Standard name | |
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Human Ortholog | |
Description | Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 1 | – | 4 | 1 | 6 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Bud | 0 | 1 | 12 | 4 | 1 | 4 | – | 19 | 32 | 31 | 22 | 34 | 0 | 1 | 0 | 0 | 0 | 0 | 15 | 10 | 9 | 5 | 5 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 4 | 3 | 4 | 4 | 6 | – | 7 | 15 | 12 | 17 | 23 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 254 | 300 | 252 | 455 | 469 | 488 | – | 434 | 449 | 412 | 340 | 412 | 76 | 199 | 216 | 65 | 110 | 91 | 247 | 284 | 259 | 72 | 152 | 138 |
Endoplasmic Reticulum | 1 | 0 | 0 | 5 | 1 | 2 | – | 7 | 5 | 14 | 23 | 20 | 0 | 0 | 0 | 2 | 0 | 7 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 2 | 1 | 1 | – | 7 | 4 | 4 | 3 | 6 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 5 | 1 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 5 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 |
Mitochondria | 3 | 1 | 3 | 16 | 11 | 70 | – | 79 | 134 | 114 | 111 | 141 | 0 | 0 | 0 | 0 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2 | 0 | 0 | 2 | 0 | 3 | – | 21 | 22 | 20 | 23 | 28 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 2 | 1 | 1 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | – | 16 | 6 | 19 | 11 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 2 | 0 | 0 | – | 11 | 5 | 6 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | – | 5 | 4 | 0 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 0 | 3 |
Vac/Vac Membrane | 4 | 6 | 12 | 17 | 24 | 42 | – | 186 | 126 | 148 | 67 | 91 | 0 | 3 | 10 | 4 | 15 | 8 | 1 | 2 | 1 | 1 | 1 | 1 |
Unique Cell Count | 263 | 304 | 268 | 490 | 492 | 558 | 708 | 682 | 685 | 531 | 683 | 76 | 203 | 228 | 69 | 122 | 99 | 277 | 308 | 286 | 87 | 176 | 169 | |
Labelled Cell Count | 267 | 313 | 283 | 508 | 511 | 619 | 796 | 806 | 792 | 629 | 789 | 76 | 205 | 230 | 71 | 133 | 110 | 277 | 308 | 286 | 87 | 176 | 169 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.2 | 18.9 | 13.0 | 12.9 | 12.1 | 9.5 | – | 12.6 | 17.6 | 19.8 | 22.4 | 25.9 | 16.3 | 18.2 | 21.2 | 61.3 | 48.5 | 51.0 | 21.1 | 19.1 | 18.6 |
Std Deviation (1e-4) | 15.5 | 14.8 | 10.5 | 9.5 | 10.4 | 8.8 | – | 12.1 | 21.0 | 21.3 | 26.5 | 31.1 | 9.5 | 23.1 | 41.8 | 53.2 | 52.6 | 55.4 | 16.2 | 12.4 | 13.4 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.11 | -0.46 | – | -0.05 | 0.43 | 0.6 | 0.78 | 0.99 | 0.32 | 0.48 | 0.7 | 2.23 | 1.89 | 1.97 | 0.69 | 0.55 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -3.4 | 0 | -3.7 | – | -1.4 | 0.1 | 0 | -0.2 | 0.3 | 0 | -2.6 | -3.2 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 1.1 | 0 | 1.8 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 0.8 | -2.9 | – | -10.0 | -8.9 | -10.2 | -9.1 | -10.2 | 2.2 | 2.1 | 0.3 | 0.1 | -1.4 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.5 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.8 | 0 | 5.4 | – | 5.0 | 7.3 | 6.6 | 7.5 | 7.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 2.9 | 3.0 | 2.8 | 3.5 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.7 | 0.2 | 1.7 | – | 7.6 | 5.5 | 6.4 | 3.6 | 3.9 | 0 | -1.8 | 0 | 0 | 2.8 | 1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 147.1001 | 140.8925 | 146.6995 | 185.6852 | 122.0993 | 167.1872 | 69.9479 | 90.5747 | 75.5569 | 87.2527 | 74.8531 | 100.2537 | 63.783 | 80.7772 | 83.0577 | 59.2074 | 66.6158 | 99.707 |
Actin | 0.0042 | 0.0009 | 0.0008 | 0.0002 | 0.0007 | 0.0003 | 0.0072 | 0.0024 | 0.0033 | 0.0013 | 0.001 | 0.0113 | 0.0287 | 0.0045 | 0.0011 | 0.0483 | 0.0006 | 0.0006 |
Bud | 0.0009 | 0.0011 | 0.0039 | 0.0014 | 0.0034 | 0.0003 | 0.0015 | 0.0073 | 0.0007 | 0.0003 | 0.0001 | 0.0006 | 0.0064 | 0.0064 | 0.0022 | 0.0024 | 0.0005 | 0.0002 |
Bud Neck | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0011 | 0.0034 | 0.0002 | 0.0001 | 0.0048 | 0.0002 | 0.0003 |
Bud Periphery | 0.0003 | 0.0002 | 0.0013 | 0.0004 | 0.0008 | 0.0001 | 0.0012 | 0.002 | 0.0006 | 0.0001 | 0 | 0.0003 | 0.0031 | 0.001 | 0.0003 | 0.002 | 0.0001 | 0.0001 |
Bud Site | 0.0006 | 0.0005 | 0.0004 | 0.0001 | 0.001 | 0 | 0.0013 | 0.0033 | 0.0007 | 0.0001 | 0 | 0.0004 | 0.0065 | 0.0028 | 0.0006 | 0.0061 | 0.0001 | 0.0003 |
Cell Periphery | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0.0014 | 0.0002 | 0.0008 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.001 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0 |
Cytoplasm | 0.9241 | 0.9807 | 0.9521 | 0.9742 | 0.861 | 0.9584 | 0.8479 | 0.9595 | 0.9494 | 0.9275 | 0.9752 | 0.9239 | 0.798 | 0.9635 | 0.9502 | 0.8196 | 0.9824 | 0.9455 |
Cytoplasmic Foci | 0.0069 | 0.002 | 0.0018 | 0.0003 | 0.0288 | 0.0046 | 0.018 | 0.0081 | 0.0163 | 0.0125 | 0.0026 | 0.0041 | 0.03 | 0.0021 | 0.0106 | 0.0256 | 0.0055 | 0.0034 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0005 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0.0008 | 0 | 0 |
Endoplasmic Reticulum | 0.0017 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0.006 | 0.0002 | 0.0003 | 0.0069 | 0.0005 | 0.0012 | 0.0058 | 0.0002 | 0.0006 | 0.0038 | 0.0003 | 0.0003 |
Endosome | 0.0046 | 0 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0172 | 0.0029 | 0.0003 | 0.0289 | 0.0003 | 0.0044 | 0.0132 | 0.0009 | 0.0023 | 0.011 | 0.0003 | 0.0283 |
Golgi | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0065 | 0.0011 | 0.0002 | 0.0022 | 0.0001 | 0.007 | 0.0036 | 0.0011 | 0.0005 | 0.0014 | 0.0001 | 0.0095 |
Lipid Particles | 0.0009 | 0.0001 | 0.001 | 0 | 0.0045 | 0.0001 | 0.007 | 0.0003 | 0.0006 | 0.0006 | 0.0002 | 0.0009 | 0.0132 | 0.0015 | 0.0025 | 0.0037 | 0.0001 | 0.0008 |
Mitochondria | 0.0013 | 0.0002 | 0.0026 | 0.0001 | 0.0151 | 0.0003 | 0.0158 | 0.0006 | 0.0044 | 0.0005 | 0.0001 | 0.0017 | 0.0098 | 0.0053 | 0.0019 | 0.006 | 0.0001 | 0.0027 |
None | 0.0064 | 0.0027 | 0.0036 | 0.0008 | 0.023 | 0.0026 | 0.0085 | 0.0046 | 0.0075 | 0.0038 | 0.0019 | 0.0011 | 0.015 | 0.0012 | 0.0021 | 0.036 | 0.0031 | 0.0004 |
Nuclear Periphery | 0.0073 | 0.0017 | 0.0035 | 0.0026 | 0.0027 | 0.0049 | 0.0204 | 0.0012 | 0.0012 | 0.0036 | 0.0036 | 0.0041 | 0.0093 | 0.0011 | 0.0037 | 0.004 | 0.0011 | 0.0008 |
Nucleolus | 0.0072 | 0.0008 | 0.0025 | 0.0001 | 0.0234 | 0.0048 | 0.0021 | 0.0005 | 0.0047 | 0.0003 | 0.0001 | 0.002 | 0.0058 | 0.0001 | 0.0011 | 0.0035 | 0.0001 | 0 |
Nucleus | 0.0242 | 0.0069 | 0.0195 | 0.0183 | 0.0179 | 0.0204 | 0.0223 | 0.0036 | 0.0048 | 0.0075 | 0.0107 | 0.0315 | 0.0263 | 0.0046 | 0.0148 | 0.0084 | 0.0032 | 0.005 |
Peroxisomes | 0.0002 | 0 | 0.0001 | 0 | 0.0013 | 0 | 0.0018 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0079 | 0.002 | 0.0006 | 0.0013 | 0 | 0.0003 |
Punctate Nuclear | 0.0021 | 0.0008 | 0.0007 | 0.0002 | 0.0026 | 0.0004 | 0.0023 | 0.0005 | 0.0012 | 0.0015 | 0.0026 | 0.0024 | 0.0045 | 0.0006 | 0.0028 | 0.0029 | 0.0005 | 0.0003 |
Vacuole | 0.0049 | 0.0008 | 0.0033 | 0.0007 | 0.006 | 0.0014 | 0.0071 | 0.0008 | 0.0017 | 0.0011 | 0.0004 | 0.0011 | 0.005 | 0.0006 | 0.0011 | 0.0066 | 0.0013 | 0.0007 |
Vacuole Periphery | 0.0008 | 0.0001 | 0.0014 | 0.0002 | 0.004 | 0.0003 | 0.0046 | 0.0002 | 0.0008 | 0.0005 | 0.0001 | 0.0003 | 0.0028 | 0.0002 | 0.0006 | 0.0013 | 0.0004 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0309 | 8.9671 | 6.6539 | 6.6087 | 6.5028 | 36.7598 | 22.6665 | 14.2784 | 10.6475 | 7.2884 |
Translational Efficiency | 1.3813 | 1.3448 | 1.9179 | 2.8937 | 2.1972 | 1.4925 | 1.0829 | 2.6065 | 3.1232 | 2.7889 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
131 | 1358 | 1148 | 1502 | 394 | 850 | 2435 | 1177 | 525 | 2208 | 3583 | 2679 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 911.39 | 964.38 | 1074.13 | 3350.86 | 740.17 | 1009.24 | 1614.17 | 2726.70 | 782.89 | 981.65 | 1441.14 | 3076.64 |
Standard Deviation | 368.62 | 449.10 | 428.82 | 4085.99 | 148.41 | 554.18 | 773.75 | 3412.36 | 236.48 | 492.70 | 727.52 | 3817.34 |
Intensity Change Log 2 | — | 0.081533 | 0.237028 | 1.878391 | — | 0.447341 | 1.124864 | 1.881227 | — | 0.257015 | 0.702865 | 1.879663 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000259 | 0.000292 | 0.000309 | 0.003910 | 0.000259 | 0.001773 | 0.000157 | 0.000621 | 0.000259 | 0.000862 | 0.000205 | 0.002465 |
Bud Neck | 0.010222 | 0.011989 | 0.002802 | 0.006399 | 0.010156 | 0.013967 | 0.001923 | 0.004134 | 0.010172 | 0.012751 | 0.002205 | 0.005404 |
Bud Site | 0.013932 | 0.018024 | 0.006148 | 0.044353 | 0.004863 | 0.018226 | 0.007832 | 0.027991 | 0.007126 | 0.018102 | 0.007293 | 0.037165 |
Cell Periphery | 0.000218 | 0.000153 | 0.000184 | 0.006451 | 0.000150 | 0.000640 | 0.000488 | 0.003127 | 0.000167 | 0.000341 | 0.000391 | 0.004991 |
Cytoplasm | 0.630191* | 0.689206* | 0.592023* | 0.648124* | 0.491329* | 0.592488* | 0.829637* | 0.686374* | 0.525978* | 0.651973* | 0.753505* | 0.664929* |
Cytoplasmic Foci | 0.176649 | 0.120945 | 0.011806 | 0.023668 | 0.277131* | 0.170658 | 0.006931 | 0.029683 | 0.252058* | 0.140082 | 0.008493 | 0.026311 |
Eisosomes | 0.000110 | 0.000049 | 0.000028 | 0.000032 | 0.000195 | 0.000113 | 0.000003 | 0.000012 | 0.000174 | 0.000073 | 0.000011 | 0.000023 |
Endoplasmic Reticulum | 0.000985 | 0.000411 | 0.002142 | 0.001213 | 0.000559 | 0.000448 | 0.000088 | 0.001399 | 0.000665 | 0.000425 | 0.000746 | 0.001295 |
Endosome | 0.012416 | 0.012595 | 0.001974 | 0.004368 | 0.013065 | 0.022238 | 0.000139 | 0.010983 | 0.012903 | 0.016307 | 0.000727 | 0.007274 |
Golgi | 0.001711 | 0.003091 | 0.000171 | 0.006781 | 0.000830 | 0.005507 | 0.000117 | 0.008216 | 0.001050 | 0.004021 | 0.000134 | 0.007411 |
Lipid Particles | 0.002556 | 0.001555 | 0.000972 | 0.001360 | 0.006888 | 0.003185 | 0.000183 | 0.001718 | 0.005807 | 0.002182 | 0.000435 | 0.001517 |
Mitochondria | 0.001494 | 0.003919 | 0.000428 | 0.001509 | 0.001969 | 0.004464 | 0.000238 | 0.011163 | 0.001850 | 0.004129 | 0.000299 | 0.005750 |
Mitotic Spindle | 0.003621 | 0.014579 | 0.004950 | 0.047459 | 0.000563 | 0.009389 | 0.012400 | 0.039435 | 0.001326 | 0.012581 | 0.010013 | 0.043934 |
None | 0.001350 | 0.006123 | 0.009020 | 0.014314 | 0.001206 | 0.003909 | 0.012983 | 0.016073 | 0.001242 | 0.005271 | 0.011713 | 0.015087 |
Nuclear Periphery | 0.000262 | 0.000165 | 0.000892 | 0.000437 | 0.000343 | 0.000205 | 0.000161 | 0.000455 | 0.000323 | 0.000181 | 0.000395 | 0.000445 |
Nuclear Periphery Foci | 0.001221 | 0.001116 | 0.002095 | 0.002160 | 0.001121 | 0.001208 | 0.000420 | 0.001799 | 0.001146 | 0.001151 | 0.000957 | 0.002001 |
Nucleolus | 0.000463 | 0.000777 | 0.000318 | 0.000734 | 0.001008 | 0.000649 | 0.000155 | 0.000251 | 0.000872 | 0.000728 | 0.000207 | 0.000522 |
Nucleus | 0.061031 | 0.055812 | 0.304556* | 0.145851* | 0.098217 | 0.058519 | 0.113975 | 0.102714 | 0.088938 | 0.056854 | 0.175038* | 0.126899 |
Peroxisomes | 0.008104 | 0.005192 | 0.000441 | 0.000799 | 0.004779 | 0.005659 | 0.000073 | 0.001473 | 0.005609 | 0.005372 | 0.000191 | 0.001095 |
Vacuole | 0.070596 | 0.052836 | 0.058398 | 0.037269 | 0.084723 | 0.085092 | 0.012020 | 0.046168 | 0.081198 | 0.065253 | 0.026880 | 0.041178 |
Vacuole Periphery | 0.002608 | 0.001173 | 0.000342 | 0.002811 | 0.000648 | 0.001663 | 0.000076 | 0.006210 | 0.001137 | 0.001362 | 0.000161 | 0.004304 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.27 | -1.70 | -1.52 | -1.49 | -1.26 | -1.33 | 0.23 | -0.80 | 1.24 | -2.70 | -1.35 | -0.34 | -1.62 | -0.02 | -1.58 |
Bud Neck | -0.57 | 2.53 | 2.00 | 5.51 | -2.14 | -1.63 | 5.69 | 5.01 | 6.99 | -2.83 | -1.50 | 6.08 | 4.79 | 8.77 | -4.72 |
Bud Site | -1.02 | 2.14 | -1.58 | -0.97 | -6.68 | -5.56 | -2.34 | -6.10 | 1.47 | -4.73 | -6.67 | 0.28 | -6.64 | 0.24 | -8.16 |
Cell Periphery | 1.53 | 0.06 | -4.17 | -4.34 | -4.19 | -1.31 | -1.25 | -4.08 | -1.10 | -3.12 | -1.20 | -1.28 | -5.53 | -4.32 | -4.94 |
Cytoplasm | -2.36 | 4.68 | 0.20 | 6.18 | -10.70 | -6.12 | -20.21 | -12.43 | -6.73 | 11.45 | -9.45 | -12.98 | -9.65 | 0.45 | 6.22 |
Cytoplasmic Foci | 3.71 | 11.17 | 10.54 | 19.91 | -5.34 | 8.91 | 24.83 | 23.00 | 20.46 | -9.64 | 11.70 | 26.62 | 25.03 | 28.16 | -11.04 |
Eisosomes | 4.48 | 5.28 | 6.90 | 8.52 | 6.79 | 2.86 | 7.30 | 7.09 | 5.84 | -7.68 | 4.91 | 7.95 | 8.05 | 8.49 | 1.38 |
Endoplasmic Reticulum | 1.65 | -5.25 | 1.60 | 0.31 | 9.97 | 1.23 | 5.54 | 0.97 | 0.46 | -1.13 | 2.24 | -3.19 | 1.83 | 0.51 | 4.63 |
Endosome | 0.06 | 4.44 | 4.90 | 8.82 | 3.44 | -3.52 | 11.21 | 8.29 | 9.70 | -3.47 | -1.68 | 11.69 | 10.45 | 12.91 | -2.59 |
Golgi | -1.42 | 2.25 | 0.11 | 1.69 | -2.48 | -3.46 | 3.33 | -1.65 | 2.54 | -3.14 | -4.45 | 3.88 | -1.37 | 3.03 | -3.86 |
Lipid Particles | 2.30 | 2.83 | 0.78 | -2.88 | -4.42 | 3.54 | 6.64 | 4.77 | 1.46 | -5.14 | 4.88 | 6.87 | 4.89 | -0.24 | -8.00 |
Mitochondria | -1.71 | 1.18 | 0.99 | 3.09 | -0.89 | -1.42 | 1.88 | -0.22 | 1.20 | -2.08 | -1.92 | 2.12 | 0.59 | 2.87 | -2.36 |
Mitotic Spindle | -2.78 | -0.61 | -5.92 | -3.48 | -7.58 | -4.13 | -8.71 | -8.72 | -3.33 | -3.23 | -5.93 | -7.14 | -11.66 | -4.55 | -7.16 |
None | -8.03 | -9.90 | -10.02 | -2.42 | -0.63 | -4.04 | -16.75 | -10.92 | -5.20 | 1.27 | -9.42 | -19.52 | -15.14 | -5.07 | 1.16 |
Nuclear Periphery | 0.85 | -7.25 | -2.84 | -16.98 | 12.13 | 1.58 | 0.53 | -2.27 | -7.08 | -6.08 | 2.20 | -4.76 | -4.28 | -17.14 | 1.02 |
Nuclear Periphery Foci | 0.41 | -4.26 | -1.32 | -2.10 | 2.70 | -1.02 | 2.33 | -2.79 | -1.83 | -6.13 | -0.38 | -1.41 | -2.77 | -2.78 | -2.08 |
Nucleolus | -1.50 | 1.09 | 0.89 | 3.47 | -0.33 | 1.65 | 3.48 | 3.46 | 7.09 | -0.44 | 0.98 | 3.57 | 3.24 | 5.74 | -1.47 |
Nucleus | 0.53 | -27.27 | -12.11 | -21.36 | 21.36 | 5.39 | -7.48 | -1.69 | -10.53 | 8.68 | 5.54 | -20.05 | -8.76 | -23.20 | 16.14 |
Peroxisomes | 0.54 | 1.43 | 1.45 | 8.49 | 0.58 | -0.47 | 6.37 | 5.94 | 7.25 | -2.88 | 0.31 | 3.83 | 3.73 | 11.13 | -1.85 |
Vacuole | 1.31 | -2.62 | -4.07 | -11.60 | -2.60 | -0.69 | 6.63 | -4.94 | -4.96 | -14.71 | 2.17 | 3.56 | -6.17 | -12.22 | -14.85 |
Vacuole Periphery | 0.94 | 1.47 | 1.19 | 1.10 | -1.53 | -2.55 | 4.07 | -0.83 | 0.39 | -1.65 | -0.29 | 2.41 | 0.38 | 0.85 | -2.33 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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