Standard name | |
---|---|
Human Ortholog | |
Description | Nuclear pore protein; involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm; deletion mutation extends replicative lifespan, as does exclusion of Los1p from the nucleus in response to caloric restriction |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 |
Bud | 0 | 1 | 2 | 1 | 1 | 6 | 2 | 12 | 4 | 4 | 4 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 10 | 7 | 8 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 3 | 4 | 2 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 17 | 19 | 17 | 30 | 23 | 43 | 43 | 8 | 25 | 16 | 15 | 22 | 37 | 35 | 8 | 4 | 9 | 2 | 4 | 8 | 4 | 7 | 6 |
Endoplasmic Reticulum | 4 | 0 | 2 | 2 | 0 | 0 | 4 | 1 | 6 | 7 | 1 | 2 | 1 | 4 | 5 | 2 | 1 | 2 | 3 | 3 | 11 | 3 | 3 | 7 |
Endosome | 0 | 0 | 3 | 1 | 14 | 3 | 2 | 2 | 3 | 2 | 0 | 1 | 1 | 8 | 4 | 2 | 1 | 1 | 5 | 13 | 15 | 10 | 30 | 45 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 1 | 3 | 3 |
Mitochondria | 1 | 3 | 42 | 74 | 107 | 177 | 223 | 310 | 206 | 257 | 156 | 252 | 2 | 1 | 10 | 2 | 1 | 0 | 1 | 3 | 21 | 4 | 7 | 3 |
Nucleus | 9 | 34 | 44 | 66 | 64 | 57 | 65 | 91 | 33 | 30 | 27 | 31 | 9 | 13 | 13 | 5 | 4 | 9 | 9 | 39 | 17 | 25 | 20 | 9 |
Nuclear Periphery | 36 | 62 | 58 | 101 | 98 | 49 | 69 | 38 | 37 | 59 | 25 | 52 | 35 | 28 | 28 | 10 | 6 | 14 | 134 | 174 | 267 | 39 | 79 | 74 |
Nucleolus | 2 | 0 | 0 | 3 | 2 | 5 | 3 | 9 | 4 | 4 | 3 | 7 | 2 | 5 | 3 | 2 | 0 | 0 | 1 | 6 | 1 | 2 | 1 | 1 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 2 | 3 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 4 | 9 | 8 | 3 | 2 | 3 |
Vac/Vac Membrane | 133 | 252 | 222 | 84 | 134 | 63 | 71 | 72 | 31 | 42 | 31 | 58 | 116 | 114 | 116 | 43 | 32 | 40 | 11 | 59 | 51 | 36 | 64 | 53 |
Unique Cell Count | 158 | 300 | 331 | 266 | 349 | 283 | 368 | 447 | 248 | 317 | 196 | 302 | 163 | 179 | 181 | 64 | 43 | 61 | 181 | 333 | 418 | 145 | 230 | 221 |
Labelled Cell Count | 193 | 371 | 392 | 350 | 451 | 388 | 485 | 583 | 336 | 434 | 270 | 433 | 188 | 213 | 214 | 76 | 49 | 76 | 181 | 333 | 418 | 145 | 230 | 221 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.9 | 8.0 | 5.6 | 5.4 | 5.1 | 4.5 | 4.1 | 4.1 | 3.6 | 3.5 | 3.5 | 3.4 | 7.3 | 7.4 | 6.3 | 9.7 | 10.2 | 10.9 | 8.4 | 8.5 | 9.1 |
Std Deviation (1e-4) | 1.6 | 1.4 | 1.0 | 1.1 | 1.2 | 1.2 | 1.0 | 1.2 | 0.7 | 0.9 | 1.0 | 1.0 | 1.2 | 1.4 | 1.1 | 2.2 | 2.5 | 1.9 | 1.5 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.14 | -0.31 | -0.43 | -0.46 | -0.65 | -0.65 | -0.66 | -0.74 | 0.4 | 0.41 | 0.17 | 0.8 | 0.87 | 0.97 | 0.58 | 0.61 | 0.7 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 1.4 | 1.2 | 2.8 | 2.0 | -1.4 | 1.1 | 1.1 | -0.4 | 2.9 | 5.1 | 4.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.6 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.2 | -4.7 | -2.6 | -2.2 | 0 | -2.9 |
Nucleus | 3.6 | 1.8 | 2.3 | 1.6 | 2.6 | 0 | -1.5 | 0.2 | -1.2 | -2.6 | -2.1 | -2.1 | -1.2 | -0.7 | 0.3 |
Nuclear Periphery | 5.6 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | -0.5 | -0.6 | -0.4 | -0.6 | 1.0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -8.6 | -7.5 | -11.1 | -12.8 | -14.5 | -13.1 | -13.9 | -11.4 | -12.1 | 0.9 | -0.8 | -0.7 | 0 | 1.0 | -0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.6824 | 8.2413 | 7.4834 | 7.629 | 6.0471 | 7.4623 | 7.4914 | 9.1493 | 8.2096 | 7.2793 | 8.0358 | 8.5149 | 8.8449 | 9.6916 | 9.0237 | 9.087 | 8.4961 | 8.7151 |
Actin | 0.0833 | 0.0107 | 0.0081 | 0.0005 | 0.1296 | 0.0036 | 0.0437 | 0.0004 | 0.0118 | 0.0023 | 0.0354 | 0.0014 | 0.0264 | 0.0002 | 0.0155 | 0.0033 | 0.0034 | 0.0013 |
Bud | 0.0006 | 0.0004 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0005 | 0.0004 | 0 | 0.0002 | 0.0112 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0018 | 0.0004 | 0.0002 | 0.0001 | 0.003 | 0.0005 | 0.0039 | 0.0001 | 0.0012 | 0.0019 | 0.001 | 0.0004 | 0.0033 | 0 | 0.0006 | 0.0004 | 0.0003 | 0.0003 |
Bud Periphery | 0.0012 | 0.0003 | 0.0003 | 0 | 0.001 | 0.0005 | 0.0007 | 0 | 0.0001 | 0.0008 | 0.0012 | 0.0001 | 0.0005 | 0.0216 | 0.0005 | 0.0003 | 0.0001 | 0.0001 |
Bud Site | 0.004 | 0.0035 | 0.0003 | 0 | 0.0019 | 0.0001 | 0.007 | 0 | 0.009 | 0.0024 | 0.0018 | 0.0001 | 0.0006 | 0.0013 | 0.0014 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0018 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0079 | 0.0024 | 0.0019 | 0.0006 | 0.0011 | 0.0005 | 0.007 | 0.0007 | 0.005 | 0.008 | 0.0042 | 0.0059 | 0.0019 | 0.0013 | 0.0073 | 0.001 | 0.0015 | 0.0005 |
Cytoplasmic Foci | 0.0405 | 0.0011 | 0.0034 | 0 | 0.0104 | 0.0001 | 0.0291 | 0 | 0.0109 | 0.0129 | 0.0131 | 0.0024 | 0.009 | 0.0001 | 0.0048 | 0 | 0.0302 | 0.0024 |
Eisosomes | 0.0065 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.017 | 0.0084 | 0.0048 | 0.0028 | 0.0069 | 0.0029 | 0.0052 | 0.0032 | 0.0121 | 0.0132 | 0.0064 | 0.0049 | 0.0148 | 0.0023 | 0.0088 | 0.0086 | 0.0147 | 0.0026 |
Endosome | 0.0363 | 0.0011 | 0.0088 | 0.0005 | 0.0152 | 0.0033 | 0.0218 | 0.0005 | 0.0225 | 0.0436 | 0.0256 | 0.0113 | 0.0291 | 0.0004 | 0.0034 | 0.0016 | 0.0417 | 0.0015 |
Golgi | 0.0183 | 0.0007 | 0.003 | 0 | 0.0102 | 0.001 | 0.0139 | 0 | 0.0063 | 0.0056 | 0.0042 | 0.0009 | 0.006 | 0 | 0.0028 | 0.0001 | 0.0081 | 0.0014 |
Lipid Particles | 0.0241 | 0.0027 | 0.0053 | 0 | 0.0283 | 0.0004 | 0.0211 | 0 | 0.0155 | 0.0047 | 0.0094 | 0.0017 | 0.0046 | 0 | 0.0017 | 0.0001 | 0.0635 | 0.0012 |
Mitochondria | 0.02 | 0.0012 | 0.0044 | 0.0005 | 0.0084 | 0.0095 | 0.005 | 0.0005 | 0.0009 | 0.0669 | 0.0053 | 0.0007 | 0.0024 | 0.0004 | 0.0011 | 0.0013 | 0.0009 | 0.0006 |
None | 0.008 | 0.0002 | 0.0004 | 0.0001 | 0.0023 | 0.0002 | 0.0041 | 0.0001 | 0.0008 | 0.0115 | 0.0032 | 0.0008 | 0.001 | 0.0006 | 0.0056 | 0.0003 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.6076 | 0.8222 | 0.8285 | 0.8083 | 0.7037 | 0.8664 | 0.7181 | 0.8314 | 0.7998 | 0.7574 | 0.7386 | 0.8666 | 0.8304 | 0.8899 | 0.8403 | 0.8864 | 0.7408 | 0.8926 |
Nucleolus | 0.0021 | 0.006 | 0.0003 | 0.0004 | 0.003 | 0.0006 | 0.0014 | 0.0004 | 0.0002 | 0.0007 | 0.0026 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0006 | 0.0003 |
Nucleus | 0.0706 | 0.1211 | 0.1111 | 0.1752 | 0.034 | 0.0986 | 0.0719 | 0.1504 | 0.08 | 0.0459 | 0.1156 | 0.0905 | 0.0501 | 0.0512 | 0.0816 | 0.0773 | 0.034 | 0.0782 |
Peroxisomes | 0.0212 | 0.0006 | 0.0007 | 0 | 0.0117 | 0.0001 | 0.0121 | 0 | 0.0011 | 0.0016 | 0.0111 | 0.0003 | 0.0032 | 0 | 0.0022 | 0 | 0.0291 | 0.0075 |
Punctate Nuclear | 0.0157 | 0.0014 | 0.0021 | 0.0009 | 0.0074 | 0.0007 | 0.0236 | 0.0008 | 0.0018 | 0.0025 | 0.0096 | 0.0034 | 0.0023 | 0.0004 | 0.0024 | 0.0015 | 0.0103 | 0.0007 |
Vacuole | 0.003 | 0.0013 | 0.0014 | 0.0005 | 0.0027 | 0.0013 | 0.0021 | 0.0006 | 0.004 | 0.0073 | 0.0035 | 0.0017 | 0.0041 | 0.0016 | 0.001 | 0.0013 | 0.0033 | 0.001 |
Vacuole Periphery | 0.0091 | 0.0141 | 0.0148 | 0.0096 | 0.0179 | 0.0095 | 0.0071 | 0.011 | 0.0166 | 0.01 | 0.0077 | 0.0063 | 0.0092 | 0.017 | 0.0166 | 0.0158 | 0.0171 | 0.0077 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 37.916 | 28.9671 | 29.4724 | 41.2521 | 32.2678 | 23.7052 | 32.5346 | 31.6897 | 25.5839 | 41.0797 |
Translational Efficiency | 0.6672 | 0.7986 | 0.8132 | 0.4715 | 0.5636 | 0.7189 | 0.6506 | 0.4536 | 0.641 | 0.4766 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2507 | 1435 | 1755 | 1036 | 1511 | 1863 | 1111 | 1290 | 4018 | 3298 | 2866 | 2326 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 739.03 | 928.34 | 1082.55 | 1007.11 | 843.39 | 960.76 | 1034.03 | 1101.78 | 778.28 | 946.65 | 1063.74 | 1059.61 |
Standard Deviation | 91.75 | 185.46 | 162.39 | 169.56 | 148.05 | 276.09 | 158.75 | 155.89 | 126.69 | 241.42 | 162.71 | 168.81 |
Intensity Change Log 2 | — | 0.329020 | 0.550729 | 0.446516 | — | 0.187976 | 0.294006 | 0.385564 | — | 0.255567 | 0.419602 | 0.414351 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000053 | 0.000419 | 0.000352 | 0.001279 | 0.000247 | 0.000122 | 0.000749 | 0.000335 | 0.000126 | 0.000251 | 0.000506 | 0.000756 |
Bud Neck | 0.000691 | 0.001160 | 0.005632 | 0.008609 | 0.000620 | 0.000620 | 0.008125 | 0.006148 | 0.000664 | 0.000855 | 0.006598 | 0.007244 |
Bud Site | 0.000248 | 0.001089 | 0.000741 | 0.005791 | 0.000359 | 0.000343 | 0.008960 | 0.002040 | 0.000290 | 0.000667 | 0.003927 | 0.003711 |
Cell Periphery | 0.000074 | 0.000101 | 0.000045 | 0.000138 | 0.000062 | 0.000051 | 0.000093 | 0.000048 | 0.000069 | 0.000073 | 0.000064 | 0.000088 |
Cytoplasm | 0.113001* | 0.041632 | 0.097378 | 0.111017* | 0.042080 | 0.009236 | 0.049812 | 0.045006 | 0.086331 | 0.023332 | 0.078939 | 0.074407 |
Cytoplasmic Foci | 0.009460 | 0.003125 | 0.000536 | 0.006770 | 0.004164 | 0.002431 | 0.003028 | 0.002414 | 0.007469 | 0.002733 | 0.001502 | 0.004355 |
Eisosomes | 0.000014 | 0.000009 | 0.000005 | 0.000032 | 0.000013 | 0.000005 | 0.000014 | 0.000006 | 0.000013 | 0.000007 | 0.000008 | 0.000018 |
Endoplasmic Reticulum | 0.046217 | 0.025026 | 0.016699 | 0.021716 | 0.024456 | 0.017768 | 0.024912 | 0.016462 | 0.038034 | 0.020926 | 0.019883 | 0.018802 |
Endosome | 0.004224 | 0.004391 | 0.001630 | 0.010173 | 0.004111 | 0.002837 | 0.008058 | 0.005002 | 0.004182 | 0.003513 | 0.004121 | 0.007305 |
Golgi | 0.000360 | 0.000987 | 0.000098 | 0.007072 | 0.000908 | 0.001085 | 0.001045 | 0.001107 | 0.000566 | 0.001042 | 0.000465 | 0.003764 |
Lipid Particles | 0.031351 | 0.002869 | 0.000387 | 0.002353 | 0.009796 | 0.004579 | 0.002112 | 0.001208 | 0.023245 | 0.003835 | 0.001056 | 0.001718 |
Mitochondria | 0.007194 | 0.004352 | 0.003023 | 0.006064 | 0.004110 | 0.004646 | 0.007365 | 0.002964 | 0.006034 | 0.004518 | 0.004706 | 0.004345 |
Mitotic Spindle | 0.002646 | 0.008663 | 0.013830 | 0.089722 | 0.006318 | 0.008716 | 0.059319 | 0.096263 | 0.004027 | 0.008693 | 0.031464 | 0.093350 |
None | 0.014172 | 0.019575 | 0.003145 | 0.014863 | 0.012179 | 0.001838 | 0.005012 | 0.002192 | 0.013423 | 0.009555 | 0.003869 | 0.007836 |
Nuclear Periphery | 0.411127* | 0.370044* | 0.354412* | 0.226553* | 0.415934* | 0.460029* | 0.380880* | 0.435705* | 0.412934* | 0.420875* | 0.364672* | 0.342549* |
Nuclear Periphery Foci | 0.235320* | 0.379640* | 0.077560 | 0.128688 | 0.357991* | 0.436718* | 0.210068* | 0.246850* | 0.281451* | 0.411883* | 0.128927 | 0.194221* |
Nucleolus | 0.012062 | 0.017498 | 0.009362 | 0.016215 | 0.018334 | 0.008328 | 0.018459 | 0.007308 | 0.014421 | 0.012318 | 0.012889 | 0.011275 |
Nucleus | 0.105315 | 0.106626 | 0.407047* | 0.305052* | 0.090985 | 0.031812 | 0.152350* | 0.107418 | 0.099926 | 0.064364 | 0.308314* | 0.195444* |
Peroxisomes | 0.000306 | 0.000404 | 0.000094 | 0.001153 | 0.000489 | 0.000216 | 0.000769 | 0.000136 | 0.000375 | 0.000298 | 0.000355 | 0.000589 |
Vacuole | 0.004662 | 0.008156 | 0.007189 | 0.034121 | 0.004913 | 0.005009 | 0.053165 | 0.017567 | 0.004756 | 0.006378 | 0.025012 | 0.024940 |
Vacuole Periphery | 0.001503 | 0.004233 | 0.000834 | 0.002617 | 0.001931 | 0.003612 | 0.005705 | 0.003820 | 0.001664 | 0.003883 | 0.002722 | 0.003284 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.68 | -13.49 | -3.55 | -2.42 | -2.56 | 1.83 | -5.37 | -2.78 | -9.92 | 4.27 | -4.04 | -9.02 | -4.46 | -3.64 | -1.26 |
Bud Neck | -1.99 | -13.41 | -11.77 | -11.09 | -5.16 | -0.12 | -10.47 | -11.29 | -11.13 | 0.59 | -1.41 | -16.44 | -16.27 | -15.71 | -3.50 |
Bud Site | -1.99 | -3.44 | -7.26 | -5.73 | -6.22 | 0.06 | -5.93 | -3.71 | -3.71 | 4.11 | -1.94 | -6.50 | -7.91 | -6.80 | -0.16 |
Cell Periphery | -2.82 | 4.09 | -5.51 | -2.94 | -8.01 | 1.08 | -4.32 | 0.42 | -0.90 | 5.34 | -0.52 | 0.02 | -4.09 | -2.86 | -4.43 |
Cytoplasm | 14.45 | 3.43 | 0.39 | -11.23 | -2.40 | 10.01 | -3.59 | -2.87 | -13.55 | 0.96 | 20.32 | 1.25 | 1.62 | -16.81 | 0.47 |
Cytoplasmic Foci | 7.08 | 14.08 | 4.53 | -2.27 | -7.19 | 2.76 | 1.97 | 2.62 | 0.23 | 0.92 | 8.36 | 12.51 | 6.31 | -1.51 | -4.69 |
Eisosomes | 4.09 | 11.28 | -13.18 | -14.53 | -16.58 | 5.95 | -3.23 | 2.27 | -5.27 | 6.12 | 8.17 | 3.54 | -9.87 | -14.80 | -12.12 |
Endoplasmic Reticulum | 10.14 | 16.96 | 12.15 | 1.29 | -4.21 | 3.96 | -0.33 | 5.21 | 0.83 | 5.24 | 12.11 | 13.88 | 14.36 | 1.43 | 0.89 |
Endosome | -0.43 | 9.59 | -5.17 | -4.46 | -8.15 | 1.96 | -4.00 | -2.08 | -4.45 | 2.76 | 1.62 | -0.25 | -5.47 | -6.25 | -4.63 |
Golgi | -2.38 | 5.96 | -2.50 | -1.98 | -2.70 | -0.25 | 0.04 | 0.19 | 0.42 | 0.18 | -1.62 | 1.00 | -2.40 | -1.58 | -2.67 |
Lipid Particles | 20.02* | 22.51 | 20.64 | 0.56 | -11.84 | 5.15 | 8.63 | 10.30 | 4.75 | 2.61 | 19.34 | 23.69 | 22.80 | 4.51 | -4.50 |
Mitochondria | 4.41 | 7.35 | 2.49 | -0.38 | -1.74 | -0.62 | -3.97 | 1.50 | 1.47 | 5.01 | 2.64 | 2.74 | 3.75 | 0.82 | 1.55 |
Mitotic Spindle | -3.68 | -7.06 | -13.39 | -12.20 | -10.88 | -1.29 | -11.62 | -16.29 | -15.78 | -5.15 | -3.82 | -13.35 | -21.26 | -19.92 | -12.57 |
None | -2.04 | 9.98 | 1.11 | 2.57 | -4.86 | 6.56 | 4.35 | 6.02 | -2.02 | 2.42 | 2.87 | 10.16 | 5.53 | 2.06 | -3.42 |
Nuclear Periphery | 4.02 | 7.20 | 26.45* | 19.83* | 17.28 | -4.12 | 6.92 | 3.82 | 8.16 | -3.23 | -1.18 | 9.94 | 17.43 | 18.02 | 7.16 |
Nuclear Periphery Foci | -14.72* | 24.50* | 17.79* | 28.81* | -3.66 | -7.34 | 15.75 | 14.09 | 23.33* | -2.18 | -18.33 | 27.37 | 18.56 | 35.73* | -7.06 |
Nucleolus | -3.24 | 1.25 | -3.40 | -1.07 | -4.17 | 6.13 | -0.27 | 4.54 | -1.91 | 4.44 | 2.21 | 0.57 | -0.18 | -2.02 | -0.66 |
Nucleus | -0.28 | -36.44 | -24.38 | -22.51 | 4.09 | 10.90 | -9.20 | -7.54 | -17.49 | 2.14 | 8.68 | -33.47 | -21.70 | -26.99 | 8.85 |
Peroxisomes | -1.34 | 6.74 | -3.96 | -2.60 | -5.14 | 2.44 | -0.84 | 2.71 | 0.70 | 2.87 | 1.04 | 0.43 | -1.54 | -2.36 | -1.70 |
Vacuole | -3.30 | -4.52 | -15.07 | -13.85 | -14.04 | -0.38 | -14.70 | -10.03 | -9.70 | 8.74 | -2.53 | -14.68 | -17.93 | -16.67 | -4.40 |
Vacuole Periphery | -3.87 | 3.17 | -0.58 | 3.35 | -2.53 | -2.42 | -4.42 | -1.35 | 0.63 | 2.11 | -4.51 | -2.77 | -1.65 | 2.28 | 0.16 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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