Standard name | |
---|---|
Human Ortholog | |
Description | One of three possible beta-subunits of the Snf1 kinase complex; allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; necessary and sufficient for phosphorylation of the Mig2p transcription factor in response to alkaline stress; functionally redundant with SIP1 and SIP2 for the phosphorylation of Mig1p in response to glucose deprivation; contains a glycogen-binding domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 0 | 1 | 0 | 1 | 4 | 4 | 3 | 1 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 2 |
Bud | 2 | 0 | 1 | 1 | 2 | 1 | 14 | 11 | 7 | 13 | 14 | 7 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 2 | 14 | 22 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 7 | 4 | 2 | 7 | 13 | 25 | 26 | 24 | 19 | 26 | 4 | 12 | 9 | 7 | 22 | 28 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 123 | 100 | 237 | 223 | 317 | 398 | 610 | 603 | 410 | 483 | 314 | 348 | 291 | 549 | 576 | 170 | 259 | 278 | 136 | 94 | 210 | 157 | 417 | 412 |
Endoplasmic Reticulum | 0 | 1 | 3 | 4 | 3 | 2 | 17 | 12 | 11 | 16 | 4 | 11 | 2 | 4 | 2 | 8 | 9 | 14 | 1 | 0 | 7 | 1 | 2 | 3 |
Endosome | 1 | 0 | 1 | 0 | 2 | 1 | 2 | 10 | 1 | 3 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 3 | 1 | 2 | 0 | 4 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 2 |
Mitochondria | 10 | 3 | 3 | 13 | 19 | 89 | 248 | 221 | 187 | 273 | 201 | 175 | 0 | 0 | 0 | 3 | 2 | 1 | 6 | 2 | 6 | 0 | 3 | 3 |
Nucleus | 2 | 0 | 0 | 2 | 2 | 1 | 1 | 8 | 2 | 2 | 1 | 2 | 0 | 2 | 3 | 2 | 7 | 4 | 0 | 0 | 0 | 0 | 2 | 2 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 4 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 6 | 7 |
Vac/Vac Membrane | 2 | 0 | 1 | 3 | 3 | 12 | 18 | 49 | 29 | 38 | 20 | 18 | 0 | 0 | 2 | 1 | 2 | 3 | 6 | 1 | 4 | 2 | 8 | 5 |
Unique Cell Count | 136 | 103 | 242 | 234 | 333 | 448 | 765 | 794 | 572 | 697 | 454 | 494 | 293 | 554 | 582 | 175 | 270 | 291 | 165 | 104 | 245 | 171 | 477 | 480 |
Labelled Cell Count | 144 | 107 | 253 | 253 | 350 | 516 | 933 | 951 | 679 | 855 | 581 | 596 | 297 | 570 | 594 | 191 | 304 | 331 | 165 | 104 | 245 | 171 | 477 | 480 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 7.2 | 7.4 | 7.0 | 7.0 | 6.8 | 6.6 | 6.7 | 6.7 | 6.5 | 6.3 | 6.6 | 7.8 | 7.9 | 7.6 | 13.0 | 13.2 | 13.7 | 7.5 | 7.5 | 7.6 |
Std Deviation (1e-4) | 1.8 | 2.1 | 2.2 | 2.2 | 2.2 | 2.2 | 2.0 | 2.1 | 2.0 | 2.0 | 1.9 | 1.8 | 2.2 | 2.3 | 2.2 | 2.6 | 2.3 | 2.7 | 2.4 | 2.1 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.07 | -0.12 | -0.15 | -0.14 | -0.14 | -0.17 | -0.23 | -0.15 | 0.08 | 0.1 | 0.06 | 0.82 | 0.84 | 0.9 | 0.03 | 0.04 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | 0 | 0 | 0 | 0.2 | 1.1 | 0.4 | 0.9 | 1.5 | 0 | -0.6 | 0 | 0.6 | 2.6 | 3.1 |
Cytoplasm | -1.6 | -1.7 | -4.2 | -6.7 | -7.6 | -8.5 | -9.1 | -8.9 | -8.7 | 1.4 | 1.4 | 1.2 | -0.5 | -1.3 | -1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 2.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.6 | 2.8 | 6.9 | 9.8 | 8.8 | 9.7 | 11.2 | 11.9 | 10.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.7 | 3.2 | 3.4 | 2.9 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8012 | 4.1731 | 4.0832 | 3.3101 | 3.6026 | 3.9167 | 1.0018 | 2.0696 | 1.3223 | 1.3625 | 1.5056 | 1.6688 | 0.786 | 2.0837 | 1.3351 | 1.4449 | 1.3358 | 1.3412 |
Actin | 0.0372 | 0.0004 | 0.0201 | 0.0001 | 0.0002 | 0.0002 | 0.0302 | 0.001 | 0.0087 | 0.0054 | 0.0005 | 0.008 | 0.0285 | 0.0008 | 0.0052 | 0.0014 | 0.0118 | 0.01 |
Bud | 0.0002 | 0.0003 | 0.0029 | 0.0002 | 0.0001 | 0.0001 | 0.0059 | 0.0053 | 0.0016 | 0.0005 | 0.0003 | 0.0047 | 0.0008 | 0.0006 | 0.0148 | 0.008 | 0.0004 | 0.0007 |
Bud Neck | 0.0028 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0007 | 0.005 | 0.0002 | 0.0006 | 0.001 | 0.0002 | 0.0011 | 0.0008 | 0.0001 | 0.001 | 0.0004 | 0.0008 | 0.0006 |
Bud Periphery | 0.0002 | 0.0001 | 0.0025 | 0.0001 | 0 | 0.0001 | 0.0076 | 0.0024 | 0.0031 | 0.0004 | 0.0011 | 0.0019 | 0.0008 | 0.0004 | 0.006 | 0.0036 | 0.0004 | 0.0005 |
Bud Site | 0.0012 | 0.0009 | 0.002 | 0.0001 | 0.0001 | 0 | 0.0121 | 0.0132 | 0.0068 | 0.0014 | 0.0001 | 0.0003 | 0.0019 | 0.0097 | 0.0025 | 0.0029 | 0.0008 | 0.0002 |
Cell Periphery | 0.0006 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.5453 | 0.8009 | 0.8039 | 0.7829 | 0.8363 | 0.9024 | 0.5854 | 0.8248 | 0.7519 | 0.6109 | 0.8435 | 0.8153 | 0.7088 | 0.8832 | 0.7731 | 0.8485 | 0.762 | 0.8519 |
Cytoplasmic Foci | 0.0097 | 0.0056 | 0.0149 | 0.0071 | 0.0109 | 0.0049 | 0.0625 | 0.0126 | 0.02 | 0.0621 | 0.0102 | 0.0151 | 0.0224 | 0.0059 | 0.0224 | 0.0116 | 0.0145 | 0.0132 |
Eisosomes | 0.0019 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0384 | 0.0053 | 0.0047 | 0.0022 | 0.0052 | 0.0086 | 0.008 | 0.0014 | 0.0039 | 0.0047 | 0.0025 | 0.0047 | 0.0059 | 0.0067 | 0.0035 | 0.0078 | 0.0055 | 0.0031 |
Endosome | 0.0155 | 0.0035 | 0.0135 | 0.0085 | 0.0173 | 0.0054 | 0.0667 | 0.0088 | 0.0148 | 0.06 | 0.0323 | 0.0211 | 0.0303 | 0.0083 | 0.0205 | 0.0166 | 0.0473 | 0.0114 |
Golgi | 0.0046 | 0.0005 | 0.0026 | 0.0003 | 0.0005 | 0.0004 | 0.0183 | 0.0005 | 0.0054 | 0.0062 | 0.0005 | 0.0088 | 0.0046 | 0.0012 | 0.0032 | 0.0004 | 0.0107 | 0.0023 |
Lipid Particles | 0.0072 | 0.0007 | 0.0022 | 0.0002 | 0.0001 | 0.0001 | 0.0199 | 0.0005 | 0.0055 | 0.0253 | 0.0001 | 0.0048 | 0.0078 | 0.0003 | 0.0043 | 0.0002 | 0.0062 | 0.0034 |
Mitochondria | 0.0015 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0159 | 0.0023 | 0.0071 | 0.01 | 0.0003 | 0.0017 | 0.0029 | 0.0011 | 0.0031 | 0.0004 | 0.0066 | 0.001 |
None | 0.318 | 0.1764 | 0.1212 | 0.1927 | 0.1229 | 0.0747 | 0.1075 | 0.1209 | 0.1524 | 0.1718 | 0.0958 | 0.1042 | 0.1523 | 0.0764 | 0.1254 | 0.0857 | 0.1094 | 0.0956 |
Nuclear Periphery | 0.0048 | 0.0007 | 0.0017 | 0.0006 | 0.0005 | 0.0004 | 0.0038 | 0.0004 | 0.0015 | 0.0031 | 0.001 | 0.001 | 0.0124 | 0.0013 | 0.0019 | 0.002 | 0.0082 | 0.0009 |
Nucleolus | 0.0006 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0008 | 0.0009 | 0.0005 | 0.0012 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 |
Nucleus | 0.0019 | 0.0006 | 0.0008 | 0.0008 | 0.0005 | 0.0004 | 0.002 | 0.0008 | 0.0015 | 0.0018 | 0.0008 | 0.0007 | 0.0042 | 0.0009 | 0.0027 | 0.0017 | 0.0024 | 0.0009 |
Peroxisomes | 0.0011 | 0 | 0.0011 | 0 | 0 | 0 | 0.0306 | 0.0004 | 0.0048 | 0.0177 | 0 | 0.0036 | 0.0048 | 0.0002 | 0.0027 | 0.0002 | 0.0016 | 0.0007 |
Punctate Nuclear | 0.0039 | 0.0002 | 0.001 | 0.0002 | 0.0001 | 0.0001 | 0.01 | 0.0005 | 0.0018 | 0.0048 | 0.0001 | 0.0005 | 0.0048 | 0.0003 | 0.001 | 0.0006 | 0.0055 | 0.0008 |
Vacuole | 0.0028 | 0.0027 | 0.003 | 0.0035 | 0.0039 | 0.0013 | 0.0057 | 0.0022 | 0.0054 | 0.0088 | 0.0082 | 0.0017 | 0.0039 | 0.002 | 0.0053 | 0.007 | 0.0036 | 0.0021 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0005 | 0.0005 | 0.0007 | 0.0002 | 0.0014 | 0.0004 | 0.0023 | 0.0027 | 0.0022 | 0.0006 | 0.0012 | 0.0004 | 0.001 | 0.001 | 0.0017 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.2748 | 44.0487 | 23.3472 | 35.6129 | 33.6548 | 33.418 | 34.1985 | 29.5231 | 31.9424 | 38.1259 |
Translational Efficiency | 1.9912 | 1.5864 | 2.6136 | 1.5969 | 2.475 | 3.1939 | 1.8669 | 2.2547 | 2.3372 | 1.965 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
627 | 582 | 2624 | 932 | 1481 | 1458 | 451 | 1758 | 2108 | 2040 | 3075 | 2690 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 718.68 | 776.62 | 1060.49 | 1184.05 | 675.35 | 741.94 | 1241.65 | 1155.09 | 688.24 | 751.83 | 1087.06 | 1165.12 |
Standard Deviation | 126.40 | 170.88 | 178.90 | 226.93 | 126.26 | 141.18 | 213.87 | 246.00 | 127.85 | 151.07 | 195.26 | 239.96 |
Intensity Change Log 2 | — | 0.111859 | 0.561310 | 0.720309 | — | 0.135667 | 0.878551 | 0.774298 | — | 0.123442 | 0.723714 | 0.746717 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000458 | 0.000440 | 0.000276 | 0.001426 | 0.000229 | 0.000664 | 0.000574 | 0.000802 | 0.000298 | 0.000600 | 0.000320 | 0.001019 |
Bud Neck | 0.019409 | 0.025006 | 0.001288 | 0.002114 | 0.024572 | 0.025264 | 0.001106 | 0.001745 | 0.023037 | 0.025191 | 0.001262 | 0.001873 |
Bud Site | 0.025464 | 0.034499 | 0.010147 | 0.032250 | 0.047637 | 0.032993 | 0.013877 | 0.019858 | 0.041042 | 0.033423 | 0.010694 | 0.024152 |
Cell Periphery | 0.000304 | 0.000155 | 0.000060 | 0.000355 | 0.001704 | 0.000167 | 0.000113 | 0.000147 | 0.001288 | 0.000164 | 0.000068 | 0.000219 |
Cytoplasm | 0.502966* | 0.467851* | 0.830344* | 0.760873* | 0.636596* | 0.531174* | 0.819539* | 0.849903* | 0.596849* | 0.513108* | 0.828759* | 0.819057* |
Cytoplasmic Foci | 0.298691* | 0.275903* | 0.029799 | 0.040681 | 0.098011 | 0.246828* | 0.057676 | 0.027359 | 0.157701 | 0.255123* | 0.033888 | 0.031974 |
Eisosomes | 0.000254 | 0.000121 | 0.000005 | 0.000019 | 0.000150 | 0.000134 | 0.000008 | 0.000007 | 0.000181 | 0.000130 | 0.000006 | 0.000011 |
Endoplasmic Reticulum | 0.003357 | 0.000635 | 0.000212 | 0.000980 | 0.003180 | 0.000950 | 0.000497 | 0.000146 | 0.003233 | 0.000860 | 0.000254 | 0.000435 |
Endosome | 0.025722 | 0.049979 | 0.002761 | 0.005522 | 0.012320 | 0.029173 | 0.003708 | 0.002867 | 0.016306 | 0.035109 | 0.002900 | 0.003787 |
Golgi | 0.011582 | 0.011683 | 0.000404 | 0.004082 | 0.002318 | 0.012353 | 0.001517 | 0.003748 | 0.005073 | 0.012162 | 0.000567 | 0.003864 |
Lipid Particles | 0.008678 | 0.005026 | 0.000569 | 0.001158 | 0.002921 | 0.006004 | 0.001075 | 0.000735 | 0.004633 | 0.005725 | 0.000643 | 0.000882 |
Mitochondria | 0.007381 | 0.005681 | 0.000130 | 0.002001 | 0.002381 | 0.011494 | 0.000871 | 0.001430 | 0.003868 | 0.009836 | 0.000239 | 0.001628 |
Mitotic Spindle | 0.001441 | 0.003065 | 0.001631 | 0.011441 | 0.001197 | 0.004847 | 0.000519 | 0.008072 | 0.001269 | 0.004338 | 0.001468 | 0.009239 |
None | 0.001336 | 0.002912 | 0.001016 | 0.000425 | 0.015124 | 0.002349 | 0.001560 | 0.000720 | 0.011023 | 0.002509 | 0.001095 | 0.000618 |
Nuclear Periphery | 0.000201 | 0.000148 | 0.000048 | 0.000203 | 0.000173 | 0.000105 | 0.000068 | 0.000060 | 0.000181 | 0.000117 | 0.000051 | 0.000110 |
Nuclear Periphery Foci | 0.000717 | 0.000912 | 0.000482 | 0.001065 | 0.001297 | 0.000396 | 0.004139 | 0.000655 | 0.001125 | 0.000543 | 0.001018 | 0.000797 |
Nucleolus | 0.000567 | 0.001576 | 0.000155 | 0.000131 | 0.000878 | 0.000669 | 0.000069 | 0.000126 | 0.000786 | 0.000928 | 0.000143 | 0.000128 |
Nucleus | 0.029557 | 0.021463 | 0.020571 | 0.012159 | 0.043562 | 0.019539 | 0.016199 | 0.013163 | 0.039397 | 0.020088 | 0.019930 | 0.012815 |
Peroxisomes | 0.006680 | 0.016682 | 0.000385 | 0.001078 | 0.001353 | 0.011219 | 0.000442 | 0.001375 | 0.002937 | 0.012778 | 0.000394 | 0.001272 |
Vacuole | 0.050984 | 0.072318 | 0.099046 | 0.117984 | 0.101554 | 0.060277 | 0.075887 | 0.065803 | 0.086513 | 0.063712 | 0.095650 | 0.083882 |
Vacuole Periphery | 0.004251 | 0.003945 | 0.000670 | 0.004053 | 0.002843 | 0.003402 | 0.000556 | 0.001279 | 0.003262 | 0.003557 | 0.000654 | 0.002240 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.11 | 1.07 | -2.35 | -2.46 | -2.82 | -2.10 | -1.51 | -1.54 | 0.07 | -0.39 | -1.98 | 0.01 | -2.63 | -0.94 | -2.23 |
Bud Neck | -1.55 | 8.15 | 7.80 | 8.39 | -3.82 | -0.28 | 13.69 | 13.39 | 13.30 | -3.00 | -1.04 | 15.88 | 15.47 | 15.71 | -4.07 |
Bud Site | -2.21 | 5.28 | -0.75 | 1.57 | -6.96 | 4.69 | 11.64 | 10.87 | 7.23 | -1.76 | 3.08 | 14.51 | 8.69 | 6.39 | -7.45 |
Cell Periphery | 4.51 | 7.94 | 0.09 | -2.24 | -3.85 | 3.99 | 4.14 | 4.11 | 3.89 | -1.13 | 4.15 | 4.52 | 4.06 | -0.83 | -5.27 |
Cytoplasm | 2.14 | -26.65 | -17.16 | -17.92 | 8.23 | 10.31 | -13.00 | -21.09 | -29.65 | -1.77 | 9.44 | -31.93 | -26.03 | -33.79 | 4.50 |
Cytoplasmic Foci | 1.77 | 30.56 | 28.89 | 25.18 | -3.04 | -22.25 | 8.00 | 19.15 | 37.33 | 7.89 | -15.43 | 28.87 | 29.82 | 44.73 | 2.38 |
Eisosomes | 3.66 | 7.15 | 6.71 | 9.38 | -2.10 | 0.93 | 12.11 | 12.37 | 10.43 | 1.28 | 3.13 | 13.31 | 12.75 | 12.87 | -2.63 |
Endoplasmic Reticulum | 2.10 | 2.45 | 1.94 | -0.55 | -2.20 | 4.21 | 5.32 | 6.02 | 4.05 | 2.62 | 4.43 | 5.76 | 5.38 | 2.78 | -1.54 |
Endosome | -5.91 | 12.03 | 10.39 | 12.26 | -2.71 | -9.53 | 9.35 | 10.96 | 17.15 | 2.37 | -10.78 | 15.78 | 14.72 | 20.56 | -0.94 |
Golgi | -0.03 | 10.31 | 6.10 | 4.29 | -3.30 | -9.27 | 1.99 | -0.47 | 7.83 | -1.73 | -7.18 | 12.36 | 3.62 | 8.91 | -4.51 |
Lipid Particles | 2.76 | 8.12 | 7.46 | 4.60 | -3.96 | -2.99 | 4.74 | 5.73 | 5.37 | 1.57 | -1.33 | 9.95 | 9.25 | 6.56 | -2.91 |
Mitochondria | 0.83 | 4.09 | 3.04 | 3.48 | -1.96 | -5.79 | 1.98 | 1.66 | 6.77 | -0.47 | -4.69 | 5.33 | 3.29 | 7.47 | -2.30 |
Mitotic Spindle | -1.09 | -0.03 | -3.32 | -2.89 | -3.62 | -3.09 | 0.99 | -3.60 | -0.97 | -4.43 | -3.24 | -0.15 | -4.93 | -2.47 | -5.08 |
None | -1.42 | 1.06 | 2.64 | 2.33 | 4.21 | 6.49 | 6.86 | 7.41 | 4.98 | 1.22 | 5.95 | 7.26 | 7.56 | 4.88 | 3.19 |
Nuclear Periphery | 1.11 | 3.85 | 0.19 | -0.57 | -2.10 | 1.60 | 1.67 | 1.31 | -0.64 | -0.79 | 1.95 | 3.93 | 1.03 | -0.91 | -3.34 |
Nuclear Periphery Foci | -0.87 | 1.10 | -1.97 | -0.91 | -4.41 | 2.27 | -5.65 | 1.32 | -4.86 | 10.00 | 2.00 | 0.17 | 0.80 | -4.01 | 2.06 |
Nucleolus | -2.51 | 3.81 | 4.06 | 3.69 | 2.34 | 1.89 | 10.12 | 9.42 | 7.26 | -6.33 | -1.10 | 9.92 | 10.18 | 6.38 | 2.25 |
Nucleus | 1.91 | 2.12 | 4.45 | 2.90 | 4.94 | 10.75 | 8.97 | 8.82 | -1.36 | -0.91 | 9.79 | 9.07 | 10.13 | 0.59 | 2.00 |
Peroxisomes | -4.50 | 5.73 | 5.16 | 8.05 | -2.98 | -8.99 | 6.29 | 1.10 | 9.11 | -2.74 | -9.68 | 7.40 | 5.07 | 12.10 | -3.92 |
Vacuole | -3.94 | -13.25 | -13.26 | -8.97 | -5.18 | 8.82 | 0.25 | 0.09 | -9.14 | -0.19 | 6.08 | -4.88 | -6.68 | -13.05 | -2.24 |
Vacuole Periphery | 0.43 | 6.05 | 0.94 | 0.68 | -2.50 | -1.19 | 6.02 | 4.74 | 6.72 | -1.71 | -0.70 | 8.71 | 3.24 | 4.03 | -2.92 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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