Standard name
Human Ortholog
Description Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.09 0.11 0 0.08 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.06 0 0.1 0.07 0.12 0.17 – 0.26 0.23 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.84 0.89 0.83 0.89 0.9 0.85 0.8 0.72 0.73 – 0.67 0.69 0.89 0.84 0.79 0.88 0.73 0.76 0.66 0.82 0.81 0.78 0.71 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.51 0.3 0.34 0.19 0.17 0.3 0.33 0.39 0.41 – 0.4 0.48 0.28 0.24 0.31 0.17 0.19 0.17 0.3 0.09 0.14 0.14 0.21 0.27
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 2 1 – 3 3 1 0 0 1 0 0 0 5 0 0 5 1
Bud Neck 0 0 0 0 0 0 0 3 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 2 8 7 7 8 – 5 15 1 2 3 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 5 9 10 9 15 17 8 – 10 8 13 37 44 3 11 14 0 5 1 1 0 0
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 4 4 0 0 0 0 0 0
Endosome 0 1 0 0 0 0 0 0 0 – 0 0 0 1 2 0 2 1 1 2 2 1 0 2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 2
Mitochondria 1 8 30 17 9 48 43 80 76 – 105 126 0 5 8 3 1 9 2 0 1 1 0 2
Nucleus 204 288 197 262 389 419 462 475 319 – 274 373 231 342 327 106 105 138 171 299 199 159 238 235
Nuclear Periphery 1 2 0 0 2 3 17 6 6 – 8 14 1 0 1 2 0 1 0 0 0 0 0 0
Nucleolus 124 97 82 55 74 150 188 257 179 – 165 261 74 97 130 20 27 30 79 31 34 27 71 102
Peroxisomes 0 0 0 0 0 1 0 0 0 – 0 0 1 1 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 3 1 – 2 2 2 5 5 1 1 1 1 1 0 1 2 1
Vac/Vac Membrane 1 3 0 0 0 1 0 4 1 – 2 2 2 1 1 1 3 6 2 14 5 5 16 33
Unique Cell Count 244 322 238 293 430 495 578 657 437 411 543 260 408 416 121 143 181 262 363 247 204 337 386
Labelled Cell Count 334 406 314 345 486 640 734 854 599 574 805 326 491 526 137 155 204 262 363 247 204 337 386


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.0 10.7 7.1 7.5 7.6 7.0 6.8 6.5 6.0 – 5.4 5.4 11.3 10.7 8.9 9.8 11.4 11.6 10.7 10.3 11.8
Std Deviation (1e-4) 3.9 2.5 1.7 2.3 1.7 2.4 1.8 2.0 1.8 – 1.7 1.5 2.9 3.7 3.2 3.1 3.1 3.3 2.8 3.6 2.6
Intensity Change (Log2) – – – 0.09 0.11 -0.02 -0.07 -0.12 -0.25 – -0.4 -0.39 0.68 0.6 0.34 0.47 0.69 0.71 0.59 0.54 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.2 0 0.4 0.4 0 – 0.3 0 1.7 3.5 4.0 0 2.6 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria -2.7 -5.5 -1.2 -2.3 -0.2 1.6 – 3.9 3.4 -5.9 -6.2 -5.6 -3.1 -4.1 -2.7
Nucleus 2.2 2.9 0.6 -0.9 -3.2 -2.9 – -4.4 -4.1 1.9 0.3 -1.3 1.2 -2.2 -1.7
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus -4.1 -5.0 -1.1 -0.5 1.3 1.7 – 1.4 3.5 -1.4 -2.9 -0.8 -3.6 -3.3 -4.1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6767 9.0059 9.0382 8.2841 7.4579 8.4906 7.9708 9.3115 8.5684 8.3254 8.0528 8.3582 6.2058 7.2169 6.1343 5.6918 5.3517 5.6183
Actin 0.0355 0 0.0001 0 0.0078 0.0001 0.0015 0.0004 0.0037 0.0003 0 0.0001 0.0005 0 0.0125 0.001 0 0
Bud 0.0006 0 0.0005 0 0.0109 0 0.0005 0.0001 0.0006 0.0001 0 0 0.0003 0 0.0001 0.0004 0.0001 0
Bud Neck 0.0038 0 0.0003 0 0.0003 0.0005 0.0004 0.0001 0 0.0001 0.0001 0.0002 0.0004 0 0.0002 0.0002 0.0004 0.0003
Bud Periphery 0.0011 0 0.0011 0 0.0433 0.0001 0.0009 0.0001 0.0011 0.0001 0 0 0.001 0 0.0002 0.0003 0.0003 0
Bud Site 0.0085 0 0.0007 0 0.0003 0.0003 0.0022 0.0001 0.0011 0.0002 0 0 0.0006 0 0.0005 0.009 0.0001 0
Cell Periphery 0.0006 0 0.0005 0 0.0002 0 0.0002 0 0 0 0 0 0.0007 0 0.0001 0.0001 0.0003 0
Cytoplasm 0.0015 0 0.0001 0 0.0002 0.0001 0.0041 0.0004 0.0001 0.0006 0 0.0043 0.0053 0 0.0001 0.0016 0 0
Cytoplasmic Foci 0.0121 0 0.0001 0 0 0.0045 0.0131 0 0.0002 0.0004 0 0 0.0065 0 0.0004 0.0099 0 0.0006
Eisosomes 0.0007 0 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.003 0 0 0 0 0 0.0013 0.0003 0.0003 0.0002 0 0.0003 0.0011 0 0.0001 0.0006 0 0.0001
Endosome 0.0112 0 0 0 0 0.0007 0.0083 0.0002 0.0008 0.0009 0 0.0002 0.0046 0 0.0002 0.0107 0 0.0002
Golgi 0.0049 0 0 0 0 0.0003 0.0026 0 0.0004 0.0002 0 0 0.0004 0 0.0005 0.0023 0 0.0001
Lipid Particles 0.0156 0 0.0001 0 0 0.0022 0.0104 0 0.0002 0.0003 0 0 0.0092 0 0.0002 0.0057 0.0001 0.0057
Mitochondria 0.0073 0.0001 0.0009 0 0.0006 0.0001 0.0049 0.0003 0.0168 0.0019 0.0005 0.0002 0.0199 0.0001 0.0005 0.0014 0.0039 0.0001
None 0.0022 0 0.0001 0 0 0.0001 0.0019 0 0.0003 0.0001 0 0.0001 0.0137 0 0.0001 0.0011 0 0
Nuclear Periphery 0.0083 0.0001 0.0002 0.0001 0.0002 0.0002 0.0134 0.0045 0.0006 0.0201 0.0002 0.0037 0.0085 0.0002 0.0026 0.0006 0.0003 0.0035
Nucleolus 0.0747 0.0475 0.0674 0.0374 0.2319 0.1806 0.0698 0.0286 0.0316 0.0322 0.2261 0.159 0.0877 0.044 0.0443 0.0135 0.2552 0.1616
Nucleus 0.7882 0.952 0.9264 0.9622 0.7037 0.8035 0.8252 0.9639 0.9409 0.9131 0.7724 0.8309 0.8239 0.9553 0.9358 0.9358 0.7337 0.8256
Peroxisomes 0.0084 0 0.0001 0 0 0.0006 0.0043 0 0.0007 0.0001 0 0 0.0003 0 0.0002 0.0018 0.0001 0
Punctate Nuclear 0.0086 0.0001 0.0003 0.0001 0.0003 0.0061 0.0292 0.0005 0.0002 0.0271 0.0002 0.0006 0.0082 0.0001 0.0009 0.0019 0.0005 0.0016
Vacuole 0.0019 0 0.0009 0 0.0001 0.0001 0.0029 0.0002 0.0001 0.0006 0.0001 0.0001 0.0031 0.0001 0.0004 0.0016 0.0014 0.0001
Vacuole Periphery 0.0012 0 0.0002 0 0.0001 0 0.0026 0.0002 0.0002 0.0014 0.0002 0.0001 0.004 0 0.0002 0.0003 0.0034 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.7932 19.6962 18.4354 19.0074 23.6975 23.0218 26.9868 27.9515 25.9917 26.5026
Translational Efficiency 0.7978 1.0141 0.8003 0.8592 0.8589 1.1266 0.902 0.6899 0.5875 0.6477

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2258 836 1055 1126 1713 2049 258 1332 3971 2885 1313 2458

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 799.42 1114.67 1498.63 1243.99 972.18 1033.15 1378.79 1286.92 873.94 1056.77 1475.08 1267.25
Standard Deviation 141.46 219.35 252.25 218.34 191.03 184.49 186.14 235.60 185.58 198.70 245.36 228.86
Intensity Change Log 2 — 0.479591 0.906619 0.637949 — 0.087754 0.504107 0.404627 — 0.277820 0.699723 0.514603

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000386 0.001088 0.000876 0.001479 0.000423 0.000607 0.000795 0.000793 0.000402 0.000746 0.000860 0.001107
Bud Neck 0.006012 0.007913 0.020367 0.025796 0.005806 0.009459 0.011495 0.020209 0.005923 0.009011 0.018624 0.022769
Bud Site 0.000599 0.001580 0.001541 0.004816 0.000920 0.001269 0.000939 0.003133 0.000737 0.001359 0.001422 0.003904
Cell Periphery 0.000171 0.000078 0.000041 0.000190 0.000056 0.000101 0.000029 0.000300 0.000122 0.000094 0.000038 0.000250
Cytoplasm 0.000449 0.001236 0.000131 0.001410 0.000069 0.000238 0.000064 0.001750 0.000285 0.000527 0.000118 0.001594
Cytoplasmic Foci 0.000056 0.001283 0.000099 0.004241 0.000045 0.000337 0.000033 0.000563 0.000052 0.000611 0.000086 0.002248
Eisosomes 0.000068 0.000082 0.000116 0.000063 0.000086 0.000110 0.000090 0.000066 0.000076 0.000102 0.000111 0.000065
Endoplasmic Reticulum 0.002151 0.001494 0.000743 0.001818 0.001488 0.001965 0.000814 0.001078 0.001865 0.001829 0.000757 0.001417
Endosome 0.000257 0.000527 0.000036 0.003879 0.000082 0.000420 0.000022 0.001708 0.000182 0.000451 0.000033 0.002703
Golgi 0.000074 0.001228 0.000101 0.004784 0.000097 0.000389 0.000064 0.000330 0.000084 0.000632 0.000094 0.002371
Lipid Particles 0.000034 0.001020 0.000020 0.000943 0.000025 0.000390 0.000015 0.000538 0.000030 0.000572 0.000019 0.000724
Mitochondria 0.000690 0.002103 0.002053 0.006661 0.000719 0.002425 0.001348 0.002440 0.000703 0.002331 0.001915 0.004374
Mitotic Spindle 0.000655 0.005270 0.000551 0.016449 0.000277 0.004550 0.000069 0.004766 0.000492 0.004758 0.000456 0.010118
None 0.003767 0.001160 0.000718 0.002338 0.001277 0.000721 0.001194 0.002295 0.002693 0.000848 0.000812 0.002315
Nuclear Periphery 0.000282 0.000275 0.000085 0.002229 0.000059 0.000212 0.000044 0.000402 0.000186 0.000230 0.000077 0.001239
Nuclear Periphery Foci 0.000323 0.000490 0.000033 0.001067 0.000259 0.000489 0.000005 0.000440 0.000295 0.000489 0.000028 0.000727
Nucleolus 0.094963 0.103045 0.080996 0.034654 0.082011 0.105230 0.052146 0.036489 0.089376 0.104597 0.075327 0.035648
Nucleus 0.887542* 0.865935* 0.886309* 0.878910* 0.904684* 0.867477* 0.927817* 0.919008* 0.894937* 0.867031* 0.894465* 0.900639*
Peroxisomes 0.000478 0.002893 0.004529 0.001580 0.001024 0.001268 0.002576 0.001073 0.000714 0.001739 0.004146 0.001305
Vacuole 0.000563 0.000789 0.000409 0.003530 0.000256 0.001042 0.000310 0.001864 0.000431 0.000969 0.000390 0.002627
Vacuole Periphery 0.000479 0.000512 0.000247 0.003162 0.000337 0.001302 0.000127 0.000755 0.000418 0.001073 0.000224 0.001857

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.01 -18.51 -6.13 -1.24 -2.78 -3.56 -8.26 -11.13 -5.16 -1.05 -3.21 -19.90 -8.95 -3.45 -2.41
Bud Neck -2.71 -16.91 -14.99 -12.43 -4.18 -5.44 -5.21 -13.94 -10.19 -6.26 -6.12 -17.75 -20.43 -15.78 -4.82
Bud Site -1.91 -9.54 -5.49 -3.10 -3.75 -0.88 -0.47 -3.21 -2.94 -3.33 -2.38 -5.21 -6.06 -4.61 -4.42
Cell Periphery 1.60 2.40 0.05 -2.56 -4.85 -1.83 1.73 -1.32 -1.01 -1.50 0.77 2.58 -1.07 -1.43 -2.09
Cytoplasm -0.96 2.49 -2.53 -0.24 -3.35 -2.19 0.15 -2.36 -2.12 -2.36 -1.00 2.11 -3.10 -2.24 -3.45
Cytoplasmic Foci -2.37 -2.44 -3.25 -1.07 -3.17 -2.53 0.45 -2.35 -0.63 -2.42 -3.19 -2.72 -3.75 -1.81 -3.61
Eisosomes -2.36 -9.12 0.36 3.66 13.32 -3.53 -1.09 3.86 7.26 4.26 -5.11 -9.11 2.45 8.18 14.24
Endoplasmic Reticulum 3.18 9.21 0.19 -2.99 -8.81 -2.53 5.14 1.58 3.37 -2.39 0.10 9.90 1.91 1.24 -7.05
Endosome -1.47 3.77 -3.76 -3.09 -4.13 -3.75 2.18 -2.44 -1.76 -2.55 -3.12 4.06 -4.30 -3.50 -4.66
Golgi -1.81 -5.15 -2.64 -1.38 -2.59 -2.15 0.77 -2.29 0.47 -2.65 -2.64 -2.39 -2.86 -1.65 -2.79
Lipid Particles -1.75 0.17 -1.93 0.62 -1.93 -2.09 0.79 -1.56 -0.17 -1.58 -2.64 0.15 -2.46 0.17 -2.46
Mitochondria -2.46 -14.12 -4.73 -2.99 -3.17 -2.82 -5.60 -8.06 -0.35 -5.03 -3.54 -15.71 -6.65 -2.53 -3.73
Mitotic Spindle -2.54 -0.10 -5.42 -3.00 -5.41 -3.69 1.31 -4.25 -1.43 -4.39 -4.35 -0.35 -6.84 -3.43 -6.79
None 3.93 4.83 2.13 -2.86 -4.77 1.80 0.24 -1.75 -2.94 -1.71 4.83 4.72 0.65 -4.30 -4.19
Nuclear Periphery -0.08 2.63 -3.30 -3.05 -3.71 -3.01 -0.16 -5.52 -3.17 -5.51 -0.70 2.19 -4.24 -3.88 -4.69
Nuclear Periphery Foci -0.96 3.25 -1.43 -0.48 -2.50 -1.14 2.34 -1.00 0.27 -3.35 -1.41 3.77 -1.72 -0.31 -3.69
Nucleolus -1.78 1.92 14.85 12.96 10.21 -5.76 5.05 11.15 17.14 1.86 -5.04 2.66 18.77 21.67 10.72
Nucleus 3.65 2.15 1.32 -1.78 -0.23 7.95 -3.37 -0.37 -6.91 2.74 7.70 2.05 0.26 -5.75 -1.42
Peroxisomes -8.34 -13.10 -6.23 3.96 9.06 -1.53 -4.49 -0.92 0.81 4.28 -8.75 -13.11 -5.93 2.83 10.70
Vacuole -1.16 -0.33 -3.87 -2.85 -3.83 -2.93 -2.18 -2.95 -1.63 -2.76 -2.63 -1.21 -4.62 -3.04 -4.32
Vacuole Periphery -0.24 2.33 -2.15 -2.08 -2.46 -2.74 3.60 -1.41 1.48 -2.29 -2.56 3.08 -2.46 -0.53 -3.00
Standard name
Human Ortholog
Description Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Chd1

Chd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Chd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available