Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 – 0 0 0 0 0.09 0.11 0.19 0 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.05 – 0.09 0.12 0.23 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.87 0.93 0.9 0.87 0.84 0.81 – 0.78 0.78 0.74 0.75 0.85 0.79 0.71 0.84 0.79 0.8 0.82 0.79 0.81 0.74 0.75 0.65
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.27 0.21 0.06 0.1 0.09 0.14 0.2 – 0.29 0.25 0.39 0.25 0.09 0.17 0.1 0.1 0.08 0.1 0.11 0.13 0.09 0.14 0.14 0.2
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1
Bud 0 0 0 0 0 0 2 – 1 0 4 0 0 0 0 0 2 1 1 3 2 0 1 0
Bud Neck 0 0 0 1 0 0 0 – 1 2 1 1 0 0 0 0 1 0 0 0 1 1 0 0
Bud Site 0 0 0 1 0 2 4 – 4 3 24 10 1 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 5 7 9 17 8 8 – 11 14 6 13 22 34 54 6 18 13 0 2 2 1 2 1
Endoplasmic Reticulum 0 1 0 1 0 0 0 – 0 0 0 0 1 0 1 1 7 2 0 0 0 0 1 0
Endosome 0 4 1 0 0 1 1 – 0 1 0 2 0 3 4 5 5 4 0 10 2 3 1 4
Golgi 0 1 0 0 0 0 1 – 0 0 0 0 0 1 1 0 1 3 0 3 0 0 0 1
Mitochondria 4 8 0 3 1 19 15 – 27 38 76 26 0 0 0 5 5 2 2 4 1 0 2 7
Nucleus 132 414 203 191 245 228 234 – 242 242 247 280 205 243 197 124 178 124 128 443 194 139 165 214
Nuclear Periphery 1 0 1 1 4 2 5 – 4 1 3 5 3 1 2 1 1 1 0 0 0 0 0 0
Nucleolus 40 101 13 22 26 39 58 – 90 78 129 92 21 53 27 15 19 15 17 75 20 25 30 66
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 3 5 1 0 2 – 7 4 1 3 5 4 8 0 1 1 2 3 1 1 2 7
Vac/Vac Membrane 1 7 4 3 6 0 1 – 2 6 1 2 1 2 5 5 13 6 4 12 8 10 12 15
Unique Cell Count 146 476 219 212 283 270 290 311 312 332 371 241 307 277 148 224 155 158 565 241 189 221 328
Labelled Cell Count 178 544 232 237 300 299 331 389 389 492 434 260 341 301 162 252 172 158 565 241 189 221 328


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 10.2 9.0 8.1 8.5 6.8 7.0 – 6.2 6.3 5.7 6.4 8.9 9.1 8.7 11.1 11.2 10.8 8.0 8.5 8.9
Std Deviation (1e-4) 1.5 2.0 1.8 1.7 1.7 1.3 1.6 – 1.3 1.5 1.3 1.6 1.7 1.9 2.3 2.1 2.6 2.5 1.6 1.4 1.4
Intensity Change (Log2) – – – -0.17 -0.09 -0.41 -0.36 – -0.55 -0.51 -0.67 -0.49 -0.02 0.01 -0.05 0.29 0.31 0.25 -0.18 -0.08 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 4.1 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 1.5 -0.1 -0.3 – 0.2 0.8 -1.1 0.2 2.6 3.3 5.5 0.4 2.2 2.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.0 3.4 – 4.5 5.4 7.6 4.0 0 0 0 0 0 0
Nucleus -1.0 -2.2 -2.8 -3.8 – -4.6 -4.7 -5.4 -5.2 -2.6 -4.3 -6.0 -2.7 -4.0 -3.6
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.7 1.3 3.0 4.5 – 6.6 5.7 8.6 5.8 1.1 3.9 1.5 1.5 1.0 1.4
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 2.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3097 2.6611 2.4263 2.3446 1.6706 2.0484 3.162 3.2879 3.078 2.9233 2.6041 2.8934 4.2071 4.3086 3.9612 3.8101 3.6789 3.7307
Actin 0.0204 0.0033 0.0049 0 0.0017 0 0.0124 0 0.0007 0 0.0017 0.0004 0.0036 0 0.0001 0 0.0001 0
Bud 0.0002 0.0001 0.0003 0 0.0001 0.0002 0.0003 0 0.0002 0 0.0002 0.0001 0.0001 0 0 0 0.0001 0
Bud Neck 0.0002 0.0002 0.0004 0.0003 0.0006 0.0028 0.0008 0 0.0009 0 0.0012 0.0007 0.0005 0.0001 0.0001 0 0.0038 0.0005
Bud Periphery 0.0003 0.0001 0.0005 0 0.0001 0.0009 0.0011 0 0.0009 0 0.0004 0.0002 0.0002 0 0 0 0.0002 0.0002
Bud Site 0.0006 0.0003 0.0012 0 0.0003 0.0005 0.0008 0 0.0006 0 0.0007 0.0001 0.0004 0 0 0 0.0002 0
Cell Periphery 0.0001 0 0.0001 0 0.0001 0.0004 0.0005 0 0.0003 0 0.0002 0 0.0001 0 0 0 0 0
Cytoplasm 0.0146 0.0001 0.0018 0 0.0017 0.0001 0.0038 0 0.0002 0 0.0001 0.0003 0.0011 0 0 0 0 0
Cytoplasmic Foci 0.008 0.0024 0.0186 0 0.0122 0 0.0061 0 0.0006 0 0.0013 0.0002 0.001 0 0 0 0 0
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0.0004 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0045 0.0003 0.0007 0 0.0022 0 0.001 0 0.0003 0 0.0001 0.0003 0.0007 0 0.0001 0 0 0
Endosome 0.0131 0.0043 0.0039 0 0.0102 0.0001 0.0045 0 0.0028 0 0.0007 0.0007 0.0018 0 0.0001 0 0.0001 0
Golgi 0.0055 0.0078 0.0014 0 0.0023 0 0.0026 0 0.0005 0 0.0012 0.0001 0.0008 0 0 0 0 0
Lipid Particles 0.0068 0.0039 0.0063 0 0.0252 0 0.0087 0 0.0016 0 0.0105 0.0003 0.0058 0 0 0 0 0
Mitochondria 0.0024 0.0176 0.0112 0.0001 0.0019 0.0002 0.0102 0 0.0062 0 0.0133 0.0007 0.0011 0 0.0003 0 0.0002 0.0017
None 0.0032 0.0001 0.0021 0 0.0023 0 0.0056 0 0.0002 0 0.0001 0.0001 0.0005 0 0 0 0 0
Nuclear Periphery 0.0214 0.0003 0.0036 0.0002 0.002 0.0002 0.0158 0.0003 0.0036 0.0002 0.001 0.0025 0.0051 0.0012 0.003 0.0002 0.0036 0.0002
Nucleolus 0.0367 0.0345 0.0349 0.0283 0.1726 0.1438 0.0362 0.028 0.026 0.0138 0.2001 0.1055 0.0347 0.0292 0.022 0.0207 0.1996 0.0954
Nucleus 0.8357 0.9225 0.8714 0.9703 0.7529 0.8489 0.8738 0.9712 0.9511 0.9858 0.7532 0.8826 0.937 0.9676 0.9711 0.978 0.7776 0.9002
Peroxisomes 0.0167 0.0014 0.0061 0 0.0014 0 0.0038 0 0.0002 0 0.0079 0.0001 0.0028 0 0 0 0 0
Punctate Nuclear 0.0071 0.0006 0.03 0.0005 0.0092 0.001 0.0051 0.0004 0.0012 0.0001 0.0054 0.0047 0.002 0.0017 0.003 0.001 0.0142 0.0011
Vacuole 0.0012 0.0002 0.0004 0 0.0004 0.0008 0.0044 0 0.0015 0 0.0004 0.0003 0.0005 0 0.0001 0 0.0002 0.0002
Vacuole Periphery 0.0012 0.0002 0.0003 0 0.0004 0.0001 0.002 0 0.0006 0 0.0003 0.0003 0.0002 0 0.0001 0 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.4341 11.9474 13.4755 14.4326 21.1403 17.0976 18.2408 17.1217 14.0444 16.6551
Translational Efficiency 1.4235 1.7191 1.2202 1.2725 0.9598 1.1548 1.0491 1.0572 1.1334 1.1208

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1287 1221 301 1303 1495 2047 784 2189 2782 3268 1085 3492

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 746.44 914.41 1176.60 1073.93 854.95 931.19 1109.37 1308.55 804.75 924.92 1128.02 1221.00
Standard Deviation 121.42 155.62 143.57 168.54 142.63 148.45 150.14 225.39 143.81 151.39 151.37 235.20
Intensity Change Log 2 — 0.292815 0.656526 0.524802 — 0.123236 0.375829 0.614057 — 0.204765 0.513482 0.573139

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000684 0.000677 0.001267 0.001294 0.000447 0.000573 0.000896 0.000428 0.000557 0.000612 0.000999 0.000751
Bud Neck 0.003631 0.006987 0.020472 0.055035 0.006250 0.008480 0.019362 0.054702 0.005039 0.007922 0.019670 0.054826
Bud Site 0.001928 0.000943 0.001537 0.005646 0.000769 0.000985 0.001101 0.002141 0.001305 0.000969 0.001222 0.003449
Cell Periphery 0.000147 0.000046 0.000031 0.000163 0.000086 0.000048 0.000038 0.000083 0.000115 0.000047 0.000036 0.000113
Cytoplasm 0.005783 0.000419 0.000065 0.007424 0.001373 0.000210 0.000270 0.002250 0.003413 0.000288 0.000213 0.004180
Cytoplasmic Foci 0.001100 0.000154 0.000053 0.002328 0.000061 0.000727 0.000017 0.000050 0.000541 0.000513 0.000027 0.000900
Eisosomes 0.000034 0.000039 0.000071 0.000033 0.000032 0.000042 0.000053 0.000027 0.000033 0.000041 0.000058 0.000029
Endoplasmic Reticulum 0.002673 0.002101 0.002032 0.004072 0.002271 0.002049 0.001999 0.002213 0.002457 0.002069 0.002009 0.002906
Endosome 0.000368 0.000388 0.000082 0.003174 0.000109 0.000571 0.000071 0.000348 0.000229 0.000502 0.000074 0.001402
Golgi 0.000429 0.000285 0.000164 0.001842 0.000099 0.000441 0.000116 0.000110 0.000251 0.000383 0.000129 0.000756
Lipid Particles 0.000113 0.000081 0.000072 0.000291 0.000031 0.000292 0.000026 0.000019 0.000069 0.000213 0.000039 0.000120
Mitochondria 0.001235 0.001070 0.001841 0.005819 0.000610 0.001203 0.001282 0.003040 0.000899 0.001153 0.001437 0.004077
Mitotic Spindle 0.000773 0.001410 0.003365 0.044390 0.000310 0.003032 0.001079 0.008192 0.000525 0.002426 0.001713 0.021699
None 0.017272 0.002507 0.000780 0.006778 0.002774 0.001077 0.001271 0.002697 0.009481 0.001611 0.001135 0.004220
Nuclear Periphery 0.000130 0.000152 0.000155 0.004807 0.000097 0.000220 0.000172 0.001383 0.000112 0.000194 0.000167 0.002661
Nuclear Periphery Foci 0.000750 0.000431 0.000198 0.002793 0.000346 0.000324 0.000072 0.000154 0.000533 0.000364 0.000107 0.001139
Nucleolus 0.105790 0.098359 0.091179 0.031835 0.086240 0.100693 0.075452 0.022770 0.095284 0.099821 0.079815 0.026153
Nucleus 0.854903* 0.881653* 0.873057* 0.815686* 0.897359* 0.877600* 0.895339* 0.897364* 0.877718* 0.879114* 0.889157* 0.866887*
Peroxisomes 0.000938 0.000860 0.002673 0.000474 0.000424 0.000656 0.000910 0.000071 0.000662 0.000732 0.001399 0.000221
Vacuole 0.000828 0.000643 0.000625 0.004226 0.000165 0.000474 0.000340 0.001560 0.000472 0.000537 0.000419 0.002555
Vacuole Periphery 0.000492 0.000796 0.000281 0.001889 0.000145 0.000303 0.000135 0.000399 0.000305 0.000487 0.000176 0.000955

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.04 -6.57 -8.39 -9.34 -2.45 -1.63 -13.57 -4.75 0.17 11.36 -0.87 -11.49 -8.09 -5.77 3.80
Bud Neck -5.47 -10.44 -22.76 -20.99 -12.56 -4.08 -12.36 -28.53 -27.14 -16.19 -7.06 -16.10 -36.63 -34.30 -20.20
Bud Site 1.70 0.11 -5.48 -7.14 -6.42 -0.53 -2.76 -5.46 -4.83 -4.88 0.99 -1.27 -7.08 -8.58 -7.97
Cell Periphery 6.05 7.12 0.52 -7.92 -10.24 1.50 1.84 0.64 -1.69 -3.23 4.25 5.11 1.22 -5.20 -8.39
Cytoplasm 4.79 5.09 0.56 -5.21 -5.60 2.55 2.28 -2.37 -8.43 -7.83 5.44 5.46 -0.18 -8.68 -8.67
Cytoplasmic Foci 2.54 2.80 0.67 -1.97 -2.27 -2.03 1.34 0.84 2.12 -1.11 0.43 2.93 1.10 0.60 -2.35
Eisosomes -1.94 -9.80 -3.69 -1.16 8.08 -5.54 -12.10 3.07 9.32 16.10 -5.30 -15.15 0.08 6.11 16.49
Endoplasmic Reticulum 2.25 2.36 -7.51 -9.34 -9.68 1.23 -0.72 -0.59 -1.87 0.22 2.59 1.03 -5.70 -8.05 -6.77
Endosome -0.02 3.04 -2.55 -2.48 -3.27 -2.94 0.07 -3.02 1.64 -3.08 -2.32 3.10 -3.04 -1.11 -3.94
Golgi 0.91 2.35 -1.75 -2.22 -3.10 -1.96 -4.43 -4.26 1.60 0.09 -0.87 1.80 -1.72 -0.34 -3.12
Lipid Particles 1.06 0.66 -3.38 -4.12 -3.70 -2.08 -1.48 0.03 2.08 1.56 -1.78 0.86 -2.61 1.15 -3.53
Mitochondria 0.44 -1.96 -6.69 -8.11 -5.95 -2.87 -13.54 -6.00 -4.29 -3.79 -1.23 -4.71 -8.42 -7.88 -6.68
Mitotic Spindle -0.96 -1.69 -8.92 -8.73 -4.95 -3.15 -2.84 -6.84 -4.87 -5.78 -3.11 -2.43 -11.14 -9.75 -8.08
None 7.31 8.45 5.74 -3.06 -5.99 4.01 3.23 -1.20 -6.39 -5.66 8.21 8.75 5.41 -6.03 -7.96
Nuclear Periphery -0.57 -1.75 -5.44 -5.41 -5.30 -1.96 -6.01 -10.28 -9.06 -9.13 -1.94 -5.47 -9.22 -8.82 -8.63
Nuclear Periphery Foci 1.43 2.51 -1.37 -2.14 -2.62 0.16 1.99 0.61 0.51 -1.59 1.23 3.52 -0.69 -1.62 -3.11
Nucleolus 1.55 0.79 16.28 14.62 8.15 -3.45 1.14 15.28 20.71 9.43 -1.42 2.17 22.61 25.34 12.11
Nucleus -4.51 -0.21 4.76 8.63 3.70 4.22 2.49 5.89 1.98 2.29 -0.47 0.44 6.51 7.18 4.76
Peroxisomes 0.94 -6.41 4.65 5.40 8.18 -3.31 -7.21 10.44 8.81 11.85 -0.97 -7.93 8.92 10.30 12.65
Vacuole 0.59 0.27 -5.90 -6.29 -6.38 -2.00 -4.25 -4.56 -3.55 -3.23 -0.36 -1.45 -7.12 -6.93 -6.74
Vacuole Periphery -1.00 1.83 -1.72 -0.24 -2.67 -2.43 -1.11 -4.47 -0.35 -4.26 -1.46 2.33 -2.47 -0.33 -3.93
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Srb4

Srb4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srb4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available