Standard name
Human Ortholog
Description 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.97 0.95 0.88 0.87 0.79 0.77 0.71 0.71 0.63 0.99 0.99 0.98 0.93 0.9 0.92 0.96 0.9 0.94 0.93 0.97 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.05 0.16 0.14 0.18 0.31 0.36 0.34 0.46 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0.06 0 0 0 0 0.09 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 3 1 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2 2 0 3 2 7 7 5 8 6 9 1 0 7 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 13 1 4 4 4 10 11 20 13 21 18 27 0 3 7 6 8 6 0 0 0 0 0 0
Cytoplasm 268 199 268 218 200 323 376 439 234 329 260 323 186 248 258 186 131 200 257 187 269 158 259 194
Endoplasmic Reticulum 0 2 2 1 1 8 10 9 3 6 9 4 0 0 0 6 3 8 1 2 4 3 0 3
Endosome 1 0 1 0 0 0 0 2 0 0 0 0 0 0 0 2 1 1 2 3 0 1 1 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 1 1 1 0 0 0 0
Mitochondria 0 4 1 14 10 58 62 102 94 165 124 235 1 2 8 3 1 1 2 1 3 0 0 2
Nucleus 1 1 1 1 1 3 5 9 1 2 1 6 0 3 2 6 4 5 0 2 0 0 1 0
Nuclear Periphery 0 1 0 0 0 1 1 1 0 3 1 1 0 0 0 0 0 0 1 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 2 2 2 2 11 32 4 19 12 33 0 0 0 8 13 12 0 5 1 0 0 1
Unique Cell Count 272 204 273 224 211 367 434 558 305 462 364 512 187 251 262 200 146 218 269 209 285 171 268 209
Labelled Cell Count 284 211 281 240 221 408 485 624 355 553 431 642 188 257 284 219 162 234 269 209 285 171 268 209


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.9 8.9 6.7 6.5 7.0 6.7 6.4 6.5 6.1 5.8 5.8 5.5 9.3 9.6 8.3 9.8 10.8 11.0 9.3 10.0 10.6
Std Deviation (1e-4) 1.7 1.6 0.9 1.0 1.3 1.3 1.2 1.3 1.3 1.2 1.1 1.1 1.4 1.7 1.4 2.4 3.0 2.8 1.4 2.0 1.9
Intensity Change (Log2) – – – -0.06 0.06 -0.0 -0.06 -0.05 -0.14 -0.21 -0.21 -0.29 0.47 0.51 0.31 0.54 0.68 0.71 0.47 0.57 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.1 1.0 1.7 2.0 2.2 2.4 2.6 0 0 1.0 0 0 0
Cytoplasm -0.6 -2.1 -4.8 -5.2 -7.4 -7.6 -9.0 -8.9 -10.9 1.2 0.6 0.3 -2.8 -3.9 -3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.8 0 6.7 6.3 7.4 9.9 11.1 10.6 13.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.7 3.4 0 2.7 2.2 3.7 0 0 0 0 4.3 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1479 5.8953 5.7232 5.0409 4.4225 5.1601 4.7128 6.3515 5.1482 5.199 4.8324 5.1108 5.9482 6.5182 6.267 6.4588 6.3727 6.2594
Actin 0.0002 0.0003 0.0007 0.0004 0.0003 0.0004 0.0577 0.001 0.0165 0.0143 0.042 0.0096 0.0198 0.0046 0.0249 0.0136 0.0311 0.0036
Bud 0.0001 0.0004 0.0004 0.0002 0.0001 0.0002 0.0013 0.0007 0.0009 0.0008 0.0029 0.0002 0.004 0.0013 0.0087 0.0021 0.0023 0.0035
Bud Neck 0.0002 0.0002 0.0004 0.0005 0.0004 0.0012 0.0123 0.0002 0.0003 0.0016 0.0014 0.0006 0.0052 0.0002 0.0007 0.0017 0.0295 0.001
Bud Periphery 0.0001 0.0002 0.0003 0.0001 0.0001 0.0003 0.002 0.0004 0.0007 0.0009 0.0043 0.0002 0.0026 0.0006 0.0019 0.0008 0.0034 0.0013
Bud Site 0.0002 0.0024 0.0014 0.0004 0.0002 0.0002 0.0046 0.0039 0.0026 0.0013 0.0125 0.0001 0.0094 0.0064 0.0111 0.0012 0.0192 0.0005
Cell Periphery 0.0006 0.0008 0.0004 0.0002 0.0002 0.0002 0.0006 0.0002 0.0001 0.0001 0.0005 0 0.0005 0.0001 0.0003 0.0001 0.0002 0.0001
Cytoplasm 0.9174 0.9683 0.9458 0.9371 0.8955 0.9515 0.6298 0.9043 0.8791 0.8172 0.723 0.8975 0.753 0.9182 0.8344 0.8367 0.7298 0.922
Cytoplasmic Foci 0.0026 0.0036 0.004 0.0059 0.0055 0.0036 0.0421 0.0088 0.0205 0.0256 0.0312 0.0103 0.0274 0.0135 0.0109 0.0259 0.0256 0.0096
Eisosomes 0 0 0 0 0.0006 0 0.0019 0 0.0001 0.0001 0.0002 0 0.0002 0 0.0003 0.0001 0.0001 0
Endoplasmic Reticulum 0.019 0.0019 0.0035 0.0027 0.0017 0.0077 0.0092 0.0016 0.0024 0.004 0.0049 0.0029 0.0119 0.0016 0.0034 0.0021 0.0023 0.0011
Endosome 0.0026 0.0027 0.0047 0.0087 0.0094 0.0061 0.0575 0.0032 0.0195 0.038 0.0674 0.015 0.02 0.0027 0.0112 0.0064 0.0351 0.0023
Golgi 0.0002 0.0003 0.0004 0.0004 0.0004 0.0006 0.017 0.0012 0.0104 0.0211 0.0247 0.0041 0.006 0.0007 0.0025 0.004 0.0132 0.0004
Lipid Particles 0.0004 0.0001 0.0015 0.0002 0 0.0001 0.0227 0.0002 0.0045 0.0161 0.0107 0.0016 0.0049 0.0003 0.0018 0.03 0.0011 0
Mitochondria 0.0001 0.0002 0.0001 0.0002 0.0002 0.0024 0.0116 0.0003 0.002 0.0106 0.0263 0.0088 0.0026 0.0003 0.0116 0.0019 0.0139 0.0006
None 0.0521 0.0142 0.0256 0.0363 0.0823 0.0202 0.0842 0.0681 0.0302 0.0213 0.0103 0.0393 0.1154 0.0448 0.0641 0.0527 0.0852 0.0468
Nuclear Periphery 0.0012 0.0005 0.0007 0.0008 0.0003 0.0008 0.0073 0.0002 0.0006 0.0072 0.0052 0.0026 0.0017 0.0004 0.0011 0.0006 0.0008 0.0004
Nucleolus 0 0 0.0001 0.0001 0.0002 0 0.0011 0 0.0001 0.0006 0.0016 0.0001 0.001 0 0.0002 0.0001 0.0001 0.0004
Nucleus 0.0007 0.0014 0.0014 0.0015 0.0008 0.0013 0.0028 0.0007 0.0009 0.0029 0.0029 0.0011 0.0025 0.0008 0.0045 0.0013 0.0009 0.0045
Peroxisomes 0 0.0001 0.0001 0 0 0 0.0234 0.0025 0.0057 0.0064 0.0043 0.0033 0.007 0.0022 0.0028 0.0152 0.003 0.0005
Punctate Nuclear 0.0001 0.0001 0.0002 0.0004 0.0002 0.0001 0.0054 0.0002 0.0007 0.0058 0.0099 0.0017 0.0018 0.0003 0.0015 0.002 0.0007 0.0008
Vacuole 0.002 0.0022 0.0078 0.0035 0.0012 0.0025 0.0041 0.0021 0.0018 0.0027 0.0106 0.0008 0.0026 0.0007 0.0015 0.0012 0.0019 0.0005
Vacuole Periphery 0.0001 0.0002 0.0004 0.0003 0.0002 0.0005 0.0014 0.0002 0.0003 0.0013 0.0033 0.0004 0.0005 0.0001 0.0004 0.0003 0.0007 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.7194 18.1385 17.251 20.3474 21.3054 20.2793 20.3417 24.4076 25.6176 24.1274
Translational Efficiency 1.1368 1.0809 0.9258 0.9791 0.6984 1.0835 1.0278 0.678 0.6754 0.6871

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1213 1273 226 1155 119 2103 2438 1343 1332 3376 2664 2498

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 766.36 1037.37 1482.33 1079.53 1029.89 982.84 1196.85 1105.55 789.90 1003.40 1221.07 1093.52
Standard Deviation 107.19 149.00 196.44 167.00 134.14 142.56 152.58 172.65 133.12 147.41 175.80 170.55
Intensity Change Log 2 — 0.436836 0.951772 0.494309 — -0.067462 0.216752 0.102274 — 0.169517 0.576803 0.282698

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000135 0.000204 0.000603 0.000625 0.000701 0.000306 0.000309 0.000556 0.000185 0.000268 0.000334 0.000588
Bud Neck 0.002037 0.001210 0.002633 0.002644 0.001491 0.002298 0.001436 0.001363 0.001988 0.001888 0.001538 0.001955
Bud Site 0.007469 0.011544 0.010987 0.026184 0.023463 0.015627 0.005501 0.016294 0.008898 0.014088 0.005966 0.020867
Cell Periphery 0.000094 0.000120 0.000214 0.000123 0.000320 0.000058 0.000037 0.000069 0.000114 0.000081 0.000052 0.000094
Cytoplasm 0.762471* 0.804252* 0.565179* 0.628028* 0.637562* 0.762773* 0.788216* 0.814969* 0.751312* 0.778414* 0.769295* 0.728534*
Cytoplasmic Foci 0.095760 0.099914 0.048737 0.052014 0.191580* 0.125374 0.016531 0.022841 0.104321 0.115773 0.019263 0.036330
Eisosomes 0.000023 0.000011 0.000030 0.000024 0.000050 0.000017 0.000009 0.000010 0.000026 0.000015 0.000011 0.000017
Endoplasmic Reticulum 0.001009 0.000949 0.001872 0.000674 0.003174 0.000636 0.000564 0.000332 0.001203 0.000754 0.000675 0.000490
Endosome 0.006246 0.007430 0.013268 0.020638 0.027530 0.009072 0.001689 0.003289 0.008148 0.008453 0.002671 0.011311
Golgi 0.001700 0.003020 0.000582 0.015735 0.006423 0.005719 0.000352 0.002669 0.002122 0.004701 0.000372 0.008710
Lipid Particles 0.001963 0.000795 0.003042 0.005871 0.002967 0.000680 0.000463 0.000563 0.002053 0.000723 0.000682 0.003017
Mitochondria 0.001555 0.001152 0.000072 0.009963 0.003562 0.002019 0.000155 0.001182 0.001734 0.001692 0.000148 0.005242
Mitotic Spindle 0.000639 0.003848 0.009322 0.028712 0.009243 0.004992 0.002821 0.022028 0.001407 0.004561 0.003373 0.025118
None 0.008973 0.002176 0.000775 0.003361 0.000495 0.001772 0.002384 0.002485 0.008216 0.001924 0.002247 0.002890
Nuclear Periphery 0.000141 0.000154 0.000677 0.000304 0.000492 0.000054 0.000349 0.000127 0.000172 0.000092 0.000377 0.000209
Nuclear Periphery Foci 0.001238 0.001321 0.012991 0.002170 0.005681 0.000503 0.001289 0.001820 0.001635 0.000812 0.002282 0.001982
Nucleolus 0.000558 0.000265 0.000137 0.000228 0.000889 0.000230 0.000129 0.000095 0.000587 0.000243 0.000129 0.000156
Nucleus 0.041991 0.016930 0.041183 0.034022 0.013586 0.012168 0.080604 0.045673 0.039454 0.013963 0.077260 0.040286
Peroxisomes 0.000666 0.000877 0.000357 0.004642 0.000980 0.001910 0.000243 0.001132 0.000694 0.001520 0.000253 0.002755
Vacuole 0.063992 0.040458 0.286716* 0.158420 0.059871 0.051924 0.096718 0.059886 0.063624 0.047600 0.112836 0.105445
Vacuole Periphery 0.001339 0.003370 0.000622 0.005619 0.009939 0.001868 0.000202 0.002618 0.002107 0.002434 0.000238 0.004006

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.00 -8.13 -5.19 -3.90 4.06 2.81 3.12 2.06 -1.26 -1.85 -1.82 -0.86 -4.70 -2.71 -1.71
Bud Neck 2.32 -3.77 0.57 -2.78 5.62 -2.91 0.09 1.16 6.08 1.14 0.26 1.03 1.68 2.92 0.67
Bud Site -3.40 -4.56 -3.27 -0.67 0.34 1.99 5.00 3.52 3.97 -4.75 -4.59 3.82 -1.90 2.62 -6.14
Cell Periphery -1.39 -9.18 1.17 1.81 9.20 5.77 6.25 5.41 -4.17 -11.63 2.99 9.05 4.83 0.52 -5.28
Cytoplasm -4.64 14.37 18.94 22.93 -3.66 -5.34 -3.90 -3.61 4.77 0.64 -3.69 5.54 12.49 19.22 8.43
Cytoplasmic Foci -0.72 11.02 16.63 16.56 3.79 4.75 12.95 12.48 29.58 -5.36 -2.56 23.72 21.36 33.01 -6.18
Eisosomes 8.78 -3.68 1.91 -8.73 5.26 3.75 4.61 4.33 2.43 -2.93 6.24 9.15 6.37 -1.27 -5.56
Endoplasmic Reticulum 0.22 -3.74 3.82 1.79 7.85 3.55 3.90 4.20 1.49 3.87 2.15 4.24 6.00 2.14 4.51
Endosome -1.21 -3.76 0.41 1.20 3.22 3.32 4.71 4.71 11.03 0.14 -0.21 8.60 5.59 7.14 -2.40
Golgi -2.04 3.95 -1.85 -0.61 -2.94 0.29 2.11 1.91 7.33 -1.65 -4.52 4.89 -0.03 3.74 -3.55
Lipid Particles 2.80 -3.32 -0.37 -1.91 1.53 3.76 4.02 3.36 -3.36 -6.36 3.59 3.46 0.98 -2.30 -2.17
Mitochondria 0.55 2.42 -0.80 -1.27 -2.56 0.96 2.25 2.09 2.57 -2.24 0.07 2.83 0.57 0.59 -2.79
Mitotic Spindle -2.86 -3.06 -6.80 -4.75 -2.18 0.60 0.86 -0.08 -2.22 -3.29 -3.00 -2.25 -6.96 -4.76 -5.47
None 4.44 5.57 4.52 0.13 -4.79 -4.46 -9.88 -8.19 -1.63 1.10 4.59 4.41 4.44 -0.97 0.33
Nuclear Periphery -0.20 -11.20 -12.53 -5.55 5.47 1.13 0.07 0.56 -11.40 5.31 1.72 -6.79 -5.67 -10.19 2.94
Nuclear Periphery Foci -0.79 -12.24 -5.33 -3.76 11.37 4.58 3.72 3.19 -11.89 -3.94 5.48 -4.52 -2.66 -11.69 2.67
Nucleolus 2.48 5.24 4.96 1.64 -0.57 0.89 1.01 1.08 3.62 6.17 3.21 4.50 4.73 3.36 2.17
Nucleus 9.87 -2.77 -3.67 -15.05 0.28 0.58 -27.22 -15.35 -23.32 11.77 12.13 -19.35 -7.26 -27.95 14.68
Peroxisomes -0.84 2.34 -1.36 -0.82 -2.25 -2.69 3.75 2.09 4.73 -2.20 -3.61 3.75 -0.55 2.54 -3.19
Vacuole 5.99 -16.30 -27.16 -30.79 2.67 0.92 -10.09 -11.05 -18.93 -2.55 4.89 -19.46 -28.01 -34.38 -10.17
Vacuole Periphery -2.48 4.16 -0.08 2.17 -2.02 2.20 2.68 2.50 1.92 -1.11 -0.62 5.09 2.10 2.85 -2.44
Standard name
Human Ortholog
Description 5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Oxp1

Oxp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oxp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available