Standard name
Human Ortholog
Description Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion or in response to DNA replication stress; RGI1 has a paralog, RGI2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.96 0.88 0.76 0.7 0.61 0.72 0.71 0.75 0.77 0.99 0.97 0.97 0.94 0.95 0.96 0.93 0.83 0.86 0.85 0.8 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.19 0.17 0.11 0.13 0.12 0.19 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0.13 0.16 0.28 0.18 0.19 0.1 0.13 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 0 2 3 1 1 0 2 0 0 0 0 0 1 0 2 5 1 2 3
Bud 1 1 1 2 8 11 25 16 16 14 10 11 0 0 0 0 0 1 2 31 34 9 21 12
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 3 1 3 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 4 1 6 9 5 9 15 10 21 0 1 1 2 1 5 0 1 1 0 2 1
Cytoplasm 155 388 668 433 442 649 649 538 446 520 375 482 151 229 231 127 116 128 150 334 563 231 308 253
Endoplasmic Reticulum 0 1 2 0 0 2 2 8 7 10 2 2 0 2 0 4 4 3 0 0 3 1 2 0
Endosome 0 1 5 4 1 1 3 1 0 2 1 4 0 2 1 1 0 0 0 3 5 0 2 2
Golgi 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 1 0 0 1
Mitochondria 2 5 6 17 30 166 157 95 80 86 94 77 1 0 0 3 1 3 3 3 6 9 7 8
Nucleus 0 0 0 4 7 18 27 40 14 18 10 17 0 0 1 1 2 2 0 4 1 2 5 1
Nuclear Periphery 0 0 0 0 2 16 20 23 6 16 5 7 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 2 0 0 1 1 0 0 0 2 0 0 0 1 1 7 15 5 11 4
Vac/Vac Membrane 1 4 8 10 46 111 144 248 109 137 52 82 3 3 7 3 7 1 0 4 1 2 8 6
Unique Cell Count 158 396 680 451 501 853 922 881 617 734 497 630 153 236 237 135 122 133 162 401 655 272 386 304
Labelled Cell Count 160 401 694 474 541 984 1038 979 690 820 559 705 155 240 241 141 132 145 162 401 655 272 386 304


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 6.9 5.7 5.7 8.5 8.3 9.2 9.5 10.5 10.2 9.5 10.2 6.0 8.8 10.6 8.7 8.8 8.4 5.3 5.9 6.0
Std Deviation (1e-4) 1.5 4.0 2.5 2.5 5.3 5.2 5.5 5.0 5.6 5.5 5.2 5.7 1.8 5.3 8.8 3.0 2.5 2.1 1.9 2.9 2.8
Intensity Change (Log2) – – – -0.02 0.56 0.53 0.67 0.73 0.87 0.83 0.73 0.83 0.06 0.61 0.88 0.59 0.61 0.54 -0.11 0.03 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 2.5 4.0 3.2 3.9 3.2 0 3.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.3 3.0 2.9 4.2 0 0 0 0 0 0
Cytoplasm -2.3 -7.2 -12.4 -14.4 -17.4 -13.4 -14.0 -12.2 -12.0 0.4 -1.1 -0.7 -2.9 -2.2 -1.5
Endoplasmic Reticulum 0 0 0 0 0 0 2.2 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.4 5.0 11.5 10.6 7.9 8.7 8.3 11.0 8.4 0 0 0 0 0 0
Nucleus 0 0 3.8 4.5 5.6 3.9 4.1 0 4.3 0 0 0 0 0 0
Nuclear Periphery 0 0 3.6 3.9 4.2 0 3.9 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.4 6.5 8.6 9.7 14.3 10.4 10.8 7.2 8.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.3909 10.0643 9.0066 7.9115 6.2019 10.3435 11.5784 7.6351 8.2992 6.9725 6.6053 9.4552 8.0283 6.7266 9.9822 9.6857 5.4722 11.8097
Actin 0.0018 0.0044 0.0067 0.0058 0.0054 0.0025 0.0173 0.0037 0.0121 0.0032 0.0344 0.0022 0.003 0.0003 0.0019 0.0008 0.0035 0.0002
Bud 0.0018 0.0269 0.018 0.0384 0.0047 0.0045 0.0045 0.0251 0.0114 0.0101 0.0098 0.0023 0.0007 0.0007 0.0005 0.0001 0.0076 0.0001
Bud Neck 0.0005 0.0008 0.001 0.0016 0.0026 0.0028 0.0017 0.0005 0.0023 0.0013 0.0041 0.0028 0.0002 0.0001 0.0002 0.0004 0.0005 0.001
Bud Periphery 0.0011 0.0071 0.0085 0.0151 0.003 0.003 0.0066 0.0133 0.0063 0.0062 0.0077 0.0034 0.0004 0.0003 0.0001 0 0.0038 0
Bud Site 0.0014 0.0237 0.014 0.015 0.0014 0.0007 0.0057 0.01 0.0079 0.0007 0.0116 0.0004 0.0018 0.0003 0.0004 0.0001 0.0003 0
Cell Periphery 0.0002 0.0005 0.0004 0.0007 0.0003 0.0002 0.0003 0.0004 0.0004 0.0001 0.0005 0.0002 0 0.0001 0 0 0.0001 0
Cytoplasm 0.8806 0.8649 0.8479 0.825 0.8737 0.8844 0.8059 0.8553 0.8436 0.8423 0.7155 0.8947 0.8379 0.9676 0.958 0.9821 0.9536 0.9725
Cytoplasmic Foci 0.0099 0.005 0.013 0.0037 0.014 0.0106 0.0204 0.0075 0.0195 0.0095 0.0371 0.0074 0.0463 0.0015 0.0046 0.0025 0.004 0.0018
Eisosomes 0 0 0 0 0.0001 0 0.0002 0 0.0002 0 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0053 0.0025 0.0042 0.0039 0.0094 0.0048 0.0063 0.0021 0.004 0.0036 0.006 0.0041 0.0021 0.002 0.0008 0.0012 0.0023 0.0021
Endosome 0.0041 0.002 0.0031 0.0016 0.0106 0.0051 0.0255 0.0043 0.0111 0.002 0.0421 0.005 0.0329 0.0005 0.0043 0.0004 0.0018 0.0006
Golgi 0.0003 0.0002 0.0004 0.0002 0.001 0.0002 0.0051 0.0013 0.0047 0.0002 0.0109 0.0011 0.0172 0 0.0022 0.0001 0.0001 0
Lipid Particles 0.0002 0.0001 0.0003 0.0001 0.0028 0.0001 0.0042 0.0007 0.0022 0 0.0251 0.0011 0.007 0 0.0121 0 0 0
Mitochondria 0.0004 0.0007 0.0008 0.0007 0.0009 0.0004 0.0073 0.0008 0.0043 0.0006 0.0045 0.0023 0.0092 0.0001 0.0003 0.0001 0.0004 0.0001
None 0.0274 0.0196 0.0334 0.0101 0.0268 0.0135 0.0436 0.0563 0.0379 0.0785 0.0252 0.031 0.0192 0.0108 0.0055 0.0026 0.0133 0.0017
Nuclear Periphery 0.0102 0.0068 0.007 0.0128 0.0067 0.0099 0.0089 0.0025 0.0043 0.0063 0.0072 0.0069 0.0022 0.0047 0.001 0.0008 0.002 0.0018
Nucleolus 0.0001 0.0003 0.0003 0.0004 0.0002 0.0002 0.001 0.0001 0.0003 0.0002 0.0023 0.0002 0.0002 0 0 0 0 0
Nucleus 0.0484 0.0261 0.0309 0.0562 0.0257 0.0491 0.023 0.0117 0.0168 0.0274 0.0167 0.0289 0.0035 0.0095 0.0038 0.0079 0.0033 0.017
Peroxisomes 0.0003 0.0003 0.0007 0.0002 0.0005 0.0003 0.0021 0.0006 0.0034 0.0004 0.0129 0.0007 0.01 0 0.0022 0 0.0001 0
Punctate Nuclear 0.0028 0.0041 0.0057 0.0044 0.0039 0.0044 0.0054 0.0019 0.0039 0.004 0.0209 0.0024 0.0036 0.0005 0.0017 0.0007 0.0005 0.0008
Vacuole 0.0024 0.0032 0.0032 0.0032 0.0053 0.0027 0.004 0.0015 0.0029 0.0026 0.0039 0.0021 0.0014 0.0007 0.0002 0.0001 0.0022 0.0002
Vacuole Periphery 0.0004 0.0005 0.0006 0.0008 0.0009 0.0006 0.0011 0.0003 0.0006 0.0007 0.0014 0.0008 0.0011 0.0001 0.0001 0 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.1352 6.0887 10.5423 10.9126 14.7182 2.2691 5.6433 6.2327 19.5204 17.985
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1452 2113 314 1389 1601 1642 617 1933 3053 3755 931 3322

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 780.35 911.95 1046.35 1130.21 759.05 812.65 1219.30 1110.85 769.18 868.53 1160.97 1118.94
Standard Deviation 288.63 270.93 426.96 402.49 126.09 248.41 393.78 602.89 219.25 265.92 413.44 528.51
Intensity Change Log 2 — 0.224833 0.423172 0.534398 — 0.098439 0.683786 0.549397 — 0.163894 0.557557 0.541813

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000177 0.000192 0.000333 0.004319 0.000062 0.000341 0.000256 0.001269 0.000117 0.000257 0.000282 0.002544
Bud Neck 0.004368 0.007096 0.002326 0.007508 0.014034 0.031044 0.002184 0.003933 0.009437 0.017568 0.002232 0.005427
Bud Site 0.009415 0.008886 0.004901 0.043114 0.007932 0.022666 0.005091 0.026455 0.008637 0.014912 0.005027 0.033421
Cell Periphery 0.000289 0.000127 0.000182 0.000340 0.000203 0.000151 0.000148 0.000144 0.000244 0.000138 0.000160 0.000226
Cytoplasm 0.712531* 0.754783* 0.469343* 0.607125* 0.681907* 0.607924* 0.547611* 0.704937* 0.696472* 0.690564* 0.521213* 0.664040*
Cytoplasmic Foci 0.068504 0.042326 0.019305 0.013068 0.071747 0.147217 0.008105 0.012709 0.070205 0.088193 0.011883 0.012859
Eisosomes 0.000042 0.000017 0.000050 0.000029 0.000044 0.000084 0.000026 0.000016 0.000043 0.000046 0.000034 0.000022
Endoplasmic Reticulum 0.000799 0.000506 0.002664 0.000987 0.000424 0.000507 0.001885 0.000540 0.000603 0.000506 0.002148 0.000727
Endosome 0.004120 0.003019 0.003493 0.004899 0.002699 0.015147 0.001604 0.004386 0.003375 0.008322 0.002241 0.004600
Golgi 0.000908 0.000937 0.000351 0.004997 0.000455 0.003708 0.000132 0.004084 0.000670 0.002149 0.000206 0.004466
Lipid Particles 0.002116 0.000667 0.002162 0.000587 0.001519 0.002519 0.000928 0.001132 0.001803 0.001477 0.001344 0.000904
Mitochondria 0.002047 0.000829 0.000321 0.002589 0.001226 0.005778 0.000417 0.003449 0.001617 0.002994 0.000385 0.003090
Mitotic Spindle 0.001071 0.004016 0.005691 0.056786 0.000964 0.010557 0.024400 0.030929 0.001015 0.006876 0.018090 0.041740
None 0.029807 0.017230 0.017145 0.011686 0.011852 0.006999 0.012384 0.009934 0.020391 0.012756 0.013990 0.010667
Nuclear Periphery 0.000237 0.000353 0.001243 0.000710 0.000186 0.000229 0.001244 0.000599 0.000211 0.000299 0.001244 0.000646
Nuclear Periphery Foci 0.001704 0.000684 0.003422 0.001278 0.000146 0.000544 0.002161 0.001335 0.000887 0.000623 0.002587 0.001311
Nucleolus 0.001216 0.000669 0.000525 0.000400 0.000650 0.001450 0.000337 0.000221 0.000919 0.001010 0.000400 0.000296
Nucleus 0.124061 0.113901 0.342113* 0.157953* 0.161469* 0.075056 0.304907* 0.121549 0.143678* 0.096915 0.317455* 0.136770*
Peroxisomes 0.000952 0.001713 0.005904 0.000915 0.001522 0.007341 0.000099 0.001962 0.001251 0.004174 0.002057 0.001524
Vacuole 0.034878 0.041539 0.118294 0.076015 0.040543 0.059291 0.085957 0.067737 0.037849 0.049302 0.096863 0.071198
Vacuole Periphery 0.000758 0.000508 0.000232 0.004692 0.000415 0.001447 0.000124 0.002679 0.000578 0.000919 0.000160 0.003521

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.19 -5.39 -1.93 -1.90 -1.25 -4.83 -11.84 -3.28 -1.42 -1.01 -3.37 -10.43 -2.98 -2.39 -1.58
Bud Neck -3.09 0.67 -1.37 2.21 -1.99 -6.97 8.25 8.19 13.23 -0.23 -6.51 7.85 7.09 12.74 -1.76
Bud Site 0.44 2.23 -5.13 -5.59 -6.57 -7.91 1.55 -3.82 3.74 -5.06 -6.01 2.72 -6.19 -1.28 -8.15
Cell Periphery 6.58 2.18 0.94 -5.54 -1.24 0.60 0.20 1.05 3.14 4.27 2.30 0.97 1.48 -3.00 1.47
Cytoplasm -4.57 18.72 23.96 29.96 -4.94 8.06 21.28 12.27 2.93 -12.18 1.73 28.82 24.68 22.63 -11.90
Cytoplasmic Foci 7.19 14.42 18.49 14.09 2.32 -13.51 20.49 19.74 29.56 -3.21 -5.34 25.95 27.09 30.82 -0.33
Eisosomes 10.22 -3.49 4.13 -7.99 5.81 -5.88 3.54 8.34 9.74 5.01 -1.01 0.33 9.31 6.94 7.15
Endoplasmic Reticulum 1.24 -7.80 -1.51 -5.80 8.08 -0.74 -9.00 -2.58 -2.08 8.53 0.70 -11.03 -2.29 -5.30 11.40
Endosome 2.46 0.93 4.97 3.24 3.84 -10.66 3.32 2.75 11.65 0.26 -8.57 3.75 5.51 11.80 2.61
Golgi -0.02 1.79 -0.37 -0.37 -1.57 -5.26 6.27 -1.85 3.58 -2.56 -4.23 2.72 -1.35 2.29 -2.93
Lipid Particles 2.75 -0.43 1.62 -5.93 4.05 -3.17 0.79 2.67 4.95 2.47 1.15 0.32 2.64 2.45 4.43
Mitochondria 1.45 2.03 1.23 -0.68 -3.31 -5.48 6.77 0.06 4.82 -1.51 -2.49 2.94 1.02 3.92 -2.34
Mitotic Spindle -3.22 -2.32 -9.88 -8.80 -4.74 -5.31 -5.39 -10.94 -5.11 -1.74 -6.17 -5.80 -14.65 -10.54 -4.34
None 5.57 5.34 9.70 7.33 0.26 3.67 1.45 2.69 -2.04 1.56 5.92 6.39 9.60 5.01 0.95
Nuclear Periphery -0.98 -13.12 -17.45 -6.06 2.87 -1.34 -20.10 -20.54 -17.02 9.94 -1.24 -23.58 -25.92 -9.22 9.53
Nuclear Periphery Foci 4.73 -4.65 1.13 -6.34 5.38 -3.43 -10.76 -6.66 -2.37 6.69 2.06 -9.35 -2.25 -4.97 8.41
Nucleolus 2.29 3.34 4.08 3.99 2.56 -3.97 4.93 2.98 5.34 -0.14 -0.66 4.92 4.91 6.25 0.78
Nucleus 1.54 -13.59 -12.91 -15.21 8.17 18.04 -17.10 -3.54 -21.89 15.08 12.73 -22.54 -11.42 -24.43 16.62
Peroxisomes -1.61 -0.68 3.51 3.26 0.98 -7.64 4.64 2.04 8.80 -1.65 -6.74 0.46 2.95 8.50 0.54
Vacuole -3.34 -11.90 -24.67 -23.18 -1.19 -6.39 -14.63 -23.51 -18.62 0.81 -6.54 -18.90 -33.92 -29.91 -0.51
Vacuole Periphery 1.27 2.75 -0.54 -1.02 -1.53 -6.99 6.03 -1.34 2.40 -2.45 -2.85 4.27 -1.07 0.08 -2.60
Standard name
Human Ortholog
Description Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion or in response to DNA replication stress; RGI1 has a paralog, RGI2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (72%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rgi1

Rgi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rgi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available