Standard name | |
---|---|
Human Ortholog | |
Description | Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 9 | 1 | 5 | 1 | 4 | 3 | 0 | 2 | 2 | 0 | 6 | 1 | 6 | 28 | 19 | 27 | 3 | 6 | 5 | 10 | 23 | 22 |
Bud | 0 | 3 | 0 | 4 | 1 | 2 | 13 | 8 | 7 | 3 | 8 | 3 | 3 | 3 | 5 | 1 | 1 | 1 | 0 | 2 | 1 | 1 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Site | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 5 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 8 | 2 | 3 | 1 | 3 | 0 | 2 | 0 | 0 | 2 | 3 | 3 | 5 | 9 | 8 | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 4 | 0 | 5 | 6 | 9 | 14 | 12 | 10 | 9 | 8 | 7 | 5 | 2 | 6 | 9 | 10 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 5 | 1 | 5 | 0 | 0 | 1 | 0 | 1 | 2 |
Endosome | 3 | 0 | 2 | 2 | 3 | 3 | 6 | 6 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 5 | 3 | 1 | 3 | 1 | 9 | 6 |
Golgi | 0 | 4 | 39 | 25 | 40 | 4 | 9 | 10 | 0 | 0 | 0 | 1 | 7 | 5 | 6 | 135 | 139 | 110 | 11 | 14 | 20 | 18 | 23 | 20 |
Mitochondria | 112 | 114 | 165 | 172 | 192 | 192 | 298 | 253 | 243 | 232 | 163 | 181 | 350 | 331 | 399 | 25 | 16 | 24 | 92 | 97 | 168 | 134 | 242 | 239 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 2 | 4 | 1 | 1 | 4 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 5 | 2 | 0 | 1 | 6 | 1 | 23 | 13 | 11 | 6 | 14 | 13 | 1 | 1 | 3 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 3 |
Nucleolus | 24 | 15 | 5 | 4 | 20 | 29 | 37 | 34 | 38 | 21 | 22 | 21 | 38 | 23 | 31 | 20 | 16 | 18 | 3 | 2 | 0 | 1 | 5 | 6 |
Peroxisomes | 0 | 2 | 8 | 0 | 8 | 3 | 12 | 7 | 4 | 3 | 3 | 6 | 4 | 12 | 16 | 3 | 1 | 5 | 4 | 4 | 8 | 19 | 51 | 41 |
SpindlePole | 1 | 0 | 1 | 0 | 1 | 2 | 6 | 6 | 3 | 1 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 3 | 1 |
Vac/Vac Membrane | 4 | 8 | 19 | 8 | 14 | 20 | 16 | 21 | 5 | 10 | 3 | 11 | 20 | 20 | 18 | 33 | 27 | 32 | 2 | 6 | 6 | 2 | 8 | 6 |
Unique Cell Count | 125 | 126 | 192 | 187 | 235 | 240 | 363 | 317 | 266 | 260 | 181 | 208 | 368 | 361 | 438 | 208 | 188 | 194 | 127 | 137 | 220 | 193 | 386 | 363 |
Labelled Cell Count | 154 | 152 | 261 | 225 | 304 | 276 | 447 | 374 | 328 | 292 | 224 | 250 | 435 | 407 | 501 | 271 | 234 | 242 | 127 | 137 | 220 | 193 | 386 | 363 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.9 | 18.6 | 19.6 | 21.9 | 21.4 | 17.4 | 16.4 | 14.9 | 12.9 | 11.8 | 11.2 | 12.6 | 26.6 | 25.4 | 24.3 | 34.4 | 34.5 | 33.0 | 20.0 | 20.6 | 19.5 |
Std Deviation (1e-4) | 6.1 | 5.0 | 5.3 | 6.1 | 6.3 | 6.1 | 5.3 | 4.6 | 4.1 | 3.5 | 3.6 | 4.3 | 6.8 | 6.5 | 6.6 | 12.0 | 10.0 | 11.8 | 5.0 | 5.4 | 5.2 |
Intensity Change (Log2) | – | – | – | 0.16 | 0.13 | -0.17 | -0.26 | -0.4 | -0.61 | -0.74 | -0.81 | -0.64 | 0.44 | 0.37 | 0.31 | 0.81 | 0.81 | 0.75 | 0.03 | 0.07 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -1.5 | 0 | 0 | 0 | 0 | 0 | -2.0 | 0 | -2.1 | 0 | 0 | 3.0 | 2.0 | 3.1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 0 | 0 |
Cytoplasm | 0.4 | 0.7 | 1.6 | 0.5 | 0.4 | 0.5 | 0.3 | 0.7 | 0 | 0 | 0 | 0 | 1.2 | 0 | 0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.8 | -0.9 | -6.4 | -7.1 | -6.4 | -7.7 | -7.6 | -6.4 | -6.6 | -7.5 | -7.8 | -8.5 | 9.0 | 10.5 | 7.3 |
Mitochondria | 1.9 | -1.2 | -1.6 | -1.2 | -1.7 | 1.8 | 1.1 | 1.2 | 0.3 | 3.8 | 2.1 | 1.9 | -14.8 | -15.1 | -14.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 3.6 | 0 | 0 | 0 | 3.9 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 2.6 | 3.6 | 3.2 | 3.3 | 4.2 | 2.5 | 3.6 | 3.0 | 3.3 | 1.9 | 2.2 | 2.9 | 2.5 | 2.8 |
Peroxisomes | 0 | -0.4 | 0 | -0.5 | -1.3 | -1.8 | 0 | -1.4 | -0.7 | 0 | -0.5 | -0.3 | -1.7 | 0 | -0.9 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.1 | -1.5 | -0.6 | -2.5 | -1.3 | -3.8 | -2.6 | -3.4 | -1.7 | -2.0 | -1.9 | -2.8 | 1.8 | 1.3 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 58.788 | 59.0815 | 60.0047 | 57.0128 | 61.2051 | 57.5323 | 66.8718 | 70.4886 | 67.9357 | 60.1286 | 58.4279 | 66.3399 | 60.121 | 61.4533 | 60.6676 | 47.9478 | 56.4661 | 62.5203 |
Actin | 0.0086 | 0.001 | 0.0028 | 0.0202 | 0 | 0.0001 | 0.0158 | 0.0009 | 0.0011 | 0.0161 | 0.0003 | 0 | 0.0004 | 0.0009 | 0.0011 | 0 | 0 | 0.0001 |
Bud | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0004 | 0 | 0.0001 | 0.0009 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0002 | 0.0005 | 0.0009 | 0.0004 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0.0002 | 0 | 0 | 0.0004 | 0.0005 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0004 | 0 | 0 | 0.0044 | 0 | 0 | 0.0007 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0052 | 0 | 0.0003 | 0.0033 | 0 | 0.001 | 0.0119 | 0.0001 | 0.0079 | 0.0027 | 0.001 | 0 | 0.0061 | 0.0028 | 0.0048 | 0.0001 | 0.0022 | 0 |
Eisosomes | 0.0006 | 0.0003 | 0.0008 | 0.0015 | 0.0001 | 0.0002 | 0.002 | 0.0025 | 0.0014 | 0.0004 | 0.0008 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0006 | 0 | 0 | 0.0042 | 0 | 0 | 0.001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.003 | 0.0001 | 0.0001 | 0.0062 | 0 | 0 | 0.0061 | 0.0001 | 0.0021 | 0.0109 | 0 | 0 | 0.0002 | 0.0006 | 0.0008 | 0.0001 | 0.0001 | 0 |
Golgi | 0.0054 | 0.0002 | 0.0003 | 0.0026 | 0.0001 | 0.0001 | 0.0052 | 0.0004 | 0.0006 | 0.0074 | 0 | 0.0001 | 0.0002 | 0.0014 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Lipid Particles | 0.0096 | 0.0067 | 0.0043 | 0.0047 | 0.0026 | 0.0047 | 0.0098 | 0.0034 | 0.0053 | 0.0079 | 0.0048 | 0.001 | 0.0057 | 0.0058 | 0.0033 | 0.0032 | 0.0036 | 0.0015 |
Mitochondria | 0.9391 | 0.9628 | 0.9786 | 0.9268 | 0.9929 | 0.9435 | 0.9252 | 0.9847 | 0.9545 | 0.9358 | 0.9768 | 0.9968 | 0.9469 | 0.9686 | 0.9686 | 0.9879 | 0.9758 | 0.9897 |
None | 0.0017 | 0 | 0 | 0.0094 | 0 | 0.0001 | 0.001 | 0 | 0.0008 | 0.0003 | 0.0001 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0 | 0 |
Nuclear Periphery | 0.0008 | 0.0001 | 0 | 0.004 | 0 | 0.0001 | 0.0026 | 0 | 0.0006 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0012 | 0 | 0 | 0 |
Nucleolus | 0.0068 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0107 | 0.0021 | 0.0002 | 0.0062 | 0.0001 | 0.0089 | 0 | 0.0221 | 0.0007 | 0.004 | 0.0028 | 0.0141 | 0.0006 |
Nucleus | 0.0004 | 0.0001 | 0 | 0.0011 | 0 | 0.0003 | 0.0005 | 0 | 0.0011 | 0.0001 | 0.0003 | 0 | 0.0016 | 0.0001 | 0.0004 | 0.0003 | 0.0006 | 0 |
Peroxisomes | 0.0078 | 0.001 | 0.005 | 0.0017 | 0.0002 | 0.0006 | 0.0055 | 0.0036 | 0.0025 | 0.0101 | 0.0017 | 0.0002 | 0.0021 | 0.0025 | 0.0009 | 0.0004 | 0.0006 | 0.0002 |
Punctate Nuclear | 0.0012 | 0 | 0 | 0.0062 | 0 | 0 | 0.0006 | 0 | 0.0006 | 0.0009 | 0 | 0 | 0.0006 | 0 | 0.0003 | 0 | 0.0001 | 0 |
Vacuole | 0.0008 | 0.0012 | 0.0004 | 0.0002 | 0.0003 | 0.0007 | 0.0012 | 0.0003 | 0.0018 | 0.0019 | 0.0009 | 0 | 0.0017 | 0.001 | 0.0012 | 0.0006 | 0.0006 | 0.0002 |
Vacuole Periphery | 0.0069 | 0.0249 | 0.0054 | 0.0012 | 0.0035 | 0.0378 | 0.0077 | 0.0036 | 0.0122 | 0.0038 | 0.0038 | 0.0016 | 0.0098 | 0.0141 | 0.0119 | 0.0042 | 0.0019 | 0.0071 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 47.0394 | 26.2577 | 23.1553 | 37.8052 | 42.4989 | 86.3685 | 92.7296 | 88.5556 | 85.3338 | 94.0818 |
Translational Efficiency | 0.9257 | 0.9488 | 0.9911 | 0.7279 | 0.7941 | 0.9985 | 0.8717 | 0.7417 | 0.79 | 0.791 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2023 | 1013 | 431 | 1353 | 1406 | 1684 | 1489 | 186 | 3429 | 2697 | 1920 | 1539 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1688.25 | 1948.75 | 3300.18 | 1833.53 | 2016.13 | 2053.69 | 2411.80 | 2011.12 | 1822.69 | 2014.27 | 2611.22 | 1854.99 |
Standard Deviation | 376.21 | 552.78 | 845.71 | 482.02 | 497.92 | 521.46 | 572.66 | 502.07 | 459.52 | 535.86 | 743.15 | 487.93 |
Intensity Change Log 2 | — | 0.207020 | 0.967016 | 0.119095 | — | 0.026630 | 0.258522 | -0.003589 | — | 0.111648 | 0.624759 | 0.053620 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001042 | 0.005640 | 0.013897 | 0.006231 | 0.003747 | 0.003129 | 0.003928 | 0.003606 | 0.002151 | 0.004072 | 0.006166 | 0.005914 |
Bud Neck | 0.000220 | 0.001897 | 0.006559 | 0.001814 | 0.001287 | 0.000860 | 0.001143 | 0.002003 | 0.000658 | 0.001249 | 0.002358 | 0.001836 |
Bud Site | 0.000123 | 0.001089 | 0.002535 | 0.003596 | 0.000802 | 0.000548 | 0.000744 | 0.001708 | 0.000401 | 0.000752 | 0.001146 | 0.003368 |
Cell Periphery | 0.000069 | 0.000092 | 0.000235 | 0.000598 | 0.000089 | 0.000058 | 0.000057 | 0.000216 | 0.000077 | 0.000071 | 0.000097 | 0.000551 |
Cytoplasm | 0.000093 | 0.000272 | 0.000214 | 0.000410 | 0.000239 | 0.000212 | 0.000220 | 0.000414 | 0.000153 | 0.000234 | 0.000218 | 0.000411 |
Cytoplasmic Foci | 0.012794 | 0.025111 | 0.034627 | 0.009153 | 0.026268 | 0.018158 | 0.014322 | 0.011945 | 0.018319 | 0.020769 | 0.018880 | 0.009491 |
Eisosomes | 0.001323 | 0.002283 | 0.006136 | 0.001269 | 0.002176 | 0.001037 | 0.001080 | 0.001018 | 0.001673 | 0.001505 | 0.002215 | 0.001239 |
Endoplasmic Reticulum | 0.000064 | 0.000101 | 0.000181 | 0.000273 | 0.000151 | 0.000079 | 0.000053 | 0.000191 | 0.000100 | 0.000087 | 0.000082 | 0.000263 |
Endosome | 0.000476 | 0.001398 | 0.000598 | 0.003651 | 0.000717 | 0.001135 | 0.000701 | 0.004174 | 0.000575 | 0.001234 | 0.000678 | 0.003715 |
Golgi | 0.010961 | 0.019896 | 0.009740 | 0.026128 | 0.013265 | 0.016472 | 0.013077 | 0.031158 | 0.011906 | 0.017758 | 0.012328 | 0.026736 |
Lipid Particles | 0.006711 | 0.006851 | 0.008771 | 0.004598 | 0.006739 | 0.006270 | 0.004998 | 0.002822 | 0.006722 | 0.006488 | 0.005845 | 0.004383 |
Mitochondria | 0.944456* | 0.903266* | 0.851883* | 0.907196* | 0.910464* | 0.932272* | 0.933176* | 0.901079* | 0.930518* | 0.921377* | 0.914928* | 0.906457* |
Mitotic Spindle | 0.000248 | 0.000702 | 0.008759 | 0.001205 | 0.000594 | 0.000463 | 0.003030 | 0.001967 | 0.000390 | 0.000553 | 0.004316 | 0.001297 |
None | 0.006610 | 0.003630 | 0.009097 | 0.002155 | 0.004576 | 0.002524 | 0.003277 | 0.003253 | 0.005776 | 0.002939 | 0.004583 | 0.002288 |
Nuclear Periphery | 0.000613 | 0.000272 | 0.001863 | 0.000529 | 0.000598 | 0.000251 | 0.000726 | 0.000477 | 0.000606 | 0.000259 | 0.000981 | 0.000523 |
Nuclear Periphery Foci | 0.000203 | 0.000254 | 0.000587 | 0.000221 | 0.000415 | 0.000191 | 0.000215 | 0.000209 | 0.000290 | 0.000215 | 0.000298 | 0.000219 |
Nucleolus | 0.000428 | 0.002153 | 0.003426 | 0.000445 | 0.001030 | 0.000770 | 0.000877 | 0.000470 | 0.000675 | 0.001289 | 0.001449 | 0.000448 |
Nucleus | 0.000037 | 0.000568 | 0.000233 | 0.000078 | 0.000092 | 0.000118 | 0.000059 | 0.000060 | 0.000060 | 0.000287 | 0.000098 | 0.000076 |
Peroxisomes | 0.003111 | 0.015571 | 0.015813 | 0.009166 | 0.013592 | 0.006574 | 0.007177 | 0.014625 | 0.007408 | 0.009954 | 0.009116 | 0.009826 |
Vacuole | 0.003089 | 0.003816 | 0.008770 | 0.008370 | 0.005690 | 0.004509 | 0.003520 | 0.006535 | 0.004156 | 0.004249 | 0.004698 | 0.008148 |
Vacuole Periphery | 0.007332 | 0.005136 | 0.016077 | 0.012915 | 0.007468 | 0.004373 | 0.007620 | 0.012071 | 0.007388 | 0.004659 | 0.009519 | 0.012813 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.12 | -5.19 | -4.78 | -0.44 | 2.84 | 0.66 | -0.19 | 0.14 | -0.59 | 0.39 | -3.35 | -5.13 | -3.74 | -1.75 | 0.20 |
Bud Neck | -4.85 | -6.41 | -6.46 | 0.15 | 4.63 | 1.69 | 0.52 | -1.32 | -2.22 | -1.64 | -3.35 | -6.32 | -4.91 | -2.22 | 1.49 |
Bud Site | -2.84 | -5.87 | -4.57 | -3.05 | -1.30 | 0.67 | 0.12 | -1.45 | -2.13 | -1.75 | -1.70 | -3.61 | -4.34 | -3.82 | -3.25 |
Cell Periphery | -0.56 | -2.59 | -4.00 | -3.98 | -2.68 | 1.05 | 1.20 | -2.01 | -2.62 | -2.70 | 0.19 | -0.72 | -4.16 | -4.29 | -4.05 |
Cytoplasm | -5.25 | -4.37 | -4.96 | -2.03 | -3.00 | 0.15 | 0.24 | -1.21 | -1.27 | -1.41 | -1.65 | -1.61 | -4.09 | -2.31 | -2.95 |
Cytoplasmic Foci | -4.97 | -5.42 | 2.57 | 6.24 | 6.24 | 3.30 | 4.84 | 4.88 | 2.38 | 0.94 | -1.55 | -0.37 | 6.38 | 7.06 | 5.40 |
Eisosomes | -3.95 | -6.66 | 0.18 | 3.03 | 6.42 | 6.85 | 5.79 | 5.68 | 0.11 | 0.33 | 1.25 | -2.53 | 1.74 | 1.06 | 3.24 |
Endoplasmic Reticulum | -2.59 | -3.95 | -3.86 | -3.15 | -1.53 | 2.12 | 2.97 | -0.59 | -1.93 | -2.40 | 0.79 | 1.13 | -3.29 | -3.66 | -3.77 |
Endosome | -3.42 | -0.71 | -5.17 | -3.50 | -5.02 | -1.84 | 0.08 | -2.44 | -2.14 | -2.45 | -3.76 | -0.64 | -5.60 | -4.33 | -5.34 |
Golgi | -3.83 | 0.50 | -5.35 | -1.84 | -4.72 | -1.59 | 0.10 | -2.66 | -2.18 | -2.69 | -4.01 | -0.30 | -5.70 | -3.27 | -5.29 |
Lipid Particles | -0.11 | -1.05 | 1.66 | 1.51 | 2.01 | 0.39 | 1.69 | 2.32 | 1.97 | 1.33 | 0.26 | 1.02 | 2.19 | 1.83 | 1.30 |
Mitochondria | 6.03 | 8.40 | 5.88 | -0.43 | -4.56 | -3.38 | -3.47 | 0.65 | 2.17 | 2.22 | 2.12 | 3.23 | 4.12 | 2.41 | 1.30 |
Mitotic Spindle | -1.73 | -2.81 | -2.83 | -1.28 | 2.47 | 0.72 | -2.96 | -2.30 | -2.55 | 1.07 | -1.17 | -4.22 | -2.97 | -2.38 | 3.08 |
None | 8.05 | -2.86 | 14.18 | 4.87 | 8.24 | 7.47 | 3.22 | 1.81 | -1.05 | 0.03 | 12.64 | 3.26 | 13.86 | 3.18 | 6.48 |
Nuclear Periphery | 1.08 | -3.03 | 0.25 | -2.19 | 4.57 | 1.92 | -0.64 | 0.49 | -1.30 | 1.28 | 1.74 | -1.71 | 0.38 | -2.55 | 3.32 |
Nuclear Periphery Foci | -0.67 | -2.26 | -0.26 | 0.52 | 2.22 | 2.08 | 1.85 | 1.81 | -0.28 | 0.10 | 1.21 | -0.11 | 1.08 | -0.09 | 1.36 |
Nucleolus | -1.70 | -4.65 | -0.18 | 1.68 | 4.62 | 1.17 | 0.58 | 2.64 | 1.59 | 1.81 | -1.55 | -3.52 | 2.05 | 2.12 | 4.62 |
Nucleus | -1.12 | -3.79 | -2.07 | 1.03 | 2.77 | -0.61 | 1.31 | 1.22 | 1.30 | 0.01 | -1.28 | -2.28 | -0.89 | 1.17 | 0.94 |
Peroxisomes | -6.08 | -4.27 | -3.77 | 2.55 | 2.01 | 3.66 | 3.20 | -0.16 | -1.31 | -1.20 | -2.11 | -1.33 | -1.42 | 0.07 | -0.37 |
Vacuole | -0.97 | -2.64 | -4.08 | -3.47 | 0.13 | 1.00 | 2.03 | -0.29 | -0.65 | -0.98 | -0.21 | -0.69 | -3.33 | -3.22 | -2.70 |
Vacuole Periphery | 1.96 | -4.15 | -3.49 | -4.72 | 1.30 | 3.20 | -0.13 | -1.09 | -1.86 | -1.06 | 3.93 | -2.22 | -3.72 | -5.82 | -2.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|