Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 21 | 10 | 13 | 7 | 12 | 10 | 11 | 8 | 14 | 12 | 11 | 11 | 14 | 5 | 24 | 15 | 23 | 4 | 8 | 4 | 9 | 7 | 4 |
Bud | 1 | 1 | 0 | 2 | 9 | 0 | 4 | 3 | 6 | 10 | 9 | 4 | 0 | 0 | 1 | 3 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 2 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 13 | 21 | 32 | 16 | 1 | 20 | 17 | 12 | 22 | 19 | 23 | 25 | 56 | 98 | 110 | 42 | 50 | 28 | 1 | 4 | 8 | 10 | 7 | 15 |
Cytoplasm | 41 | 62 | 28 | 27 | 27 | 76 | 82 | 91 | 90 | 69 | 69 | 97 | 43 | 82 | 116 | 93 | 55 | 73 | 6 | 17 | 3 | 22 | 17 | 15 |
Endoplasmic Reticulum | 291 | 230 | 181 | 102 | 43 | 134 | 81 | 104 | 69 | 80 | 60 | 76 | 255 | 322 | 364 | 4 | 8 | 4 | 316 | 274 | 215 | 335 | 315 | 334 |
Endosome | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 3 | 1 | 7 | 1 | 2 | 1 | 4 | 1 | 3 |
Golgi | 2 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 15 | 26 | 8 | 2 | 2 | 1 | 4 | 3 | 3 |
Mitochondria | 4 | 7 | 2 | 8 | 23 | 25 | 40 | 23 | 110 | 69 | 99 | 112 | 0 | 1 | 6 | 23 | 9 | 8 | 3 | 6 | 1 | 25 | 9 | 11 |
Nucleus | 0 | 2 | 0 | 0 | 3 | 3 | 4 | 3 | 1 | 5 | 1 | 3 | 1 | 1 | 0 | 5 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 2 | 5 | 0 | 6 | 4 | 1 | 3 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 18 | 13 | 11 | 10 | 4 | 46 | 85 | 83 | 50 | 58 | 39 | 26 | 9 | 6 | 45 | 55 | 84 | 76 | 4 | 6 | 0 | 14 | 4 | 11 |
Unique Cell Count | 334 | 306 | 225 | 139 | 93 | 263 | 273 | 295 | 286 | 263 | 249 | 285 | 322 | 437 | 528 | 224 | 214 | 209 | 350 | 327 | 240 | 437 | 379 | 410 |
Labelled Cell Count | 381 | 359 | 264 | 180 | 117 | 322 | 329 | 331 | 363 | 329 | 315 | 359 | 378 | 525 | 652 | 271 | 250 | 238 | 350 | 327 | 240 | 437 | 379 | 410 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 9.3 | 9.9 | 9.9 | 6.6 | 8.4 | 7.5 | 7.3 | 6.3 | 6.7 | 6.3 | 5.8 | 10.6 | 10.1 | 9.4 | 8.7 | 11.5 | 10.4 | 10.2 | 10.9 | 10.4 |
Std Deviation (1e-4) | 1.5 | 1.5 | 1.3 | 1.7 | 1.3 | 1.4 | 1.3 | 1.1 | 1.1 | 1.3 | 1.0 | 1.2 | 1.5 | 1.3 | 1.3 | 1.9 | 2.6 | 2.2 | 1.7 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.58 | -0.24 | -0.39 | -0.43 | -0.64 | -0.55 | -0.64 | -0.75 | 0.1 | 0.04 | -0.07 | -0.18 | 0.23 | 0.07 | 0.05 | 0.14 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.9 | 0 | 0.1 | -0.4 | -0.4 | -1.0 | 0.4 | 0.2 | -0.3 | -0.6 | -0.8 | 0 | 2.5 | 1.2 | 2.6 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | -3.5 | -2.4 | -3.0 | -4.1 | -2.4 | -2.5 | -1.7 | -1.9 | 1.0 | 2.5 | 2.1 | 1.3 | 2.5 | -0.2 |
Cytoplasm | 1.8 | 3.6 | 4.4 | 4.7 | 4.9 | 5.1 | 3.8 | 4.1 | 5.6 | 0.3 | 2.1 | 3.0 | 6.9 | 3.5 | 5.5 |
Endoplasmic Reticulum | -1.6 | -6.1 | -6.8 | -11.3 | -10.3 | -12.6 | -11.0 | -12.3 | -12.1 | -0.4 | -1.9 | -3.2 | -16.9 | -16.2 | -16.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 5.4 | 0 |
Mitochondria | 0 | 7.2 | 4.2 | 5.5 | 3.6 | 10.2 | 7.9 | 10.3 | 10.3 | 0 | 0 | 0 | 4.3 | 2.2 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.9 | 0 | 4.3 | 7.4 | 6.8 | 4.4 | 5.4 | 3.8 | 1.8 | -1.3 | -2.7 | 1.7 | 5.9 | 8.7 | 8.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.7 | 11.2311 | 10.9952 | 10.4036 | 9.8934 | 11.2026 | 12.5394 | 12.8722 | 12.6039 | 11.6301 | 11.3983 | 12.6698 | 9.7946 | 9.423 | 9.3851 | 9.8857 | 9.4595 | 9.2115 |
Actin | 0.0223 | 0.0015 | 0.0118 | 0.0132 | 0.0596 | 0.0013 | 0.0004 | 0.0002 | 0.0006 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0015 | 0.0014 | 0.0013 | 0 | 0.0002 |
Bud | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0004 | 0.0003 | 0.0044 | 0.0005 | 0.0129 | 0.0014 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0.0003 |
Bud Periphery | 0.0004 | 0.0001 | 0.0008 | 0.0003 | 0.0007 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 |
Bud Site | 0.0006 | 0.0006 | 0.0104 | 0.0001 | 0.0031 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0004 | 0.0006 | 0.0001 | 0 | 0.0001 |
Cell Periphery | 0.0058 | 0.0115 | 0.0101 | 0.0045 | 0.0031 | 0.0033 | 0.0048 | 0.0109 | 0.0031 | 0.0034 | 0.0073 | 0.0017 | 0.0038 | 0.0066 | 0.0038 | 0.0033 | 0.0007 | 0.0018 |
Cytoplasm | 0.0284 | 0.0565 | 0.0522 | 0.0084 | 0.0312 | 0.0428 | 0.0198 | 0.0267 | 0.0208 | 0.0171 | 0.0203 | 0.0231 | 0.0273 | 0.0716 | 0.0457 | 0.0635 | 0.0046 | 0.026 |
Cytoplasmic Foci | 0.0097 | 0.0031 | 0.0046 | 0.0014 | 0.0057 | 0.0018 | 0.0008 | 0.0026 | 0.0009 | 0.001 | 0.0003 | 0.001 | 0.0005 | 0.0019 | 0.0018 | 0.0019 | 0.0001 | 0.0008 |
Eisosomes | 0.0003 | 0.0001 | 0.0005 | 0.008 | 0.0008 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.8426 | 0.8794 | 0.8613 | 0.9152 | 0.7923 | 0.9269 | 0.9525 | 0.9323 | 0.9616 | 0.9588 | 0.9631 | 0.9533 | 0.9549 | 0.888 | 0.9211 | 0.9116 | 0.9899 | 0.9581 |
Endosome | 0.0189 | 0.0056 | 0.0057 | 0.0068 | 0.0224 | 0.0029 | 0.0039 | 0.0072 | 0.0025 | 0.0046 | 0.0013 | 0.0046 | 0.0017 | 0.0073 | 0.0045 | 0.0028 | 0.0009 | 0.0029 |
Golgi | 0.0048 | 0.0068 | 0.0095 | 0.0108 | 0.0271 | 0.001 | 0.0015 | 0.0008 | 0.0012 | 0.001 | 0.0005 | 0.0013 | 0.0005 | 0.0025 | 0.003 | 0.0031 | 0.0002 | 0.0006 |
Lipid Particles | 0.0181 | 0.0238 | 0.0122 | 0.0089 | 0.0098 | 0.0053 | 0.0057 | 0.0074 | 0.0042 | 0.0061 | 0.0028 | 0.0058 | 0.0028 | 0.008 | 0.0067 | 0.0052 | 0.0008 | 0.0027 |
Mitochondria | 0.0018 | 0.0002 | 0.0007 | 0.0074 | 0.0065 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0007 | 0.0002 | 0 | 0.0001 |
None | 0.0069 | 0.0002 | 0.0014 | 0.0019 | 0.004 | 0.0006 | 0.0026 | 0.0009 | 0.0006 | 0.0006 | 0.0002 | 0.0032 | 0.0006 | 0.0008 | 0.0015 | 0.0011 | 0.0001 | 0.0009 |
Nuclear Periphery | 0.0237 | 0.0057 | 0.0066 | 0.0068 | 0.0095 | 0.0035 | 0.0044 | 0.0039 | 0.0023 | 0.0028 | 0.0019 | 0.0027 | 0.0049 | 0.0063 | 0.0037 | 0.0029 | 0.0016 | 0.003 |
Nucleolus | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.002 | 0.0005 | 0.0012 | 0.0004 | 0.0012 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0004 | 0.0006 | 0.0006 | 0.0003 | 0.0001 | 0.0005 |
Peroxisomes | 0.0066 | 0.0001 | 0.0003 | 0.0007 | 0.0019 | 0.0046 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0013 | 0.0002 | 0.0022 | 0.0002 | 0.0032 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0013 | 0.0001 | 0 | 0.0002 |
Vacuole | 0.0038 | 0.0035 | 0.0033 | 0.0034 | 0.0033 | 0.0027 | 0.0017 | 0.0047 | 0.0012 | 0.0028 | 0.0009 | 0.0016 | 0.0019 | 0.0033 | 0.0024 | 0.0013 | 0.0008 | 0.0017 |
Vacuole Periphery | 0.0015 | 0.0002 | 0.0003 | 0.0009 | 0.0009 | 0.0002 | 0.0002 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 59.7999 | 48.2841 | 43.5065 | 59.1552 | 70.03 | 51.4123 | 57.8285 | 67.0773 | 57.5373 | 60.5769 |
Translational Efficiency | 1.7266 | 1.9111 | 1.9811 | 1.2659 | 1.4235 | 2.3995 | 1.4249 | 1.5436 | 1.7497 | 1.491 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
943 | 1694 | 2262 | 1262 | 1593 | 1832 | 1530 | 1493 | 2536 | 3526 | 3792 | 2755 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1034.21 | 1198.62 | 1327.10 | 1402.53 | 957.27 | 1169.94 | 1272.23 | 1391.85 | 985.88 | 1183.72 | 1304.96 | 1396.74 |
Standard Deviation | 144.13 | 165.66 | 169.60 | 215.58 | 132.08 | 161.86 | 190.30 | 235.21 | 141.65 | 164.32 | 180.26 | 226.49 |
Intensity Change Log 2 | — | 0.212845 | 0.359748 | 0.439502 | — | 0.289437 | 0.410362 | 0.540006 | — | 0.250169 | 0.384299 | 0.488687 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001049 | 0.001841 | 0.001798 | 0.004077 | 0.000842 | 0.002971 | 0.004663 | 0.003942 | 0.000919 | 0.002428 | 0.002954 | 0.004004 |
Bud Neck | 0.000400 | 0.000501 | 0.000578 | 0.002167 | 0.000614 | 0.001356 | 0.002168 | 0.004535 | 0.000535 | 0.000945 | 0.001219 | 0.003450 |
Bud Site | 0.003429 | 0.004722 | 0.005070 | 0.019443 | 0.004352 | 0.007342 | 0.018209 | 0.029035 | 0.004009 | 0.006083 | 0.010371 | 0.024641 |
Cell Periphery | 0.007823 | 0.006037 | 0.008349 | 0.032862 | 0.008497 | 0.007934 | 0.004992 | 0.018075 | 0.008246 | 0.007023 | 0.006995 | 0.024849 |
Cytoplasm | 0.041181 | 0.017244 | 0.026606 | 0.027742 | 0.043752 | 0.057054 | 0.041325 | 0.060308 | 0.042796 | 0.037928 | 0.032545 | 0.045390 |
Cytoplasmic Foci | 0.006620 | 0.008144 | 0.001882 | 0.005099 | 0.011526 | 0.020136 | 0.008778 | 0.020709 | 0.009702 | 0.014374 | 0.004664 | 0.013559 |
Eisosomes | 0.000597 | 0.000398 | 0.000486 | 0.000412 | 0.000716 | 0.001156 | 0.000221 | 0.000327 | 0.000672 | 0.000792 | 0.000379 | 0.000366 |
Endoplasmic Reticulum | 0.906432* | 0.932025* | 0.932203* | 0.819995* | 0.882859* | 0.819193* | 0.858093* | 0.725581* | 0.891625* | 0.873401* | 0.902301* | 0.768830* |
Endosome | 0.002524 | 0.003915 | 0.002050 | 0.007868 | 0.003637 | 0.008201 | 0.008694 | 0.015824 | 0.003223 | 0.006142 | 0.004731 | 0.012179 |
Golgi | 0.006571 | 0.004716 | 0.001493 | 0.006291 | 0.007018 | 0.020533 | 0.003795 | 0.007857 | 0.006852 | 0.012934 | 0.002422 | 0.007140 |
Lipid Particles | 0.008724 | 0.007233 | 0.006605 | 0.043355 | 0.012549 | 0.024461 | 0.014745 | 0.051176 | 0.011127 | 0.016185 | 0.009889 | 0.047593 |
Mitochondria | 0.002835 | 0.002306 | 0.001906 | 0.002170 | 0.004868 | 0.006433 | 0.000750 | 0.001645 | 0.004112 | 0.004451 | 0.001439 | 0.001886 |
Mitotic Spindle | 0.000116 | 0.000803 | 0.000099 | 0.001086 | 0.000047 | 0.000168 | 0.000845 | 0.004347 | 0.000073 | 0.000473 | 0.000400 | 0.002853 |
None | 0.002266 | 0.002847 | 0.004804 | 0.000584 | 0.003508 | 0.003451 | 0.001236 | 0.001804 | 0.003046 | 0.003161 | 0.003364 | 0.001245 |
Nuclear Periphery | 0.000174 | 0.000102 | 0.000144 | 0.000613 | 0.000106 | 0.000673 | 0.000976 | 0.001190 | 0.000132 | 0.000399 | 0.000479 | 0.000926 |
Nuclear Periphery Foci | 0.002088 | 0.000755 | 0.000621 | 0.003576 | 0.000733 | 0.003919 | 0.015703 | 0.015532 | 0.001237 | 0.002399 | 0.006706 | 0.010056 |
Nucleolus | 0.000026 | 0.000019 | 0.000010 | 0.000028 | 0.000067 | 0.000074 | 0.000017 | 0.000079 | 0.000052 | 0.000048 | 0.000013 | 0.000056 |
Nucleus | 0.000765 | 0.000307 | 0.000565 | 0.000892 | 0.000563 | 0.002180 | 0.002685 | 0.005132 | 0.000638 | 0.001280 | 0.001420 | 0.003190 |
Peroxisomes | 0.004812 | 0.004376 | 0.002376 | 0.002240 | 0.011186 | 0.006985 | 0.000395 | 0.001904 | 0.008816 | 0.005732 | 0.001577 | 0.002058 |
Vacuole | 0.001126 | 0.001021 | 0.002163 | 0.017214 | 0.001492 | 0.003657 | 0.011077 | 0.028846 | 0.001356 | 0.002390 | 0.005760 | 0.023517 |
Vacuole Periphery | 0.000440 | 0.000689 | 0.000192 | 0.002284 | 0.001067 | 0.002121 | 0.000634 | 0.002152 | 0.000834 | 0.001433 | 0.000370 | 0.002213 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.58 | -1.75 | -4.03 | -2.76 | -2.86 | -3.50 | -5.80 | -5.66 | -1.51 | 0.67 | -3.84 | -5.67 | -6.85 | -3.02 | -2.04 |
Bud Neck | -0.55 | -1.33 | -5.99 | -5.33 | -5.35 | -2.95 | -3.86 | -6.91 | -5.45 | -3.68 | -2.57 | -3.74 | -8.60 | -7.15 | -6.13 |
Bud Site | -1.08 | -1.34 | -6.59 | -6.16 | -5.89 | -2.63 | -6.36 | -8.60 | -7.44 | -3.11 | -2.56 | -5.68 | -10.79 | -9.65 | -6.82 |
Cell Periphery | 1.50 | -0.43 | -9.75 | -11.04 | -10.00 | 0.43 | 3.10 | -5.31 | -5.61 | -7.81 | 1.38 | 1.48 | -10.82 | -12.04 | -12.28 |
Cytoplasm | 8.64 | 5.12 | 3.90 | -4.37 | -0.60 | -3.84 | 0.26 | -4.55 | -1.35 | -4.36 | 2.12 | 4.38 | -1.59 | -3.37 | -5.24 |
Cytoplasmic Foci | -1.07 | 4.08 | 1.12 | 2.41 | -3.41 | -4.53 | 1.62 | -4.63 | -0.65 | -5.95 | -3.80 | 5.13 | -3.22 | 0.19 | -7.75 |
Eisosomes | 1.43 | 0.75 | 1.59 | -0.20 | 0.63 | -2.03 | 4.34 | 3.37 | 4.35 | -2.24 | -0.90 | 2.90 | 3.62 | 3.82 | 0.18 |
Endoplasmic Reticulum | -3.65 | -3.87 | 9.20 | 13.28 | 13.81 | 7.60 | 3.49 | 15.99 | 9.24 | 12.21 | 3.27 | -1.67 | 17.80 | 15.11 | 19.71 |
Endosome | -2.58 | 0.97 | -7.32 | -6.31 | -9.98 | -6.71 | -6.69 | -11.34 | -6.50 | -5.74 | -6.80 | -3.96 | -13.33 | -8.61 | -10.75 |
Golgi | 1.54 | 5.33 | 0.19 | -1.24 | -4.55 | -7.75 | 4.07 | -0.75 | 6.76 | -3.88 | -5.67 | 7.51 | -0.35 | 4.97 | -6.40 |
Lipid Particles | 1.04 | 1.52 | -9.73 | -10.36 | -10.61 | -5.78 | -1.30 | -11.14 | -7.27 | -9.93 | -3.92 | 0.96 | -14.62 | -12.27 | -15.08 |
Mitochondria | 0.40 | 0.72 | 0.48 | 0.21 | -0.46 | -1.63 | 5.85 | 4.35 | 6.59 | -2.99 | -0.46 | 4.08 | 3.17 | 5.24 | -1.42 |
Mitotic Spindle | -1.45 | 0.25 | -1.41 | -0.34 | -1.45 | -4.13 | -3.49 | -4.63 | -4.51 | -3.55 | -1.77 | -3.37 | -4.75 | -3.93 | -4.06 |
None | -0.55 | -2.52 | 2.15 | 3.10 | 6.49 | 0.05 | 2.59 | 1.80 | 1.95 | -0.89 | -0.15 | -0.44 | 2.80 | 3.37 | 4.31 |
Nuclear Periphery | 2.65 | 1.21 | -7.63 | -9.41 | -8.80 | -4.04 | -5.66 | -6.49 | -2.47 | -0.92 | -3.61 | -5.50 | -8.38 | -4.50 | -3.90 |
Nuclear Periphery Foci | 2.63 | 2.99 | -2.31 | -6.06 | -6.58 | -6.10 | -8.95 | -9.67 | -7.39 | -0.27 | -3.45 | -7.71 | -10.03 | -8.61 | -3.34 |
Nucleolus | 0.38 | 1.05 | -0.03 | -0.67 | -4.33 | -0.12 | 0.97 | -0.34 | -0.68 | -5.81 | 0.14 | 1.22 | -0.19 | -1.05 | -7.06 |
Nucleus | 2.39 | 1.04 | -1.05 | -4.06 | -2.60 | -5.11 | -6.12 | -7.19 | -4.44 | -3.50 | -3.51 | -4.90 | -7.23 | -5.26 | -4.85 |
Peroxisomes | 0.54 | 3.37 | 3.61 | 3.70 | 0.33 | 3.58 | 11.37 | 9.32 | 6.71 | -4.80 | 3.98 | 10.75 | 9.94 | 7.62 | -1.61 |
Vacuole | 0.52 | -3.95 | -9.37 | -9.45 | -8.73 | -4.03 | -9.45 | -13.36 | -12.22 | -7.91 | -3.36 | -9.74 | -16.21 | -15.39 | -12.45 |
Vacuole Periphery | -1.69 | 2.36 | -4.00 | -3.46 | -4.63 | -2.63 | 2.18 | -2.35 | -0.08 | -3.34 | -2.67 | 3.97 | -4.18 | -2.13 | -5.78 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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