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Description | Subunit of cohesin loading factor (Scc2p-Scc4p); complex is required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | – | 1 | 3 | 1 | 1 | – | 0 | 3 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | – | 0 | 0 | 1 | 4 | – | 1 | 2 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 16 | – | 30 | 23 | 49 | 50 | – | 17 | 27 | 13 | 16 | 9 | 45 | 51 | 7 | 10 | 25 | 7 | 4 | 2 | 2 | 13 | 22 |
Endoplasmic Reticulum | 0 | 2 | – | 1 | 0 | 0 | 2 | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 4 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1 | – | 1 | 8 | 7 | 5 | – | 0 | 4 | 2 | 0 | 1 | 1 | 2 | 3 | 4 | 7 | 3 | 4 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 |
Mitochondria | 75 | 3 | – | 7 | 3 | 35 | 69 | – | 92 | 80 | 121 | 105 | 8 | 1 | 3 | 3 | 2 | 4 | 1 | 2 | 4 | 0 | 3 | 8 |
Nucleus | 160 | 97 | – | 96 | 77 | 84 | 156 | – | 85 | 132 | 84 | 148 | 29 | 106 | 89 | 43 | 34 | 46 | 168 | 118 | 25 | 18 | 64 | 68 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 3 | – | 0 | 2 | 2 | 3 | – | 2 | 5 | 4 | 4 | 0 | 0 | 0 | 1 | 1 | 0 | 9 | 3 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | – | 5 | 15 | 3 | 15 | – | 1 | 11 | 3 | 2 | 1 | 2 | 4 | 0 | 1 | 2 | 12 | 8 | 0 | 0 | 3 | 5 |
Vac/Vac Membrane | 23 | 45 | – | 7 | 26 | 32 | 21 | – | 12 | 10 | 11 | 8 | 4 | 5 | 11 | 11 | 18 | 13 | 44 | 25 | 24 | 8 | 41 | 67 |
Unique Cell Count | 223 | 147 | 141 | 145 | 185 | 272 | 163 | 215 | 170 | 217 | 43 | 154 | 151 | 68 | 62 | 97 | 256 | 174 | 64 | 31 | 135 | 183 | ||
Labelled Cell Count | 277 | 168 | 148 | 157 | 215 | 328 | 210 | 274 | 246 | 291 | 53 | 160 | 163 | 74 | 75 | 108 | 256 | 174 | 64 | 31 | 135 | 183 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 4.9 | – | 5.1 | 5.7 | 4.6 | 4.6 | – | 3.9 | 4.3 | 3.8 | 3.8 | 5.2 | 5.4 | 5.5 | 7.9 | 7.2 | 7.1 | – | 5.1 | 5.3 |
Std Deviation (1e-4) | 0.5 | 0.7 | – | 1.0 | 1.6 | 1.5 | 1.3 | – | 1.3 | 1.2 | 1.5 | 0.7 | 1.1 | 0.8 | 1.6 | 3.4 | 1.7 | 2.4 | – | 1.1 | 1.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |