Standard name | |
---|---|
Human Ortholog | |
Description | Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 6 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 3 | 5 | 3 | 2 | 1 | 3 | 2 | 3 | 4 |
Bud | 0 | 4 | 8 | 2 | 0 | 4 | 4 | 4 | 9 | – | 16 | 5 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | – | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 13 | 3 | 4 | 5 | 2 | 9 | 3 | – | 3 | 8 | 3 | 9 | 0 | 21 | 16 | 12 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 103 | 202 | 321 | 187 | 355 | 379 | 504 | 527 | 418 | – | 354 | 396 | 133 | 271 | 285 | 474 | 236 | 217 | 97 | 201 | 291 | 63 | 153 | 134 |
Endoplasmic Reticulum | 8 | 11 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 5 | 2 | 3 | 2 | 14 | 12 | 9 | 6 | 8 | 8 | 18 | 31 | 35 |
Endosome | 1 | 5 | 8 | 2 | 2 | 1 | 4 | 4 | 1 | – | 1 | 1 | 1 | 3 | 0 | 4 | 7 | 6 | 9 | 11 | 4 | 0 | 5 | 8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 10 | 2 | 2 | 2 | 0 | 2 | 4 |
Mitochondria | 10 | 1 | 2 | 18 | 22 | 61 | 84 | 66 | 147 | – | 178 | 77 | 2 | 0 | 1 | 6 | 3 | 2 | 5 | 6 | 38 | 8 | 17 | 24 |
Nucleus | 2 | 0 | 0 | 0 | 5 | 4 | 6 | 6 | 6 | – | 7 | 3 | 0 | 1 | 1 | 8 | 4 | 2 | 0 | 0 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 3 | 0 | 2 | 1 | 2 | 6 | 2 | – | 2 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 1 | 13 | 5 | 10 | 8 | 4 | 11 | 7 | – | 4 | 3 | 1 | 1 | 0 | 26 | 19 | 28 | 4 | 18 | 17 | 10 | 12 | 18 |
Unique Cell Count | 118 | 222 | 348 | 200 | 378 | 418 | 551 | 580 | 492 | 455 | 461 | 139 | 280 | 289 | 518 | 279 | 261 | 135 | 257 | 380 | 110 | 232 | 247 | |
Labelled Cell Count | 124 | 229 | 377 | 217 | 402 | 465 | 614 | 637 | 595 | 568 | 509 | 144 | 291 | 290 | 559 | 309 | 289 | 135 | 257 | 380 | 110 | 232 | 247 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.7 | 7.8 | 7.1 | 6.5 | 6.5 | 5.1 | 5.1 | 5.4 | 4.2 | – | 3.8 | 4.2 | 7.3 | 7.4 | 7.0 | 12.8 | 13.3 | 13.1 | 6.9 | 6.8 | 7.4 |
Std Deviation (1e-4) | 1.1 | 1.0 | 0.8 | 1.1 | 1.1 | 0.9 | 1.3 | 1.4 | 1.2 | – | 0.9 | 0.7 | 1.2 | 1.1 | 1.1 | 2.1 | 2.8 | 1.9 | 1.2 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.12 | -0.47 | -0.46 | -0.38 | -0.74 | – | -0.9 | -0.75 | 0.04 | 0.06 | -0.01 | 0.86 | 0.92 | 0.89 | -0.03 | -0.06 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | -1.5 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.5 | -2.4 | -2.3 | -3.8 | -2.1 | 0 | – | 0 | 0 | 0 | -0.4 | -3.3 | 0.2 | 1.2 | 0.5 |
Cytoplasm | 0.5 | 0.9 | -0.8 | -0.4 | -0.7 | -3.2 | – | -5.5 | -2.8 | 1.4 | 2.4 | 3.7 | -0.4 | -3.0 | -3.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.8 | 2.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.1 | 3.9 | 7.0 | 7.3 | 6.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.8 | -0.8 | -1.5 | -3.2 | -1.7 | -2.2 | – | -2.8 | -3.1 | 0 | -2.9 | -3.3 | 0.9 | 1.7 | 3.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4297 | 4.8909 | 4.133 | 4.0993 | 3.1415 | 4.0 | 2.3654 | 3.0525 | 2.8976 | 2.5414 | 2.4976 | 2.8347 | 1.7297 | 2.6773 | 2.3473 | 2.1865 | 2.377 | 2.2622 |
Actin | 0.0713 | 0.0005 | 0.0274 | 0.0154 | 0.0004 | 0.0096 | 0.0232 | 0.0028 | 0.0044 | 0.0007 | 0.002 | 0.0066 | 0.0086 | 0.001 | 0.0068 | 0.0129 | 0.0002 | 0.0029 |
Bud | 0.0007 | 0.0003 | 0.001 | 0.001 | 0.0005 | 0.0002 | 0.0012 | 0.0025 | 0.0018 | 0.0017 | 0.0005 | 0.0011 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.001 | 0.0002 |
Bud Neck | 0.0028 | 0.0002 | 0.0007 | 0.001 | 0.0004 | 0.0021 | 0.0072 | 0.0003 | 0.0011 | 0.0007 | 0.0008 | 0.0018 | 0.0017 | 0.0002 | 0.0003 | 0.0007 | 0.0005 | 0.0041 |
Bud Periphery | 0.0009 | 0.0001 | 0.001 | 0.0009 | 0.0003 | 0.0002 | 0.0013 | 0.0025 | 0.0019 | 0.0012 | 0.0004 | 0.0034 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0011 | 0.0001 |
Bud Site | 0.014 | 0.0021 | 0.0051 | 0.0009 | 0.0002 | 0.0002 | 0.0062 | 0.0115 | 0.0115 | 0.0006 | 0.0009 | 0.0005 | 0.0011 | 0.0021 | 0.0012 | 0.001 | 0.0012 | 0.0005 |
Cell Periphery | 0.0018 | 0.0008 | 0.0006 | 0.0009 | 0.0002 | 0.0002 | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 |
Cytoplasm | 0.5921 | 0.8997 | 0.7905 | 0.7719 | 0.7835 | 0.8677 | 0.6945 | 0.8761 | 0.8026 | 0.7629 | 0.6793 | 0.8375 | 0.7872 | 0.8708 | 0.8642 | 0.8151 | 0.7387 | 0.8874 |
Cytoplasmic Foci | 0.0547 | 0.0189 | 0.0243 | 0.0237 | 0.0212 | 0.0247 | 0.0551 | 0.0245 | 0.0433 | 0.0694 | 0.0855 | 0.0405 | 0.0428 | 0.0323 | 0.0321 | 0.0359 | 0.0635 | 0.0299 |
Eisosomes | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0148 | 0.0039 | 0.0096 | 0.0065 | 0.0062 | 0.0121 | 0.0112 | 0.0044 | 0.0041 | 0.0026 | 0.0037 | 0.0041 | 0.0351 | 0.0105 | 0.0051 | 0.0037 | 0.0037 | 0.0055 |
Endosome | 0.0484 | 0.0238 | 0.0438 | 0.0591 | 0.0544 | 0.0327 | 0.0896 | 0.0372 | 0.0728 | 0.1103 | 0.1376 | 0.0683 | 0.061 | 0.0533 | 0.0564 | 0.0913 | 0.0864 | 0.0483 |
Golgi | 0.0151 | 0.0015 | 0.012 | 0.0275 | 0.0039 | 0.005 | 0.0189 | 0.0033 | 0.01 | 0.0075 | 0.0149 | 0.0079 | 0.0097 | 0.0034 | 0.0051 | 0.0034 | 0.0025 | 0.0072 |
Lipid Particles | 0.0214 | 0.0004 | 0.0115 | 0.0078 | 0.0001 | 0.0004 | 0.0065 | 0.0002 | 0.0009 | 0.0029 | 0.0065 | 0.0017 | 0.0023 | 0.0002 | 0.0005 | 0.0013 | 0.0001 | 0.0006 |
Mitochondria | 0.005 | 0.0002 | 0.0044 | 0.0063 | 0.0011 | 0.0019 | 0.0187 | 0.002 | 0.0049 | 0.0016 | 0.0269 | 0.0075 | 0.0021 | 0.0007 | 0.0015 | 0.0009 | 0.0006 | 0.0012 |
None | 0.1083 | 0.0422 | 0.0424 | 0.0576 | 0.1222 | 0.0338 | 0.028 | 0.0224 | 0.0226 | 0.0062 | 0.0037 | 0.0081 | 0.0194 | 0.0164 | 0.0165 | 0.0098 | 0.0265 | 0.0059 |
Nuclear Periphery | 0.0036 | 0.0005 | 0.0011 | 0.0012 | 0.0003 | 0.0028 | 0.0058 | 0.0005 | 0.0008 | 0.0006 | 0.0043 | 0.0028 | 0.0079 | 0.0006 | 0.0009 | 0.0017 | 0.001 | 0.0005 |
Nucleolus | 0.0014 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0002 | 0 |
Nucleus | 0.0013 | 0.0006 | 0.0008 | 0.0011 | 0.0004 | 0.0011 | 0.0073 | 0.0011 | 0.0012 | 0.0012 | 0.0039 | 0.0008 | 0.0034 | 0.0008 | 0.0008 | 0.0016 | 0.0014 | 0.0006 |
Peroxisomes | 0.0246 | 0.0001 | 0.0132 | 0.0004 | 0.0001 | 0.0002 | 0.0108 | 0.0014 | 0.0043 | 0.0123 | 0.0046 | 0.0013 | 0.0024 | 0.0002 | 0.0004 | 0.0011 | 0.0003 | 0.0008 |
Punctate Nuclear | 0.0078 | 0.0002 | 0.001 | 0.0003 | 0.0001 | 0.0007 | 0.0033 | 0.0004 | 0.0005 | 0.0023 | 0.0144 | 0.0006 | 0.0054 | 0.0005 | 0.0004 | 0.0035 | 0.0003 | 0.0003 |
Vacuole | 0.0081 | 0.0033 | 0.0082 | 0.0137 | 0.0034 | 0.0033 | 0.0074 | 0.0056 | 0.0092 | 0.0119 | 0.0069 | 0.0037 | 0.0072 | 0.0052 | 0.0057 | 0.012 | 0.0639 | 0.003 |
Vacuole Periphery | 0.001 | 0.0004 | 0.0012 | 0.0024 | 0.0011 | 0.0009 | 0.002 | 0.0011 | 0.0018 | 0.0031 | 0.0025 | 0.0015 | 0.0014 | 0.001 | 0.0013 | 0.0028 | 0.0067 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.9215 | 13.6137 | 19.2856 | 14.3659 | 21.8391 | 12.4531 | 15.329 | 14.6393 | 13.4463 | 14.132 |
Translational Efficiency | 3.211 | 2.5941 | 1.5689 | 2.0235 | 1.336 | 1.7882 | 1.5156 | 1.1998 | 1.2782 | 1.6518 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2387 | 1774 | 1458 | 1675 | 1600 | 1794 | 1477 | 3118 | 3987 | 3568 | 2935 | 4793 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 830.68 | 909.08 | 1098.20 | 1160.86 | 855.93 | 875.97 | 1052.57 | 1293.21 | 840.81 | 892.43 | 1075.24 | 1246.96 |
Standard Deviation | 96.36 | 128.78 | 177.45 | 157.57 | 101.69 | 136.27 | 135.76 | 173.61 | 99.30 | 133.63 | 159.49 | 179.63 |
Intensity Change Log 2 | — | 0.130114 | 0.402776 | 0.482829 | — | 0.033389 | 0.298351 | 0.595392 | — | 0.081838 | 0.350727 | 0.541050 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000209 | 0.000212 | 0.001611 | 0.000546 | 0.000303 | 0.000325 | 0.000555 | 0.000273 | 0.000247 | 0.000269 | 0.001079 | 0.000368 |
Bud Neck | 0.006333 | 0.003657 | 0.007538 | 0.002770 | 0.004188 | 0.006785 | 0.006828 | 0.002891 | 0.005472 | 0.005230 | 0.007181 | 0.002849 |
Bud Site | 0.019389 | 0.022245 | 0.016595 | 0.033553 | 0.016022 | 0.026167 | 0.017367 | 0.021996 | 0.018038 | 0.024217 | 0.016984 | 0.026035 |
Cell Periphery | 0.000135 | 0.000093 | 0.000223 | 0.000124 | 0.000178 | 0.000111 | 0.000084 | 0.000095 | 0.000153 | 0.000102 | 0.000153 | 0.000105 |
Cytoplasm | 0.537293* | 0.550583* | 0.382387* | 0.574395* | 0.502234* | 0.438252* | 0.284213* | 0.785431* | 0.523224* | 0.494103* | 0.332982* | 0.711680* |
Cytoplasmic Foci | 0.288890* | 0.281787* | 0.137016 | 0.106816 | 0.343262* | 0.309853* | 0.233174* | 0.043456 | 0.310709* | 0.295899* | 0.185406* | 0.065598 |
Eisosomes | 0.000077 | 0.000031 | 0.000111 | 0.000009 | 0.000100 | 0.000069 | 0.000051 | 0.000006 | 0.000086 | 0.000050 | 0.000080 | 0.000007 |
Endoplasmic Reticulum | 0.002263 | 0.001685 | 0.003780 | 0.000564 | 0.004565 | 0.002071 | 0.002573 | 0.000077 | 0.003187 | 0.001879 | 0.003173 | 0.000247 |
Endosome | 0.026942 | 0.029846 | 0.051114 | 0.035171 | 0.026997 | 0.054992 | 0.127357 | 0.012840 | 0.026964 | 0.042489 | 0.089482 | 0.020644 |
Golgi | 0.034449 | 0.022241 | 0.003281 | 0.010711 | 0.023472 | 0.027510 | 0.006393 | 0.008300 | 0.030044 | 0.024890 | 0.004847 | 0.009143 |
Lipid Particles | 0.003483 | 0.001835 | 0.014133 | 0.002357 | 0.004189 | 0.005161 | 0.010053 | 0.001106 | 0.003766 | 0.003507 | 0.012079 | 0.001543 |
Mitochondria | 0.004521 | 0.002616 | 0.000581 | 0.000794 | 0.002360 | 0.004966 | 0.000694 | 0.001073 | 0.003654 | 0.003798 | 0.000638 | 0.000976 |
Mitotic Spindle | 0.000706 | 0.001791 | 0.056585 | 0.010560 | 0.002384 | 0.002602 | 0.030940 | 0.003193 | 0.001379 | 0.002199 | 0.043679 | 0.005767 |
None | 0.001567 | 0.001005 | 0.002074 | 0.000234 | 0.000932 | 0.000451 | 0.001146 | 0.000133 | 0.001312 | 0.000726 | 0.001607 | 0.000168 |
Nuclear Periphery | 0.000066 | 0.000051 | 0.001942 | 0.000143 | 0.000067 | 0.000500 | 0.000848 | 0.000034 | 0.000066 | 0.000277 | 0.001391 | 0.000072 |
Nuclear Periphery Foci | 0.000205 | 0.000307 | 0.007057 | 0.000712 | 0.000583 | 0.000614 | 0.004994 | 0.000117 | 0.000357 | 0.000461 | 0.006019 | 0.000325 |
Nucleolus | 0.000296 | 0.000196 | 0.002141 | 0.000258 | 0.000233 | 0.000645 | 0.002030 | 0.000257 | 0.000271 | 0.000422 | 0.002085 | 0.000257 |
Nucleus | 0.007609 | 0.005235 | 0.086091 | 0.004542 | 0.008482 | 0.007068 | 0.023584 | 0.003189 | 0.007959 | 0.006157 | 0.054636 | 0.003662 |
Peroxisomes | 0.002420 | 0.001974 | 0.007397 | 0.003208 | 0.001802 | 0.003237 | 0.008482 | 0.002684 | 0.002172 | 0.002609 | 0.007943 | 0.002867 |
Vacuole | 0.053116 | 0.062658 | 0.216179* | 0.204488* | 0.050987 | 0.098782 | 0.234386* | 0.107803 | 0.052262 | 0.080822 | 0.225341* | 0.141591 |
Vacuole Periphery | 0.010033 | 0.009952 | 0.002163 | 0.008048 | 0.006660 | 0.009836 | 0.004251 | 0.005047 | 0.008679 | 0.009894 | 0.003214 | 0.006096 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.17 | -4.67 | -3.65 | -3.62 | 3.56 | -0.55 | -3.68 | 0.99 | 1.28 | 4.00 | -1.00 | -5.38 | -2.87 | -2.16 | 4.61 |
Bud Neck | 6.42 | -2.20 | 9.99 | 2.77 | 7.94 | -6.81 | -4.83 | 5.04 | 10.69 | 7.47 | 0.82 | -4.08 | 10.88 | 9.75 | 10.78 |
Bud Site | -2.44 | 1.29 | -6.51 | -4.74 | -6.76 | -6.85 | -0.68 | -4.87 | 3.31 | -3.44 | -6.52 | 0.83 | -7.19 | -0.51 | -6.96 |
Cell Periphery | 6.09 | -3.43 | 1.81 | -2.11 | 4.18 | 5.95 | 9.19 | 5.03 | 1.31 | -0.69 | 8.09 | 1.31 | 4.76 | 0.24 | 2.98 |
Cytoplasm | -1.52 | 15.80 | -3.54 | -2.03 | -16.63 | 6.94 | 19.99 | -35.53 | -43.32 | -53.89* | 4.61 | 25.86 | -30.94 | -33.48 | -51.93* |
Cytoplasmic Foci | 1.12 | 28.11 | 34.26 | 29.02 | 3.74 | 4.55 | 16.51 | 54.09 | 50.42 | 31.22* | 3.06 | 28.89 | 66.67 | 58.69 | 27.58 |
Eisosomes | 10.85 | -0.99 | 16.92 | 13.70 | 7.05 | 4.56 | 7.54 | 16.24 | 17.47 | 10.99 | 9.33 | 2.66 | 23.71 | 21.54 | 10.29 |
Endoplasmic Reticulum | 1.69 | -2.33 | 6.27 | 5.14 | 7.54 | 3.47 | 3.08 | 6.97 | 6.25 | 7.96 | 3.63 | 0.88 | 9.66 | 8.47 | 11.19 |
Endosome | -1.65 | -6.22 | -2.87 | -1.39 | 3.52 | -11.47 | -19.76 | 10.45 | 18.52 | 23.51* | -10.12 | -19.51 | 6.22 | 14.93 | 22.30 |
Golgi | 8.39 | 28.55 | 17.20 | 9.18 | -6.71 | -2.80 | 18.53 | 14.98 | 15.23 | -3.20 | 4.92 | 33.15 | 24.12 | 17.96 | -7.54 |
Lipid Particles | 6.73 | -9.35 | 2.68 | -3.10 | 10.24 | -1.64 | -6.56 | 11.99 | 7.15 | 10.66 | 0.74 | -11.45 | 11.06 | 6.16 | 15.16 |
Mitochondria | 4.54 | 12.35 | 9.80 | 5.46 | -0.86 | -3.52 | 4.48 | 3.35 | 6.02 | -2.45 | -0.35 | 12.36 | 10.12 | 7.82 | -2.67 |
Mitotic Spindle | -2.24 | -14.09 | -5.49 | -4.83 | 10.75 | -0.09 | -9.58 | -0.82 | -0.89 | 9.54 | -1.39 | -17.09 | -5.47 | -4.54 | 14.97 |
None | 1.52 | -0.26 | 5.46 | 2.51 | 2.79 | 1.77 | -0.43 | 3.04 | 4.95 | 4.11 | 2.57 | -0.25 | 6.52 | 3.65 | 4.30 |
Nuclear Periphery | 0.56 | -10.23 | -2.35 | -3.39 | 9.74 | -1.76 | -11.11 | 1.77 | 1.88 | 11.65 | -1.70 | -13.61 | -0.69 | 1.60 | 13.56 |
Nuclear Periphery Foci | -1.74 | -12.82 | -6.13 | -4.89 | 11.63 | -0.07 | -8.62 | 2.90 | 2.88 | 9.62 | -0.92 | -15.30 | -0.44 | 0.68 | 15.32 |
Nucleolus | 2.05 | -4.54 | 0.84 | -1.61 | 4.61 | -2.31 | -4.22 | -0.65 | 2.21 | 4.17 | -1.69 | -6.07 | 0.01 | 1.76 | 6.09 |
Nucleus | 5.99 | -18.60 | 4.91 | -0.29 | 19.14 | 1.69 | -9.34 | 10.20 | 4.61 | 12.31 | 3.91 | -20.33 | 12.52 | 4.51 | 22.12 |
Peroxisomes | 2.79 | -4.69 | -2.54 | -4.20 | 3.81 | -7.75 | -10.86 | -4.25 | 2.45 | 9.10 | -3.51 | -10.05 | -3.87 | -0.95 | 8.59 |
Vacuole | -3.39 | -27.26 | -25.71 | -23.34 | 2.65 | -11.86 | -29.66 | -17.03 | -3.13 | 18.51 | -11.44 | -40.16 | -29.39 | -18.20 | 16.81 |
Vacuole Periphery | 0.12 | 17.18 | 3.73 | 3.10 | -8.65 | -4.14 | 5.19 | 3.34 | 7.71 | -3.01 | -2.19 | 15.80 | 7.06 | 8.23 | -9.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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