Standard name | |
---|---|
Human Ortholog | |
Description | Apyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 14 | 3 | 5 | – | 2 | 0 | 0 | 3 | 3 | 0 | 1 | 2 |
Bud | 0 | 4 | 0 | 3 | 4 | 4 | 13 | 13 | 21 | 20 | 17 | 18 | 3 | 1 | 2 | – | 1 | 0 | 1 | 2 | 3 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 1 | 2 | – | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 27 | 228 | 232 | 179 | 243 | 283 | 345 | 332 | 284 | 372 | 283 | 364 | 204 | 360 | 335 | – | 348 | 157 | 6 | 16 | 9 | 3 | 15 | 17 |
Endoplasmic Reticulum | 1 | 17 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 3 | 5 | – | 25 | 16 | 0 | 3 | 9 | 0 | 5 | 6 |
Endosome | 0 | 26 | 62 | 34 | 31 | 3 | 8 | 7 | 3 | 5 | 1 | 3 | 9 | 18 | 20 | – | 28 | 26 | 14 | 102 | 100 | 20 | 21 | 22 |
Golgi | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 1 | 15 | 12 | 2 | 4 | 6 |
Mitochondria | 9 | 38 | 11 | 13 | 27 | 115 | 150 | 127 | 149 | 186 | 155 | 155 | 0 | 0 | 1 | – | 20 | 5 | 10 | 46 | 41 | 23 | 34 | 27 |
Nucleus | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 1 | 1 | 0 | 1 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 2 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
SpindlePole | 0 | 2 | 7 | 5 | 2 | 3 | 9 | 5 | 9 | 9 | 9 | 19 | 0 | 7 | 4 | – | 5 | 1 | 2 | 1 | 2 | 0 | 2 | 1 |
Vac/Vac Membrane | 15 | 119 | 98 | 31 | 44 | 13 | 12 | 7 | 4 | 12 | 4 | 8 | 7 | 14 | 8 | – | 78 | 63 | 49 | 250 | 195 | 33 | 100 | 79 |
Unique Cell Count | 46 | 378 | 361 | 235 | 318 | 354 | 443 | 423 | 360 | 465 | 364 | 470 | 250 | 404 | 372 | 433 | 217 | 89 | 450 | 391 | 88 | 193 | 172 | |
Labelled Cell Count | 54 | 442 | 425 | 267 | 355 | 424 | 542 | 498 | 476 | 609 | 477 | 571 | 264 | 409 | 387 | 511 | 268 | 89 | 450 | 391 | 88 | 193 | 172 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.5 | 5.2 | 4.8 | 4.7 | 3.5 | 3.3 | 3.7 | 3.1 | 3.1 | 3.1 | 3.0 | 5.8 | 5.6 | 5.7 | 4.7 | 5.4 | 5.9 | 5.7 | 6.6 | 6.9 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.4 | 1.5 | 2.0 | 1.2 | 1.1 | 1.3 | 0.8 | 1.4 | 1.1 | 0.9 | 0.7 | 0.8 | 0.8 | 0.8 | 0.9 | 1.6 | 0.8 | 1.5 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.17 | -0.56 | -0.65 | -0.49 | -0.76 | -0.76 | -0.76 | -0.81 | 0.16 | 0.09 | 0.13 | -0.14 | 0.06 | 0.18 | 0.13 | 0.34 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 3.1 | 3.4 | 4.7 | 4.3 | 4.4 | 4.4 | 5.1 | 4.0 | 4.2 | 4.7 | 8.2 | 8.3 | – | 5.1 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | -0.6 | – | 2.7 | 3.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.1 | -5.7 | -5.1 | – | -4.7 | -1.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.5 | -3.0 | – | 1.1 | -0.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | -1.0 | – | -0.9 | 0 |
Vacuole | -4.0 | -4.3 | -8.7 | -10.0 | -10.4 | -10.0 | -10.3 | -10.1 | -10.9 | -7.8 | -9.2 | -9.6 | – | -3.1 | 0.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0685 | 1.3522 | 1.393 | 1.5778 | 1.492 | 1.2089 | 2.8244 | 3.7528 | 3.7512 | 3.4004 | 3.1815 | 4.0331 | 3.045 | 3.5138 | 3.6417 | 3.5747 | 3.447 | 3.8494 |
Actin | 0.0316 | 0.0009 | 0.0208 | 0.023 | 0.032 | 0.004 | 0.0018 | 0.0031 | 0.0012 | 0.0015 | 0.0003 | 0.0012 | 0.0002 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0004 |
Bud | 0.0036 | 0.0032 | 0.0007 | 0.0004 | 0.0017 | 0.0002 | 0.0007 | 0.0012 | 0.0012 | 0.0013 | 0.0004 | 0.0004 | 0.0006 | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0002 |
Bud Neck | 0.0029 | 0.0006 | 0.0014 | 0.0018 | 0.0006 | 0.0012 | 0.0053 | 0.0004 | 0.0003 | 0.0005 | 0.0005 | 0.0008 | 0.0003 | 0.0002 | 0.0003 | 0.0074 | 0.0003 | 0.0007 |
Bud Periphery | 0.0062 | 0.0016 | 0.0008 | 0.0005 | 0.0031 | 0.0004 | 0.0008 | 0.0015 | 0.002 | 0.0038 | 0.0005 | 0.0006 | 0.0004 | 0.0007 | 0.0003 | 0.0004 | 0.0002 | 0.0003 |
Bud Site | 0.0046 | 0.0081 | 0.0015 | 0.0009 | 0.0036 | 0.0002 | 0.0069 | 0.0061 | 0.0014 | 0.0011 | 0.0005 | 0.0002 | 0.0018 | 0.0007 | 0.0012 | 0.0004 | 0.0003 | 0.0002 |
Cell Periphery | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.1396 | 0.247 | 0.1899 | 0.15 | 0.1229 | 0.2613 | 0.1924 | 0.1897 | 0.1879 | 0.0678 | 0.1233 | 0.18 | 0.2278 | 0.2521 | 0.2267 | 0.2022 | 0.2743 | 0.2338 |
Cytoplasmic Foci | 0.1339 | 0.1152 | 0.1442 | 0.1625 | 0.1479 | 0.124 | 0.1489 | 0.1025 | 0.1289 | 0.1059 | 0.1694 | 0.1387 | 0.1253 | 0.1201 | 0.107 | 0.1635 | 0.1095 | 0.1547 |
Eisosomes | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.007 | 0.0043 | 0.0071 | 0.0061 | 0.0056 | 0.0045 | 0.0069 | 0.0043 | 0.004 | 0.0027 | 0.0038 | 0.0039 | 0.0049 | 0.0057 | 0.0104 | 0.0028 | 0.0064 | 0.0035 |
Endosome | 0.2744 | 0.2954 | 0.352 | 0.4156 | 0.3644 | 0.3337 | 0.3214 | 0.2442 | 0.3032 | 0.5206 | 0.4077 | 0.3219 | 0.3476 | 0.357 | 0.3427 | 0.4634 | 0.3644 | 0.3628 |
Golgi | 0.0191 | 0.0123 | 0.0183 | 0.015 | 0.0208 | 0.0082 | 0.0119 | 0.0096 | 0.0129 | 0.0212 | 0.014 | 0.0139 | 0.0202 | 0.0189 | 0.0123 | 0.0203 | 0.0222 | 0.0137 |
Lipid Particles | 0.0236 | 0.0064 | 0.0091 | 0.0116 | 0.0139 | 0.007 | 0.0078 | 0.0095 | 0.0076 | 0.0054 | 0.0054 | 0.0113 | 0.0044 | 0.0057 | 0.0037 | 0.0041 | 0.0029 | 0.0065 |
Mitochondria | 0.013 | 0.0077 | 0.0131 | 0.0105 | 0.019 | 0.0045 | 0.0032 | 0.0152 | 0.008 | 0.0392 | 0.0082 | 0.016 | 0.0114 | 0.0063 | 0.0066 | 0.0066 | 0.0064 | 0.0045 |
None | 0.2583 | 0.2424 | 0.1922 | 0.0877 | 0.2296 | 0.1925 | 0.2392 | 0.3382 | 0.2845 | 0.1587 | 0.2012 | 0.2588 | 0.2189 | 0.1873 | 0.2375 | 0.0517 | 0.1658 | 0.184 |
Nuclear Periphery | 0.0091 | 0.0018 | 0.0054 | 0.0151 | 0.0041 | 0.0047 | 0.0053 | 0.0027 | 0.0032 | 0.0035 | 0.0019 | 0.0067 | 0.0013 | 0.0031 | 0.0038 | 0.0012 | 0.0022 | 0.0011 |
Nucleolus | 0.0008 | 0.0012 | 0.0007 | 0.0013 | 0.0005 | 0.0004 | 0.0012 | 0.0005 | 0.0009 | 0.0004 | 0.0005 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0002 |
Nucleus | 0.0122 | 0.0023 | 0.0024 | 0.0126 | 0.0022 | 0.0037 | 0.0036 | 0.0016 | 0.0019 | 0.001 | 0.0012 | 0.0025 | 0.0013 | 0.0017 | 0.0018 | 0.001 | 0.0013 | 0.0008 |
Peroxisomes | 0.013 | 0.0051 | 0.004 | 0.005 | 0.0053 | 0.0024 | 0.0025 | 0.0041 | 0.0093 | 0.003 | 0.0242 | 0.0026 | 0.002 | 0.0018 | 0.0026 | 0.0033 | 0.0022 | 0.0035 |
Punctate Nuclear | 0.0077 | 0.0016 | 0.0021 | 0.0083 | 0.003 | 0.0042 | 0.002 | 0.0011 | 0.0043 | 0.0005 | 0.0012 | 0.0014 | 0.0006 | 0.0007 | 0.0008 | 0.0017 | 0.0004 | 0.0011 |
Vacuole | 0.0326 | 0.0359 | 0.0273 | 0.0602 | 0.0151 | 0.0336 | 0.0325 | 0.0543 | 0.0265 | 0.0418 | 0.0281 | 0.0288 | 0.0252 | 0.0301 | 0.0305 | 0.0538 | 0.0345 | 0.0212 |
Vacuole Periphery | 0.006 | 0.0063 | 0.0067 | 0.0113 | 0.0042 | 0.0092 | 0.0052 | 0.0098 | 0.0103 | 0.0197 | 0.0073 | 0.0096 | 0.005 | 0.0065 | 0.0097 | 0.0154 | 0.0062 | 0.0068 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.4465 | 16.153 | 6.9597 | 14.4795 | 10.5803 | 12.2338 | 12.6444 | 14.0457 | 13.1786 | 16.5792 |
Translational Efficiency | 0.3904 | 0.375 | 0.7632 | 0.449 | 0.5157 | 0.4622 | 0.5614 | 0.4114 | 0.3864 | 0.3166 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
132 | 1111 | 214 | 1632 | 2422 | 1896 | 1744 | 70 | 2554 | 3007 | 1958 | 1702 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 863.12 | 779.45 | 871.23 | 845.86 | 732.78 | 786.31 | 789.59 | 912.22 | 739.52 | 783.78 | 798.51 | 848.59 |
Standard Deviation | 130.22 | 104.07 | 89.99 | 108.24 | 88.37 | 99.40 | 91.54 | 134.88 | 95.47 | 101.20 | 94.85 | 110.26 |
Intensity Change Log 2 | — | -0.147105 | 0.013492 | -0.029142 | — | 0.101718 | 0.107724 | 0.316002 | — | -0.027507 | 0.057525 | 0.139630 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.007517 | 0.000957 | 0.001543 | 0.002587 | 0.000229 | 0.000688 | 0.004599 | 0.003071 | 0.000606 | 0.000787 | 0.004265 | 0.002607 |
Bud Neck | 0.012959 | 0.009450 | 0.013024 | 0.016050 | 0.012853 | 0.012679 | 0.015234 | 0.007330 | 0.012859 | 0.011486 | 0.014992 | 0.015691 |
Bud Site | 0.043579 | 0.012819 | 0.014493 | 0.057745 | 0.010324 | 0.012658 | 0.035380 | 0.032996 | 0.012042 | 0.012718 | 0.033097 | 0.056727 |
Cell Periphery | 0.001749 | 0.000121 | 0.000164 | 0.000100 | 0.000070 | 0.000107 | 0.000323 | 0.000791 | 0.000157 | 0.000112 | 0.000305 | 0.000128 |
Cytoplasm | 0.139890* | 0.072461 | 0.142310* | 0.332965* | 0.104731* | 0.081314 | 0.120288* | 0.132298* | 0.106548* | 0.078043 | 0.122695* | 0.324712* |
Cytoplasmic Foci | 0.152289 | 0.397041* | 0.208793* | 0.148074 | 0.295554* | 0.340748* | 0.326429* | 0.328983* | 0.288149* | 0.361547* | 0.313572* | 0.155514 |
Eisosomes | 0.000137 | 0.000164 | 0.000142 | 0.000062 | 0.000095 | 0.000195 | 0.000089 | 0.000310 | 0.000097 | 0.000184 | 0.000094 | 0.000072 |
Endoplasmic Reticulum | 0.002210 | 0.001544 | 0.006122 | 0.002098 | 0.002579 | 0.001565 | 0.001923 | 0.001769 | 0.002560 | 0.001557 | 0.002382 | 0.002085 |
Endosome | 0.094030 | 0.231853* | 0.159781 | 0.042543 | 0.225448* | 0.236961* | 0.160132 | 0.129167 | 0.218655* | 0.235074* | 0.160094 | 0.046105 |
Golgi | 0.016314 | 0.092788 | 0.006393 | 0.006034 | 0.037984 | 0.105738 | 0.033784 | 0.012720 | 0.036864 | 0.100953 | 0.030790 | 0.006309 |
Lipid Particles | 0.009054 | 0.013331 | 0.029553 | 0.006305 | 0.014209 | 0.016933 | 0.017597 | 0.011674 | 0.013943 | 0.015602 | 0.018904 | 0.006525 |
Mitochondria | 0.007016 | 0.017079 | 0.002323 | 0.003532 | 0.019184 | 0.037794 | 0.007628 | 0.006766 | 0.018555 | 0.030140 | 0.007048 | 0.003665 |
Mitotic Spindle | 0.003704 | 0.003534 | 0.038705 | 0.068555 | 0.004904 | 0.004133 | 0.049423 | 0.029804 | 0.004842 | 0.003912 | 0.048252 | 0.066961 |
None | 0.000240 | 0.000593 | 0.001306 | 0.001593 | 0.000541 | 0.000276 | 0.000314 | 0.000617 | 0.000525 | 0.000393 | 0.000423 | 0.001553 |
Nuclear Periphery | 0.000932 | 0.000151 | 0.005228 | 0.001798 | 0.000341 | 0.000160 | 0.000830 | 0.000636 | 0.000372 | 0.000157 | 0.001310 | 0.001751 |
Nuclear Periphery Foci | 0.001485 | 0.000342 | 0.012193 | 0.002410 | 0.000234 | 0.000335 | 0.001792 | 0.002473 | 0.000298 | 0.000338 | 0.002929 | 0.002413 |
Nucleolus | 0.001630 | 0.000477 | 0.004296 | 0.001284 | 0.000773 | 0.000500 | 0.000827 | 0.000375 | 0.000817 | 0.000492 | 0.001206 | 0.001246 |
Nucleus | 0.065756 | 0.002680 | 0.098082 | 0.058560 | 0.007210 | 0.003493 | 0.014214 | 0.018626 | 0.010236 | 0.003193 | 0.023380 | 0.056917 |
Peroxisomes | 0.012696 | 0.016339 | 0.037386 | 0.023540 | 0.009029 | 0.015955 | 0.047622 | 0.068378 | 0.009218 | 0.016097 | 0.046503 | 0.025385 |
Vacuole | 0.410681* | 0.105677 | 0.213108* | 0.221616* | 0.233607* | 0.107124 | 0.158420 | 0.209986* | 0.242759* | 0.106590 | 0.164397 | 0.221138* |
Vacuole Periphery | 0.016130 | 0.020598 | 0.005055 | 0.002551 | 0.020101 | 0.020644 | 0.003154 | 0.001232 | 0.019896 | 0.020627 | 0.003362 | 0.002497 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.99 | 0.86 | 0.83 | -1.91 | -0.29 | -4.69 | -5.90 | -2.75 | -2.09 | 2.12 | -1.21 | -5.32 | -3.17 | -2.89 | 3.45 |
Bud Neck | 0.20 | -0.70 | -1.46 | -4.57 | -1.17 | 0.17 | -2.77 | 5.68 | 4.86 | 7.35 | 1.34 | -2.75 | -2.16 | -3.26 | 0.44 |
Bud Site | 1.92 | 1.78 | -0.21 | -7.87 | -5.46 | -1.80 | -8.43 | -1.48 | -1.24 | 0.78 | -1.00 | -7.72 | -8.27 | -8.08 | -1.43 |
Cell Periphery | 1.17 | 1.12 | 1.22 | 2.49 | 3.99 | -4.21 | -1.07 | -1.14 | -1.04 | -0.51 | 0.35 | -0.77 | 0.64 | 0.88 | 0.97 |
Cytoplasm | 4.80 | 0.62 | -7.71 | -32.59 | -11.52 | 5.29 | -4.62 | -2.15 | -3.03 | -1.18 | 7.51 | -4.64 | -28.48 | -33.90 | -23.08 |
Cytoplasmic Foci | -17.62 | -3.19 | 2.02 | 40.90 | 7.02 | -7.28 | -2.00 | 1.91 | 3.49 | 2.36 | -13.45 | -1.38 | 31.10 | 46.91 | 30.07* |
Eisosomes | -1.50 | -0.99 | 3.86 | 4.97 | 7.59 | -8.01 | 1.30 | -0.97 | -0.18 | -1.03 | -7.46 | 0.56 | 4.64 | 9.82 | 4.35 |
Endoplasmic Reticulum | 1.60 | -5.33 | 0.44 | -2.57 | 6.29 | 2.57 | 1.61 | 1.15 | -0.81 | 0.06 | 2.89 | 0.16 | 1.48 | -2.67 | 2.15 |
Endosome | -11.84 | -3.67 | 3.81 | 36.14* | 9.12 | -2.08 | 14.20 | 8.01 | 8.66 | 3.23 | -3.55 | 13.54 | 45.93* | 54.39* | 26.72* |
Golgi | -11.27 | 1.04 | 1.39 | 20.84 | 1.59 | -17.41 | 3.72 | 8.52 | 20.72 | 6.10 | -20.42 | 4.57 | 19.56 | 34.81 | 14.67 |
Lipid Particles | -2.61 | -4.94 | 1.55 | 6.15 | 6.14 | -2.07 | -2.90 | 1.58 | 2.52 | 2.80 | -1.64 | -4.29 | 11.11 | 10.62 | 13.53 |
Mitochondria | -3.17 | 0.79 | 0.73 | 10.53 | -0.52 | -8.78 | 10.17 | 4.07 | 8.41 | 0.88 | -7.22 | 10.54 | 15.59 | 20.89 | 7.39 |
Mitotic Spindle | 0.44 | -3.86 | -11.23 | -13.01 | -1.46 | 0.66 | -13.05 | -1.99 | -2.07 | 2.26 | 0.93 | -13.57 | -12.72 | -13.19 | -0.61 |
None | -0.42 | -2.38 | -5.85 | -2.80 | -1.06 | 1.17 | 0.89 | -0.75 | -1.30 | -1.18 | 0.55 | 0.37 | -4.42 | -5.60 | -6.41 |
Nuclear Periphery | 4.83 | -4.43 | -6.73 | -12.55 | 2.47 | 2.41 | -4.99 | -3.31 | -4.45 | -0.05 | 3.11 | -6.76 | -11.16 | -12.73 | -5.28 |
Nuclear Periphery Foci | 4.76 | -5.45 | -1.76 | -8.05 | 5.14 | -0.69 | -8.33 | -3.99 | -3.74 | -1.29 | -0.27 | -9.41 | -8.57 | -7.98 | 1.85 |
Nucleolus | 2.59 | -4.57 | -0.61 | -6.74 | 4.80 | 1.50 | -1.21 | 1.51 | 0.13 | 2.72 | 2.07 | -3.19 | -4.30 | -6.70 | -1.35 |
Nucleus | 5.10 | -2.68 | -1.84 | -26.03 | 1.83 | 4.95 | -7.20 | -3.72 | -4.30 | -1.99 | 7.76 | -8.36 | -23.20 | -25.99 | -15.36 |
Peroxisomes | -1.99 | -3.59 | -1.23 | 1.14 | 3.21 | -5.80 | -15.19 | -3.07 | -2.46 | 0.07 | -7.14 | -15.66 | -4.23 | 0.41 | 11.06 |
Vacuole | 12.25 | 6.90 | 5.01 | -24.39 | -4.54 | 20.67 | 6.92 | -2.72 | -6.05 | -4.00 | 24.11 | 7.92 | -7.70 | -27.77 | -14.39 |
Vacuole Periphery | -1.43 | 1.06 | 1.49 | 14.82 | 1.91 | -0.57 | 23.43 | 24.94 | 22.02 | 4.60 | -1.03 | 21.20 | 23.20 | 25.53 | 4.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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