Standard name
Human Ortholog
Description Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.99 0.99 0.94 0.85 – 0.73 0.74 0.69 0.69 1.0 0.99 0.99 0.97 0.96 0.97 0.94 0.94 0.95 0.9 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.06 – 0.18 0.15 0.25 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.12 – 0.2 0.14 0.11 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 – 0 2 3 3 0 0 0 0 0 0 1 0 0 1 3 1
Bud 0 1 0 1 0 6 1 – 5 7 9 12 0 0 0 0 1 0 2 1 0 3 7 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 3 4 2 11 – 12 17 7 25 0 4 7 5 4 2 0 0 0 0 0 0
Cytoplasm 301 122 158 271 361 282 431 – 334 326 257 343 295 396 494 194 172 120 290 121 145 206 417 357
Endoplasmic Reticulum 2 0 1 0 0 0 1 – 4 3 4 7 0 2 0 2 3 1 0 0 1 0 4 3
Endosome 0 0 1 0 0 0 4 – 2 1 1 2 0 0 0 0 0 0 5 1 1 1 3 1
Golgi 2 0 0 1 1 0 0 – 0 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0
Mitochondria 2 5 0 5 6 31 28 – 82 67 92 112 0 1 0 0 0 2 2 1 0 1 3 2
Nucleus 0 1 0 0 0 3 4 – 5 4 6 8 0 0 2 2 3 0 0 0 0 0 1 1
Nuclear Periphery 1 0 0 0 0 1 1 – 3 2 0 6 0 0 0 1 0 0 0 0 0 1 0 2
Nucleolus 0 0 0 0 0 0 2 – 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 3
Vac/Vac Membrane 2 0 4 0 6 4 61 – 93 62 42 62 0 0 1 9 7 3 3 0 0 2 5 1
Unique Cell Count 310 125 161 273 366 299 508 460 438 375 500 295 398 497 201 179 124 311 129 153 229 458 386
Labelled Cell Count 310 129 164 282 378 330 545 541 491 423 580 295 403 504 213 191 129 311 129 153 229 458 386


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.3 6.2 5.9 6.8 5.0 6.7 – 6.6 7.1 6.7 7.8 6.6 7.3 8.8 10.2 10.1 9.6 5.5 5.7 6.1
Std Deviation (1e-4) 0.8 0.9 1.5 1.6 1.7 1.0 1.3 – 1.8 1.8 1.8 15.1 1.8 1.9 1.9 2.2 1.9 1.8 1.9 2.5 5.0
Intensity Change (Log2) – – – -0.08 0.14 -0.31 0.11 – 0.09 0.19 0.11 0.33 0.09 0.24 0.51 0.72 0.71 0.63 -0.18 -0.11 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 2.9 0 0 0 0 0 0
Cytoplasm 1.1 0.4 -1.9 -4.5 – -6.9 -6.5 -7.5 -7.6 2.4 1.5 1.5 -0.9 -1.1 -0.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 3.0 – 5.8 5.3 6.9 6.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.6 – 5.3 4.0 3.3 3.6 0 0 0 1.0 0.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0015 2.7294 2.732 2.359 1.4511 2.3515 3.6878 5.0734 4.4619 3.9511 3.9733 4.4521 5.1365 6.7844 5.2914 5.5083 5.4584 5.8071
Actin 0.0144 0.0046 0.032 0.0608 0.0079 0.011 0.0333 0.0003 0.0115 0.0002 0.0001 0.0015 0.026 0.0005 0.0061 0.0002 0.0277 0.0076
Bud 0.0004 0.0002 0.0005 0.0003 0.0004 0.0003 0.0006 0.0005 0.0002 0.0002 0.0001 0.0001 0.0009 0.0001 0.0009 0.0001 0.0001 0.0001
Bud Neck 0.0015 0.0002 0.001 0.0005 0.0005 0.0014 0.0016 0.0001 0.0003 0.0003 0.0003 0.0008 0.0021 0.0001 0.0005 0.0003 0.0006 0.0008
Bud Periphery 0.0006 0.0001 0.0005 0.0003 0.0004 0.0003 0.0008 0.0001 0.0002 0 0 0 0.0021 0 0.0007 0 0.0001 0.0001
Bud Site 0.0035 0.0024 0.0007 0.0002 0.0009 0.0002 0.001 0.0041 0.0032 0.0001 0 0.0001 0.0019 0.0005 0.0028 0.0001 0.0001 0.0001
Cell Periphery 0.0002 0.0002 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0.0003 0.0001 0.0002 0 0 0
Cytoplasm 0.7334 0.8837 0.7922 0.8912 0.7537 0.8987 0.825 0.9687 0.9445 0.9713 0.9808 0.9476 0.8777 0.959 0.8757 0.8868 0.9222 0.9393
Cytoplasmic Foci 0.0278 0.0088 0.0117 0.0058 0.0253 0.0121 0.0205 0.0042 0.0062 0.0073 0.0043 0.0144 0.0132 0.0022 0.0126 0.0031 0.0023 0.004
Eisosomes 0.0002 0.0001 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0 0 0.0002 0 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.007 0.0038 0.0052 0.0026 0.0045 0.0045 0.0064 0.0016 0.0018 0.0021 0.0015 0.0018 0.0042 0.001 0.0018 0.0015 0.0033 0.0014
Endosome 0.03 0.0061 0.0116 0.007 0.0238 0.011 0.0261 0.0013 0.0031 0.0048 0.0023 0.0053 0.0151 0.0006 0.0052 0.0018 0.0037 0.0021
Golgi 0.0044 0.0011 0.0031 0.0009 0.0069 0.0006 0.0044 0.0001 0.0015 0.0002 0.0001 0.0008 0.0026 0.0001 0.0031 0.0001 0.0033 0.0012
Lipid Particles 0.008 0.0041 0.0031 0.0001 0.022 0.0002 0.0107 0.0001 0.0036 0 0 0.0027 0.0023 0 0.0032 0 0.001 0.0011
Mitochondria 0.0034 0.0004 0.0019 0.0003 0.0038 0.0003 0.0035 0.0001 0.0011 0.0001 0.0001 0.0002 0.0036 0.0001 0.0045 0.0001 0.0003 0.0003
None 0.1149 0.0684 0.1117 0.0252 0.0448 0.0434 0.0259 0.0152 0.0107 0.0067 0.0059 0.0091 0.0283 0.0324 0.0633 0.1002 0.0265 0.0361
Nuclear Periphery 0.0093 0.0033 0.0103 0.0008 0.0193 0.0025 0.0147 0.0005 0.001 0.001 0.0007 0.0008 0.0052 0.0005 0.0009 0.0006 0.0031 0.0013
Nucleolus 0.0005 0.0005 0.0002 0 0.001 0.0001 0.0005 0 0.0001 0 0 0 0.0004 0 0.0001 0 0 0.0001
Nucleus 0.0143 0.0046 0.0055 0.0025 0.0224 0.0049 0.0047 0.0023 0.0028 0.0035 0.0027 0.0051 0.006 0.0021 0.0028 0.0038 0.0038 0.0026
Peroxisomes 0.0058 0.0024 0.0038 0.0004 0.0416 0.0008 0.0061 0 0.0061 0.0001 0 0.0073 0.0013 0 0.0132 0 0.0001 0.0004
Punctate Nuclear 0.0159 0.0033 0.0026 0.0004 0.0184 0.0062 0.0101 0.0003 0.0015 0.0004 0.0004 0.0016 0.0044 0.0002 0.0013 0.0003 0.001 0.001
Vacuole 0.0033 0.0015 0.0012 0.0007 0.0013 0.0013 0.0019 0.0006 0.0004 0.0014 0.0006 0.0005 0.0018 0.0004 0.0008 0.0006 0.0003 0.0003
Vacuole Periphery 0.0011 0.0003 0.0008 0.0002 0.0009 0.0003 0.0018 0.0001 0.0001 0.0002 0.0001 0.0001 0.0005 0 0.0002 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.8765 1.6775 6.2239 19.6408 1.2165 13.1285 12.2969 18.3164 23.6037 12.2712
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1721 499 192 2138 1282 2281 736 1347 3003 2780 928 3485

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 711.43 737.44 1201.92 892.45 719.49 816.64 801.61 940.24 714.87 802.42 884.43 910.92
Standard Deviation 110.40 133.76 161.00 125.18 92.81 106.04 131.59 152.39 103.34 115.59 213.05 138.32
Intensity Change Log 2 — 0.051804 0.756547 0.327050 — 0.182726 0.155926 0.386054 — 0.119118 0.485601 0.357020

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000122 0.000426 0.000885 0.001880 0.000272 0.000240 0.000271 0.001332 0.000186 0.000273 0.000398 0.001668
Bud Neck 0.012406 0.009856 0.003270 0.005484 0.007069 0.012383 0.000588 0.005986 0.010128 0.011929 0.001142 0.005678
Bud Site 0.004830 0.013271 0.007643 0.026864 0.003582 0.013434 0.004175 0.034795 0.004297 0.013405 0.004892 0.029929
Cell Periphery 0.000145 0.000150 0.000320 0.000101 0.000111 0.000066 0.000123 0.000149 0.000131 0.000081 0.000164 0.000119
Cytoplasm 0.536233* 0.574093* 0.476354* 0.598536* 0.619340* 0.667450* 0.672891* 0.637607* 0.571712* 0.650693* 0.632228* 0.613638*
Cytoplasmic Foci 0.203802* 0.180435* 0.020595 0.014860 0.166040 0.094505 0.013428 0.022561 0.187681* 0.109929 0.014911 0.017837
Eisosomes 0.000138 0.000085 0.000064 0.000025 0.000082 0.000041 0.000030 0.000025 0.000114 0.000049 0.000037 0.000025
Endoplasmic Reticulum 0.000892 0.001171 0.006550 0.000998 0.000752 0.000411 0.001885 0.001425 0.000832 0.000548 0.002850 0.001163
Endosome 0.010102 0.019207 0.008165 0.005749 0.006682 0.005387 0.002199 0.012298 0.008642 0.007867 0.003434 0.008280
Golgi 0.002431 0.002593 0.000330 0.004056 0.001166 0.001252 0.000106 0.005368 0.001891 0.001493 0.000152 0.004563
Lipid Particles 0.006881 0.003670 0.002956 0.000588 0.003838 0.001229 0.000940 0.002188 0.005582 0.001667 0.001357 0.001207
Mitochondria 0.004515 0.002134 0.000354 0.006882 0.003471 0.002676 0.000155 0.001624 0.004069 0.002578 0.000197 0.004849
Mitotic Spindle 0.001321 0.001303 0.005195 0.085882 0.002962 0.008500 0.002714 0.047547 0.002022 0.007208 0.003227 0.071065
None 0.008032 0.010608 0.001926 0.007124 0.003703 0.003445 0.007789 0.004970 0.006184 0.004731 0.006576 0.006292
Nuclear Periphery 0.000434 0.000304 0.001844 0.000874 0.000285 0.000207 0.000404 0.000881 0.000370 0.000225 0.000702 0.000877
Nuclear Periphery Foci 0.000636 0.001472 0.010543 0.000775 0.000532 0.000256 0.004243 0.002356 0.000592 0.000474 0.005546 0.001386
Nucleolus 0.001440 0.002307 0.000286 0.000267 0.000549 0.000666 0.000350 0.000243 0.001060 0.000960 0.000337 0.000258
Nucleus 0.146411* 0.101354 0.250143* 0.138868* 0.135826* 0.130305* 0.217457* 0.116698 0.141892* 0.125108 0.224220* 0.130299*
Peroxisomes 0.002928 0.005016 0.000334 0.000649 0.002323 0.002710 0.000193 0.002895 0.002670 0.003124 0.000222 0.001517
Vacuole 0.055434 0.068640 0.201887* 0.096688 0.040749 0.053957 0.069928 0.095394 0.049165 0.056593 0.097230 0.096188
Vacuole Periphery 0.000867 0.001905 0.000354 0.002851 0.000668 0.000879 0.000133 0.003659 0.000782 0.001063 0.000178 0.003163

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.72 -9.72 -1.65 -0.62 0.92 0.10 -0.94 -1.81 -1.95 -1.54 -1.71 -6.11 -2.20 -1.70 -0.84
Bud Neck 1.45 7.25 7.67 3.57 0.20 -4.20 7.23 3.30 8.32 -8.13 -1.64 12.01 8.23 9.27 -10.58
Bud Site -4.91 -3.84 -5.24 0.53 -2.20 -7.56 -0.84 -6.98 -1.97 -6.07 -8.16 -1.24 -8.65 -0.71 -6.72
Cell Periphery -0.32 -6.12 8.31 5.78 9.54 8.69 -1.89 0.24 -4.42 1.63 7.87 -3.44 6.96 -0.40 8.59
Cytoplasm -2.35 8.36 2.56 3.91 -7.21 -5.29 1.46 7.84 13.49 5.08 -11.15 1.53 7.24 18.10 3.19
Cytoplasmic Foci 2.40 37.82 40.81 19.60 3.94 12.78 31.14 30.47 25.97 -2.63 17.46 49.67 50.76 33.16 1.12
Eisosomes 3.97 8.36 15.50 5.03 6.26 7.99 8.23 10.03 3.72 1.67 12.48 13.94 18.31 7.13 5.25
Endoplasmic Reticulum -1.70 -8.87 -1.44 0.86 8.67 2.95 -8.65 -5.19 -9.48 5.12 3.25 -11.96 -4.44 -8.81 10.36
Endosome -3.58 3.11 10.60 6.93 6.20 1.96 7.77 1.89 0.54 -3.13 1.12 10.54 9.34 6.80 -0.05
Golgi -0.12 5.08 2.18 1.80 -2.27 -0.24 6.30 -1.20 -1.02 -3.34 1.27 6.97 1.17 0.09 -4.15
Lipid Particles 4.81 6.22 11.25 6.82 9.31 6.54 8.00 5.29 -0.50 -1.03 9.92 11.41 12.53 2.77 3.22
Mitochondria 2.09 4.13 1.81 -0.43 -3.42 0.61 2.72 2.15 3.55 -4.50 1.73 5.01 2.65 1.33 -4.36
Mitotic Spindle 0.07 -1.96 -10.98 -11.00 -5.07 -3.03 0.29 -7.02 -4.82 -7.07 -3.91 -1.38 -12.94 -9.51 -10.71
None -0.79 4.33 0.42 1.18 -9.99 0.44 -3.97 -2.88 -3.65 1.79 1.58 0.78 -0.49 -3.14 -2.27
Nuclear Periphery 2.11 -12.84 -15.02 -18.77 4.85 1.96 -7.91 -14.05 -16.51 -8.85 3.94 -11.41 -20.13 -26.19 -7.44
Nuclear Periphery Foci -4.43 -9.29 -1.51 3.79 9.16 3.10 -10.66 -7.86 -8.72 5.66 1.05 -13.66 -7.41 -7.56 11.54
Nucleolus -1.27 6.82 6.61 3.50 -1.35 -1.44 4.38 6.06 4.71 2.15 0.75 7.42 7.65 5.41 0.54
Nucleus 5.86 -7.39 -10.52 -13.11 4.08 0.93 -11.99 -4.51 -5.64 8.66 4.01 -13.85 -11.35 -14.57 7.70
Peroxisomes -2.37 10.37 10.55 5.45 1.13 -0.81 6.09 2.82 3.89 -2.82 -1.28 11.80 8.90 8.00 -2.76
Vacuole -3.30 -12.99 -25.45 -17.90 4.09 -5.28 -11.31 -24.34 -22.36 -10.08 -4.14 -15.06 -35.25 -32.31 -7.13
Vacuole Periphery -3.06 5.51 0.03 2.09 -1.52 -0.92 3.52 -1.51 -1.02 -3.07 -1.61 7.10 -0.85 0.07 -3.21
Standard name
Human Ortholog
Description Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Igd1

Igd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Igd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available