Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 |
Bud | 2 | 0 | 9 | 10 | 2 | 6 | – | 31 | 11 | 18 | 19 | 11 | 0 | 0 | 0 | 1 | 0 | 0 | 14 | 5 | 11 | 13 | 13 | 13 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 3 | – | 0 | 3 | 3 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 2 | 2 | 5 | 3 |
Bud Site | 0 | 1 | 3 | 15 | 9 | 29 | – | 28 | 14 | 32 | 21 | 27 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 58 | 21 | 61 | 37 | 28 | 36 | – | 63 | 62 | 79 | 80 | 107 | 56 | 80 | 124 | 26 | 30 | 25 | 24 | 15 | 21 | 36 | 30 | 31 |
Endoplasmic Reticulum | 3 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 8 | 5 | 4 | 1 | 0 | 3 | 1 | 1 | 1 |
Endosome | 8 | 7 | 3 | 12 | 41 | 12 | – | 13 | 1 | 9 | 2 | 4 | 10 | 20 | 24 | 20 | 9 | 13 | 21 | 6 | 21 | 8 | 13 | 12 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 4 | 1 | 2 | 2 | 0 | 2 |
Mitochondria | 152 | 24 | 293 | 205 | 84 | 260 | – | 496 | 402 | 416 | 337 | 404 | 11 | 8 | 6 | 17 | 17 | 36 | 15 | 2 | 21 | 9 | 17 | 19 |
Nucleus | 439 | 117 | 283 | 164 | 127 | 213 | – | 319 | 219 | 205 | 109 | 123 | 175 | 175 | 179 | 144 | 141 | 149 | 416 | 124 | 270 | 120 | 123 | 102 |
Nuclear Periphery | 4 | 1 | 2 | 7 | 6 | 10 | – | 10 | 8 | 4 | 2 | 2 | 2 | 8 | 9 | 5 | 3 | 4 | 6 | 1 | 11 | 1 | 2 | 2 |
Nucleolus | 18 | 14 | 42 | 81 | 76 | 130 | – | 158 | 66 | 78 | 43 | 37 | 36 | 41 | 37 | 42 | 24 | 41 | 46 | 16 | 49 | 12 | 10 | 20 |
Peroxisomes | 0 | 0 | 0 | 0 | 5 | 3 | – | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 10 | 3 | 23 | 33 | 58 | 69 | – | 54 | 24 | 44 | 23 | 19 | 6 | 14 | 6 | 23 | 13 | 21 | 61 | 20 | 85 | 25 | 29 | 33 |
Vac/Vac Membrane | 43 | 31 | 19 | 9 | 24 | 26 | – | 46 | 41 | 34 | 23 | 21 | 19 | 24 | 31 | 14 | 22 | 11 | 91 | 25 | 95 | 42 | 59 | 60 |
Unique Cell Count | 631 | 196 | 523 | 383 | 350 | 556 | 894 | 610 | 631 | 475 | 547 | 290 | 334 | 380 | 234 | 219 | 239 | 728 | 226 | 616 | 286 | 313 | 320 | |
Labelled Cell Count | 738 | 219 | 739 | 575 | 461 | 797 | 1219 | 851 | 924 | 660 | 761 | 316 | 373 | 423 | 301 | 265 | 306 | 728 | 226 | 616 | 286 | 313 | 320 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.2 | 3.8 | 4.0 | 4.9 | 4.0 | – | 3.8 | 3.4 | 3.6 | 3.5 | 3.1 | 5.3 | 5.7 | 5.5 | 7.4 | 7.1 | 7.0 | 5.3 | 5.3 | 5.1 |
Std Deviation (1e-4) | 0.8 | 1.1 | 0.9 | 1.6 | 1.5 | 1.1 | – | 1.2 | 1.1 | 1.2 | 1.2 | 0.7 | 1.2 | 1.4 | 1.5 | 1.9 | 1.9 | 1.9 | 1.4 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.37 | 0.07 | – | 0.01 | -0.15 | -0.1 | -0.14 | -0.28 | 0.49 | 0.58 | 0.53 | 0.96 | 0.91 | 0.87 | 0.49 | 0.48 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -1.8 | -3.0 | – | -3.0 | -0.8 | 0.4 | 2.3 | 3.5 | 3.0 | 4.7 | 7.7 | -0.2 | 0.8 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 7.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 5.0 | 5.9 | 0 | 4.3 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -9.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | -14.7 | -16.0 | -17.2 | -12.6 | -12.2 | -10.6 |
Nucleus | -3.4 | -5.2 | -5.2 | – | -6.8 | -6.2 | -7.4 | -10.1 | -10.7 | 1.7 | -0.5 | -2.1 | 1.9 | 2.6 | 2.1 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 5.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.0 | 2.0 | 0.9 | 4.0 | 1.3 | 3.8 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 6.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | -1.7 | -0.1 | -2.4 | 2.9 | 0.9 | 2.4 |
Vacuole | -1.1 | 2.2 | 0.9 | – | 1.3 | 2.3 | 1.4 | 1.0 | 0.2 | 1.9 | 2.3 | 2.9 | 1.5 | 3.5 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0977 | 1.7018 | 1.7434 | 1.0248 | 1.6973 | 1.5008 | 0.7216 | 0.6208 | 0.7591 | 0.5187 | 1.5625 | 0.767 | 1.3553 | 2.3267 | 1.8923 | 2.074 | 1.6132 | 1.9018 |
Actin | 0.0261 | 0.0002 | 0.0187 | 0.0002 | 0.0324 | 0.0065 | 0.0081 | 0.0001 | 0.0159 | 0.001 | 0.0076 | 0.0023 | 0.0021 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 |
Bud | 0.0008 | 0.0002 | 0.0004 | 0.0001 | 0.0012 | 0.0002 | 0.0002 | 0.0001 | 0.0023 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0001 |
Bud Neck | 0.0045 | 0.0007 | 0.0062 | 0.0013 | 0.0015 | 0.0043 | 0.0014 | 0.0021 | 0.0013 | 0.0006 | 0.0022 | 0.0025 | 0.0008 | 0.0006 | 0.001 | 0.0002 | 0.0005 | 0.0031 |
Bud Periphery | 0.0014 | 0.0001 | 0.0007 | 0.0001 | 0.0031 | 0.0003 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.001 | 0.0001 |
Bud Site | 0.005 | 0.0008 | 0.0051 | 0.0002 | 0.0059 | 0.0002 | 0.0007 | 0.0005 | 0.0033 | 0.0009 | 0.0009 | 0.0002 | 0.0003 | 0.0007 | 0.0004 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.0003 | 0 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0234 | 0.0314 | 0.0107 | 0.0205 | 0.0009 | 0.0298 | 0.0218 | 0.0079 | 0.0051 | 0.0127 | 0.0046 | 0.0187 | 0.0153 | 0.0295 | 0.0127 | 0.0027 | 0.0079 | 0.0201 |
Cytoplasmic Foci | 0.0259 | 0.0015 | 0.0057 | 0.0009 | 0.0029 | 0.0013 | 0.0114 | 0.0012 | 0.005 | 0.0065 | 0.0038 | 0.003 | 0.0012 | 0.0018 | 0.0022 | 0 | 0.001 | 0.0012 |
Eisosomes | 0.0002 | 0 | 0.0006 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0005 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0046 | 0.0021 | 0.002 | 0.0017 | 0.0014 | 0.0015 | 0.0056 | 0.0006 | 0.0014 | 0.0087 | 0.0039 | 0.0007 | 0.0012 | 0.0017 | 0.0006 | 0.0001 | 0.0004 | 0.0005 |
Endosome | 0.0314 | 0.0024 | 0.007 | 0.0023 | 0.0077 | 0.0063 | 0.0164 | 0.001 | 0.0043 | 0.0219 | 0.0228 | 0.0049 | 0.0015 | 0.0009 | 0.0013 | 0 | 0.0004 | 0.0012 |
Golgi | 0.0095 | 0.0001 | 0.003 | 0.0001 | 0.0041 | 0.0017 | 0.0033 | 0.0001 | 0.0021 | 0.007 | 0.0049 | 0.0026 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Lipid Particles | 0.0182 | 0.0009 | 0.0071 | 0.0004 | 0.0051 | 0.0012 | 0.0174 | 0.0004 | 0.0048 | 0.0011 | 0.0221 | 0.0054 | 0.0005 | 0.0004 | 0.0004 | 0 | 0.0001 | 0.0001 |
Mitochondria | 0.0084 | 0.0005 | 0.0021 | 0.0004 | 0.0095 | 0.0008 | 0.0074 | 0.0002 | 0.003 | 0.0081 | 0.0065 | 0.0031 | 0.0012 | 0.0003 | 0.0002 | 0.0002 | 0.0008 | 0.0002 |
None | 0.0216 | 0.019 | 0.027 | 0.0042 | 0.0009 | 0.0137 | 0.0321 | 0.0106 | 0.0233 | 0.0073 | 0.0748 | 0.0234 | 0.0423 | 0.0812 | 0.0142 | 0.0021 | 0.0815 | 0.01 |
Nuclear Periphery | 0.0404 | 0.0178 | 0.0439 | 0.0273 | 0.0345 | 0.0123 | 0.0554 | 0.0201 | 0.0445 | 0.0178 | 0.0724 | 0.0092 | 0.0193 | 0.0097 | 0.0276 | 0.0031 | 0.0031 | 0.0083 |
Nucleolus | 0.0279 | 0.0354 | 0.047 | 0.0194 | 0.0727 | 0.053 | 0.0518 | 0.0695 | 0.0735 | 0.0168 | 0.0798 | 0.0712 | 0.0387 | 0.0448 | 0.0424 | 0.0085 | 0.0286 | 0.046 |
Nucleus | 0.5887 | 0.7435 | 0.6299 | 0.8378 | 0.7383 | 0.7913 | 0.5627 | 0.7204 | 0.6309 | 0.7461 | 0.6144 | 0.7544 | 0.6697 | 0.6732 | 0.6202 | 0.9734 | 0.6723 | 0.7981 |
Peroxisomes | 0.0167 | 0.0001 | 0.0047 | 0.0001 | 0.0029 | 0.0003 | 0.0052 | 0.0001 | 0.0048 | 0.0018 | 0.0125 | 0.0031 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.1375 | 0.1404 | 0.1752 | 0.0808 | 0.0673 | 0.0726 | 0.1923 | 0.164 | 0.1711 | 0.1395 | 0.059 | 0.0939 | 0.203 | 0.1527 | 0.2748 | 0.0093 | 0.2004 | 0.1097 |
Vacuole | 0.0049 | 0.0025 | 0.0014 | 0.0016 | 0.0031 | 0.0022 | 0.0039 | 0.0008 | 0.0012 | 0.0012 | 0.0031 | 0.0006 | 0.0015 | 0.0016 | 0.0009 | 0.0001 | 0.0007 | 0.0006 |
Vacuole Periphery | 0.0026 | 0.0004 | 0.0009 | 0.0005 | 0.0042 | 0.0004 | 0.0021 | 0.0002 | 0.0007 | 0.0007 | 0.0033 | 0.0003 | 0.0006 | 0.0003 | 0.0002 | 0 | 0.0003 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.2409 | 13.1517 | 1.9518 | 8.327 | 13.3524 | 17.6447 | 6.6488 | 8.975 | 7.0273 | 10.3246 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1845 | 145 | 1373 | 1145 | 1743 | 396 | 2855 | 1094 | 3588 | 541 | 4228 | 2239 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 686.10 | 759.11 | 902.16 | 876.49 | 671.19 | 733.02 | 892.74 | 918.62 | 678.86 | 740.01 | 895.80 | 897.08 |
Standard Deviation | 85.89 | 105.02 | 118.97 | 159.16 | 83.47 | 106.12 | 132.21 | 153.07 | 85.05 | 106.46 | 128.14 | 157.62 |
Intensity Change Log 2 | — | 0.145890 | 0.394964 | 0.353319 | — | 0.127131 | 0.411519 | 0.452747 | — | 0.136644 | 0.403174 | 0.403343 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000511 | 0.000954 | 0.001244 | 0.001496 | 0.000279 | 0.000815 | 0.000837 | 0.001217 | 0.000398 | 0.000852 | 0.000969 | 0.001360 |
Bud Neck | 0.004776 | 0.005357 | 0.014321 | 0.017878 | 0.003914 | 0.002662 | 0.027394 | 0.023182 | 0.004357 | 0.003384 | 0.023149 | 0.020470 |
Bud Site | 0.001755 | 0.001859 | 0.003128 | 0.006257 | 0.001000 | 0.001486 | 0.002877 | 0.009499 | 0.001388 | 0.001586 | 0.002959 | 0.007841 |
Cell Periphery | 0.000151 | 0.000210 | 0.000081 | 0.000242 | 0.000142 | 0.000164 | 0.000072 | 0.000159 | 0.000146 | 0.000177 | 0.000075 | 0.000202 |
Cytoplasm | 0.092428 | 0.069493 | 0.005663 | 0.025945 | 0.053846 | 0.030994 | 0.004010 | 0.019493 | 0.073685 | 0.041312 | 0.004546 | 0.022793 |
Cytoplasmic Foci | 0.037250 | 0.029305 | 0.000427 | 0.005895 | 0.010894 | 0.011296 | 0.000161 | 0.001051 | 0.024446 | 0.016123 | 0.000247 | 0.003528 |
Eisosomes | 0.000053 | 0.000103 | 0.000035 | 0.000045 | 0.000037 | 0.000058 | 0.000025 | 0.000031 | 0.000045 | 0.000070 | 0.000028 | 0.000038 |
Endoplasmic Reticulum | 0.002117 | 0.007939 | 0.004755 | 0.007040 | 0.002217 | 0.004912 | 0.003113 | 0.007009 | 0.002166 | 0.005723 | 0.003646 | 0.007025 |
Endosome | 0.011431 | 0.007750 | 0.000597 | 0.005723 | 0.000961 | 0.001950 | 0.000656 | 0.001701 | 0.006345 | 0.003504 | 0.000637 | 0.003758 |
Golgi | 0.015181 | 0.000815 | 0.000319 | 0.003986 | 0.000654 | 0.002524 | 0.000400 | 0.002131 | 0.008124 | 0.002066 | 0.000374 | 0.003080 |
Lipid Particles | 0.010165 | 0.014457 | 0.000331 | 0.001254 | 0.004730 | 0.007723 | 0.000137 | 0.000351 | 0.007524 | 0.009528 | 0.000200 | 0.000812 |
Mitochondria | 0.010947 | 0.005079 | 0.002254 | 0.007200 | 0.003909 | 0.009792 | 0.003860 | 0.003626 | 0.007528 | 0.008529 | 0.003338 | 0.005454 |
Mitotic Spindle | 0.010701 | 0.006567 | 0.041482 | 0.066396 | 0.005129 | 0.005495 | 0.056000 | 0.186935* | 0.007995 | 0.005783 | 0.051285 | 0.125293* |
None | 0.021988 | 0.009864 | 0.004158 | 0.021075 | 0.021297 | 0.026530 | 0.003642 | 0.013687 | 0.021653 | 0.022063 | 0.003809 | 0.017465 |
Nuclear Periphery | 0.003982 | 0.002719 | 0.001787 | 0.003182 | 0.003294 | 0.001481 | 0.002365 | 0.004730 | 0.003648 | 0.001813 | 0.002178 | 0.003939 |
Nuclear Periphery Foci | 0.010186 | 0.006232 | 0.011920 | 0.007562 | 0.011212 | 0.014992 | 0.005521 | 0.016470 | 0.010684 | 0.012644 | 0.007599 | 0.011915 |
Nucleolus | 0.075614 | 0.032529 | 0.114246 | 0.048904 | 0.081776 | 0.089747 | 0.128184 | 0.055471 | 0.078607 | 0.074411 | 0.123658 | 0.052113 |
Nucleus | 0.675775* | 0.779227* | 0.789969* | 0.759277* | 0.791827* | 0.778882* | 0.757791* | 0.645893* | 0.732151* | 0.778975* | 0.768241* | 0.703876* |
Peroxisomes | 0.010294 | 0.006008 | 0.001023 | 0.001707 | 0.001430 | 0.004658 | 0.000503 | 0.000596 | 0.005988 | 0.005020 | 0.000671 | 0.001164 |
Vacuole | 0.003801 | 0.012747 | 0.001855 | 0.007221 | 0.001213 | 0.003239 | 0.002040 | 0.005897 | 0.002544 | 0.005787 | 0.001980 | 0.006574 |
Vacuole Periphery | 0.000896 | 0.000786 | 0.000405 | 0.001714 | 0.000239 | 0.000598 | 0.000414 | 0.000872 | 0.000577 | 0.000649 | 0.000411 | 0.001303 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.75 | -13.08 | -11.38 | -1.15 | -3.84 | -4.29 | -20.24 | -19.02 | -6.33 | -9.83 | -4.56 | -19.83 | -19.41 | -5.51 | -9.62 |
Bud Neck | -1.36 | -11.96 | -12.64 | -10.45 | -2.98 | 1.93 | -22.01 | -13.25 | -14.09 | 0.30 | 1.42 | -25.17 | -18.26 | -18.65 | -0.21 |
Bud Site | -1.87 | -3.52 | -6.13 | -4.53 | -2.82 | -1.01 | -6.24 | -6.95 | -6.40 | -4.80 | -1.36 | -6.73 | -9.27 | -8.22 | -5.77 |
Cell Periphery | -2.54 | 6.85 | -1.77 | 1.39 | -5.70 | -0.87 | 6.29 | 0.30 | 1.08 | -7.03 | -1.77 | 9.91 | -1.21 | 1.01 | -8.83 |
Cytoplasm | 1.86 | 21.43 | 15.61 | 4.09 | -7.41 | 4.12 | 16.91 | 9.10 | 2.24 | -7.34 | 6.03 | 27.29 | 17.80 | 4.31 | -10.71 |
Cytoplasmic Foci | 0.82 | 15.46 | 13.94 | 4.13 | -2.42 | 0.19 | 10.07 | 9.72 | 5.00 | -1.53 | 2.83 | 18.36 | 16.60 | 6.06 | -3.19 |
Eisosomes | -3.66 | 6.38 | 0.98 | 3.83 | -4.99 | -3.84 | 7.35 | -2.08 | 2.62 | -8.38 | -4.42 | 11.31 | -0.48 | 4.24 | -10.81 |
Endoplasmic Reticulum | -6.02 | -11.56 | -14.74 | 0.22 | -7.72 | -4.14 | -4.06 | -12.28 | -4.75 | -10.73 | -6.71 | -9.61 | -18.91 | -4.22 | -14.33 |
Endosome | 0.74 | 8.19 | 5.16 | 1.08 | -3.41 | -1.76 | 3.92 | -2.02 | 0.90 | -4.58 | 1.89 | 8.58 | 4.78 | 0.93 | -4.62 |
Golgi | 7.20 | 7.55 | 6.12 | -1.40 | -2.11 | -1.68 | 1.26 | -0.49 | 1.28 | -0.95 | 4.33 | 7.59 | 5.70 | 0.64 | -2.27 |
Lipid Particles | -1.05 | 10.17 | 9.05 | 3.17 | -2.83 | -1.28 | 7.27 | 6.63 | 3.80 | -6.36 | -0.98 | 12.54 | 11.17 | 4.86 | -4.52 |
Mitochondria | 3.13 | 6.16 | 3.16 | -0.27 | -3.04 | -1.61 | 0.38 | -0.00 | 1.64 | -0.77 | -0.35 | 5.24 | 2.91 | 1.41 | -2.63 |
Mitotic Spindle | -0.20 | -10.04 | -12.60 | -9.74 | -4.94 | -0.28 | -17.06 | -18.30 | -18.04 | -10.62 | 0.13 | -19.47 | -21.95 | -21.05 | -10.82 |
None | 6.43 | 13.61 | 4.33 | -1.73 | -5.19 | -1.38 | 11.41 | 6.18 | 4.53 | -4.67 | -0.01 | 17.94 | 7.31 | 3.63 | -7.04 |
Nuclear Periphery | 1.71 | 4.68 | -1.12 | -2.46 | -4.84 | 5.35 | 2.15 | -4.48 | -8.95 | -6.69 | 5.94 | 4.59 | -3.72 | -8.64 | -7.71 |
Nuclear Periphery Foci | -0.09 | -0.22 | 0.46 | 0.29 | 0.65 | -1.64 | 5.00 | 0.67 | 1.96 | -4.31 | -1.68 | 4.32 | 0.80 | 2.01 | -3.35 |
Nucleolus | 3.54 | -9.15 | 3.10 | -1.91 | 11.61 | -0.46 | -14.54 | 2.92 | 2.45 | 16.83 | 1.12 | -17.59 | 4.25 | 1.46 | 21.02 |
Nucleus | -3.19 | -4.60 | -3.24 | 1.52 | 0.93 | 0.88 | 10.61 | 11.89 | 7.59 | 4.50 | -2.82 | 3.78 | 5.94 | 6.35 | 2.98 |
Peroxisomes | 0.77 | 6.79 | 6.68 | 3.67 | -0.24 | -2.71 | 4.44 | 4.15 | 3.64 | -0.54 | 0.22 | 7.70 | 7.27 | 4.77 | -1.47 |
Vacuole | -3.30 | 2.47 | -5.22 | 1.32 | -6.70 | -1.79 | -1.00 | -7.45 | -4.84 | -7.13 | -3.07 | 2.14 | -9.01 | -3.08 | -9.93 |
Vacuole Periphery | 0.19 | 1.97 | -0.38 | -0.64 | -2.34 | -2.63 | -1.36 | -2.98 | 0.11 | -1.95 | -0.60 | 1.68 | -1.28 | -0.78 | -3.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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