Standard name
Human Ortholog
Description Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.87 0.96 0.98 0.96 0.96 0.84 0.73 0.73 0.64 0.62 0.54 0.66 0.99 1.0 0.99 0.94 0.97 0.96 0.84 0.86 0.88 0.98 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.05 0 0.08 0.11 0.4 0.45 0.44 0.46 0.52 0.61 0.5 0 0 0 0 0 0 0.05 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.13 0.08 0.1 0.07 0 0 0 0 0 0 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 2
Bud 1 1 3 1 2 7 9 6 12 13 15 9 0 0 0 0 0 0 1 0 0 0 1 8
Bud Neck 0 0 2 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 0 1 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 1 4 4 1 7 3 5 15 3 3 3 4 10 9 0 0 1 0 0 0
Cytoplasm 98 128 257 101 228 298 306 284 315 268 245 370 276 384 455 119 202 168 102 119 234 299 374 361
Endoplasmic Reticulum 0 1 1 0 0 0 2 0 0 3 0 1 1 0 2 5 3 5 0 0 0 0 0 0
Endosome 0 0 3 0 0 1 0 2 1 0 1 1 1 0 0 0 1 1 4 1 2 0 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 0 0 1 0 3 2
Mitochondria 25 6 4 8 26 142 189 170 229 225 275 280 1 0 1 1 5 0 6 2 17 1 4 11
Nucleus 0 1 0 1 1 0 4 4 6 5 8 10 0 0 1 4 0 1 0 0 0 0 2 3
Nuclear Periphery 1 0 0 0 0 5 1 0 2 4 1 13 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 3 0 1 0 0 0 0 0 0 0 1 0 1 0 1 4
Vac/Vac Membrane 1 1 1 0 3 3 13 18 66 34 47 37 0 0 1 5 4 2 0 11 0 0 1 3
Unique Cell Count 113 133 262 105 237 356 417 388 496 429 451 564 279 384 459 126 209 175 122 139 268 304 403 407
Labelled Cell Count 127 139 272 111 261 461 532 487 641 556 601 737 284 387 463 140 227 187 122 139 268 304 403 407


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.6 4.0 3.7 4.5 4.0 4.1 4.0 4.1 4.0 3.9 4.2 5.6 5.7 5.8 8.1 7.9 8.4 5.6 5.8 6.0
Std Deviation (1e-4) 0.7 1.0 0.8 0.7 2.0 1.9 1.7 1.5 1.4 1.2 1.4 1.3 1.1 0.9 1.0 1.4 1.3 2.0 1.1 1.7 1.3
Intensity Change (Log2) – – – -0.14 0.15 -0.02 0.02 0.0 0.04 0.01 -0.03 0.05 0.48 0.5 0.53 1.0 0.98 1.06 0.47 0.53 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1386 1.8994 1.672 2.0684 1.3288 1.6628 0.8679 0.9665 0.7863 0.1832 0.658 0.8026 -0.0874 0.0901 -0.1092 -0.2864 0.4193 -0.0748
Actin 0.0029 0.001 0.0015 0.0004 0.0035 0.0018 0.0461 0.0029 0.0097 0.0001 0.0068 0.0033 0.027 0.0015 0.018 0.0082 0.0734 0.0051
Bud 0.0001 0.0001 0.0001 0.0002 0.0009 0.0001 0.0017 0.0032 0.0007 0 0.0009 0.0004 0.0013 0.0011 0.0039 0.0003 0.001 0.0004
Bud Neck 0.0004 0.0001 0.0002 0.0001 0.0004 0.0005 0.0034 0.0002 0.003 0.0001 0.0005 0.0014 0.0012 0.0003 0.0014 0.0002 0.0018 0.0024
Bud Periphery 0.0002 0 0.0001 0.0001 0.0007 0.0001 0.0025 0.0041 0.001 0 0.0024 0.0004 0.0009 0.0007 0.0028 0.0006 0.0028 0.0005
Bud Site 0.0017 0.0008 0.0004 0.0001 0.0001 0.0001 0.011 0.0146 0.0026 0 0.0011 0.0002 0.0077 0.0103 0.0073 0.0007 0.0027 0.001
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0.0001 0 0.0004 0.0006 0.0003 0 0.0002 0 0.0003 0.0001 0.0002 0.0001 0.0004 0.0001
Cytoplasm 0.4525 0.5867 0.479 0.5695 0.49 0.5607 0.3795 0.5454 0.4864 0.5349 0.5425 0.6568 0.413 0.5291 0.5352 0.4184 0.4247 0.6039
Cytoplasmic Foci 0.0057 0.0037 0.0086 0.0038 0.0048 0.0077 0.054 0.0357 0.069 0.0046 0.0452 0.0423 0.0192 0.008 0.0143 0.0079 0.0165 0.0137
Eisosomes 0.0003 0.0001 0.0004 0 0.0001 0 0.0004 0.0001 0.0002 0 0.0002 0 0.0004 0.0002 0.0001 0.0004 0.0013 0.0001
Endoplasmic Reticulum 0.0061 0.0026 0.0021 0.0019 0.0016 0.0011 0.0066 0.0025 0.0024 0.0027 0.0018 0.0019 0.0034 0.0033 0.003 0.0019 0.0036 0.0017
Endosome 0.0044 0.0007 0.0057 0.0015 0.0018 0.0016 0.0381 0.0078 0.0128 0.0019 0.0095 0.0072 0.0143 0.003 0.009 0.0068 0.0271 0.0038
Golgi 0.0019 0.0002 0.0006 0.0001 0.0002 0.0002 0.009 0.002 0.004 0.0001 0.0053 0.0009 0.0044 0.0004 0.0022 0.0014 0.0177 0.0012
Lipid Particles 0.0049 0.0001 0.0029 0 0.0001 0.0002 0.0173 0.0067 0.006 0.0001 0.0063 0.0025 0.0131 0.0004 0.0087 0.0074 0.0364 0.0015
Mitochondria 0.0046 0.0001 0.0036 0.0002 0.0003 0.0001 0.0137 0.009 0.0037 0.0002 0.0566 0.0002 0.0057 0.0003 0.0017 0.0034 0.009 0.0008
None 0.5082 0.4013 0.4896 0.4187 0.4924 0.4228 0.3513 0.3593 0.3629 0.4507 0.3 0.2707 0.4512 0.4338 0.3693 0.5207 0.3004 0.3455
Nuclear Periphery 0.0011 0.0005 0.0008 0.0006 0.0007 0.0003 0.0094 0.0006 0.0023 0.0011 0.001 0.0014 0.0067 0.0012 0.0043 0.004 0.0224 0.0024
Nucleolus 0.0003 0.0001 0.0001 0 0 0 0.001 0.0001 0.0002 0 0.0006 0.0001 0.0012 0.0002 0.0006 0.0014 0.0016 0.0002
Nucleus 0.001 0.0008 0.0011 0.0015 0.0011 0.0008 0.006 0.0017 0.0022 0.0019 0.0018 0.002 0.0066 0.0032 0.0041 0.0032 0.003 0.0038
Peroxisomes 0.0016 0.0001 0.0009 0.0001 0.0001 0.0002 0.0282 0.0013 0.0196 0.0001 0.0054 0.0015 0.0139 0.0002 0.0049 0.0058 0.0267 0.0026
Punctate Nuclear 0.0006 0.0005 0.0009 0.0003 0.0004 0.0012 0.0146 0.0008 0.0097 0.0006 0.007 0.0061 0.0059 0.0015 0.0073 0.0058 0.024 0.0083
Vacuole 0.0008 0.0004 0.0009 0.0005 0.0006 0.0003 0.0041 0.0011 0.0011 0.0006 0.0031 0.0007 0.0018 0.0009 0.0015 0.001 0.0016 0.0006
Vacuole Periphery 0.0002 0 0.0002 0.0001 0.0001 0 0.0018 0.0002 0.0003 0.0001 0.0018 0.0002 0.0009 0.0001 0.0004 0.0006 0.0019 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.988 6.62 12.0079 20.9573 18.136 18.0921 16.1758 17.8195 20.8305 18.373
Translational Efficiency 0.9495 1.1811 0.9544 0.9791 0.9117 0.9755 0.6024 0.9331 1.2954 0.9123

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
83 163 94 1040 1965 1564 283 473 2048 1727 377 1513

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 962.40 773.34 938.54 927.50 696.34 720.95 977.78 945.15 707.12 725.89 968.00 933.02
Standard Deviation 146.94 116.57 92.79 145.81 77.89 85.49 117.78 153.81 97.20 90.20 113.35 148.58
Intensity Change Log 2 — -0.315534 -0.036218 -0.053289 — 0.050107 0.489718 0.440751 — -0.150628 0.208251 0.174994

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001409 0.000540 0.000498 0.000410 0.000085 0.000329 0.000274 0.000867 0.000138 0.000349 0.000330 0.000553
Bud Neck 0.015475 0.028272 0.001566 0.004017 0.009567 0.032887 0.001671 0.006863 0.009806 0.032452 0.001645 0.004907
Bud Site 0.095055 0.026718 0.015743 0.029350 0.003029 0.013669 0.011272 0.029807 0.006759 0.014901 0.012387 0.029493
Cell Periphery 0.000561 0.000152 0.000271 0.000105 0.000104 0.000134 0.000183 0.000141 0.000122 0.000136 0.000205 0.000116
Cytoplasm 0.473833* 0.345734* 0.362997* 0.512285* 0.550397* 0.405941* 0.399713* 0.465074* 0.547294* 0.400259* 0.390558* 0.497525*
Cytoplasmic Foci 0.185307* 0.275576* 0.044165 0.022619 0.174279 0.235641* 0.025043 0.047549 0.174726 0.239410* 0.029811 0.030412
Eisosomes 0.000081 0.000140 0.000127 0.000036 0.000112 0.000155 0.000091 0.000053 0.000110 0.000154 0.000100 0.000041
Endoplasmic Reticulum 0.008856 0.001334 0.004375 0.001881 0.000776 0.000468 0.003890 0.001125 0.001104 0.000550 0.004011 0.001644
Endosome 0.022262 0.040329 0.007393 0.009771 0.004746 0.029367 0.004315 0.028842 0.005456 0.030402 0.005083 0.015733
Golgi 0.005140 0.007705 0.000326 0.012820 0.001163 0.004954 0.000087 0.009409 0.001324 0.005214 0.000147 0.011754
Lipid Particles 0.010015 0.009477 0.005520 0.000990 0.005442 0.008942 0.002652 0.003447 0.005627 0.008992 0.003367 0.001758
Mitochondria 0.002111 0.005751 0.000647 0.003116 0.004808 0.011033 0.000393 0.020499 0.004698 0.010535 0.000456 0.008550
Mitotic Spindle 0.008058 0.003467 0.008336 0.088484 0.001183 0.009582 0.000436 0.067456 0.001462 0.009005 0.002405 0.081910
None 0.002688 0.002621 0.002185 0.009089 0.002168 0.002118 0.003797 0.003335 0.002189 0.002166 0.003395 0.007290
Nuclear Periphery 0.000645 0.004383 0.001613 0.001063 0.000446 0.000480 0.001631 0.000855 0.000454 0.000849 0.001626 0.000998
Nuclear Periphery Foci 0.007823 0.001327 0.003630 0.003320 0.000243 0.000312 0.002404 0.001766 0.000551 0.000408 0.002710 0.002834
Nucleolus 0.002230 0.002168 0.000832 0.000595 0.000855 0.001865 0.000365 0.000464 0.000911 0.001893 0.000482 0.000554
Nucleus 0.067604 0.105064 0.410798* 0.101786 0.203680* 0.136330* 0.391235* 0.065816 0.198165* 0.133379* 0.396113* 0.090541
Peroxisomes 0.006455 0.024596 0.005375 0.000507 0.002890 0.013240 0.000344 0.006014 0.003034 0.014312 0.001599 0.002229
Vacuole 0.077305 0.111935 0.123275 0.189650 0.033361 0.090168 0.150036 0.229283* 0.035142 0.092223 0.143363 0.202040*
Vacuole Periphery 0.007088 0.002710 0.000325 0.008108 0.000667 0.002384 0.000170 0.011335 0.000927 0.002414 0.000208 0.009117

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.03 2.75 4.02 3.01 2.14 -4.27 -7.62 -3.63 -1.35 -1.04 -3.92 -6.43 -5.17 -0.29 1.01
Bud Neck -1.75 2.37 2.08 5.09 -2.85 -10.38 8.27 5.33 13.07 -1.21 -10.70 8.85 7.21 14.53 -2.71
Bud Site 2.98 3.59 3.63 2.03 -0.32 -6.73 -1.00 -4.68 -0.77 -2.49 -4.53 -0.24 -4.07 0.30 -2.19
Cell Periphery 2.10 1.43 2.47 4.20 6.10 -3.68 -5.39 1.84 4.53 6.00 -1.21 -5.00 4.15 6.94 9.07
Cytoplasm 3.11 3.87 1.34 -3.39 -4.29 16.57 17.42 10.46 0.75 -5.25 17.38 18.81 14.75 -1.19 -8.23
Cytoplasmic Foci -3.57 7.02 8.28 15.45 3.34 -9.24 35.59 30.49 35.96 -3.65 -10.09 34.32 37.28 43.14 0.89
Eisosomes -3.21 -3.31 3.64 7.21 7.20 -5.59 0.80 11.33 14.74 7.40 -5.92 -0.57 14.46 17.78 11.12
Endoplasmic Reticulum 3.04 0.93 2.94 -1.13 5.93 1.78 -10.34 -1.56 -6.40 10.18 2.76 -11.11 -1.77 -9.37 11.09
Endosome -2.37 3.19 4.10 6.68 3.69 -14.06 1.30 -3.03 7.21 -3.31 -14.80 0.94 -0.57 13.67 -1.15
Golgi -0.66 3.06 0.89 1.25 -2.25 -6.39 4.19 -2.08 1.83 -3.39 -6.31 4.63 -1.88 1.94 -3.26
Lipid Particles 0.15 1.27 2.53 2.92 5.23 -3.70 6.04 6.36 7.56 1.89 -3.66 4.67 9.97 9.24 6.59
Mitochondria -2.26 2.41 1.55 3.10 -1.58 -3.68 4.73 -0.67 1.42 -2.41 -3.70 4.74 1.52 5.04 -2.86
Mitotic Spindle 1.14 0.52 -5.47 -7.96 -6.15 -4.90 1.05 -5.12 -3.54 -5.23 -4.80 -0.38 -9.87 -7.34 -9.57
None -0.03 0.46 -1.38 -1.67 -4.06 -0.16 -1.37 -0.76 -0.54 0.42 -0.22 -0.95 -3.57 -3.15 -2.90
Nuclear Periphery -0.99 -4.66 -4.27 0.65 1.45 -0.45 -12.62 -4.47 -5.43 9.51 -1.10 -13.98 -10.66 -2.21 6.60
Nuclear Periphery Foci 3.49 1.88 3.15 -1.15 2.57 -1.85 -9.27 -7.99 -7.26 4.84 1.28 -9.12 -4.33 -4.90 3.93
Nucleolus -0.07 1.59 1.97 3.50 1.63 -3.32 2.28 2.48 7.00 1.09 -3.40 2.07 2.08 6.57 0.36
Nucleus -2.35 -11.67 -5.17 -1.78 10.20 11.33 -12.54 19.17 8.23 19.94 11.30 -14.93 15.23 3.03 20.89
Peroxisomes -4.30 1.11 3.63 6.54 1.38 -9.58 7.55 0.72 8.53 -2.59 -10.69 3.00 5.13 13.09 0.25
Vacuole -1.52 -3.43 -13.97 -14.45 -6.37 -16.09 -14.85 -25.65 -21.18 -7.15 -16.46 -16.00 -42.32 -32.53 -8.47
Vacuole Periphery 1.05 1.61 1.15 0.48 -2.22 -5.99 5.77 -2.55 -1.11 -3.00 -4.77 3.98 -2.44 -0.27 -3.65
Standard name
Human Ortholog
Description Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (44%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cmk1

Cmk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cmk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available