Standard name | |
---|---|
Human Ortholog | |
Description | Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; also has mRNA binding activity; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 4 | 0 | 3 | 1 | 1 | 4 | 4 | – | 6 | 9 | 7 | 6 | 7 | 3 | 1 | 3 | 0 | 1 | 3 | 0 | 1 | 1 | 2 |
Bud | 0 | 2 | 0 | 1 | 1 | 8 | 9 | 9 | – | 9 | 15 | 17 | 5 | 3 | 1 | 0 | 0 | 0 | 4 | 5 | 0 | 5 | 5 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 0 | 11 | 6 | 19 | 23 | 23 | – | 35 | 48 | 28 | 13 | 34 | 22 | 0 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 241 | 206 | 41 | 101 | 119 | 181 | 252 | 252 | – | 278 | 193 | 197 | 319 | 427 | 524 | 76 | 72 | 65 | 14 | 13 | 0 | 7 | 6 | 10 |
Endoplasmic Reticulum | 73 | 78 | 24 | 19 | 29 | 30 | 37 | 30 | – | 28 | 6 | 12 | 176 | 157 | 88 | 4 | 4 | 4 | 226 | 216 | 44 | 159 | 244 | 305 |
Endosome | 15 | 13 | 0 | 3 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 2 | 5 | 7 | 6 | 9 | 6 | 3 | 1 | 0 | 4 | 2 |
Golgi | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 6 | 1 | 0 | 1 | 3 | 2 |
Mitochondria | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 1 | – | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 4 |
Nucleus | 6 | 0 | 2 | 0 | 0 | 0 | 2 | 2 | – | 0 | 0 | 1 | 1 | 1 | 0 | 15 | 13 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 242 | 82 | 19 | 6 | 7 | 3 | 9 | 11 | – | 7 | 2 | 7 | 34 | 26 | 41 | 119 | 131 | 121 | 347 | 175 | 70 | 152 | 144 | 152 |
Unique Cell Count | 527 | 356 | 81 | 125 | 154 | 218 | 299 | 295 | 314 | 219 | 223 | 490 | 585 | 631 | 199 | 206 | 177 | 619 | 426 | 120 | 332 | 418 | 496 | |
Labelled Cell Count | 584 | 388 | 89 | 145 | 164 | 244 | 338 | 332 | 365 | 274 | 271 | 554 | 658 | 685 | 226 | 237 | 209 | 619 | 426 | 120 | 332 | 418 | 496 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.1 | 14.0 | 8.6 | 11.6 | 12.2 | 10.7 | 10.0 | 10.0 | – | 10.2 | 10.8 | 10.6 | 13.4 | 12.7 | 10.9 | 12.8 | 13.9 | 14.8 | 15.6 | 16.7 | 16.9 |
Std Deviation (1e-4) | 5.7 | 8.5 | 2.0 | 4.5 | 4.5 | 2.0 | 2.1 | 2.1 | – | 2.6 | 2.5 | 2.5 | 4.6 | 5.0 | 4.3 | 7.1 | 8.7 | 10.6 | 6.4 | 7.3 | 6.6 |
Intensity Change (Log2) | – | – | – | 0.44 | 0.51 | 0.32 | 0.22 | 0.22 | – | 0.25 | 0.33 | 0.31 | 0.65 | 0.56 | 0.35 | 0.57 | 0.7 | 0.78 | 0.86 | 0.96 | 0.98 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 2.7 | 0 | 0 | – | 3.1 | 4.6 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.6 | 4.2 | 5.7 | 6.4 | 6.7 | – | 7.7 | 7.0 | 7.1 | 2.5 | 4.1 | 6.8 | -1.9 | -2.4 | -2.1 |
Endoplasmic Reticulum | -2.5 | -1.9 | -3.2 | -3.8 | -4.4 | – | -4.9 | -6.9 | -5.8 | 1.1 | -0.5 | -3.6 | -7.0 | -7.1 | -6.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.0 | -4.4 | -6.5 | -6.2 | -5.8 | – | -6.9 | -6.8 | -5.6 | -4.7 | -6.4 | -5.2 | 5.5 | 6.1 | 6.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.0731 | 9.503 | 9.4207 | 9.143 | 8.7795 | 9.3297 | 9.0594 | 8.9961 | 12.5729 | 8.5231 | 12.3186 | 9.0239 | 9.9645 | 12.4674 | 10.7611 | 10.918 | 10.2616 | 10.3451 |
Actin | 0.0002 | 0.0005 | 0.0008 | 0.0003 | 0.0001 | 0.0001 | 0.0015 | 0.0012 | 0.0055 | 0.0003 | 0.0009 | 0.0012 | 0.0096 | 0.001 | 0.0051 | 0.0081 | 0.0001 | 0.0073 |
Bud | 0.0008 | 0.0004 | 0.0027 | 0.0099 | 0.0004 | 0.0008 | 0.001 | 0.0162 | 0.0044 | 0.017 | 0.0036 | 0.0058 | 0.0004 | 0.0003 | 0.0007 | 0.0003 | 0.0003 | 0.0007 |
Bud Neck | 0.0006 | 0.0017 | 0.0015 | 0.0011 | 0.0049 | 0.0027 | 0.0005 | 0.0018 | 0.0023 | 0.0019 | 0.002 | 0.0021 | 0.0015 | 0.0018 | 0.0011 | 0.0154 | 0.0034 | 0.0051 |
Bud Periphery | 0.0009 | 0.0009 | 0.0021 | 0.0047 | 0.0015 | 0.0012 | 0.0035 | 0.0177 | 0.0122 | 0.0353 | 0.0243 | 0.0262 | 0.0008 | 0.0009 | 0.0016 | 0.0013 | 0.0019 | 0.0029 |
Bud Site | 0.0033 | 0.0052 | 0.0118 | 0.0023 | 0.001 | 0.0005 | 0.0017 | 0.0184 | 0.0028 | 0.002 | 0.0008 | 0.0012 | 0.0018 | 0.0038 | 0.003 | 0.0016 | 0.0002 | 0.0005 |
Cell Periphery | 0.0181 | 0.0152 | 0.0133 | 0.0297 | 0.0226 | 0.0116 | 0.0208 | 0.05 | 0.0374 | 0.0324 | 0.0209 | 0.0151 | 0.021 | 0.0418 | 0.0544 | 0.0289 | 0.0338 | 0.0258 |
Cytoplasm | 0.1481 | 0.2049 | 0.1327 | 0.1797 | 0.1583 | 0.1207 | 0.0837 | 0.096 | 0.0855 | 0.0418 | 0.0782 | 0.0754 | 0.1473 | 0.1452 | 0.0663 | 0.0233 | 0.0276 | 0.0873 |
Cytoplasmic Foci | 0.0055 | 0.0084 | 0.0098 | 0.005 | 0.0051 | 0.0075 | 0.0057 | 0.0026 | 0.0121 | 0.004 | 0.0017 | 0.0035 | 0.0152 | 0.0041 | 0.0063 | 0.0075 | 0.0006 | 0.0056 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.5326 | 0.3732 | 0.4662 | 0.4257 | 0.285 | 0.4599 | 0.3308 | 0.2886 | 0.422 | 0.3467 | 0.3586 | 0.3775 | 0.4348 | 0.4502 | 0.482 | 0.4703 | 0.5191 | 0.4236 |
Endosome | 0.0392 | 0.0536 | 0.054 | 0.0485 | 0.0434 | 0.0489 | 0.0313 | 0.0074 | 0.0316 | 0.0522 | 0.029 | 0.0222 | 0.0569 | 0.0183 | 0.0257 | 0.04 | 0.0122 | 0.022 |
Golgi | 0.0014 | 0.002 | 0.0027 | 0.0017 | 0.0014 | 0.0013 | 0.0016 | 0.0007 | 0.005 | 0.0038 | 0.0016 | 0.0009 | 0.0084 | 0.0016 | 0.0016 | 0.0102 | 0.0003 | 0.0028 |
Lipid Particles | 0.0179 | 0.0134 | 0.0133 | 0.0126 | 0.0209 | 0.0146 | 0.0271 | 0.0164 | 0.0257 | 0.0739 | 0.0049 | 0.0112 | 0.0496 | 0.0222 | 0.0225 | 0.0581 | 0.0133 | 0.017 |
Mitochondria | 0.0019 | 0.0006 | 0.0015 | 0.0024 | 0.0005 | 0.0008 | 0.0006 | 0.0006 | 0.0014 | 0.0009 | 0.004 | 0.0012 | 0.0064 | 0.0016 | 0.0011 | 0.0013 | 0.0001 | 0.0013 |
None | 0.0019 | 0.0016 | 0.002 | 0.0008 | 0.0004 | 0.0006 | 0.3397 | 0.276 | 0.1212 | 0.1411 | 0.0976 | 0.2215 | 0.0083 | 0.0005 | 0.0008 | 0.0005 | 0.0001 | 0.0004 |
Nuclear Periphery | 0.0183 | 0.0201 | 0.0169 | 0.0185 | 0.0198 | 0.0203 | 0.0201 | 0.0118 | 0.0231 | 0.0123 | 0.039 | 0.0241 | 0.0508 | 0.0169 | 0.0161 | 0.0212 | 0.0242 | 0.0345 |
Nucleolus | 0.0014 | 0.0007 | 0.0023 | 0.0006 | 0.0033 | 0.0003 | 0.0005 | 0.0011 | 0.0012 | 0.0005 | 0.0005 | 0.0014 | 0.0007 | 0.0004 | 0.0006 | 0.0008 | 0.0001 | 0.0003 |
Nucleus | 0.0058 | 0.0106 | 0.0085 | 0.0047 | 0.0141 | 0.0065 | 0.0047 | 0.0113 | 0.0098 | 0.0023 | 0.0081 | 0.0206 | 0.0162 | 0.003 | 0.0146 | 0.0022 | 0.0025 | 0.0079 |
Peroxisomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0073 | 0.0001 | 0.0001 | 0.0002 | 0.0086 | 0.001 | 0.0051 | 0.0014 | 0 | 0.0131 |
Punctate Nuclear | 0.0008 | 0.0009 | 0.0019 | 0.0002 | 0.0005 | 0.0029 | 0.0042 | 0.0008 | 0.0063 | 0.0004 | 0.0003 | 0.0023 | 0.0063 | 0.0003 | 0.0006 | 0.002 | 0.0001 | 0.0013 |
Vacuole | 0.1972 | 0.2803 | 0.2495 | 0.2432 | 0.4075 | 0.2922 | 0.1176 | 0.1787 | 0.1777 | 0.2271 | 0.3033 | 0.1788 | 0.1506 | 0.2817 | 0.2869 | 0.2996 | 0.3512 | 0.3342 |
Vacuole Periphery | 0.0038 | 0.0054 | 0.0063 | 0.0081 | 0.0093 | 0.0066 | 0.0029 | 0.0021 | 0.0055 | 0.0039 | 0.0204 | 0.0074 | 0.0046 | 0.0034 | 0.0039 | 0.0059 | 0.0087 | 0.0064 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 335.2652 | 343.9671 | 527.3676 | 647.2756 | 557.5719 | 330.8381 | 523.5951 | 635.1552 | 710.391 | 718.089 |
Translational Efficiency | 2.6034 | 3.0475 | 2.2775 | 1.8493 | 2.1296 | 3.2678 | 2.5632 | 1.8473 | 2.1156 | 2.0709 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1397 | 1666 | 741 | 158 | 1650 | 1690 | 1586 | 1564 | 3047 | 3356 | 2327 | 1722 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1530.74 | 1338.14 | 1314.12 | 1234.01 | 1962.37 | 1177.23 | 1468.21 | 1170.72 | 1764.47 | 1257.11 | 1419.14 | 1176.53 |
Standard Deviation | 683.44 | 476.90 | 282.22 | 331.41 | 742.62 | 300.48 | 329.93 | 200.04 | 747.70 | 406.01 | 323.59 | 216.23 |
Intensity Change Log 2 | — | -0.194000 | -0.220132 | -0.310875 | — | -0.737201 | -0.418539 | -0.745201 | — | -0.473741 | -0.328219 | -0.538637 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000836 | 0.000202 | 0.001521 | 0.000972 | 0.000639 | 0.000196 | 0.001266 | 0.001818 | 0.000729 | 0.000199 | 0.001347 | 0.001740 |
Bud Neck | 0.003477 | 0.003674 | 0.002089 | 0.005500 | 0.002145 | 0.001725 | 0.001797 | 0.003044 | 0.002756 | 0.002693 | 0.001890 | 0.003269 |
Bud Site | 0.022768 | 0.009424 | 0.011744 | 0.009809 | 0.010313 | 0.004243 | 0.011749 | 0.014628 | 0.016023 | 0.006815 | 0.011747 | 0.014185 |
Cell Periphery | 0.124960* | 0.049439 | 0.016078 | 0.020643 | 0.142509* | 0.039573 | 0.033476 | 0.024573 | 0.134463* | 0.044471 | 0.027936 | 0.024213 |
Cytoplasm | 0.029261 | 0.009425 | 0.090448 | 0.033724 | 0.010563 | 0.007798 | 0.037201 | 0.041485 | 0.019136 | 0.008605 | 0.054157 | 0.040773 |
Cytoplasmic Foci | 0.005714 | 0.003616 | 0.011346 | 0.006834 | 0.002443 | 0.004640 | 0.004233 | 0.013800 | 0.003943 | 0.004131 | 0.006498 | 0.013160 |
Eisosomes | 0.000181 | 0.000191 | 0.000089 | 0.000147 | 0.000356 | 0.000049 | 0.000250 | 0.000335 | 0.000276 | 0.000119 | 0.000199 | 0.000318 |
Endoplasmic Reticulum | 0.547197* | 0.466108* | 0.678099* | 0.620036* | 0.602849* | 0.503086* | 0.814587* | 0.826307* | 0.577333* | 0.484729* | 0.771124* | 0.807381* |
Endosome | 0.017019 | 0.016009 | 0.024492 | 0.022682 | 0.011598 | 0.014529 | 0.010395 | 0.014515 | 0.014083 | 0.015264 | 0.014884 | 0.015264 |
Golgi | 0.004673 | 0.001647 | 0.009609 | 0.001589 | 0.007119 | 0.002196 | 0.004253 | 0.005468 | 0.005998 | 0.001923 | 0.005959 | 0.005112 |
Lipid Particles | 0.009901 | 0.003415 | 0.027566 | 0.009005 | 0.016358 | 0.002568 | 0.021384 | 0.019383 | 0.013397 | 0.002988 | 0.023353 | 0.018431 |
Mitochondria | 0.000746 | 0.000842 | 0.000622 | 0.000073 | 0.000792 | 0.000986 | 0.000682 | 0.000788 | 0.000771 | 0.000915 | 0.000663 | 0.000723 |
Mitotic Spindle | 0.000090 | 0.000071 | 0.000335 | 0.000040 | 0.000149 | 0.000208 | 0.000293 | 0.000232 | 0.000122 | 0.000140 | 0.000307 | 0.000215 |
None | 0.001702 | 0.000176 | 0.000660 | 0.000026 | 0.000575 | 0.000503 | 0.000620 | 0.001736 | 0.001091 | 0.000341 | 0.000633 | 0.001580 |
Nuclear Periphery | 0.000761 | 0.000685 | 0.000710 | 0.002349 | 0.001587 | 0.000366 | 0.000418 | 0.000245 | 0.001208 | 0.000524 | 0.000511 | 0.000438 |
Nuclear Periphery Foci | 0.003281 | 0.001119 | 0.027146 | 0.018994 | 0.003605 | 0.001114 | 0.004026 | 0.002920 | 0.003456 | 0.001117 | 0.011388 | 0.004395 |
Nucleolus | 0.000200 | 0.000272 | 0.000059 | 0.000132 | 0.000111 | 0.000189 | 0.000190 | 0.000041 | 0.000152 | 0.000230 | 0.000148 | 0.000049 |
Nucleus | 0.001758 | 0.000681 | 0.001491 | 0.005927 | 0.000572 | 0.000385 | 0.000841 | 0.000536 | 0.001116 | 0.000532 | 0.001048 | 0.001030 |
Peroxisomes | 0.000658 | 0.000752 | 0.000276 | 0.000284 | 0.000752 | 0.000709 | 0.001031 | 0.002616 | 0.000709 | 0.000730 | 0.000790 | 0.002402 |
Vacuole | 0.216131* | 0.421540* | 0.091421 | 0.239268* | 0.177319 | 0.405592* | 0.049193 | 0.023478 | 0.195114 | 0.413509* | 0.062640 | 0.043278 |
Vacuole Periphery | 0.008686 | 0.010714 | 0.004198 | 0.001966 | 0.007647 | 0.009345 | 0.002116 | 0.002052 | 0.008124 | 0.010024 | 0.002779 | 0.002044 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 4.51 | -1.21 | -0.67 | -1.99 | 0.45 | 4.91 | -2.99 | -2.87 | -4.05 | -1.19 | 6.55 | -2.64 | -2.70 | -4.27 | -0.89 |
Bud Neck | -0.32 | 2.94 | -1.39 | -1.20 | -2.28 | 1.31 | 0.92 | -1.73 | -2.65 | -2.36 | 0.17 | 2.86 | -1.08 | -1.11 | -2.85 |
Bud Site | 5.21 | 3.93 | 3.04 | -0.29 | 0.40 | 4.57 | -0.74 | -2.06 | -5.80 | -1.25 | 6.62 | 2.57 | 1.00 | -4.42 | -1.23 |
Cell Periphery | 14.20 | 21.63 | 14.03 | 4.61 | -0.75 | 21.08* | 22.67 | 24.76* | 5.34 | 3.31 | 24.94 | 30.21 | 30.16 | 8.48 | 1.61 |
Cytoplasm | 7.54 | -9.33 | -0.77 | -3.81 | 6.23 | 2.09 | -9.68 | -11.11 | -12.56 | -1.01 | 7.42 | -11.94 | -7.92 | -13.08 | 3.89 |
Cytoplasmic Foci | 2.07 | -3.58 | -0.63 | -1.92 | 2.19 | -2.64 | -2.38 | -8.17 | -6.16 | -6.59 | -0.30 | -3.43 | -7.17 | -7.03 | -4.93 |
Eisosomes | -0.06 | 2.90 | 0.88 | 0.32 | -2.03 | 6.03 | 1.80 | 0.28 | -5.37 | -1.40 | 2.14 | 2.08 | -0.73 | -2.42 | -2.26 |
Endoplasmic Reticulum | 6.70 | -8.14 | -2.27 | -5.04 | 1.88 | 8.79 | -19.70 | -20.90 | -31.72 | -1.42 | 11.18 | -21.78 | -24.84 | -36.99 | -4.17 |
Endosome | 0.75 | -4.09 | -1.35 | -1.62 | 0.46 | -2.50 | 1.36 | -2.37 | -0.00 | -3.23 | -1.28 | -1.06 | -0.97 | -0.02 | -0.19 |
Golgi | 4.60 | -4.73 | 4.81 | 0.12 | 8.39 | 5.57 | 3.45 | 1.92 | -4.73 | -1.93 | 7.26 | -0.00 | 1.40 | -6.00 | 1.52 |
Lipid Particles | 6.07 | -6.27 | 0.41 | -3.19 | 5.87 | 9.19 | -2.27 | -1.34 | -9.30 | 0.85 | 10.96 | -5.88 | -2.76 | -9.50 | 2.34 |
Mitochondria | -0.62 | 1.08 | 8.38 | 6.07 | 4.93 | -0.66 | 1.05 | 0.13 | 0.71 | -0.86 | -0.86 | 1.41 | 0.63 | 1.17 | -0.57 |
Mitotic Spindle | 0.36 | -3.57 | 3.92 | 0.75 | 4.25 | -0.56 | -0.78 | -0.57 | -0.13 | 0.27 | -0.35 | -1.43 | -0.71 | -0.51 | 0.52 |
None | 3.81 | 2.41 | 4.15 | 1.82 | 3.31 | 0.23 | -0.24 | -1.43 | -1.42 | -1.39 | 2.88 | 2.19 | -0.63 | -1.67 | -1.30 |
Nuclear Periphery | 0.22 | 0.39 | -2.65 | -2.41 | -2.69 | 13.02* | 13.31 | 15.23 | 2.12 | 3.84 | 3.80 | 11.88 | 9.53 | 0.45 | 1.09 |
Nuclear Periphery Foci | 4.52 | -8.50 | -2.70 | -3.07 | 1.26 | 4.38 | -0.59 | 1.09 | -4.30 | 2.08 | 6.17 | -7.83 | -1.26 | -4.95 | 6.11 |
Nucleolus | -1.99 | 6.21 | 1.21 | 2.36 | -1.43 | -1.41 | -0.52 | 6.77 | 2.70 | 0.96 | -2.37 | 0.01 | 8.05 | 5.68 | 0.93 |
Nucleus | 1.71 | 0.51 | -1.64 | -2.12 | -1.82 | 2.48 | -1.65 | 0.76 | -2.11 | 2.11 | 2.00 | 0.35 | 0.24 | -2.08 | -0.07 |
Peroxisomes | -0.46 | 4.14 | 3.77 | 1.99 | -0.12 | 0.20 | -1.19 | -5.24 | -5.09 | -3.98 | -0.23 | -0.50 | -5.35 | -4.87 | -4.73 |
Vacuole | -21.34 | 13.65 | -1.07 | 7.85 | -6.47 | -25.55 | 20.68 | 27.31 | 52.30* | 7.60 | -33.38 | 25.82 | 29.55 | 63.72* | 4.93 |
Vacuole Periphery | -1.91 | 4.85 | 7.68 | 11.74 | 4.35 | -1.92 | 8.87 | 8.70 | 10.83 | 0.24 | -2.77 | 10.08 | 11.07 | 15.34 | 2.38 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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