Standard name | |
---|---|
Human Ortholog | |
Description | Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 19 | 57 | 97 | 50 | 84 | 111 | 110 | 136 | – | 134 | 145 | 126 | 33 | 25 | 23 | 8 | 30 | 24 | 1 | 6 | 5 | 2 | 7 | 7 |
Bud | 6 | 14 | 9 | 2 | 7 | 7 | 7 | 16 | – | 19 | 22 | 5 | 7 | 0 | 6 | 4 | 7 | 8 | 1 | 0 | 1 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 9 | 1 | 0 | 3 | 7 |
Bud Site | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | – | – | – | – | – | – |
Cell Periphery | 113 | 148 | 391 | 207 | 280 | 301 | 347 | 308 | – | 351 | 334 | 335 | 222 | 208 | 222 | 106 | 145 | 179 | 156 | 171 | 466 | 217 | 343 | 351 |
Cytoplasm | 14 | 40 | 16 | 24 | 23 | 55 | 49 | 74 | – | 81 | 79 | 51 | 48 | 41 | 57 | 57 | 108 | 53 | 1 | 10 | 1 | 0 | 4 | 2 |
Endoplasmic Reticulum | 1 | 4 | 9 | 9 | 11 | 28 | 32 | 34 | – | 37 | 17 | 25 | 2 | 0 | 2 | 6 | 7 | 12 | 1 | 3 | 4 | 1 | 3 | 4 |
Endosome | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 |
Mitochondria | 32 | 18 | 152 | 90 | 133 | 222 | 294 | 360 | – | 415 | 412 | 386 | 2 | 0 | 0 | 14 | 25 | 15 | 1 | 0 | 3 | 2 | 1 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | – | 3 | 0 | 4 | 0 | 0 | 4 | 21 | 20 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 12 | – | 6 | 8 | 9 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 2 | 1 | 10 | 2 | 9 | 5 | – | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 1 | 2 | 1 | 1 | 2 | – | 1 | 1 | 2 | 1 | 1 | 0 | 3 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 6 | 3 | 14 | 6 | – | 17 | 14 | 20 | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 4 | 0 | 0 | 0 | 0 |
Unique Cell Count | 134 | 186 | 437 | 246 | 374 | 452 | 549 | 622 | 656 | 602 | 578 | 249 | 227 | 245 | 154 | 259 | 229 | 173 | 209 | 489 | 230 | 365 | 388 | |
Labelled Cell Count | 189 | 281 | 681 | 386 | 558 | 735 | 868 | 958 | 1069 | 1035 | 967 | 316 | 275 | 314 | 219 | 363 | 300 | 173 | 209 | 489 | 230 | 365 | 388 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 9.1 | 8.6 | 8.5 | 8.8 | 8.1 | 8.2 | 8.5 | – | 7.8 | 8.0 | 8.2 | 10.2 | 11.0 | 11.2 | 9.0 | 10.1 | 13.4 | 10.4 | 11.4 | 12.0 |
Std Deviation (1e-4) | 1.3 | 1.6 | 1.3 | 1.5 | 1.7 | 1.8 | 1.6 | 1.9 | – | 1.9 | 2.0 | 2.1 | 1.4 | 1.5 | 1.6 | 2.1 | 2.5 | 3.2 | 1.6 | 2.0 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.02 | -0.09 | -0.07 | -0.02 | – | -0.15 | -0.11 | -0.07 | 0.25 | 0.35 | 0.38 | 0.07 | 0.23 | 0.64 | 0.27 | 0.41 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.6 | 0.1 | 0.8 | -0.8 | -0.1 | – | -0.7 | 0.7 | -0.2 | -2.9 | -3.5 | -4.2 | -4.7 | -3.5 | -3.7 |
Bud | 0 | -0.2 | -0.6 | -1.0 | 0.5 | – | 0.9 | 1.5 | -1.6 | 0.6 | 0 | 0.3 | 0 | 0.5 | 1.1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.0 | -5.5 | -8.2 | -9.4 | -13.5 | – | -12.5 | -11.8 | -11.0 | -0.1 | 0.9 | 0.5 | -6.0 | -10.1 | -3.9 |
Cytoplasm | 3.3 | 1.7 | 4.7 | 3.3 | 4.7 | – | 4.9 | 5.2 | 3.3 | 6.8 | 6.3 | 7.9 | 10.8 | 12.7 | 7.8 |
Endoplasmic Reticulum | 1.3 | 0.8 | 3.1 | 2.9 | 2.8 | – | 2.9 | 0.8 | 2.0 | 0 | 0 | 0 | 0 | 0.5 | 2.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.5 | 0.2 | 4.3 | 5.9 | 7.4 | – | 9.2 | 10.8 | 10.1 | -10.3 | -10.1 | -10.5 | -6.1 | -7.4 | -8.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 7.9 | 5.9 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2.6 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 0 |
Vacuole | 0 | 0 | 0 | 3.4 | 0 | – | 3.4 | 3.2 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.256 | 6.5856 | 6.7617 | 5.4036 | 6.1066 | 6.707 | 6.6382 | 6.9143 | 6.6575 | 5.8136 | 6.2678 | 6.7138 | 6.9886 | 6.9123 | 6.5682 | 6.4741 | 5.7987 | 6.4009 |
Actin | 0.0003 | 0.0002 | 0.0004 | 0.0013 | 0.0005 | 0.0001 | 0.0153 | 0.0007 | 0.0062 | 0.0392 | 0.0257 | 0.0053 | 0.0012 | 0.0001 | 0.0029 | 0 | 0.0002 | 0.0002 |
Bud | 0.0003 | 0.0003 | 0.0015 | 0.0026 | 0.0007 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0007 | 0.0026 | 0.0054 | 0.0067 | 0.0092 | 0.0106 | 0.0009 | 0.0012 | 0.0027 | 0.0174 | 0.0007 | 0.012 | 0.0012 | 0.003 | 0.0063 | 0.0002 | 0.0069 | 0.0087 |
Bud Periphery | 0.0048 | 0.0019 | 0.0116 | 0.0571 | 0.0169 | 0.0046 | 0.0056 | 0.0047 | 0.0065 | 0.006 | 0.0018 | 0.0055 | 0.0017 | 0.0015 | 0.0037 | 0.0048 | 0.0043 | 0.0028 |
Bud Site | 0.0032 | 0.0734 | 0.0154 | 0.0014 | 0.0007 | 0.0007 | 0.0027 | 0.0219 | 0.0164 | 0.0046 | 0.0006 | 0.001 | 0.0043 | 0.0533 | 0.0141 | 0.0001 | 0.001 | 0.0007 |
Cell Periphery | 0.8718 | 0.7957 | 0.8353 | 0.7161 | 0.848 | 0.9103 | 0.8615 | 0.8751 | 0.8727 | 0.8697 | 0.9023 | 0.9212 | 0.8611 | 0.881 | 0.8671 | 0.9264 | 0.9002 | 0.9252 |
Cytoplasm | 0.0785 | 0.0995 | 0.1063 | 0.1385 | 0.0671 | 0.0494 | 0.0419 | 0.0631 | 0.0619 | 0.0324 | 0.0436 | 0.0293 | 0.0902 | 0.0496 | 0.0894 | 0.029 | 0.0458 | 0.0414 |
Cytoplasmic Foci | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0172 | 0.0001 | 0.0015 | 0.0003 | 0.0013 | 0.0011 | 0.0005 | 0.0005 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 |
Eisosomes | 0.0022 | 0.0032 | 0.0009 | 0.0205 | 0.0049 | 0.0003 | 0.0085 | 0.0018 | 0.0022 | 0.0004 | 0.0051 | 0.0029 | 0.0016 | 0.0008 | 0.0007 | 0.0002 | 0.0017 | 0.0003 |
Endoplasmic Reticulum | 0.0163 | 0.0143 | 0.0116 | 0.0359 | 0.0163 | 0.0124 | 0.0114 | 0.0127 | 0.0085 | 0.0099 | 0.0038 | 0.0056 | 0.0151 | 0.004 | 0.0073 | 0.0268 | 0.0269 | 0.0121 |
Endosome | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.002 | 0.0028 | 0.0007 | 0.0037 | 0.0009 | 0.0017 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0031 | 0.001 | 0.0012 | 0.0038 | 0.0045 | 0.003 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0018 | 0.001 | 0.0007 | 0.0007 | 0.0026 | 0.0006 | 0.0063 | 0.0024 | 0.0012 | 0.002 | 0.0029 | 0.0007 | 0.0016 | 0.0002 | 0.0008 | 0.0005 | 0.0017 | 0.0005 |
Mitochondria | 0.0004 | 0 | 0.0001 | 0.0008 | 0.0002 | 0 | 0.0144 | 0.0003 | 0.0046 | 0.0005 | 0.001 | 0.0015 | 0.0004 | 0.0003 | 0 | 0 | 0 | 0 |
None | 0.0007 | 0.0007 | 0.0004 | 0.0027 | 0.0066 | 0.0002 | 0.0112 | 0.0002 | 0.0044 | 0.0004 | 0.0005 | 0.0003 | 0.0021 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Nuclear Periphery | 0.0036 | 0.0007 | 0.0005 | 0.0017 | 0.0008 | 0.0004 | 0.001 | 0.001 | 0.0005 | 0.0008 | 0.0003 | 0.0003 | 0.0012 | 0.0004 | 0.0004 | 0.0009 | 0.001 | 0.0004 |
Nucleolus | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0015 | 0.0003 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0024 | 0 | 0.0003 | 0.0007 | 0.0003 | 0.0001 | 0.0039 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0125 | 0.0059 | 0.0089 | 0.0127 | 0.0065 | 0.0096 | 0.0092 | 0.0094 | 0.0077 | 0.0056 | 0.005 | 0.0077 | 0.0127 | 0.0051 | 0.0061 | 0.0108 | 0.0094 | 0.0071 |
Vacuole Periphery | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.4861 | 19.7275 | 15.1808 | 16.9992 | 16.4255 | 21.239 | 16.6221 | 23.4348 | 23.8148 | 22.5959 |
Translational Efficiency | 0.9655 | 0.5259 | 0.7426 | 0.7973 | 0.8416 | 0.9662 | 1.0 | 0.8808 | 0.9968 | 0.7908 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
101 | 1080 | 1008 | 150 | 154 | 1318 | 2216 | 105 | 255 | 2398 | 3224 | 255 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1108.96 | 982.09 | 1321.35 | 1331.80 | 1024.51 | 992.75 | 1096.57 | 1205.82 | 1057.96 | 987.95 | 1166.85 | 1279.93 |
Standard Deviation | 193.52 | 192.87 | 191.57 | 268.57 | 140.87 | 146.54 | 135.21 | 180.18 | 168.89 | 169.07 | 186.81 | 244.22 |
Intensity Change Log 2 | — | -0.175280 | 0.252805 | 0.264170 | — | -0.045432 | 0.098064 | 0.235080 | — | -0.111466 | 0.180565 | 0.250274 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002939 | 0.004971 | 0.019581 | 0.008470 | 0.000699 | 0.002338 | 0.005406 | 0.023502 | 0.001587 | 0.003524 | 0.009838 | 0.014660 |
Bud Neck | 0.011147 | 0.021347 | 0.015088 | 0.018137 | 0.003378 | 0.007956 | 0.017069 | 0.022098 | 0.006455 | 0.013987 | 0.016449 | 0.019768 |
Bud Site | 0.056819 | 0.197079* | 0.159272* | 0.094901 | 0.027133 | 0.040521 | 0.059809 | 0.143636* | 0.038891 | 0.111031* | 0.090906 | 0.114968* |
Cell Periphery | 0.750960* | 0.369852* | 0.573286* | 0.750180* | 0.904697* | 0.721731* | 0.419996* | 0.490872* | 0.843805* | 0.563253* | 0.467923* | 0.643406* |
Cytoplasm | 0.161109* | 0.350328* | 0.196037* | 0.088116 | 0.028379 | 0.195726* | 0.476017* | 0.269776* | 0.080950 | 0.265355* | 0.388480* | 0.162917* |
Cytoplasmic Foci | 0.002162 | 0.016942 | 0.002667 | 0.001161 | 0.003203 | 0.005698 | 0.001573 | 0.003015 | 0.002791 | 0.010762 | 0.001915 | 0.001924 |
Eisosomes | 0.001444 | 0.000351 | 0.000935 | 0.003194 | 0.000071 | 0.000469 | 0.000846 | 0.003804 | 0.000615 | 0.000416 | 0.000874 | 0.003445 |
Endoplasmic Reticulum | 0.002166 | 0.006094 | 0.013013 | 0.010840 | 0.014480 | 0.006834 | 0.002355 | 0.007956 | 0.009603 | 0.006501 | 0.005688 | 0.009652 |
Endosome | 0.000922 | 0.007190 | 0.000790 | 0.001950 | 0.001491 | 0.002689 | 0.000624 | 0.001027 | 0.001266 | 0.004716 | 0.000676 | 0.001570 |
Golgi | 0.001777 | 0.003401 | 0.001253 | 0.003680 | 0.000599 | 0.001953 | 0.000324 | 0.001127 | 0.001065 | 0.002605 | 0.000614 | 0.002629 |
Lipid Particles | 0.000147 | 0.002654 | 0.001571 | 0.002884 | 0.000066 | 0.000434 | 0.001284 | 0.009759 | 0.000098 | 0.001434 | 0.001374 | 0.005715 |
Mitochondria | 0.000116 | 0.000690 | 0.000440 | 0.000287 | 0.001413 | 0.004581 | 0.000962 | 0.000240 | 0.000900 | 0.002829 | 0.000798 | 0.000267 |
Mitotic Spindle | 0.000435 | 0.000149 | 0.000298 | 0.000498 | 0.000212 | 0.000138 | 0.000142 | 0.000237 | 0.000300 | 0.000143 | 0.000191 | 0.000390 |
None | 0.000008 | 0.000117 | 0.000033 | 0.000026 | 0.000019 | 0.000029 | 0.000090 | 0.000020 | 0.000015 | 0.000068 | 0.000072 | 0.000023 |
Nuclear Periphery | 0.000095 | 0.000111 | 0.000379 | 0.000204 | 0.000085 | 0.000175 | 0.000174 | 0.000087 | 0.000089 | 0.000146 | 0.000238 | 0.000156 |
Nuclear Periphery Foci | 0.000422 | 0.000483 | 0.000617 | 0.000508 | 0.000299 | 0.000171 | 0.000182 | 0.000828 | 0.000348 | 0.000312 | 0.000318 | 0.000640 |
Nucleolus | 0.000007 | 0.000087 | 0.000033 | 0.000017 | 0.000012 | 0.000070 | 0.000066 | 0.000018 | 0.000010 | 0.000077 | 0.000056 | 0.000017 |
Nucleus | 0.000361 | 0.000929 | 0.001593 | 0.000837 | 0.000143 | 0.000779 | 0.001539 | 0.001522 | 0.000229 | 0.000847 | 0.001556 | 0.001119 |
Peroxisomes | 0.000248 | 0.001278 | 0.000844 | 0.000198 | 0.000119 | 0.001865 | 0.001316 | 0.000838 | 0.000170 | 0.001601 | 0.001168 | 0.000462 |
Vacuole | 0.006391 | 0.014265 | 0.011239 | 0.012864 | 0.003454 | 0.004000 | 0.009513 | 0.019339 | 0.004617 | 0.008623 | 0.010053 | 0.015530 |
Vacuole Periphery | 0.000324 | 0.001682 | 0.001031 | 0.001049 | 0.010049 | 0.001844 | 0.000713 | 0.000299 | 0.006197 | 0.001771 | 0.000812 | 0.000740 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.44 | -10.06 | -2.14 | -1.41 | 3.82 | -4.77 | -11.12 | -3.75 | -3.48 | -2.97 | -4.90 | -13.06 | -4.44 | -3.80 | -1.62 |
Bud Neck | -3.73 | -1.63 | -1.71 | 0.77 | -0.88 | -4.00 | -9.24 | -3.44 | -2.60 | -0.91 | -5.62 | -7.29 | -4.18 | -1.86 | -1.08 |
Bud Site | -11.30 | -8.40 | -2.29 | 6.58 | 4.18 | -1.78 | -4.41 | -5.96 | -5.56 | -4.55 | -10.20 | -7.97 | -6.13 | -0.41 | -2.16 |
Cell Periphery | 13.22 | 6.27 | 0.05 | -14.16 | -6.69 | 9.45 | 26.09* | 10.76 | 6.54 | -1.89 | 16.13 | 22.72 | 7.61 | -3.38 | -7.72 |
Cytoplasm | -8.44 | -1.60 | 2.97 | 16.28 | 7.06 | -20.13 | -54.39* | -9.10 | -2.77 | 7.58 | -16.59 | -27.75 | -4.75 | 6.56 | 14.48 |
Cytoplasmic Foci | -6.66 | -0.31 | 0.58 | 9.99 | 2.63 | -1.32 | 0.95 | -0.07 | 1.92 | -1.92 | -5.43 | 0.71 | 0.56 | 9.16 | -0.29 |
Eisosomes | 2.43 | 1.12 | -2.29 | -4.57 | -3.62 | -8.66 | -7.72 | -2.23 | -1.99 | -1.76 | 1.08 | -1.33 | -3.54 | -3.89 | -3.29 |
Endoplasmic Reticulum | -4.05 | -7.22 | -2.07 | -1.12 | 0.50 | 1.24 | 1.99 | 0.95 | -0.30 | -1.63 | 0.83 | 1.05 | -0.00 | -1.08 | -1.38 |
Endosome | -5.11 | 0.20 | -0.76 | 3.40 | -1.00 | -1.40 | 1.40 | 0.54 | 2.26 | -1.28 | -4.75 | 1.27 | -0.40 | 3.47 | -1.32 |
Golgi | -1.20 | 0.43 | -0.64 | -0.10 | -0.90 | -2.71 | 1.42 | -1.51 | 1.51 | -2.70 | -2.50 | 0.91 | -0.94 | -0.01 | -1.27 |
Lipid Particles | -3.01 | -7.66 | -1.40 | -0.12 | -0.68 | -4.20 | -4.45 | -1.88 | -1.81 | -1.65 | -3.53 | -6.49 | -2.33 | -1.76 | -1.80 |
Mitochondria | -4.96 | -6.06 | -1.33 | 2.43 | 1.17 | -2.29 | 0.54 | 1.41 | 3.88 | 3.71 | -2.44 | 0.20 | 1.25 | 4.12 | 3.84 |
Mitotic Spindle | 1.38 | 0.66 | -0.27 | -3.36 | -1.92 | 1.83 | 1.75 | -0.74 | -2.31 | -2.25 | 1.83 | 1.28 | -0.93 | -3.95 | -3.21 |
None | -1.60 | -9.03 | -4.09 | 1.33 | 1.63 | -0.89 | -3.53 | -0.35 | 0.45 | 3.30 | -1.71 | -4.29 | -1.42 | 1.39 | 3.64 |
Nuclear Periphery | -0.50 | -7.23 | -2.76 | -2.76 | 4.27 | -4.57 | -5.00 | -0.16 | 4.23 | 4.60 | -3.29 | -8.39 | -2.85 | -0.49 | 3.80 |
Nuclear Periphery Foci | -0.82 | -3.42 | -1.18 | -0.31 | 1.74 | 1.12 | 1.03 | -2.82 | -4.35 | -4.28 | 0.47 | 0.41 | -2.94 | -4.36 | -4.51 |
Nucleolus | -2.02 | -13.91 | -4.56 | 1.78 | 6.27 | -1.57 | -13.88 | -0.97 | 1.23 | 3.84 | -2.51 | -17.72 | -1.88 | 2.13 | 7.28 |
Nucleus | -4.10 | -8.61 | -2.49 | 0.39 | 3.30 | -1.36 | -12.50 | -2.36 | -1.21 | -0.41 | -2.32 | -15.03 | -3.22 | -0.95 | 0.95 |
Peroxisomes | -9.29 | -6.63 | 0.62 | 12.53 | 11.40 | -9.92 | -14.72 | -3.39 | 3.74 | 2.11 | -13.24 | -15.37 | -3.03 | 8.30 | 6.62 |
Vacuole | -4.67 | -4.50 | -2.37 | 0.39 | -0.68 | -0.27 | -2.97 | -3.20 | -3.35 | -2.20 | -2.79 | -4.17 | -4.01 | -2.79 | -2.32 |
Vacuole Periphery | -8.16 | -3.87 | -1.15 | 1.00 | -0.03 | 1.52 | 1.74 | 1.81 | 5.67 | 4.92 | 1.36 | 1.65 | 1.66 | 2.58 | 0.19 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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