Standard name
Human Ortholog
Description Mitochondrial copper and phosphate carrier; imports copper and inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Bud 0 0 0.06 0 0 0.06 0.05 0 0 0 0.05 0 0 0 0 – 0.09 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Cytoplasm 0.09 0.09 0.12 0.09 0.09 0.06 0 0.05 0 0.07 0 0 0.12 0.11 0.14 – 0.44 0.47 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0 – 0.12 0.16 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0.19 – 0.21 0.05 0 0.05 0.1 0.06 0.1 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.12 0.07 – 0 0.05 0.07 0 0 0 0 0
Mitochondria 0.89 0.87 0.83 0.88 0.9 0.91 0.93 0.9 0.95 0.91 0.95 0.97 0.38 0.43 0.35 – 0.14 0.11 0.79 0.76 0.72 0.72 0.65 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0.06 0.07 0.07 0 0 0 – 0 0.05 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.09 0 0 0 0 0 0 0 0 0 0.1 0 0.1 – 0 0.05 0 0 0.05 0 0.05 0.06
SpindlePole 0 0 0.08 0 0 0 0.05 0.05 0 0 0 0 0 0 0 – 0.12 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0.08 0 0 0 0 0 0 0 0.31 0.22 0.34 – 0 0.05 0 0 0 0.05 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 1 1 0 0 0 0 0 0 0 0 3 4 1 – 1 1 1 3 3 4 4 9
Bud 0 0 18 5 6 19 23 15 19 16 27 21 0 1 1 – 4 1 0 0 0 1 1 4
Bud Neck 0 0 2 1 0 1 3 0 0 0 0 0 0 0 0 – 0 0 0 1 0 1 2 6
Bud Site 0 0 6 0 0 6 12 3 8 6 8 13 0 0 0 – 0 1 – – – – – –
Cell Periphery 0 4 5 1 1 0 1 1 2 1 4 3 4 2 5 – 1 1 0 0 0 0 0 0
Cytoplasm 6 10 36 19 26 20 21 21 13 29 10 10 16 16 23 – 19 9 2 1 7 0 0 1
Endoplasmic Reticulum 0 7 0 3 2 7 1 1 4 3 3 1 12 18 3 – 5 3 0 1 2 1 1 5
Endosome 0 1 4 2 4 0 0 4 0 0 1 0 13 13 31 – 9 1 3 6 31 9 17 20
Golgi 2 0 0 0 1 0 0 0 0 0 0 0 9 17 12 – 0 1 5 2 3 3 6 9
Mitochondria 62 95 245 197 257 300 443 360 413 391 469 567 51 63 57 – 6 2 66 100 228 122 118 166
Nucleus 1 1 5 0 4 9 15 18 12 10 6 7 2 1 0 – 1 1 0 0 0 0 1 0
Nuclear Periphery 0 0 2 0 2 14 13 14 12 25 33 39 0 2 0 – 0 1 0 1 1 2 0 2
Nucleolus 4 0 1 0 2 4 12 19 18 12 29 20 0 0 2 – 0 0 0 0 1 0 0 1
Peroxisomes 0 1 27 1 5 0 5 4 0 1 1 2 13 2 16 – 1 1 2 4 15 7 9 16
SpindlePole 1 1 24 9 8 11 22 19 19 13 18 13 3 5 3 – 5 2 0 0 6 3 2 4
Vac/Vac Membrane 1 6 9 4 22 9 18 14 13 16 19 14 42 32 55 – 1 1 0 5 6 8 13 22
Unique Cell Count 70 109 294 223 286 329 474 400 433 429 496 582 134 145 163 43 19 85 133 317 169 184 277
Labelled Cell Count 77 131 385 243 340 400 589 493 533 523 628 710 168 176 209 53 26 85 133 317 169 184 277


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 4.5 3.6 3.5 3.6 3.7 3.3 3.6 3.3 3.2 3.6 3.7 6.6 6.1 6.4 4.9 5.0 5.4 6.0 6.5 6.7
Std Deviation (1e-4) 1.5 0.8 1.1 0.7 0.8 1.2 0.9 1.0 0.8 1.0 1.2 1.2 6.8 1.0 1.1 0.9 1.3 1.0 1.1 1.9 1.9
Intensity Change (Log2) – – – -0.06 0.0 0.01 -0.15 0.0 -0.14 -0.2 -0.02 0.01 0.87 0.75 0.82 0.42 0.45 0.58 0.72 0.85 0.89


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8488 3.4977 3.7749 3.5349 3.4641 3.5663 7.5098 7.0718 7.0048 7.4837 5.581 6.758 6.4764 5.218 5.7839 4.7795 4.3305 5.1593
Actin 0.0126 0.0054 0.0117 0.0104 0.0015 0.0054 0.023 0.0092 0.0041 0.0008 0.0059 0.0088 0.0033 0.0069 0.0039 0.0011 0.0005 0.0042
Bud 0.0005 0.0014 0.0006 0.0027 0.0016 0.0002 0.0007 0.0009 0.0003 0.0001 0.0003 0.0002 0.0003 0.0017 0.0002 0.0006 0.0001 0.0003
Bud Neck 0.0032 0.0005 0.0024 0.0001 0.0001 0.0006 0.0005 0.0003 0.0001 0 0.0002 0.0004 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001
Bud Periphery 0.001 0.003 0.0012 0.0066 0.0047 0.0008 0.0017 0.0018 0.001 0.0004 0.0006 0.0005 0.0008 0.0026 0.001 0.0027 0.0004 0.0007
Bud Site 0.0019 0.008 0.003 0.0008 0.0006 0.0007 0.0027 0.0091 0.0009 0.0001 0.0004 0.0002 0.0009 0.0029 0.0005 0.0002 0.0001 0.0002
Cell Periphery 0.0004 0.0005 0.0007 0.0002 0.0001 0.0001 0.0007 0.0005 0.0005 0.0001 0.0001 0.0002 0.0004 0.0005 0.0003 0.0004 0.0001 0.0002
Cytoplasm 0.0009 0.001 0.0006 0.0017 0.0004 0.0002 0.004 0.0007 0.0006 0 0.0101 0.0006 0.0002 0.0004 0.0001 0.0004 0.0002 0.0001
Cytoplasmic Foci 0.0226 0.02 0.0049 0.0078 0.009 0.0042 0.0132 0.0138 0.0043 0.0002 0.0088 0.0034 0.0052 0.0065 0.0031 0.0022 0.0223 0.0029
Eisosomes 0.0002 0.0002 0.0003 0.0002 0.0001 0.0002 0.0013 0.0003 0.0003 0.0001 0.0001 0.0002 0.0003 0.0001 0.0006 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0012 0.001 0.0013 0.0003 0.0007 0.0003 0.0057 0.0005 0.0024 0.0001 0.0012 0.0012 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002
Endosome 0.0638 0.0374 0.0276 0.0372 0.0253 0.0132 0.0356 0.0121 0.0069 0.0013 0.0152 0.0083 0.0177 0.0145 0.0084 0.0093 0.0276 0.0108
Golgi 0.0348 0.0324 0.0217 0.031 0.0184 0.0194 0.0278 0.0204 0.007 0.0012 0.0076 0.0133 0.0219 0.0244 0.011 0.0138 0.0257 0.0129
Lipid Particles 0.0354 0.009 0.0107 0.0118 0.0075 0.0088 0.014 0.0036 0.0041 0.0003 0.0111 0.0033 0.0216 0.003 0.0027 0.0015 0.0105 0.0042
Mitochondria 0.7644 0.8327 0.8928 0.872 0.8853 0.9306 0.806 0.8785 0.9397 0.9928 0.8656 0.9385 0.8865 0.8878 0.9526 0.9622 0.8897 0.9458
None 0.0012 0.0004 0.0003 0.0012 0.0002 0.0002 0.0034 0.0028 0.0004 0.0001 0.0018 0.0005 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001
Nuclear Periphery 0.0023 0.0025 0.0022 0.0002 0.001 0.001 0.0123 0.0068 0.0014 0.0001 0.0075 0.0003 0.0009 0.0001 0.0002 0.0001 0 0.0003
Nucleolus 0.0035 0.0004 0.0005 0.0001 0.0001 0.0001 0.0005 0.0015 0.0002 0 0.0004 0.0001 0.0011 0.0006 0.0001 0.0001 0.0001 0.0003
Nucleus 0.0007 0.0005 0.0004 0.0001 0.0002 0.0001 0.0019 0.001 0.0002 0 0.0202 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001
Peroxisomes 0.0311 0.0252 0.007 0.0116 0.0358 0.0094 0.0152 0.0171 0.0144 0.0003 0.0294 0.0159 0.0226 0.0193 0.0073 0.0019 0.0168 0.0108
Punctate Nuclear 0.0031 0.0002 0.0004 0.0002 0.0001 0.0003 0.0018 0.008 0.0001 0 0.011 0.0001 0.0002 0 0 0 0 0
Vacuole 0.0084 0.0088 0.0041 0.0015 0.0034 0.001 0.0138 0.003 0.0056 0.0006 0.0012 0.0011 0.006 0.0166 0.0029 0.0012 0.002 0.0017
Vacuole Periphery 0.0067 0.0096 0.0057 0.0024 0.0041 0.0033 0.0141 0.0079 0.0055 0.0014 0.0012 0.0029 0.0088 0.0115 0.0045 0.0018 0.0033 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5535 8.7387 12.1584 18.443 14.2586 10.9913 11.8729 14.9087 18.0936 15.7213
Translational Efficiency 1.2337 1.0501 0.7078 0.7602 0.6247 1.1272 0.7394 0.6229 0.9725 0.5114

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1467 1945 377 1464 1689 1942 204 1872 3156 3887 581 3336

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 687.12 781.63 1377.08 855.68 707.78 733.14 877.51 902.29 698.18 757.40 1201.67 881.84
Standard Deviation 78.37 107.85 394.40 185.00 91.87 96.58 108.26 150.34 86.48 105.21 402.38 168.04
Intensity Change Log 2 — 0.185924 1.002978 0.316509 — 0.050788 0.310115 0.350290 — 0.118937 0.692703 0.333749

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000594 0.001298 0.001615 0.008637 0.000563 0.000903 0.003619 0.020801 0.000577 0.001101 0.002318 0.015463
Bud Neck 0.018261 0.008832 0.007631 0.017215 0.016945 0.005234 0.027932 0.010961 0.017557 0.007034 0.014759 0.013705
Bud Site 0.016863 0.015849 0.012573 0.108087* 0.015559 0.010932 0.085085 0.134583* 0.016165 0.013392 0.038033 0.122955*
Cell Periphery 0.000182 0.000310 0.000331 0.001044 0.000291 0.000301 0.000504 0.001746 0.000240 0.000305 0.000392 0.001438
Cytoplasm 0.031122 0.007723 0.003952 0.048191 0.024962 0.006223 0.021318 0.042666 0.027825 0.006974 0.010050 0.045090
Cytoplasmic Foci 0.126899 0.065640 0.068105 0.076158 0.112431 0.056395 0.075655 0.052056 0.119156 0.061021 0.070756 0.062633
Eisosomes 0.000148 0.000106 0.000104 0.000143 0.000152 0.000101 0.000136 0.000202 0.000150 0.000103 0.000116 0.000176
Endoplasmic Reticulum 0.001190 0.000455 0.000785 0.002049 0.001310 0.000262 0.005501 0.001598 0.001254 0.000359 0.002441 0.001796
Endosome 0.071279 0.089208 0.050990 0.062625 0.089364 0.080792 0.133832 0.047816 0.080957 0.085003 0.080077 0.054315
Golgi 0.086961 0.138744* 0.077326 0.100531 0.100757 0.153491* 0.050254 0.078097 0.094344 0.146112* 0.067821 0.087942
Lipid Particles 0.008621 0.002674 0.005593 0.003145 0.010026 0.003372 0.003263 0.000738 0.009373 0.003023 0.004775 0.001794
Mitochondria 0.553624* 0.583984* 0.535183* 0.343619* 0.540106* 0.614075* 0.290642* 0.459550* 0.546390* 0.599018* 0.449320* 0.408674*
Mitotic Spindle 0.007811 0.013425 0.053819 0.077554 0.007491 0.007646 0.074470 0.035153 0.007640 0.010538 0.061070 0.053761
None 0.000625 0.000560 0.001773 0.003497 0.000962 0.000403 0.000537 0.000505 0.000805 0.000481 0.001339 0.001818
Nuclear Periphery 0.000167 0.000078 0.005646 0.000399 0.000198 0.000145 0.000846 0.000252 0.000184 0.000111 0.003960 0.000317
Nuclear Periphery Foci 0.000101 0.000113 0.003600 0.001180 0.000174 0.000180 0.001613 0.000303 0.000140 0.000146 0.002902 0.000688
Nucleolus 0.000361 0.000498 0.001961 0.001624 0.001664 0.000570 0.003206 0.000295 0.001059 0.000534 0.002398 0.000878
Nucleus 0.003615 0.001225 0.000715 0.007207 0.004098 0.000945 0.020240 0.002736 0.003874 0.001086 0.007570 0.004698
Peroxisomes 0.021618 0.033758 0.011924 0.046332 0.021675 0.029014 0.098007 0.050093 0.021649 0.031388 0.042149 0.048442
Vacuole 0.033216 0.020031 0.064996 0.068530 0.029685 0.014471 0.074473 0.043137 0.031326 0.017254 0.068323 0.054281
Vacuole Periphery 0.016743 0.015489 0.091380 0.022234 0.021587 0.014544 0.028867 0.016714 0.019335 0.015017 0.069431 0.019137

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.71 -3.14 -8.63 -7.68 -7.18 -2.03 -4.58 -10.26 -10.08 -8.20 -3.38 -5.50 -12.57 -12.08 -10.72
Bud Neck 5.24 5.68 0.41 -4.57 -5.03 7.23 -2.59 3.54 -6.47 4.26 8.69 1.51 2.78 -6.96 0.50
Bud Site 0.51 1.64 -18.89 -19.29 -19.12 2.93 -6.82 -24.91 -26.13* -4.52 2.22 -5.36 -31.17 -32.27 -16.38
Cell Periphery -2.37 -1.96 -3.01 -2.57 -2.44 -0.27 -2.43 -6.73 -6.69 -5.45 -2.03 -2.69 -6.87 -6.51 -5.82
Cytoplasm 10.07 9.73 -5.39 -14.25 -13.82 9.58 0.60 -6.40 -15.84 -4.56 13.87 7.47 -8.25 -21.25 -14.15
Cytoplasmic Foci 13.48 7.69 10.43 -2.92 -1.14 13.93 4.85 14.91 1.39 3.52 19.25 8.78 18.05 -0.75 1.62
Eisosomes 3.73 2.83 0.36 -3.41 -2.56 5.06 1.05 -3.80 -8.32 -4.42 6.23 3.20 -2.72 -8.77 -5.38
Endoplasmic Reticulum 2.84 1.44 -2.46 -4.73 -3.67 3.93 -2.51 -1.12 -9.46 2.35 4.75 -1.98 -2.57 -8.35 1.07
Endosome -3.99 3.30 2.29 6.63 -1.85 1.94 -3.60 10.67 9.37 7.34 -1.29 0.27 9.57 11.53 4.48
Golgi -9.46 1.23 -2.06 6.82 -2.56 -9.17 6.77 4.82 14.03 -3.87 -13.01 4.63 2.09 14.99 -3.34
Lipid Particles 5.69 2.35 4.41 -0.75 2.01 5.25 4.66 8.40 3.88 3.46 7.66 4.61 9.29 2.23 4.22
Mitochondria -2.62 0.99 17.65 21.02 9.40 -6.79 10.89 7.17 14.10 -7.35 -6.65 6.13 16.74 23.85 2.50
Mitotic Spindle -3.85 -6.43 -16.38 -14.93 -2.85 -0.13 -7.67 -11.93 -12.58 4.42 -3.06 -9.59 -20.02 -18.89 1.27
None 0.29 -4.60 -2.52 -2.58 -1.54 2.38 1.85 2.13 -0.71 0.44 1.86 -2.71 -1.92 -2.63 -0.96
Nuclear Periphery 1.69 -5.99 -2.29 -3.24 5.68 0.75 -2.77 -0.39 -0.72 2.21 1.63 -6.24 -1.51 -2.24 5.94
Nuclear Periphery Foci -0.75 -7.18 -3.39 -3.35 3.80 -0.28 -3.76 -1.12 -0.84 3.36 -0.38 -8.03 -3.57 -3.43 5.69
Nucleolus -0.88 -4.01 -3.50 -2.95 0.42 2.63 -1.64 3.42 1.27 3.45 2.28 -2.88 0.70 -1.77 3.47
Nucleus 3.96 5.05 -3.17 -4.85 -5.23 4.93 -2.96 1.28 -3.64 3.12 6.29 -2.05 -1.95 -5.95 1.34
Peroxisomes -4.42 3.83 -6.87 -3.33 -9.56 -2.93 -6.25 -8.98 -6.20 3.89 -5.26 -4.23 -11.26 -6.75 -1.14
Vacuole 5.71 -6.00 -9.85 -13.95 -1.15 7.46 -6.33 -5.12 -11.58 4.22 9.16 -8.79 -10.61 -17.82 2.71
Vacuole Periphery 1.00 -10.31 -2.77 -3.50 9.45 5.33 -1.66 3.27 -1.45 2.87 4.70 -9.86 0.35 -3.45 9.86
Standard name
Human Ortholog
Description Mitochondrial copper and phosphate carrier; imports copper and inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Pic2

Pic2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pic2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available