Standard name
Description Subunit of cohesin loading factor (Scc2p-Scc4p); complex is required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.11 – 0.21 0.16 0.26 0.18 – 0.1 0.13 0.08 0.07 0.21 0.29 0.34 0.1 0.16 0.26 0 0 0 0.09 0.1 0.12
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 – 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.34 0 – 0.05 0 0.19 0.25 – 0.56 0.37 0.71 0.48 0.19 0 0 0 0 0 0 0 0.07 0 0 0.05
Nucleus 0.72 0.66 – 0.68 0.53 0.45 0.57 – 0.52 0.61 0.49 0.68 0.67 0.69 0.59 0.63 0.55 0.47 0.66 0.68 0.4 0.6 0.48 0.38
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0.1 0 0.06 – 0 0.05 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0
Vac/Vac Membrane 0.1 0.31 – 0.05 0.18 0.17 0.08 – 0.07 0.05 0.06 0 0.09 0 0.07 0.16 0.29 0.13 0.17 0.14 0.38 0.26 0.31 0.37
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 – 0 0 1 0 – 0 0 3 0 0 0 0 0 0 1 1 1 1 0 2 1
Bud Neck 0 0 – 1 3 1 1 – 0 3 0 2 0 0 2 0 0 0 0 0 0 0 1 1
Bud Site 0 0 – 0 0 1 4 – 1 2 5 4 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 14 16 – 30 23 49 50 – 17 27 13 16 9 45 51 7 10 25 7 4 2 2 13 22
Endoplasmic Reticulum 0 2 – 1 0 0 2 – 0 0 0 2 1 0 0 4 4 6 0 0 0 0 0 0
Endosome 0 1 – 1 8 7 5 – 0 4 2 0 1 1 2 3 4 7 3 4 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 1 2 0 2 0 2 0 0 0 0
Mitochondria 75 3 – 7 3 35 69 – 92 80 121 105 8 1 3 3 2 4 1 2 4 0 3 8
Nucleus 160 97 – 96 77 84 156 – 85 132 84 148 29 106 89 43 34 46 168 118 25 18 64 68
Nuclear Periphery 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Nucleolus 3 3 – 0 2 2 3 – 2 5 4 4 0 0 0 1 1 0 9 3 0 0 0 1
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 – 5 15 3 15 – 1 11 3 2 1 2 4 0 1 2 12 8 0 0 3 5
Vac/Vac Membrane 23 45 – 7 26 32 21 – 12 10 11 8 4 5 11 11 18 13 44 25 24 8 41 67
Unique Cell Count 223 147 141 145 185 272 163 215 170 217 43 154 151 68 62 97 256 174 64 31 135 183
Labelled Cell Count 277 168 148 157 215 328 210 274 246 291 53 160 163 74 75 108 256 174 64 31 135 183


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.9 – 5.1 5.7 4.6 4.6 – 3.9 4.3 3.8 3.8 5.2 5.4 5.5 7.9 7.2 7.1 – 5.1 5.3
Std Deviation (1e-4) 0.5 0.7 – 1.0 1.6 1.5 1.3 – 1.3 1.2 1.5 0.7 1.1 0.8 1.6 3.4 1.7 2.4 – 1.1 1.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –