Standard name
Human Ortholog
Description Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.41 0.25 0.26 0.15 0.06 0.08 0 0 0 0 0 0.35 0.24 0.12 0.23 0.23 0.21 0.24 0.16 0.2 0.19 0.23 0.25
Bud 0.21 0.14 0.24 0.23 0.29 0.17 0.15 0.11 0.13 0.15 0.12 0.2 0.19 0.23 0.12 0.16 0.1 0.3 0.28 0.23 0.25 0.23 0.19
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0.09 0.05 0.05
Bud Site 0 0 0.08 0.08 0.05 0.05 0 0 0 0 0 0.06 0 0 0 0 0
Cell Periphery 0.08 0 0.05 0.06 0 0 0 0 0 0.06 0 0.1 0.09 0.08 0 0 0.1 0 0 0 0 0 0
Cytoplasm 0.26 0.33 0.32 0.38 0.26 0.31 0.38 0.38 0.33 0.27 0.26 0.37 0.53 0.62 0.52 0.52 0.43 0.1 0.14 0.1 0.1 0.08 0.07
Endoplasmic Reticulum 0.11 0.13 0.07 0 0 0.06 0 0 0 0 0 0.08 0.06 0 0.07 0.07 0 0.09 0 0.06 0.07 0.09 0.12
Endosome 0 0 0 0 0.07 0 0 0.05 0 0 0 0 0 0 0.08 0 0 0 0 0 0.05 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0
Mitochondria 0.13 0.24 0.34 0.5 0.43 0.59 0.56 0.56 0.74 0.78 0.79 0.13 0.08 0.26 0.11 0.06 0.1 0.1 0.11 0.2 0.15 0.15 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0.08 0.08 0.1 0 0.1 0.05 0.06 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 100 54 48 40 21 37 17 14 15 9 6 59 62 36 25 41 69 76 48 42 32 67 72
Bud 51 30 44 62 104 80 97 82 53 57 65 34 50 70 13 29 33 96 88 48 43 69 52
Bud Neck 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 2 0 15 21 14 14 15 13
Bud Site 8 7 15 21 19 24 25 32 10 5 14 10 8 4 2 1 4
Cell Periphery 20 7 9 16 7 6 17 13 11 23 23 17 24 25 3 6 32 4 1 2 1 4 9
Cytoplasm 64 71 59 105 94 142 243 290 141 106 140 63 138 192 56 92 142 32 42 21 17 24 20
Endoplasmic Reticulum 28 27 13 9 8 28 18 15 19 7 12 13 15 6 7 12 13 30 10 12 11 27 35
Endosome 9 3 1 1 26 4 20 36 1 1 1 3 5 3 9 5 13 11 10 7 9 14 7
Golgi 9 3 0 0 0 0 0 0 0 0 0 2 3 0 2 10 28 13 5 1 1 5 9
Mitochondria 33 50 63 137 155 269 363 428 311 307 420 22 22 81 12 10 33 32 35 43 26 45 49
Nucleus 0 8 0 0 10 7 6 15 9 6 8 3 2 0 1 2 7 0 1 0 0 1 0
Nuclear Periphery 0 1 0 0 3 0 1 1 0 3 0 0 0 0 0 0 0 1 2 0 0 0 0
Nucleolus 2 3 1 3 7 1 9 10 3 0 5 1 0 2 0 0 5 0 0 0 0 0 0
Peroxisomes 4 6 5 7 24 6 15 16 6 2 7 7 5 6 1 6 6 0 0 1 0 1 3
SpindlePole 3 0 3 1 12 2 22 29 1 1 5 0 2 0 1 2 13 1 4 4 2 3 0
Vac/Vac Membrane 11 8 4 3 13 17 35 50 10 10 11 2 2 2 9 14 32 5 31 10 10 15 8
Unique Cell Count 245 212 185 274 364 459 646 766 423 392 530 171 260 310 107 177 333 324 309 213 174 300 285
Labelled Cell Count 342 278 265 405 503 623 891 1032 590 537 717 236 338 427 141 232 430 324 309 213 174 300 285


Ambiguous, Bud, Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.1 7.5 5.4 5.3 5.2 4.8 4.5 4.7 4.1 3.9 3.6 7.7 7.7 6.6 8.6 9.6 10.2 7.5 7.1 8.2
Std Deviation (1e-4) 1.9 1.5 0.9 1.1 1.3 0.9 1.0 1.4 1.3 0.8 1.1 1.5 1.8 1.6 2.1 2.3 2.4 1.2 1.3 1.2
Intensity Change (Log2) -0.02 -0.04 -0.17 -0.26 -0.19 -0.37 -0.47 -0.57 0.52 0.51 0.3 0.69 0.84 0.93 0.48 0.41 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.0 -6.7 0 0 0 0 0 0 1.8 -0.5 -4.1 -0.5 -0.6 -1.4
Bud -0.3 1.2 0 0 0 0 0 0 -0.9 -1.2 -0.3 -2.4 -1.8 -4.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -0.2 -1.3 0 0 0 0 0 0 -0.8 -2.4 -3.8 -2.2 -3.5 -4.0
Cell Periphery 0.5 -1.9 0 0 0 0 0 0 1.8 1.7 1.4 0 -0.7 1.9
Cytoplasm 1.4 -1.5 -0.2 1.4 1.5 0.3 -1.2 -1.4 1.0 4.4 6.5 3.4 3.9 2.4
Endoplasmic Reticulum -1.8 -2.8 0 0 0 0 0 0 0.2 -0.5 -2.9 -0.2 -0.1 -1.6
Endosome 0 3.4 0 0 0 0 0 0 0 0 0 0 0 2.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 4.1
Mitochondria 3.4 1.9 0 0 0 0 0 0 -4.7 -6.8 -1.9 -4.3 -6.7 -6.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1.9 0 0 0 0 0 0 0.7 0 0 0 0.4 0
SpindlePole 0 1.1 0 0 0 0 0 0 0 0 0 0 0 1.4
Vacuole 0 0.9 1.0 1.8 2.3 0 0 0 0 0 0 0 2.5 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8468 5.3492 5.016 4.6237 4.7284 5.3108 5.0765 4.9959 5.0275 5.1842 4.791 5.1915 2.3857 2.4533 2.8398 2.9148 3.2668 2.7155
Actin 0.1218 0.0103 0.0284 0.0184 0.0703 0.0637 0.1147 0.0261 0.036 0.0365 0.1407 0.0684 0.0516 0.0478 0.0414 0.0338 0.0977 0.0481
Bud 0.0577 0.0404 0.3146 0.1643 0.1252 0.0153 0.046 0.0514 0.3451 0.209 0.159 0.0214 0.0255 0.0644 0.2622 0.1105 0.1572 0.0245
Bud Neck 0.0203 0.0221 0.0086 0.026 0.0266 0.4519 0.066 0.0099 0.0043 0.0515 0.054 0.4572 0.026 0.0053 0.0218 0.0038 0.0012 0.2739
Bud Periphery 0.1332 0.0571 0.4654 0.5973 0.4256 0.0503 0.05 0.0351 0.3732 0.5385 0.3193 0.0436 0.0879 0.0496 0.3909 0.421 0.4123 0.0899
Bud Site 0.1485 0.5272 0.1154 0.0254 0.0543 0.037 0.1465 0.4675 0.0708 0.0237 0.0135 0.0202 0.1087 0.389 0.1235 0.0192 0.0112 0.017
Cell Periphery 0.0234 0.0086 0.0041 0.0061 0.0045 0.0183 0.0053 0.005 0.0027 0.003 0.0021 0.0026 0.0135 0.0047 0.004 0.0026 0.0023 0.0114
Cytoplasm 0.1857 0.2314 0.0347 0.0639 0.0846 0.1643 0.2317 0.3327 0.095 0.0604 0.1306 0.1923 0.4084 0.2663 0.0643 0.2323 0.106 0.3403
Cytoplasmic Foci 0.0522 0.0256 0.0025 0.0119 0.04 0.0503 0.0717 0.0144 0.0112 0.004 0.0322 0.0512 0.0512 0.0404 0.0124 0.0268 0.0967 0.0521
Eisosomes 0.0019 0.0002 0.0002 0.0001 0.0003 0.0008 0.0004 0.0002 0.0001 0.0005 0.0002 0.0001 0.0009 0.0006 0.0002 0.0003 0.0003 0.0004
Endoplasmic Reticulum 0.0373 0.0017 0.001 0.0027 0.0028 0.0077 0.02 0.0055 0.0016 0.0011 0.0118 0.0043 0.0287 0.0032 0.0013 0.0036 0.0021 0.0088
Endosome 0.053 0.0164 0.0027 0.0128 0.0436 0.0472 0.0576 0.0108 0.0094 0.004 0.0233 0.0387 0.0213 0.0204 0.0264 0.0452 0.0038 0.0257
Golgi 0.0374 0.0106 0.0017 0.0069 0.0199 0.0326 0.0571 0.0041 0.0102 0.0031 0.0262 0.0359 0.0182 0.009 0.0219 0.0123 0.0031 0.016
Lipid Particles 0.022 0.0007 0.0018 0.011 0.0194 0.0026 0.0171 0.0006 0.007 0.0008 0.018 0.0086 0.014 0.0023 0.0016 0.0019 0.0015 0.0019
Mitochondria 0.0333 0.0041 0.002 0.0118 0.0154 0.0226 0.0245 0.0022 0.0107 0.0425 0.0295 0.013 0.0031 0.0119 0.016 0.022 0.0036 0.0129
None 0.013 0.0179 0.004 0.0035 0.0073 0.0024 0.0309 0.0225 0.0073 0.0014 0.0032 0.0242 0.1248 0.0685 0.0022 0.0442 0.0939 0.0657
Nuclear Periphery 0.0052 0.0008 0.0005 0.0015 0.0022 0.0006 0.0051 0.0009 0.0007 0.001 0.0051 0.0006 0.0019 0.0011 0.0004 0.0014 0.0004 0.0007
Nucleolus 0.0021 0.0019 0.0002 0.0008 0.0067 0.0006 0.0016 0.0004 0.0004 0.0002 0.0002 0.0003 0.0005 0.0007 0.0002 0.0004 0.0001 0.0004
Nucleus 0.0042 0.0046 0.0011 0.0021 0.0052 0.0016 0.0034 0.0017 0.0016 0.0014 0.0017 0.0012 0.0013 0.0022 0.0008 0.0018 0.0005 0.0009
Peroxisomes 0.014 0.0015 0.0031 0.0076 0.0075 0.0086 0.0177 0.0008 0.0058 0.0007 0.01 0.0072 0.0033 0.0019 0.0026 0.0019 0.0041 0.0041
Punctate Nuclear 0.0053 0.0013 0.0002 0.0008 0.0039 0.0006 0.0178 0.0007 0.0007 0.0002 0.0008 0.0015 0.001 0.0013 0.0002 0.0004 0.0006 0.0006
Vacuole 0.0242 0.0144 0.0068 0.021 0.0293 0.019 0.0123 0.0067 0.005 0.0133 0.012 0.0055 0.0076 0.008 0.0052 0.0117 0.0012 0.0037
Vacuole Periphery 0.0042 0.0012 0.0009 0.0042 0.0053 0.0022 0.0026 0.0007 0.0012 0.0031 0.0067 0.0018 0.0008 0.0012 0.0008 0.0028 0.0003 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.6058 29.9308 42.5967 39.9465 40.8191 26.7208 37.6029 44.1282 34.2137 44.7939
Translational Efficiency 0.3935 0.4127 0.3371 0.2799 0.2394 0.4558 0.3217 0.2204 0.2635 0.2372

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
845 1360 1558 540 1291 1956 1689 70 2136 3316 3247 610

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 776.33 938.84 1157.30 996.50 804.18 879.47 1076.39 1024.92 793.16 903.82 1115.21 999.76
Standard Deviation 141.28 153.89 169.73 146.46 125.69 134.30 172.87 175.97 132.78 145.62 176.07 150.42
Intensity Change Log 2 0.274209 0.576021 0.360200 0.129116 0.420611 0.349921 0.202208 0.499039 0.354979

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006717 0.005706 0.014447 0.003938 0.003674 0.004794 0.005439 0.013142 0.004878 0.005168 0.009761 0.004995
Bud Neck 0.047246 0.015807 0.020239 0.010551 0.031986 0.024886 0.021091 0.020977 0.038023 0.021163 0.020682 0.011748
Bud Site 0.454420* 0.447820* 0.347351* 0.397359* 0.439903* 0.454787* 0.479804* 0.404993* 0.445646* 0.451930* 0.416250* 0.398235*
Cell Periphery 0.005472 0.001525 0.000701 0.000363 0.003258 0.001363 0.000375 0.000866 0.004134 0.001429 0.000531 0.000421
Cytoplasm 0.185885* 0.282155* 0.395589* 0.384283* 0.260623* 0.294939* 0.353701* 0.347958* 0.231057* 0.289696* 0.373800* 0.380114*
Cytoplasmic Foci 0.112267 0.112957 0.075934 0.083786 0.121432 0.092889 0.041674 0.106397 0.117806 0.101119 0.058113 0.086381
Eisosomes 0.000647 0.000155 0.000082 0.000033 0.000327 0.000280 0.000039 0.000080 0.000454 0.000229 0.000059 0.000039
Endoplasmic Reticulum 0.008253 0.006359 0.004969 0.001008 0.005528 0.002639 0.001148 0.000590 0.006606 0.004165 0.002982 0.000960
Endosome 0.028133 0.024291 0.011424 0.014340 0.024347 0.021764 0.007710 0.004880 0.025845 0.022800 0.009492 0.013254
Golgi 0.039047 0.032753 0.005602 0.005257 0.030827 0.030779 0.003076 0.002418 0.034079 0.031588 0.004288 0.004931
Lipid Particles 0.003277 0.001110 0.004080 0.000987 0.003008 0.000894 0.001215 0.001213 0.003114 0.000983 0.002590 0.001013
Mitochondria 0.047108 0.018091 0.000835 0.001560 0.031368 0.027204 0.001110 0.000083 0.037595 0.023466 0.000978 0.001391
Mitotic Spindle 0.003404 0.004733 0.020054 0.009313 0.001447 0.002952 0.015671 0.002359 0.002221 0.003683 0.017774 0.008515
None 0.004472 0.001228 0.000388 0.000740 0.000747 0.000250 0.000261 0.000054 0.002221 0.000651 0.000322 0.000661
Nuclear Periphery 0.000138 0.000024 0.000248 0.000074 0.000040 0.000023 0.000096 0.000036 0.000079 0.000024 0.000169 0.000069
Nuclear Periphery Foci 0.000873 0.000159 0.001992 0.000565 0.000207 0.000074 0.000718 0.001134 0.000471 0.000108 0.001329 0.000630
Nucleolus 0.000526 0.000113 0.000103 0.000059 0.000338 0.000066 0.000224 0.000046 0.000412 0.000085 0.000166 0.000057
Nucleus 0.006688 0.002542 0.019378 0.002476 0.002225 0.001139 0.005717 0.004420 0.003991 0.001714 0.012272 0.002699
Peroxisomes 0.006787 0.004260 0.005012 0.006927 0.003774 0.004302 0.006073 0.013258 0.004966 0.004285 0.005564 0.007654
Vacuole 0.019558 0.023583 0.070083 0.072073 0.020009 0.022235 0.053389 0.074742 0.019831 0.022788 0.061399 0.072379
Vacuole Periphery 0.019082 0.014629 0.001490 0.004307 0.014933 0.011741 0.001469 0.000355 0.016574 0.012925 0.001479 0.003853

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.65 -3.54 2.02 1.70 6.18 -1.35 -1.67 -1.74 -1.54 -1.50 -0.37 -4.39 0.12 0.38 3.42
Bud Neck 8.00 7.27 9.74 4.04 5.63 2.70 5.13 2.19 1.04 0.07 7.57 8.82 12.21 7.30 6.08
Bud Site 0.45 10.83 4.76 4.81 -3.78 -1.22 1.21 1.85 2.21 1.48 -0.66 8.49 5.17 5.80 0.04
Cell Periphery 6.46 8.09 8.61 7.59 4.95 3.40 5.31 3.90 1.66 -1.53 6.51 8.94 9.12 9.81 1.68
Cytoplasm -9.14 -19.70 -13.84 -7.52 0.25 -3.67 -10.76 -2.75 -1.75 0.58 -8.28 -20.29 -11.96 -7.70 -0.34
Cytoplasmic Foci -0.09 6.25 3.24 3.63 -1.85 4.96 14.83 0.45 -1.08 -3.80 3.75 14.81 4.25 1.93 -5.10
Eisosomes 4.78 5.57 6.05 7.46 8.14 1.12 11.16 6.45 4.54 -1.59 4.69 9.12 9.57 8.99 4.17
Endoplasmic Reticulum 1.08 2.16 5.03 4.89 6.09 3.09 4.85 5.52 4.26 1.53 2.71 4.39 6.94 5.57 4.80
Endosome 1.66 8.53 5.60 5.01 -1.49 1.43 10.66 10.42 10.75 1.86 2.14 13.44 6.82 5.84 -2.14
Golgi 1.79 11.70 11.21 12.96 -0.06 0.02 14.03 14.03 15.55 0.77 1.18 18.12 15.93 17.44 -0.81
Lipid Particles 4.42 -2.36 4.46 0.13 9.59 3.70 2.50 0.87 -1.79 -0.95 5.38 0.36 4.45 -1.02 6.36
Mitochondria 6.69 11.50 11.26 9.87 -1.08 1.50 13.86 14.35 15.60 4.77 5.87 17.67 17.09 16.96 -0.65
Mitotic Spindle -1.06 -8.63 -2.43 -1.94 3.78 -2.89 -8.58 -1.19 0.37 7.03 -2.47 -12.46 -3.03 -2.36 3.88
None 2.22 3.15 2.80 0.66 -0.57 3.17 2.99 4.51 4.56 6.81 2.66 3.61 2.51 0.08 -0.66
Nuclear Periphery 2.49 -5.00 0.94 -2.49 7.60 1.45 -6.07 -1.03 -2.32 4.41 2.85 -7.54 -0.22 -2.69 6.64
Nuclear Periphery Foci 1.57 -3.56 0.55 -3.81 8.04 1.99 -5.08 -1.65 -1.75 -1.13 1.97 -5.80 -1.37 -3.77 4.05
Nucleolus 3.34 3.19 3.74 1.92 4.24 1.89 0.28 2.04 0.74 1.72 3.28 1.64 3.50 1.02 2.03
Nucleus 1.98 -9.20 1.79 -0.78 15.30 2.83 -8.54 -2.62 -3.61 3.23 2.62 -12.80 0.53 -3.98 16.25
Peroxisomes 3.57 2.98 0.29 -2.36 -1.98 -1.38 -3.08 -2.19 -2.04 -1.70 1.87 -0.21 -2.30 -3.10 -2.16
Vacuole -1.77 -19.50 -10.17 -9.58 1.81 -1.12 -14.96 -4.27 -4.16 -1.06 -1.99 -24.26 -11.08 -10.67 0.05
Vacuole Periphery 1.84 8.36 6.33 6.65 -2.20 2.41 12.34 13.78 14.11 2.93 2.92 14.26 9.00 7.83 -2.06