Standard name
Human Ortholog
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.07 0 – 0 0.06 0 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.05 0 0.06 – 0 0 0 0 0.2 0.2 0.21 0.17 0.08 0.1 0 0 0 0.06 0.06 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.1 0 0.08 0.05 0.05 0 0.05 – 0.05 0 0.05 0 0.25 0.28 0.37 0.43 0.44 0.46 0 0 0 0.08 0.07 0.1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.1 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.05 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 0.44 0.28 0.27 0.26 0.37 0.31 – 0.39 0.33 0.44 0.43 0.27 0.24 0.2 0.14 0.22 0.26 0 0.07 0 0 0 0
Peroxisomes 0.09 0 0 0 0 0 0 – 0.07 0 0.05 0 0.15 0.2 0.29 0.11 0.06 0.16 0 0 0 0 0 0
SpindlePole 0.9 0.82 0.87 0.88 0.85 0.78 0.83 – 0.77 0.81 0.8 0.77 0.62 0.53 0.53 0.58 0.69 0.64 0.89 0.8 0.86 0.75 0.74 0.58
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 0 0 – 0 0 0 0 0 1 0 0 2 0 0 0 1 2 3 4
Bud 3 3 1 6 8 17 10 – 9 22 8 19 1 2 0 1 1 0 4 3 3 7 10 21
Bud Neck 2 13 4 7 12 19 10 – 17 16 14 16 4 4 2 0 1 1 1 6 2 4 3 8
Bud Site 11 6 0 8 5 21 6 – 18 39 16 35 2 3 0 0 1 1 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 10 20 20 24 12 26 – 14 20 12 13 89 71 88 15 14 19 0 2 4 21 31 65
Endoplasmic Reticulum 0 3 0 0 0 0 0 – 0 0 0 1 0 0 0 3 2 8 0 0 0 0 0 0
Endosome 41 19 36 18 23 6 22 – 28 20 19 9 108 99 157 38 76 90 13 23 15 30 35 53
Golgi 0 0 3 0 0 0 0 – 0 0 0 0 0 0 1 0 1 0 5 1 5 0 5 4
Mitochondria 25 22 3 2 3 30 5 – 22 34 16 20 2 3 3 0 3 6 4 6 7 6 8 14
Nucleus 9 27 10 13 27 6 10 – 16 17 14 21 15 13 10 2 3 2 2 10 3 3 2 4
Nuclear Periphery 0 4 0 0 1 0 0 – 0 0 0 1 0 0 0 1 0 0 0 2 0 0 0 3
Nucleolus 212 244 119 103 128 114 133 – 208 218 159 202 118 82 86 12 38 51 5 39 11 5 8 15
Peroxisomes 38 15 7 11 14 4 17 – 39 24 17 13 66 68 123 10 11 31 3 6 0 2 2 1
SpindlePole 377 453 368 330 425 238 354 – 414 532 287 363 275 186 227 51 118 124 379 462 386 282 358 308
Vac/Vac Membrane 1 3 2 2 1 0 0 – 1 0 0 2 6 3 8 8 9 3 1 3 1 3 5 10
Unique Cell Count 421 553 424 375 499 306 426 536 655 361 473 440 348 426 88 171 194 426 576 449 376 483 528
Labelled Cell Count 725 822 573 520 672 467 593 786 942 562 715 686 535 705 141 281 336 426 576 449 376 483 528


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.6 6.4 5.8 6.5 4.5 5.5 – 5.4 5.3 5.6 5.4 5.9 6.1 6.1 7.1 7.7 7.0 4.9 5.2 5.3
Std Deviation (1e-4) 1.1 1.1 1.5 1.2 1.6 1.3 1.4 – 1.3 1.3 1.3 1.4 1.5 1.7 1.7 1.5 1.8 1.6 1.6 1.6 1.3
Intensity Change (Log2) – – – -0.15 0.01 -0.52 -0.24 – -0.26 -0.29 -0.2 -0.24 -0.12 -0.08 -0.08 0.14 0.26 0.13 -0.38 -0.3 -0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.7 – 0 3.5 0 3.8 0 0 0 0 0 0
Bud Neck 1.1 1.7 0 1.6 – 2.3 1.8 2.7 2.5 0 0 0 0 0 0
Bud Site 0 0 0 0 – 3.8 5.1 4.4 5.7 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.1 -0.5 0.9 – -1.8 -1.4 -1.0 -1.6 6.9 6.7 7.0 4.2 1.7 2.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -2.1 -2.4 0 -1.9 – -2.0 -3.9 -1.8 -4.5 6.3 7.3 9.9 8.4 10.2 10.9
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.3 4.0 3.4 3.3 0 0 0 0 0 0
Nucleus 0.9 2.4 -0.4 0 – 0.6 0.2 1.2 1.7 0.9 1.1 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.2 -0.8 0 1.0 – 3.5 1.8 4.7 4.6 -0.4 -1.4 -2.7 -2.8 -1.5 -0.5
Peroxisomes 1.2 1.2 0 2.1 – 4.1 1.9 2.5 1.1 7.1 8.4 11.0 0 3.1 6.9
SpindlePole 0.5 -0.7 0 -1.5 – -3.8 -2.4 -2.7 -3.9 -8.2 -10.2 -10.7 -6.4 -5.1 -6.6
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.954 1.3141 1.4438 1.3682 1.1777 1.366 3.8442 2.5176 2.7451 2.9722 2.5136 2.4721 5.5892 5.9342 5.8532 6.936 5.698 5.3424
Actin 0.0163 0 0.0007 0.003 0.0012 0.0038 0.0314 0 0 0.0037 0 0.0028 0 0 0 0 0 0
Bud 0.0005 0.0001 0.0002 0.0004 0.0001 0 0.0003 0 0 0.0004 0 0 0 0.0003 0 0 0 0
Bud Neck 0.0024 0.0008 0.0024 0.0005 0.0007 0.0005 0.0038 0.0005 0.0005 0.0392 0.0002 0.0003 0.0003 0.0009 0.0001 0.0016 0.0003 0.0002
Bud Periphery 0.0009 0 0.0002 0.0007 0.0001 0.0001 0.0006 0 0.0001 0.0004 0 0.0001 0 0.0003 0 0 0 0
Bud Site 0.0017 0.0026 0.0004 0.0024 0.001 0.0002 0.0015 0.0008 0.0003 0.0032 0.0001 0.0001 0.0001 0.0024 0.0002 0.0001 0.0001 0
Cell Periphery 0.0002 0 0.0001 0.0002 0 0 0.0004 0 0.0001 0.0002 0 0 0 0.0001 0 0 0 0
Cytoplasm 0.0054 0.0003 0.002 0.0049 0.003 0.0001 0.0025 0.0001 0 0.0014 0 0.0001 0 0.0016 0 0 0 0
Cytoplasmic Foci 0.0239 0.0082 0.0053 0.0311 0.0821 0.002 0.0162 0.0024 0.0023 0.0124 0.0127 0.0019 0.0022 0.0079 0.0013 0.0299 0.0273 0.0007
Eisosomes 0.0001 0 0.0001 0.0001 0 0 0.0004 0 0 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0 0.0007 0.0013 0.0009 0.0002 0.0085 0 0 0.0009 0 0.0001 0 0.0005 0 0 0 0
Endosome 0.0237 0.0001 0.0063 0.0138 0.0253 0.002 0.0167 0.0004 0.0004 0.013 0.0002 0.0012 0 0.0029 0 0.0003 0.0002 0
Golgi 0.0068 0.0001 0.0011 0.0046 0.0031 0.0016 0.0068 0.0002 0.0001 0.0035 0.0003 0.0011 0.0001 0.0003 0 0.0005 0.0006 0
Lipid Particles 0.0184 0.0011 0.0055 0.0142 0.0478 0.0021 0.0154 0.0023 0.0013 0.011 0.0036 0.0023 0.0004 0.0048 0.0001 0.0044 0.0115 0.0001
Mitochondria 0.0058 0.0001 0.0045 0.0049 0.0009 0.0026 0.003 0 0.0001 0.0012 0 0.0001 0 0.0002 0 0 0 0
None 0.0052 0.0003 0.0029 0.0065 0.0024 0.0001 0.0041 0 0 0.0017 0 0.0001 0 0.0002 0 0 0 0
Nuclear Periphery 0.0105 0.0004 0.0044 0.0021 0.0088 0.0031 0.021 0.0005 0.0003 0.0035 0.0001 0.0004 0.0001 0.0011 0.0001 0.0003 0.0002 0.0001
Nucleolus 0.0869 0.1092 0.0586 0.0255 0.0465 0.0479 0.0165 0.0981 0.0612 0.0731 0.0109 0.0224 0.0079 0.0931 0.0717 0.0206 0.0397 0.0491
Nucleus 0.039 0.0726 0.0233 0.0341 0.0045 0.0123 0.0336 0.0679 0.0205 0.037 0.0008 0.009 0.0027 0.0501 0.0498 0.0044 0.0017 0.0123
Peroxisomes 0.0058 0.0002 0.0012 0.0055 0.0055 0.0005 0.0043 0.0002 0.0001 0.0025 0.0002 0.0016 0.0001 0.0003 0 0.0004 0.0006 0
Punctate Nuclear 0.7392 0.8038 0.8787 0.8347 0.7647 0.9205 0.8106 0.8265 0.9118 0.7894 0.9709 0.9562 0.9861 0.816 0.8764 0.9373 0.9177 0.9374
Vacuole 0.0038 0.0001 0.0008 0.0084 0.0006 0.0002 0.0014 0.0001 0.0008 0.0016 0 0.0001 0 0.0166 0 0 0 0
Vacuole Periphery 0.0019 0 0.0007 0.0013 0.0005 0.0003 0.0011 0 0.0001 0.0005 0 0 0 0.0006 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.6798 22.2157 24.178 11.089 32.221 9.9351 32.1959 17.6873 19.5204 24.3987
Translational Efficiency 0.7841 0.9145 0.2255 0.6206 0.5618 2.2923 0.6679 0.9689 0.6113 0.7711

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
239 956 1832 1612 1578 1306 2046 1807 1817 2262 3878 3419

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 670.70 794.80 890.91 705.88 690.48 805.66 787.56 876.40 687.88 801.07 836.38 796.00
Standard Deviation 117.79 131.51 121.92 106.26 99.67 109.93 138.31 127.46 102.46 119.65 140.63 145.45
Intensity Change Log 2 — 0.244924 0.409612 0.073755 — 0.222572 0.189790 0.343990 — 0.233629 0.302289 0.217147

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001061 0.000486 0.000083 0.000679 0.000325 0.001208 0.000284 0.000118 0.000422 0.000903 0.000189 0.000383
Bud Neck 0.009919 0.004181 0.000740 0.009289 0.002755 0.005357 0.009124 0.003127 0.003697 0.004860 0.005164 0.006033
Bud Site 0.010306 0.006775 0.000987 0.025232 0.003390 0.008702 0.007972 0.006285 0.004300 0.007888 0.004672 0.015218
Cell Periphery 0.000660 0.000216 0.000015 0.000342 0.000220 0.000317 0.000815 0.000047 0.000278 0.000274 0.000437 0.000186
Cytoplasm 0.041379 0.015209 0.001724 0.167557* 0.014537 0.033814 0.038345 0.018109 0.018068 0.025951 0.021045 0.088571
Cytoplasmic Foci 0.123780 0.061622 0.000769 0.046961 0.036832 0.070469 0.029833 0.012422 0.048269 0.066730 0.016103 0.028706
Eisosomes 0.000148 0.000110 0.000008 0.000041 0.000139 0.000090 0.000597 0.000008 0.000140 0.000098 0.000319 0.000024
Endoplasmic Reticulum 0.003862 0.001993 0.000209 0.001161 0.001639 0.001636 0.001116 0.000216 0.001931 0.001787 0.000688 0.000662
Endosome 0.015463 0.009545 0.000248 0.009712 0.005072 0.013019 0.002829 0.002025 0.006439 0.011551 0.001610 0.005650
Golgi 0.015039 0.013966 0.000340 0.001844 0.009494 0.010442 0.002129 0.001013 0.010224 0.011931 0.001284 0.001405
Lipid Particles 0.053361 0.009938 0.000143 0.003980 0.009168 0.024424 0.003180 0.000576 0.014981 0.018302 0.001745 0.002181
Mitochondria 0.005224 0.003107 0.000108 0.002476 0.001441 0.003917 0.000908 0.000504 0.001938 0.003575 0.000530 0.001434
Mitotic Spindle 0.453951* 0.661410* 0.971548* 0.552625* 0.630305* 0.598821* 0.712135* 0.926660* 0.607108* 0.625274* 0.834684* 0.750309*
None 0.011504 0.004940 0.000319 0.027741 0.013406 0.010639 0.026443 0.001648 0.013156 0.008230 0.014102 0.013951
Nuclear Periphery 0.000865 0.000457 0.000116 0.001054 0.000443 0.000733 0.000519 0.000232 0.000498 0.000616 0.000329 0.000620
Nuclear Periphery Foci 0.106011 0.106890 0.004600 0.020865 0.094805 0.057040 0.022810 0.003793 0.096279 0.078109 0.014207 0.011842
Nucleolus 0.045443 0.046019 0.010153 0.018709 0.120541 0.075172 0.059233 0.005911 0.110663 0.062851 0.036047 0.011945
Nucleus 0.053540 0.022006 0.005966 0.087014 0.031738 0.056494 0.072575 0.011605 0.034606 0.041918 0.041109 0.047159
Peroxisomes 0.043688 0.028593 0.001327 0.008599 0.022745 0.025302 0.006937 0.002720 0.025499 0.026693 0.004287 0.005492
Vacuole 0.004015 0.001900 0.000552 0.013145 0.000849 0.001908 0.001935 0.002150 0.001265 0.001905 0.001282 0.007334
Vacuole Periphery 0.000782 0.000636 0.000044 0.000972 0.000158 0.000496 0.000282 0.000829 0.000240 0.000555 0.000169 0.000897

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.82 3.12 0.30 -3.50 -6.78 -3.32 -1.51 2.41 4.24 4.59 -2.95 1.50 -2.06 1.58 -4.10
Bud Neck 1.73 2.53 -1.02 -6.97 -9.59 -4.92 -7.69 -5.08 -1.69 3.69 -2.74 -5.19 -7.98 -6.30 -2.20
Bud Site 0.02 2.17 -5.64 -7.55 -9.98 -4.00 -7.47 -8.29 -4.42 -1.39 -4.67 -4.89 -12.00 -8.62 -8.46
Cell Periphery 1.51 2.50 1.47 -0.21 -7.37 -0.94 -4.10 8.70 7.32 5.57 0.59 -1.56 3.28 4.32 3.66
Cytoplasm 3.34 4.84 -8.92 -16.92 -19.38 -3.86 -7.61 -4.23 -0.32 2.76 -1.70 -2.20 -15.07 -13.89 -14.23
Cytoplasmic Foci 6.87 11.34 7.27 0.70 -16.14 -5.23 4.05 7.23 11.32 3.55 -1.51 12.33 6.24 8.47 -8.87
Eisosomes 1.20 10.97 8.30 11.68 -10.03 5.17 -1.09 14.60 21.10 2.23 5.04 -0.17 14.49 22.52 2.05
Endoplasmic Reticulum 1.23 3.99 2.29 3.65 -7.09 3.95 5.39 15.80 11.12 4.89 2.52 9.48 9.52 8.90 -0.80
Endosome 3.35 7.68 2.91 -1.16 -14.82 -3.60 3.59 4.92 6.26 1.99 -2.75 9.24 1.02 3.71 -10.31
Golgi -0.68 5.84 5.37 10.08 -6.43 3.29 14.46 17.08 13.26 6.48 1.06 16.96 17.62 15.76 2.58
Lipid Particles 7.13 8.17 7.71 4.02 -10.57 -3.71 4.83 7.30 7.44 4.73 2.00 9.51 8.82 8.28 -3.80
Mitochondria 2.34 4.28 2.75 -0.07 -7.41 -2.37 -0.26 1.21 3.39 2.79 -1.11 3.01 0.79 1.80 -4.39
Mitotic Spindle -8.86 -20.54* -7.46 2.88 29.14* -2.85 -10.92 -26.45* -21.61 -15.92 -6.22 -24.58 -17.62 -11.88 7.97
None 1.99 3.26 -2.10 -7.33 -9.85 1.83 -3.67 6.60 5.79 8.12 3.25 -0.02 0.45 -3.33 0.47
Nuclear Periphery 5.30 7.90 -3.09 -8.71 -11.13 -3.09 -3.47 -0.62 0.90 0.71 -1.10 2.52 -5.13 -4.31 -6.13
Nuclear Periphery Foci -0.74 7.83 5.74 11.90 -8.54 6.02 16.56 22.81* 16.24 9.15 3.75 20.68 20.96 19.11 0.64
Nucleolus 1.26 3.06 2.02 1.35 -2.28 7.33 10.02 21.20 13.78 14.58 9.92 13.50 19.05 11.71 9.60
Nucleus 3.19 3.51 -3.20 -11.58 -12.51 -2.54 -9.20 0.80 2.92 9.10 0.09 -4.48 -6.48 -6.38 -2.31
Peroxisomes 1.97 6.82 5.69 9.49 -7.50 0.46 9.85 12.98 11.90 5.09 0.43 13.59 13.09 14.92 -1.03
Vacuole 2.32 2.18 -7.76 -8.96 -8.85 -3.20 -5.36 -4.93 -4.34 -3.23 -2.81 -3.71 -10.34 -9.86 -9.15
Vacuole Periphery 0.79 2.77 0.08 -0.87 -4.40 -2.59 -3.54 -1.67 -1.12 -1.09 -1.66 -0.41 -2.52 -1.47 -2.43
Standard name
Human Ortholog
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
Localization
Cell Percentages spindle pole (70%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Spc25

Spc25


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc25-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available