Standard name
Human Ortholog
Description Heme a synthase; required for the hydroxylation of heme O to form heme A, an essential prosthetic group for cytochrome c oxidase; oligomerization is required for function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.14 0 0 0 0 0.06 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0 0.12 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.12 0.42 0.42 0 0.13 0.08 0.06 0.11 0.08
Mitochondria 0.98 0.93 0.9 0.97 0.89 0.92 0.88 0.9 0.92 0.91 0.87 0.93 0.94 0.97 0.88 0.12 0.13 0.1 0.92 0.72 0.76 0.59 0.74 0.76
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.08 0.11 0.07 0.08 0.05 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0 0.09 0.11 0.08 0.08 0.2 0.15 0.14 0.14 0.16 0.17 0 0 0 0.18 0.06 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0.1 0.05 0 0 0 0 0 0 0.08 0.05 0.11 0.12 0.15 0.08 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 0 0 1 0 2 1 0 0 1 4 1 4 7 4 2 1 4 9 4
Bud 2 0 1 0 3 2 0 2 3 5 4 4 0 0 1 1 1 3 0 0 2 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 1 0 1 1 4 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 1 0 0 1 1 1 0 0 3 1 2 1 7 2 6 0 0 0 0 0 0
Cytoplasm 0 1 5 2 4 6 3 8 7 6 8 5 4 1 1 3 2 5 0 0 2 2 0 0
Endoplasmic Reticulum 0 1 1 0 1 1 1 0 0 1 1 1 1 1 2 3 1 2 0 1 0 0 0 1
Endosome 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 1 3 1 1 1 0 4 1 0
Golgi 0 3 2 2 6 1 1 2 0 0 2 2 2 15 17 4 22 21 4 16 8 6 16 14
Mitochondria 327 110 101 93 135 178 200 271 208 222 166 212 98 201 203 4 7 5 309 87 88 58 119 134
Nucleus 0 1 1 0 0 1 6 2 0 2 0 0 0 1 1 1 0 0 0 0 0 2 0 0
Nuclear Periphery 8 4 4 0 5 15 24 22 17 11 18 11 3 4 5 1 2 0 0 1 0 3 0 1
Nucleolus 36 5 10 11 12 16 46 44 32 34 30 39 1 5 7 6 3 3 1 1 1 1 1 2
Peroxisomes 1 0 0 0 0 1 0 1 2 0 1 1 0 2 1 3 4 3 7 1 4 3 4 4
SpindlePole 2 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 1 0 1 0 1 1 0 0
Vac/Vac Membrane 11 9 5 1 15 10 10 9 0 9 6 3 8 11 25 4 8 4 4 7 1 3 5 6
Unique Cell Count 333 118 112 96 152 194 226 301 225 244 190 229 104 207 232 34 53 50 337 122 117 99 161 177
Labelled Cell Count 389 135 131 110 183 232 292 365 271 296 240 285 118 244 268 39 60 60 337 122 117 99 161 177


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 18.0 14.9 14.4 16.9 16.9 15.4 15.7 16.2 15.5 14.3 15.8 18.8 19.6 21.2 17.3 20.5 20.8 11.7 17.0 20.4
Std Deviation (1e-4) 3.4 5.0 4.0 3.8 4.3 4.4 4.5 4.9 5.1 4.8 4.9 4.9 4.5 4.2 5.7 8.0 7.5 7.0 6.1 3.6 5.1
Intensity Change (Log2) – – – -0.04 0.18 0.19 0.05 0.08 0.12 0.06 -0.05 0.09 0.34 0.4 0.51 0.22 0.47 0.48 -0.35 0.19 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 2.1 2.1 0 6.8 6.8
Mitochondria 1.9 -0.4 0.5 -0.5 0 0.7 0.2 -0.7 0.8 1.1 2.6 -0.7 -8.9 -9.7 -9.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1.5 2.2 1.4 1.4 0 1.9 0 0 0 0 0 0 0
Nucleolus 0.6 -0.3 -0.2 2.7 1.5 1.4 1.3 1.7 2.0 -2.7 -2.6 -2.4 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.6 0.3 0 0 0 0 0 0 1.0 0.3 1.9 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.748 17.2575 16.5092 15.0206 15.7633 16.4676 12.6824 14.2727 12.9909 13.0194 12.3184 13.3314 14.539 16.5562 16.5887 13.918 16.9507 16.7285
Actin 0.014 0.0019 0.002 0.0001 0.0003 0.0029 0.0465 0.0027 0.0136 0.0025 0.0218 0.0044 0.0246 0.0269 0.0171 0.0146 0.0002 0.0075
Bud 0.0003 0.0001 0.0002 0 0 0.0002 0.0005 0 0.0001 0.0007 0.0001 0 0.0003 0.0001 0.0004 0.0005 0 0
Bud Neck 0.0002 0 0.0004 0 0 0.0001 0.0005 0.0001 0.0001 0 0.0001 0.0001 0.0013 0.0002 0.0004 0.0004 0 0.0001
Bud Periphery 0.0006 0.0001 0.0004 0 0.0001 0.0002 0.0011 0.0001 0.0005 0.0038 0.0004 0.0002 0.0004 0.0003 0.0006 0.0005 0.0002 0.0001
Bud Site 0.0011 0.0001 0.0054 0 0.0001 0.0001 0.0054 0.0001 0.0008 0.0001 0.0005 0 0.0044 0.0003 0.004 0.0035 0 0.0001
Cell Periphery 0.0003 0.0002 0.0004 0 0.0002 0.0001 0.0008 0.0001 0.002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0004 0.0001 0.0002 0.0001
Cytoplasm 0.0002 0 0.0002 0 0 0.0007 0.0001 0 0.0009 0 0.0001 0.0002 0.0028 0.0004 0.001 0.0036 0 0.0003
Cytoplasmic Foci 0.0083 0.0005 0.0012 0 0.0018 0.0008 0.0081 0.0011 0.003 0.0002 0.0011 0.0002 0.0337 0.0008 0.0098 0.0597 0 0.0024
Eisosomes 0.0004 0.0002 0.0003 0.0001 0.0001 0.0001 0.0015 0.0005 0.0009 0.0001 0.0006 0.0001 0.0003 0.0005 0.0003 0.0002 0 0.0004
Endoplasmic Reticulum 0.0002 0 0 0 0.0001 0.0002 0.0004 0 0.0002 0 0.0004 0.0008 0.0013 0.0004 0.0009 0.0011 0 0.0002
Endosome 0.0062 0.002 0.0044 0.0001 0.0019 0.003 0.0115 0.002 0.0023 0.0003 0.0024 0.0021 0.0223 0.0019 0.0088 0.0161 0.0003 0.0011
Golgi 0.0065 0.0049 0.0039 0.0003 0.0015 0.0027 0.0282 0.0114 0.0034 0.0009 0.005 0.004 0.0139 0.0035 0.0077 0.007 0.0001 0.0041
Lipid Particles 0.0135 0.0089 0.0025 0.0003 0.0116 0.0022 0.0348 0.0029 0.0042 0.0012 0.0048 0.0085 0.028 0.0067 0.0137 0.0195 0.0003 0.0076
Mitochondria 0.9253 0.9705 0.9698 0.9982 0.9628 0.9759 0.8383 0.9649 0.9462 0.9865 0.9582 0.9646 0.8257 0.9372 0.9159 0.7765 0.9843 0.9632
None 0.0004 0 0 0 0 0 0.0003 0 0.0003 0 0.0004 0.0001 0.004 0.0032 0.0005 0.0027 0 0.0065
Nuclear Periphery 0.001 0 0 0 0.0002 0.0003 0.0007 0 0.0019 0 0.0002 0.0063 0.0018 0.0015 0.0012 0.002 0 0.0004
Nucleolus 0.0032 0.0002 0.0003 0 0.0108 0 0.0024 0.0004 0.0001 0 0.0001 0.0001 0.0007 0.0007 0.0005 0.0007 0 0.0002
Nucleus 0.0007 0.0001 0.0001 0 0.0009 0.0001 0.0003 0.0001 0.0009 0 0.0001 0.001 0.0007 0.0009 0.0005 0.0024 0 0.0001
Peroxisomes 0.0048 0.0024 0.002 0.0004 0.0013 0.0019 0.0127 0.0035 0.0095 0.0025 0.0027 0.0006 0.0217 0.0053 0.006 0.0387 0.0005 0.0016
Punctate Nuclear 0.0014 0 0 0 0.0001 0.0001 0.0009 0 0.0033 0 0.0001 0.0001 0.0059 0.0041 0.0007 0.0068 0 0.0005
Vacuole 0.0025 0.0023 0.0027 0.0001 0.0019 0.0011 0.0012 0.0038 0.0018 0.0002 0.0002 0.0006 0.002 0.0013 0.003 0.0086 0.0043 0.0003
Vacuole Periphery 0.0091 0.0053 0.0038 0.0005 0.0041 0.0073 0.0039 0.0061 0.004 0.0005 0.0007 0.0059 0.0041 0.0034 0.0067 0.0348 0.0094 0.0032

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.6781 22.2795 23.5461 35.0453 34.7616 41.5152 36.5208 46.3034 53.0296 41.1561
Translational Efficiency 1.2779 1.6535 1.1941 1.2923 1.0968 0.9927 1.2796 0.9689 0.9081 0.9514

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1756 471 1975 1278 1735 1227 152 55 3491 1698 2127 1333

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1240.00 1463.84 2454.55 1821.26 1436.62 1402.56 2024.22 1829.62 1337.72 1419.56 2423.80 1821.60
Standard Deviation 309.25 449.04 574.75 435.05 329.70 336.65 415.12 482.62 334.36 372.26 575.61 437.12
Intensity Change Log 2 — 0.239418 0.985118 0.554597 — -0.034616 0.494688 0.348866 — 0.098828 0.742690 0.447832

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001557 0.010118 0.001789 0.006290 0.001081 0.006926 0.004093 0.017713 0.001320 0.007811 0.001954 0.006761
Bud Neck 0.001582 0.002538 0.001110 0.001277 0.000831 0.001658 0.005124 0.003720 0.001209 0.001902 0.001397 0.001378
Bud Site 0.006103 0.003147 0.000636 0.002933 0.000430 0.002909 0.003825 0.002295 0.003284 0.002975 0.000864 0.002907
Cell Periphery 0.000966 0.000594 0.000092 0.000754 0.000074 0.000159 0.000176 0.000466 0.000523 0.000280 0.000098 0.000743
Cytoplasm 0.000829 0.000464 0.000104 0.000308 0.000104 0.000577 0.000364 0.000321 0.000468 0.000546 0.000123 0.000309
Cytoplasmic Foci 0.003598 0.014851 0.014193 0.006382 0.009729 0.013531 0.031281 0.034477 0.006645 0.013897 0.015414 0.007541
Eisosomes 0.000575 0.001431 0.000710 0.000583 0.000588 0.000464 0.000419 0.000747 0.000581 0.000732 0.000689 0.000589
Endoplasmic Reticulum 0.000175 0.000311 0.000070 0.000492 0.000068 0.000315 0.000173 0.002202 0.000122 0.000313 0.000077 0.000562
Endosome 0.001836 0.005005 0.000724 0.003100 0.000978 0.004567 0.005055 0.009202 0.001410 0.004688 0.001034 0.003352
Golgi 0.004491 0.034229 0.015716 0.031812 0.007798 0.026094 0.053065 0.061014 0.006135 0.028350 0.018385 0.033017
Lipid Particles 0.002221 0.007753 0.008036 0.001778 0.002828 0.004506 0.006328 0.004065 0.002523 0.005407 0.007914 0.001873
Mitochondria 0.944254* 0.877335* 0.889655* 0.911070* 0.952339* 0.907101* 0.811548* 0.760370* 0.948272* 0.898844* 0.884073* 0.904852*
Mitotic Spindle 0.000253 0.003221 0.001620 0.002328 0.000231 0.001154 0.006557 0.003260 0.000242 0.001727 0.001973 0.002367
None 0.003070 0.002949 0.008144 0.002051 0.003032 0.001694 0.004057 0.003317 0.003051 0.002042 0.007852 0.002103
Nuclear Periphery 0.000235 0.000267 0.002520 0.000343 0.000314 0.000299 0.002219 0.002196 0.000274 0.000290 0.002498 0.000420
Nuclear Periphery Foci 0.000162 0.000407 0.000750 0.000174 0.000270 0.000296 0.000907 0.001402 0.000215 0.000327 0.000761 0.000225
Nucleolus 0.000615 0.001031 0.001420 0.000420 0.001030 0.000673 0.001286 0.002365 0.000821 0.000773 0.001411 0.000500
Nucleus 0.000177 0.000209 0.000079 0.000062 0.000113 0.000258 0.000138 0.000147 0.000145 0.000244 0.000083 0.000065
Peroxisomes 0.005002 0.014250 0.001516 0.003079 0.004865 0.006256 0.010766 0.006693 0.004934 0.008474 0.002177 0.003228
Vacuole 0.008892 0.010687 0.010053 0.007353 0.005397 0.010290 0.018154 0.034694 0.007155 0.010400 0.010632 0.008482
Vacuole Periphery 0.013408 0.009204 0.041063 0.017409 0.007902 0.010273 0.034465 0.049334 0.010671 0.009977 0.040591 0.018726

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.07 -0.43 -4.59 1.66 -4.18 -4.55 -3.01 -1.38 -0.88 -1.12 -5.91 -1.40 -5.03 0.71 -4.20
Bud Neck -1.40 1.16 0.61 1.99 -0.69 -2.23 -3.04 -1.67 -1.20 0.60 -2.01 -0.73 -0.62 1.37 -0.01
Bud Site 1.83 4.22 2.02 0.05 -3.10 -3.10 -2.47 -1.89 0.47 0.92 0.31 3.55 0.24 -0.05 -2.86
Cell Periphery 1.16 5.43 0.78 -0.46 -3.07 -4.04 -2.93 -2.29 -1.80 -1.68 2.16 5.18 -1.00 -2.10 -3.12
Cytoplasm 0.96 1.99 1.40 0.92 -3.45 -2.14 -3.24 -2.21 1.14 0.37 -0.34 1.88 0.81 1.36 -3.28
Cytoplasmic Foci -5.76 -8.72 -3.31 4.14 5.80 -2.39 -3.63 -1.92 -1.62 -0.21 -6.15 -6.99 -0.89 4.61 5.47
Eisosomes -4.60 -1.92 -0.08 4.39 1.46 1.76 2.24 -0.58 -1.05 -1.20 -2.13 -1.90 -0.10 1.52 1.19
Endoplasmic Reticulum -2.24 4.65 -2.45 -1.31 -3.30 -3.72 -3.26 -1.19 -1.05 -1.13 -3.73 3.33 -3.07 -1.64 -3.39
Endosome -2.28 3.63 -2.35 1.32 -4.99 -5.17 -2.72 -2.07 -1.15 -0.98 -5.16 1.61 -3.88 1.72 -4.66
Golgi -5.79 -7.13 -9.48 0.42 -5.24 -6.93 -3.86 -3.56 -2.32 -0.42 -9.48 -7.42 -9.60 -1.32 -4.70
Lipid Particles -2.60 -6.41 0.71 2.82 7.09 -2.27 -2.18 -1.06 0.34 1.17 -3.60 -6.85 1.34 4.16 7.24
Mitochondria 6.02 9.72 5.15 -2.84 -3.10 6.93 6.58 4.75 3.61 1.13 8.85 13.07 7.30 -0.78 -2.97
Mitotic Spindle -1.37 -4.43 -3.25 0.39 -0.99 -2.48 -3.05 -2.15 -1.45 1.31 -2.26 -5.34 -3.46 -0.71 -0.55
None 0.38 -14.78 5.40 3.04 19.39 6.90 -1.13 -0.48 -2.82 0.70 6.45 -15.43 6.30 -0.44 19.09
Nuclear Periphery -0.73 -8.64 -1.71 -1.16 8.09 0.19 -3.40 -2.34 -2.36 0.02 -0.30 -8.94 -2.08 -1.65 8.13
Nuclear Periphery Foci -2.28 -6.67 -0.39 2.10 6.31 -0.41 -3.22 -2.30 -2.24 -0.95 -2.09 -6.48 -0.27 1.67 6.04
Nucleolus -1.17 -2.32 0.79 1.59 2.68 0.90 -0.50 -0.83 -1.06 -0.69 0.20 -1.67 1.16 1.00 2.53
Nucleus -0.45 2.31 2.70 2.55 0.83 -1.67 -0.49 -0.52 1.13 -0.15 -1.53 2.05 2.61 2.96 0.90
Peroxisomes -2.68 3.98 1.69 3.25 -1.93 -1.06 -1.81 -0.57 -0.12 0.95 -2.63 4.25 1.83 3.64 -1.26
Vacuole -0.78 -1.05 1.07 1.36 2.05 -2.82 -3.26 -2.49 -2.07 -1.36 -2.32 -3.72 -1.07 1.08 1.54
Vacuole Periphery 2.37 -11.04 -1.98 -4.08 8.81 -1.66 -4.34 -3.78 -3.56 -1.22 0.59 -13.60 -4.55 -4.84 8.39
Standard name
Human Ortholog
Description Heme a synthase; required for the hydroxylation of heme O to form heme A, an essential prosthetic group for cytochrome c oxidase; oligomerization is required for function
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Cox15

Cox15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cox15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available