Standard name | |
---|---|
Human Ortholog | |
Description | Ubiquitin-specific protease involved in transport and osmotic response; negatively regulates Ras/PKA signaling; interacts with Bre5p to coregulate anterograde, retrograde transport between ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; role in ribophagy; cleaves ubiquitin fusions but not polyubiquitin; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 1 | 2 | 10 | 12 | 14 | 7 | 11 | 17 | 18 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 3 | 1 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 4 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 19 | 22 | 17 | 14 | 24 | 15 | 18 | 10 | 12 | 17 | 14 | 6 | 8 | 11 | 17 | 31 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 296 | 498 | 439 | 218 | 278 | 269 | 247 | 230 | 153 | 159 | 151 | 136 | 271 | 290 | 327 | 369 | 314 | 342 | 277 | 501 | 438 | 147 | 245 | 155 |
Endoplasmic Reticulum | 8 | 26 | 37 | 27 | 33 | 59 | 60 | 79 | 42 | 46 | 39 | 38 | 5 | 13 | 15 | 13 | 12 | 12 | 9 | 7 | 25 | 35 | 97 | 73 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 6 |
Golgi | 0 | 0 | 1 | 1 | 1 | 3 | 5 | 8 | 2 | 1 | 0 | 4 | 0 | 0 | 1 | 8 | 2 | 1 | 0 | 0 | 3 | 2 | 12 | 10 |
Mitochondria | 0 | 1 | 0 | 1 | 2 | 15 | 42 | 15 | 37 | 63 | 87 | 57 | 2 | 1 | 0 | 5 | 0 | 2 | 0 | 2 | 6 | 3 | 9 | 4 |
Nucleus | 0 | 0 | 2 | 0 | 1 | 0 | 3 | 5 | 5 | 3 | 2 | 3 | 2 | 1 | 2 | 1 | 5 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 2 | 2 | 3 | 1 | 6 | 5 | 13 | 16 | 11 | 15 | 8 | 25 | 0 | 0 | 1 | 48 | 13 | 17 | 2 | 9 | 15 | 8 | 17 | 21 |
Unique Cell Count | 305 | 522 | 476 | 250 | 318 | 346 | 353 | 351 | 231 | 272 | 273 | 246 | 280 | 305 | 343 | 420 | 342 | 369 | 300 | 531 | 497 | 207 | 401 | 285 |
Labelled Cell Count | 310 | 547 | 506 | 266 | 338 | 385 | 400 | 389 | 270 | 311 | 324 | 297 | 286 | 313 | 358 | 464 | 380 | 391 | 300 | 531 | 497 | 207 | 401 | 285 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.8 | 17.1 | 13.5 | 12.5 | 13.5 | 11.8 | 11.7 | 12.0 | 11.0 | 10.2 | 9.2 | 10.0 | 15.3 | 16.3 | 16.4 | 16.4 | 18.0 | 17.1 | 14.1 | 15.1 | 16.0 |
Std Deviation (1e-4) | 3.0 | 2.8 | 1.8 | 1.7 | 1.6 | 1.9 | 1.9 | 1.8 | 2.0 | 1.8 | 1.9 | 1.9 | 2.4 | 2.3 | 2.6 | 4.0 | 3.7 | 3.8 | 2.8 | 2.5 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.0 | -0.19 | -0.2 | -0.17 | -0.29 | -0.4 | -0.55 | -0.43 | 0.19 | 0.27 | 0.29 | 0.28 | 0.42 | 0.35 | 0.07 | 0.17 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.7 | 4.0 | 0 | 0 | 5.2 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.2 | -0.1 | 1.4 | -0.3 | 0.3 | -0.2 | -0.1 | 1.0 | 0.6 | -1.7 | -1.4 | -1.0 | -0.4 | 2.5 | -1.0 |
Cytoplasm | -2.2 | -2.2 | -5.9 | -8.4 | -9.7 | -8.8 | -11.1 | -11.9 | -11.7 | 2.5 | 1.6 | 1.8 | -2.2 | -0.2 | 0.2 |
Endoplasmic Reticulum | 1.4 | 1.3 | 4.1 | 4.1 | 6.0 | 4.1 | 3.8 | 2.8 | 3.2 | -3.5 | -2.0 | -2.0 | -3.0 | -2.5 | -2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.6 | 7.7 | 4.6 | 9.0 | 11.0 | 13.1 | 10.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.2 | 3.7 | 0 | 4.2 | 0 | 6.3 | 0 | 0 | 0 | 7.0 | 3.2 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.1607 | 15.734 | 13.4232 | 11.5687 | 11.1686 | 13.6283 | 11.7596 | 16.1865 | 14.7565 | 12.2784 | 12.6342 | 14.516 | 10.8645 | 14.3946 | 13.2516 | 13.2177 | 11.5316 | 13.0438 |
Actin | 0.0094 | 0.0023 | 0.0055 | 0.0235 | 0.0199 | 0.0073 | 0.0148 | 0.0006 | 0.0016 | 0.0229 | 0.0032 | 0.0014 | 0.0193 | 0.0043 | 0.0104 | 0.0323 | 0.0298 | 0.0055 |
Bud | 0.001 | 0.0006 | 0.0014 | 0.0015 | 0.0014 | 0.001 | 0.0025 | 0.0033 | 0.0014 | 0.0029 | 0.0006 | 0.001 | 0.0098 | 0.0119 | 0.0145 | 0.0117 | 0.0072 | 0.005 |
Bud Neck | 0.0036 | 0.0006 | 0.001 | 0.0014 | 0.0015 | 0.0035 | 0.0018 | 0.0004 | 0.0007 | 0.0014 | 0.0006 | 0.0011 | 0.0011 | 0.0007 | 0.0011 | 0.0012 | 0.001 | 0.002 |
Bud Periphery | 0.0012 | 0.0004 | 0.0013 | 0.0012 | 0.0018 | 0.001 | 0.0022 | 0.0008 | 0.0006 | 0.0036 | 0.0004 | 0.001 | 0.0057 | 0.0038 | 0.006 | 0.0069 | 0.0028 | 0.0018 |
Bud Site | 0.0042 | 0.0016 | 0.0043 | 0.0021 | 0.0139 | 0.001 | 0.0062 | 0.0068 | 0.0011 | 0.0078 | 0.0006 | 0.0002 | 0.0093 | 0.0415 | 0.0158 | 0.0082 | 0.0014 | 0.0033 |
Cell Periphery | 0.002 | 0.0012 | 0.0013 | 0.0006 | 0.0005 | 0.0005 | 0.0012 | 0.0003 | 0.0004 | 0.0013 | 0.0001 | 0.0003 | 0.0015 | 0.001 | 0.0009 | 0.0007 | 0.0004 | 0.0004 |
Cytoplasm | 0.6016 | 0.918 | 0.7614 | 0.6415 | 0.549 | 0.7196 | 0.7055 | 0.9028 | 0.863 | 0.6056 | 0.6994 | 0.7974 | 0.7169 | 0.8475 | 0.8207 | 0.7941 | 0.6896 | 0.8001 |
Cytoplasmic Foci | 0.023 | 0.0096 | 0.0215 | 0.0255 | 0.0341 | 0.033 | 0.0183 | 0.0135 | 0.0198 | 0.0238 | 0.0357 | 0.0218 | 0.0305 | 0.0299 | 0.0221 | 0.0359 | 0.0362 | 0.0305 |
Eisosomes | 0.0003 | 0 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0004 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.1374 | 0.0226 | 0.0343 | 0.0416 | 0.0606 | 0.078 | 0.0771 | 0.0155 | 0.0248 | 0.0271 | 0.0119 | 0.0444 | 0.0352 | 0.0108 | 0.0163 | 0.0061 | 0.007 | 0.0346 |
Endosome | 0.1034 | 0.0242 | 0.078 | 0.0848 | 0.1462 | 0.0847 | 0.0821 | 0.0339 | 0.0508 | 0.1564 | 0.1682 | 0.0825 | 0.0714 | 0.0247 | 0.0468 | 0.0475 | 0.1492 | 0.0752 |
Golgi | 0.0188 | 0.0076 | 0.0116 | 0.0389 | 0.0277 | 0.0185 | 0.0173 | 0.006 | 0.0085 | 0.0256 | 0.0205 | 0.0189 | 0.0105 | 0.0031 | 0.0069 | 0.0097 | 0.0116 | 0.01 |
Lipid Particles | 0.0088 | 0.0002 | 0.0071 | 0.0078 | 0.03 | 0.0006 | 0.004 | 0.0001 | 0.0003 | 0.0048 | 0.004 | 0.0001 | 0.0046 | 0.0009 | 0.0007 | 0.0147 | 0.0024 | 0.0008 |
Mitochondria | 0.0172 | 0.0024 | 0.0126 | 0.0414 | 0.0324 | 0.0269 | 0.0274 | 0.0026 | 0.0041 | 0.0542 | 0.0133 | 0.0177 | 0.0188 | 0.002 | 0.005 | 0.0016 | 0.0021 | 0.0086 |
None | 0.0036 | 0.0005 | 0.0026 | 0.0006 | 0.0007 | 0.0011 | 0.0019 | 0.0005 | 0.0008 | 0.0015 | 0.002 | 0.0007 | 0.0109 | 0.0035 | 0.003 | 0.0064 | 0.0066 | 0.0022 |
Nuclear Periphery | 0.0116 | 0.0011 | 0.004 | 0.0044 | 0.007 | 0.0035 | 0.0069 | 0.0008 | 0.0013 | 0.0032 | 0.0152 | 0.001 | 0.0076 | 0.001 | 0.0022 | 0.0017 | 0.003 | 0.0041 |
Nucleolus | 0.001 | 0 | 0.0008 | 0.0001 | 0.0004 | 0.0009 | 0.0003 | 0 | 0 | 0.0003 | 0.0003 | 0 | 0.0038 | 0.0005 | 0.0028 | 0.0002 | 0.0003 | 0.0003 |
Nucleus | 0.0059 | 0.0009 | 0.0075 | 0.0015 | 0.0026 | 0.0021 | 0.0028 | 0.0008 | 0.0009 | 0.0019 | 0.0026 | 0.0007 | 0.0079 | 0.0031 | 0.0041 | 0.0017 | 0.0033 | 0.0029 |
Peroxisomes | 0.0018 | 0.0001 | 0.0037 | 0.0286 | 0.0384 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0.0064 | 0.0009 | 0.0001 | 0.0013 | 0.0005 | 0.0005 | 0.0092 | 0.0029 | 0.0004 |
Punctate Nuclear | 0.0056 | 0.0001 | 0.0005 | 0.0003 | 0.0011 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0016 | 0.0007 | 0 | 0.0038 | 0.0009 | 0.0008 | 0.0027 | 0.0026 | 0.0028 |
Vacuole | 0.0319 | 0.0047 | 0.0318 | 0.0417 | 0.019 | 0.0114 | 0.0195 | 0.0091 | 0.016 | 0.0331 | 0.0124 | 0.006 | 0.0253 | 0.007 | 0.0164 | 0.0056 | 0.0332 | 0.0069 |
Vacuole Periphery | 0.0066 | 0.0012 | 0.0072 | 0.0109 | 0.0112 | 0.0045 | 0.0059 | 0.0019 | 0.0037 | 0.0145 | 0.0073 | 0.0037 | 0.0045 | 0.0014 | 0.003 | 0.0017 | 0.0073 | 0.0026 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.4864 | 33.8515 | 25.6538 | 42.8157 | 43.8742 | 30.5996 | 49.1562 | 51.9775 | 38.1641 | 50.9308 |
Translational Efficiency | 1.3224 | 1.6973 | 1.6755 | 0.8687 | 1.0396 | 1.4217 | 1.1567 | 0.8779 | 1.0431 | 0.9098 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1744 | 1738 | 474 | 1938 | 2381 | 2187 | 592 | 1129 | 4125 | 3925 | 1066 | 3067 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1267.36 | 1495.68 | 1853.05 | 1587.81 | 1334.52 | 1377.30 | 1597.42 | 1478.10 | 1306.13 | 1429.72 | 1711.09 | 1547.42 |
Standard Deviation | 186.04 | 241.15 | 241.96 | 211.76 | 212.27 | 206.83 | 204.03 | 248.49 | 204.31 | 230.31 | 255.51 | 232.09 |
Intensity Change Log 2 | — | 0.238975 | 0.548075 | 0.325212 | — | 0.045522 | 0.259423 | 0.147423 | — | 0.142994 | 0.407238 | 0.236761 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000219 | 0.000183 | 0.000366 | 0.000254 | 0.000091 | 0.000108 | 0.000428 | 0.000449 | 0.000145 | 0.000141 | 0.000400 | 0.000326 |
Bud Neck | 0.000158 | 0.000220 | 0.003168 | 0.000901 | 0.000120 | 0.000150 | 0.002026 | 0.001162 | 0.000136 | 0.000181 | 0.002534 | 0.000997 |
Bud Site | 0.021109 | 0.016186 | 0.021168 | 0.020373 | 0.013299 | 0.013243 | 0.024843 | 0.042052 | 0.016601 | 0.014546 | 0.023209 | 0.028353 |
Cell Periphery | 0.000565 | 0.000569 | 0.001679 | 0.001954 | 0.000590 | 0.000361 | 0.001025 | 0.001364 | 0.000579 | 0.000453 | 0.001315 | 0.001737 |
Cytoplasm | 0.677910* | 0.760681* | 0.647127* | 0.797180* | 0.730529* | 0.742739* | 0.732085* | 0.694988* | 0.708282* | 0.750684* | 0.694308* | 0.759562* |
Cytoplasmic Foci | 0.197320* | 0.122394 | 0.047997 | 0.033270 | 0.143116 | 0.149014 | 0.062726 | 0.080244 | 0.166033 | 0.137226 | 0.056176 | 0.050562 |
Eisosomes | 0.000003 | 0.000003 | 0.000003 | 0.000003 | 0.000002 | 0.000002 | 0.000004 | 0.000004 | 0.000003 | 0.000003 | 0.000004 | 0.000004 |
Endoplasmic Reticulum | 0.009229 | 0.006962 | 0.002732 | 0.001163 | 0.006860 | 0.003940 | 0.004907 | 0.001916 | 0.007861 | 0.005278 | 0.003940 | 0.001440 |
Endosome | 0.005499 | 0.005439 | 0.014358 | 0.004992 | 0.004247 | 0.006303 | 0.014456 | 0.011548 | 0.004776 | 0.005921 | 0.014413 | 0.007405 |
Golgi | 0.032728 | 0.037431 | 0.044701 | 0.019795 | 0.029185 | 0.035667 | 0.025520 | 0.033976 | 0.030683 | 0.036448 | 0.034049 | 0.025015 |
Lipid Particles | 0.000654 | 0.001111 | 0.005566 | 0.006596 | 0.000779 | 0.000673 | 0.007489 | 0.006997 | 0.000726 | 0.000867 | 0.006634 | 0.006744 |
Mitochondria | 0.000811 | 0.000495 | 0.000089 | 0.000264 | 0.000963 | 0.000879 | 0.000042 | 0.000185 | 0.000899 | 0.000709 | 0.000063 | 0.000235 |
Mitotic Spindle | 0.001386 | 0.003025 | 0.025112 | 0.001589 | 0.000971 | 0.001721 | 0.005578 | 0.002815 | 0.001147 | 0.002298 | 0.014264 | 0.002040 |
None | 0.000286 | 0.000528 | 0.000359 | 0.000111 | 0.001052 | 0.000250 | 0.000245 | 0.000107 | 0.000728 | 0.000373 | 0.000296 | 0.000109 |
Nuclear Periphery | 0.000025 | 0.000042 | 0.001030 | 0.000024 | 0.000030 | 0.000032 | 0.000145 | 0.000030 | 0.000028 | 0.000036 | 0.000538 | 0.000026 |
Nuclear Periphery Foci | 0.000792 | 0.001618 | 0.016857 | 0.001443 | 0.000756 | 0.000781 | 0.007333 | 0.001940 | 0.000772 | 0.001151 | 0.011568 | 0.001626 |
Nucleolus | 0.000017 | 0.000025 | 0.000089 | 0.000108 | 0.000022 | 0.000025 | 0.000053 | 0.000061 | 0.000020 | 0.000025 | 0.000069 | 0.000091 |
Nucleus | 0.000648 | 0.000758 | 0.004329 | 0.001912 | 0.001091 | 0.001047 | 0.002968 | 0.001571 | 0.000903 | 0.000919 | 0.003573 | 0.001787 |
Peroxisomes | 0.000120 | 0.000118 | 0.000144 | 0.000459 | 0.000108 | 0.000128 | 0.000249 | 0.000700 | 0.000113 | 0.000124 | 0.000202 | 0.000548 |
Vacuole | 0.010119 | 0.013236 | 0.108356 | 0.088398 | 0.011936 | 0.015019 | 0.092967 | 0.094294 | 0.011168 | 0.014230 | 0.099810 | 0.090569 |
Vacuole Periphery | 0.040403 | 0.028975 | 0.054770 | 0.019210 | 0.054254 | 0.027916 | 0.014911 | 0.023595 | 0.048398 | 0.028385 | 0.032635 | 0.020824 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.59 | -1.78 | -0.74 | -2.69 | 1.62 | -1.40 | -8.04 | -7.66 | -7.19 | -0.29 | 0.14 | -5.85 | -5.95 | -8.06 | 1.75 |
Bud Neck | -2.80 | -7.75 | -11.24 | -10.16 | 5.84 | -2.30 | -8.91 | -12.53 | -12.19 | 3.66 | -3.68 | -11.30 | -16.63 | -15.72 | 7.10 |
Bud Site | 4.02 | -0.22 | 0.32 | -3.57 | 0.39 | 0.05 | -5.32 | -10.65 | -10.78 | -5.02 | 2.81 | -4.13 | -9.27 | -11.20 | -2.51 |
Cell Periphery | -0.09 | -4.27 | -5.07 | -5.03 | -0.68 | 2.41 | -3.47 | -4.14 | -5.11 | -1.53 | 2.18 | -5.42 | -6.17 | -6.73 | -1.79 |
Cytoplasm | -8.67 | 2.41 | -13.12 | -4.06 | -10.26 | -1.51 | 0.24 | 3.92 | 5.11 | 2.49 | -6.87 | 1.96 | -7.65 | -0.99 | -6.73 |
Cytoplasmic Foci | 11.71 | 25.90 | 31.09 | 21.39 | 5.00 | -1.12 | 16.66 | 13.61 | 14.17 | -3.48 | 7.06 | 29.58 | 34.50 | 27.62 | 2.53 |
Eisosomes | 1.08 | 0.31 | -0.17 | -1.23 | -0.45 | -0.03 | -5.78 | -6.29 | -5.95 | 0.31 | 0.81 | -4.19 | -4.76 | -4.47 | 0.66 |
Endoplasmic Reticulum | 1.83 | 6.10 | 9.75 | 6.18 | 2.20 | 4.23 | 1.50 | 8.24 | 4.18 | 2.41 | 3.87 | 4.51 | 13.26 | 7.85 | 3.30 |
Endosome | 0.12 | -7.15 | 1.11 | 0.89 | 7.40 | -5.71 | -8.94 | -11.13 | -7.44 | 2.45 | -4.23 | -11.48 | -7.41 | -3.75 | 8.04 |
Golgi | -2.58 | -3.49 | 8.38 | 9.88 | 7.64 | -4.12 | 1.85 | -1.78 | 1.32 | -2.85 | -4.84 | -1.47 | 5.25 | 8.86 | 4.53 |
Lipid Particles | -4.33 | -7.12 | -12.47 | -11.34 | -1.02 | 1.23 | -9.10 | -8.50 | -8.65 | 0.52 | -2.10 | -11.54 | -14.88 | -14.49 | -0.01 |
Mitochondria | 2.46 | 6.41 | 4.52 | 2.95 | -3.36 | 0.32 | 13.98 | 9.60 | 2.80 | -2.86 | 1.25 | 13.70 | 9.49 | 3.34 | -4.61 |
Mitotic Spindle | -2.32 | -7.08 | -0.64 | 1.68 | 6.95 | -3.52 | -5.27 | -3.10 | -2.02 | 2.34 | -3.44 | -8.26 | -2.79 | 0.11 | 7.43 |
None | -1.21 | -1.98 | 7.95 | 2.08 | 7.03 | 2.41 | 2.42 | 2.84 | 6.05 | 2.93 | 1.68 | 2.22 | 3.22 | 2.95 | 6.33 |
Nuclear Periphery | -1.75 | -3.02 | -0.14 | 1.77 | 3.02 | -0.56 | -3.93 | -1.11 | -0.19 | 3.83 | -1.78 | -3.44 | -0.28 | 1.70 | 3.44 |
Nuclear Periphery Foci | -3.78 | -8.81 | -5.49 | 0.70 | 8.42 | -0.24 | -7.78 | -8.29 | -7.63 | 6.28 | -3.35 | -11.41 | -9.52 | -3.81 | 10.44 |
Nucleolus | -1.15 | -5.29 | -16.60 | -10.54 | -0.62 | -1.11 | -5.08 | -10.42 | -9.15 | -1.34 | -1.46 | -6.99 | -20.43 | -15.17 | -2.21 |
Nucleus | -2.22 | -4.23 | -10.75 | -9.84 | 2.86 | 0.17 | -6.13 | -3.14 | -3.83 | 4.17 | -0.16 | -6.16 | -7.57 | -8.52 | 4.04 |
Peroxisomes | 0.10 | -1.52 | -10.76 | -10.89 | -9.06 | -1.68 | -6.08 | -8.18 | -7.92 | -5.90 | -1.15 | -5.88 | -13.14 | -12.90 | -9.59 |
Vacuole | -2.40 | -12.94 | -24.30 | -23.07 | 2.61 | -2.94 | -12.17 | -19.05 | -18.26 | -0.44 | -3.74 | -17.70 | -30.90 | -29.45 | 1.64 |
Vacuole Periphery | 4.31 | -2.74 | 9.60 | 4.98 | 7.79 | 9.91 | 13.23 | 11.36 | 2.52 | -3.32 | 10.49 | 5.95 | 15.62 | 5.87 | 4.78 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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