Standard name | |
---|---|
Human Ortholog | |
Description | Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 7 | 2 | 2 | 4 |
Bud | 3 | 3 | 8 | 7 | 10 | 11 | 40 | 16 | 22 | 27 | 39 | 33 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 2 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 2 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 5 | 1 | 3 | 3 | 7 | 6 | 3 | 2 | 8 | 11 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 80 | 301 | 622 | 351 | 510 | 624 | 687 | 675 | 467 | 555 | 445 | 518 | 437 | 428 | 339 | 238 | 146 | 130 | 112 | 297 | 509 | 300 | 316 | 362 |
Endoplasmic Reticulum | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 3 | 2 | 8 | 2 | 2 | 5 | 7 | 9 | 8 | 6 | 1 | 3 | 0 | 17 | 0 | 2 | 2 |
Endosome | 0 | 0 | 12 | 17 | 22 | 6 | 13 | 30 | 9 | 2 | 2 | 6 | 6 | 30 | 26 | 1 | 6 | 0 | 2 | 4 | 33 | 21 | 15 | 18 |
Golgi | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 2 |
Mitochondria | 7 | 3 | 7 | 23 | 70 | 214 | 358 | 310 | 179 | 362 | 337 | 372 | 0 | 0 | 0 | 3 | 6 | 0 | 1 | 1 | 23 | 5 | 3 | 6 |
Nucleus | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 3 | 5 | 1 | 0 | 3 | 2 | 1 | 1 | 3 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 2 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 6 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 2 | 0 | 1 | 2 | 2 | 3 | 2 | 0 | 2 | 0 | 4 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 3 | 3 |
Vac/Vac Membrane | 3 | 0 | 10 | 3 | 22 | 24 | 46 | 51 | 31 | 62 | 68 | 80 | 38 | 110 | 82 | 16 | 10 | 5 | 7 | 3 | 25 | 41 | 27 | 20 |
Unique Cell Count | 84 | 308 | 643 | 379 | 575 | 719 | 913 | 912 | 597 | 807 | 689 | 787 | 468 | 525 | 418 | 253 | 163 | 135 | 136 | 317 | 646 | 389 | 389 | 440 |
Labelled Cell Count | 94 | 312 | 668 | 406 | 645 | 885 | 1158 | 1103 | 723 | 1025 | 903 | 1030 | 489 | 585 | 460 | 272 | 180 | 138 | 136 | 317 | 646 | 389 | 389 | 440 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 5.6 | 5.2 | 5.0 | 4.9 | 4.3 | 4.2 | 4.3 | 4.3 | 4.2 | 4.1 | 4.1 | 6.6 | 6.9 | 6.6 | 7.6 | 7.6 | 7.2 | 6.5 | 6.7 | 7.4 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.8 | 1.6 | 1.4 | 0.9 | 1.0 | 1.1 | 1.1 | 1.1 | 1.0 | 1.0 | 1.0 | 1.0 | 0.9 | 1.9 | 2.2 | 1.4 | 1.3 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.07 | -0.28 | -0.3 | -0.27 | -0.27 | -0.3 | -0.33 | -0.32 | 0.34 | 0.4 | 0.35 | 0.55 | 0.56 | 0.47 | 0.32 | 0.36 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.8 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.0 | -5.5 | -6.6 | -11.4 | -11.9 | -10.0 | -13.5 | -14.7 | -14.5 | -2.6 | -8.6 | -8.5 | -1.8 | -3.9 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 2.4 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.8 | 3.5 | 3.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.6 | 7.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.5 | 2.1 | 3.6 | 4.0 | 3.6 | 5.3 | 6.5 | 6.7 | 5.3 | 10.9 | 10.2 | 3.8 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.511 | 4.3329 | 3.6029 | 3.6975 | 3.1042 | 3.8516 | 1.2529 | 1.4495 | 1.4434 | 0.9937 | 1.4494 | 1.1147 | 0.8699 | 1.0129 | 0.8023 | 0.9659 | 0.7622 | 0.5887 |
Actin | 0.009 | 0.0007 | 0.0287 | 0.0224 | 0.0315 | 0.0016 | 0.0447 | 0.0026 | 0.0155 | 0.0065 | 0.0676 | 0.0013 | 0.0314 | 0.0066 | 0.0281 | 0.0135 | 0.0114 | 0.0046 |
Bud | 0.0009 | 0.0005 | 0.004 | 0.0015 | 0.0016 | 0.0003 | 0.0015 | 0.0007 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0057 | 0.0016 | 0.0121 | 0.0037 | 0.0003 |
Bud Neck | 0.0122 | 0.0002 | 0.0048 | 0.0006 | 0.0034 | 0.0002 | 0.0019 | 0.0002 | 0.0007 | 0.0015 | 0.0002 | 0.0008 | 0.0004 | 0.0002 | 0.0023 | 0.001 | 0.0003 | 0.0009 |
Bud Periphery | 0.003 | 0.0004 | 0.0063 | 0.0026 | 0.0015 | 0.001 | 0.0023 | 0.0007 | 0.0003 | 0.0004 | 0.0006 | 0.0002 | 0.0003 | 0.0058 | 0.0016 | 0.0101 | 0.0039 | 0.0003 |
Bud Site | 0.0059 | 0.0098 | 0.0071 | 0.0096 | 0.0103 | 0.0001 | 0.0065 | 0.0037 | 0.0054 | 0.0024 | 0.0007 | 0.0002 | 0.0015 | 0.0064 | 0.0112 | 0.0042 | 0.0056 | 0.0002 |
Cell Periphery | 0.0004 | 0.0006 | 0.0011 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.4417 | 0.5276 | 0.4739 | 0.4974 | 0.4861 | 0.6663 | 0.3307 | 0.7334 | 0.6567 | 0.5432 | 0.8295 | 0.7852 | 0.3883 | 0.6941 | 0.6599 | 0.5108 | 0.6889 | 0.6829 |
Cytoplasmic Foci | 0.0324 | 0.0187 | 0.031 | 0.0206 | 0.0599 | 0.0127 | 0.0952 | 0.0128 | 0.0204 | 0.1213 | 0.0097 | 0.0272 | 0.0271 | 0.011 | 0.0164 | 0.036 | 0.0418 | 0.0193 |
Eisosomes | 0.0001 | 0.0001 | 0.0007 | 0.0016 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0037 | 0.0047 | 0.0049 | 0.0058 | 0.0026 | 0.0038 | 0.0095 | 0.0045 | 0.0048 | 0.0042 | 0.0068 | 0.011 | 0.0043 | 0.0056 | 0.0029 | 0.0025 | 0.0078 | 0.003 |
Endosome | 0.0085 | 0.0193 | 0.0219 | 0.0254 | 0.0349 | 0.0096 | 0.0929 | 0.0056 | 0.016 | 0.0403 | 0.0068 | 0.0101 | 0.0222 | 0.013 | 0.0116 | 0.0516 | 0.0527 | 0.0161 |
Golgi | 0.0017 | 0.0024 | 0.0042 | 0.0038 | 0.0105 | 0.0006 | 0.0222 | 0.0038 | 0.004 | 0.0251 | 0.0051 | 0.0019 | 0.0153 | 0.0004 | 0.0055 | 0.0165 | 0.0044 | 0.0015 |
Lipid Particles | 0.0044 | 0.0019 | 0.0024 | 0.0049 | 0.0275 | 0.0011 | 0.0606 | 0.0008 | 0.0086 | 0.0256 | 0.0008 | 0.0025 | 0.02 | 0.0004 | 0.0054 | 0.0163 | 0.0011 | 0.0008 |
Mitochondria | 0.0017 | 0.0017 | 0.0034 | 0.0348 | 0.0027 | 0.001 | 0.0155 | 0.0004 | 0.0056 | 0.0192 | 0.0139 | 0.0003 | 0.0071 | 0.001 | 0.0144 | 0.0076 | 0.004 | 0.001 |
None | 0.4508 | 0.4042 | 0.3829 | 0.3221 | 0.3013 | 0.2968 | 0.1837 | 0.2232 | 0.2288 | 0.1394 | 0.0352 | 0.1461 | 0.4307 | 0.2424 | 0.2257 | 0.2823 | 0.1449 | 0.2605 |
Nuclear Periphery | 0.0007 | 0.0009 | 0.0017 | 0.0061 | 0.0009 | 0.0006 | 0.0258 | 0.001 | 0.0013 | 0.001 | 0.0016 | 0.0013 | 0.019 | 0.0011 | 0.0009 | 0.0017 | 0.0041 | 0.0014 |
Nucleolus | 0.0005 | 0.0002 | 0.0007 | 0.0015 | 0.0008 | 0.0001 | 0.0021 | 0.0002 | 0.0004 | 0.0006 | 0 | 0.0002 | 0.0021 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 |
Nucleus | 0.0016 | 0.0009 | 0.0024 | 0.0046 | 0.0013 | 0.0007 | 0.0042 | 0.0013 | 0.0011 | 0.001 | 0.0019 | 0.0016 | 0.0075 | 0.0015 | 0.0013 | 0.0027 | 0.0043 | 0.0019 |
Peroxisomes | 0.0138 | 0.0005 | 0.0078 | 0.0154 | 0.0143 | 0.0013 | 0.0379 | 0.0014 | 0.0244 | 0.0563 | 0.0164 | 0.0046 | 0.0113 | 0.0001 | 0.0066 | 0.0228 | 0.0056 | 0.0003 |
Punctate Nuclear | 0.0022 | 0.0004 | 0.0045 | 0.0061 | 0.0032 | 0.0005 | 0.0409 | 0.0008 | 0.0023 | 0.0037 | 0.0004 | 0.003 | 0.0055 | 0.0007 | 0.0009 | 0.0032 | 0.0028 | 0.0022 |
Vacuole | 0.0044 | 0.0038 | 0.0049 | 0.0105 | 0.0045 | 0.0014 | 0.0108 | 0.0026 | 0.0025 | 0.006 | 0.0018 | 0.0019 | 0.0035 | 0.0031 | 0.0028 | 0.0036 | 0.0109 | 0.0021 |
Vacuole Periphery | 0.0004 | 0.0007 | 0.0008 | 0.0022 | 0.0007 | 0.0002 | 0.0104 | 0.0002 | 0.0007 | 0.0018 | 0.0003 | 0.0003 | 0.0015 | 0.0004 | 0.0005 | 0.0009 | 0.0015 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.6935 | 7.6286 | 7.925 | 7.8201 | 10.5111 | 6.8232 | 7.3276 | 8.3295 | 7.8806 | 10.4803 |
Translational Efficiency | 2.1225 | 1.2159 | 1.1474 | 1.1359 | 0.8026 | 1.5716 | 1.8187 | 1.2887 | 1.6849 | 1.2691 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1961 | 1049 | 1702 | 1335 | 1690 | 1491 | 1366 | 2821 | 3651 | 2540 | 3068 | 4156 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 750.21 | 745.78 | 924.25 | 919.30 | 734.88 | 797.29 | 967.30 | 1014.63 | 743.11 | 776.02 | 943.42 | 984.01 |
Standard Deviation | 84.69 | 124.42 | 116.23 | 124.72 | 88.88 | 112.16 | 124.19 | 135.95 | 86.99 | 120.09 | 121.74 | 139.73 |
Intensity Change Log 2 | — | -0.008544 | 0.300989 | 0.293241 | — | 0.117596 | 0.396455 | 0.465373 | — | 0.055253 | 0.349019 | 0.380985 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000248 | 0.000420 | 0.000216 | 0.000536 | 0.000127 | 0.000200 | 0.000276 | 0.000382 | 0.000192 | 0.000291 | 0.000243 | 0.000431 |
Bud Neck | 0.015300 | 0.019578 | 0.004077 | 0.004278 | 0.007818 | 0.017961 | 0.003379 | 0.010302 | 0.011837 | 0.018629 | 0.003766 | 0.008367 |
Bud Site | 0.006095 | 0.021851 | 0.007107 | 0.029574 | 0.005704 | 0.017067 | 0.011484 | 0.030505 | 0.005914 | 0.019043 | 0.009056 | 0.030206 |
Cell Periphery | 0.000104 | 0.000132 | 0.000088 | 0.000156 | 0.000101 | 0.000118 | 0.000129 | 0.000050 | 0.000103 | 0.000124 | 0.000106 | 0.000084 |
Cytoplasm | 0.430225* | 0.413808* | 0.558426* | 0.555963* | 0.539930* | 0.426274* | 0.566514* | 0.680941* | 0.481006* | 0.421126* | 0.562027* | 0.640795* |
Cytoplasmic Foci | 0.285516* | 0.245159* | 0.015979 | 0.043530 | 0.221229* | 0.221288* | 0.021603 | 0.020035 | 0.255758* | 0.231147* | 0.018483 | 0.027582 |
Eisosomes | 0.000180 | 0.000087 | 0.000025 | 0.000043 | 0.000095 | 0.000075 | 0.000039 | 0.000012 | 0.000140 | 0.000080 | 0.000031 | 0.000022 |
Endoplasmic Reticulum | 0.002727 | 0.001731 | 0.002810 | 0.001786 | 0.002927 | 0.001304 | 0.002392 | 0.000331 | 0.002819 | 0.001480 | 0.002624 | 0.000798 |
Endosome | 0.036375 | 0.060250 | 0.002743 | 0.007824 | 0.018124 | 0.042288 | 0.005254 | 0.008860 | 0.027927 | 0.049706 | 0.003861 | 0.008527 |
Golgi | 0.006032 | 0.006105 | 0.000086 | 0.006061 | 0.003027 | 0.004041 | 0.000367 | 0.002611 | 0.004641 | 0.004894 | 0.000211 | 0.003719 |
Lipid Particles | 0.011104 | 0.007163 | 0.001177 | 0.002181 | 0.007447 | 0.006710 | 0.001937 | 0.000548 | 0.009411 | 0.006897 | 0.001515 | 0.001073 |
Mitochondria | 0.005025 | 0.004598 | 0.000507 | 0.001083 | 0.003277 | 0.006291 | 0.000507 | 0.003489 | 0.004216 | 0.005592 | 0.000507 | 0.002716 |
Mitotic Spindle | 0.002665 | 0.003499 | 0.006775 | 0.013404 | 0.002102 | 0.009166 | 0.002832 | 0.039682 | 0.002405 | 0.006825 | 0.005019 | 0.031241 |
None | 0.000640 | 0.002581 | 0.003540 | 0.001286 | 0.001309 | 0.001642 | 0.004565 | 0.002518 | 0.000950 | 0.002029 | 0.003996 | 0.002122 |
Nuclear Periphery | 0.000354 | 0.000467 | 0.001957 | 0.000586 | 0.000395 | 0.000533 | 0.001302 | 0.000528 | 0.000373 | 0.000506 | 0.001665 | 0.000547 |
Nuclear Periphery Foci | 0.000355 | 0.001356 | 0.002254 | 0.003563 | 0.000564 | 0.000455 | 0.004371 | 0.000345 | 0.000452 | 0.000827 | 0.003196 | 0.001379 |
Nucleolus | 0.000725 | 0.003553 | 0.000285 | 0.000193 | 0.000576 | 0.001238 | 0.000376 | 0.000432 | 0.000656 | 0.002195 | 0.000325 | 0.000356 |
Nucleus | 0.058012 | 0.042087 | 0.307190* | 0.070665 | 0.086674 | 0.054576 | 0.225378* | 0.053956 | 0.071279 | 0.049418 | 0.270764* | 0.059324 |
Peroxisomes | 0.003911 | 0.010198 | 0.000225 | 0.000982 | 0.003317 | 0.005155 | 0.000362 | 0.002506 | 0.003636 | 0.007238 | 0.000286 | 0.002017 |
Vacuole | 0.131603 | 0.150495 | 0.084356 | 0.254718* | 0.093532 | 0.179072 | 0.146621 | 0.139690 | 0.113980 | 0.167270 | 0.112079 | 0.176640 |
Vacuole Periphery | 0.002804 | 0.004883 | 0.000177 | 0.001588 | 0.001724 | 0.004545 | 0.000310 | 0.002275 | 0.002304 | 0.004685 | 0.000236 | 0.002054 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.04 | -0.54 | -2.00 | 0.28 | -2.77 | -2.46 | -4.61 | -2.75 | -0.20 | 2.13 | -2.16 | -2.24 | -1.80 | 0.64 | 0.75 |
Bud Neck | -2.32 | 9.02 | 11.68 | 9.74 | 1.42 | -7.25 | 6.36 | -0.08 | 7.27 | -6.66 | -5.97 | 10.78 | 8.31 | 11.37 | -3.82 |
Bud Site | -9.04 | -0.22 | -5.86 | 1.87 | -5.26 | -6.10 | -1.40 | -7.38 | 1.44 | -3.68 | -10.02 | -1.16 | -9.37 | 2.54 | -6.63 |
Cell Periphery | -1.36 | 1.17 | -0.62 | 1.06 | -1.54 | -1.51 | -1.49 | 18.09 | 5.38 | 9.41 | -1.99 | -0.35 | 11.81 | 5.45 | 8.96 |
Cytoplasm | 1.58 | -5.98 | -2.69 | -3.62 | 2.59 | 11.72 | 5.98 | -6.58 | -18.64 | -12.44 | 8.30 | -0.22 | -10.92 | -17.75 | -10.20 |
Cytoplasmic Foci | 5.27 | 57.61 | 52.13 | 31.93 | -9.67 | 0.15 | 42.68 | 43.03 | 40.02 | -0.10 | 4.97 | 70.59 | 68.62 | 51.49 | -6.01 |
Eisosomes | 4.27 | 6.94 | 6.59 | 7.83 | -3.30 | 3.41 | 9.44 | 16.68 | 18.92 | 18.16 | 5.08 | 8.76 | 10.26 | 18.79 | 12.64 |
Endoplasmic Reticulum | 2.65 | -3.01 | 3.12 | 0.09 | 10.56 | 3.49 | -0.34 | 5.86 | 6.13 | 18.78 | 4.59 | -2.31 | 7.64 | 5.23 | 22.42 |
Endosome | -6.66 | 21.25 | 19.54 | 17.74 | -2.48 | -10.96 | 14.32 | 13.22 | 18.46 | -1.27 | -10.75 | 25.22 | 23.12 | 25.39 | -3.18 |
Golgi | 0.13 | 12.31 | 3.98 | 3.62 | -2.14 | -1.37 | 6.61 | 4.59 | 5.13 | -2.47 | -0.27 | 13.78 | 7.44 | 6.71 | -3.25 |
Lipid Particles | 4.30 | 14.79 | 13.05 | 7.34 | -1.73 | 0.99 | 11.32 | 14.17 | 8.44 | 5.02 | 4.05 | 18.57 | 19.55 | 11.45 | 3.13 |
Mitochondria | 0.46 | 5.25 | 5.26 | 5.07 | 0.33 | -2.55 | 3.74 | 2.32 | 5.34 | -4.08 | -1.53 | 6.44 | 5.14 | 7.02 | -3.78 |
Mitotic Spindle | -0.58 | -3.57 | -3.87 | -3.46 | -0.85 | -3.75 | -1.22 | -9.32 | -4.58 | -8.99 | -3.58 | -3.74 | -10.06 | -6.08 | -7.03 |
None | -3.86 | -15.10 | -5.58 | 2.51 | 10.82 | -1.06 | -7.81 | -7.94 | -3.28 | 3.56 | -3.80 | -14.70 | -11.91 | -0.96 | 7.11 |
Nuclear Periphery | -1.38 | -19.48 | -6.24 | -3.95 | 11.99 | -1.96 | -21.59 | -13.12 | -7.10 | 8.96 | -2.50 | -26.61 | -14.60 | -8.36 | 14.83 |
Nuclear Periphery Foci | -6.36 | -13.35 | -14.02 | -6.99 | -1.12 | 1.83 | -9.68 | 1.53 | 0.01 | 9.90 | -5.26 | -14.94 | -10.97 | -4.70 | 10.24 |
Nucleolus | -4.23 | 1.52 | 2.58 | 5.28 | 2.49 | -3.23 | 1.72 | -1.48 | 2.79 | -3.91 | -5.01 | 2.00 | 1.05 | 5.79 | -2.22 |
Nucleus | 4.26 | -42.51 | -8.66 | -10.93 | 35.56 | 8.80 | -26.00 | -0.82 | -10.82 | 26.53 | 8.50 | -48.66 | -7.73 | -15.54 | 44.62 |
Peroxisomes | -6.70 | 13.43 | 10.99 | 10.67 | -2.79 | -2.20 | 6.32 | 4.14 | 5.72 | -3.50 | -5.95 | 12.70 | 8.64 | 11.17 | -4.30 |
Vacuole | -3.59 | 0.30 | -28.22 | -22.80 | -26.64 | -15.22 | -16.88 | -25.92 | -8.82 | -3.11 | -13.01 | -11.86 | -35.15 | -20.30 | -19.88 |
Vacuole Periphery | -3.70 | 17.32 | 8.06 | 7.50 | -1.85 | -6.94 | 9.70 | 3.41 | 8.59 | -3.00 | -7.17 | 19.38 | 8.03 | 11.26 | -3.61 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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