Standard name
Human Ortholog
Description Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0.08 0.1 0.08 0.08 0.09 0.15 0.1 0.1 0.09 0.1 0.12 0.19 0.21 0.1 0.08 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.2 0.09 0.29 0.24 0.37 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.75 0.48 0.71 0.81 0.78 0.74 0.67 0.62 0.57 0.62 0.53 0.61 0.77 0.7 0.66 0.6 0.54 0.49 0.86 0.79 0.81 0.7 0.64 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.24 0.53 0.26 0.11 0.14 0.19 0.15 0.21 0.17 0.15 0.18 0.24 0.13 0.14 0.14 0.34 0.38 0.37 0.05 0.12 0.07 0.12 0.14 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 0 1 2 1 2 0 2 4 0 0 0 0 0 0 3 3 4 8 13 19
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 0 1 2 4 2 5 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 1 0 0 0 0 0 0 0 0
Cytoplasm 4 8 16 22 18 21 29 35 27 27 32 33 31 77 90 29 16 17 1 2 9 6 13 12
Endoplasmic Reticulum 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 9 3 6 0 1 1 0 1 0
Endosome 0 0 3 2 3 0 1 2 3 0 0 3 2 4 2 2 0 2 2 0 2 2 6 14
Golgi 0 2 0 0 1 0 0 0 0 0 0 0 0 1 0 18 16 3 0 0 0 0 1 0
Mitochondria 2 0 0 0 0 18 63 21 78 63 127 42 0 1 2 2 3 6 0 0 2 1 1 2
Nucleus 76 55 146 178 177 204 207 148 154 162 182 201 193 288 282 179 116 81 117 103 190 171 252 275
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 1 1 1 0 1 6 2 6 2 8 3 0 1 0 3 0 2 2 1 1 13 32 29
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 3 4 11 6
Vac/Vac Membrane 24 60 54 25 32 54 47 50 46 39 63 80 34 58 61 101 82 61 6 15 17 28 56 80
Unique Cell Count 102 114 206 219 227 277 311 240 271 260 342 330 252 412 425 300 213 164 137 131 235 244 397 455
Labelled Cell Count 106 126 221 228 232 299 356 261 320 296 420 373 260 432 437 344 237 178 137 131 235 244 397 455


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 15.4 11.0 10.9 10.3 8.5 7.6 7.9 7.3 7.3 6.9 7.4 12.9 12.8 12.2 18.1 18.0 18.1 12.8 12.0 12.8
Std Deviation (1e-4) 1.6 2.6 2.7 1.9 2.1 2.0 1.7 1.6 1.8 1.6 1.7 1.5 3.4 3.1 2.8 4.0 3.8 4.4 2.8 2.4 2.6
Intensity Change (Log2) – – – -0.01 -0.1 -0.37 -0.52 -0.48 -0.59 -0.6 -0.68 -0.57 0.23 0.22 0.15 0.72 0.71 0.72 0.22 0.12 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.1 -0.1 0.6 2.3 0.8 1.0 0.6 0.9 1.6 3.6 4.2 0.7 -0.1 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.6 4.0 0
Mitochondria 0 0 3.7 6.9 4.3 8.4 7.6 10.0 5.3 0 0 0 0 0 0
Nucleus 2.5 1.7 0.7 -1.0 -2.0 -3.1 -1.9 -4.1 -2.3 1.4 -0.2 -1.1 -2.6 -3.5 -4.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.9 -3.2 -1.8 -3.1 -1.3 -2.5 -3.0 -2.2 -0.5 -3.4 -3.7 -3.6 1.8 2.7 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.0196 11.3221 9.9759 8.444 9.1735 9.9294 9.8503 11.9867 10.9728 9.6662 9.3267 10.9973 11.3544 12.4613 11.9598 10.41 10.128 12.0911
Actin 0.0073 0 0 0.0017 0.0076 0 0.0449 0 0.0086 0.0004 0.0751 0.0024 0.0162 0 0.0046 0.0005 0.0411 0.0098
Bud 0.0002 0 0 0.0004 0.0001 0 0.0022 0 0.0003 0.0001 0.0002 0.0001 0.0016 0 0.0002 0.0004 0.0002 0.0001
Bud Neck 0.0012 0.0001 0.0001 0.0004 0.0008 0.0006 0.0083 0 0.0004 0.0009 0.0003 0.0023 0.0008 0 0.0002 0.0005 0.0016 0.001
Bud Periphery 0.0004 0 0.0001 0.0014 0.0002 0 0.0034 0 0.0003 0.0003 0.0003 0.0002 0.0037 0 0.0003 0.0004 0.0003 0.0002
Bud Site 0.0008 0 0.0001 0.0008 0.0006 0 0.0071 0 0.0051 0.0008 0.0006 0.0002 0.0031 0 0.0009 0.0004 0.0005 0.0001
Cell Periphery 0.0002 0 0.0001 0.0008 0.0001 0 0.0012 0 0.0002 0.0006 0 0 0.0003 0 0.0001 0.0005 0.0001 0
Cytoplasm 0.0015 0.0003 0.0002 0.0022 0.004 0 0.0104 0.0002 0.0032 0.0568 0.0001 0.0011 0.0037 0.0001 0.0003 0.0111 0.0053 0.0002
Cytoplasmic Foci 0.0015 0.0018 0 0.0013 0.0015 0 0.0281 0 0.0085 0.0046 0.0105 0.0032 0.0066 0 0.0028 0.0118 0.0368 0.001
Eisosomes 0.0001 0 0 0.0001 0.0001 0 0.0003 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0013 0 0.0001 0.0015 0.0027 0 0.0056 0 0.002 0.0036 0 0.0002 0.0039 0 0.0003 0.0014 0.0069 0.0001
Endosome 0.0032 0.0003 0.0001 0.003 0.0171 0 0.0241 0 0.0058 0.0349 0.0009 0.0019 0.0124 0 0.0018 0.0029 0.0453 0.0012
Golgi 0.001 0.0001 0 0.001 0.0017 0 0.0082 0 0.0015 0.0027 0.0058 0.0027 0.0042 0 0.002 0.0006 0.0136 0.0012
Lipid Particles 0.0019 0.0005 0 0.0089 0.0013 0 0.0098 0 0.0038 0.0271 0.0068 0.007 0.009 0 0.0011 0.0263 0.0369 0.0009
Mitochondria 0.0013 0.0001 0.0003 0.0339 0.0028 0 0.0109 0.0001 0.0018 0.0107 0.0018 0.001 0.0032 0.0001 0.004 0.0067 0.002 0.0071
None 0.0016 0.0064 0 0.0008 0.0039 0 0.0048 0 0.0015 0.001 0.0002 0.0001 0.002 0 0.0002 0.0014 0.0081 0.0001
Nuclear Periphery 0.0029 0.0022 0.0015 0.0092 0.0183 0.0003 0.0188 0.0011 0.0024 0.0156 0.0005 0.0017 0.0117 0.001 0.0029 0.0057 0.0311 0.0009
Nucleolus 0.006 0.004 0.0057 0.008 0.0275 0.013 0.0135 0.0029 0.0039 0.01 0.0104 0.0077 0.0045 0.0033 0.0023 0.0058 0.0176 0.0046
Nucleus 0.9606 0.9806 0.9891 0.9115 0.898 0.9812 0.7617 0.9956 0.9458 0.815 0.8199 0.9506 0.8986 0.9953 0.9718 0.912 0.6951 0.9694
Peroxisomes 0.0027 0.0007 0 0.0007 0.0003 0 0.0121 0 0.0015 0.0035 0.0639 0.0078 0.0035 0 0.0024 0.0009 0.0202 0.0009
Punctate Nuclear 0.0019 0.0023 0.0018 0.0035 0.0066 0.0047 0.0159 0.0001 0.001 0.0012 0.0023 0.0093 0.006 0.0001 0.0012 0.0011 0.0327 0.0008
Vacuole 0.0017 0.0005 0.0005 0.0052 0.0026 0.0001 0.0053 0 0.0018 0.0072 0.0001 0.0003 0.0037 0 0.0003 0.0048 0.0022 0.0003
Vacuole Periphery 0.0006 0.0001 0.0003 0.0036 0.0021 0 0.0035 0 0.0003 0.0031 0.0001 0.0001 0.001 0 0.0003 0.0047 0.002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 122.058 89.2297 96.4396 76.2247 96.3588 83.4957 68.4005 47.324 58.7338 65.8727
Translational Efficiency 1.6804 1.828 1.5231 1.9516 1.8948 1.7439 1.4424 2.0545 1.571 1.759

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
823 376 2546 1290 478 2004 431 155 1301 2380 2977 1445

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 971.63 1267.49 1534.97 1594.96 1112.26 1230.68 1638.97 1464.04 1023.30 1236.50 1550.03 1580.92
Standard Deviation 148.51 222.48 229.87 331.36 165.11 191.33 222.12 262.45 169.01 197.04 231.67 327.19
Intensity Change Log 2 — 0.383495 0.659732 0.715041 — 0.145962 0.559295 0.396461 — 0.261593 0.606996 0.553781

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000571 0.000790 0.000217 0.000523 0.000603 0.000393 0.000820 0.000546 0.000583 0.000456 0.000304 0.000526
Bud Neck 0.004065 0.014351 0.011642 0.015636 0.005788 0.012437 0.010553 0.009086 0.004698 0.012739 0.011485 0.014934
Bud Site 0.001295 0.002361 0.000750 0.002671 0.000819 0.002524 0.001022 0.003200 0.001120 0.002498 0.000790 0.002727
Cell Periphery 0.000230 0.000151 0.000063 0.000131 0.000092 0.000145 0.000102 0.000137 0.000179 0.000146 0.000068 0.000132
Cytoplasm 0.026710 0.003899 0.004327 0.008680 0.003633 0.013520 0.002385 0.064423 0.018231 0.012000 0.004046 0.014659
Cytoplasmic Foci 0.001195 0.000140 0.000020 0.000120 0.000131 0.001473 0.000040 0.011522 0.000804 0.001262 0.000023 0.001343
Eisosomes 0.000021 0.000023 0.000013 0.000018 0.000021 0.000019 0.000042 0.000016 0.000021 0.000020 0.000017 0.000017
Endoplasmic Reticulum 0.006944 0.003947 0.000883 0.000992 0.007285 0.004291 0.002180 0.001770 0.007069 0.004236 0.001071 0.001076
Endosome 0.000424 0.000421 0.000044 0.000150 0.000197 0.001231 0.000066 0.003809 0.000340 0.001103 0.000047 0.000543
Golgi 0.000316 0.000098 0.000024 0.000045 0.000057 0.000923 0.000048 0.000130 0.000221 0.000793 0.000027 0.000054
Lipid Particles 0.000395 0.000106 0.000012 0.000050 0.000194 0.000373 0.000050 0.000557 0.000321 0.000331 0.000017 0.000104
Mitochondria 0.001005 0.001034 0.000921 0.001651 0.001723 0.001342 0.001319 0.001319 0.001269 0.001293 0.000979 0.001615
Mitotic Spindle 0.002589 0.001914 0.000364 0.001987 0.002397 0.006125 0.000021 0.083906 0.002519 0.005459 0.000314 0.010774
None 0.004563 0.001648 0.001490 0.003281 0.001989 0.002797 0.001393 0.017482 0.003618 0.002615 0.001476 0.004804
Nuclear Periphery 0.000445 0.000264 0.000433 0.000793 0.000354 0.000713 0.000269 0.001389 0.000412 0.000642 0.000409 0.000857
Nuclear Periphery Foci 0.000455 0.000479 0.000010 0.000606 0.000136 0.000371 0.000015 0.003847 0.000338 0.000388 0.000011 0.000954
Nucleolus 0.001567 0.001283 0.000217 0.000374 0.001408 0.001607 0.000732 0.001136 0.001508 0.001555 0.000292 0.000455
Nucleus 0.944387* 0.965670* 0.978013* 0.961212* 0.971461* 0.946050* 0.977075* 0.789430* 0.954334* 0.949150* 0.977877* 0.942786*
Peroxisomes 0.000415 0.000142 0.000014 0.000034 0.000183 0.000504 0.000120 0.000131 0.000330 0.000447 0.000029 0.000044
Vacuole 0.002136 0.001190 0.000499 0.000994 0.001426 0.002686 0.001699 0.005994 0.001876 0.002450 0.000673 0.001530
Vacuole Periphery 0.000270 0.000090 0.000043 0.000053 0.000102 0.000478 0.000048 0.000170 0.000209 0.000417 0.000044 0.000065

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.09 4.44 -0.80 0.55 -5.06 4.17 -5.26 -5.09 -6.24 -2.95 2.22 4.55 -1.95 -3.71 -5.27
Bud Neck -5.26 -9.99 -10.13 -1.77 -3.72 -6.89 -7.38 -4.37 -2.68 -2.61 -9.88 -10.98 -10.84 -4.56 -4.37
Bud Site -1.46 2.18 -2.32 -0.65 -3.42 -3.87 -3.97 -4.15 -3.03 -3.81 -3.14 1.37 -3.94 -1.38 -4.46
Cell Periphery 1.36 4.21 2.16 0.25 -9.11 -3.02 -0.65 -5.20 -2.78 -4.27 1.18 4.31 1.06 -0.37 -9.70
Cytoplasm 6.84 6.85 5.59 -4.65 -5.54 -6.87 1.72 -3.77 -1.99 -4.09 1.95 6.76 3.47 2.17 -6.72
Cytoplasmic Foci 2.22 2.45 2.25 0.15 -2.99 -3.08 1.29 -1.09 -0.26 -1.14 -0.83 2.54 1.45 2.32 -1.61
Eisosomes -0.68 4.68 -1.54 -0.37 -9.29 1.33 -8.83 -5.76 -6.32 -0.47 1.32 1.73 -3.88 -5.97 -6.56
Endoplasmic Reticulum 3.28 15.40 14.44 2.95 -3.50 6.62 11.89 4.22 0.03 -3.44 7.81 20.65 18.37 11.43 -4.17
Endosome 0.12 7.04 4.95 0.97 -2.62 -3.14 6.03 -1.78 0.14 -2.00 -2.64 8.27 1.62 3.01 -2.91
Golgi 1.21 1.64 1.43 0.58 -7.79 -2.02 -0.79 -6.12 1.49 -6.49 -1.45 1.67 1.21 1.94 -9.41
Lipid Particles 2.64 3.77 3.11 0.39 -3.53 -0.96 1.13 -2.69 -1.21 -4.75 0.06 4.00 2.47 1.44 -5.42
Mitochondria -0.12 -0.25 -2.72 -4.49 -10.54 0.27 0.06 -0.87 -4.59 -4.48 -0.16 0.17 -1.81 -4.77 -11.16
Mitotic Spindle 1.01 2.16 0.72 -0.49 -2.41 -1.61 1.03 -3.61 -3.36 -3.79 -1.09 2.31 -2.16 -1.38 -4.38
None 3.04 3.34 1.21 -4.71 -6.54 -1.25 1.03 -3.80 -3.49 -4.11 1.32 3.43 -1.02 -3.16 -7.36
Nuclear Periphery 3.43 -0.90 -4.35 -6.04 -4.13 -4.83 -0.10 -6.35 -4.91 -6.38 -4.19 -1.50 -6.49 -3.13 -6.08
Nuclear Periphery Foci -0.01 4.77 0.45 0.26 -1.38 -2.35 3.57 -1.23 -1.13 -1.28 -0.56 5.39 -0.92 -0.71 -1.85
Nucleolus -0.02 6.80 5.15 1.78 -4.32 -0.87 3.45 -2.06 -1.31 -3.14 -0.40 8.51 4.82 2.92 -5.00
Nucleus -3.33 -6.65 -2.14 1.90 7.05 6.26 -0.27 7.11 5.77 7.21 1.46 -6.35 2.76 1.52 9.60
Peroxisomes 1.19 1.69 1.56 2.22 -2.69 -1.06 0.65 -0.20 1.03 -1.74 -0.28 1.93 1.74 1.55 -2.69
Vacuole 3.55 6.94 0.36 -2.68 -4.87 -4.14 -3.98 -4.26 -3.98 -4.07 -2.06 6.28 -3.88 -2.70 -5.35
Vacuole Periphery 1.40 1.74 1.69 1.39 -0.53 -2.64 1.15 -1.80 2.09 -5.21 -1.39 1.93 1.70 3.02 -1.48
Standard name
Human Ortholog
Description Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1
Localization
Cell Percentages nucleus (83%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Myg1

Myg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Myg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available