Standard name
Human Ortholog
Description 5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.92 1.0 0.95 0.97 0.84 0.82 – 0.66 0.74 0.56 0.58 0.98 0.98 1.0 0.94 0.94 0.94 0.68 0.7 0.71 0.64 0.64 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.08 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0 0 0.24 0.1 – 0.36 0.36 0.46 0.55 0 0 0 0.07 0 0 0.21 0.14 0.18 0.21 0.2 0.17
Nucleus 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.06 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 1 3 1 5 8 13
Bud 2 2 0 1 0 0 1 – 4 1 0 3 0 2 0 0 0 0 1 2 0 2 8 10
Bud Neck 0 4 0 1 4 0 3 – 1 1 0 0 0 0 0 2 0 0 1 1 0 3 3 4
Bud Site 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 4 1 0 2 – 0 2 0 0 2 3 2 0 2 1 1 0 0 3 1 4
Cytoplasm 259 185 24 167 210 107 199 – 57 92 33 75 227 428 466 127 90 105 202 151 53 188 256 259
Endoplasmic Reticulum 0 1 0 0 0 0 0 – 1 0 1 0 0 0 0 9 8 11 4 1 1 3 1 3
Endosome 4 0 0 0 4 1 6 – 0 1 1 0 0 2 1 2 1 1 7 4 0 8 8 4
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 0 0 1 1 0 0 1 1
Mitochondria 9 16 0 5 1 31 24 – 31 45 27 71 1 1 0 10 4 3 63 30 13 60 81 67
Nucleus 0 1 0 1 1 3 4 – 5 3 2 3 0 1 0 0 1 0 0 1 0 0 1 2
Nuclear Periphery 0 1 0 0 0 1 2 – 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 0
Nucleolus 0 1 0 0 0 1 0 – 3 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 1 – 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 2 0 5 1 0 15 – 1 4 3 4 0 0 1 0 0 0 4 3 0 5 10 7
Vac/Vac Membrane 8 1 0 2 6 2 12 – 3 1 1 1 2 1 0 2 1 1 3 3 0 3 8 6
Unique Cell Count 272 202 24 176 217 127 242 86 125 59 130 232 435 468 135 96 112 299 215 75 292 403 395
Labelled Cell Count 283 216 24 187 228 146 269 106 150 70 158 234 441 471 153 108 122 299 215 75 292 403 395


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.9 5.4 6.2 5.1 3.8 4.5 – 4.3 4.3 4.5 3.8 4.8 4.9 4.9 4.9 5.0 5.1 4.1 4.4 4.6
Std Deviation (1e-4) 0.5 1.1 3.2 6.7 1.4 1.7 2.0 – 2.3 2.4 2.4 1.8 1.7 1.6 1.7 0.8 0.7 1.2 1.3 1.9 2.0
Intensity Change (Log2) – – – 0.2 -0.07 -0.51 -0.26 – -0.32 -0.32 -0.25 -0.49 -0.16 -0.13 -0.12 -0.13 -0.1 -0.08 -0.38 -0.28 -0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.9355 -1.507 -1.6744 -1.7195 -1.6156 -1.4682 0.0297 -0.2872 -0.0563 -0.7479 -0.552 -0.3469 1.04 1.5005 1.4734 1.8171 1.274 1.5698
Actin 0.0056 0.0004 0.0022 0.0008 0.0279 0.0008 0.0311 0.0111 0.0181 0.0011 0.0019 0.0022 0.0244 0.0075 0.0136 0.0133 0.0228 0.0054
Bud 0.002 0.0009 0.0007 0.0001 0.0006 0.0013 0.001 0.0012 0.001 0.0003 0.0003 0.0013 0.0011 0.0001 0.0024 0.001 0.0011 0.0004
Bud Neck 0.0005 0.0002 0.0012 0.0006 0.0006 0.0007 0.0026 0.0008 0.0023 0.0006 0.0007 0.0028 0.0003 0.0001 0.0008 0.0005 0.0017 0.002
Bud Periphery 0.0009 0.0008 0.0008 0.0002 0.0012 0.0005 0.0016 0.0016 0.002 0.0003 0.0014 0.0033 0.0032 0.0001 0.0036 0.0019 0.0031 0.0009
Bud Site 0.0014 0.012 0.0071 0.0002 0.0011 0.0017 0.01 0.0188 0.0073 0.0007 0.0006 0.0006 0.001 0.0011 0.0032 0.0039 0.0031 0.0021
Cell Periphery 0.0003 0.0005 0.0002 0.0001 0.0001 0.0001 0.0005 0.0004 0.0004 0.0001 0.0005 0.0002 0.0004 0.0001 0.0004 0.0003 0.0004 0.0002
Cytoplasm 0.0861 0.0995 0.083 0.0533 0.1555 0.1307 0.1954 0.3138 0.2688 0.4199 0.3501 0.319 0.0903 0.2018 0.1256 0.1341 0.1173 0.2336
Cytoplasmic Foci 0.0419 0.04 0.0312 0.0425 0.0327 0.039 0.0521 0.0402 0.0391 0.0309 0.0501 0.0334 0.0299 0.0201 0.0247 0.036 0.0562 0.0277
Eisosomes 0.0008 0.0001 0.0005 0.0004 0.0002 0.0002 0.0007 0.0003 0.0004 0.0001 0.0003 0.0001 0.0005 0.0001 0.0004 0.0004 0.0007 0.0001
Endoplasmic Reticulum 0.003 0.0022 0.0015 0.0007 0.0017 0.0011 0.0112 0.0028 0.0044 0.0031 0.0023 0.005 0.0025 0.0005 0.0022 0.0023 0.0015 0.0013
Endosome 0.0142 0.0211 0.0062 0.0226 0.0267 0.0127 0.058 0.0161 0.0269 0.0186 0.0418 0.0188 0.0134 0.0025 0.0123 0.0201 0.0126 0.0082
Golgi 0.0017 0.0016 0.0006 0.0005 0.003 0.0005 0.0092 0.0034 0.01 0.0032 0.0039 0.0049 0.0038 0.0016 0.0048 0.0054 0.0028 0.0032
Lipid Particles 0.0096 0.0037 0.0021 0.0015 0.0092 0.0023 0.0274 0.0024 0.0118 0.0004 0.0088 0.0061 0.0073 0.0025 0.0023 0.0018 0.0053 0.0037
Mitochondria 0.0022 0.0035 0.0016 0.0013 0.0193 0.0018 0.017 0.0048 0.0178 0.0015 0.009 0.0077 0.0062 0.0005 0.0032 0.0071 0.0059 0.0071
None 0.8043 0.8059 0.8456 0.8673 0.691 0.793 0.5133 0.5722 0.5673 0.5125 0.4987 0.5677 0.794 0.7497 0.7807 0.7643 0.7433 0.6951
Nuclear Periphery 0.0021 0.001 0.0013 0.0015 0.0087 0.0017 0.0213 0.0009 0.0022 0.0007 0.009 0.0064 0.0041 0.0002 0.0011 0.0009 0.0009 0.0009
Nucleolus 0.0013 0.0004 0.0007 0.0003 0.001 0.0005 0.002 0.0004 0.0006 0.0004 0.0037 0.0008 0.0004 0.0005 0.0003 0.0003 0.0005 0.0004
Nucleus 0.0027 0.0011 0.0027 0.0007 0.0022 0.0027 0.007 0.0017 0.0016 0.0013 0.0061 0.0078 0.0029 0.0007 0.0011 0.0008 0.0009 0.0008
Peroxisomes 0.0084 0.001 0.0023 0.0013 0.0057 0.0014 0.0154 0.002 0.0109 0.0005 0.0022 0.0045 0.0098 0.0079 0.0083 0.0033 0.0153 0.0034
Punctate Nuclear 0.0089 0.0013 0.0066 0.0013 0.0043 0.0057 0.0131 0.0017 0.0026 0.0015 0.0018 0.0039 0.0018 0.0012 0.0019 0.0008 0.0032 0.0023
Vacuole 0.0019 0.0022 0.0016 0.002 0.0051 0.0014 0.007 0.003 0.0032 0.0018 0.0037 0.0028 0.0021 0.001 0.0062 0.0011 0.001 0.0011
Vacuole Periphery 0.0004 0.0005 0.0003 0.001 0.0022 0.0003 0.0031 0.0005 0.0013 0.0005 0.0032 0.0008 0.0006 0.0001 0.0008 0.0004 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.5539 15.5091 12.6592 21.7434 19.1198 13.8716 19.2095 21.1675 20.1649 27.5787
Translational Efficiency 1.1343 1.2373 1.3276 0.9482 0.9724 1.9356 1.433 1.088 1.187 0.895

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
189 721 502 1639 2257 1689 271 1560 2446 2410 773 3199

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 641.08 731.01 939.47 719.37 665.17 782.99 851.12 807.81 663.31 767.44 908.50 762.50
Standard Deviation 89.94 126.05 102.01 98.12 80.04 103.71 99.93 129.27 81.10 113.39 109.71 122.62
Intensity Change Log 2 — 0.189387 0.551343 0.166230 — 0.235271 0.355639 0.280293 — 0.212934 0.455004 0.225439

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000332 0.000823 0.000415 0.000183 0.000197 0.000775 0.000577 0.000583 0.000207 0.000790 0.000472 0.000378
Bud Neck 0.049189 0.050326 0.003222 0.012283 0.023842 0.059226 0.002118 0.008929 0.025800 0.056563 0.002835 0.010647
Bud Site 0.022850 0.033471 0.005501 0.015071 0.007366 0.026225 0.003069 0.030549 0.008563 0.028393 0.004648 0.022619
Cell Periphery 0.000746 0.000178 0.000157 0.000655 0.000169 0.000165 0.000230 0.000184 0.000213 0.000169 0.000183 0.000425
Cytoplasm 0.262253* 0.302089* 0.289279* 0.452756* 0.433063* 0.366438* 0.427604* 0.540513* 0.419865* 0.347187* 0.337774* 0.495551*
Cytoplasmic Foci 0.310485* 0.259938* 0.011494 0.051320 0.219178* 0.203215* 0.024654 0.037611 0.226234* 0.220185* 0.016108 0.044635
Eisosomes 0.000712 0.000146 0.000061 0.000172 0.000151 0.000158 0.000078 0.000048 0.000195 0.000154 0.000067 0.000112
Endoplasmic Reticulum 0.001189 0.002020 0.006625 0.002523 0.001012 0.000805 0.007240 0.001805 0.001026 0.001168 0.006840 0.002173
Endosome 0.019228 0.039241 0.003856 0.008752 0.009181 0.019358 0.004586 0.006522 0.009957 0.025306 0.004112 0.007665
Golgi 0.011979 0.010194 0.000092 0.001530 0.002449 0.004700 0.000125 0.001536 0.003185 0.006344 0.000104 0.001533
Lipid Particles 0.027604 0.010827 0.002417 0.004017 0.011832 0.005364 0.002867 0.001645 0.013051 0.006999 0.002575 0.002860
Mitochondria 0.064801 0.023164 0.000469 0.002748 0.008750 0.014142 0.000328 0.003889 0.013081 0.016841 0.000420 0.003304
Mitotic Spindle 0.004777 0.011698 0.001463 0.009761 0.003311 0.009966 0.001097 0.023658 0.003424 0.010484 0.001335 0.016537
None 0.001587 0.003883 0.004526 0.017057 0.002856 0.002183 0.003657 0.005594 0.002758 0.002692 0.004221 0.011467
Nuclear Periphery 0.000548 0.000899 0.002722 0.001052 0.000656 0.000759 0.001470 0.001169 0.000648 0.000801 0.002283 0.001109
Nuclear Periphery Foci 0.000318 0.000786 0.002426 0.000653 0.000338 0.000292 0.005078 0.001089 0.000337 0.000440 0.003356 0.000866
Nucleolus 0.001342 0.003013 0.000605 0.001688 0.001392 0.001617 0.000578 0.000782 0.001388 0.002035 0.000595 0.001247
Nucleus 0.144262* 0.101395 0.571434* 0.309063* 0.227100* 0.168644* 0.387057* 0.232536* 0.220700* 0.148525* 0.506795* 0.271744*
Peroxisomes 0.042878 0.029056 0.000291 0.002928 0.009314 0.023995 0.000420 0.001950 0.011907 0.025509 0.000336 0.002451
Vacuole 0.029345 0.111600 0.092755 0.104115 0.036945 0.089621 0.126942 0.097627 0.036358 0.096196 0.104741 0.100951
Vacuole Periphery 0.003576 0.005253 0.000187 0.001673 0.000896 0.002351 0.000226 0.001782 0.001103 0.003219 0.000201 0.001726

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.54 -3.53 2.32 6.69 7.54 -2.30 -5.15 -4.45 0.92 1.73 -3.30 -7.70 -3.10 2.47 5.07
Bud Neck -0.05 5.27 4.43 9.39 -6.41 -10.75 14.49 10.11 16.76 -6.32 -10.88 15.02 10.22 18.84 -8.64
Bud Site -1.40 2.60 1.47 5.81 -2.94 -8.79 2.82 -5.99 1.11 -7.47 -10.42 2.08 -5.26 4.71 -6.54
Cell Periphery 6.50 6.54 1.23 -2.86 -2.90 0.23 -1.70 0.58 0.59 3.16 1.76 0.87 -1.99 -2.80 -2.45
Cytoplasm -1.88 1.46 -9.91 -11.57 -16.95 7.68 4.14 -4.33 -10.76 -6.27 9.51 13.59 -4.89 -14.56 -17.40
Cytoplasmic Foci 2.86 18.71 16.10 26.55 -18.00 2.57 38.74 39.00 32.06 -3.60 1.21 47.43 40.77 40.20 -15.92
Eisosomes 6.43 7.21 6.24 -0.61 -4.91 -0.49 6.44 15.00 11.79 5.21 3.66 10.89 7.09 4.58 -2.67
Endoplasmic Reticulum -2.85 -13.09 -1.20 -0.20 7.14 1.30 -9.27 -7.58 -10.60 7.65 -1.24 -16.54 -2.80 -2.46 10.73
Endosome -4.77 4.46 3.35 12.56 -4.15 -7.44 6.41 5.58 10.87 -0.54 -11.91 8.63 4.79 15.44 -3.65
Golgi 0.71 4.08 3.66 5.41 -3.94 -4.11 11.19 3.80 6.25 -2.34 -4.55 10.26 5.54 8.20 -4.40
Lipid Particles 4.22 6.52 6.23 5.64 -2.39 7.32 10.50 12.60 10.02 4.56 6.65 12.58 12.57 8.92 0.25
Mitochondria 3.52 5.40 5.24 7.21 -3.08 -3.55 10.14 5.61 8.19 -3.12 -1.89 10.24 8.05 11.18 -4.33
Mitotic Spindle -1.81 0.77 -1.19 1.01 -3.82 -4.05 1.90 -6.11 -3.22 -7.12 -4.88 1.51 -6.27 -1.78 -7.81
None -2.16 -3.29 -6.75 -4.95 -5.73 1.01 -0.64 -2.58 -2.90 -2.20 0.12 -1.38 -6.55 -6.15 -6.00
Nuclear Periphery -2.24 -16.94 -3.28 -1.33 11.80 -1.00 -9.10 -9.02 -6.43 0.83 -1.59 -18.52 -6.90 -4.90 11.32
Nuclear Periphery Foci -3.09 -9.86 -3.17 1.62 8.75 0.58 -8.67 -8.58 -8.20 7.26 -1.66 -12.83 -7.51 -4.07 10.85
Nucleolus -3.65 2.59 -0.78 3.60 -6.91 -1.12 4.94 3.37 5.25 -2.20 -3.24 5.25 1.24 5.09 -6.86
Nucleus 2.81 -21.79 -10.41 -23.45 17.67 8.06 -9.71 -4.37 -11.01 7.47 11.74 -24.42 -9.94 -21.57 18.96
Peroxisomes 2.06 6.81 6.41 10.49 -4.98 -8.77 13.71 11.12 14.53 -3.41 -8.84 14.75 11.79 17.62 -6.12
Vacuole -13.23 -13.54 -18.80 -2.48 2.42 -15.52 -11.96 -22.61 -11.10 2.15 -19.99 -17.79 -29.75 -10.65 2.77
Vacuole Periphery -1.08 3.16 1.92 4.32 -3.12 -3.86 8.89 -0.73 2.65 -3.21 -5.71 8.34 -0.59 4.50 -4.39
Standard name
Human Ortholog
Description 5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis
Localization
Cell Percentages cytoplasm (10%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fau1

Fau1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fau1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available