Standard name
Human Ortholog
Description Putative assembly factor for cytochrome c oxidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; has similarity to human MCUR1/CCDC90A
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0.06 0.05 0.07 0 0 0 0 0.25 0.11 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.32 0.55 0.46 0.28 0.29 0.22 – 0.2 0.2 0.35 0.19 0.46 0.66 0.68 0.34 0.31 0.46 0 0.09 0.08 0.06 0.06 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Endosome 0.05 0 0 0.09 0.2 0 0.17 – 0.06 0 0.05 0 0.15 0.07 0.11 0.13 0.25 0.13 0.12 0.05 0.07 0.07 0.09 0.08
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Mitochondria 0.68 0.68 0.24 0.33 0.36 0.64 0.52 – 0.7 0.77 0.55 0.78 0.31 0.2 0.15 0.17 0 0.09 0.67 0.69 0.7 0.65 0.61 0.6
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0.1 0.2 0 0.06 – 0.05 0 0 0 0.07 0 0 0 0.06 0 0 0 0 0 0 0
SpindlePole 0.06 0 0.12 0.11 0.11 0.05 0.13 – 0.09 0.1 0.06 0.12 0 0 0 0.12 0.06 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0 0.05 0.06 0.11 0.09 0.09 – 0.06 0 0 0 0.15 0.13 0.23 0.17 0.06 0.15 0 0.06 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 1 0 – 0 0 0 0 1 0 0 1 0 0 2 2 1 6 9 14
Bud 4 3 1 3 3 10 11 – 7 18 3 11 0 0 3 4 4 5 3 0 0 5 3 9
Bud Neck 1 0 3 4 1 3 2 – 2 1 0 0 0 0 0 0 1 0 2 1 0 5 5 7
Bud Site 0 0 0 0 0 1 1 – 1 3 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 2 3 6 0 4 – 3 1 3 1 3 3 1 2 0 0 1 0 1 1 2 2
Cytoplasm 35 36 51 108 103 73 91 – 40 65 23 28 113 258 307 32 5 21 8 11 11 16 25 27
Endoplasmic Reticulum 2 4 0 0 0 0 0 – 2 0 0 0 2 3 3 4 0 4 1 1 2 1 2 7
Endosome 11 1 4 21 75 11 69 – 11 9 3 0 37 28 48 12 4 6 31 5 9 20 35 32
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 1 1 6 1 0 5 9 10
Mitochondria 141 77 22 79 133 160 216 – 139 247 36 114 75 79 67 16 0 4 177 90 99 179 248 244
Nucleus 2 2 0 0 5 3 14 – 5 6 2 5 0 2 1 2 0 0 0 0 0 0 0 1
Nuclear Periphery 3 1 0 1 0 0 1 – 0 0 0 1 0 0 0 2 0 0 3 0 2 1 1 2
Nucleolus 1 0 0 1 1 0 1 – 1 0 0 0 0 1 0 0 0 0 2 0 0 0 2 2
Peroxisomes 4 1 5 23 72 3 23 – 10 9 1 6 18 3 7 4 1 0 7 1 1 11 16 8
SpindlePole 13 3 11 27 42 12 52 – 17 32 4 17 4 7 4 11 1 4 5 1 3 5 14 14
Vac/Vac Membrane 28 3 5 13 41 22 39 – 12 10 1 5 37 52 102 16 1 7 5 7 3 8 19 16
Unique Cell Count 206 113 93 236 368 250 414 199 319 65 147 245 388 450 93 16 46 265 131 142 275 406 411
Labelled Cell Count 245 134 105 283 482 299 524 250 401 77 193 290 436 543 108 18 52 265 131 142 275 406 411


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.1 5.2 4.7 5.5 4.1 4.7 – 4.2 4.1 4.8 4.2 5.1 4.6 5.1 5.2 6.1 5.9 4.6 4.7 4.9
Std Deviation (1e-4) 1.0 1.4 2.1 1.5 1.4 2.1 1.5 – 2.0 1.4 2.3 1.6 1.9 1.5 1.7 1.2 2.4 1.6 1.6 1.9 2.2
Intensity Change (Log2) – – – -0.13 0.09 -0.33 -0.13 – -0.29 -0.34 -0.11 -0.32 -0.02 -0.17 -0.01 0.02 0.23 0.18 -0.17 -0.15 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 2.5 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7676 -0.4058 -0.6529 -0.5942 -1.117 -0.5521 0.1778 0.7438 0.6239 0.6056 1.068 0.8133 2.0527 3.2371 2.6876 2.8763 2.591 2.6745
Actin 0.0454 0.046 0.0337 0.0267 0.0206 0.0176 0.0227 0.0083 0.0169 0.0083 0.009 0.0083 0.0312 0.0461 0.0319 0.0776 0.0542 0.0098
Bud 0.0017 0.0055 0.0025 0.0056 0.0015 0.0009 0.0009 0.0028 0.0017 0.0011 0.0033 0.0011 0.0013 0.0049 0.0024 0.0047 0.002 0.0007
Bud Neck 0.0028 0.0054 0.0034 0.0015 0.0007 0.006 0.0015 0.0011 0.0073 0.0003 0.0015 0.0035 0.0051 0.0016 0.0022 0.004 0.0014 0.004
Bud Periphery 0.0025 0.0089 0.0112 0.0094 0.0048 0.0018 0.0019 0.0082 0.0035 0.0022 0.0073 0.0029 0.004 0.011 0.0065 0.0109 0.0046 0.0022
Bud Site 0.012 0.0796 0.0108 0.0023 0.0019 0.0011 0.0043 0.0185 0.0126 0.0012 0.0039 0.0011 0.0136 0.0453 0.0139 0.013 0.0026 0.0011
Cell Periphery 0.0012 0.0077 0.0017 0.0004 0.0005 0.0009 0.001 0.0021 0.001 0.0003 0.0012 0.001 0.0037 0.0044 0.0021 0.002 0.0011 0.0007
Cytoplasm 0.0354 0.0604 0.0283 0.0184 0.0518 0.0283 0.0204 0.0329 0.0331 0.0271 0.0708 0.0299 0.0393 0.0495 0.0298 0.0073 0.0166 0.0251
Cytoplasmic Foci 0.0815 0.0925 0.0828 0.077 0.0844 0.0999 0.1039 0.0887 0.0846 0.0356 0.0738 0.0521 0.0901 0.0716 0.068 0.0736 0.0811 0.0595
Eisosomes 0.0017 0.0045 0.0034 0.0005 0.0012 0.0006 0.0009 0.0009 0.0012 0.0002 0.0002 0.0004 0.0015 0.002 0.0017 0.0008 0.0043 0.0003
Endoplasmic Reticulum 0.0103 0.0034 0.0021 0.0015 0.0031 0.0012 0.0041 0.0019 0.0036 0.0012 0.0021 0.0033 0.0129 0.0092 0.0047 0.0044 0.0042 0.0023
Endosome 0.0976 0.0628 0.0666 0.0567 0.1504 0.0844 0.1688 0.1065 0.134 0.0548 0.1473 0.094 0.1644 0.1117 0.1093 0.0911 0.1574 0.1125
Golgi 0.0478 0.0529 0.0524 0.0352 0.0418 0.0651 0.0869 0.056 0.0664 0.0325 0.0618 0.0643 0.0922 0.0833 0.0713 0.0764 0.1143 0.0715
Lipid Particles 0.0608 0.0138 0.0249 0.0163 0.0201 0.0284 0.0482 0.0116 0.0256 0.0233 0.0419 0.0081 0.0322 0.0129 0.0154 0.0143 0.0125 0.0155
Mitochondria 0.3775 0.3667 0.5112 0.4846 0.4356 0.5429 0.3922 0.5606 0.4865 0.7197 0.4516 0.6673 0.3683 0.4377 0.5286 0.5098 0.4401 0.6125
None 0.1251 0.1163 0.0828 0.2283 0.079 0.0469 0.0727 0.0389 0.0457 0.0368 0.0106 0.0153 0.0678 0.0588 0.0656 0.0394 0.0487 0.0344
Nuclear Periphery 0.0137 0.0012 0.0034 0.0008 0.003 0.0031 0.0015 0.0008 0.0016 0.0018 0.0014 0.007 0.0045 0.0029 0.0028 0.0008 0.0056 0.0012
Nucleolus 0.0046 0.0018 0.0011 0.001 0.0011 0.0014 0.0007 0.0023 0.0006 0.0003 0.0003 0.0005 0.0013 0.0006 0.001 0.0005 0.0006 0.0004
Nucleus 0.013 0.0023 0.0088 0.0009 0.002 0.0016 0.0007 0.0017 0.0008 0.0006 0.0006 0.0009 0.0016 0.0012 0.001 0.0007 0.0011 0.0006
Peroxisomes 0.0449 0.0565 0.0534 0.0224 0.0775 0.0558 0.0453 0.0324 0.0566 0.0321 0.0996 0.0285 0.0437 0.0345 0.029 0.0454 0.0352 0.0316
Punctate Nuclear 0.0057 0.002 0.0026 0.0061 0.0053 0.0044 0.0033 0.0007 0.0016 0.0028 0.0028 0.0004 0.0049 0.0007 0.0009 0.0006 0.0046 0.0035
Vacuole 0.009 0.0064 0.0092 0.0023 0.0093 0.0039 0.0131 0.0171 0.0093 0.0128 0.0061 0.0055 0.0112 0.0062 0.0077 0.0156 0.004 0.0065
Vacuole Periphery 0.0057 0.0034 0.0038 0.002 0.0044 0.0037 0.0048 0.0059 0.0056 0.0051 0.0029 0.0045 0.0052 0.004 0.0043 0.0071 0.0037 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.3352 17.388 11.7297 16.9985 13.7559 10.6038 11.9871 14.0235 16.048 18.6141
Translational Efficiency 1.3672 0.9674 1.13 1.128 1.6112 2.9268 1.7729 1.8031 1.4703 1.4464

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
709 804 1038 387 1586 2156 1185 1377 2295 2960 2223 1764

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 578.31 670.04 866.83 749.36 684.13 772.85 815.52 784.40 651.44 744.92 839.48 776.71
Standard Deviation 86.74 123.97 108.34 100.16 76.36 97.12 105.06 107.57 93.52 114.61 109.63 106.97
Intensity Change Log 2 — 0.212404 0.583906 0.373816 — 0.175918 0.253450 0.197319 — 0.192746 0.414263 0.280858

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000895 0.001796 0.001539 0.000787 0.000622 0.001769 0.005188 0.004393 0.000706 0.001776 0.003484 0.003601
Bud Neck 0.036812 0.021702 0.027327 0.040128 0.035957 0.021626 0.031901 0.041163 0.036221 0.021646 0.029765 0.040936
Bud Site 0.022346 0.044128 0.023279 0.017403 0.019776 0.023153 0.097054 0.048500 0.020570 0.028850 0.062606 0.041678
Cell Periphery 0.001429 0.000513 0.000237 0.000443 0.000235 0.000314 0.000226 0.000804 0.000604 0.000368 0.000231 0.000725
Cytoplasm 0.079367 0.033898 0.112093* 0.223315* 0.096003 0.027612 0.201411* 0.276844* 0.090863 0.029319 0.159705* 0.265100*
Cytoplasmic Foci 0.157094 0.132405 0.124932 0.154854 0.270432* 0.120548 0.169689 0.118567 0.235418* 0.123768 0.148790 0.126528
Eisosomes 0.000614 0.000150 0.000127 0.000280 0.000210 0.000163 0.000124 0.000344 0.000334 0.000159 0.000125 0.000330
Endoplasmic Reticulum 0.002923 0.000770 0.008565 0.003232 0.002013 0.000362 0.003968 0.003760 0.002294 0.000472 0.006115 0.003644
Endosome 0.028768 0.121291 0.091427 0.039512 0.069933 0.080696 0.060874 0.030767 0.057216 0.091723 0.075140 0.032686
Golgi 0.048389 0.113059* 0.024492 0.009567 0.070451 0.126706* 0.030843 0.013998 0.063635 0.122999* 0.027877 0.013026
Lipid Particles 0.023316 0.011480 0.021501 0.025636 0.020344 0.005895 0.005378 0.017795 0.021262 0.007412 0.012906 0.019515
Mitochondria 0.486997* 0.353315* 0.066239 0.029885 0.287407* 0.477164* 0.045060 0.034386 0.349067* 0.443524* 0.054949 0.033399
Mitotic Spindle 0.001237 0.020237 0.080152 0.013417 0.010333 0.018879 0.059400 0.032353 0.007523 0.019248 0.069090 0.028199
None 0.001683 0.004478 0.002543 0.005397 0.001311 0.000903 0.001581 0.008046 0.001426 0.001874 0.002030 0.007465
Nuclear Periphery 0.000526 0.000249 0.007636 0.002241 0.000338 0.000091 0.001112 0.002009 0.000396 0.000134 0.004158 0.002060
Nuclear Periphery Foci 0.000346 0.000467 0.007456 0.001728 0.000266 0.000147 0.001639 0.001195 0.000290 0.000234 0.004355 0.001312
Nucleolus 0.002039 0.004635 0.017018 0.004799 0.001366 0.001400 0.002223 0.005701 0.001574 0.002279 0.009131 0.005504
Nucleus 0.029263 0.013605 0.136569* 0.247820* 0.020964 0.007085 0.067977 0.175964* 0.023528 0.008856 0.100005 0.191728*
Peroxisomes 0.044732 0.074824 0.023819 0.036439 0.042329 0.049774 0.064555 0.040465 0.043071 0.056578 0.045534 0.039582
Vacuole 0.017221 0.029983 0.191382 0.136154 0.038940 0.023880 0.140952 0.137255 0.032230 0.025538 0.164500 0.137013
Vacuole Periphery 0.014005 0.017016 0.031668 0.006962 0.010772 0.011835 0.008844 0.005690 0.011771 0.013242 0.019501 0.005969

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.95 -1.78 0.33 2.57 2.58 -2.59 -9.00 -5.13 -2.72 1.44 -3.07 -8.83 -4.93 -2.35 0.41
Bud Neck 3.34 2.21 0.16 -2.74 -1.73 4.89 1.63 -0.57 -5.42 -2.23 5.91 2.83 -0.42 -6.10 -3.17
Bud Site -4.18 -0.14 1.30 5.72 1.87 -1.66 -15.42 -7.02 -5.90 8.63 -3.93 -12.98 -5.95 -3.04 5.77
Cell Periphery 2.74 3.68 3.09 1.00 -2.67 -2.00 0.82 -4.54 -3.51 -4.77 2.20 3.72 -0.02 -2.94 -5.14
Cytoplasm 6.80 -4.70 -10.94 -15.40 -8.02 14.99 -13.46 -22.21 -34.47 -7.11 16.37 -12.75 -25.08 -37.54 -13.06
Cytoplasmic Foci 2.69 4.48 0.72 -1.58 -3.06 23.07 14.56 23.13 1.73 8.36 20.87 16.40 19.93 0.93 4.86
Eisosomes 4.74 4.98 3.49 -5.94 -7.48 2.52 6.86 -2.46 -3.65 -5.08 5.28 6.60 1.04 -4.56 -6.10
Endoplasmic Reticulum 2.70 -6.13 -0.38 -3.47 6.01 4.14 -4.45 -3.11 -8.78 0.87 4.93 -8.48 -2.69 -9.30 5.99
Endosome -14.65 -13.02 -1.71 12.17 9.38 -2.85 3.24 13.03 17.14 9.15 -10.58 -4.27 10.73 21.14 14.76
Golgi -9.37 6.10 9.09 16.71 4.37 -11.74 11.52 17.43 28.79 6.16 -15.03 13.55 19.46 33.19 7.26
Lipid Particles 3.60 0.71 -0.07 -3.06 -0.66 8.69 9.32 2.23 -5.58 -6.06 9.18 5.45 2.05 -6.08 -2.71
Mitochondria 7.47 29.89 31.95* 24.94* 5.46 -17.56 29.26 31.46* 56.89* 3.10 -9.82 39.43* 43.06* 60.87* 6.57
Mitotic Spindle -8.32 -13.93 -2.95 1.60 9.68 -4.43 -10.47 -6.48 -3.80 4.95 -7.76 -16.69 -7.49 -2.94 9.38
None -2.10 -2.00 -1.89 0.01 -1.36 1.47 -0.71 -4.88 -5.26 -4.65 -1.18 -2.17 -5.21 -4.39 -4.60
Nuclear Periphery 2.06 -8.82 -3.68 -4.40 5.90 5.66 -3.51 -6.88 -7.88 -2.30 5.18 -9.49 -7.69 -8.97 4.42
Nuclear Periphery Foci -1.13 -5.98 -2.94 -2.61 4.51 3.44 -6.31 -5.69 -6.31 1.37 0.81 -7.17 -6.24 -6.50 5.04
Nucleolus -2.14 -7.63 -2.33 0.34 6.35 -0.13 -3.17 -5.43 -5.27 -4.04 -1.76 -8.31 -5.70 -4.27 3.51
Nucleus 3.38 -14.20 -16.15 -17.71 -7.10 6.85 -11.81 -26.08 -29.08 -14.16 7.42 -19.03 -30.19 -33.71 -12.88
Peroxisomes -4.88 5.15 2.09 6.25 -1.90 -2.26 -4.18 1.71 3.62 5.18 -4.61 -0.06 2.49 6.39 2.36
Vacuole -3.87 -26.11 -13.73 -12.36 2.88 5.84 -20.11 -21.38 -24.80 -1.21 3.19 -31.52 -25.81 -27.63 1.60
Vacuole Periphery -1.56 -5.76 4.30 5.84 8.83 -1.27 3.08 7.85 8.29 3.66 -1.78 -4.76 8.96 10.36 10.28
Standard name
Human Ortholog
Description Putative assembly factor for cytochrome c oxidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; has similarity to human MCUR1/CCDC90A
Localization
Cell Percentages mitochondrion (72%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Fmp32

Fmp32


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fmp32-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available