Standard name
Human Ortholog
Description Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.31 0.22 0.2 0.22 0.25 0.2 0.22 0.2 0.24 0.22 0.13 0.11 0.09 0.05 0.12 0.1 0 0 0 0 0 0
Bud 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.84 0.8 0.89 0.84 0.75 0.67 0.63 0.5 0.54 0.55 0.58 0.89 0.92 0.91 0.69 0.56 0.78 0.91 0.82 0.95 0.95 0.94 0.91
Cytoplasm 0.1 0.22 0 0.1 0.06 0.12 0.09 0.12 0.12 0.13 0.09 0.19 0.18 0.23 0.37 0.42 0.23 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.06 0.06 0.05 0.06 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.1 0.35 0.37 0.36 0.49 0.54 0.58 0.63 0.68 0.67 0 0 0 0.09 0.1 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.08 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 57 97 50 84 111 110 136 134 145 126 33 25 23 8 30 24 1 6 5 2 7 7
Bud 6 14 9 2 7 7 7 16 19 22 5 7 0 6 4 7 8 1 0 1 0 0 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 9 1 0 3 7
Bud Site 1 0 2 0 1 1 0 2 1 0 0 0 0 0 0 5 0
Cell Periphery 113 148 391 207 280 301 347 308 351 334 335 222 208 222 106 145 179 156 171 466 217 343 351
Cytoplasm 14 40 16 24 23 55 49 74 81 79 51 48 41 57 57 108 53 1 10 1 0 4 2
Endoplasmic Reticulum 1 4 9 9 11 28 32 34 37 17 25 2 0 2 6 7 12 1 3 4 1 3 4
Endosome 2 0 1 0 0 1 1 1 1 0 0 0 0 0 0 0 0 1 0 1 0 0 2
Golgi 0 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 1 1 0 1
Mitochondria 32 18 152 90 133 222 294 360 415 412 386 2 0 0 14 25 15 1 0 3 2 1 3
Nucleus 0 0 0 0 1 2 2 1 3 0 4 0 0 4 21 20 5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 1 0 12 6 8 9 0 0 0 0 1 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 2 1 10 2 9 5 3 1 3 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 1 2 1 1 2 1 1 2 1 1 0 3 13 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 6 3 14 6 17 14 20 0 0 0 0 1 3 3 4 0 0 0 0
Unique Cell Count 134 186 437 246 374 452 549 622 656 602 578 249 227 245 154 259 229 173 209 489 230 365 388
Labelled Cell Count 189 281 681 386 558 735 868 958 1069 1035 967 316 275 314 219 363 300 173 209 489 230 365 388


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 9.1 8.6 8.5 8.8 8.1 8.2 8.5 7.8 8.0 8.2 10.2 11.0 11.2 9.0 10.1 13.4 10.4 11.4 12.0
Std Deviation (1e-4) 1.3 1.6 1.3 1.5 1.7 1.8 1.6 1.9 1.9 2.0 2.1 1.4 1.5 1.6 2.1 2.5 3.2 1.6 2.0 2.0
Intensity Change (Log2) -0.02 0.02 -0.09 -0.07 -0.02 -0.15 -0.11 -0.07 0.25 0.35 0.38 0.07 0.23 0.64 0.27 0.41 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.6 0.1 0.8 -0.8 -0.1 -0.7 0.7 -0.2 -2.9 -3.5 -4.2 -4.7 -3.5 -3.7
Bud 0 -0.2 -0.6 -1.0 0.5 0.9 1.5 -1.6 0.6 0 0.3 0 0.5 1.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.0 -5.5 -8.2 -9.4 -13.5 -12.5 -11.8 -11.0 -0.1 0.9 0.5 -6.0 -10.1 -3.9
Cytoplasm 3.3 1.7 4.7 3.3 4.7 4.9 5.2 3.3 6.8 6.3 7.9 10.8 12.7 7.8
Endoplasmic Reticulum 1.3 0.8 3.1 2.9 2.8 2.9 0.8 2.0 0 0 0 0 0.5 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 0.2 4.3 5.9 7.4 9.2 10.8 10.1 -10.3 -10.1 -10.5 -6.1 -7.4 -8.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 7.9 5.9 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2.6 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4.4 0
Vacuole 0 0 0 3.4 0 3.4 3.2 3.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.256 6.5856 6.7617 5.4036 6.1066 6.707 6.6382 6.9143 6.6575 5.8136 6.2678 6.7138 6.9886 6.9123 6.5682 6.4741 5.7987 6.4009
Actin 0.0003 0.0002 0.0004 0.0013 0.0005 0.0001 0.0153 0.0007 0.0062 0.0392 0.0257 0.0053 0.0012 0.0001 0.0029 0 0.0002 0.0002
Bud 0.0003 0.0003 0.0015 0.0026 0.0007 0.0002 0.0005 0.0005 0.0004 0.0007 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001
Bud Neck 0.0007 0.0026 0.0054 0.0067 0.0092 0.0106 0.0009 0.0012 0.0027 0.0174 0.0007 0.012 0.0012 0.003 0.0063 0.0002 0.0069 0.0087
Bud Periphery 0.0048 0.0019 0.0116 0.0571 0.0169 0.0046 0.0056 0.0047 0.0065 0.006 0.0018 0.0055 0.0017 0.0015 0.0037 0.0048 0.0043 0.0028
Bud Site 0.0032 0.0734 0.0154 0.0014 0.0007 0.0007 0.0027 0.0219 0.0164 0.0046 0.0006 0.001 0.0043 0.0533 0.0141 0.0001 0.001 0.0007
Cell Periphery 0.8718 0.7957 0.8353 0.7161 0.848 0.9103 0.8615 0.8751 0.8727 0.8697 0.9023 0.9212 0.8611 0.881 0.8671 0.9264 0.9002 0.9252
Cytoplasm 0.0785 0.0995 0.1063 0.1385 0.0671 0.0494 0.0419 0.0631 0.0619 0.0324 0.0436 0.0293 0.0902 0.0496 0.0894 0.029 0.0458 0.0414
Cytoplasmic Foci 0.0001 0.0002 0.0001 0.0002 0.0172 0.0001 0.0015 0.0003 0.0013 0.0011 0.0005 0.0005 0.0008 0.0001 0.0002 0 0.0001 0.0001
Eisosomes 0.0022 0.0032 0.0009 0.0205 0.0049 0.0003 0.0085 0.0018 0.0022 0.0004 0.0051 0.0029 0.0016 0.0008 0.0007 0.0002 0.0017 0.0003
Endoplasmic Reticulum 0.0163 0.0143 0.0116 0.0359 0.0163 0.0124 0.0114 0.0127 0.0085 0.0099 0.0038 0.0056 0.0151 0.004 0.0073 0.0268 0.0269 0.0121
Endosome 0.0007 0 0.0001 0.0001 0.0001 0 0.002 0.0028 0.0007 0.0037 0.0009 0.0017 0.0002 0 0 0 0 0
Golgi 0.0001 0.0001 0.0001 0.0004 0.0004 0.0001 0.0031 0.001 0.0012 0.0038 0.0045 0.003 0.0004 0 0.0002 0 0.0001 0
Lipid Particles 0.0018 0.001 0.0007 0.0007 0.0026 0.0006 0.0063 0.0024 0.0012 0.002 0.0029 0.0007 0.0016 0.0002 0.0008 0.0005 0.0017 0.0005
Mitochondria 0.0004 0 0.0001 0.0008 0.0002 0 0.0144 0.0003 0.0046 0.0005 0.001 0.0015 0.0004 0.0003 0 0 0 0
None 0.0007 0.0007 0.0004 0.0027 0.0066 0.0002 0.0112 0.0002 0.0044 0.0004 0.0005 0.0003 0.0021 0.0002 0.0003 0.0001 0.0003 0.0001
Nuclear Periphery 0.0036 0.0007 0.0005 0.0017 0.0008 0.0004 0.001 0.001 0.0005 0.0008 0.0003 0.0003 0.0012 0.0004 0.0004 0.0009 0.001 0.0004
Nucleolus 0.0002 0.0001 0.0001 0.0001 0.0005 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0002 0 0.0001 0 0 0.0001 0
Nucleus 0.0015 0.0003 0.0004 0.0003 0.0004 0.0003 0.0002 0.0002 0.0002 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0001 0.0001
Peroxisomes 0 0 0 0 0 0 0.0024 0 0.0003 0.0007 0.0003 0.0001 0.0039 0 0 0 0 0
Punctate Nuclear 0 0 0 0 0.0003 0 0 0 0.0001 0.0003 0.0001 0 0 0 0 0 0 0
Vacuole 0.0125 0.0059 0.0089 0.0127 0.0065 0.0096 0.0092 0.0094 0.0077 0.0056 0.005 0.0077 0.0127 0.0051 0.0061 0.0108 0.0094 0.0071
Vacuole Periphery 0.0004 0 0.0001 0.0003 0.0002 0.0001 0.0003 0.0005 0.0001 0.0003 0.0001 0.0008 0.0001 0 0 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.4861 19.7275 15.1808 16.9992 16.4255 21.239 16.6221 23.4348 23.8148 22.5959
Translational Efficiency 0.9655 0.5259 0.7426 0.7973 0.8416 0.9662 1.0 0.8808 0.9968 0.7908

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
101 1080 1008 150 154 1318 2216 105 255 2398 3224 255

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1108.96 982.09 1321.35 1331.80 1024.51 992.75 1096.57 1205.82 1057.96 987.95 1166.85 1279.93
Standard Deviation 193.52 192.87 191.57 268.57 140.87 146.54 135.21 180.18 168.89 169.07 186.81 244.22
Intensity Change Log 2 -0.175280 0.252805 0.264170 -0.045432 0.098064 0.235080 -0.111466 0.180565 0.250274

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002939 0.004971 0.019581 0.008470 0.000699 0.002338 0.005406 0.023502 0.001587 0.003524 0.009838 0.014660
Bud Neck 0.011147 0.021347 0.015088 0.018137 0.003378 0.007956 0.017069 0.022098 0.006455 0.013987 0.016449 0.019768
Bud Site 0.056819 0.197079* 0.159272* 0.094901 0.027133 0.040521 0.059809 0.143636* 0.038891 0.111031* 0.090906 0.114968*
Cell Periphery 0.750960* 0.369852* 0.573286* 0.750180* 0.904697* 0.721731* 0.419996* 0.490872* 0.843805* 0.563253* 0.467923* 0.643406*
Cytoplasm 0.161109* 0.350328* 0.196037* 0.088116 0.028379 0.195726* 0.476017* 0.269776* 0.080950 0.265355* 0.388480* 0.162917*
Cytoplasmic Foci 0.002162 0.016942 0.002667 0.001161 0.003203 0.005698 0.001573 0.003015 0.002791 0.010762 0.001915 0.001924
Eisosomes 0.001444 0.000351 0.000935 0.003194 0.000071 0.000469 0.000846 0.003804 0.000615 0.000416 0.000874 0.003445
Endoplasmic Reticulum 0.002166 0.006094 0.013013 0.010840 0.014480 0.006834 0.002355 0.007956 0.009603 0.006501 0.005688 0.009652
Endosome 0.000922 0.007190 0.000790 0.001950 0.001491 0.002689 0.000624 0.001027 0.001266 0.004716 0.000676 0.001570
Golgi 0.001777 0.003401 0.001253 0.003680 0.000599 0.001953 0.000324 0.001127 0.001065 0.002605 0.000614 0.002629
Lipid Particles 0.000147 0.002654 0.001571 0.002884 0.000066 0.000434 0.001284 0.009759 0.000098 0.001434 0.001374 0.005715
Mitochondria 0.000116 0.000690 0.000440 0.000287 0.001413 0.004581 0.000962 0.000240 0.000900 0.002829 0.000798 0.000267
Mitotic Spindle 0.000435 0.000149 0.000298 0.000498 0.000212 0.000138 0.000142 0.000237 0.000300 0.000143 0.000191 0.000390
None 0.000008 0.000117 0.000033 0.000026 0.000019 0.000029 0.000090 0.000020 0.000015 0.000068 0.000072 0.000023
Nuclear Periphery 0.000095 0.000111 0.000379 0.000204 0.000085 0.000175 0.000174 0.000087 0.000089 0.000146 0.000238 0.000156
Nuclear Periphery Foci 0.000422 0.000483 0.000617 0.000508 0.000299 0.000171 0.000182 0.000828 0.000348 0.000312 0.000318 0.000640
Nucleolus 0.000007 0.000087 0.000033 0.000017 0.000012 0.000070 0.000066 0.000018 0.000010 0.000077 0.000056 0.000017
Nucleus 0.000361 0.000929 0.001593 0.000837 0.000143 0.000779 0.001539 0.001522 0.000229 0.000847 0.001556 0.001119
Peroxisomes 0.000248 0.001278 0.000844 0.000198 0.000119 0.001865 0.001316 0.000838 0.000170 0.001601 0.001168 0.000462
Vacuole 0.006391 0.014265 0.011239 0.012864 0.003454 0.004000 0.009513 0.019339 0.004617 0.008623 0.010053 0.015530
Vacuole Periphery 0.000324 0.001682 0.001031 0.001049 0.010049 0.001844 0.000713 0.000299 0.006197 0.001771 0.000812 0.000740

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.44 -10.06 -2.14 -1.41 3.82 -4.77 -11.12 -3.75 -3.48 -2.97 -4.90 -13.06 -4.44 -3.80 -1.62
Bud Neck -3.73 -1.63 -1.71 0.77 -0.88 -4.00 -9.24 -3.44 -2.60 -0.91 -5.62 -7.29 -4.18 -1.86 -1.08
Bud Site -11.30 -8.40 -2.29 6.58 4.18 -1.78 -4.41 -5.96 -5.56 -4.55 -10.20 -7.97 -6.13 -0.41 -2.16
Cell Periphery 13.22 6.27 0.05 -14.16 -6.69 9.45 26.09* 10.76 6.54 -1.89 16.13 22.72 7.61 -3.38 -7.72
Cytoplasm -8.44 -1.60 2.97 16.28 7.06 -20.13 -54.39* -9.10 -2.77 7.58 -16.59 -27.75 -4.75 6.56 14.48
Cytoplasmic Foci -6.66 -0.31 0.58 9.99 2.63 -1.32 0.95 -0.07 1.92 -1.92 -5.43 0.71 0.56 9.16 -0.29
Eisosomes 2.43 1.12 -2.29 -4.57 -3.62 -8.66 -7.72 -2.23 -1.99 -1.76 1.08 -1.33 -3.54 -3.89 -3.29
Endoplasmic Reticulum -4.05 -7.22 -2.07 -1.12 0.50 1.24 1.99 0.95 -0.30 -1.63 0.83 1.05 -0.00 -1.08 -1.38
Endosome -5.11 0.20 -0.76 3.40 -1.00 -1.40 1.40 0.54 2.26 -1.28 -4.75 1.27 -0.40 3.47 -1.32
Golgi -1.20 0.43 -0.64 -0.10 -0.90 -2.71 1.42 -1.51 1.51 -2.70 -2.50 0.91 -0.94 -0.01 -1.27
Lipid Particles -3.01 -7.66 -1.40 -0.12 -0.68 -4.20 -4.45 -1.88 -1.81 -1.65 -3.53 -6.49 -2.33 -1.76 -1.80
Mitochondria -4.96 -6.06 -1.33 2.43 1.17 -2.29 0.54 1.41 3.88 3.71 -2.44 0.20 1.25 4.12 3.84
Mitotic Spindle 1.38 0.66 -0.27 -3.36 -1.92 1.83 1.75 -0.74 -2.31 -2.25 1.83 1.28 -0.93 -3.95 -3.21
None -1.60 -9.03 -4.09 1.33 1.63 -0.89 -3.53 -0.35 0.45 3.30 -1.71 -4.29 -1.42 1.39 3.64
Nuclear Periphery -0.50 -7.23 -2.76 -2.76 4.27 -4.57 -5.00 -0.16 4.23 4.60 -3.29 -8.39 -2.85 -0.49 3.80
Nuclear Periphery Foci -0.82 -3.42 -1.18 -0.31 1.74 1.12 1.03 -2.82 -4.35 -4.28 0.47 0.41 -2.94 -4.36 -4.51
Nucleolus -2.02 -13.91 -4.56 1.78 6.27 -1.57 -13.88 -0.97 1.23 3.84 -2.51 -17.72 -1.88 2.13 7.28
Nucleus -4.10 -8.61 -2.49 0.39 3.30 -1.36 -12.50 -2.36 -1.21 -0.41 -2.32 -15.03 -3.22 -0.95 0.95
Peroxisomes -9.29 -6.63 0.62 12.53 11.40 -9.92 -14.72 -3.39 3.74 2.11 -13.24 -15.37 -3.03 8.30 6.62
Vacuole -4.67 -4.50 -2.37 0.39 -0.68 -0.27 -2.97 -3.20 -3.35 -2.20 -2.79 -4.17 -4.01 -2.79 -2.32
Vacuole Periphery -8.16 -3.87 -1.15 1.00 -0.03 1.52 1.74 1.81 5.67 4.92 1.36 1.65 1.66 2.58 0.19
Standard name
Human Ortholog
Description Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus
Localization
Cell Percentages cell periphery (81%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Gpa2

Gpa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available