Standard name
Human Ortholog
Description Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.73 0.92 0.79 0.63 0.71 0.76 0.68 0.73 0.58 0.7 0.52 0.6 0.93 0.98 1.0 0.46 0.44 0.45 0.47 0.6 0.56 0.7 0.78 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.09 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0.09 0.18 0.06 0.2 0.38 0.29 0.49 0.36 0.65 0.47 0 0 0 0 0.05 0 0.1 0.13 0.12 0 0.07 0.07
Nucleus 0.11 0 0.12 0.19 0.12 0 0 0.05 0.08 0 0 0.05 0 0 0 0.26 0.23 0.25 0.14 0.07 0.09 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0.05 0 0 0.05
Vac/Vac Membrane 0.09 0.1 0.07 0.05 0.14 0.07 0.05 0 0 0 0 0 0.05 0 0 0.25 0.29 0.25 0.12 0.1 0.1 0.09 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 2 2 0 4 4 8
Bud 0 0 0 1 3 0 6 6 8 13 11 0 0 0 0 0 0 1 4 1 0 4 5 9
Bud Neck 0 0 1 2 9 2 3 12 9 10 1 3 2 0 2 2 1 0 0 0 0 1 2 3
Bud Site 0 0 0 0 0 0 1 1 7 1 3 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 3 1 0 1 0 0 0 0 1 2 0 0 0 1 0 1 2 3
Cytoplasm 138 107 59 92 166 169 308 303 212 215 179 198 286 447 462 131 68 82 90 78 58 159 283 320
Endoplasmic Reticulum 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 17 5 4 3 0 0 2 0 1
Endosome 0 0 1 2 1 0 1 1 1 2 0 0 2 1 0 18 12 16 4 1 1 1 2 5
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 1 0 2
Mitochondria 43 5 7 27 14 45 174 119 177 111 222 156 1 2 0 3 7 6 18 16 12 10 23 30
Nucleus 20 3 9 28 29 8 19 20 28 13 14 17 8 1 2 73 35 46 27 9 9 6 1 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1 6 7 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1
SpindlePole 1 0 0 3 2 0 8 5 4 9 2 3 0 0 0 8 11 11 8 1 5 7 13 19
Vac/Vac Membrane 17 12 5 8 32 16 23 11 11 4 15 11 15 8 1 70 45 45 23 13 10 20 13 5
Unique Cell Count 190 116 75 146 233 221 452 414 363 309 344 329 306 455 463 285 154 183 192 131 104 229 363 426
Labelled Cell Count 221 127 83 164 257 241 546 480 458 380 448 390 314 459 469 328 190 219 192 131 104 229 363 426


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.2 4.3 4.8 4.7 3.9 3.5 4.0 3.4 3.4 3.1 3.4 4.9 4.9 4.8 5.8 6.4 5.7 4.6 5.1 4.8
Std Deviation (1e-4) 2.0 0.9 0.9 1.9 1.3 1.5 1.1 1.4 1.4 1.1 1.2 1.0 1.1 1.1 1.2 1.3 2.0 1.1 1.3 2.7 1.2
Intensity Change (Log2) 0.16 0.11 -0.15 -0.32 -0.14 -0.36 -0.33 -0.47 -0.34 0.18 0.18 0.14 0.41 0.55 0.39 0.07 0.24 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.4 3.9 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3735 -0.8138 -0.9815 -1.1103 -1.3181 -0.7195 -0.0485 -0.2069 -0.6184 -1.2005 -0.7211 -0.3184 -0.6653 -1.1625 -1.4128 -1.0406 -1.8446 -1.0747
Actin 0.0163 0.0003 0.005 0.0007 0.0163 0.0032 0.0114 0.0017 0.0052 0.0006 0.0022 0.0042 0.0244 0.0004 0.0076 0.0057 0.0019 0.0051
Bud 0.0005 0.0014 0.0025 0.0002 0.0008 0.0001 0.0003 0.0012 0.0008 0.0006 0.0002 0.0004 0.0024 0.0007 0.0009 0.0204 0.0004 0.0028
Bud Neck 0.0037 0.0008 0.0017 0.0001 0.0016 0.0059 0.0017 0.0006 0.0009 0.0205 0.0009 0.0062 0.0166 0.0008 0.001 0.0009 0.0009 0.008
Bud Periphery 0.0011 0.0015 0.0034 0.0004 0.0028 0.0002 0.0004 0.0018 0.0007 0.001 0.0001 0.0012 0.0014 0.0005 0.0013 0.0022 0.0005 0.0013
Bud Site 0.0084 0.0061 0.0077 0.0001 0.0016 0.0002 0.0011 0.0018 0.0016 0.0017 0.0053 0.0003 0.0203 0.0154 0.0101 0.002 0.0003 0.0021
Cell Periphery 0.0004 0.0002 0.0002 0 0.0002 0 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0004 0.0001 0.0003 0.0001 0 0.0002
Cytoplasm 0.071 0.0552 0.0442 0.0602 0.0404 0.0764 0.0834 0.153 0.0919 0.095 0.1152 0.1103 0.1407 0.1187 0.1293 0.1122 0.1472 0.1498
Cytoplasmic Foci 0.0255 0.007 0.0048 0.0087 0.0023 0.0096 0.0201 0.0044 0.009 0.0186 0.0211 0.0066 0.02 0.0029 0.0147 0.0115 0.0096 0.01
Eisosomes 0.0005 0.0001 0.0002 0.0002 0.0004 0.0001 0.0002 0.0008 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0021 0.0011 0.0011 0.0006 0.0012 0.0013 0.0027 0.002 0.003 0.002 0.0014 0.0013 0.0039 0.0019 0.0033 0.001 0.0009 0.0016
Endosome 0.0147 0.0016 0.0026 0.0009 0.0014 0.004 0.0145 0.002 0.0043 0.0062 0.0082 0.0052 0.0211 0.0015 0.0066 0.0037 0.0041 0.006
Golgi 0.0047 0.0002 0.0007 0.0001 0.0005 0.0007 0.0042 0.0003 0.0011 0.0014 0.0014 0.001 0.0079 0.0001 0.0026 0.0059 0.0024 0.0008
Lipid Particles 0.0172 0.0021 0.0008 0.0006 0.0007 0.005 0.0285 0.001 0.0025 0.0024 0.0025 0.0014 0.0111 0.0002 0.0028 0.015 0.0029 0.0029
Mitochondria 0.0092 0.0025 0.0015 0.0004 0.0013 0.0008 0.0058 0.0013 0.0013 0.0013 0.0004 0.0023 0.0057 0.0006 0.0054 0.007 0.0274 0.0024
None 0.4883 0.6979 0.8381 0.9037 0.9073 0.5265 0.3703 0.5409 0.7216 0.7677 0.7565 0.3979 0.3267 0.4812 0.5943 0.6603 0.6786 0.3436
Nuclear Periphery 0.0089 0.0051 0.0066 0.003 0.0046 0.0072 0.0116 0.0071 0.0092 0.0053 0.0018 0.0062 0.0102 0.0109 0.009 0.0023 0.002 0.0068
Nucleolus 0.0039 0.004 0.0023 0.0005 0.0009 0.0045 0.0033 0.0033 0.0022 0.0043 0.0005 0.0039 0.0041 0.0028 0.0069 0.0018 0.0012 0.0042
Nucleus 0.2642 0.1929 0.0571 0.0074 0.009 0.2849 0.3886 0.2479 0.1184 0.0395 0.0644 0.4221 0.3617 0.3373 0.1779 0.0958 0.0768 0.4201
Peroxisomes 0.0266 0.0018 0.0022 0.0008 0.0005 0.0026 0.0195 0.0012 0.0076 0.0184 0.0024 0.0022 0.0075 0.0002 0.0064 0.0288 0.0015 0.0028
Punctate Nuclear 0.0295 0.0163 0.0156 0.011 0.0052 0.0661 0.0303 0.0247 0.0157 0.0081 0.0147 0.0255 0.0079 0.021 0.0157 0.0226 0.0401 0.0278
Vacuole 0.0027 0.0015 0.0012 0.0003 0.0005 0.0005 0.0012 0.0024 0.0021 0.0044 0.0006 0.0012 0.0049 0.0025 0.0032 0.0006 0.0007 0.0013
Vacuole Periphery 0.0006 0.0002 0.0004 0.0001 0.0003 0.0003 0.0007 0.0004 0.0005 0.0008 0.0001 0.0004 0.0009 0.0004 0.0007 0.0002 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.3148 46.22 25.7229 36.2636 30.471 38.7563 45.4053 37.9933 42.6579 36.5469
Translational Efficiency 1.4648 1.3142 2.2614 1.7069 1.983 1.7487 1.2198 1.8165 1.3258 1.2433

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
470 888 1271 502 1932 1495 163 818 2402 2383 1434 1320

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 662.49 678.16 771.72 789.71 651.65 688.10 952.80 832.27 653.77 684.40 792.30 816.08
Standard Deviation 68.94 106.39 94.78 101.01 77.93 83.79 99.64 109.44 76.38 92.98 111.33 108.30
Intensity Change Log 2 0.033727 0.220179 0.253424 0.078521 0.548076 0.352954 0.056113 0.392076 0.303637

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000110 0.000526 0.000124 0.000305 0.000058 0.000234 0.003402 0.000551 0.000068 0.000343 0.000497 0.000457
Bud Neck 0.013549 0.033669 0.005753 0.003791 0.010467 0.025376 0.001765 0.005845 0.011070 0.028466 0.005300 0.005064
Bud Site 0.002033 0.018729 0.004358 0.006211 0.003867 0.007798 0.003193 0.026004 0.003508 0.011872 0.004225 0.018477
Cell Periphery 0.000220 0.000199 0.000460 0.000223 0.000231 0.000202 0.000261 0.000169 0.000229 0.000201 0.000438 0.000189
Cytoplasm 0.337596* 0.329600* 0.297072* 0.285625* 0.419133* 0.375512* 0.124079* 0.361546* 0.403179* 0.358403* 0.277408* 0.332673*
Cytoplasmic Foci 0.244375* 0.218014* 0.014316 0.022303 0.124340 0.199983* 0.021129 0.024181 0.147827 0.206702* 0.015090 0.023467
Eisosomes 0.000229 0.000166 0.000097 0.000063 0.000135 0.000152 0.000141 0.000050 0.000153 0.000157 0.000102 0.000055
Endoplasmic Reticulum 0.000728 0.001214 0.001689 0.007169 0.001300 0.000632 0.007271 0.003065 0.001188 0.000849 0.002323 0.004626
Endosome 0.008862 0.022203 0.001374 0.004635 0.004325 0.012967 0.005684 0.005870 0.005213 0.016409 0.001864 0.005400
Golgi 0.001485 0.004295 0.000066 0.001685 0.001139 0.004494 0.001257 0.003680 0.001207 0.004419 0.000201 0.002922
Lipid Particles 0.012947 0.008761 0.001080 0.003229 0.007739 0.007793 0.005000 0.001757 0.008758 0.008154 0.001526 0.002317
Mitochondria 0.004337 0.017385 0.000828 0.001191 0.005800 0.013101 0.000690 0.003920 0.005513 0.014697 0.000812 0.002882
Mitotic Spindle 0.000421 0.004655 0.003567 0.014334 0.001518 0.005508 0.006212 0.055664 0.001303 0.005190 0.003868 0.039946
None 0.004102 0.007500 0.010216 0.004428 0.004183 0.005951 0.002280 0.008032 0.004167 0.006528 0.009314 0.006662
Nuclear Periphery 0.000915 0.000901 0.001147 0.002333 0.000768 0.000509 0.002076 0.001858 0.000797 0.000655 0.001252 0.002039
Nuclear Periphery Foci 0.000336 0.000798 0.000314 0.002810 0.000305 0.000341 0.002102 0.001223 0.000311 0.000511 0.000517 0.001827
Nucleolus 0.001593 0.004570 0.001109 0.000926 0.001860 0.003008 0.001061 0.001394 0.001807 0.003590 0.001103 0.001216
Nucleus 0.324855* 0.249822* 0.635471* 0.552858* 0.384868* 0.282812* 0.693586* 0.414314* 0.373125* 0.270518* 0.642076* 0.467002*
Peroxisomes 0.011243 0.022707 0.000304 0.000487 0.002488 0.016168 0.001693 0.001219 0.004201 0.018605 0.000462 0.000940
Vacuole 0.029481 0.051802 0.020483 0.085005 0.024878 0.036175 0.114651 0.074135 0.025778 0.041998 0.031187 0.078269
Vacuole Periphery 0.000585 0.002484 0.000174 0.000388 0.000599 0.001286 0.002465 0.005524 0.000596 0.001732 0.000435 0.003571

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.11 -2.31 -8.01 2.15 -5.19 -7.35 -1.30 -7.69 -5.41 0.91 -8.10 -1.77 -9.37 -3.57 -0.85
Bud Neck -6.17 3.04 3.69 10.36 0.95 -7.84 10.99 0.75 8.08 -8.69 -10.66 4.45 3.13 12.80 -1.92
Bud Site -7.81 -2.72 -3.18 5.26 -0.84 -3.93 0.43 -5.05 -3.63 -4.99 -7.88 -1.09 -5.42 -0.85 -4.58
Cell Periphery 1.26 -5.71 2.18 1.28 6.83 1.33 -0.89 2.95 2.65 4.72 1.57 -4.63 3.20 2.87 8.21
Cytoplasm 0.62 4.03 6.21 6.35 3.26 4.80 21.80 15.07 10.23 -8.92 5.95 16.07 17.52 12.02 1.43
Cytoplasmic Foci 2.20 22.08 21.26 26.36 -2.49 -11.13 22.70 25.65 30.80 0.60 -9.98 34.31 32.41 40.44 -2.33
Eisosomes 2.71 6.50 7.50 6.57 1.84 -1.27 -2.24 3.18 5.19 5.34 -0.28 4.73 6.49 8.17 1.41
Endoplasmic Reticulum -3.28 -8.54 -10.22 -9.42 -7.74 1.66 -8.61 -9.26 -14.74 3.66 1.00 -5.59 -12.14 -16.83 -9.10
Endosome -5.96 6.54 3.76 9.22 -5.09 -7.45 -2.33 -2.36 5.41 0.24 -10.62 8.36 -0.19 10.17 -7.03
Golgi -4.96 4.52 0.87 4.06 -1.08 -4.72 0.77 -0.19 3.54 -0.67 -6.52 6.79 0.24 5.05 -2.08
Lipid Particles 2.36 7.53 6.39 6.10 -4.32 -0.08 2.72 8.39 6.10 5.10 0.75 12.73 10.49 8.47 -4.18
Mitochondria -5.16 3.80 3.71 7.03 -0.19 -3.40 4.45 3.20 6.16 -2.48 -5.30 4.86 4.06 9.14 -1.93
Mitotic Spindle -4.09 -2.81 -4.31 -3.49 -3.57 -2.96 -0.81 -7.92 -7.06 -6.99 -4.02 -2.17 -8.92 -7.82 -8.00
None -2.35 -3.27 0.23 3.02 3.94 -2.02 4.06 -3.23 -1.10 -5.69 -3.19 -3.56 -2.68 0.63 1.90
Nuclear Periphery 0.03 -1.92 -6.13 -6.81 -5.98 3.23 -8.27 -13.76 -15.09 -3.92 1.37 -7.96 -14.42 -15.07 -10.82
Nuclear Periphery Foci -2.33 -1.07 -5.19 -4.34 -5.09 -0.49 -6.72 -5.97 -5.93 -2.90 -2.30 -5.05 -7.63 -7.25 -7.01
Nucleolus -5.40 1.81 -0.23 4.72 -1.54 -2.09 3.08 -3.65 -2.37 -4.50 -4.10 2.49 -3.52 -0.98 -4.50
Nucleus 4.48 -20.34 -9.92 -15.53 7.58 10.32 -10.36 -2.68 -10.18 8.44 12.28 -27.63 -7.91 -17.44 15.75
Peroxisomes -4.39 6.41 6.29 11.11 -1.84 -9.91 2.13 2.24 10.33 -0.30 -12.03 9.77 6.79 15.04 -2.63
Vacuole -5.90 0.31 -12.79 -10.18 -13.01 -5.24 -8.84 -19.26 -17.65 -0.61 -8.57 -6.19 -23.28 -20.26 -18.56
Vacuole Periphery -5.49 5.15 3.57 6.56 -1.64 -4.21 -0.72 -1.29 -0.51 -0.15 -6.78 1.72 -1.05 0.94 -1.51
Standard name
Human Ortholog
Description Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability
Localization
Cell Percentages cytoplasm (20%), nucleus (13%), mixed (43%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00066)
Subcompartmental Group N/A

Pho4

Pho4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available