Standard name
Human Ortholog
Description Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.98 0.95 0.95 0.89 0.87 0.9 0.79 0.75 0.71 0.76 0.96 0.99 0.99 0.92 0.87 0.95 0.96 0.93 0.92 0.97 0.94 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0 0.11 0.11 0.2 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0 0 0.06 0.06 0 0 0 0 0.12 0.22 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 3 2 0 0 0 0 0 0 0 0 0 0 0 4
Bud 0 0 1 0 0 0 0 1 1 0 1 1 0 0 0 0 1 0 0 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 2 2 4 0 5 1 9 7 1 1 1 2 2 1 0 0 0 0 0 0
Cytoplasm 287 173 121 116 116 127 131 142 119 73 100 138 288 351 488 347 224 209 298 164 118 221 287 273
Endoplasmic Reticulum 2 1 2 1 1 6 5 5 5 6 1 8 3 4 2 9 6 6 0 0 1 0 5 3
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 1 0 0
Mitochondria 1 1 0 2 0 7 8 5 17 11 28 25 2 0 0 1 1 2 1 0 0 0 1 2
Nucleus 4 0 0 1 1 2 1 4 9 4 4 7 0 1 1 6 3 1 2 4 2 0 1 5
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 8 2 2 4 5 3 10 5 6 6 8 4 8 3 1 44 56 18 2 1 1 0 2 9
Unique Cell Count 299 176 124 122 122 142 151 157 150 97 140 181 299 356 491 378 258 221 312 177 129 228 307 314
Labelled Cell Count 303 177 126 124 125 147 161 162 163 101 154 193 302 361 493 410 294 238 312 177 129 228 307 314


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.1 15.7 11.5 11.6 12.7 11.5 9.6 10.8 10.6 10.6 9.8 10.2 19.5 18.4 17.2 12.7 15.7 17.6 15.7 14.9 15.5
Std Deviation (1e-4) 2.4 3.2 2.1 2.5 3.0 2.7 2.5 2.2 2.1 2.1 2.2 2.1 3.2 3.2 2.9 2.5 3.4 3.1 3.1 3.1 3.7
Intensity Change (Log2) – – – 0.02 0.15 0.01 -0.26 -0.08 -0.11 -0.11 -0.22 -0.17 0.77 0.69 0.59 0.15 0.46 0.62 0.46 0.38 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -1.0 -2.6 -3.2 -2.4 -4.6 -5.0 -5.7 -5.1 -0.7 0.8 1.8 -2.2 -3.3 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.9 0 5.3 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.0 0 0 0 0 0 0 0 0 3.4 5.1 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.6423 28.9771 26.1507 25.7604 23.4439 25.6425 19.5181 25.26 22.6803 21.3166 20.8206 22.6692 23.535 30.8021 27.1421 25.4709 28.8742 27.4701
Actin 0.0042 0.0001 0.0175 0.0001 0.009 0.0096 0.0029 0.0002 0.0024 0.0002 0.005 0.0001 0.0251 0.0002 0.0154 0.0002 0.0001 0.0075
Bud 0.0022 0.0001 0.0007 0.0001 0.0002 0.0001 0.0023 0 0.0004 0 0.0001 0 0.0007 0 0.0012 0.0001 0 0.0001
Bud Neck 0.001 0.0002 0.0004 0.0003 0.0014 0.0014 0.0009 0.0001 0.0004 0.0003 0.0004 0.0005 0.001 0.0001 0.0006 0.0003 0.0002 0.0008
Bud Periphery 0.0013 0.0001 0.0006 0 0.0003 0.0001 0.0009 0 0.0005 0 0 0 0.0006 0 0.0007 0 0 0
Bud Site 0.0008 0.0001 0.0022 0 0.0003 0.0001 0.0016 0 0.0004 0 0.0004 0 0.0006 0 0.0006 0 0 0.0001
Cell Periphery 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.825 0.9453 0.8525 0.9072 0.8159 0.8025 0.8419 0.9807 0.9313 0.96 0.8266 0.9256 0.8011 0.9592 0.8699 0.9036 0.863 0.8107
Cytoplasmic Foci 0.0125 0.0004 0.0144 0.0002 0.001 0.0039 0.0185 0.0003 0.0043 0.0005 0.0197 0.0003 0.0166 0.0002 0.0091 0.0609 0.0001 0.0007
Eisosomes 0.0001 0 0.0001 0 0.0003 0.0001 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0046 0.0005 0.0011 0.0003 0.0092 0.0031 0.0061 0.0002 0.0014 0.0005 0.0004 0.0005 0.0027 0.0002 0.0004 0.0003 0.0002 0.001
Endosome 0.0086 0.0005 0.0056 0.0001 0.0028 0.0048 0.017 0.0001 0.0058 0.0002 0.0008 0.0002 0.0061 0 0.0033 0.0005 0 0.0006
Golgi 0.0018 0.0001 0.0025 0 0.0015 0.0035 0.0072 0 0.0045 0 0.0034 0 0.0044 0 0.0024 0.0002 0 0.0011
Lipid Particles 0.0038 0.0007 0.0069 0 0.005 0.01 0.0026 0 0.0008 0 0.001 0 0.0148 0 0.0067 0.0016 0 0.0007
Mitochondria 0.0107 0.0034 0.0022 0.0001 0.0186 0.0024 0.0038 0.0001 0.002 0.0001 0.0005 0.0001 0.0029 0.0001 0.0042 0.0001 0.0001 0.0003
None 0.0066 0.0002 0.0005 0.0002 0.001 0.0025 0.004 0.0003 0.001 0.0003 0.0002 0.0002 0.0018 0.0002 0.0019 0.0004 0.0001 0.0003
Nuclear Periphery 0.0172 0.0041 0.0056 0.0049 0.0223 0.0205 0.0088 0.0014 0.0027 0.003 0.0189 0.0052 0.0168 0.0028 0.0039 0.0027 0.0066 0.0126
Nucleolus 0.0006 0.0004 0.0006 0 0.0002 0.0003 0.0002 0 0.0001 0 0 0 0.0011 0 0.0004 0 0 0.0001
Nucleus 0.0834 0.0415 0.0803 0.086 0.1065 0.129 0.0588 0.0163 0.0397 0.0341 0.0689 0.0667 0.0815 0.0365 0.0649 0.0255 0.1291 0.1616
Peroxisomes 0.0035 0.0002 0.0019 0 0.0003 0.0014 0.0175 0 0.0006 0 0.0526 0 0.0128 0 0.0106 0.001 0 0.0003
Punctate Nuclear 0.0073 0.0002 0.0022 0.0004 0.0006 0.0029 0.0016 0.0002 0.0007 0.0005 0.0005 0.0004 0.0082 0.0003 0.0021 0.0023 0.0002 0.0009
Vacuole 0.0034 0.0012 0.0018 0.0002 0.0016 0.0012 0.0027 0.0001 0.0009 0.0001 0.0001 0.0003 0.0008 0.0001 0.0011 0.0001 0.0001 0.0004
Vacuole Periphery 0.0013 0.0007 0.0005 0.0001 0.0018 0.0007 0.0009 0 0.0003 0.0001 0.0002 0.0001 0.0005 0 0.0004 0 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 108.363 102.5266 96.0171 89.9287 114.3464 88.5397 97.4089 86.1312 107.299 101.0509
Translational Efficiency 0.6835 0.7275 0.7832 0.8977 0.6936 0.772 0.6465 0.8321 0.7405 0.6923

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1999 1592 124 640 1519 1583 202 373 3518 3175 326 1013

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1181.40 1395.84 1987.12 1486.73 1351.38 1471.36 1659.79 1545.32 1254.79 1433.49 1784.30 1508.30
Standard Deviation 174.29 275.68 232.63 216.07 189.12 260.10 218.34 229.38 199.48 270.67 274.55 222.86
Intensity Change Log 2 — 0.240636 0.750181 0.331645 — 0.122717 0.296567 0.193472 — 0.178921 0.525953 0.259572

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000106 0.000143 0.001370 0.002122 0.000188 0.000304 0.000447 0.000873 0.000141 0.000223 0.000798 0.001662
Bud Neck 0.000784 0.000511 0.005128 0.002756 0.000394 0.000785 0.002459 0.003875 0.000616 0.000647 0.003474 0.003168
Bud Site 0.001731 0.002125 0.005480 0.008671 0.001250 0.003944 0.003294 0.049453 0.001524 0.003032 0.004126 0.023687
Cell Periphery 0.000051 0.000044 0.000789 0.000294 0.000075 0.000049 0.000159 0.000233 0.000061 0.000046 0.000399 0.000272
Cytoplasm 0.816253* 0.721587* 0.457698* 0.500904* 0.767582* 0.764717* 0.490012* 0.550749* 0.795238* 0.743091* 0.477721* 0.519258*
Cytoplasmic Foci 0.013747 0.003782 0.016143 0.007282 0.009039 0.010104 0.006148 0.015362 0.011714 0.006934 0.009950 0.010257
Eisosomes 0.000004 0.000002 0.000011 0.000014 0.000004 0.000015 0.000016 0.000011 0.000004 0.000008 0.000014 0.000013
Endoplasmic Reticulum 0.000643 0.000382 0.001012 0.000817 0.001083 0.000332 0.014220 0.000825 0.000833 0.000357 0.009196 0.000819
Endosome 0.000652 0.000180 0.001325 0.001218 0.000602 0.001496 0.002420 0.014221 0.000630 0.000836 0.002004 0.006006
Golgi 0.000733 0.000178 0.001072 0.000452 0.000175 0.002210 0.000218 0.004407 0.000492 0.001191 0.000543 0.001908
Lipid Particles 0.000160 0.000105 0.002547 0.001953 0.000244 0.000443 0.001688 0.001122 0.000196 0.000274 0.002015 0.001647
Mitochondria 0.000423 0.000121 0.000397 0.000331 0.000089 0.001213 0.000398 0.000602 0.000279 0.000665 0.000398 0.000431
Mitotic Spindle 0.000859 0.002014 0.004505 0.024222 0.000447 0.006812 0.006239 0.035029 0.000681 0.004406 0.005579 0.028201
None 0.019798 0.014613 0.003567 0.015577 0.006902 0.010936 0.009342 0.014664 0.014230 0.012780 0.007146 0.015241
Nuclear Periphery 0.000104 0.000111 0.000958 0.000703 0.000206 0.000124 0.002424 0.000630 0.000148 0.000118 0.001866 0.000676
Nuclear Periphery Foci 0.003057 0.001388 0.006552 0.003543 0.004034 0.001691 0.009857 0.002748 0.003479 0.001539 0.008600 0.003250
Nucleolus 0.000206 0.000123 0.000206 0.000132 0.000080 0.000263 0.000206 0.000207 0.000151 0.000193 0.000206 0.000160
Nucleus 0.130090* 0.247317* 0.442895* 0.375325* 0.194667* 0.183287* 0.297288* 0.252404* 0.157973* 0.215393* 0.352672* 0.330064*
Peroxisomes 0.000116 0.000090 0.000108 0.001763 0.000032 0.000877 0.000084 0.000237 0.000080 0.000482 0.000093 0.001201
Vacuole 0.010141 0.005071 0.047985 0.051862 0.012788 0.008876 0.152760 0.050221 0.011284 0.006968 0.112907 0.051258
Vacuole Periphery 0.000341 0.000115 0.000254 0.000059 0.000120 0.001520 0.000320 0.002129 0.000246 0.000816 0.000295 0.000821

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.13 -8.40 -5.25 -4.96 3.77 -1.58 -7.77 -4.67 -1.48 3.06 -2.35 -10.17 -5.91 -4.89 2.79
Bud Neck 0.49 -8.51 -8.49 -13.86 4.91 -2.78 -9.58 -10.89 -8.88 1.82 -0.33 -11.51 -12.76 -16.37 4.43
Bud Site -0.61 -7.17 -7.58 -5.83 1.41 -2.65 -7.22 -3.09 -2.13 -1.17 -2.32 -9.71 -6.30 -4.65 -0.72
Cell Periphery 0.32 -9.54 -9.51 -9.55 6.52 4.08 -6.87 -6.97 -8.55 2.80 3.00 -10.42 -10.89 -11.37 5.44
Cytoplasm 16.81 25.16 46.27 32.79 -1.26 1.41 26.06 27.31 26.54 -3.26 13.26 36.49 52.26 43.16 -3.65
Cytoplasmic Foci 11.91 -3.12 3.41 -6.90 4.65 -0.39 2.11 0.47 0.69 -1.07 6.83 -0.73 2.86 -3.24 2.56
Eisosomes 4.63 -8.12 -14.23 -15.81 -1.52 -0.89 -11.90 -12.75 -0.69 3.91 -0.67 -14.40 -18.12 -3.35 1.36
Endoplasmic Reticulum 1.03 -3.42 -1.96 -5.11 2.61 5.58 -1.59 3.14 -4.74 1.85 3.29 -1.98 -0.57 -7.03 1.92
Endosome 2.92 -5.08 -0.83 -5.34 4.92 -1.76 -6.56 -1.21 -0.54 0.51 -0.25 -7.94 -1.42 -1.21 2.81
Golgi 1.64 -1.85 1.50 -0.86 8.54 -2.82 -4.65 -1.09 2.02 -0.62 -1.41 -1.16 0.77 2.14 3.24
Lipid Particles 0.74 -8.06 -11.97 -12.03 3.35 -1.39 -10.33 -11.80 -7.18 2.43 -1.37 -12.26 -15.42 -13.01 3.34
Mitochondria 1.47 -0.79 -0.38 -14.82 1.57 -2.15 -10.31 -6.50 1.04 -0.14 -1.19 -2.37 -2.05 0.21 0.89
Mitotic Spindle -1.60 -2.35 -5.50 -4.68 -2.85 -3.87 -3.23 -5.49 -4.08 -2.54 -4.05 -3.81 -7.77 -5.92 -3.04
None 3.97 13.87 6.14 3.15 -8.70 -4.57 0.27 -4.34 -1.82 -3.94 2.24 9.55 2.63 0.91 -6.77
Nuclear Periphery -1.44 -12.18 -19.06 -19.06 1.77 3.95 -11.21 -14.72 -15.39 2.61 0.97 -15.00 -23.80 -24.28 3.87
Nuclear Periphery Foci 5.03 -5.59 -0.91 -3.78 4.78 6.72 -0.76 5.60 -0.70 2.95 8.12 -4.07 1.35 -3.70 4.49
Nucleolus 1.56 -1.21 -0.09 -3.03 2.60 -1.93 -7.03 -10.59 -0.38 -0.17 -0.57 -3.58 -2.82 -1.18 1.62
Nucleus -22.03 -16.67 -24.13 -9.13 4.70 0.81 -7.26 -5.80 -6.29 2.75 -15.81 -15.93 -21.53 -12.18 3.50
Peroxisomes 0.57 -1.09 -1.14 -1.36 -0.84 -2.14 -6.14 -4.54 1.93 1.91 -1.93 -2.24 -1.39 0.73 -0.80
Vacuole 3.83 -8.66 -21.38 -21.94 -5.27 -0.03 -14.44 -17.87 -17.87 1.20 1.95 -15.71 -27.59 -27.87 0.19
Vacuole Periphery 1.39 0.12 1.61 0.82 7.77 -1.96 -2.41 -0.87 1.69 -0.28 -1.42 -0.02 1.00 1.84 1.95
Standard name
Human Ortholog
Description Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ptc2

Ptc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available