -1.63 0.84 0.65 2.14 -0.22 -4.47 -1.55 -5.93 -1.92 -4.04
Endoplasmic Reticulum -2.20 2.49 -1.23 0.58 -2.12 1.07 1.94 1.37 1.08 -3.61 -0.43 3.25 0.13 0.57 -2.99
Endosome -4.20 0.40 -4.41 -3.28 -4.44 -0.13 1.64 -1.71 -1.64 -3.05 -1.08 1.78 -3.87 -3.06 -5.08
Golgi -1.81 -4.52 -5.89 -1.56 -4.76 1.51 2.41 -4.03 -8.66 -10.92 -1.40 1.98 -7.20 -3.32 -8.28
Lipid Particles -1.66 -0.55 -2.73 -2.20 -2.69 1.12 1.97 1.04 -0.29 -4.48 0.24 2.04 -1.95 -2.24 -3.27
Mitochondria -4.94 -1.80 -10.93 -8.27 -9.80 0.89 -0.05 -6.26 -9.33 -9.02 -1.70 -1.69 -12.74 -12.06 -12.71
Mitotic Spindle -0.84 -1.48 -2.80 -2.24 0.64 1.06 3.37 -5.10 -5.56 -6.60 0.10 1.62 -5.83 -5.96 -7.07
None -4.33 1.38 -9.52 0.55 -9.84 0.12 0.91 -1.48 -2.11 -5.13 -3.49 0.70 -6.95 -0.82 -9.96
Nuclear Periphery 5.57 2.37 -1.84 -7.01 -3.55 0.37 -1.22 -3.75 -4.09 -2.85 4.11 1.41 -3.58 -7.52 -4.87
Nuclear Periphery Foci 0.81 3.85 -5.02 -5.34 -7.83 3.24 5.36 2.41 -1.37 -4.93 3.07 6.55 -1.71 -5.06 -9.20
Nucleolus 4.06 -2.52 5.79 1.35 5.54 8.91 12.84 14.56 5.90 2.28 8.52 10.17 14.19 5.46 2.50
Nucleus -1.35 3.40 6.02 6.67 -0.55 -11.81 -16.30 -11.30 0.15 4.28 -8.67 -12.13 -4.05 4.56 8.40
Peroxisomes -12.04 -5.55 -9.90 3.53 2.20 7.66 13.94 8.48 1.16 -7.19 -2.61 9.14 0.36 3.03 -9.04
Vacuole -4.55 2.68 -10.67 -9.44 -11.09 0.06 -0.07 -7.86 -7.23 -8.43 -2.42 -0.19 -12.67 -11.49 -12.69
Vacuole Periphery 0.08 4.74 -11.75 -12.03 -15.10 -2.08 -8.19 -10.06 -9.17 -5.01 -0.31 -5.02 -15.02 -15.23 -11.02
Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis
Localization
Cell Percentages nucleolus (37%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Nop16

Nop16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available